Standard name | |
---|---|
Human Ortholog | |
Description | Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 5 | 18 | 16 | 11 | 26 | 17 | 27 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 1 | 3 | 0 | 3 | 6 | 5 | 3 | 6 | 4 | 5 | 1 | 1 | 3 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 6 | 5 | 16 | 15 | 44 | 47 | 21 | 42 | 29 | 47 | 6 | 7 | 7 | 6 | 7 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 310 | 145 | 332 | 214 | 377 | 347 | 412 | 451 | 234 | 298 | 193 | 308 | 227 | 253 | 251 | 216 | 167 | 132 | 293 | 139 | 337 | 130 | 172 | 246 |
Endoplasmic Reticulum | 3 | 3 | 11 | 5 | 37 | 20 | 65 | 86 | 36 | 40 | 31 | 39 | 1 | 3 | 4 | 9 | 9 | 9 | 1 | 1 | 13 | 2 | 7 | 23 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 4 | 4 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3 | 9 | 14 | 15 | 16 | 24 | 39 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 3 | 4 | 3 | 1 | 3 | 12 | 11 | 7 | 7 | 4 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 2 | 3 | 1 | 1 | 1 |
Unique Cell Count | 311 | 148 | 346 | 225 | 410 | 374 | 508 | 584 | 306 | 385 | 271 | 401 | 228 | 257 | 257 | 228 | 173 | 136 | 302 | 148 | 365 | 144 | 189 | 282 |
Labelled Cell Count | 318 | 149 | 355 | 232 | 435 | 398 | 558 | 633 | 333 | 438 | 310 | 473 | 235 | 265 | 267 | 238 | 185 | 146 | 302 | 148 | 365 | 144 | 189 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 69.5 | 61.6 | 37.9 | 32.5 | 36.4 | 27.2 | 29.8 | 28.5 | 22.9 | 27.2 | 21.0 | 25.7 | 50.0 | 43.7 | 37.3 | 79.3 | 83.2 | 77.9 | 52.1 | 56.4 | 60.2 |
Std Deviation (1e-4) | 20.2 | 19.4 | 11.6 | 12.3 | 10.1 | 10.8 | 8.7 | 7.8 | 8.6 | 8.1 | 7.5 | 8.9 | 13.6 | 11.4 | 9.3 | 24.4 | 28.7 | 27.5 | 19.7 | 18.8 | 22.0 |
Intensity Change (Log2) | – | – | – | -0.22 | -0.06 | -0.48 | -0.35 | -0.41 | -0.73 | -0.48 | -0.85 | -0.56 | 0.4 | 0.2 | -0.02 | 1.06 | 1.13 | 1.04 | 0.46 | 0.57 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.5 | 3.1 | 3.6 | 4.9 | 4.7 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.8 | 1.8 | 4.2 | 4.0 | 3.3 | 5.0 | 4.8 | 5.3 | 0 | 0.8 | 0.8 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -2.3 | -1.8 | -6.4 | -7.6 | -7.3 | -7.3 | -8.6 | -7.4 | 2.7 | 1.8 | 1.2 | -0.7 | 0.3 | 0.6 |
Endoplasmic Reticulum | -0.7 | 3.3 | 1.4 | 4.8 | 5.6 | 4.2 | 3.8 | 4.0 | 3.6 | 0 | -1.6 | -1.3 | 0.5 | 1.1 | 1.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.9 | 4.2 | 3.8 | 5.6 | 6.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.4 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9351 | 3.8895 | 3.4464 | 2.6892 | 1.9451 | 3.4371 | 3.5382 | 4.7462 | 3.9956 | 3.7927 | 2.7138 | 4.5114 | 4.0888 | 5.214 | 4.7802 | 4.4038 | 5.0705 | 5.1451 |
Actin | 0.0252 | 0.0057 | 0.0158 | 0.0059 | 0.0036 | 0.0003 | 0.0069 | 0.0005 | 0.0092 | 0.001 | 0.0013 | 0.0044 | 0.0027 | 0.0006 | 0.0011 | 0.0009 | 0.0073 | 0.004 |
Bud | 0.0009 | 0.0008 | 0.0004 | 0.0019 | 0.0043 | 0.0002 | 0.0007 | 0.001 | 0.0088 | 0.0017 | 0.002 | 0.0004 | 0.0044 | 0.0006 | 0.0016 | 0.0035 | 0.0012 | 0.0011 |
Bud Neck | 0.0008 | 0.0004 | 0.0007 | 0.0019 | 0.0005 | 0.0006 | 0.0012 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0.0006 | 0.0043 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0007 |
Bud Periphery | 0.0013 | 0.0007 | 0.0004 | 0.0008 | 0.0063 | 0.0008 | 0.0009 | 0.0004 | 0.0053 | 0.0022 | 0.0036 | 0.001 | 0.0037 | 0.0005 | 0.0013 | 0.0024 | 0.0014 | 0.0015 |
Bud Site | 0.0051 | 0.0179 | 0.0166 | 0.0029 | 0.0018 | 0.0001 | 0.0033 | 0.0065 | 0.0044 | 0.0009 | 0.0057 | 0.0001 | 0.0055 | 0.0075 | 0.0056 | 0.001 | 0.0016 | 0.0002 |
Cell Periphery | 0.0008 | 0.0009 | 0.0012 | 0.0002 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.612 | 0.82 | 0.8077 | 0.8692 | 0.6939 | 0.821 | 0.6323 | 0.828 | 0.7565 | 0.6838 | 0.7629 | 0.7903 | 0.6572 | 0.7513 | 0.7628 | 0.8353 | 0.6014 | 0.8203 |
Cytoplasmic Foci | 0.0286 | 0.0178 | 0.0105 | 0.0078 | 0.0253 | 0.0034 | 0.03 | 0.0081 | 0.0181 | 0.0282 | 0.0201 | 0.0108 | 0.0152 | 0.0135 | 0.0206 | 0.0208 | 0.0222 | 0.0143 |
Eisosomes | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0211 | 0.0043 | 0.0085 | 0.0173 | 0.0138 | 0.0034 | 0.0064 | 0.0024 | 0.0027 | 0.0022 | 0.0022 | 0.0048 | 0.0086 | 0.0036 | 0.0028 | 0.0072 | 0.0058 | 0.0019 |
Endosome | 0.0184 | 0.0064 | 0.0087 | 0.0157 | 0.0729 | 0.0086 | 0.044 | 0.0049 | 0.0141 | 0.015 | 0.0285 | 0.0126 | 0.0064 | 0.0055 | 0.0121 | 0.0401 | 0.0272 | 0.0089 |
Golgi | 0.0049 | 0.0045 | 0.0028 | 0.001 | 0.0339 | 0.0017 | 0.0083 | 0.0004 | 0.0066 | 0.0034 | 0.0051 | 0.0058 | 0.0011 | 0.0006 | 0.0009 | 0.0024 | 0.0079 | 0.0007 |
Lipid Particles | 0.0107 | 0.0004 | 0.0031 | 0.0001 | 0.0383 | 0.0001 | 0.0023 | 0.0001 | 0.0054 | 0.0122 | 0.0005 | 0.0026 | 0.0009 | 0.0007 | 0.0004 | 0.0004 | 0.0008 | 0.0001 |
Mitochondria | 0.0107 | 0.0045 | 0.0057 | 0.0039 | 0.0113 | 0.0235 | 0.002 | 0.0002 | 0.0069 | 0.0029 | 0.002 | 0.0041 | 0.001 | 0.0004 | 0.0005 | 0.0011 | 0.0056 | 0.0005 |
None | 0.1983 | 0.1055 | 0.0979 | 0.0436 | 0.0136 | 0.1279 | 0.238 | 0.1412 | 0.1518 | 0.2146 | 0.1549 | 0.1543 | 0.2755 | 0.2093 | 0.1778 | 0.0615 | 0.3049 | 0.1419 |
Nuclear Periphery | 0.0079 | 0.0007 | 0.0012 | 0.0049 | 0.0136 | 0.0004 | 0.0015 | 0.0004 | 0.0007 | 0.0011 | 0.001 | 0.0005 | 0.0008 | 0.0005 | 0.0006 | 0.0019 | 0.001 | 0.0003 |
Nucleolus | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0056 | 0.0014 | 0.0138 | 0.0107 | 0.0101 | 0.0008 | 0.0014 | 0.0011 | 0.0013 | 0.0012 | 0.0012 | 0.0006 | 0.0009 | 0.001 | 0.0011 | 0.0015 | 0.0008 | 0.0007 |
Peroxisomes | 0.0219 | 0.0004 | 0.0003 | 0.0008 | 0.0352 | 0 | 0.0076 | 0.0001 | 0.0019 | 0.0183 | 0.001 | 0.0044 | 0.007 | 0.0001 | 0.0016 | 0.0002 | 0.0049 | 0.0002 |
Punctate Nuclear | 0.0182 | 0.0008 | 0.0007 | 0.0009 | 0.0018 | 0.0001 | 0.0021 | 0.0002 | 0.0008 | 0.0007 | 0.0005 | 0.0002 | 0.0006 | 0.0004 | 0.0008 | 0.0006 | 0.0006 | 0.0003 |
Vacuole | 0.0043 | 0.0062 | 0.0034 | 0.0091 | 0.0132 | 0.0037 | 0.0093 | 0.0036 | 0.0039 | 0.0087 | 0.0059 | 0.0019 | 0.0029 | 0.0026 | 0.0063 | 0.016 | 0.0038 | 0.0017 |
Vacuole Periphery | 0.0009 | 0.0003 | 0.0005 | 0.0015 | 0.0048 | 0.003 | 0.001 | 0.0003 | 0.0005 | 0.0012 | 0.0011 | 0.0004 | 0.0003 | 0.0002 | 0.0007 | 0.0026 | 0.0008 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3267 | 14.1474 | 17.0094 | 26.4926 | 60.6478 | 11.2121 | 14.3949 | 14.5429 | 16.5221 | 25.2521 |
Translational Efficiency | 0.5389 | 0.3835 | 0.4233 | 0.4594 | 0.5846 | 0.2772 | 0.2668 | 0.2469 | 0.2624 | 0.3934 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
151 | 210 | 1723 | 1019 | 1449 | 2028 | 1910 | 1641 | 1600 | 2238 | 3633 | 2660 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3750.79 | 2442.91 | 2134.21 | 815.47 | 3527.61 | 2917.92 | 2719.02 | 814.14 | 3548.67 | 2873.35 | 2441.67 | 814.65 |
Standard Deviation | 1035.28 | 799.68 | 847.54 | 132.13 | 990.72 | 1001.70 | 1204.61 | 130.32 | 997.14 | 994.21 | 1090.33 | 131.02 |
Intensity Change Log 2 | — | -0.618594 | -0.813492 | -2.201491 | — | -0.273751 | -0.375604 | -2.115342 | — | -0.441165 | -0.584676 | -2.159095 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000278 | 0.000822 | 0.000284 | 0.002577 | 0.000072 | 0.000145 | 0.000215 | 0.000275 | 0.000091 | 0.000209 | 0.000248 | 0.001156 |
Bud Neck | 0.001609 | 0.001529 | 0.002398 | 0.011914 | 0.000429 | 0.000645 | 0.001677 | 0.010736 | 0.000541 | 0.000728 | 0.002019 | 0.011188 |
Bud Site | 0.018170 | 0.038830 | 0.013572 | 0.059981 | 0.003762 | 0.008387 | 0.016167 | 0.049486 | 0.005122 | 0.011244 | 0.014936 | 0.053507 |
Cell Periphery | 0.085349 | 0.011849 | 0.001077 | 0.000219 | 0.005870 | 0.001622 | 0.003039 | 0.000162 | 0.013371 | 0.002582 | 0.002109 | 0.000184 |
Cytoplasm | 0.263968* | 0.737234* | 0.875110* | 0.479663* | 0.869461* | 0.897268* | 0.841993* | 0.572694* | 0.812318* | 0.882252* | 0.857699* | 0.537055* |
Cytoplasmic Foci | 0.018400 | 0.054252 | 0.020502 | 0.039972 | 0.009459 | 0.014658 | 0.015356 | 0.026464 | 0.010303 | 0.018373 | 0.017797 | 0.031639 |
Eisosomes | 0.000008 | 0.000005 | 0.000002 | 0.000059 | 0.000001 | 0.000001 | 0.000003 | 0.000039 | 0.000002 | 0.000001 | 0.000002 | 0.000047 |
Endoplasmic Reticulum | 0.003893 | 0.002106 | 0.000235 | 0.001554 | 0.000768 | 0.000396 | 0.000287 | 0.000714 | 0.001063 | 0.000557 | 0.000262 | 0.001036 |
Endosome | 0.002931 | 0.006346 | 0.001228 | 0.016531 | 0.000782 | 0.001494 | 0.001544 | 0.006239 | 0.000984 | 0.001949 | 0.001394 | 0.010182 |
Golgi | 0.175081* | 0.063261 | 0.006432 | 0.006526 | 0.032578 | 0.022135 | 0.011663 | 0.003021 | 0.046027 | 0.025994 | 0.009182 | 0.004364 |
Lipid Particles | 0.029566 | 0.017668 | 0.002141 | 0.003800 | 0.008182 | 0.003106 | 0.005937 | 0.000886 | 0.010200 | 0.004472 | 0.004137 | 0.002002 |
Mitochondria | 0.000253 | 0.000081 | 0.000207 | 0.007794 | 0.000139 | 0.000152 | 0.000271 | 0.001017 | 0.000150 | 0.000145 | 0.000240 | 0.003613 |
Mitotic Spindle | 0.001978 | 0.003691 | 0.008923 | 0.068213 | 0.001326 | 0.002906 | 0.005357 | 0.026687 | 0.001388 | 0.002980 | 0.007049 | 0.042594 |
None | 0.001101 | 0.000686 | 0.002463 | 0.004726 | 0.001800 | 0.002199 | 0.001958 | 0.005633 | 0.001734 | 0.002057 | 0.002197 | 0.005286 |
Nuclear Periphery | 0.000737 | 0.000225 | 0.000225 | 0.001050 | 0.000195 | 0.000119 | 0.000165 | 0.000877 | 0.000246 | 0.000129 | 0.000194 | 0.000944 |
Nuclear Periphery Foci | 0.009582 | 0.006979 | 0.003128 | 0.001121 | 0.007000 | 0.005050 | 0.003998 | 0.000869 | 0.007243 | 0.005231 | 0.003585 | 0.000965 |
Nucleolus | 0.000128 | 0.000040 | 0.000102 | 0.000916 | 0.000043 | 0.000071 | 0.000096 | 0.000621 | 0.000051 | 0.000068 | 0.000099 | 0.000734 |
Nucleus | 0.004436 | 0.004109 | 0.038287 | 0.124651 | 0.005706 | 0.008357 | 0.020262 | 0.150666* | 0.005586 | 0.007958 | 0.028811 | 0.140700* |
Peroxisomes | 0.000135 | 0.000243 | 0.000092 | 0.004516 | 0.000029 | 0.000058 | 0.000093 | 0.000770 | 0.000039 | 0.000076 | 0.000092 | 0.002205 |
Vacuole | 0.139391 | 0.027740 | 0.019835 | 0.151991 | 0.027869 | 0.019733 | 0.058778 | 0.140563 | 0.038394 | 0.020484 | 0.040309 | 0.144941 |
Vacuole Periphery | 0.243006* | 0.022305 | 0.003758 | 0.012223 | 0.024528 | 0.011497 | 0.011140 | 0.001582 | 0.045147 | 0.012511 | 0.007639 | 0.005659 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.03 | -0.42 | -2.20 | -0.70 | -2.07 | -3.67 | -8.89 | -4.45 | -2.33 | 0.18 | -5.20 | -5.11 | -3.38 | -2.51 | -1.94 |
Bud Neck | 0.16 | -2.17 | -5.43 | -6.34 | -4.74 | -2.79 | -12.43 | -8.30 | -8.01 | -6.15 | -2.25 | -13.56 | -10.91 | -10.56 | -7.60 |
Bud Site | -3.55 | 1.08 | -1.46 | 2.48 | -3.27 | -5.93 | -13.04 | -8.09 | -6.19 | -2.57 | -6.69 | -11.87 | -9.68 | -6.44 | -4.17 |
Cell Periphery | 7.74 | 9.06 | 9.17 | 5.98 | 11.22 | 8.10 | 5.31 | 11.29 | 11.88 | 11.62 | 9.27 | 9.83 | 11.61 | 10.99 | 14.51 |
Cytoplasm | -15.19 | -24.16 | -5.10 | 15.66 | 44.26* | -3.74 | 5.88 | 41.32 | 50.07 | 38.87* | -8.14 | -3.51 | 38.74 | 60.16 | 58.36* |
Cytoplasmic Foci | -5.02 | -0.96 | -1.48 | 4.62 | -1.07 | -5.10 | -7.05 | -9.24 | -4.58 | -2.72 | -6.90 | -9.05 | -9.98 | -2.65 | -2.24 |
Eisosomes | 2.00 | 3.16 | -16.53 | -20.10 | -20.98 | 1.16 | -8.24 | -33.42* | -33.70* | -31.45* | 2.18 | -4.28 | -37.21 | -37.95* | -36.01* |
Endoplasmic Reticulum | 1.26 | 2.76 | 1.54 | 0.41 | -6.59 | 2.11 | 3.02 | -2.67 | -8.96 | -15.12 | 2.42 | 4.12 | -1.84 | -6.94 | -11.92 |
Endosome | -3.18 | 3.97 | -3.82 | -0.39 | -5.77 | -4.23 | -5.50 | -6.13 | -4.41 | -4.31 | -5.19 | -4.22 | -8.30 | -5.92 | -7.25 |
Golgi | 7.00 | 12.16 | 12.47 | 7.80 | 5.17 | 4.37 | 10.61 | 15.67 | 13.46 | 10.90 | 7.01 | 15.19 | 18.15 | 15.34 | 11.85 |
Lipid Particles | 2.25 | 7.00 | 7.01 | 4.26 | 0.41 | 5.97 | 2.49 | 8.96 | 6.99 | 8.69 | 6.14 | 6.90 | 10.36 | 6.36 | 7.46 |
Mitochondria | 4.06 | -0.45 | -3.05 | -3.26 | -3.03 | -0.73 | -2.31 | -8.06 | -7.70 | -4.32 | 0.02 | -3.37 | -4.32 | -4.31 | -3.85 |
Mitotic Spindle | -2.98 | -9.22 | -8.20 | -7.77 | -6.08 | -2.51 | -6.37 | -7.93 | -7.21 | -4.65 | -2.76 | -10.03 | -11.32 | -10.58 | -7.54 |
None | 2.78 | -8.72 | -8.72 | -9.67 | -5.65 | -0.79 | -0.45 | -5.65 | -3.49 | -4.66 | -0.71 | -2.11 | -8.37 | -4.91 | -6.84 |
Nuclear Periphery | 2.94 | 3.64 | -7.03 | -10.00 | -13.81 | 2.78 | -0.57 | -18.84 | -20.03 | -19.11 | 3.92 | 0.20 | -22.38 | -24.47 | -23.28 |
Nuclear Periphery Foci | 1.90 | 6.13 | 8.56 | 6.12 | 6.07 | 2.90 | 5.03 | 11.88 | 9.22 | 7.48 | 3.23 | 6.99 | 13.25 | 10.22 | 9.71 |
Nucleolus | 4.11 | 0.71 | -4.66 | -5.51 | -4.87 | -1.72 | -8.72 | -5.09 | -4.80 | -4.52 | -1.20 | -8.94 | -7.04 | -6.73 | -6.34 |
Nucleus | 0.10 | -15.28 | -24.35 | -25.43 | -18.76 | -4.41 | -17.10 | -35.25 | -34.68 | -30.97* | -4.17 | -26.63 | -43.16 | -42.54 | -35.45 |
Peroxisomes | -1.81 | 0.85 | -4.30 | -4.04 | -4.41 | -3.38 | -6.62 | -5.82 | -5.43 | -4.91 | -3.75 | -6.54 | -5.97 | -5.75 | -5.62 |
Vacuole | 7.96 | 8.48 | -9.80 | -27.04 | -29.27 | 3.17 | -10.56 | -31.05 | -33.12 | -23.72 | 6.47 | -2.63 | -37.88 | -43.61 | -37.83 |
Vacuole Periphery | 11.10 | 12.35 | 12.31 | 4.00 | -0.21 | 6.32 | 6.92 | 12.80 | 11.45 | 8.91 | 10.57 | 12.73 | 14.46 | 9.89 | 6.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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