Standard name
Human Ortholog
Description Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 – 0 0.05 0 0.05 0 0 0.05 0.14 0.06 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.94 0.91 0.96 0.96 0.88 – 0.8 0.78 0.7 0.79 0.95 0.99 0.98 0.86 0.9 0.92 0.98 0.96 0.82 0.96 0.95 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.12 – 0.25 0.2 0.45 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.09 0 0 0 – 0 0 0 0 0 0 0 0.07 0.1 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 5 0 0 1 0 0 0 0 0 0 0 0 1 0 2 2
Bud 0 0 0 1 0 0 1 – 4 2 3 3 0 0 0 0 0 0 0 0 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 5 5 6 3 7 – 5 13 4 11 2 7 13 16 5 9 0 0 0 0 0 0
Cytoplasm 97 225 133 88 176 116 205 – 167 200 119 179 112 242 281 98 72 97 114 221 120 100 223 236
Endoplasmic Reticulum 0 6 1 0 3 1 1 – 2 5 1 2 0 2 1 6 4 3 0 2 7 0 0 0
Endosome 0 0 0 1 0 0 2 – 3 1 0 0 0 1 2 0 1 0 0 2 0 0 0 1
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 1
Mitochondria 3 0 1 3 4 7 28 – 53 51 76 55 3 0 3 2 1 2 0 1 2 0 1 1
Nucleus 1 0 2 1 3 2 2 – 1 4 1 4 4 0 1 1 1 1 0 0 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 5 9 1 0 7 – 4 5 3 7 1 1 2 8 8 8 0 1 6 0 0 2
Unique Cell Count 98 230 142 97 184 121 233 210 255 170 227 118 245 286 114 80 105 117 232 147 105 235 259
Labelled Cell Count 101 237 148 108 193 129 254 244 281 207 263 122 253 304 135 93 122 117 232 147 105 235 259


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 9.4 8.1 8.9 8.6 7.3 6.9 – 6.4 6.3 5.8 6.0 7.9 8.5 8.0 9.7 9.6 9.3 8.0 8.3 8.6
Std Deviation (1e-4) 0.9 1.0 1.5 2.9 2.9 1.9 1.7 – 1.8 1.2 1.7 1.7 1.2 1.4 1.2 1.9 2.1 1.5 1.7 1.7 2.0
Intensity Change (Log2) – – – 0.12 0.08 -0.16 -0.23 – -0.35 -0.36 -0.5 -0.43 -0.05 0.06 -0.03 0.25 0.24 0.19 -0.03 0.04 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0.7 0 0.6 0 0 0.5 3.0 0 1.7
Cytoplasm -0.8 0.8 0.8 -1.8 – -3.7 -4.0 -5.3 -3.8 0.4 2.8 2.5 -2.1 -1.0 -0.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.0 – 6.3 5.5 9.0 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.9 0 0 0 – 0 0 0 0 0 0 0 1.3 0 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4914 2.6884 2.2779 1.8538 1.4501 2.2627 1.741 3.0285 2.7245 3.0157 2.3212 3.18 3.6799 5.0235 4.3095 4.3018 4.4382 5.0101
Actin 0.03 0.0074 0.0215 0.0002 0.0704 0.0148 0.0147 0.0007 0.004 0.0001 0.0171 0.0052 0.0045 0.0003 0.0002 0.0002 0.044 0.0034
Bud 0.0006 0.0003 0.0011 0.0003 0.0016 0.0003 0.0007 0.0009 0.0036 0.0003 0.0023 0.0006 0.0003 0.0005 0.0004 0.0005 0.0004 0.0002
Bud Neck 0.0012 0.0002 0.0006 0.0004 0.0023 0.0023 0.0017 0.0002 0.0016 0.0003 0.0006 0.0008 0.0003 0.0002 0.0003 0.0003 0.0005 0.0008
Bud Periphery 0.0005 0.0002 0.001 0.0001 0.0022 0.0003 0.0007 0.001 0.0025 0.0001 0.0026 0.0005 0.0002 0.0003 0.0001 0.0003 0.0003 0.0002
Bud Site 0.0056 0.0022 0.0043 0.0003 0.0029 0.0008 0.0023 0.0016 0.0183 0.0003 0.0017 0.0001 0.0004 0.0012 0.0007 0.0002 0.0006 0.0002
Cell Periphery 0.0008 0.0002 0.0004 0.0001 0.0003 0.0002 0.0005 0.0003 0.0004 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.665 0.9235 0.846 0.8708 0.6382 0.7969 0.7221 0.9015 0.7896 0.929 0.7404 0.9101 0.8808 0.9506 0.9307 0.9243 0.8933 0.9523
Cytoplasmic Foci 0.0424 0.0106 0.0196 0.0431 0.0272 0.0324 0.0508 0.0149 0.0359 0.0137 0.0514 0.0107 0.0135 0.0098 0.0145 0.0082 0.009 0.0091
Eisosomes 0.0006 0.0001 0.0001 0 0.0012 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0211 0.0027 0.0053 0.0043 0.0113 0.0149 0.02 0.0035 0.0076 0.0033 0.0035 0.0035 0.0178 0.0074 0.0081 0.0141 0.0039 0.0084
Endosome 0.0736 0.013 0.0269 0.056 0.0555 0.0559 0.0429 0.0111 0.0288 0.0266 0.0527 0.0156 0.0305 0.0106 0.0236 0.0255 0.0212 0.0141
Golgi 0.0272 0.0015 0.0144 0.0025 0.0432 0.0117 0.0086 0.0014 0.0026 0.0006 0.0146 0.0011 0.0023 0.0009 0.001 0.0007 0.0013 0.0012
Lipid Particles 0.017 0.0007 0.0086 0.0003 0.0204 0.0171 0.0132 0.0002 0.0016 0.0001 0.0096 0.0001 0.0015 0.0001 0.0003 0.0001 0.0008 0.0002
Mitochondria 0.0233 0.0135 0.0052 0.0007 0.0596 0.0267 0.0125 0.0006 0.0012 0.0002 0.0061 0.002 0.0006 0.0002 0.0004 0.0003 0.0005 0.0008
None 0.0598 0.019 0.0311 0.0068 0.0031 0.0071 0.0612 0.0565 0.0849 0.0098 0.0658 0.0446 0.0292 0.0125 0.0062 0.0041 0.0059 0.004
Nuclear Periphery 0.0047 0.0004 0.0013 0.0012 0.0049 0.002 0.0043 0.0006 0.0017 0.0008 0.002 0.0004 0.0027 0.0007 0.0009 0.0023 0.0007 0.0008
Nucleolus 0.0005 0.0001 0.0004 0 0.0004 0.0002 0.0006 0 0.0002 0 0.0003 0 0.0001 0 0 0 0 0
Nucleus 0.0025 0.0008 0.0014 0.0015 0.0041 0.0009 0.0024 0.0009 0.0013 0.0011 0.0018 0.0006 0.0013 0.0009 0.0015 0.0017 0.001 0.0009
Peroxisomes 0.0078 0.0007 0.0017 0.0006 0.0398 0.0083 0.024 0.0002 0.0054 0 0.0166 0.0001 0.0002 0.0001 0.0002 0 0.0022 0.0001
Punctate Nuclear 0.0059 0.0005 0.0011 0.0005 0.0012 0.0012 0.0066 0.0002 0.0019 0.0001 0.0051 0.0002 0.0008 0.0001 0.0003 0.0002 0.0002 0.0002
Vacuole 0.0075 0.002 0.0064 0.0086 0.0074 0.004 0.0085 0.0034 0.006 0.0119 0.0044 0.0028 0.0114 0.003 0.0091 0.0148 0.0122 0.0023
Vacuole Periphery 0.0023 0.0004 0.0015 0.0018 0.0027 0.0019 0.0013 0.0005 0.0008 0.0016 0.0013 0.0007 0.0013 0.0005 0.0012 0.002 0.0019 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4396 11.2033 7.229 15.4449 15.5425 16.8823 14.1595 15.457 15.6163 15.6723
Translational Efficiency 1.0139 1.0483 1.4375 1.0703 0.7998 0.7512 1.2289 0.7974 0.8487 0.7581

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2037 1355 247 100 1475 2115 380 225 3512 3470 627 325

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.62 836.34 1156.45 1129.68 865.69 907.99 1120.25 1236.74 804.17 880.01 1134.51 1203.80
Standard Deviation 89.48 151.82 147.95 169.29 105.62 120.45 130.82 181.89 109.86 138.08 138.96 184.83
Intensity Change Log 2 — 0.138812 0.606353 0.572564 — 0.068826 0.371898 0.514620 — 0.101958 0.486229 0.541991

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000086 0.000132 0.000277 0.000335 0.000145 0.000200 0.000209 0.000082 0.000111 0.000173 0.000236 0.000159
Bud Neck 0.004691 0.002770 0.001154 0.002131 0.003587 0.004485 0.001694 0.011911 0.004228 0.003816 0.001481 0.008902
Bud Site 0.012130 0.011814 0.012435 0.029160 0.010979 0.019337 0.011369 0.026226 0.011646 0.016400 0.011789 0.027129
Cell Periphery 0.000125 0.000080 0.000135 0.000253 0.000150 0.000098 0.000121 0.000226 0.000135 0.000091 0.000127 0.000234
Cytoplasm 0.674956* 0.709070* 0.759857* 0.501092* 0.660802* 0.653599* 0.501372* 0.833174* 0.669012* 0.675260* 0.603199* 0.730995*
Cytoplasmic Foci 0.163804 0.129062 0.085375 0.098096 0.199475* 0.176652 0.095296 0.006790 0.178785 0.158069 0.091387 0.034884
Eisosomes 0.000045 0.000023 0.000019 0.000037 0.000057 0.000039 0.000038 0.000012 0.000050 0.000033 0.000030 0.000020
Endoplasmic Reticulum 0.001221 0.001877 0.000854 0.000513 0.002547 0.001169 0.001106 0.000021 0.001778 0.001445 0.001007 0.000172
Endosome 0.015240 0.017329 0.006550 0.012467 0.014196 0.019177 0.013202 0.000567 0.014802 0.018455 0.010581 0.004229
Golgi 0.006547 0.003136 0.000575 0.000418 0.006172 0.009158 0.000356 0.000593 0.006389 0.006807 0.000443 0.000539
Lipid Particles 0.002432 0.002323 0.000986 0.001444 0.002186 0.001483 0.001420 0.000136 0.002329 0.001811 0.001249 0.000539
Mitochondria 0.001676 0.000737 0.000021 0.000011 0.000615 0.001767 0.000034 0.000469 0.001231 0.001365 0.000029 0.000328
Mitotic Spindle 0.001322 0.001379 0.000577 0.000226 0.001609 0.004930 0.000168 0.000074 0.001443 0.003544 0.000329 0.000121
None 0.003044 0.001747 0.001202 0.000143 0.001706 0.000698 0.002307 0.006333 0.002482 0.001108 0.001872 0.004429
Nuclear Periphery 0.000106 0.000232 0.000138 0.000135 0.000052 0.000041 0.000198 0.000015 0.000083 0.000115 0.000174 0.000052
Nuclear Periphery Foci 0.000411 0.001167 0.007056 0.004857 0.000478 0.000246 0.004342 0.000059 0.000439 0.000606 0.005411 0.001535
Nucleolus 0.000423 0.000673 0.000084 0.000095 0.000210 0.000158 0.000143 0.000351 0.000333 0.000359 0.000120 0.000272
Nucleus 0.018453 0.020662 0.026301 0.010420 0.012388 0.007148 0.019813 0.029964 0.015906 0.012425 0.022369 0.023951
Peroxisomes 0.001020 0.001693 0.000438 0.000780 0.001094 0.001571 0.000402 0.000722 0.001051 0.001618 0.000416 0.000740
Vacuole 0.088089 0.091337 0.095680 0.337049* 0.078977 0.093732 0.345959* 0.081492 0.084262 0.092797 0.247364* 0.160125
Vacuole Periphery 0.004179 0.002755 0.000287 0.000337 0.002577 0.004312 0.000452 0.000782 0.003506 0.003704 0.000387 0.000645

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.14 -6.01 -4.09 -3.23 -0.08 -1.91 -1.76 3.51 4.69 3.75 -3.76 -5.34 -2.02 0.84 3.07
Bud Neck 4.61 11.25 7.31 2.19 -2.50 -2.46 6.47 -2.80 -2.48 -3.52 1.46 12.19 -2.19 -2.40 -3.62
Bud Site 0.30 -0.24 -2.06 -2.11 -1.96 -6.67 -0.14 -2.75 -1.10 -2.54 -5.47 -0.08 -3.33 -2.14 -3.17
Cell Periphery 5.75 -0.74 -0.79 -1.41 -0.64 2.95 2.00 -1.82 -3.70 -3.18 5.13 1.36 -2.16 -3.64 -2.66
Cytoplasm -3.82 -3.69 6.29 7.36 7.49 0.88 10.64 -14.88 -15.99 -19.22 -1.00 6.87 -4.26 -3.83 -8.06
Cytoplasmic Foci 6.61 11.82 8.33 4.27 -0.85 3.84 16.47 40.50 44.95 16.33 5.26 19.68 33.37 29.10 10.58
Eisosomes 8.44 7.76 2.45 -2.43 -2.61 2.34 2.82 6.75 6.75 8.92 4.44 5.44 9.04 4.92 5.17
Endoplasmic Reticulum -1.72 1.23 4.52 4.24 2.67 2.12 2.59 4.25 4.42 4.68 1.00 2.84 6.08 6.28 5.66
Endosome -1.63 7.95 3.96 4.90 -1.93 -4.77 2.47 19.72 23.35 11.23 -4.47 6.38 16.53 19.75 7.63
Golgi 6.51 16.97 17.58 6.93 2.16 -4.11 19.78 17.97 12.75 -2.23 -0.81 25.03 23.72 14.21 -1.12
Lipid Particles 0.26 6.59 3.97 2.43 -0.82 4.14 4.89 17.15 10.84 7.05 2.60 7.65 13.35 7.28 4.98
Mitochondria 2.49 5.70 5.75 2.96 2.46 -2.29 6.55 1.49 2.64 -6.58 -0.36 6.98 5.13 3.28 -6.29
Mitotic Spindle -0.06 1.19 1.26 1.28 0.27 -2.45 1.97 2.11 4.31 3.01 -2.40 2.36 2.63 4.54 1.45
None 2.96 4.71 7.53 7.06 6.05 2.10 -0.02 -1.96 -2.46 -1.71 4.33 1.20 -1.14 -2.07 -1.62
Nuclear Periphery -2.30 -1.99 -1.31 1.65 0.94 1.17 -8.70 4.98 3.84 11.76 -1.29 -5.17 1.40 2.93 9.79
Nuclear Periphery Foci -6.96 -8.65 -5.72 -4.66 2.75 3.94 -10.82 9.32 4.45 12.10 -2.93 -13.00 -4.00 -3.33 8.84
Nucleolus -2.08 7.79 8.78 4.89 0.77 3.49 4.62 -3.52 -5.00 -5.64 -0.63 8.77 2.03 1.86 -5.24
Nucleus -1.33 -3.11 5.53 5.19 5.06 10.97 -5.14 -5.79 -7.57 -2.96 4.27 -4.60 -3.63 -5.09 0.04
Peroxisomes -1.38 4.18 2.43 1.94 -1.79 -3.42 7.03 2.56 5.35 -2.36 -2.66 8.29 3.23 4.00 -2.82
Vacuole -0.93 -3.00 -9.48 -9.30 -7.77 -3.88 -19.73 -0.41 1.70 18.12 -3.14 -16.97 -6.76 -5.99 6.53
Vacuole Periphery 3.84 13.84 13.96 9.74 0.64 -4.56 13.48 9.33 9.71 -2.93 -0.64 17.75 14.97 12.48 -2.91
Standard name
Human Ortholog
Description Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ybp1

Ybp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available