Standard name
Human Ortholog
Description Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.96 0.95 0.86 0.79 0.78 – 0.69 0.64 0.65 0.95 0.99 1.0 0.96 0.94 0.97 0.95 0.96 0.94 0.95 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.15 0.14 – 0.22 0.3 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0.1 0.1 – 0.14 0.14 0.17 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 – 0 2 2 0 0 0 0 0 0 0 0 1 0 2 4
Bud 0 0 0 0 1 0 1 4 – 3 5 4 0 0 0 0 1 0 0 2 0 1 13 22
Bud Neck 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 4 1 1 3 10 12 – 10 7 11 0 2 0 0 1 3 0 0 0 0 0 0
Cytoplasm 217 180 262 178 250 266 300 424 – 259 193 303 41 160 180 109 227 143 208 168 250 286 365 395
Endoplasmic Reticulum 2 1 1 1 0 3 4 4 – 12 4 6 0 2 1 5 8 6 2 0 3 2 2 3
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 2 6
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 1 0 0 0 1 3
Mitochondria 0 0 2 6 9 31 55 76 – 83 89 106 0 0 0 3 4 1 3 0 1 3 4 9
Nucleus 0 0 1 1 0 2 2 11 – 6 7 8 1 1 0 11 2 1 0 0 0 0 0 1
Nuclear Periphery 0 0 1 1 0 0 0 2 – 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 1 2 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4
Vac/Vac Membrane 0 0 2 3 9 24 38 53 – 51 43 78 1 0 1 2 13 4 1 1 0 1 3 7
Unique Cell Count 219 181 266 185 264 308 378 543 376 301 464 43 161 180 113 242 147 219 175 265 300 403 466
Labelled Cell Count 221 181 273 191 271 329 411 589 424 351 520 44 165 182 130 258 158 219 175 265 300 403 466


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.6 6.3 6.3 7.8 7.3 7.6 7.7 – 7.8 7.7 8.0 8.8 9.0 10.5 14.0 17.6 22.3 8.8 9.3 9.5
Std Deviation (1e-4) 1.5 2.0 1.5 1.5 2.2 1.9 2.1 2.1 – 2.1 2.2 2.1 2.0 2.0 2.8 5.0 6.7 7.0 4.1 7.6 7.6
Intensity Change (Log2) – – – -0.01 0.31 0.21 0.26 0.29 – 0.31 0.3 0.34 0.48 0.51 0.74 1.16 1.49 1.82 0.49 0.57 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.0 0.7 – 1.0 0.7 0.8 0 0 0 0 0 0
Cytoplasm -1.5 -2.4 -5.3 -7.2 -7.6 – -9.5 -10.3 -10.3 -1.4 0.8 1.7 -1.3 -2.8 -0.9
Endoplasmic Reticulum 0 0 0 0 0 – 2.5 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.1 4.8 6.1 6.0 – 7.9 9.3 8.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 2.1 4.0 4.8 4.8 – 5.8 5.9 6.7 0 0 0 0 3.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7451 6.901 6.4351 5.7561 5.6005 6.5794 4.4552 7.2853 7.4708 6.7311 6.02 6.9776 3.537 6.1681 5.0147 5.1102 5.0092 4.8891
Actin 0.0285 0.0036 0.0235 0.0079 0.008 0.0073 0.019 0.0022 0.0004 0.0003 0.0041 0.0018 0.0309 0.0002 0.0126 0.0002 0.0001 0.0002
Bud 0.0067 0.0166 0.0187 0.0029 0.0003 0.0018 0.0023 0.0069 0.0001 0.0004 0.0001 0.0002 0.001 0.0001 0.0002 0.0001 0.0001 0
Bud Neck 0.012 0.0004 0.0012 0.0014 0.0011 0.0037 0.0005 0.0003 0.0002 0.0005 0.0003 0.0059 0.0004 0.0001 0.0004 0.0002 0.0002 0.0005
Bud Periphery 0.0038 0.0032 0.0074 0.0025 0.0004 0.0008 0.0016 0.0022 0 0.0002 0 0 0.0017 0 0.0001 0 0 0
Bud Site 0.0055 0.0095 0.0047 0.0015 0.0009 0.0009 0.0021 0.0076 0.0003 0.0022 0.0002 0.0001 0.0026 0.0001 0.0056 0 0 0
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0 0 0 0 0.0003 0 0 0 0 0
Cytoplasm 0.6758 0.9012 0.7909 0.7953 0.7254 0.8957 0.8073 0.9045 0.9433 0.8462 0.9243 0.866 0.7853 0.9822 0.9479 0.9867 0.995 0.9692
Cytoplasmic Foci 0.0481 0.0122 0.0408 0.0301 0.0294 0.0087 0.045 0.0388 0.0336 0.09 0.0122 0.0838 0.0122 0.0005 0.002 0.0011 0.0006 0.0168
Eisosomes 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0.0003 0.0011 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0124 0.0023 0.0029 0.009 0.0033 0.0046 0.008 0.0011 0.0007 0.0006 0.0009 0.0006 0.0219 0.0004 0.0009 0.0005 0.0001 0.0008
Endosome 0.0434 0.0047 0.0128 0.0376 0.0658 0.0224 0.0111 0.0004 0.0009 0.03 0.0012 0.0006 0.0118 0.0001 0.0006 0.0004 0.0001 0.0007
Golgi 0.009 0.0005 0.0049 0.0076 0.0298 0.0048 0.0038 0.0002 0.0001 0.0047 0.0029 0.0002 0.004 0 0.0003 0 0 0
Lipid Particles 0.0134 0.0001 0.0054 0.0018 0.0377 0.0034 0.0046 0.0002 0 0.0045 0.0034 0.0005 0.0024 0 0.0001 0 0 0
Mitochondria 0.0145 0.0011 0.0035 0.0034 0.0252 0.0017 0.0169 0.0001 0.0001 0.0019 0.0007 0.0002 0.0172 0 0.0181 0 0 0
None 0.0485 0.0262 0.0285 0.0534 0.0051 0.0137 0.038 0.0286 0.009 0.0029 0.0051 0.024 0.0578 0.0153 0.0079 0.0091 0.0014 0.0071
Nuclear Periphery 0.0257 0.0019 0.0068 0.0049 0.0077 0.01 0.0064 0.0004 0.0004 0.0005 0.0005 0.0008 0.0072 0.0001 0.0005 0.0003 0.0001 0.0008
Nucleolus 0.0021 0.0003 0.0006 0.0002 0.0009 0.0002 0.0004 0.0001 0 0.0005 0 0.0005 0.0003 0 0 0 0 0
Nucleus 0.0202 0.0115 0.0199 0.0089 0.0054 0.0138 0.0029 0.0018 0.0017 0.0031 0.0031 0.0024 0.0022 0.0008 0.0018 0.0011 0.0024 0.0031
Peroxisomes 0.0086 0.0005 0.0162 0.0197 0.0115 0.0008 0.021 0.0022 0.0007 0.0048 0.0404 0.001 0.0047 0 0.0004 0 0 0
Punctate Nuclear 0.0172 0.0023 0.0084 0.003 0.0383 0.0027 0.0072 0.0015 0.008 0.0031 0.0004 0.0107 0.0334 0 0.0002 0.0001 0 0.0005
Vacuole 0.0027 0.0014 0.0019 0.0068 0.0022 0.0018 0.0011 0.0007 0.0002 0.0029 0.0002 0.0002 0.001 0.0001 0.0002 0.0002 0 0.0002
Vacuole Periphery 0.0015 0.0002 0.0005 0.0021 0.0016 0.0009 0.0005 0.0001 0 0.0004 0 0.0001 0.0007 0 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.9752 53.187 56.5096 55.1842 50.4045 55.41 68.291 72.5921 65.7736 60.9989
Translational Efficiency 1.3804 1.5761 1.4099 1.4748 1.4764 1.4961 1.4561 1.5247 1.4748 1.2657

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 370 330 1376 221 1336 2174 958 310 1706 2504 2334

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3041.05 941.99 1320.47 1384.38 752.37 866.52 1196.24 1414.83 1409.44 882.89 1212.61 1396.88
Standard Deviation 1272.95 166.97 224.13 289.43 92.45 137.17 239.71 363.54 1242.33 147.48 241.40 322.27
Intensity Change Log 2 — -1.690786 -1.203518 -1.135330 — 0.203791 0.668993 0.911114 — -1.068698 -0.591960 -0.438479

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001822 0.000235 0.000343 0.001428 0.000144 0.000216 0.000112 0.002496 0.000626 0.000220 0.000142 0.001866
Bud Neck 0.022453 0.003231 0.001470 0.004240 0.009981 0.005137 0.003296 0.001787 0.013561 0.004724 0.003056 0.003233
Bud Site 0.229101* 0.010068 0.003935 0.028618 0.005458 0.009909 0.006430 0.018763 0.069666 0.009943 0.006101 0.024573
Cell Periphery 0.001972 0.000086 0.000155 0.000165 0.000198 0.000115 0.000046 0.000120 0.000707 0.000109 0.000061 0.000146
Cytoplasm 0.127031* 0.724598* 0.708916* 0.608128* 0.505080* 0.671576* 0.817412* 0.790701* 0.396544* 0.683075* 0.803113* 0.683065*
Cytoplasmic Foci 0.489785* 0.102832 0.017856 0.028141 0.280579* 0.140067 0.008508 0.017796 0.340641* 0.131992 0.009740 0.023895
Eisosomes 0.000195 0.000032 0.000009 0.000017 0.000184 0.000051 0.000006 0.000011 0.000187 0.000047 0.000006 0.000015
Endoplasmic Reticulum 0.008740 0.007029 0.000730 0.001719 0.000666 0.000953 0.000223 0.000243 0.002984 0.002271 0.000290 0.001113
Endosome 0.002471 0.008161 0.001043 0.018281 0.022654 0.019116 0.001036 0.001796 0.016860 0.016740 0.001036 0.011515
Golgi 0.001047 0.000563 0.000229 0.018761 0.001492 0.003278 0.000153 0.002522 0.001364 0.002689 0.000163 0.012095
Lipid Particles 0.006786 0.001223 0.000931 0.002721 0.007005 0.002639 0.000295 0.000805 0.006942 0.002332 0.000379 0.001934
Mitochondria 0.000590 0.000173 0.000155 0.006167 0.000701 0.002699 0.000222 0.000247 0.000669 0.002151 0.000213 0.003737
Mitotic Spindle 0.012142 0.001591 0.003827 0.099875 0.000076 0.005610 0.014794 0.029442 0.003540 0.004738 0.013349 0.070965
None 0.000449 0.001890 0.007591 0.002732 0.001117 0.002591 0.007313 0.006820 0.000925 0.002439 0.007350 0.004410
Nuclear Periphery 0.000945 0.000247 0.000426 0.001296 0.000369 0.000203 0.000237 0.000159 0.000535 0.000212 0.000262 0.000829
Nuclear Periphery Foci 0.001895 0.002240 0.003780 0.005151 0.000933 0.001190 0.000873 0.002605 0.001209 0.001418 0.001256 0.004106
Nucleolus 0.001194 0.000332 0.000107 0.000487 0.000474 0.000574 0.000153 0.000293 0.000681 0.000522 0.000147 0.000407
Nucleus 0.009898 0.056810 0.205864* 0.063537 0.069640 0.038308 0.089206 0.075531 0.052489 0.042320 0.104580 0.068460
Peroxisomes 0.005096 0.002203 0.000077 0.004778 0.004995 0.002162 0.000392 0.001745 0.005024 0.002171 0.000351 0.003533
Vacuole 0.074108 0.075968 0.042470 0.095421 0.087369 0.092048 0.049054 0.045607 0.083562 0.088560 0.048186 0.074975
Vacuole Periphery 0.002280 0.000487 0.000086 0.008338 0.000885 0.001561 0.000239 0.000511 0.001286 0.001328 0.000219 0.005125

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.05 1.76 1.59 -3.79 -1.26 -1.43 0.51 -1.65 -1.49 -1.69 1.83 1.99 -0.10 -2.72 -2.96
Bud Neck 4.73 5.12 4.96 0.69 -1.75 3.09 4.75 5.46 5.84 1.47 5.27 6.76 7.05 4.81 0.09
Bud Site 6.33 6.50 6.12 -2.46 -5.63 -3.14 0.04 -3.35 -0.68 -3.71 5.04 5.61 4.61 -2.49 -6.66
Cell Periphery 3.87 3.59 3.77 -4.11 3.96 4.40 7.71 2.49 -2.50 -7.78 4.09 4.29 3.88 -3.44 -7.85
Cytoplasm -12.11 -10.32 -9.52 7.22 2.95 -8.52 -13.12 -10.69 -4.09 4.28 -13.18 -16.86 -11.57 4.03 13.06
Cytoplasmic Foci 5.09 7.36 7.52 11.50 2.51 9.70 19.42 18.66 26.85 -6.49 10.26 18.93 18.49 29.93 -6.29
Eisosomes 1.58 1.93 1.94 5.09 0.41 8.75 11.73 11.52 12.98 -2.96 6.43 8.57 8.37 13.76 -5.71
Endoplasmic Reticulum -0.54 0.79 0.93 1.59 2.86 -1.36 3.15 3.69 4.69 2.51 -0.47 1.22 1.18 2.15 -0.61
Endosome -4.05 0.57 -2.40 2.55 -3.38 1.26 7.87 7.88 14.68 0.20 0.12 7.66 6.76 12.83 -3.98
Golgi 1.44 2.63 -2.38 -2.78 -3.05 -2.28 4.23 1.79 3.35 -1.60 -2.25 4.86 -1.37 0.56 -3.43
Lipid Particles 2.25 2.18 2.02 -3.64 -3.10 2.76 4.19 3.65 3.96 -6.92 3.42 4.40 3.70 1.64 -10.49
Mitochondria 2.35 2.08 -1.41 -2.47 -2.36 -2.84 2.20 2.38 3.83 1.08 -2.71 2.71 -0.35 2.39 -2.29
Mitotic Spindle 2.55 2.04 0.01 -8.66 -6.03 -3.61 -8.82 -5.81 -2.94 0.86 1.20 -1.62 -3.71 -7.74 -3.26
None -2.95 -9.33 -8.40 -4.98 5.63 -1.84 -7.67 -6.42 -3.90 1.42 -2.24 -9.98 -7.08 -3.33 5.29
Nuclear Periphery 1.83 0.34 -0.06 -7.88 -1.49 1.68 -0.99 0.16 -4.86 4.37 2.51 0.02 -1.23 -9.71 -3.46
Nuclear Periphery Foci 0.04 -3.32 -1.44 -1.82 2.79 -1.04 -1.14 -6.51 -6.74 -6.87 -0.40 -1.73 -6.35 -7.54 -5.63
Nucleolus 0.98 1.57 1.32 1.72 -3.32 -0.79 4.78 4.23 3.33 0.50 0.17 3.09 2.83 3.41 -0.96
Nucleus -3.77 -16.95 -9.99 -6.08 12.91 5.16 -6.60 -3.10 -15.00 5.46 3.37 -12.86 -6.19 -18.55 11.30
Peroxisomes 0.69 3.58 2.19 1.80 -2.52 2.35 4.19 3.87 4.44 -1.09 2.45 4.95 4.12 4.05 -2.36
Vacuole 2.42 2.76 -2.24 -13.12 -13.03 -0.62 -0.60 -4.93 -6.09 -6.17 1.57 1.74 -7.41 -16.89 -17.24
Vacuole Periphery 0.90 1.38 -0.42 -2.54 -3.51 -2.15 2.91 2.80 6.99 -0.11 -1.12 2.90 0.05 1.33 -3.31
Standard name
Human Ortholog
Description Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase
Localization
Cell Percentages cytoplasm (50%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pcs60

Pcs60


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pcs60-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available