Standard name
Human Ortholog
Description Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0.06 0.08 0.14 0.16 0.17 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.38 0 0 0.29 0.17 0.16 0.09 0.45 0.27 0.33 0.33 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.91 0.86 0.94 0.94 0.87 0.82 0.88 0.87 0.76 0.81 0.84 0.84 0.92 0.87 0.82 0.72 0.69 0.72 0.84 0.83 0.78 0.66 0.67 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.23 0 0 0.07 0 0.24 0.09 0.07 0.26 0.25 0.16 0.15 0.05 0.09 0.08 0 0.06 0.06 0.08 0.05 0.1 0.16 0.19 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0.05 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud 0 0 0 0 0 1 1 0 1 1 4 4 0 0 0 0 1 0 1 2 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0
Bud Site 0 1 0 0 3 1 2 2 11 7 7 25 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 14 6 2 7 4 7 11 6 7 3 9 12 27 46 37 30 35 0 5 0 2 5 1
Endoplasmic Reticulum 0 3 0 0 0 0 0 0 0 0 0 0 1 0 1 5 3 9 0 0 1 0 1 0
Endosome 0 0 3 0 0 0 0 2 0 0 0 0 0 0 4 7 7 5 1 3 3 4 3 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 1 0 0 1
Mitochondria 9 154 1 4 28 19 26 20 103 58 90 96 1 7 2 15 8 5 1 6 2 1 1 2
Nucleus 143 346 155 84 83 91 140 192 174 175 232 245 198 280 279 165 125 168 141 334 140 80 166 145
Nuclear Periphery 0 0 0 0 0 0 1 1 3 1 2 1 1 2 0 2 1 1 0 0 0 0 2 1
Nucleolus 37 18 3 6 4 27 14 15 59 55 45 45 10 29 26 9 10 13 14 21 18 20 46 47
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 1 4 3 3 0 1 2 5 0 2 3 4 2 14 5 0 5 4 3 3 1 3 4 4
Vac/Vac Membrane 5 1 1 0 0 0 1 2 0 0 1 1 0 1 6 14 13 14 2 15 7 5 9 16
Unique Cell Count 158 402 165 89 95 111 159 221 229 217 275 293 215 322 340 228 180 233 169 403 180 122 249 237
Labelled Cell Count 197 541 173 99 125 144 194 251 358 306 387 430 225 362 371 254 204 255 169 403 180 122 249 237


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 4.6 6.3 6.0 4.3 4.8 5.1 5.5 4.4 4.8 4.8 4.7 6.5 6.1 6.1 6.4 7.7 7.9 5.9 7.6 7.6
Std Deviation (1e-4) 1.1 0.8 1.1 1.0 1.1 1.0 0.9 1.3 1.0 1.1 0.9 1.0 1.3 1.2 1.5 1.8 1.9 2.3 1.3 1.9 1.8
Intensity Change (Log2) – – – -0.08 -0.54 -0.39 -0.32 -0.21 -0.54 -0.4 -0.41 -0.42 0.03 -0.04 -0.05 0.02 0.28 0.32 -0.09 0.26 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 3.1 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9336 5.2754 4.8898 5.4918 5.1206 5.2834 5.9656 5.5016 5.7471 5.5628 6.0262 5.7742 3.0117 3.115 2.7669 2.8623 2.9311 3.058
Actin 0.0186 0 0.0007 0 0.0041 0.0014 0.0023 0.0169 0.0031 0.02 0.0093 0.002 0.0186 0.0004 0.0072 0 0.004 0.0044
Bud 0.0007 0 0.0003 0 0.0002 0.0001 0.0003 0.0002 0.0003 0.0001 0.0015 0.0001 0.0009 0.0001 0.0001 0 0.0009 0.0002
Bud Neck 0.0032 0.0001 0.0004 0.0001 0.0006 0.0023 0.0012 0.0004 0.0053 0.0035 0.0025 0.0009 0.0026 0.0001 0.0035 0 0.0017 0.002
Bud Periphery 0.0017 0 0.0018 0 0.0004 0.0006 0.0008 0.0001 0.0007 0.0001 0.0048 0.0003 0.0021 0.0001 0.0004 0 0.0029 0.0009
Bud Site 0.0058 0.0001 0.0006 0 0.0014 0.0004 0.0012 0.0112 0.0018 0.0036 0.0036 0.0002 0.0032 0.0004 0.0017 0 0.002 0.0002
Cell Periphery 0.0006 0 0.0003 0 0.0001 0.0002 0.0003 0.0001 0.0001 0.0005 0.001 0 0.0006 0 0.0004 0 0.0003 0.0001
Cytoplasm 0.0043 0 0.0002 0.0001 0.0014 0.0001 0.0034 0.0002 0.0007 0.0093 0.0078 0.0004 0.0073 0.0001 0.0063 0 0.0298 0.0001
Cytoplasmic Foci 0.0073 0 0.0017 0 0.0103 0.0005 0.001 0.0005 0.0039 0.0052 0.0051 0.0008 0.0125 0.0001 0.0082 0 0.0078 0.0009
Eisosomes 0.0003 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0003 0 0.0002 0 0.0003 0 0.0001 0.0001
Endoplasmic Reticulum 0.0012 0 0.0001 0 0.0011 0.0002 0.006 0 0.0015 0.0033 0.0025 0.0007 0.0029 0 0.003 0 0.004 0.0002
Endosome 0.0133 0 0.0012 0 0.0181 0.0019 0.0078 0.0001 0.0049 0.0012 0.0169 0.0034 0.0097 0.0004 0.004 0 0.0197 0.0034
Golgi 0.0048 0 0.0005 0 0.0038 0.0013 0.0022 0.0004 0.0024 0.0021 0.0029 0.001 0.0039 0.0001 0.0022 0 0.0062 0.0027
Lipid Particles 0.0105 0 0.0024 0 0.0172 0.0009 0.0013 0.0001 0.0052 0.0022 0.0021 0.0019 0.0148 0 0.0067 0 0.0146 0.0063
Mitochondria 0.009 0.0001 0.0025 0.0001 0.0033 0.0117 0.0022 0.0001 0.0026 0.0003 0.0121 0.0009 0.0104 0.0114 0.0022 0.0001 0.018 0.0047
None 0.0015 0 0.0002 0.0007 0.0018 0.0001 0.0006 0 0.0002 0.0006 0.0359 0.0001 0.0034 0.0001 0.0014 0 0.0036 0.0002
Nuclear Periphery 0.0105 0.0002 0.0065 0.0004 0.0472 0.0005 0.0109 0.0003 0.0032 0.0031 0.0117 0.0063 0.0193 0.0006 0.0018 0.0004 0.0172 0.0015
Nucleolus 0.0378 0.0229 0.024 0.0164 0.0764 0.0902 0.0232 0.0345 0.0149 0.0059 0.0598 0.0605 0.0281 0.023 0.0211 0.0114 0.0898 0.0653
Nucleus 0.8346 0.9757 0.9519 0.9801 0.7867 0.8827 0.9255 0.9305 0.9426 0.9144 0.7952 0.9162 0.8199 0.9613 0.9208 0.9875 0.7272 0.8998
Peroxisomes 0.0128 0 0.0022 0 0.0151 0.0011 0.0002 0.0002 0.0024 0.0231 0.0021 0.0005 0.0206 0.0002 0.0045 0 0.0238 0.0013
Punctate Nuclear 0.0135 0.0008 0.0009 0.0022 0.0083 0.0027 0.0032 0.004 0.0027 0.0012 0.0175 0.0032 0.0121 0.0013 0.0034 0.0003 0.0201 0.0038
Vacuole 0.0056 0 0.0012 0 0.0009 0.0006 0.0044 0.0001 0.0008 0.0001 0.0038 0.0003 0.005 0.0001 0.0005 0 0.0041 0.0009
Vacuole Periphery 0.0024 0 0.0004 0 0.0017 0.0003 0.0019 0 0.0005 0 0.0016 0.0003 0.0016 0.0001 0.0002 0 0.0024 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2592 8.6524 11.2358 13.5327 15.6865 7.2552 12.5759 11.0712 22.9236 13.3148
Translational Efficiency 3.7549 2.6299 2.3325 2.8697 2.058 4.3065 1.9103 3.6207 1.4651 1.7929

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1187 986 1580 1321 1699 2067 313 1258 2886 3053 1893 2579

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 807.56 834.51 910.85 949.60 720.90 934.20 1054.56 956.95 756.54 902.00 934.61 953.19
Standard Deviation 124.83 158.77 120.94 157.29 131.59 126.95 122.77 145.50 135.72 145.69 132.48 151.70
Intensity Change Log 2 — 0.047360 0.173644 0.233750 — 0.373932 0.548770 0.408644 — 0.210619 0.362752 0.318886

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000532 0.001154 0.001064 0.001628 0.000326 0.000443 0.001966 0.003521 0.000411 0.000673 0.001213 0.002552
Bud Neck 0.002605 0.013601 0.025989 0.027958 0.005139 0.014410 0.018400 0.037414 0.004097 0.014148 0.024734 0.032570
Bud Site 0.000389 0.003575 0.002414 0.022744 0.000912 0.002672 0.002363 0.007957 0.000697 0.002964 0.002405 0.015531
Cell Periphery 0.000070 0.000290 0.000062 0.000261 0.000137 0.000108 0.000073 0.000309 0.000110 0.000167 0.000064 0.000284
Cytoplasm 0.003139 0.013936 0.001504 0.040520 0.006530 0.004281 0.000876 0.013858 0.005135 0.007399 0.001400 0.027514
Cytoplasmic Foci 0.002590 0.002014 0.000075 0.008053 0.002076 0.000918 0.000048 0.001047 0.002287 0.001272 0.000071 0.004636
Eisosomes 0.000031 0.000039 0.000040 0.000043 0.000024 0.000026 0.000064 0.000085 0.000027 0.000030 0.000044 0.000064
Endoplasmic Reticulum 0.003087 0.004807 0.004223 0.006912 0.002433 0.002968 0.006847 0.006730 0.002702 0.003562 0.004657 0.006823
Endosome 0.000633 0.001241 0.000502 0.014121 0.000384 0.001048 0.000245 0.002207 0.000486 0.001110 0.000460 0.008310
Golgi 0.000378 0.000762 0.000213 0.012635 0.000189 0.000278 0.000196 0.000840 0.000267 0.000434 0.000210 0.006881
Lipid Particles 0.001234 0.000668 0.000149 0.001196 0.000328 0.000426 0.000221 0.000638 0.000700 0.000504 0.000161 0.000924
Mitochondria 0.002966 0.004656 0.001845 0.011025 0.000834 0.003529 0.001553 0.005116 0.001711 0.003893 0.001797 0.008143
Mitotic Spindle 0.003121 0.002865 0.003632 0.082591 0.000932 0.006629 0.002551 0.025260 0.001832 0.005413 0.003454 0.054625
None 0.008715 0.022415 0.002404 0.013726 0.019914 0.004104 0.002368 0.007632 0.015308 0.010017 0.002398 0.010753
Nuclear Periphery 0.000289 0.000574 0.000505 0.005671 0.000424 0.001015 0.000398 0.001301 0.000368 0.000872 0.000487 0.003539
Nuclear Periphery Foci 0.001249 0.000855 0.000280 0.005521 0.000760 0.001201 0.000211 0.000743 0.000961 0.001089 0.000269 0.003190
Nucleolus 0.065270 0.075019 0.057390 0.017565 0.058956 0.060370 0.049839 0.029926 0.061553 0.065101 0.056142 0.023594
Nucleus 0.901254* 0.847942* 0.895098* 0.681255* 0.898419* 0.891911* 0.907724* 0.846108* 0.899585* 0.877711* 0.897186* 0.761668*
Peroxisomes 0.001150 0.001552 0.000510 0.001686 0.000438 0.000727 0.001151 0.001384 0.000731 0.000993 0.000616 0.001539
Vacuole 0.000732 0.001319 0.001331 0.033132 0.000695 0.001993 0.002690 0.005960 0.000710 0.001776 0.001556 0.019878
Vacuole Periphery 0.000566 0.000716 0.000768 0.011757 0.000151 0.000942 0.000215 0.001964 0.000322 0.000869 0.000677 0.006980

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.32 -11.22 -15.27 -7.91 -9.19 -2.80 -15.27 -4.81 -4.58 -0.44 -5.41 -20.98 -8.30 -7.24 -4.02
Bud Neck -11.00 -19.87 -20.20 -11.84 -2.98 -9.67 -11.63 -20.27 -14.29 -6.66 -13.97 -21.26 -28.22 -18.23 -6.46
Bud Site -4.01 -9.72 -9.06 -6.52 -7.52 -3.12 -8.01 -7.47 -5.29 -4.44 -5.02 -8.97 -11.44 -8.57 -8.99
Cell Periphery -1.87 0.46 -9.48 0.74 -11.83 0.76 2.14 -1.69 -2.01 -2.81 -1.16 2.98 -3.74 -0.98 -5.85
Cytoplasm -4.35 1.69 -9.99 -3.82 -11.25 1.52 5.41 -3.09 -4.46 -7.64 -2.09 5.02 -9.53 -7.15 -13.27
Cytoplasmic Foci 0.64 3.11 0.18 -0.63 -4.79 2.07 3.91 2.77 1.44 -2.98 2.05 4.92 1.49 -0.72 -5.45
Eisosomes -2.86 -6.21 -11.26 -8.03 -6.48 -1.24 -13.13 -2.36 -2.26 0.29 -2.21 -13.55 -3.89 -3.58 -1.56
Endoplasmic Reticulum -4.57 -6.45 -11.98 -7.93 -8.67 -2.72 -10.20 -15.60 -13.92 0.92 -4.60 -13.15 -19.17 -15.96 -9.08
Endosome -1.44 0.67 -5.31 -4.35 -5.64 -3.53 -0.02 -3.37 -1.40 -3.41 -3.24 0.15 -6.29 -4.75 -6.34
Golgi -1.78 1.35 -3.23 -2.84 -3.36 -1.63 -2.27 -2.50 -1.93 -1.94 -2.05 0.28 -3.57 -3.26 -3.61
Lipid Particles 1.57 3.52 1.39 -0.58 -5.48 -0.88 0.50 -1.49 -0.91 -1.79 1.41 3.93 0.21 -1.52 -5.43
Mitochondria -1.06 0.96 -2.75 -1.14 -5.77 -4.64 -9.62 -5.63 -1.12 -3.38 -3.16 -0.90 -5.87 -2.05 -6.70
Mitotic Spindle 0.09 -2.07 -11.79 -12.07 -11.08 -3.84 -1.77 -8.70 -6.98 -5.91 -3.07 -3.80 -14.77 -13.31 -12.63
None -4.27 5.06 -0.17 4.34 -7.09 7.27 7.95 6.00 -2.45 -4.12 3.22 9.41 5.17 1.93 -8.38
Nuclear Periphery -1.68 -6.49 -8.43 -7.40 -7.61 -3.29 -3.12 -10.33 -4.85 -7.13 -3.82 -5.65 -11.11 -8.30 -9.83
Nuclear Periphery Foci 1.01 2.00 -1.94 -2.98 -3.69 -0.96 2.69 -0.50 0.68 -3.51 -0.23 2.63 -2.22 -1.87 -4.23
Nucleolus -2.19 0.33 13.47 14.73 13.34 -0.86 2.07 7.95 9.06 2.88 -1.69 0.03 15.25 17.01 12.25
Nucleus 6.71 3.67 15.91 9.15 14.17 1.41 1.34 10.31 9.42 7.65 5.01 4.17 18.77 14.65 15.37
Peroxisomes -0.93 2.53 1.26 2.29 -2.19 -2.56 -5.80 -1.37 -0.60 0.55 -1.55 0.61 -0.70 0.37 -1.15
Vacuole -1.94 -3.12 -11.24 -11.01 -10.92 -2.66 -3.05 -7.06 -6.27 -3.69 -3.16 -4.36 -13.19 -12.45 -12.19
Vacuole Periphery -0.46 -0.08 -3.90 -3.92 -3.83 -4.31 -5.45 -2.76 -0.58 -2.45 -3.06 -0.77 -4.85 -3.62 -3.89
Standard name
Human Ortholog
Description Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Swc5

Swc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available