Standard name
Human Ortholog
Description Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.13 0.17 0.2 0.08 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.06 0 0 0.08 0.19 0.25 0.14 0.2 0.28 0.3 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.92 0.93 0.92 0.86 0.82 0.75 0.74 0.77 0.72 0.71 0.71 0.86 0.79 0.76 0.88 0.88 0.85 0.88 0.9 0.87 0.86 0.71 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.1 0.07 0 0.09 0.24 0.24 0.26 0.25 0.29 0.3 0.27 0 0 0 0 0 0 0.05 0 0 0.05 0.09 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.13 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 2 1
Bud 0 0 0 0 3 3 10 3 10 14 5 5 0 1 0 1 0 1 0 2 7 2 2 6
Bud Neck 0 0 0 0 0 1 0 0 1 4 1 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 2 2 2 6 11 9 7 19 29 23 32 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 11 25 24 39 16 21 29 10 9 10 11 30 66 84 42 47 30 0 2 2 3 3 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 6 1 7 0 0 0 0 0 0
Endosome 1 0 4 1 2 1 0 1 0 0 0 1 1 4 4 1 1 1 0 1 7 1 8 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 3 2 2 1
Mitochondria 22 25 22 25 58 204 307 153 202 361 278 312 0 0 0 4 11 4 0 3 3 2 4 10
Nucleus 127 390 764 582 655 880 933 813 784 910 667 725 191 305 316 445 416 260 122 390 756 312 349 315
Nuclear Periphery 4 1 4 10 3 4 12 12 30 16 12 10 0 2 1 4 4 1 0 0 3 0 3 3
Nucleolus 28 42 55 27 72 257 291 288 254 363 278 270 1 3 5 14 10 6 7 9 29 18 45 46
Peroxisomes 0 0 1 1 1 0 0 1 0 2 0 0 1 0 7 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 5 2 2 0 0 6 5 5 2 6 0 5 6 0 3 0 0 3 5 1 3 7
Vac/Vac Membrane 0 2 5 4 5 4 13 7 3 9 2 4 2 9 8 8 3 6 2 14 37 16 63 55
Unique Cell Count 140 423 825 636 765 1077 1238 1098 1017 1269 938 1014 223 388 418 507 475 305 140 432 867 364 495 460
Labelled Cell Count 182 473 887 678 847 1381 1596 1320 1318 1723 1278 1380 228 395 432 526 496 317 140 432 867 364 495 460


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.0 6.7 6.3 6.2 5.3 5.4 5.6 5.2 5.1 4.8 5.0 9.0 8.7 8.2 10.1 9.1 9.8 9.6 10.2 10.3
Std Deviation (1e-4) 1.0 1.3 1.3 1.6 1.5 1.2 1.4 1.5 1.2 1.2 1.3 1.3 2.5 2.4 2.9 2.9 2.3 2.1 2.0 2.5 2.2
Intensity Change (Log2) – – – -0.08 -0.11 -0.33 -0.31 -0.25 -0.36 -0.4 -0.48 -0.41 0.44 0.39 0.3 0.59 0.45 0.56 0.53 0.61 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3.0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.0 0 0 3.3 3.8 0 4.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 2.1 -2.3 -2.0 -0.5 -3.2 -4.1 -3.0 -3.0 6.2 8.6 10.0 4.3 5.2 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 4.5 10.9 13.5 8.5 11.2 14.9 0 15.5 0 -3.2 -3.4 -2.4 -0.4 -1.3
Nucleus -0.8 -4.5 -6.9 -10.0 -10.5 -9.0 -11.7 -11.5 -11.5 -3.2 -7.0 -8.4 -3.0 -3.0 -3.8
Nuclear Periphery 2.1 0 0 1.2 1.5 3.9 1.8 0 1.2 0 0 0 0 0 0
Nucleolus -2.0 2.0 10.0 10.0 11.1 10.5 12.3 0 11.2 -3.7 -4.5 -4.3 -3.1 -3.6 -3.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.1 0.1 0 0.3 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9265 8.3979 8.3662 7.3751 7.4214 7.8843 6.2918 6.94 6.4412 5.7937 5.3933 5.9912 9.7331 10.0715 9.7493 8.9257 8.8701 9.9047
Actin 0.0196 0 0.0014 0 0.0002 0.0022 0.073 0 0.0041 0.0684 0.0008 0.0094 0.0006 0 0.0008 0 0 0.0001
Bud 0.0003 0 0.0001 0 0.0001 0.0001 0.0015 0 0.0001 0.0001 0.0002 0.0008 0.0004 0 0.0001 0.0002 0.0001 0
Bud Neck 0.0044 0.0001 0.0001 0 0.0134 0.0006 0.0033 0 0.0021 0.0002 0.0004 0.001 0.0004 0 0.0001 0 0.0003 0.0002
Bud Periphery 0.0006 0 0.0001 0 0.0006 0.0002 0.0031 0 0.0001 0.0001 0.001 0.0008 0.0002 0 0 0.0001 0.0001 0
Bud Site 0.0039 0.0001 0.0001 0 0.0002 0.0002 0.0064 0 0.0117 0.0003 0.0007 0.0029 0.0003 0 0 0.0001 0.0003 0
Cell Periphery 0.0007 0 0.0001 0 0.0003 0 0.0006 0 0.0002 0 0.0007 0.0002 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0117 0.0016 0.0002 0 0.0007 0.0007 0.0041 0 0.003 0.0005 0.0006 0.0014 0.0021 0.0004 0.0009 0.0001 0.0006 0.0001
Cytoplasmic Foci 0.0039 0 0.0006 0 0 0.0002 0.0192 0 0.0039 0.0119 0.0012 0.0034 0.0005 0.0001 0.0014 0 0.0002 0.0001
Eisosomes 0.0005 0 0 0 0 0 0.0004 0 0.0001 0.0001 0.0001 0.0002 0 0 0 0 0 0
Endoplasmic Reticulum 0.0031 0.0001 0.0004 0 0.0005 0.0006 0.0052 0 0.0012 0.0003 0.0019 0.0021 0.0005 0 0.0013 0 0.0001 0
Endosome 0.0086 0.0001 0.0016 0 0.0006 0.0011 0.0389 0 0.0009 0.0037 0.0025 0.0102 0.0014 0 0.0014 0 0.0002 0
Golgi 0.0015 0 0.0005 0 0 0.0002 0.0139 0 0.0007 0.009 0.0007 0.0053 0.0001 0 0.0001 0 0 0
Lipid Particles 0.0032 0 0.0018 0 0.0001 0.0001 0.0198 0 0.0023 0.0061 0.0102 0.0047 0.0007 0 0.0004 0 0.0001 0
Mitochondria 0.0019 0.0001 0.0007 0 0.0005 0.0004 0.0152 0.0001 0.0005 0.0009 0.0359 0.0066 0.0077 0 0.0031 0.0055 0.0002 0.0001
None 0.0135 0.0001 0.0003 0 0.0001 0.0001 0.0034 0 0.0017 0.0003 0.0009 0.0009 0.0034 0 0.0015 0.0001 0.0002 0
Nuclear Periphery 0.004 0.0045 0.0058 0.0003 0.0081 0.0033 0.0326 0.0006 0.0035 0.0004 0.0278 0.0066 0.0076 0.0014 0.0037 0.0004 0.0043 0.0007
Nucleolus 0.0156 0.0131 0.0143 0.0067 0.0863 0.0487 0.0399 0.0129 0.0101 0.0043 0.0368 0.0407 0.0335 0.0141 0.0209 0.027 0.0561 0.0435
Nucleus 0.8898 0.9614 0.9671 0.9928 0.8686 0.9388 0.6654 0.9859 0.9458 0.8776 0.8547 0.8954 0.9224 0.962 0.9498 0.9456 0.9205 0.9342
Peroxisomes 0.0062 0 0.0005 0 0 0 0.0102 0 0.0021 0.0152 0.0005 0.0022 0.0002 0 0.0002 0.0001 0 0
Punctate Nuclear 0.0017 0.0177 0.0039 0.0001 0.0158 0.002 0.0279 0.0003 0.0053 0.0006 0.0088 0.0034 0.0165 0.0217 0.0133 0.02 0.0161 0.0208
Vacuole 0.0046 0.0008 0.0002 0 0.0031 0.0003 0.0081 0 0.0004 0 0.0044 0.0009 0.0008 0.0001 0.0003 0.0003 0.0004 0
Vacuole Periphery 0.0007 0.0002 0.0003 0 0.0008 0.0001 0.008 0 0.0003 0 0.009 0.0008 0.0007 0 0.0006 0.0005 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.0413 31.6002 29.0317 32.4388 22.916 25.2356 32.3312 34.4008 36.1819 30.2038
Translational Efficiency 1.5546 1.032 1.2353 1.1137 1.3517 1.4169 1.1031 1.119 1.0392 1.1181

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1842 735 1545 1029 1669 1688 1851 2561 3511 2423 3396 3590

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 847.87 922.18 973.99 1159.84 824.54 905.39 945.92 1139.46 836.78 910.48 958.69 1145.30
Standard Deviation 121.77 170.16 165.68 184.89 122.41 148.70 176.12 204.98 122.63 155.72 172.02 199.64
Intensity Change Log 2 — 0.121205 0.200064 0.452011 — 0.134950 0.198129 0.466689 — 0.127998 0.199110 0.459266

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000357 0.000854 0.000810 0.000979 0.000302 0.000695 0.000630 0.001013 0.000331 0.000743 0.000712 0.001003
Bud Neck 0.008445 0.013414 0.020049 0.022014 0.009858 0.015708 0.015087 0.050499 0.009116 0.015012 0.017344 0.042335
Bud Site 0.000682 0.001991 0.001397 0.002213 0.001280 0.001817 0.002109 0.008886 0.000966 0.001870 0.001785 0.006973
Cell Periphery 0.000070 0.000097 0.000064 0.000155 0.000193 0.000132 0.001221 0.000314 0.000129 0.000121 0.000695 0.000269
Cytoplasm 0.000287 0.001012 0.000829 0.000981 0.000810 0.000772 0.016443 0.007090 0.000536 0.000845 0.009339 0.005339
Cytoplasmic Foci 0.000078 0.000747 0.000026 0.000409 0.000378 0.001084 0.003057 0.001122 0.000221 0.000982 0.001678 0.000918
Eisosomes 0.000033 0.000039 0.000051 0.000041 0.000029 0.000072 0.000165 0.000035 0.000031 0.000062 0.000113 0.000036
Endoplasmic Reticulum 0.003540 0.003265 0.002361 0.002563 0.003975 0.004497 0.002515 0.003825 0.003747 0.004123 0.002445 0.003464
Endosome 0.000220 0.000591 0.000106 0.001028 0.000298 0.002170 0.000504 0.002030 0.000257 0.001691 0.000323 0.001743
Golgi 0.000083 0.001053 0.000081 0.000461 0.000212 0.000745 0.000263 0.000689 0.000145 0.000839 0.000180 0.000624
Lipid Particles 0.000078 0.000133 0.000044 0.000287 0.000193 0.001113 0.000244 0.000188 0.000133 0.000815 0.000153 0.000216
Mitochondria 0.000696 0.001327 0.001633 0.003662 0.000943 0.003904 0.001628 0.007029 0.000814 0.003122 0.001630 0.006064
Mitotic Spindle 0.001523 0.002162 0.001434 0.006056 0.000889 0.005150 0.003012 0.020277 0.001221 0.004243 0.002294 0.016201
None 0.001148 0.003122 0.001435 0.002096 0.002585 0.001434 0.029448 0.005481 0.001831 0.001946 0.016704 0.004511
Nuclear Periphery 0.000221 0.000197 0.000214 0.001060 0.000305 0.000571 0.000316 0.003659 0.000261 0.000457 0.000269 0.002914
Nuclear Periphery Foci 0.000134 0.000899 0.000063 0.000554 0.000259 0.000827 0.000486 0.001316 0.000194 0.000849 0.000293 0.001097
Nucleolus 0.027101 0.033894 0.032092 0.010473 0.020443 0.035869 0.019149 0.008017 0.023936 0.035270 0.025037 0.008721
Nucleus 0.954445* 0.933301* 0.936188* 0.939009* 0.956131* 0.918458* 0.901092* 0.868881* 0.955246* 0.922960* 0.917059* 0.888982*
Peroxisomes 0.000278 0.000969 0.000523 0.000323 0.000232 0.001078 0.000603 0.000208 0.000256 0.001045 0.000566 0.000241
Vacuole 0.000402 0.000492 0.000436 0.002900 0.000471 0.002604 0.001628 0.007006 0.000435 0.001964 0.001086 0.005829
Vacuole Periphery 0.000177 0.000442 0.000166 0.002737 0.000214 0.001300 0.000400 0.002434 0.000195 0.001040 0.000293 0.002521

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.57 -10.91 -9.80 -1.78 -2.40 -7.78 -11.64 -9.43 -4.49 -4.56 -8.51 -15.52 -11.89 -4.74 -4.64
Bud Neck -3.57 -11.66 -10.49 -5.80 -1.91 -4.28 -5.34 -18.10 -15.01 -14.94 -6.17 -11.70 -21.19 -16.05 -14.23
Bud Site -2.86 -4.28 -3.61 -0.53 -1.56 -1.10 -1.34 -8.73 -8.11 -6.82 -2.88 -2.68 -10.00 -7.86 -7.64
Cell Periphery -1.50 0.35 -1.67 -0.97 -1.85 1.09 -4.07 -0.83 -2.85 3.92 0.25 -4.14 -2.45 -3.04 3.42
Cytoplasm -2.20 -1.66 -2.94 -0.58 -1.39 0.17 -9.31 -8.36 -8.12 4.54 -1.09 -9.44 -9.28 -7.98 2.83
Cytoplasmic Foci -1.63 1.61 -1.69 1.05 -2.17 -1.62 -4.20 -0.80 0.85 3.59 -2.69 -4.33 -1.57 1.17 2.61
Eisosomes -2.68 -10.95 -4.78 -1.71 4.48 -2.20 -6.16 -2.52 1.93 5.90 -2.28 -6.99 -3.99 1.84 6.43
Endoplasmic Reticulum 1.15 4.37 3.72 2.08 -1.03 -1.14 3.63 -0.43 0.98 -6.68 -1.25 5.53 0.07 1.40 -6.65
Endosome -1.50 1.74 -1.83 -0.49 -2.29 -3.44 -1.26 -5.18 1.13 -3.88 -3.70 -0.58 -5.52 0.93 -5.01
Golgi -1.15 -0.27 -2.09 0.78 -2.07 -2.05 -0.19 -1.70 1.04 -1.71 -2.32 -0.37 -2.92 1.24 -2.76
Lipid Particles -0.94 0.81 -1.19 -0.68 -1.49 -2.21 -0.30 0.06 2.31 0.81 -2.39 -0.23 -0.88 2.21 -1.00
Mitochondria -2.90 -4.52 -7.41 -5.49 -4.47 -3.21 -1.90 -7.69 -2.08 -6.56 -3.66 -3.96 -9.80 -3.08 -7.66
Mitotic Spindle -0.67 -0.29 -2.15 -1.58 -2.04 -3.02 -2.10 -8.89 -5.70 -7.03 -2.92 -1.83 -8.97 -5.96 -7.73
None -2.61 -0.77 -1.33 1.44 -1.00 2.07 -8.66 -3.19 -6.78 7.87 -0.30 -8.70 -4.55 -4.69 7.20
Nuclear Periphery 0.51 -0.43 -5.93 -6.03 -5.89 -1.90 -0.91 -6.44 -5.61 -6.34 -1.99 -1.18 -7.22 -6.36 -7.11
Nuclear Periphery Foci -1.40 1.15 -2.36 0.74 -3.17 -2.42 -1.14 -3.58 -0.86 -2.57 -2.89 -0.87 -4.52 -0.19 -3.88
Nucleolus -2.75 -3.31 9.40 8.83 10.51 -7.60 0.18 7.70 14.11 7.27 -7.22 -1.99 12.86 16.65 13.02
Nucleus 4.53 7.47 4.16 -0.46 -0.92 8.11 10.82 16.15 7.43 4.94 9.50 13.13 16.96 6.76 5.82
Peroxisomes -2.82 -4.26 -1.16 2.56 5.00 -3.35 -1.34 0.23 3.53 1.47 -4.15 -2.21 -0.07 4.21 2.27
Vacuole -0.25 -0.37 -3.70 -3.66 -3.68 -3.44 -2.74 -8.01 -4.92 -5.71 -3.51 -2.76 -8.76 -5.71 -7.06
Vacuole Periphery -1.15 0.26 -2.10 -1.74 -2.11 -4.16 -1.19 -4.38 -0.98 -3.72 -4.35 -1.16 -4.61 -1.91 -4.30
Standard name
Human Ortholog
Description Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Abd1

Abd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Abd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available