ORF
Human Ortholog
Description Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.07 0.06 0.08 0.07 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0
Cytoplasm 0.92 0.9 0.93 0.98 0.9 0.69 0.6 0.68 0.37 0.33 0.21 0.37 0.98 0.96 0.95 0.96 – 0.94 0.73 0.61 0.55 0.57 0.5 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0 0 0 0.05 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.3 0.13 0.62 0.66 0.76 0.43 0 0 0 0 – 0.12 0.12 0.18 0.31 0.23 0.29 0.24
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.09 0.08 0 0 0.08 0.05 0.07 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0.07 0 0.08 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 10 9 1 2 1 4 6 3 0 6 2 0 0 1 0 – 0 3 6 4 0 3 4
Bud 0 8 2 0 1 2 6 4 9 10 19 3 2 0 0 0 – 0 0 4 1 0 2 4
Bud Neck 0 2 9 2 16 14 21 13 12 3 5 5 0 0 0 0 – 0 2 4 2 3 5 7
Bud Site 0 0 0 0 0 0 1 0 2 2 5 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 2 0 17 2 3 3 0 5 2 5 3 4 3 1 2 0 – 1 0 1 2 0 1 2
Cytoplasm 216 260 254 194 196 172 159 132 110 102 66 69 177 179 212 54 – 16 194 220 182 51 104 114
Endoplasmic Reticulum 7 8 4 1 0 11 2 8 7 9 17 19 1 3 1 3 – 0 2 3 4 2 5 5
Endosome 8 2 0 0 3 5 2 1 0 1 0 1 2 2 5 1 – 0 17 10 4 2 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 2 1 0 0 0 2
Mitochondria 1 12 0 3 2 31 79 26 185 203 238 81 2 0 6 2 – 2 31 66 103 21 60 54
Nucleus 0 2 0 2 1 2 3 8 4 3 6 7 0 0 0 1 – 0 0 1 0 0 0 0
Nuclear Periphery 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 5 22 20 5 10 24 15 14 1 0 0 0 – 0 1 3 3 0 0 2
Vac/Vac Membrane 6 4 2 0 7 4 7 8 8 3 3 6 0 3 1 0 – 1 6 27 10 6 18 22
Unique Cell Count 235 289 274 198 218 250 265 195 299 308 312 187 181 186 223 56 17 266 362 330 91 209 229
Labelled Cell Count 243 308 297 206 236 267 304 216 352 365 384 213 189 188 228 61 20 266 362 330 91 209 229


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.6 5.6 5.2 5.4 4.2 4.3 4.5 3.6 3.6 3.4 4.0 4.9 5.0 4.7 6.0 – 6.4 4.5 4.7 4.9
Std Deviation (1e-4) 0.6 0.6 0.6 1.1 1.3 0.8 0.8 0.8 0.5 0.7 0.5 0.8 0.9 1.5 1.1 1.5 – 1.4 0.7 0.9 1.4
Intensity Change (Log2) – – – -0.09 -0.04 -0.43 -0.36 -0.31 -0.64 -0.62 -0.7 -0.47 -0.2 -0.15 -0.24 0.11 – 0.19 -0.32 -0.26 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2948 0.4701 0.4262 -0.1393 0.1787 0.1641 0.9893 1.2598 1.2035 0.5987 1.2196 1.1471 0.4427 0.2474 0.4053 0.6111 -0.0092 -0.1799
Actin 0.0718 0.016 0.0304 0.0074 0.0101 0.0048 0.0401 0.0132 0.0203 0.0022 0.0511 0.008 0.0285 0.0074 0.0127 0.0107 0.003 0.0093
Bud 0.0017 0.0039 0.0017 0.0034 0.0061 0.0009 0.0028 0.007 0.0069 0.0039 0.0032 0.003 0.0022 0.0027 0.0023 0.002 0.0014 0.0008
Bud Neck 0.0089 0.0026 0.007 0.0011 0.004 0.0267 0.0037 0.0016 0.008 0.0011 0.0044 0.0102 0.0121 0.0016 0.0013 0.0058 0.006 0.0126
Bud Periphery 0.0035 0.0072 0.0031 0.0089 0.0188 0.0017 0.0069 0.0113 0.0128 0.0104 0.0043 0.0042 0.0041 0.0059 0.0028 0.0032 0.005 0.0017
Bud Site 0.0196 0.0363 0.0081 0.003 0.0032 0.0009 0.0113 0.0494 0.0256 0.0033 0.0175 0.0025 0.0099 0.0368 0.0264 0.0053 0.0082 0.0009
Cell Periphery 0.0033 0.0027 0.003 0.0015 0.0005 0.0007 0.0015 0.0016 0.0011 0.0012 0.0005 0.0007 0.0016 0.0017 0.0012 0.0014 0.003 0.0004
Cytoplasm 0.1442 0.2837 0.3025 0.3436 0.1046 0.3987 0.1624 0.2369 0.2187 0.1841 0.1338 0.218 0.1973 0.2873 0.2227 0.301 0.3143 0.248
Cytoplasmic Foci 0.1287 0.1331 0.1182 0.1261 0.2205 0.115 0.1688 0.1817 0.1674 0.1506 0.1796 0.1989 0.1084 0.1123 0.1093 0.1455 0.2169 0.1623
Eisosomes 0.0008 0.0008 0.0006 0.0003 0.0002 0.0004 0.0008 0.0003 0.0005 0.0002 0.0003 0.0002 0.0006 0.0005 0.0004 0.0006 0.0002 0.0003
Endoplasmic Reticulum 0.0107 0.0104 0.0094 0.0109 0.0168 0.007 0.0083 0.006 0.0042 0.0047 0.0135 0.0036 0.0169 0.0111 0.0114 0.0065 0.0033 0.0076
Endosome 0.1854 0.1433 0.1478 0.2212 0.1897 0.1179 0.2042 0.1625 0.1591 0.2344 0.1831 0.1709 0.176 0.1398 0.1839 0.212 0.2426 0.1738
Golgi 0.0299 0.0296 0.0525 0.0328 0.0613 0.0293 0.0621 0.0505 0.0432 0.0587 0.0724 0.0625 0.0286 0.0315 0.0365 0.0658 0.0133 0.0462
Lipid Particles 0.0243 0.0115 0.0236 0.0102 0.0056 0.0152 0.019 0.0072 0.0091 0.0346 0.0115 0.0089 0.0233 0.0092 0.0096 0.011 0.0011 0.0216
Mitochondria 0.0378 0.023 0.0489 0.094 0.081 0.04 0.0999 0.084 0.1099 0.1513 0.134 0.1127 0.0533 0.0374 0.0806 0.1136 0.0037 0.0551
None 0.2561 0.2606 0.1989 0.1029 0.2288 0.2164 0.1491 0.1369 0.1384 0.0983 0.114 0.1335 0.2238 0.2742 0.2167 0.0737 0.1493 0.224
Nuclear Periphery 0.0099 0.0031 0.0029 0.0036 0.0048 0.0008 0.0046 0.0024 0.005 0.0013 0.0116 0.0009 0.02 0.0016 0.0059 0.0017 0.0005 0.0012
Nucleolus 0.0029 0.001 0.0007 0.0008 0.0004 0.0003 0.0007 0.0015 0.0013 0.0005 0.0009 0.0005 0.016 0.0018 0.0035 0.0008 0.0001 0.0003
Nucleus 0.0191 0.0023 0.0054 0.003 0.0015 0.0012 0.0067 0.0028 0.009 0.0021 0.0032 0.0026 0.0111 0.0026 0.0274 0.0017 0.0007 0.0009
Peroxisomes 0.0133 0.0112 0.0201 0.0093 0.0186 0.0125 0.0278 0.0227 0.0377 0.0399 0.043 0.0455 0.0209 0.0156 0.0196 0.011 0.0195 0.0234
Punctate Nuclear 0.0037 0.002 0.0028 0.0022 0.0012 0.0013 0.0027 0.0018 0.0058 0.0013 0.0037 0.0036 0.0155 0.0036 0.0089 0.0007 0.0005 0.0017
Vacuole 0.0192 0.0129 0.01 0.0105 0.0155 0.0066 0.0127 0.0143 0.0123 0.0118 0.009 0.0065 0.023 0.0123 0.0131 0.0212 0.0052 0.0054
Vacuole Periphery 0.0054 0.0027 0.0024 0.0031 0.0071 0.002 0.0039 0.0043 0.0037 0.0042 0.0052 0.0026 0.0071 0.0031 0.0038 0.0048 0.002 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.1083 25.6027 12.3322 23.2028 22.8012 18.0786 19.8693 17.1653 12.8002 24.6297
Translational Efficiency 0.7273 0.5452 0.9248 0.2951 0.5616 0.7134 0.4993 0.4433 0.7351 0.6902

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1056 1120 216 777 1589 2125 114 1268 2645 3245 330 2045

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 629.18 809.75 946.00 815.50 736.60 777.98 918.29 818.69 693.71 788.95 936.43 817.48
Standard Deviation 73.65 110.36 109.32 103.71 87.02 99.47 89.88 125.91 97.38 104.46 103.86 117.98
Intensity Change Log 2 — 0.364004 0.588367 0.374212 — 0.078852 0.318068 0.152436 — 0.217240 0.448901 0.258851

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000157 0.000562 0.001312 0.005064 0.000243 0.000608 0.000410 0.003029 0.000209 0.000592 0.001001 0.003802
Bud Neck 0.019305 0.027195 0.006864 0.019385 0.021971 0.043208 0.007460 0.015174 0.020907 0.037681 0.007070 0.016774
Bud Site 0.007326 0.023986 0.032685 0.076420 0.006998 0.030655 0.003811 0.051905 0.007129 0.028353 0.022711 0.061219
Cell Periphery 0.000184 0.000184 0.000389 0.000205 0.000098 0.000118 0.000305 0.000245 0.000132 0.000141 0.000360 0.000230
Cytoplasm 0.502401* 0.401681* 0.183306* 0.360723* 0.480752* 0.373277* 0.225617* 0.449358* 0.489395* 0.383081* 0.197922* 0.415681*
Cytoplasmic Foci 0.258716* 0.248187* 0.017272 0.090382 0.224933* 0.230794* 0.039267 0.056203 0.238421* 0.236797* 0.024870 0.069190
Eisosomes 0.000267 0.000142 0.000149 0.000110 0.000136 0.000115 0.000166 0.000078 0.000188 0.000125 0.000155 0.000090
Endoplasmic Reticulum 0.001601 0.001437 0.015369 0.004084 0.001336 0.000819 0.005517 0.002606 0.001441 0.001032 0.011966 0.003168
Endosome 0.020885 0.044794 0.008413 0.015487 0.018335 0.046541 0.009407 0.012057 0.019353 0.045938 0.008756 0.013360
Golgi 0.003396 0.007342 0.000250 0.004516 0.003175 0.009674 0.000143 0.005188 0.003263 0.008869 0.000213 0.004933
Lipid Particles 0.009276 0.006103 0.006406 0.003207 0.006865 0.005913 0.007339 0.002103 0.007828 0.005979 0.006728 0.002522
Mitochondria 0.004229 0.006995 0.000831 0.005702 0.003872 0.016105 0.000832 0.003995 0.004015 0.012961 0.000831 0.004644
Mitotic Spindle 0.000101 0.008344 0.003263 0.055674 0.000459 0.011227 0.000356 0.035505 0.000316 0.010232 0.002259 0.043169
None 0.003217 0.002931 0.006304 0.002986 0.001109 0.001633 0.002769 0.003956 0.001950 0.002081 0.005083 0.003588
Nuclear Periphery 0.000286 0.000552 0.003099 0.001649 0.000407 0.000574 0.002291 0.001491 0.000359 0.000566 0.002820 0.001551
Nuclear Periphery Foci 0.000428 0.001165 0.004528 0.002603 0.000281 0.000224 0.004468 0.001417 0.000340 0.000549 0.004508 0.001868
Nucleolus 0.001128 0.001196 0.001756 0.001253 0.000717 0.001272 0.001452 0.000859 0.000881 0.001246 0.001651 0.001009
Nucleus 0.087396 0.069682 0.543977* 0.124288 0.121909 0.059120 0.486624* 0.156789* 0.108130 0.062766 0.524164* 0.144441*
Peroxisomes 0.003968 0.015133 0.000951 0.012976 0.004813 0.013744 0.000747 0.006909 0.004476 0.014224 0.000881 0.009214
Vacuole 0.073780 0.129819 0.162280 0.210476* 0.100148 0.149461 0.200606* 0.187495 0.089621 0.142682 0.175520 0.196226
Vacuole Periphery 0.001953 0.002568 0.000597 0.002808 0.001444 0.004917 0.000413 0.003637 0.001647 0.004106 0.000534 0.003322

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.57 -9.26 -3.43 -3.03 -1.92 -2.27 -3.00 -3.76 -2.92 -2.91 -3.63 -9.01 -5.03 -4.18 -2.73
Bud Neck -2.85 6.41 0.60 3.40 -5.83 -8.50 4.37 4.94 13.79 -2.02 -8.93 8.16 4.29 13.31 -5.09
Bud Site -8.17 -1.93 -9.22 -5.51 -3.74 -11.24 1.88 -9.13 -1.34 -9.63 -13.74 -1.73 -12.94 -4.52 -5.13
Cell Periphery 0.14 -7.62 1.03 0.40 7.83 -2.24 -7.54 -4.73 -3.51 3.79 -0.61 -11.59 -3.70 -2.62 7.99
Cytoplasm 8.92 25.43 16.27 7.39 -10.77 11.85 14.88 9.32 -1.13 -9.67 15.05 29.29 17.11 3.57 -16.56
Cytoplasmic Foci 1.69 39.61 27.19 24.80 -12.60 -0.67 29.73 33.67 36.94 -2.19 0.74 51.48 43.29 43.96 -11.55
Eisosomes 5.47 3.63 6.99 2.70 5.54 2.64 -3.59 8.57 5.87 8.02 6.19 0.35 9.98 5.86 10.45
Endoplasmic Reticulum 0.37 -12.55 -6.42 -8.55 10.40 3.37 -6.57 -8.48 -12.49 4.55 2.20 -13.65 -10.10 -14.77 11.32
Endosome -7.72 7.18 5.95 12.36 -0.88 -12.59 5.21 7.21 18.98 0.85 -14.67 9.15 9.39 22.58 0.19
Golgi -4.51 9.38 1.66 4.94 -2.92 -7.85 7.65 0.68 6.43 -2.91 -9.09 11.19 1.36 8.08 -3.86
Lipid Particles 3.65 3.24 7.86 4.30 4.86 1.61 -0.77 11.03 7.16 6.29 3.66 1.74 13.17 8.22 8.52
Mitochondria -1.53 4.06 0.53 1.80 -2.33 -9.74 7.67 2.39 10.47 -3.52 -8.73 8.12 1.73 9.31 -4.02
Mitotic Spindle -4.52 -2.55 -8.73 -6.76 -8.11 -7.80 0.06 -8.85 -4.96 -8.84 -9.01 -2.36 -12.40 -8.23 -11.56
None 0.33 -0.23 0.47 0.04 0.45 -1.27 -1.62 -2.84 -1.81 -0.41 -0.23 -1.12 -1.90 -1.48 0.26
Nuclear Periphery -3.03 -18.47 -11.70 -9.14 5.41 -1.19 -11.20 -14.32 -9.33 1.35 -2.21 -20.98 -18.52 -12.59 5.80
Nuclear Periphery Foci -2.06 -9.93 -6.76 -2.96 5.18 2.02 -4.82 -10.59 -11.11 3.49 -1.67 -10.52 -11.40 -7.66 6.95
Nucleolus -0.19 -0.71 -0.37 -0.35 0.43 -4.41 -2.01 -1.07 2.79 1.36 -2.04 -2.10 -0.69 1.61 1.71
Nucleus 3.38 -21.66 -8.84 -10.83 17.59 15.08 -11.79 -10.65 -21.05 9.02 14.21 -23.78 -13.85 -23.42 18.91
Peroxisomes -8.76 8.02 -3.79 3.80 -6.78 -9.31 10.08 0.78 8.41 -4.76 -12.75 12.68 -1.97 8.77 -8.07
Vacuole -11.08 -9.83 -22.82 -15.67 -4.14 -9.85 -6.78 -20.35 -13.57 0.08 -14.67 -11.38 -30.16 -20.30 -2.53
Vacuole Periphery -1.57 5.61 1.05 2.14 -2.03 -10.63 9.90 -0.94 4.74 -2.97 -9.71 9.72 -0.26 5.35 -3.47
ORF
Human Ortholog
Description Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (46%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YBR238C

YBR238C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr238c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available