Standard name | |
---|---|
Human Ortholog | |
Description | ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 2 | 5 | 2 | 5 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 5 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 5 | 11 | 8 | 19 | 13 | 16 | 10 | 15 | 0 | 3 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 14 | 5 | 10 | 14 | 3 | 10 | 12 | 10 | 7 | 11 | 2 | 31 | 59 | 55 | 29 | 8 | 22 | 2 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 5 | 2 | 1 | 0 | 3 | 2 | 1 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 0 | 14 | 20 | 11 | 8 | 46 | 55 | 92 | 98 | 104 | 59 | 64 | 2 | 0 | 4 | 6 | 0 | 7 | 0 | 3 | 1 | 1 | 3 | 5 |
Nucleus | 50 | 362 | 425 | 196 | 269 | 317 | 320 | 401 | 211 | 233 | 195 | 244 | 312 | 368 | 368 | 224 | 127 | 240 | 47 | 388 | 428 | 177 | 144 | 209 |
Nuclear Periphery | 0 | 0 | 4 | 0 | 2 | 3 | 5 | 4 | 9 | 8 | 0 | 4 | 1 | 2 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 23 | 104 | 99 | 71 | 71 | 147 | 168 | 269 | 187 | 208 | 157 | 236 | 84 | 114 | 134 | 56 | 20 | 67 | 13 | 33 | 54 | 60 | 111 | 153 |
Peroxisomes | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 3 | 2 | 3 | 0 | 2 | 4 | 9 | 11 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 4 |
Vac/Vac Membrane | 1 | 10 | 4 | 2 | 5 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 6 | 3 | 7 | 7 | 10 | 1 | 12 | 10 | 12 | 18 | 37 |
Unique Cell Count | 64 | 423 | 482 | 238 | 324 | 421 | 439 | 615 | 375 | 409 | 314 | 396 | 370 | 475 | 493 | 296 | 158 | 315 | 68 | 448 | 505 | 258 | 287 | 426 |
Labelled Cell Count | 78 | 509 | 562 | 291 | 380 | 531 | 572 | 811 | 533 | 584 | 437 | 575 | 438 | 568 | 596 | 339 | 167 | 353 | 68 | 448 | 505 | 258 | 287 | 426 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 10.8 | 7.9 | 7.5 | 8.0 | 6.9 | 6.8 | 6.3 | 6.3 | 6.1 | 6.1 | 5.7 | 11.7 | 11.3 | 9.8 | 15.5 | 18.1 | 15.6 | 11.4 | 12.4 | 12.9 |
Std Deviation (1e-4) | 3.1 | 3.9 | 2.1 | 2.0 | 2.2 | 1.9 | 2.1 | 1.6 | 1.8 | 2.0 | 2.2 | 1.7 | 3.8 | 4.2 | 3.5 | 5.7 | 5.9 | 4.8 | 2.9 | 2.8 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.01 | -0.21 | -0.23 | -0.33 | -0.33 | -0.38 | -0.39 | -0.49 | 0.57 | 0.51 | 0.31 | 0.96 | 1.19 | 0.98 | 0.52 | 0.65 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.6 | 0 | 3.9 | 4.1 | 4.4 | 0 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 3.0 | 0 | 1.5 | 1.2 | 1.8 | 0.9 | 2.4 | 0 | 5.3 | 7.0 | 6.6 | 5.8 | 0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.3 | -1.3 | 3.9 | 4.6 | 5.9 | 9.3 | 9.1 | 6.8 | 6.0 | -3.3 | -4.5 | -3.4 | -1.6 | 0 | -1.5 |
Nucleus | -2.1 | -2.1 | -5.0 | -5.9 | -8.8 | -10.6 | -10.6 | -8.7 | -9.2 | -1.6 | -4.4 | -5.4 | -4.6 | -2.5 | -4.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1.9 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.8 | 0.5 | 4.8 | 5.9 | 8.1 | 9.0 | 9.5 | 8.7 | 11.9 | 0.8 | 1.3 | 2.4 | -0.5 | -2.2 | 0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.6123 | 9.4563 | 8.8985 | 7.9385 | 8.3627 | 9.2581 | 12.4653 | 11.936 | 12.1342 | 11.0683 | 10.5306 | 11.2351 | 14.084 | 12.0718 | 12.2997 | 10.7105 | 9.5618 | 11.5154 |
Actin | 0.0265 | 0 | 0.0264 | 0.0373 | 0.068 | 0.0005 | 0.0247 | 0.0049 | 0.012 | 0.0035 | 0.0098 | 0.0038 | 0 | 0 | 0 | 0.0001 | 0.001 | 0.0002 |
Bud | 0.001 | 0 | 0.0005 | 0.0007 | 0.0017 | 0.0001 | 0.0008 | 0 | 0.0005 | 0.0006 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0032 | 0.0001 | 0 |
Bud Neck | 0.0066 | 0 | 0.0004 | 0.0017 | 0.0012 | 0.0004 | 0.0019 | 0.0001 | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0.0007 | 0.0004 | 0.0003 |
Bud Periphery | 0.0019 | 0 | 0.0009 | 0.0017 | 0.0023 | 0.0001 | 0.0017 | 0.0001 | 0.001 | 0.001 | 0.0007 | 0.0004 | 0 | 0 | 0 | 0.0083 | 0.0002 | 0.0001 |
Bud Site | 0.0089 | 0 | 0.0037 | 0.0378 | 0.0136 | 0.0001 | 0.0069 | 0.0001 | 0.0031 | 0.0011 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0.0012 | 0.0002 | 0.0001 |
Cell Periphery | 0.0013 | 0 | 0.0008 | 0.0015 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0028 | 0.0001 | 0 |
Cytoplasm | 0.0021 | 0 | 0.0004 | 0.0011 | 0.0032 | 0 | 0.0052 | 0.0022 | 0.0047 | 0.0002 | 0.0074 | 0.0009 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0 |
Cytoplasmic Foci | 0.002 | 0 | 0.0044 | 0.0087 | 0.0182 | 0.0002 | 0.0202 | 0.0002 | 0.0052 | 0.0018 | 0.0142 | 0.0011 | 0 | 0 | 0 | 0.0001 | 0.0039 | 0.0002 |
Eisosomes | 0.0008 | 0 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0107 | 0 | 0.0006 | 0.0011 | 0.0024 | 0.0001 | 0.0031 | 0.0001 | 0.0018 | 0.0002 | 0.0042 | 0.0007 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endosome | 0.0029 | 0 | 0.0012 | 0.0151 | 0.0091 | 0.0009 | 0.0196 | 0.0002 | 0.0032 | 0.0085 | 0.0281 | 0.0038 | 0 | 0 | 0 | 0.0001 | 0.005 | 0.0001 |
Golgi | 0.0036 | 0 | 0.0018 | 0.0058 | 0.0039 | 0.0004 | 0.0061 | 0.0005 | 0.0018 | 0.0057 | 0.0117 | 0.0014 | 0 | 0 | 0 | 0 | 0.0072 | 0.0001 |
Lipid Particles | 0.0053 | 0 | 0.007 | 0.0117 | 0.0121 | 0.0016 | 0.0127 | 0.0001 | 0.0052 | 0.0015 | 0.0121 | 0.0019 | 0 | 0 | 0 | 0 | 0.0569 | 0.0064 |
Mitochondria | 0.0083 | 0.0001 | 0.0158 | 0.0191 | 0.0025 | 0.0003 | 0.0128 | 0.0005 | 0.004 | 0.0086 | 0.0026 | 0.0078 | 0.0002 | 0.0001 | 0.0001 | 0.0056 | 0.0284 | 0.0088 |
None | 0.0015 | 0 | 0.0006 | 0.0021 | 0.0072 | 0.0001 | 0.003 | 0.0027 | 0.0022 | 0.0001 | 0.0058 | 0.0005 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0117 | 0.0001 | 0.0016 | 0.0106 | 0.006 | 0.0023 | 0.0107 | 0.0005 | 0.0035 | 0.0003 | 0.0286 | 0.0041 | 0 | 0.0004 | 0.0001 | 0.0007 | 0.0008 | 0.0004 |
Nucleolus | 0.1156 | 0.0445 | 0.0419 | 0.0565 | 0.3309 | 0.1975 | 0.0387 | 0.0258 | 0.0243 | 0.0216 | 0.222 | 0.0976 | 0.0212 | 0.0542 | 0.0155 | 0.0832 | 0.1421 | 0.1269 |
Nucleus | 0.7793 | 0.955 | 0.8807 | 0.7544 | 0.4925 | 0.7933 | 0.7975 | 0.959 | 0.9156 | 0.9428 | 0.6157 | 0.8671 | 0.9786 | 0.9451 | 0.9843 | 0.8824 | 0.7436 | 0.8549 |
Peroxisomes | 0.0005 | 0 | 0.0069 | 0.0064 | 0.0027 | 0.0002 | 0.0059 | 0.0001 | 0.0012 | 0.0009 | 0.0063 | 0.0006 | 0 | 0 | 0 | 0.0002 | 0.0076 | 0.0003 |
Punctate Nuclear | 0.0029 | 0.0001 | 0.0009 | 0.0116 | 0.019 | 0.0015 | 0.0214 | 0.0017 | 0.0073 | 0.0002 | 0.0235 | 0.0066 | 0 | 0.0001 | 0 | 0.0002 | 0.0007 | 0.0004 |
Vacuole | 0.0029 | 0 | 0.0016 | 0.0035 | 0.0016 | 0.0002 | 0.0039 | 0.0004 | 0.0014 | 0.0007 | 0.0029 | 0.0004 | 0 | 0 | 0 | 0.0069 | 0.0007 | 0.0001 |
Vacuole Periphery | 0.004 | 0 | 0.0016 | 0.0111 | 0.001 | 0.0001 | 0.0024 | 0.0005 | 0.001 | 0.0005 | 0.0025 | 0.0005 | 0 | 0 | 0 | 0.0035 | 0.0009 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.6289 | 37.2437 | 39.5115 | 39.6963 | 54.2282 | 61.1579 | 34.8622 | 33.6563 | 34.7739 | 50.3044 |
Translational Efficiency | 1.2697 | 1.4586 | 0.9832 | 1.1747 | 1.1154 | 1.3127 | 1.1316 | 0.851 | 1.0005 | 1.0633 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
317 | 1287 | 364 | 484 | 1034 | 1446 | 301 | 93 | 1351 | 2733 | 665 | 577 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 859.63 | 1135.80 | 1316.48 | 1355.35 | 984.91 | 1115.01 | 1443.12 | 1714.90 | 955.51 | 1124.80 | 1373.80 | 1413.30 |
Standard Deviation | 160.44 | 245.63 | 200.07 | 280.60 | 187.16 | 223.82 | 223.76 | 352.86 | 188.86 | 234.58 | 220.33 | 321.86 |
Intensity Change Log 2 | — | 0.401921 | 0.614898 | 0.656878 | — | 0.178993 | 0.551127 | 0.800061 | — | 0.287183 | 0.581198 | 0.735095 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000497 | 0.000543 | 0.000869 | 0.000900 | 0.000562 | 0.000562 | 0.000921 | 0.000902 | 0.000547 | 0.000553 | 0.000893 | 0.000900 |
Bud Neck | 0.003581 | 0.005348 | 0.010614 | 0.014384 | 0.003709 | 0.007188 | 0.010768 | 0.011484 | 0.003679 | 0.006322 | 0.010684 | 0.013917 |
Bud Site | 0.001579 | 0.001206 | 0.001063 | 0.001811 | 0.000530 | 0.001198 | 0.001028 | 0.001151 | 0.000776 | 0.001202 | 0.001047 | 0.001705 |
Cell Periphery | 0.000059 | 0.000043 | 0.000026 | 0.000075 | 0.000035 | 0.000065 | 0.000025 | 0.000035 | 0.000041 | 0.000055 | 0.000026 | 0.000069 |
Cytoplasm | 0.000400 | 0.000501 | 0.000088 | 0.000373 | 0.000088 | 0.000150 | 0.000080 | 0.000168 | 0.000161 | 0.000315 | 0.000084 | 0.000340 |
Cytoplasmic Foci | 0.000830 | 0.000268 | 0.000055 | 0.000368 | 0.000349 | 0.000601 | 0.000079 | 0.000122 | 0.000462 | 0.000444 | 0.000066 | 0.000328 |
Eisosomes | 0.000058 | 0.000089 | 0.000077 | 0.000093 | 0.000090 | 0.000116 | 0.000117 | 0.000075 | 0.000083 | 0.000103 | 0.000095 | 0.000090 |
Endoplasmic Reticulum | 0.001274 | 0.000689 | 0.001051 | 0.000603 | 0.000974 | 0.001086 | 0.000585 | 0.000344 | 0.001044 | 0.000899 | 0.000840 | 0.000561 |
Endosome | 0.000277 | 0.000061 | 0.000032 | 0.000452 | 0.000047 | 0.000477 | 0.000019 | 0.000021 | 0.000101 | 0.000281 | 0.000027 | 0.000382 |
Golgi | 0.000182 | 0.000267 | 0.000113 | 0.000472 | 0.000246 | 0.000309 | 0.000099 | 0.000079 | 0.000231 | 0.000290 | 0.000106 | 0.000408 |
Lipid Particles | 0.000217 | 0.000104 | 0.000023 | 0.000031 | 0.000084 | 0.000623 | 0.000014 | 0.000013 | 0.000115 | 0.000379 | 0.000019 | 0.000028 |
Mitochondria | 0.000756 | 0.002019 | 0.001194 | 0.002313 | 0.001123 | 0.001216 | 0.001632 | 0.003188 | 0.001037 | 0.001594 | 0.001392 | 0.002454 |
Mitotic Spindle | 0.002169 | 0.001861 | 0.000211 | 0.006037 | 0.000179 | 0.004451 | 0.001337 | 0.000120 | 0.000646 | 0.003231 | 0.000721 | 0.005083 |
None | 0.001114 | 0.001244 | 0.000861 | 0.001880 | 0.000723 | 0.001047 | 0.000858 | 0.000879 | 0.000815 | 0.001140 | 0.000860 | 0.001718 |
Nuclear Periphery | 0.000041 | 0.000048 | 0.000045 | 0.000407 | 0.000030 | 0.000102 | 0.000058 | 0.000121 | 0.000033 | 0.000076 | 0.000051 | 0.000361 |
Nuclear Periphery Foci | 0.000355 | 0.000178 | 0.000041 | 0.000282 | 0.000099 | 0.000724 | 0.000012 | 0.000016 | 0.000159 | 0.000467 | 0.000028 | 0.000239 |
Nucleolus | 0.094260 | 0.089548 | 0.090498 | 0.089516 | 0.081504 | 0.090765 | 0.089907 | 0.084621 | 0.084497 | 0.090192 | 0.090230 | 0.088727 |
Nucleus | 0.888581* | 0.893313* | 0.888350* | 0.873679* | 0.907371* | 0.886658* | 0.886132* | 0.891000* | 0.902963* | 0.889792* | 0.887346* | 0.876470* |
Peroxisomes | 0.003198 | 0.002108 | 0.004329 | 0.005158 | 0.001687 | 0.001812 | 0.005886 | 0.005065 | 0.002042 | 0.001952 | 0.005034 | 0.005143 |
Vacuole | 0.000350 | 0.000252 | 0.000302 | 0.000737 | 0.000267 | 0.000466 | 0.000279 | 0.000305 | 0.000287 | 0.000365 | 0.000291 | 0.000667 |
Vacuole Periphery | 0.000223 | 0.000312 | 0.000157 | 0.000432 | 0.000302 | 0.000383 | 0.000164 | 0.000291 | 0.000283 | 0.000350 | 0.000160 | 0.000409 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.50 | -4.38 | -5.31 | -7.61 | -0.73 | 0.20 | -2.51 | -2.04 | -2.76 | -0.48 | 0.14 | -3.06 | -3.25 | -8.41 | -0.58 |
Bud Neck | -1.80 | -6.44 | -7.53 | -7.15 | -2.95 | -5.46 | -9.43 | -3.80 | -2.64 | -1.01 | -5.12 | -11.50 | -9.12 | -7.27 | -3.17 |
Bud Site | 0.21 | 0.24 | -0.51 | -1.26 | -2.35 | -1.75 | -3.22 | -2.17 | -1.12 | -0.79 | -0.93 | -1.64 | -2.92 | -1.96 | -2.32 |
Cell Periphery | 0.42 | 1.15 | -0.51 | -1.79 | -3.98 | -1.58 | 0.69 | -0.98 | 0.50 | -1.58 | -1.11 | 1.29 | -2.15 | -1.09 | -4.25 |
Cytoplasm | -0.26 | 1.52 | 0.34 | 0.67 | -3.27 | -0.26 | 0.38 | -0.86 | -0.76 | -1.64 | -0.94 | 1.29 | -1.38 | -0.10 | -3.51 |
Cytoplasmic Foci | 1.02 | 1.28 | 0.93 | -0.52 | -2.60 | -0.06 | 0.95 | 0.63 | 1.22 | -1.33 | 0.57 | 1.47 | 0.83 | 0.61 | -2.70 |
Eisosomes | -3.62 | -2.19 | -3.85 | -0.83 | -2.47 | -3.25 | -3.79 | 0.49 | 2.81 | 3.28 | -3.33 | -2.36 | -1.37 | 1.40 | 0.57 |
Endoplasmic Reticulum | 3.79 | 0.61 | 3.60 | -0.06 | 3.71 | -1.08 | 3.18 | 2.01 | 2.44 | 0.76 | 1.89 | 0.84 | 4.39 | 2.80 | 3.20 |
Endosome | 1.37 | 1.52 | -0.08 | -1.07 | -1.17 | -2.48 | 1.18 | 0.68 | 2.61 | -0.63 | -1.60 | 1.76 | -0.80 | -0.11 | -1.20 |
Golgi | -0.35 | 1.16 | -0.66 | -0.47 | -1.12 | -0.23 | 0.75 | 0.78 | 1.32 | 0.32 | -0.30 | 0.90 | -0.48 | -0.31 | -1.13 |
Lipid Particles | 0.89 | 1.30 | 1.29 | 1.24 | -0.30 | -1.35 | 1.39 | 1.21 | 1.56 | -0.74 | -1.08 | 1.83 | 1.69 | 1.71 | -0.93 |
Mitochondria | -1.84 | -3.11 | -8.54 | -0.69 | -7.15 | -0.15 | -1.54 | -1.99 | -1.98 | -1.59 | -1.16 | -1.33 | -4.16 | -2.64 | -4.81 |
Mitotic Spindle | 0.24 | 1.09 | -1.45 | -1.92 | -2.67 | -2.66 | -0.84 | 0.68 | 3.03 | 0.99 | -2.28 | -0.38 | -2.28 | -1.09 | -1.83 |
None | -0.42 | 0.77 | -2.29 | -1.71 | -4.28 | -0.93 | -1.56 | -1.38 | 0.19 | -0.28 | -1.41 | -0.87 | -4.44 | -2.14 | -4.32 |
Nuclear Periphery | -0.45 | -1.20 | -2.04 | -2.01 | -1.95 | -3.06 | -2.43 | -2.23 | -1.14 | -1.45 | -3.08 | -3.00 | -2.23 | -1.95 | -2.01 |
Nuclear Periphery Foci | 0.95 | 1.38 | 0.86 | -0.11 | -1.08 | -1.52 | 1.60 | 1.49 | 1.75 | -0.27 | -1.14 | 1.74 | 0.07 | 1.12 | -1.22 |
Nucleolus | 0.76 | -0.26 | -0.25 | -1.02 | -0.00 | -1.95 | -1.92 | -0.97 | -0.39 | 0.01 | -1.40 | -2.21 | -1.79 | -1.07 | -0.02 |
Nucleus | -0.76 | 0.59 | 2.03 | 3.32 | 1.66 | 3.57 | 3.51 | 1.96 | 0.83 | 0.16 | 2.63 | 3.85 | 4.53 | 3.22 | 1.54 |
Peroxisomes | 1.43 | -0.35 | -1.14 | -5.21 | -1.40 | -0.05 | -6.47 | -3.76 | -3.86 | 0.60 | 0.90 | -5.48 | -4.98 | -6.46 | -0.41 |
Vacuole | 0.44 | -0.08 | -1.24 | -1.75 | -1.39 | -0.92 | -0.72 | -1.29 | -0.24 | -0.99 | -0.56 | -0.76 | -1.79 | -1.49 | -1.56 |
Vacuole Periphery | -0.86 | 0.66 | -1.31 | -0.90 | -2.23 | -0.65 | 1.56 | -0.44 | 0.30 | -3.49 | -0.72 | 1.74 | -0.99 | -0.59 | -2.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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