Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 4 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 6 | 3 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 7 | 9 | 5 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 6 | 7 | 8 | 8 | 7 | 7 | 11 | 11 | 4 | 4 | 3 | 14 | 14 | 16 | 20 | 24 | 13 | 0 | 1 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 12 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 3 | 1 | 11 | 3 | 16 | 10 | 12 | 11 | 10 | 5 | 5 | 7 | 6 | 6 | 9 | 39 | 23 | 15 | 3 | 9 | 2 | 5 | 15 | 18 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 2 | 1 | 1 | 5 | 5 |
Mitochondria | 24 | 49 | 55 | 21 | 45 | 115 | 125 | 224 | 224 | 167 | 134 | 192 | 0 | 0 | 3 | 37 | 29 | 18 | 6 | 12 | 11 | 14 | 47 | 56 |
Nucleus | 10 | 19 | 5 | 3 | 6 | 3 | 7 | 19 | 19 | 15 | 12 | 12 | 8 | 8 | 7 | 19 | 27 | 10 | 3 | 5 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 221 | 244 | 180 | 77 | 144 | 155 | 204 | 201 | 215 | 199 | 143 | 205 | 112 | 109 | 125 | 157 | 145 | 111 | 289 | 278 | 252 | 100 | 238 | 152 |
Nucleolus | 36 | 27 | 53 | 24 | 42 | 87 | 94 | 144 | 144 | 121 | 101 | 118 | 13 | 13 | 13 | 54 | 42 | 36 | 4 | 15 | 2 | 2 | 3 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 5 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 5 | 9 |
SpindlePole | 1 | 0 | 2 | 0 | 3 | 3 | 7 | 8 | 8 | 4 | 1 | 2 | 2 | 2 | 5 | 12 | 13 | 7 | 1 | 2 | 3 | 3 | 4 | 3 |
Vac/Vac Membrane | 103 | 87 | 51 | 6 | 38 | 20 | 24 | 24 | 27 | 16 | 8 | 12 | 33 | 35 | 45 | 81 | 77 | 61 | 10 | 21 | 4 | 4 | 10 | 9 |
Unique Cell Count | 298 | 323 | 264 | 104 | 225 | 285 | 346 | 477 | 482 | 342 | 270 | 360 | 147 | 143 | 173 | 294 | 284 | 199 | 328 | 360 | 292 | 142 | 341 | 269 |
Labelled Cell Count | 405 | 433 | 368 | 143 | 304 | 404 | 489 | 657 | 674 | 540 | 416 | 555 | 189 | 188 | 227 | 424 | 398 | 279 | 328 | 360 | 292 | 142 | 341 | 269 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 11.5 | 11.3 | 10.0 | 9.2 | 7.9 | 7.6 | 7.7 | 7.7 | 6.8 | 7.1 | 7.0 | 10.8 | 10.8 | 10.9 | 7.1 | 7.3 | 9.7 | 11.7 | 12.4 | 12.8 |
Std Deviation (1e-4) | 2.7 | 2.9 | 3.3 | 2.6 | 2.2 | 2.5 | 2.2 | 2.6 | 2.6 | 1.9 | 2.0 | 2.0 | 3.0 | 3.0 | 3.5 | 1.3 | 1.9 | 2.4 | 2.6 | 2.5 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.3 | -0.52 | -0.57 | -0.55 | -0.55 | -0.72 | -0.67 | -0.68 | -0.07 | -0.07 | -0.06 | -0.68 | -0.63 | -0.22 | 0.04 | 0.14 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.6 | -0.1 | -0.5 | -0.3 | -0.3 | 0 | -1.0 | 0 | 3.0 | 3.1 | 3.0 | 2.3 | 2.9 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 |
Endosome | 0 | 1.4 | -0.4 | -0.4 | -1.4 | -1.7 | -2.1 | -1.6 | -1.6 | 0 | 0 | 0.5 | 3.8 | 1.9 | 1.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.1 | -0.2 | 4.9 | 4.1 | 7.0 | 6.9 | 7.1 | 7.0 | 8.2 | -5.9 | -5.9 | -5.8 | -2.6 | -3.4 | -3.4 |
Nucleus | 0 | 0.6 | 0 | 0.1 | 1.5 | 1.5 | 1.7 | 1.7 | 1.1 | 0 | 0 | 0 | 2.7 | 3.8 | 1.9 |
Nuclear Periphery | 1.1 | -1.0 | -3.3 | -2.3 | -6.8 | -6.2 | -2.5 | -3.6 | -2.9 | 1.7 | 1.7 | 0.9 | -3.6 | -4.1 | -2.7 |
Nucleolus | 0.6 | -0.4 | 2.8 | 2.0 | 3.0 | 2.9 | 4.1 | 4.4 | 3.5 | -3.0 | -2.9 | -3.6 | -0.5 | -1.6 | -0.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.7 | 0 |
Vacuole | -3.2 | -0.7 | -4.3 | -4.6 | -6.2 | -5.9 | -5.7 | -6.0 | -6.5 | 0.8 | 1.2 | 1.7 | 2.3 | 2.2 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.4948 | 7.1391 | 6.2739 | 6.3773 | 5.6201 | 6.1661 | 8.2773 | 8.6007 | 7.5013 | 6.3457 | 6.6794 | 7.4757 | 10.2296 | 8.9191 | 8.9538 | 8.8107 | 9.407 | 8.6231 |
Actin | 0.0026 | 0.0003 | 0.0007 | 0.0015 | 0.0013 | 0.0001 | 0.0036 | 0.0004 | 0.0008 | 0.001 | 0.0073 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0001 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Site | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0013 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0011 | 0 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0038 | 0.0011 | 0.0005 | 0.0013 | 0.0002 | 0.0001 | 0.0014 | 0.0033 | 0.0002 | 0.0009 | 0.0025 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0019 | 0.0014 | 0.001 | 0.0019 | 0.0016 | 0.0003 | 0.0021 | 0.001 | 0.0016 | 0.0013 | 0.0007 | 0.0003 | 0.0003 | 0.0006 | 0.0007 | 0.0009 | 0.0004 | 0.0002 |
Endosome | 0.0055 | 0.0006 | 0.0009 | 0.0032 | 0.0022 | 0.0009 | 0.0037 | 0.0015 | 0.0014 | 0.0005 | 0.0014 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0002 |
Golgi | 0.0013 | 0.001 | 0.0004 | 0.0009 | 0.0013 | 0.0003 | 0.0016 | 0.0057 | 0.0007 | 0.0005 | 0.0043 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0002 |
Lipid Particles | 0.0194 | 0.0453 | 0.0093 | 0.0058 | 0.0177 | 0.0067 | 0.0032 | 0.0262 | 0.0085 | 0.0042 | 0.0122 | 0.0037 | 0.0024 | 0.0032 | 0.0032 | 0.0021 | 0.0047 | 0.002 |
Mitochondria | 0.0093 | 0.0018 | 0.0019 | 0.0021 | 0.0071 | 0.0011 | 0.0147 | 0.006 | 0.0025 | 0.0035 | 0.0083 | 0.0033 | 0.0011 | 0.0006 | 0.0008 | 0.0012 | 0.0034 | 0.0008 |
None | 0.0038 | 0.0002 | 0.0004 | 0.001 | 0.0006 | 0.0001 | 0.0015 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.9022 | 0.8996 | 0.9236 | 0.908 | 0.9024 | 0.9653 | 0.9039 | 0.8861 | 0.9258 | 0.8976 | 0.9001 | 0.9668 | 0.963 | 0.9497 | 0.9513 | 0.9575 | 0.9588 | 0.9812 |
Nucleolus | 0.001 | 0.001 | 0.0036 | 0.0041 | 0.0028 | 0.0012 | 0.0008 | 0.0061 | 0.0017 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0028 | 0.0006 | 0.0005 | 0.0006 | 0.0003 |
Nucleus | 0.0042 | 0.0128 | 0.0088 | 0.0276 | 0.0233 | 0.0028 | 0.0037 | 0.0024 | 0.0085 | 0.0039 | 0.0014 | 0.001 | 0.0017 | 0.0057 | 0.0038 | 0.0023 | 0.0016 | 0.0013 |
Peroxisomes | 0.0011 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0012 | 0.0009 | 0.0001 | 0.0521 | 0.0347 | 0.007 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0048 | 0.0018 | 0.0125 | 0.0095 | 0.0018 | 0.0023 | 0.0177 | 0.0021 | 0.0014 | 0.0007 | 0.0007 | 0.0006 | 0.0005 | 0.0031 | 0.002 | 0.001 | 0.001 | 0.0008 |
Vacuole | 0.0011 | 0.0005 | 0.0006 | 0.0011 | 0.0009 | 0.0003 | 0.001 | 0.0009 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0359 | 0.0321 | 0.035 | 0.0303 | 0.036 | 0.0184 | 0.0358 | 0.0566 | 0.0452 | 0.0327 | 0.0252 | 0.0147 | 0.0295 | 0.033 | 0.0358 | 0.0318 | 0.0278 | 0.0126 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.9694 | 21.6252 | 26.8539 | 32.3119 | 26.5639 | 24.1776 | 28.3356 | 30.8705 | 30.1059 | 31.1292 |
Translational Efficiency | 1.6071 | 1.4054 | 1.0882 | 0.9036 | 1.0276 | 1.1344 | 1.0607 | 0.8299 | 0.8547 | 0.8968 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
437 | 1254 | 1576 | 1323 | 1686 | 1693 | 222 | 1426 | 2123 | 2947 | 1798 | 2749 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1249.16 | 1157.54 | 1321.82 | 1346.80 | 1052.04 | 1095.56 | 1409.80 | 1331.89 | 1092.62 | 1121.93 | 1332.68 | 1339.07 |
Standard Deviation | 177.17 | 235.71 | 199.26 | 248.80 | 206.14 | 336.51 | 190.93 | 238.66 | 215.77 | 299.39 | 200.35 | 243.71 |
Intensity Change Log 2 | — | -0.109896 | 0.081567 | 0.108577 | — | 0.058479 | 0.422301 | 0.340285 | — | -0.030475 | 0.247370 | 0.219141 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000958 | 0.000578 | 0.000338 | 0.000841 | 0.000294 | 0.000501 | 0.000177 | 0.000493 | 0.000430 | 0.000534 | 0.000318 | 0.000661 |
Bud Neck | 0.002718 | 0.000884 | 0.000868 | 0.007657 | 0.000396 | 0.000639 | 0.000564 | 0.006899 | 0.000874 | 0.000744 | 0.000830 | 0.007264 |
Bud Site | 0.000606 | 0.000145 | 0.000060 | 0.001344 | 0.000181 | 0.000238 | 0.000061 | 0.001385 | 0.000268 | 0.000198 | 0.000060 | 0.001366 |
Cell Periphery | 0.000067 | 0.000058 | 0.000019 | 0.000148 | 0.000048 | 0.000094 | 0.000016 | 0.000127 | 0.000052 | 0.000079 | 0.000019 | 0.000137 |
Cytoplasm | 0.000232 | 0.000482 | 0.000148 | 0.000474 | 0.000191 | 0.000963 | 0.000059 | 0.000903 | 0.000199 | 0.000758 | 0.000137 | 0.000697 |
Cytoplasmic Foci | 0.004555 | 0.002089 | 0.000201 | 0.009189 | 0.000583 | 0.001958 | 0.000964 | 0.009686 | 0.001401 | 0.002014 | 0.000295 | 0.009447 |
Eisosomes | 0.000017 | 0.000017 | 0.000005 | 0.000040 | 0.000009 | 0.000016 | 0.000006 | 0.000026 | 0.000010 | 0.000016 | 0.000005 | 0.000032 |
Endoplasmic Reticulum | 0.004686 | 0.003461 | 0.001132 | 0.001766 | 0.002559 | 0.003999 | 0.000750 | 0.001747 | 0.002997 | 0.003770 | 0.001085 | 0.001756 |
Endosome | 0.003503 | 0.001012 | 0.000252 | 0.003500 | 0.001348 | 0.001901 | 0.000159 | 0.004200 | 0.001791 | 0.001523 | 0.000240 | 0.003863 |
Golgi | 0.002323 | 0.000612 | 0.000115 | 0.003348 | 0.000432 | 0.000758 | 0.000173 | 0.003032 | 0.000821 | 0.000696 | 0.000123 | 0.003184 |
Lipid Particles | 0.004999 | 0.004388 | 0.000196 | 0.008480 | 0.002847 | 0.007431 | 0.000307 | 0.005531 | 0.003290 | 0.006136 | 0.000210 | 0.006951 |
Mitochondria | 0.005149 | 0.004395 | 0.001752 | 0.010310 | 0.004716 | 0.007117 | 0.000895 | 0.005611 | 0.004805 | 0.005959 | 0.001646 | 0.007873 |
Mitotic Spindle | 0.062634 | 0.003902 | 0.009129 | 0.051326 | 0.001093 | 0.002660 | 0.006467 | 0.067180 | 0.013761 | 0.003188 | 0.008800 | 0.059550 |
None | 0.000444 | 0.002325 | 0.000221 | 0.000680 | 0.000612 | 0.002060 | 0.000074 | 0.000622 | 0.000577 | 0.002173 | 0.000203 | 0.000650 |
Nuclear Periphery | 0.549859* | 0.558063* | 0.720551* | 0.468423* | 0.599909* | 0.581559* | 0.717158* | 0.456093* | 0.589607* | 0.571561* | 0.720133* | 0.462027* |
Nuclear Periphery Foci | 0.316660* | 0.349879* | 0.210546* | 0.331919* | 0.347172* | 0.334054* | 0.247161* | 0.333825* | 0.340892* | 0.340788* | 0.215067* | 0.332908* |
Nucleolus | 0.015988 | 0.031841 | 0.024737 | 0.048734 | 0.014079 | 0.022339 | 0.013258 | 0.052665 | 0.014472 | 0.026382 | 0.023320 | 0.050773 |
Nucleus | 0.008469 | 0.021952 | 0.025890 | 0.014203 | 0.011198 | 0.015380 | 0.008435 | 0.024450 | 0.010636 | 0.018177 | 0.023734 | 0.019519 |
Peroxisomes | 0.001183 | 0.001658 | 0.000463 | 0.002271 | 0.000515 | 0.000876 | 0.000516 | 0.002293 | 0.000653 | 0.001209 | 0.000470 | 0.002282 |
Vacuole | 0.007715 | 0.003118 | 0.000593 | 0.012722 | 0.003570 | 0.004415 | 0.000452 | 0.009614 | 0.004423 | 0.003863 | 0.000575 | 0.011110 |
Vacuole Periphery | 0.007234 | 0.009143 | 0.002785 | 0.022625 | 0.008249 | 0.011040 | 0.002347 | 0.013619 | 0.008040 | 0.010233 | 0.002731 | 0.017953 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.18 | 1.72 | 0.50 | -1.55 | -2.95 | -3.53 | 1.68 | -3.55 | -0.14 | -4.00 | -0.73 | 1.40 | -1.73 | -1.52 | -3.84 |
Bud Neck | 4.07 | 4.11 | -7.03 | -11.43 | -11.51 | -2.11 | -0.78 | -11.07 | -10.59 | -10.28 | 1.07 | 0.21 | -15.12 | -15.57 | -15.20 |
Bud Site | 2.32 | 2.65 | -2.34 | -5.47 | -5.80 | -0.57 | 1.22 | -6.75 | -6.61 | -7.75 | 0.95 | 2.65 | -7.19 | -8.40 | -9.53 |
Cell Periphery | 0.63 | 3.22 | -3.66 | -4.95 | -7.40 | -2.46 | 2.39 | -5.34 | -1.98 | -7.30 | -2.09 | 3.87 | -6.72 | -4.41 | -10.97 |
Cytoplasm | -1.66 | 1.69 | -2.81 | -0.46 | -4.46 | -2.88 | 1.78 | -4.82 | -0.20 | -5.91 | -3.30 | 1.13 | -5.48 | -0.20 | -6.85 |
Cytoplasmic Foci | 2.06 | 3.74 | -3.45 | -8.43 | -11.30 | -3.93 | -1.06 | -8.00 | -6.75 | -7.47 | -1.75 | 4.19 | -10.78 | -10.15 | -12.76 |
Eisosomes | 0.20 | 1.99 | -2.93 | -5.17 | -8.30 | -3.18 | 0.69 | -5.77 | -2.95 | -4.69 | -2.71 | 2.89 | -7.64 | -5.82 | -10.59 |
Endoplasmic Reticulum | 1.20 | 3.43 | 2.79 | 4.96 | -2.87 | -3.32 | 6.09 | 2.39 | 5.90 | -5.04 | -1.99 | 6.02 | 3.67 | 7.64 | -4.47 |
Endosome | 3.15 | 4.15 | -0.03 | -6.23 | -8.50 | -1.33 | 4.08 | -4.85 | -3.85 | -7.69 | 0.80 | 5.52 | -4.91 | -6.29 | -11.02 |
Golgi | 2.18 | 2.84 | -1.10 | -4.82 | -5.81 | -1.16 | 1.07 | -3.39 | -2.97 | -3.92 | 0.37 | 2.78 | -4.50 | -5.16 | -6.61 |
Lipid Particles | 0.41 | 3.31 | -1.96 | -3.32 | -8.37 | -4.24 | 5.43 | -2.87 | 1.47 | -6.33 | -3.53 | 6.50 | -4.62 | -0.93 | -10.54 |
Mitochondria | 0.37 | 1.62 | -2.11 | -4.20 | -6.39 | -1.94 | 3.84 | -0.99 | 1.08 | -5.11 | -1.19 | 3.90 | -2.99 | -2.16 | -8.03 |
Mitotic Spindle | 6.78 | 6.11 | 1.20 | -11.64 | -9.99 | -2.43 | -2.68 | -14.61 | -14.20 | -12.45 | 5.46 | 2.13 | -12.99 | -18.38 | -15.52 |
None | -2.06 | 0.70 | -0.81 | 1.87 | -4.58 | -3.18 | 4.47 | -0.33 | 3.11 | -5.88 | -3.51 | 3.13 | -0.82 | 3.34 | -6.17 |
Nuclear Periphery | -0.68 | -11.93 | 5.41 | 8.79 | 26.98 | 2.22 | -7.93 | 15.73 | 13.49 | 17.18 | 2.51 | -17.42 | 17.42 | 16.00 | 35.09* |
Nuclear Periphery Foci | -2.97 | 10.20 | -1.24 | 2.34 | -17.13 | 1.79 | 7.63 | 2.17 | 0.55 | -6.16 | 0.00 | 21.24 | 1.73 | 1.89 | -20.69 |
Nucleolus | -3.75 | -2.47 | -6.87 | -3.85 | -5.64 | -3.68 | 0.25 | -9.89 | -7.49 | -8.46 | -5.95 | -4.24 | -12.68 | -8.23 | -8.59 |
Nucleus | -5.10 | -6.33 | -3.11 | 2.63 | 4.01 | -2.05 | 0.94 | -6.01 | -4.03 | -5.32 | -4.39 | -5.52 | -5.92 | -1.37 | 1.33 |
Peroxisomes | -0.72 | 2.38 | -2.32 | -1.52 | -6.28 | -3.53 | -0.05 | -5.32 | -4.33 | -4.64 | -2.94 | 2.10 | -6.92 | -4.31 | -8.03 |
Vacuole | 2.19 | 3.34 | -2.01 | -8.85 | -11.65 | -0.95 | 4.90 | -5.10 | -4.42 | -8.78 | 0.80 | 5.72 | -6.80 | -8.86 | -14.25 |
Vacuole Periphery | -1.07 | 3.02 | -6.65 | -6.60 | -10.94 | -2.21 | 6.82 | -4.00 | -1.77 | -10.02 | -2.20 | 7.07 | -7.94 | -6.20 | -14.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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