Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.14 0.18 0 0.07 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.37 0.23 0.11 0.34 0.3 0.23 0.38 0.29 0.41 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.91 0.89 0.81 0.84 0.76 0.73 0.67 0.66 0.67 0.64 0.61 0.78 0.77 0.71 0.85 0.82 0.7 0.87 0.81 0.81 0.73 0.67 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.18 0.16 0.22 0.2 0.26 0.34 0.36 0.37 0.34 0.37 0.34 0.21 0.19 0.12 0.1 0.07 0.16 0.05 0.12 0.11 0.18 0.2 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Bud 1 0 1 0 1 1 1 10 3 2 9 4 0 1 1 0 0 0 5 3 2 7 5 6
Bud Neck 0 1 1 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 2 7 7 20 16 29 25 17 22 26 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 6 7 9 11 14 13 19 13 15 12 4 59 81 119 7 5 18 1 0 0 1 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 3 0 4 0 0 1 1 0 0
Endosome 1 1 0 1 0 0 0 1 0 0 0 1 4 3 7 2 0 0 5 1 5 4 5 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 1 3 1 1 7 5
Mitochondria 27 5 182 100 51 209 204 197 278 214 247 285 2 1 2 3 1 2 5 1 2 1 3 2
Nucleus 680 290 436 349 399 465 497 564 484 499 380 377 466 447 481 133 55 89 681 285 404 309 368 361
Nuclear Periphery 1 1 8 9 7 5 16 13 10 5 7 7 1 7 4 4 0 1 0 1 1 1 0 1
Nucleolus 92 56 77 96 96 156 228 302 271 256 221 208 122 109 84 15 5 20 40 40 57 75 107 114
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 1 2 8 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 2 2 1 1 1 6 6 10 0 0 0 5 1 2 4 5 8
Vac/Vac Membrane 2 3 2 0 9 5 3 9 4 8 7 6 7 7 14 6 3 6 29 9 13 15 39 46
Unique Cell Count 736 319 491 429 477 611 678 842 737 749 596 615 595 581 674 156 67 127 781 351 500 426 550 570
Labelled Cell Count 815 363 717 572 582 875 979 1146 1090 1018 908 919 672 665 731 173 72 140 781 351 500 426 550 570


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 9.3 4.8 5.7 6.4 5.1 5.4 5.8 5.3 5.8 5.3 5.4 8.9 9.1 9.1 11.4 11.4 10.6 8.9 9.5 9.5
Std Deviation (1e-4) 1.2 2.0 1.1 1.3 1.5 1.2 1.3 1.5 1.4 1.3 1.4 1.3 2.4 2.3 2.5 2.0 2.7 2.1 1.9 1.9 1.9
Intensity Change (Log2) – – – 0.23 0.41 0.09 0.17 0.26 0.13 0.25 0.14 0.16 0.88 0.91 0.91 1.24 1.24 1.14 0.89 0.97 0.98


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.4 2.7 3.5 3.5 2.6 3.7 4.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 1.0 1.0 0.6 1.1 0.5 0.8 0.7 0 5.8 7.4 8.8 0 0 6.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.5 -9.6 -1.0 -2.5 -5.3 0.2 -3.1 1.5 3.1 -16.1 -16.0 -17.0 -8.5 -5.8 -7.8
Nucleus -3.2 -2.3 -5.4 -6.5 -8.9 -9.2 -8.9 -9.5 -10.3 -4.6 -5.1 -7.2 -1.2 -1.6 -5.3
Nuclear Periphery 0.5 -0.2 -1.2 0.9 -0.1 -0.4 -1.6 -0.6 -0.7 0 -0.6 -1.7 0 0 0
Nucleolus 2.6 1.8 4.0 6.9 7.9 8.0 7.2 7.9 6.9 2.0 1.3 -1.6 -1.9 -1.8 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.2 0 0 0 0 0 0 0 0 0 2.4 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3297 4.1525 4.1863 3.6826 3.4477 4.2838 2.8454 2.5374 2.4342 2.4912 1.9218 2.6263 4.2994 4.2066 3.872 3.4836 3.4402 4.1084
Actin 0.0046 0 0.003 0 0 0 0.0241 0.0003 0.0011 0.0339 0.0042 0.0034 0.0003 0 0 0 0.0001 0
Bud 0.0003 0 0 0 0 0 0.0004 0 0.0005 0.0019 0.0007 0 0 0 0 0 0.0001 0
Bud Neck 0.0018 0.0001 0.0004 0 0.0003 0.0009 0.0008 0.0001 0.0008 0.0006 0.0064 0.0008 0.0001 0.0001 0 0.0001 0.0007 0.0009
Bud Periphery 0.0004 0 0 0 0 0 0.0009 0 0.0012 0.0033 0.0013 0.0001 0 0 0 0 0.0001 0
Bud Site 0.0009 0 0.0004 0 0 0.0001 0.002 0 0.0016 0.0046 0.0104 0.0001 0.0001 0 0 0 0.0001 0.0001
Cell Periphery 0.0002 0 0 0 0 0 0.0003 0 0.0006 0.0002 0.0003 0 0 0 0 0 0 0
Cytoplasm 0.0029 0.0003 0.0002 0 0 0.0008 0.0045 0.0001 0.002 0.0023 0.003 0.0008 0.0002 0.0002 0 0.0006 0.0001 0.0003
Cytoplasmic Foci 0.0038 0.0001 0.0001 0 0 0 0.0081 0.0013 0.002 0.0107 0.0362 0.0013 0.0043 0 0 0 0.0002 0
Eisosomes 0.0002 0 0 0 0 0 0.0002 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0012 0 0.0006 0 0 0 0.0042 0 0.0008 0.0013 0.0007 0.001 0.0001 0 0 0 0 0
Endosome 0.0031 0.0002 0.0007 0 0 0 0.0171 0.0006 0.0031 0.0058 0.0129 0.0041 0.0018 0 0 0 0.0001 0
Golgi 0.0008 0 0.0002 0 0 0 0.0019 0.0003 0.001 0.0033 0.0034 0.0011 0.0008 0 0 0 0.0001 0
Lipid Particles 0.0022 0 0.0002 0 0 0 0.0058 0.0013 0.0029 0.0048 0.0181 0.0034 0.012 0 0 0 0.0002 0
Mitochondria 0.0034 0 0.0001 0 0.0001 0.0001 0.0027 0.0001 0.0026 0.007 0.0028 0.0013 0.0002 0.0001 0 0.0001 0.0003 0.0001
None 0.0005 0 0.0001 0 0 0 0.0031 0.0001 0.0025 0.0032 0.0047 0.0003 0.0002 0 0 0 0.0001 0
Nuclear Periphery 0.0075 0.0008 0.0015 0.0002 0.0006 0.0005 0.0306 0.0014 0.0058 0.0054 0.0062 0.0155 0.0012 0.0065 0.0001 0.0009 0.0011 0.0005
Nucleolus 0.0268 0.024 0.019 0.006 0.0661 0.0692 0.0318 0.0263 0.0179 0.0229 0.104 0.0738 0.0208 0.0189 0.0249 0.0192 0.0658 0.0768
Nucleus 0.928 0.9667 0.9725 0.9936 0.93 0.9258 0.8413 0.9608 0.9437 0.8462 0.7111 0.8797 0.9381 0.9726 0.9745 0.9767 0.8624 0.9193
Peroxisomes 0.0004 0 0 0 0 0 0.0034 0.0021 0.0007 0.0038 0.0493 0.0026 0.0105 0 0 0 0 0
Punctate Nuclear 0.0079 0.0066 0.0006 0.0002 0.0027 0.0024 0.0104 0.0051 0.0067 0.037 0.0197 0.0097 0.0092 0.0013 0.0003 0.0021 0.0685 0.0017
Vacuole 0.0021 0.0009 0.0001 0 0 0.0001 0.0038 0 0.0022 0.0008 0.0038 0.0003 0.0001 0.0001 0 0 0.0001 0.0001
Vacuole Periphery 0.001 0.0001 0 0 0 0 0.0024 0.0001 0.0003 0.0008 0.0008 0.0005 0.0001 0.0001 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.6846 26.9502 35.9967 27.3559 35.179 9.0393 29.293 32.185 44.6408 30.1326
Translational Efficiency 0.6122 0.9608 0.651 1.253 0.8392 2.1368 0.855 1.057 0.6855 0.7841

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
213 1100 147 1037 1811 1594 2085 338 2024 2694 2232 1375

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 783.50 881.96 1132.91 964.89 804.05 924.90 951.62 1045.45 801.89 907.37 963.56 984.69
Standard Deviation 115.95 190.96 135.33 154.52 127.18 140.09 147.46 149.82 126.20 164.15 153.43 157.25
Intensity Change Log 2 — 0.170780 0.532028 0.300431 — 0.202012 0.243100 0.378767 — 0.186683 0.392920 0.340637

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000568 0.000931 0.001806 0.001556 0.000346 0.000647 0.000895 0.003586 0.000369 0.000763 0.000955 0.002055
Bud Neck 0.003950 0.007860 0.009811 0.022538 0.006193 0.007206 0.029564 0.026936 0.005957 0.007473 0.028263 0.023619
Bud Site 0.000672 0.001321 0.001637 0.003713 0.000755 0.000859 0.002469 0.008684 0.000746 0.001047 0.002415 0.004935
Cell Periphery 0.000039 0.000150 0.000051 0.000105 0.000041 0.000047 0.000097 0.000509 0.000041 0.000089 0.000094 0.000204
Cytoplasm 0.000471 0.002446 0.000242 0.004948 0.001311 0.000427 0.001625 0.004952 0.001223 0.001252 0.001534 0.004949
Cytoplasmic Foci 0.000049 0.000260 0.000268 0.000822 0.000147 0.000435 0.000371 0.002233 0.000136 0.000363 0.000364 0.001169
Eisosomes 0.000031 0.000038 0.000075 0.000040 0.000025 0.000038 0.000043 0.000047 0.000026 0.000038 0.000045 0.000042
Endoplasmic Reticulum 0.002751 0.002914 0.004757 0.004089 0.001966 0.002339 0.002744 0.005010 0.002048 0.002574 0.002877 0.004315
Endosome 0.000112 0.000211 0.000141 0.001677 0.000128 0.000590 0.000709 0.003168 0.000126 0.000435 0.000672 0.002043
Golgi 0.000104 0.000198 0.000220 0.002977 0.000121 0.000419 0.000296 0.006353 0.000119 0.000329 0.000291 0.003807
Lipid Particles 0.000130 0.000114 0.000305 0.000203 0.000081 0.000290 0.000172 0.000231 0.000086 0.000218 0.000181 0.000210
Mitochondria 0.000700 0.001519 0.001294 0.003589 0.000616 0.002531 0.002666 0.003054 0.000624 0.002118 0.002575 0.003458
Mitotic Spindle 0.007227 0.003484 0.000582 0.023225 0.001590 0.004294 0.005877 0.023204 0.002183 0.003964 0.005528 0.023220
None 0.002383 0.013215 0.001169 0.010158 0.002739 0.001258 0.004235 0.011206 0.002702 0.006140 0.004033 0.010416
Nuclear Periphery 0.000142 0.000220 0.000155 0.001020 0.000169 0.000626 0.000509 0.000819 0.000166 0.000460 0.000485 0.000971
Nuclear Periphery Foci 0.000276 0.000775 0.000151 0.001111 0.000541 0.001213 0.000322 0.000367 0.000513 0.001034 0.000311 0.000928
Nucleolus 0.108473 0.100602 0.081604 0.061703 0.092264 0.120478 0.071862 0.031800 0.093970 0.112362 0.072504 0.054352
Nucleus 0.870965* 0.862185* 0.891318* 0.849529* 0.890260* 0.853535* 0.873728* 0.862659* 0.888230* 0.857067* 0.874886* 0.852756*
Peroxisomes 0.000744 0.001083 0.002695 0.000833 0.000372 0.001300 0.000682 0.000500 0.000411 0.001211 0.000815 0.000751
Vacuole 0.000119 0.000268 0.001480 0.003721 0.000176 0.000633 0.000837 0.004001 0.000170 0.000484 0.000879 0.003790
Vacuole Periphery 0.000092 0.000204 0.000239 0.002442 0.000159 0.000837 0.000297 0.000680 0.000152 0.000579 0.000294 0.002009

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.23 -8.00 -8.45 -5.60 0.98 -4.31 -15.71 -4.63 -4.07 -3.50 -5.61 -16.89 -10.73 -7.91 -6.59
Bud Neck -4.07 -6.07 -13.48 -12.08 -8.25 -1.54 -21.23 -11.66 -11.03 -1.22 -2.93 -21.64 -17.33 -15.92 1.17
Bud Site -1.44 -3.81 -5.45 -4.70 -3.34 -0.20 -3.88 -4.83 -4.88 -3.52 -0.80 -4.21 -6.89 -7.10 -4.10
Cell Periphery -2.65 -1.48 -4.18 1.18 -4.08 -0.47 -3.68 -1.86 -1.82 -1.49 -2.63 -3.85 -3.38 -1.53 -1.94
Cytoplasm -2.55 0.77 -5.02 -2.59 -5.34 2.04 0.09 -3.13 -4.81 -3.56 0.10 0.04 -4.54 -4.76 -4.94
Cytoplasmic Foci -1.87 -1.19 -2.68 -0.72 -0.83 -1.10 -0.29 -1.03 -0.52 -0.92 -1.34 -0.44 -1.85 -0.66 -1.35
Eisosomes -2.18 -7.51 -4.88 -3.25 5.45 -6.88 -13.80 -8.33 -5.17 -3.51 -7.97 -15.21 -12.88 -6.03 -1.49
Endoplasmic Reticulum -0.38 -3.49 -4.38 -7.05 0.36 -1.84 -4.48 -7.01 -6.48 -5.90 -3.22 -5.01 -12.73 -10.66 -9.01
Endosome -1.84 -1.78 -3.04 -2.70 -2.83 -2.96 -1.92 -1.86 -1.16 -1.20 -3.25 -1.94 -3.53 -2.40 -1.88
Golgi -2.18 -5.01 -1.92 -1.78 -1.69 -2.20 -2.38 -1.38 -1.17 -1.27 -2.55 -2.59 -2.33 -2.00 -2.10
Lipid Particles -0.15 -1.46 -1.76 -2.27 0.72 -2.19 -1.11 -4.19 -0.99 -3.12 -2.34 -1.36 -5.63 -0.87 -2.58
Mitochondria -1.84 -5.81 -6.96 -3.49 -4.79 -2.83 -7.78 -7.71 -2.38 -3.29 -3.40 -8.00 -9.66 -3.11 -2.98
Mitotic Spindle 0.60 1.02 -3.50 -6.04 -6.85 -1.98 -3.42 -5.60 -5.29 -5.12 -1.54 -2.70 -8.59 -8.08 -7.65
None -4.64 1.65 -3.19 2.09 -4.33 3.62 -0.97 -3.23 -4.96 -2.73 -3.06 -0.91 -3.65 -0.94 -3.19
Nuclear Periphery -1.42 -1.80 -11.09 -10.15 -10.34 -2.10 -5.14 -7.92 -4.63 -6.22 -2.23 -5.27 -13.38 -7.19 -9.10
Nuclear Periphery Foci -1.61 0.32 -2.36 -0.77 -2.63 -1.11 1.05 0.05 1.53 -1.93 -1.16 1.05 -1.26 -0.28 -2.61
Nucleolus 0.83 1.75 4.99 7.61 2.73 -6.49 2.21 11.20 16.18 9.25 -4.90 2.69 9.94 15.19 7.14
Nucleus 0.83 -0.51 2.85 3.06 3.59 6.87 6.34 5.73 2.96 3.32 6.91 5.96 8.43 3.90 4.17
Peroxisomes -1.52 -4.48 -0.80 1.16 4.32 -2.99 -3.23 -3.99 1.90 -0.11 -3.87 -4.60 -4.95 1.96 0.49
Vacuole -3.07 -4.36 -5.26 -5.13 -3.13 -2.65 -4.53 -3.84 -3.69 -3.62 -2.91 -5.47 -6.46 -6.17 -5.76
Vacuole Periphery -3.06 -5.10 -2.07 -1.89 -1.77 -2.54 -3.54 -2.89 1.24 -1.69 -2.68 -4.02 -2.14 -1.17 -1.85
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Srb6

Srb6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available