Standard name
Human Ortholog
Description Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.94 0.99 0.95 0.85 0.76 0.72 – 0.66 0.65 0.63 0.97 0.97 0.98 0.98 0.98 0.98 0.9 0.9 0.81 0.8 0.85 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0.1 0.34 0.43 0.46 – 0.46 0.6 0.62 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 2 3 2 0 0 2
Bud 1 0 0 0 1 5 8 12 – 7 10 6 0 1 0 0 0 1 0 3 1 1 4 3
Bud Neck 0 0 1 0 0 2 0 1 – 0 3 1 0 0 1 0 0 0 2 1 0 0 1 0
Bud Site 0 0 0 0 0 1 0 1 – 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 3 2 2 4 3 3 – 2 2 4 0 0 0 10 7 17 0 2 2 0 0 0
Cytoplasm 434 417 219 229 313 343 392 387 – 257 183 239 157 213 231 318 262 232 418 382 195 100 165 191
Endoplasmic Reticulum 2 4 0 0 1 1 2 2 – 1 0 1 3 1 2 11 9 8 1 1 1 2 2 1
Endosome 0 0 2 0 0 1 1 7 – 1 1 2 1 1 2 0 1 1 5 2 4 1 1 5
Golgi 3 1 1 1 0 0 0 0 – 0 0 0 0 1 0 1 0 1 7 0 0 4 1 0
Mitochondria 4 3 13 8 34 138 225 246 – 178 169 235 0 1 0 6 2 2 10 11 22 5 7 7
Nucleus 1 1 0 0 1 2 3 5 – 5 0 3 1 0 1 1 1 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 3 1 – 2 0 0 0 0 0 0 1 0 0 0 1 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 2 3 4 – 5 4 3 0 1 0 0 0 0 4 1 1 0 1 3
Vac/Vac Membrane 1 3 0 1 6 1 6 10 – 16 2 1 0 1 0 3 4 5 7 5 2 3 2 4
Unique Cell Count 442 424 232 232 329 403 518 534 388 282 379 162 219 236 325 268 237 466 424 242 126 194 229
Labelled Cell Count 447 433 241 241 359 500 646 680 475 375 495 163 221 238 350 287 267 466 424 242 126 194 229


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.6 3.9 4.1 3.9 3.6 3.3 3.4 – 3.2 3.4 3.1 5.1 5.1 5.1 6.5 6.4 6.6 6.2 5.9 6.3
Std Deviation (1e-4) 0.8 0.8 1.3 1.9 1.6 1.5 1.4 1.3 – 1.1 1.6 1.2 0.9 1.1 1.3 1.1 1.4 1.5 2.5 1.6 1.9
Intensity Change (Log2) – – – 0.04 -0.01 -0.13 -0.25 -0.23 – -0.32 -0.22 -0.34 0.36 0.36 0.37 0.73 0.7 0.75 0.65 0.57 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4795 0.9176 0.7209 0.5525 0.8694 0.8283 2.5239 2.6464 2.5102 1.9999 2.0036 2.7498 -0.4988 0.1243 -0.1009 -0.0042 -0.0169 -0.0928
Actin 0.0013 0.0026 0.0011 0.0027 0.0032 0.0024 0.0474 0.0001 0.0121 0.0004 0.0041 0.0064 0.0008 0.0024 0.0004 0.0001 0.0001 0.0006
Bud 0.0001 0.0017 0.0003 0.0001 0.0034 0.0002 0.0007 0.0001 0.0014 0.005 0.0048 0.0012 0.0001 0.0012 0.0004 0.0001 0 0.0002
Bud Neck 0.0004 0.0005 0.0001 0.0001 0.0016 0.0009 0.0041 0.0002 0.0027 0.0001 0.0007 0.0006 0.0003 0.0003 0.0001 0.0001 0.0001 0.0003
Bud Periphery 0.0001 0.001 0.0004 0.0002 0.0051 0.0005 0.0014 0.0001 0.0021 0.0154 0.0252 0.0031 0.0001 0.0019 0.0003 0.0001 0 0.0003
Bud Site 0.0009 0.0129 0.0008 0.0001 0.0052 0.0001 0.0049 0.0023 0.003 0.0001 0.0034 0.0002 0.0006 0.0107 0.0003 0.0001 0 0
Cell Periphery 0.0001 0.0003 0.0001 0 0.0002 0.0001 0.0004 0.0001 0.0002 0.0001 0.0005 0 0.0001 0.0002 0.0001 0 0 0
Cytoplasm 0.1766 0.2957 0.2283 0.2424 0.2951 0.2731 0.1455 0.3226 0.1946 0.1659 0.3332 0.2393 0.1977 0.312 0.2529 0.4412 0.2489 0.3727
Cytoplasmic Foci 0.006 0.0075 0.0056 0.0054 0.0355 0.0064 0.0063 0.0072 0.0158 0.0014 0.0157 0.0094 0.0106 0.0049 0.0063 0.009 0.003 0.0057
Eisosomes 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0021 0.0021 0.002 0.0011 0.0025 0.001 0.0017 0.0011 0.0009 0.0007 0.0012 0.0014 0.0011 0.0017 0.0015 0.001 0.0003 0.0005
Endosome 0.002 0.004 0.0018 0.0022 0.0119 0.002 0.0079 0.0021 0.0029 0.0004 0.0052 0.0013 0.0038 0.0013 0.0032 0.0025 0.0004 0.001
Golgi 0.0005 0.0004 0.0002 0.0002 0.0084 0.0003 0.0022 0.0002 0.0016 0 0.0037 0.0002 0.0003 0.0002 0.0002 0.0002 0 0
Lipid Particles 0.0011 0.0003 0.0002 0.0001 0.0028 0.0004 0.0021 0.0001 0.0015 0 0.0015 0.0001 0.0008 0.0001 0.0002 0 0 0
Mitochondria 0.0005 0.0003 0.0003 0.0004 0.0103 0.0003 0.0074 0.0003 0.0007 0.0011 0.0033 0.0003 0.0003 0.0003 0.0003 0.0004 0.0001 0.0001
None 0.7951 0.6649 0.7539 0.7404 0.6012 0.7088 0.7451 0.6593 0.7494 0.7988 0.5785 0.732 0.7761 0.6567 0.7257 0.5419 0.7449 0.6162
Nuclear Periphery 0.0048 0.0008 0.0008 0.0008 0.001 0.0005 0.002 0.0008 0.0005 0.0021 0.0009 0.0008 0.0012 0.0016 0.0007 0.0004 0.0002 0.0002
Nucleolus 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0012 0.0001 0.0003 0.0005 0.0008 0 0.0002 0.0002 0.0002 0 0 0
Nucleus 0.0036 0.0022 0.0014 0.0015 0.0018 0.0011 0.0078 0.0012 0.0008 0.0046 0.0015 0.0009 0.0013 0.0018 0.003 0.0015 0.0008 0.0008
Peroxisomes 0.0029 0.0005 0.0002 0.0004 0.0054 0.0002 0.0046 0.0001 0.006 0.0001 0.0106 0.0017 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0006 0.0011 0.0016 0.0008 0.0029 0.0011 0.002 0.0008 0.0027 0.0004 0.0018 0.0005 0.0034 0.0011 0.0032 0.0006 0.0006 0.0008
Vacuole 0.0009 0.0008 0.0005 0.0007 0.0019 0.0004 0.0046 0.001 0.0006 0.0016 0.0025 0.0003 0.0005 0.0007 0.0007 0.0004 0.0001 0.0002
Vacuole Periphery 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0005 0.0002 0.0001 0.0008 0.0007 0.0001 0.0001 0.0002 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.3096 16.4396 9.7591 20.4871 20.267 18.3799 23.6864 26.3641 22.2532 22.3895
Translational Efficiency 1.6025 1.7649 2.2724 1.15 1.6591 1.4813 1.4136 0.9729 1.0567 1.3433

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
109 644 2479 392 1821 1340 2450 799 1930 1984 4929 1191

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 567.54 659.25 796.66 872.69 685.08 666.30 801.42 848.11 678.44 664.01 799.03 856.20
Standard Deviation 57.59 103.85 104.30 119.37 84.85 99.82 104.68 118.61 87.84 101.20 104.52 119.42
Intensity Change Log 2 — 0.216104 0.489242 0.620747 — -0.040101 0.226286 0.307979 — 0.081642 0.351391 0.458131

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000124 0.000293 0.000128 0.000351 0.000644 0.000475 0.001495 0.002666 0.000615 0.000416 0.000807 0.001904
Bud Neck 0.017581 0.021212 0.006575 0.012711 0.012714 0.022136 0.011429 0.007747 0.012989 0.021836 0.008987 0.009381
Bud Site 0.002608 0.012089 0.006247 0.044287 0.004014 0.009682 0.023583 0.029814 0.003935 0.010463 0.014864 0.034578
Cell Periphery 0.000368 0.000171 0.000086 0.000161 0.000137 0.000207 0.000135 0.000205 0.000150 0.000196 0.000110 0.000191
Cytoplasm 0.358498* 0.460289* 0.313491* 0.478640* 0.558687* 0.444988* 0.589738* 0.477170* 0.547381* 0.449955* 0.450802* 0.477654*
Cytoplasmic Foci 0.192722* 0.182841* 0.002941 0.016370 0.136206 0.257888* 0.009964 0.033151 0.139398 0.233528* 0.006432 0.027628
Eisosomes 0.000215 0.000113 0.000013 0.000056 0.000100 0.000149 0.000022 0.000068 0.000106 0.000137 0.000017 0.000064
Endoplasmic Reticulum 0.000628 0.000878 0.001727 0.003357 0.000404 0.000660 0.000906 0.002019 0.000417 0.000731 0.001319 0.002459
Endosome 0.003961 0.016697 0.000684 0.003757 0.008188 0.020429 0.001723 0.005410 0.007949 0.019218 0.001200 0.004866
Golgi 0.000888 0.003249 0.000080 0.000207 0.001405 0.004504 0.000405 0.001080 0.001376 0.004097 0.000242 0.000793
Lipid Particles 0.014460 0.007706 0.000405 0.001409 0.004262 0.011725 0.000516 0.001925 0.004838 0.010420 0.000460 0.001756
Mitochondria 0.008028 0.008860 0.000814 0.000727 0.003172 0.013362 0.001529 0.002204 0.003446 0.011901 0.001169 0.001717
Mitotic Spindle 0.000531 0.007932 0.006097 0.023485 0.003528 0.004100 0.019906 0.017987 0.003359 0.005344 0.012961 0.019796
None 0.008628 0.013590 0.006178 0.011674 0.004066 0.004735 0.004947 0.005010 0.004324 0.007609 0.005566 0.007203
Nuclear Periphery 0.001063 0.000608 0.001798 0.001382 0.000385 0.000546 0.001196 0.001105 0.000423 0.000566 0.001499 0.001196
Nuclear Periphery Foci 0.000414 0.000760 0.000315 0.001826 0.000170 0.000427 0.000307 0.001064 0.000184 0.000535 0.000311 0.001314
Nucleolus 0.002592 0.002468 0.000390 0.000326 0.001038 0.001906 0.000499 0.000469 0.001126 0.002088 0.000444 0.000422
Nucleus 0.363126* 0.185480* 0.637430* 0.231006* 0.202663* 0.134475* 0.280322* 0.285556* 0.211725* 0.151031* 0.459926* 0.267602*
Peroxisomes 0.010193 0.012598 0.000152 0.000443 0.002457 0.010402 0.001517 0.001156 0.002894 0.011115 0.000830 0.000921
Vacuole 0.012652 0.060731 0.014203 0.167509 0.054762 0.054921 0.048662 0.123556 0.052383 0.056807 0.031331 0.138023
Vacuole Periphery 0.000721 0.001434 0.000246 0.000318 0.000998 0.002283 0.001200 0.000639 0.000983 0.002007 0.000720 0.000533

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.81 -1.03 -4.76 -2.24 -5.51 0.29 -0.53 -1.16 -1.87 -0.82 0.37 0.06 -0.90 -1.99 -1.46
Bud Neck -0.55 2.11 2.23 4.33 0.61 -4.62 2.37 3.77 7.26 2.01 -4.69 4.07 4.67 8.41 1.68
Bud Site -4.27 -2.47 -3.36 -0.82 -2.58 -4.65 -6.74 -4.82 -2.81 -0.88 -5.76 -6.13 -5.66 -2.80 -2.80
Cell Periphery 1.68 2.38 1.98 1.92 -3.90 -2.78 1.43 -1.31 1.90 -3.19 -2.11 3.01 -0.18 2.45 -5.02
Cytoplasm -3.49 2.86 1.30 7.37 -2.43 11.69 6.15 14.66 4.49 10.43 11.33 20.72 17.94 7.90 3.22
Cytoplasmic Foci 0.31 9.66 9.12 21.21 -7.31 -16.81 32.03 25.58 36.61 -8.53 -15.12 34.36 28.86 41.81 -11.42
Eisosomes 3.80 7.64 5.20 4.33 -14.17 -6.55 14.35 3.65 10.16 -11.92 -4.82 17.01 5.03 10.99 -17.83
Endoplasmic Reticulum -2.04 -12.40 -10.41 -9.21 -5.31 -2.83 -13.61 -12.60 -11.16 -7.55 -4.18 -19.85 -16.60 -14.43 -8.18
Endosome -5.88 4.01 1.21 6.75 -7.95 -7.40 9.07 4.27 10.06 -2.88 -8.08 9.82 5.24 12.09 -4.38
Golgi -3.68 4.53 4.19 5.12 -2.46 -4.90 2.98 1.77 6.66 -0.85 -5.06 3.35 2.33 8.22 -1.09
Lipid Particles 2.90 6.25 5.65 7.51 -10.05 -7.31 8.60 5.23 10.25 -9.75 -7.03 10.16 6.79 12.24 -13.27
Mitochondria -0.14 3.08 3.21 4.36 3.20 -5.27 2.29 2.17 6.68 0.12 -5.44 3.09 3.01 7.91 0.22
Mitotic Spindle -3.23 -5.52 -4.05 -2.32 -2.90 -0.42 -5.69 -3.51 -3.29 -0.02 -1.48 -5.24 -4.83 -4.02 -2.28
None -1.12 1.05 0.88 2.36 -0.36 -0.71 -1.22 0.17 0.83 1.35 -2.54 -1.53 -0.37 2.35 1.16
Nuclear Periphery 1.44 -4.39 -5.14 -12.27 -2.30 -2.00 -23.03 -11.24 -9.20 -0.96 -2.38 -32.46 -16.18 -13.74 -1.27
Nuclear Periphery Foci -1.95 -0.32 -6.84 -5.67 -8.60 -3.15 -5.06 -9.28 -6.69 -7.00 -5.15 -7.33 -13.17 -8.76 -10.76
Nucleolus 0.14 4.12 4.15 4.82 0.37 -2.64 4.01 4.50 4.39 0.15 -3.48 5.43 5.58 6.18 0.19
Nucleus 5.76 -9.36 3.14 -5.05 28.15 9.88 -19.68 -8.79 -15.32 4.84 9.12 -43.97 -8.07 -15.27 25.44
Peroxisomes -0.71 3.16 3.13 7.21 -1.20 -9.46 3.66 6.82 11.89 0.68 -9.95 7.07 8.02 13.53 0.05
Vacuole -10.97 -5.08 -20.42 -16.34 -19.74 0.07 -8.23 -18.48 -18.34 -14.82 -1.86 -0.94 -25.04 -24.25 -25.11
Vacuole Periphery -2.34 2.64 3.05 5.37 0.37 -3.92 0.88 2.39 6.99 1.21 -3.64 2.08 2.89 8.46 0.98
Standard name
Human Ortholog
Description Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ypt10

Ypt10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypt10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available