Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.08 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.97 0.98 0.88 0.77 0.73 – 0.8 0.68 0.7 0.99 0.99 1.0 0.99 0.99 0.99 0.93 0.96 0.96 0.89 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.11 0.14 0.32 0.47 0.53 – 0.45 0.56 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 0 0 0 0 1 8 11 7 – 3 8 14 0 0 0 0 1 0 2 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 1 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 – 1 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 2 0 2 – 3 1 4 4 3 8 30 27 9 0 0 0 0 0 0
Cytoplasm 128 90 151 205 292 309 321 442 – 397 218 283 293 454 501 310 351 227 125 97 149 214 250 294
Endoplasmic Reticulum 1 1 2 1 0 1 1 0 – 0 0 1 2 0 0 15 11 13 0 0 0 3 4 4
Endosome 0 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 1 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 2 1 19 24 41 112 195 324 – 224 180 222 0 0 0 3 1 0 1 0 1 5 2 4
Nucleus 0 0 1 0 0 2 3 4 – 2 0 0 3 2 0 0 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 1 0 0 0 3 1 – 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 – 4 3 3 0 1 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 0 0 0 1 0 0 4 4 – 4 4 0 0 1 2 1 1 1 0 0 0 11 5 8
Unique Cell Count 130 93 153 211 299 350 415 608 498 321 407 297 457 501 313 355 229 136 102 155 242 271 327
Labelled Cell Count 131 93 175 232 335 434 540 789 639 416 539 302 461 511 360 393 251 136 102 155 242 271 327


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.6 4.4 4.8 4.3 4.0 3.3 3.3 – 3.0 2.9 3.0 6.6 6.8 6.8 6.6 6.4 6.7 7.1 7.4 7.6
Std Deviation (1e-4) 0.8 1.1 0.5 1.4 0.8 1.3 1.1 0.9 – 1.0 1.1 1.2 0.8 1.3 1.1 1.0 1.0 1.0 1.2 1.7 1.4
Intensity Change (Log2) – – – 0.14 -0.0 -0.11 -0.39 -0.42 – -0.52 -0.58 -0.54 0.6 0.65 0.64 0.6 0.57 0.63 0.7 0.77 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 3.6 3.1 0
Cytoplasm -1.0 -0.7 -3.8 -6.0 -6.9 – -5.6 -7.5 -7.4 0 0.8 2.6 0.3 0.2 0.4
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 1.9 0 2.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -6.2 0 0 -5.5 -6.5 -5.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1518 1.7635 1.6398 1.1239 1.0159 1.7129 1.9147 3.2981 2.5352 2.6594 2.5111 2.7857 -0.132 0.1951 -0.2724 -0.1354 -0.2529 -0.2334
Actin 0.0066 0.0016 0.0022 0.0001 0.011 0.0014 0.0034 0.0003 0.0017 0.0007 0.0004 0.0008 0.0194 0.0001 0.0001 0.0002 0.007 0.0001
Bud 0.002 0.0012 0.0017 0.0003 0.0008 0.0005 0.0002 0.0003 0.0004 0.0003 0.0002 0.0001 0.0006 0.0001 0.0001 0.0002 0.0003 0
Bud Neck 0.0019 0.0004 0.0014 0.0002 0.0004 0.001 0.0005 0.0001 0.0006 0.0002 0.0003 0.0003 0.003 0 0 0.0001 0.0003 0.0003
Bud Periphery 0.0016 0.0012 0.0013 0.0001 0.0014 0.0003 0.0002 0.0002 0.0004 0.0005 0.0002 0.0001 0.0007 0 0 0.0002 0.0002 0
Bud Site 0.0054 0.0045 0.0075 0.0001 0.0009 0.0001 0.0005 0.002 0.002 0.0002 0.0002 0 0.0158 0.0003 0.0002 0.0001 0.0006 0
Cell Periphery 0.0003 0.0002 0.0002 0 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.4795 0.7172 0.5806 0.6992 0.5548 0.6814 0.5418 0.646 0.5813 0.5757 0.6169 0.6447 0.3674 0.6691 0.7878 0.64 0.5292 0.8913
Cytoplasmic Foci 0.023 0.0149 0.015 0.0164 0.013 0.015 0.0164 0.0084 0.0143 0.01 0.0349 0.0071 0.0311 0.0071 0.0047 0.0093 0.0309 0.0033
Eisosomes 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0005 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0038 0.0017 0.0024 0.0018 0.0022 0.0029 0.0035 0.0016 0.0039 0.0015 0.0018 0.0009 0.0135 0.0041 0.0022 0.0029 0.0017 0.0015
Endosome 0.0127 0.006 0.0104 0.0161 0.0064 0.0078 0.0092 0.0042 0.0089 0.0064 0.011 0.0044 0.0329 0.0033 0.0033 0.0156 0.1042 0.0016
Golgi 0.0014 0.0006 0.001 0.0003 0.0007 0.0008 0.0014 0.0004 0.0008 0.0003 0.0005 0.0007 0.0192 0.0002 0.0002 0.0005 0.0369 0.0001
Lipid Particles 0.0012 0.0003 0.0003 0.0001 0.0003 0.0001 0.002 0.0001 0.0004 0.0003 0.0002 0.0024 0.0596 0.0003 0.0001 0.0002 0.0128 0
Mitochondria 0.0007 0.0004 0.0012 0.0004 0.0007 0.0017 0.0005 0.0003 0.0006 0.0008 0.0003 0.0004 0.0077 0.0002 0.0001 0.0002 0.0549 0.0001
None 0.447 0.2436 0.367 0.2586 0.4023 0.2815 0.4105 0.3316 0.3739 0.3977 0.3299 0.3347 0.3693 0.3105 0.198 0.3169 0.1195 0.1004
Nuclear Periphery 0.0011 0.0006 0.0009 0.0009 0.0007 0.0005 0.0015 0.0003 0.002 0.0005 0.0003 0.0002 0.0246 0.0009 0.0006 0.0012 0.0005 0.0002
Nucleolus 0.0006 0.0002 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0079 0 0 0.0001 0.0002 0
Nucleus 0.0021 0.0017 0.0018 0.0015 0.0012 0.0013 0.0008 0.0007 0.0025 0.0008 0.0005 0.0004 0.0044 0.0006 0.0004 0.0006 0.0005 0.0003
Peroxisomes 0.0025 0.0004 0.0005 0.0001 0.0006 0.0003 0.0017 0.0001 0.0008 0.0002 0.0006 0.0016 0.0087 0 0 0.0001 0.0933 0
Punctate Nuclear 0.0032 0.0009 0.001 0.0004 0.0006 0.0018 0.003 0.0003 0.0018 0.0003 0.0004 0.0002 0.0061 0.0002 0.0001 0.0005 0.0004 0
Vacuole 0.0029 0.002 0.0024 0.0027 0.0013 0.0011 0.0021 0.0026 0.0027 0.0027 0.0011 0.0008 0.0043 0.0024 0.0017 0.0093 0.0046 0.0005
Vacuole Periphery 0.0004 0.0003 0.0005 0.0006 0.0003 0.0002 0.0003 0.0002 0.0004 0.0005 0.0002 0.0002 0.0025 0.0002 0.0002 0.0019 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7936 12.9876 8.6229 11.4382 11.4013 12.2278 14.1685 10.729 15.0968 15.6515
Translational Efficiency 1.5009 1.1171 1.2391 1.2753 1.1935 1.2139 1.3136 1.1573 1.1103 1.1081

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1975 1826 107 162 576 1757 215 1568 2551 3583 322 1730

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 737.43 860.25 1178.37 1155.60 812.15 814.15 1115.13 1088.41 754.30 837.64 1136.14 1094.70
Standard Deviation 92.37 126.88 133.96 157.51 105.71 106.14 126.30 153.07 100.53 119.42 132.30 154.73
Intensity Change Log 2 — 0.222250 0.676215 0.648064 — 0.003548 0.457394 0.422404 — 0.111767 0.565674 0.534202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.000226 0.000530 0.000330 0.000242 0.000383 0.000744 0.000674 0.000129 0.000303 0.000673 0.000642
Bud Neck 0.008222 0.007457 0.001175 0.001824 0.007180 0.014586 0.001209 0.001326 0.007987 0.010953 0.001198 0.001373
Bud Site 0.007049 0.017847 0.010101 0.011379 0.008179 0.024121 0.015933 0.016951 0.007304 0.020924 0.013995 0.016430
Cell Periphery 0.000085 0.000106 0.000181 0.000076 0.000182 0.000095 0.000255 0.000058 0.000107 0.000101 0.000231 0.000060
Cytoplasm 0.624022* 0.639343* 0.777882* 0.485055* 0.558027* 0.535972* 0.762230* 0.819389* 0.609121* 0.588653* 0.767431* 0.788081*
Cytoplasmic Foci 0.201482* 0.192723* 0.065580 0.066229 0.275782* 0.232220* 0.054079 0.052115 0.218259* 0.212091* 0.057901 0.053436
Eisosomes 0.000070 0.000054 0.000019 0.000033 0.000130 0.000081 0.000023 0.000010 0.000084 0.000067 0.000022 0.000012
Endoplasmic Reticulum 0.000556 0.000867 0.001188 0.000417 0.001162 0.000906 0.001144 0.000127 0.000693 0.000886 0.001159 0.000154
Endosome 0.015117 0.016137 0.004216 0.005466 0.024456 0.029296 0.004268 0.003054 0.017225 0.022590 0.004251 0.003280
Golgi 0.004086 0.006972 0.000444 0.000160 0.003401 0.009661 0.000543 0.001959 0.003931 0.008290 0.000510 0.001791
Lipid Particles 0.003362 0.002054 0.001016 0.000826 0.006544 0.002500 0.000754 0.000235 0.004080 0.002273 0.000841 0.000290
Mitochondria 0.002693 0.002342 0.000045 0.000034 0.002017 0.005041 0.000294 0.000735 0.002541 0.003665 0.000211 0.000670
Mitotic Spindle 0.000553 0.003794 0.002343 0.005074 0.001307 0.006858 0.000556 0.006899 0.000723 0.005296 0.001150 0.006728
None 0.002870 0.001324 0.000755 0.005226 0.001078 0.001108 0.001289 0.001200 0.002466 0.001218 0.001111 0.001577
Nuclear Periphery 0.000161 0.000095 0.000214 0.000193 0.000170 0.000166 0.000169 0.000046 0.000163 0.000130 0.000184 0.000059
Nuclear Periphery Foci 0.000334 0.000768 0.012497 0.003073 0.001132 0.000227 0.010115 0.000922 0.000514 0.000503 0.010907 0.001124
Nucleolus 0.000530 0.000449 0.000077 0.000120 0.000274 0.000580 0.000081 0.000082 0.000472 0.000513 0.000080 0.000085
Nucleus 0.045523 0.026809 0.041939 0.029179 0.025219 0.019215 0.029772 0.013020 0.040938 0.023085 0.033815 0.014533
Peroxisomes 0.001595 0.002502 0.000235 0.000553 0.001755 0.004747 0.000376 0.001491 0.001631 0.003603 0.000329 0.001404
Vacuole 0.079830 0.075750 0.079347 0.384591* 0.080294 0.108482 0.115960 0.079375 0.079935 0.091801 0.103794 0.107956
Vacuole Periphery 0.001764 0.002381 0.000217 0.000163 0.001469 0.003757 0.000206 0.000330 0.001697 0.003056 0.000209 0.000314

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.62 -4.74 -4.22 -0.96 3.01 -1.26 -1.16 -0.74 -0.16 0.38 -3.40 -2.50 -1.31 -0.48 0.64
Bud Neck 1.03 10.99 8.16 8.06 -1.88 -5.44 5.47 5.63 15.06 -0.22 -4.20 12.10 12.88 18.94 -0.22
Bud Site -8.80 -2.48 -2.70 4.43 0.37 -8.55 -1.47 -3.04 5.64 0.38 -12.53 -2.16 -4.39 5.78 0.28
Cell Periphery -3.13 -4.85 1.17 3.24 5.05 6.81 -0.05 9.85 5.68 1.66 0.98 -1.50 7.81 6.88 2.51
Cytoplasm -1.81 -4.48 7.20 7.75 8.54 1.85 -9.42 -18.88 -27.76 -2.81 3.12 -8.89 -17.56 -20.71 -1.06
Cytoplasmic Foci 1.55 17.56 23.31 21.22 1.56 4.91 25.75 28.83 35.95 0.85 1.36 31.85 41.73 44.20 2.00
Eisosomes 4.04 9.84 10.20 6.46 -0.87 5.80 12.87 15.59 19.24 4.61 4.50 15.96 24.17 23.63 5.30
Endoplasmic Reticulum -1.82 -2.39 0.37 1.86 2.44 0.76 0.69 5.58 2.70 3.24 -1.09 -1.95 9.09 4.41 4.54
Endosome -0.82 8.54 14.31 12.81 1.54 -2.36 11.83 13.43 22.25 3.30 -5.23 15.57 21.02 25.83 4.10
Golgi -4.25 12.40 13.63 11.28 6.81 -6.37 6.19 3.68 8.85 -1.57 -7.45 13.03 6.42 11.16 -1.51
Lipid Particles 3.24 4.95 5.56 3.55 0.43 2.64 3.74 4.08 12.20 4.87 3.94 6.84 8.36 14.49 5.99
Mitochondria 0.45 4.42 4.42 4.04 0.16 -2.25 1.78 1.53 5.23 -0.98 -1.52 4.57 3.93 6.03 -1.21
Mitotic Spindle -3.46 -1.57 -2.51 -0.69 -1.42 -3.42 0.56 -2.51 1.13 -3.46 -5.29 -1.19 -3.88 0.33 -3.24
None 4.47 6.88 0.50 -0.70 -1.19 -0.08 -0.19 0.06 0.16 0.29 4.45 5.33 3.62 0.20 -0.53
Nuclear Periphery 1.99 -2.54 -3.79 -4.86 -2.04 0.06 -0.68 1.78 1.22 4.68 0.90 -2.15 1.09 -0.11 4.32
Nuclear Periphery Foci -1.85 -6.91 -6.33 -4.88 5.33 7.32 -6.91 0.80 -12.02 7.02 0.05 -10.01 -9.00 -5.17 9.34
Nucleolus 0.48 10.95 6.73 2.19 -2.11 -2.23 6.85 6.54 3.55 -1.32 -0.49 12.02 11.39 4.40 -2.09
Nucleus 9.77 0.03 2.58 -1.42 1.73 3.73 -2.22 4.72 1.24 4.85 12.86 1.53 15.46 3.62 6.37
Peroxisomes -3.72 10.84 9.11 9.14 -2.78 -6.75 6.29 2.33 7.49 -1.77 -7.91 9.54 2.54 7.60 -1.96
Vacuole 1.01 -2.24 -14.41 -14.53 -10.58 -5.63 -5.30 -8.95 -4.18 1.37 -4.44 -5.77 -15.19 -12.68 -1.84
Vacuole Periphery -2.59 13.38 15.17 10.85 2.89 -5.54 5.95 5.69 10.02 -0.77 -6.02 15.06 14.19 13.80 -0.28
Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsm3

Hsm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available