Standard name
Human Ortholog
Description Protein that binds to the Rap1p C-terminus; acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; involved in control of DNA replication; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.05 – 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.14 0.43 0.29 0.23 0.22 0.2 0.19 0.17 – 0.13 0.11 0.35 0.43 0.46 0.35 0.28 0.36 0 0 0.1 0.1 0.12 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0
Endosome 0.48 0.26 0.11 0 0.22 0 0.05 0 0 – 0 0 0.2 0.27 0.11 0.36 0.45 0.3 0.34 0.33 0.27 0.25 0.26 0.3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.31 0.34 0.6 0.42 0.69 0.7 0.74 0.83 – 0.85 0.88 0.17 0.07 0.28 0.14 0.05 0.12 0.09 0.11 0.19 0.13 0.13 0.14
Nucleus 0.05 0 0 0 0 0 0.05 0.05 0 – 0.06 0.05 0.08 0.11 0.06 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.08 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.24 0.06 0.17 0.12 0.18 0.11 0.15 0.09 0.06 – 0.06 0.07 0.09 0.09 0 0.2 0.24 0.23 0.28 0.24 0.19 0.29 0.26 0.27
Vac/Vac Membrane 0.22 0.39 0 0 0.12 0 0 0 0 – 0 0 0.22 0.12 0.12 0.18 0.16 0.13 0 0.05 0.05 0.05 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 – 0 0 0 1 0 0 0 0 2 4 7 2 5 3
Bud 0 0 5 12 12 13 26 27 19 – 29 23 0 1 7 1 1 2 2 2 6 0 8 2
Bud Neck 1 0 8 0 4 4 4 4 1 – 0 1 0 1 0 0 0 2 0 0 1 0 2 1
Bud Site 1 0 1 2 4 4 11 17 7 – 6 16 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 23 29 138 88 76 92 99 124 68 – 44 50 78 103 132 43 46 54 5 12 47 24 46 29
Endoplasmic Reticulum 3 3 0 0 0 0 1 1 0 – 0 0 0 2 4 7 9 13 0 1 6 2 2 6
Endosome 95 55 34 13 72 14 23 16 4 – 3 2 45 66 30 44 75 46 97 99 131 62 104 98
Golgi 1 2 0 0 0 0 0 0 0 – 0 0 0 0 0 1 2 1 5 2 1 2 0 1
Mitochondria 15 67 111 180 138 284 344 471 340 – 284 401 39 16 81 17 8 18 25 32 94 33 52 46
Nucleus 10 4 4 4 7 9 24 29 16 – 19 21 19 27 17 5 4 2 6 5 4 4 5 0
Nuclear Periphery 15 7 0 2 0 0 2 5 2 – 1 2 4 2 2 0 1 3 26 24 19 7 9 6
Nucleolus 8 3 1 2 3 2 5 10 9 – 5 8 7 0 0 2 1 4 7 9 9 5 6 4
Peroxisomes 1 0 2 4 16 3 10 4 1 – 0 2 0 1 0 2 1 3 2 1 7 3 2 5
SpindlePole 47 13 55 35 60 45 74 59 26 – 21 30 21 21 12 25 40 34 79 71 94 72 101 89
Vac/Vac Membrane 44 83 7 9 40 15 16 23 8 – 13 16 50 29 33 22 27 19 11 16 24 11 24 14
Unique Cell Count 199 213 323 300 329 410 489 637 409 334 456 224 242 285 123 165 151 287 302 492 252 396 327
Labelled Cell Count 264 266 367 351 432 486 639 790 501 425 572 263 270 318 169 215 202 287 302 492 252 396 327


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.1 3.7 3.4 3.8 3.4 3.1 3.2 2.8 – 3.0 2.7 4.3 4.3 3.7 5.7 6.3 5.8 4.3 4.3 4.7
Std Deviation (1e-4) 0.7 0.9 1.6 1.3 1.4 1.6 0.9 1.3 1.2 – 1.4 0.9 1.1 1.1 1.1 1.7 2.5 2.0 1.3 1.2 1.4
Intensity Change (Log2) – – – -0.11 0.03 -0.14 -0.26 -0.2 -0.4 – -0.32 -0.47 0.21 0.21 0.02 0.62 0.77 0.65 0.23 0.21 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.0 5.8 4.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2515 3.5162 3.4635 2.8496 3.8622 3.5664 2.5303 2.4385 2.3306 2.8387 1.7211 2.0744 3.5495 3.5869 3.192 3.4163 3.7652 3.3274
Actin 0.0275 0.0028 0.0172 0.0195 0.0185 0.0042 0.0486 0.0028 0.0151 0.0359 0.0014 0.004 0.0183 0.0014 0.0096 0.0048 0.0025 0.0019
Bud 0.0008 0.0037 0.0003 0.0002 0.0004 0.0001 0.0003 0.0002 0.0001 0.0019 0 0.0048 0.0004 0.0001 0.0001 0 0.0001 0.0001
Bud Neck 0.0029 0.0019 0.0018 0.0021 0.0115 0.0105 0.0007 0.0012 0.0009 0.0135 0.001 0.0042 0.0014 0.0006 0.0008 0.0003 0.0023 0.006
Bud Periphery 0.0018 0.0007 0.0007 0.0003 0.0008 0.0004 0.0005 0.0002 0.0002 0.0027 0.0001 0.0025 0.0005 0.0001 0.0003 0.0001 0.0002 0.0003
Bud Site 0.0033 0.0047 0.004 0.0007 0.0016 0.0006 0.001 0.0016 0.0012 0.0193 0.0002 0.0005 0.0038 0.0005 0.0009 0.0001 0.001 0.0003
Cell Periphery 0.0008 0.0003 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.001 0 0.0002 0.0001 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.0425 0.0417 0.0223 0.0023 0.049 0.0241 0.0503 0.0307 0.0113 0.0032 0.0286 0.0133 0.0124 0.0181 0.0134 0.0102 0.0051 0.0134
Cytoplasmic Foci 0.0343 0.0158 0.0213 0.0206 0.0275 0.0104 0.0146 0.0113 0.0228 0.052 0.0226 0.0068 0.0179 0.0068 0.0141 0.0165 0.0442 0.0079
Eisosomes 0.0008 0.0001 0.0003 0.0002 0.0004 0.0001 0.0003 0.0001 0.0001 0.001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.0179 0.0105 0.0057 0.0024 0.01 0.0075 0.0081 0.005 0.0026 0.0015 0.001 0.0015 0.0057 0.0058 0.0019 0.0022 0.0017 0.0034
Endosome 0.0388 0.0281 0.0183 0.0124 0.0362 0.0156 0.0337 0.0124 0.0083 0.0505 0.0135 0.0084 0.0327 0.0071 0.0119 0.0066 0.0166 0.0153
Golgi 0.0101 0.0027 0.0045 0.0056 0.0215 0.0052 0.0116 0.0015 0.003 0.0371 0.004 0.0058 0.0138 0.0019 0.0031 0.0027 0.0054 0.0026
Lipid Particles 0.0476 0.0521 0.0377 0.0846 0.1062 0.0333 0.0185 0.0126 0.0388 0.0625 0.1312 0.0182 0.0467 0.0385 0.0396 0.04 0.1336 0.0206
Mitochondria 0.0412 0.0015 0.0026 0.0009 0.0223 0.0076 0.0032 0.0008 0.0009 0.0628 0.0003 0.0041 0.0059 0.0007 0.0027 0.0006 0.0009 0.0008
None 0.0635 0.0338 0.0215 0.0033 0.0694 0.023 0.0471 0.0218 0.029 0.0034 0.0041 0.0259 0.017 0.0396 0.0147 0.0466 0.0524 0.0287
Nuclear Periphery 0.2667 0.2366 0.2783 0.2516 0.3057 0.1976 0.2087 0.2224 0.2264 0.1123 0.2001 0.2478 0.3824 0.3358 0.363 0.3142 0.1847 0.2722
Nucleolus 0.0264 0.0306 0.0375 0.0136 0.0066 0.0601 0.0111 0.0244 0.0205 0.0075 0.0447 0.0378 0.0148 0.0162 0.0304 0.0078 0.0489 0.0619
Nucleus 0.1119 0.2087 0.1019 0.0686 0.0743 0.2942 0.218 0.2682 0.2203 0.1266 0.1647 0.2508 0.1314 0.2832 0.1091 0.0348 0.0441 0.2452
Peroxisomes 0.0163 0.0023 0.0048 0.0122 0.0181 0.0016 0.0031 0.0014 0.0043 0.0319 0.0059 0.003 0.0088 0.0015 0.0039 0.0065 0.0215 0.0016
Punctate Nuclear 0.2278 0.3116 0.4123 0.4953 0.2086 0.2976 0.3163 0.3769 0.3898 0.3672 0.3734 0.3573 0.2798 0.2367 0.3757 0.5019 0.4308 0.3144
Vacuole 0.009 0.0067 0.0027 0.0008 0.0042 0.0034 0.0015 0.0022 0.0016 0.004 0.0007 0.0013 0.0023 0.0015 0.0013 0.0008 0.0009 0.0014
Vacuole Periphery 0.0083 0.003 0.004 0.0029 0.0069 0.0027 0.0027 0.0025 0.003 0.0022 0.0023 0.0018 0.0035 0.0037 0.0032 0.0031 0.0027 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.1079 10.8054 8.2726 14.1345 19.5276 23.9696 13.4399 10.0074 12.311 26.8984
Translational Efficiency 0.6609 0.6647 0.5357 0.3721 0.5536 0.7099 0.4789 0.4852 0.3728 0.4561

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1667 132 151 1386 1653 1377 1374 926 3320 1509 1525 2312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 534.94 742.43 969.51 799.52 599.50 701.72 806.03 774.14 567.08 705.28 822.22 789.35
Standard Deviation 57.63 81.34 102.99 125.89 75.27 94.00 97.39 101.67 74.37 93.67 109.46 117.45
Intensity Change Log 2 — 0.472878 0.857879 0.579757 — 0.227136 0.427074 0.368835 — 0.348240 0.646278 0.472144

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000199 0.000744 0.001191 0.001007 0.000131 0.000283 0.000567 0.000894 0.000165 0.000323 0.000628 0.000962
Bud Neck 0.012582 0.004390 0.002069 0.011403 0.006419 0.005231 0.004688 0.008608 0.009514 0.005158 0.004429 0.010284
Bud Site 0.002562 0.001976 0.019440 0.037646 0.001127 0.004271 0.006042 0.024094 0.001848 0.004071 0.007369 0.032218
Cell Periphery 0.000262 0.000130 0.000352 0.000651 0.000124 0.000105 0.000174 0.000175 0.000193 0.000107 0.000192 0.000460
Cytoplasm 0.327846* 0.062165 0.123958* 0.313981* 0.256769* 0.117019* 0.175825* 0.136900* 0.292457* 0.112221* 0.170689* 0.243057*
Cytoplasmic Foci 0.248700* 0.136110 0.020139 0.029556 0.155521 0.134741 0.015073 0.012267 0.202307* 0.134861 0.015575 0.022632
Eisosomes 0.000306 0.000136 0.000184 0.000068 0.000105 0.000092 0.000076 0.000058 0.000206 0.000096 0.000087 0.000064
Endoplasmic Reticulum 0.002934 0.004941 0.014978 0.003904 0.003193 0.003965 0.005067 0.007640 0.003063 0.004050 0.006048 0.005400
Endosome 0.007104 0.020211 0.007711 0.015466 0.012852 0.018916 0.005103 0.009062 0.009966 0.019030 0.005361 0.012901
Golgi 0.003161 0.007161 0.000249 0.004981 0.003814 0.008871 0.000343 0.002991 0.003486 0.008721 0.000334 0.004184
Lipid Particles 0.111821* 0.210938* 0.012648 0.006167 0.131564* 0.254115* 0.007194 0.008409 0.121651* 0.250338* 0.007734 0.007065
Mitochondria 0.010408 0.020638 0.000817 0.006752 0.014687 0.026065 0.002053 0.007942 0.012538 0.025590 0.001931 0.007228
Mitotic Spindle 0.002893 0.208444* 0.004539 0.156710* 0.044141 0.129666* 0.044915 0.310038* 0.023430 0.136557* 0.040917 0.218120*
None 0.027228 0.009866 0.004534 0.021982 0.034412 0.011674 0.023142 0.008693 0.030805 0.011516 0.021300 0.016659
Nuclear Periphery 0.004263 0.014717 0.004332 0.008882 0.016579 0.022832 0.015819 0.020223 0.010395 0.022122 0.014681 0.013424
Nuclear Periphery Foci 0.013213 0.099437 0.050616 0.019688 0.079217 0.097249 0.044765 0.157877 0.046076 0.097440 0.045344 0.075035
Nucleolus 0.009721 0.032304 0.008979 0.011233 0.037350 0.034897 0.030634 0.018330 0.023477 0.034670 0.028490 0.014075
Nucleus 0.196437* 0.129331 0.642092* 0.271964* 0.187621* 0.104404 0.599331* 0.233334* 0.192048* 0.106585 0.603565* 0.256492*
Peroxisomes 0.010232 0.025522 0.001397 0.004062 0.006594 0.015160 0.001652 0.001666 0.008421 0.016067 0.001627 0.003102
Vacuole 0.007615 0.008505 0.079298 0.065215 0.006777 0.008450 0.017221 0.028402 0.007198 0.008455 0.023368 0.050471
Vacuole Periphery 0.000513 0.002334 0.000475 0.008683 0.001003 0.001992 0.000314 0.002400 0.000757 0.002022 0.000330 0.006166

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.94 -7.78 -5.33 -0.57 4.63 -4.48 -13.42 -13.30 -11.46 -2.82 -2.81 -11.61 -10.11 -9.64 -0.15
Bud Neck 6.16 7.47 -0.30 -6.68 -8.25 0.84 -3.36 -5.58 -6.63 -3.07 4.82 0.55 -4.11 -8.74 -4.82
Bud Site 0.05 -1.75 -8.23 -8.22 -0.56 -2.55 -5.30 -5.74 -4.35 -3.77 -1.87 -4.30 -10.16 -8.04 -6.19
Cell Periphery 4.84 -3.48 -0.32 -1.53 1.10 2.08 -4.99 -3.34 -4.62 0.27 7.21 -0.25 -0.86 -2.32 -0.81
Cytoplasm 20.33 15.94 8.78 -13.40 -9.88 14.17 13.18 12.23 -0.68 0.54 25.00 23.78 12.49 -11.66 -10.22
Cytoplasmic Foci 7.44 37.15 40.50 7.39 -1.47 3.55 29.86 30.70 26.04 1.88 12.33 48.87 49.19 26.29 -0.91
Eisosomes 6.65 0.72 9.99 2.23 7.66 2.51 -3.25 2.62 0.66 5.34 10.31 5.15 9.99 -0.58 6.72
Endoplasmic Reticulum -2.55 -11.19 -10.93 -5.32 8.01 -2.15 -14.13 -14.37 -12.93 -5.64 -2.57 -16.32 -18.13 -15.64 -0.68
Endosome -4.86 -3.66 -3.86 3.52 1.31 -4.48 5.94 3.63 7.19 -1.41 -8.37 2.25 -0.00 7.83 -1.88
Golgi -1.98 10.83 3.71 2.98 -3.04 -4.11 8.43 3.65 6.08 -1.39 -4.77 13.11 5.21 6.82 -3.14
Lipid Particles -2.74 20.21 23.63 8.01 4.90 -11.99 25.44 25.27 30.76 -0.13 -13.66 33.75 34.70 32.07 3.00
Mitochondria -2.83 11.52 7.30 5.38 -7.05 -5.14 11.98 6.42 9.36 -1.48 -6.59 15.15 10.10 11.52 -2.67
Mitotic Spindle -7.31 -1.60 -17.28 3.04 -14.50 -10.18 -3.57 -17.63 -10.38 -15.08 -15.93 -7.94 -22.86 -4.77 -13.94
None 5.30 11.59 6.88 -0.18 -5.66 9.46 8.19 12.12 3.24 4.63 10.34 9.30 12.82 2.07 2.95
Nuclear Periphery -2.99 -2.25 -11.16 -1.25 -9.49 -3.36 -1.86 -4.24 -1.16 -2.68 -7.92 -6.42 -9.09 0.84 -1.54
Nuclear Periphery Foci -5.54 -3.86 -3.26 4.88 3.07 -1.63 4.51 -4.42 -3.08 -7.78 -8.95 -2.15 -3.30 5.76 -0.83
Nucleolus -4.26 -0.74 -1.64 3.85 0.20 1.86 -2.25 6.62 4.42 7.92 -4.72 -7.66 5.89 8.65 11.17
Nucleus 2.64 -14.61 -18.08 -10.80 7.89 9.03 -34.46 -8.69 -14.72 19.72 11.56 -38.54 -19.64 -26.94 20.40
Peroxisomes -3.47 11.57 11.00 5.50 -0.15 -5.74 11.22 12.69 11.08 2.28 -5.65 15.06 15.61 12.09 1.71
Vacuole -0.30 -9.32 -22.88 -22.22 0.59 -1.69 -11.16 -12.10 -11.64 -5.59 -1.01 -13.56 -25.50 -24.89 -12.78
Vacuole Periphery -3.73 -1.36 -3.32 -1.13 -3.19 -2.84 4.27 -0.43 1.49 -1.82 -3.97 4.72 -3.18 -0.74 -3.91
Standard name
Human Ortholog
Description Protein that binds to the Rap1p C-terminus; acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; involved in control of DNA replication; contributes to resection of DNA double strand breaks (DSBs); deletion results in telomere elongation
Localization
Cell Percentages nucleus (10%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rif1

Rif1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rif1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available