Standard name | |
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Human Ortholog | |
Description | Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 4 | 4 | 3 | 10 | 0 | 0 | 11 | 0 | 2 | 0 | 0 | 1 | 2 | 0 | 3 | 4 |
Bud | 0 | 2 | 9 | 0 | 2 | 1 | 6 | 10 | 2 | 9 | 8 | 1 | 6 | 3 | 35 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 7 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 5 | 3 | 5 | 13 | 15 | 28 | 42 | 23 | 40 | 20 | 34 | 11 | 31 | 12 | 13 | 10 | 9 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 103 | 258 | 262 | 276 | 447 | 480 | 515 | 494 | 315 | 366 | 219 | 263 | 499 | 758 | 450 | 302 | 170 | 132 | 99 | 273 | 244 | 186 | 424 | 453 |
Endoplasmic Reticulum | 0 | 1 | 1 | 2 | 1 | 9 | 24 | 21 | 23 | 21 | 23 | 19 | 1 | 1 | 3 | 9 | 6 | 5 | 0 | 1 | 6 | 4 | 9 | 6 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 3 | 0 | 1 | 0 | 2 | 0 | 1 | 210 | 11 | 9 | 0 | 0 | 1 | 0 | 0 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 3 | 1 |
Mitochondria | 5 | 11 | 9 | 12 | 30 | 93 | 139 | 177 | 163 | 248 | 169 | 224 | 1 | 0 | 0 | 19 | 8 | 6 | 0 | 3 | 4 | 0 | 6 | 3 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 3 | 1 | 3 | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 23 | 6 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 105 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 119 | 9 | 6 | 2 | 0 | 1 | 1 | 0 | 1 | 2 |
Vac/Vac Membrane | 0 | 2 | 0 | 0 | 7 | 2 | 26 | 61 | 20 | 53 | 24 | 39 | 1 | 1 | 15 | 13 | 20 | 8 | 0 | 4 | 6 | 2 | 3 | 7 |
Unique Cell Count | 104 | 275 | 270 | 284 | 474 | 524 | 652 | 692 | 459 | 600 | 381 | 481 | 502 | 761 | 793 | 333 | 207 | 145 | 104 | 291 | 275 | 202 | 471 | 497 |
Labelled Cell Count | 109 | 286 | 286 | 296 | 506 | 603 | 748 | 818 | 558 | 754 | 473 | 596 | 519 | 796 | 984 | 384 | 241 | 168 | 104 | 291 | 275 | 202 | 471 | 497 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.8 | 6.6 | 6.1 | 6.9 | 7.3 | 7.4 | 7.9 | 8.0 | 8.1 | 8.2 | 8.6 | 7.9 | 9.4 | 10.9 | 9.7 | 11.5 | 12.0 | 6.3 | 6.3 | 7.0 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.9 | 1.4 | 1.7 | 1.6 | 1.7 | 2.1 | 2.5 | 2.5 | 2.8 | 3.2 | 1.6 | 2.2 | 2.4 | 2.4 | 3.3 | 3.0 | 1.8 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.06 | 0.14 | 0.17 | 0.26 | 0.27 | 0.29 | 0.31 | 0.38 | 0.27 | 0.51 | 0.72 | 0.56 | 0.8 | 0.86 | -0.08 | -0.07 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | -1.9 | 0 | -1.8 | -1.0 | 0 | -2.1 | 0 | 0.8 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.5 | 1.6 | 2.4 | 3.3 | 2.7 | 3.5 | 2.8 | 3.6 | 0 | 2.3 | 0 | 2.1 | 2.5 | 0 |
Cytoplasm | 0.1 | -1.7 | -2.9 | -6.9 | -8.7 | -9.1 | -11.0 | -11.3 | -12.1 | 2.6 | 3.5 | -12.2 | -3.2 | -5.5 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.8 | 2.5 | 3.4 | 2.7 | 3.8 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.5 | 1.8 | 5.8 | 6.8 | 7.8 | 9.9 | 11.4 | 11.6 | 12.3 | 0 | 0 | 0 | 1.4 | 0.3 | 0.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.8 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.3 | 5.0 | 3.5 | 5.0 | 4.2 | 4.8 | 0 | 0 | 0 | 3.3 | 5.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7836 | 4.5296 | 4.3662 | 3.3387 | 3.7227 | 3.9183 | 3.6115 | 5.2413 | 4.8328 | 3.9303 | 4.3774 | 4.729 | 6.5935 | 7.8837 | 7.9661 | 8.2862 | 5.672 | 8.2679 |
Actin | 0.0072 | 0.0008 | 0.0125 | 0.0037 | 0.0003 | 0.0031 | 0.0463 | 0.014 | 0.0108 | 0.0142 | 0.0199 | 0.002 | 0.1062 | 0.0019 | 0.0364 | 0.0053 | 0.0382 | 0.0005 |
Bud | 0.0003 | 0.0007 | 0.0017 | 0.0021 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0005 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0008 | 0.0013 | 0.0007 | 0.0001 |
Bud Neck | 0.0019 | 0.0004 | 0.0038 | 0.0011 | 0.0003 | 0.0026 | 0.0009 | 0.0004 | 0.0008 | 0.0245 | 0.0007 | 0.0006 | 0.0019 | 0.0002 | 0.0004 | 0.0005 | 0.0008 | 0.0005 |
Bud Periphery | 0.0003 | 0.0003 | 0.0008 | 0.0011 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0007 | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0 |
Bud Site | 0.0012 | 0.0038 | 0.0047 | 0.0008 | 0.0001 | 0.0004 | 0.0014 | 0.0037 | 0.009 | 0.0047 | 0.0027 | 0.0001 | 0.0018 | 0.0005 | 0.0045 | 0.0003 | 0.0007 | 0.0001 |
Cell Periphery | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.8013 | 0.938 | 0.8745 | 0.8854 | 0.9583 | 0.8962 | 0.7054 | 0.9339 | 0.8197 | 0.6455 | 0.8449 | 0.9394 | 0.8437 | 0.951 | 0.9143 | 0.9118 | 0.7881 | 0.9572 |
Cytoplasmic Foci | 0.0306 | 0.0079 | 0.0237 | 0.0184 | 0.0073 | 0.0316 | 0.0593 | 0.0036 | 0.0329 | 0.0589 | 0.0192 | 0.0262 | 0.0031 | 0.0025 | 0.0103 | 0.0216 | 0.0595 | 0.0278 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0013 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.002 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0159 | 0.0035 | 0.0108 | 0.0044 | 0.0034 | 0.0042 | 0.0148 | 0.0043 | 0.0068 | 0.0035 | 0.0068 | 0.0041 | 0.0048 | 0.0336 | 0.004 | 0.0032 | 0.0055 | 0.0053 |
Endosome | 0.033 | 0.0046 | 0.0228 | 0.0271 | 0.0084 | 0.0141 | 0.0373 | 0.0034 | 0.0166 | 0.0708 | 0.0204 | 0.0093 | 0.0029 | 0.0024 | 0.0094 | 0.0176 | 0.0445 | 0.0029 |
Golgi | 0.0047 | 0.0015 | 0.0037 | 0.0045 | 0.0003 | 0.0047 | 0.0086 | 0.0005 | 0.0127 | 0.059 | 0.0101 | 0.0071 | 0.0042 | 0.0005 | 0.0049 | 0.0022 | 0.0112 | 0.0003 |
Lipid Particles | 0.0028 | 0.0003 | 0.0033 | 0.0029 | 0 | 0.0027 | 0.0216 | 0.0003 | 0.0058 | 0.0181 | 0.0025 | 0.0001 | 0.0031 | 0.0001 | 0.001 | 0.001 | 0.0238 | 0.0001 |
Mitochondria | 0.0165 | 0.0002 | 0.0105 | 0.0216 | 0.0001 | 0.001 | 0.0137 | 0.0022 | 0.002 | 0.0457 | 0.0354 | 0.0011 | 0.001 | 0.0002 | 0.0046 | 0.0009 | 0.0007 | 0.0001 |
None | 0.0653 | 0.0318 | 0.0142 | 0.0093 | 0.0048 | 0.0278 | 0.0685 | 0.0295 | 0.0538 | 0.0031 | 0.0234 | 0.0058 | 0.0203 | 0.0027 | 0.003 | 0.022 | 0.0052 | 0.0009 |
Nuclear Periphery | 0.004 | 0.0006 | 0.0013 | 0.0021 | 0.0012 | 0.0012 | 0.0033 | 0.0003 | 0.0009 | 0.0013 | 0.0021 | 0.0005 | 0.0007 | 0.0016 | 0.0006 | 0.0007 | 0.0019 | 0.0004 |
Nucleolus | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0016 | 0.0015 | 0.0016 | 0.0025 | 0.0015 | 0.0021 | 0.0011 | 0.0006 | 0.0009 | 0.0015 | 0.001 | 0.0007 | 0.0006 | 0.0009 | 0.0007 | 0.0011 | 0.0011 | 0.0007 |
Peroxisomes | 0.0035 | 0.0002 | 0.0034 | 0.0032 | 0 | 0.0013 | 0.0079 | 0.0004 | 0.0121 | 0.0355 | 0.0043 | 0.0011 | 0.0014 | 0 | 0.0026 | 0.0006 | 0.0078 | 0.0022 |
Punctate Nuclear | 0.0014 | 0.0002 | 0.0005 | 0.0005 | 0.0001 | 0.0013 | 0.0049 | 0.0002 | 0.0111 | 0.0046 | 0.0019 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.006 | 0.0002 |
Vacuole | 0.0054 | 0.0029 | 0.0047 | 0.0065 | 0.0118 | 0.0038 | 0.0019 | 0.0011 | 0.0023 | 0.0048 | 0.0024 | 0.0005 | 0.0006 | 0.0012 | 0.001 | 0.0062 | 0.0029 | 0.0006 |
Vacuole Periphery | 0.002 | 0.0003 | 0.0008 | 0.0017 | 0.0018 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0015 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0027 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 143.8904 | 74.1587 | 130.5111 | 255.3138 | 144.2034 | 289.8227 | 177.8329 | 256.6211 | 398.5894 | 197.337 |
Translational Efficiency | 1.1852 | 1.0354 | 1.2719 | 1.8051 | 1.3805 | 1.2924 | 1.3356 | 1.7289 | 1.4194 | 1.3632 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1871 | 831 | 367 | 1389 | 1723 | 392 | 2173 | 2735 | 3594 | 1223 | 2540 | 4124 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 751.09 | 811.19 | 1327.22 | 1050.89 | 791.45 | 821.49 | 935.60 | 1169.96 | 770.44 | 814.49 | 992.18 | 1129.86 |
Standard Deviation | 103.83 | 121.63 | 192.99 | 238.66 | 109.85 | 131.94 | 158.10 | 219.63 | 108.65 | 125.12 | 213.83 | 233.11 |
Intensity Change Log 2 | — | 0.111054 | 0.821350 | 0.484554 | — | 0.053745 | 0.241394 | 0.563889 | — | 0.081934 | 0.552814 | 0.525804 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000399 | 0.000324 | 0.000411 | 0.000982 | 0.000115 | 0.000525 | 0.000095 | 0.000100 | 0.000263 | 0.000388 | 0.000141 | 0.000397 |
Bud Neck | 0.021050 | 0.012559 | 0.002338 | 0.003415 | 0.008284 | 0.024504 | 0.001453 | 0.001734 | 0.014930 | 0.016387 | 0.001581 | 0.002300 |
Bud Site | 0.030561 | 0.032303 | 0.013132 | 0.045715 | 0.010024 | 0.029922 | 0.010853 | 0.009084 | 0.020715 | 0.031540 | 0.011182 | 0.021422 |
Cell Periphery | 0.000151 | 0.000140 | 0.000189 | 0.000175 | 0.000152 | 0.000142 | 0.000061 | 0.000066 | 0.000151 | 0.000141 | 0.000080 | 0.000103 |
Cytoplasm | 0.584129* | 0.562038* | 0.660732* | 0.715058* | 0.601019* | 0.399033* | 0.820324* | 0.845081* | 0.592226* | 0.509791* | 0.797265* | 0.801288* |
Cytoplasmic Foci | 0.196834* | 0.225304* | 0.070959 | 0.037432 | 0.205681* | 0.258568* | 0.038984 | 0.016431 | 0.201075* | 0.235966* | 0.043604 | 0.023504 |
Eisosomes | 0.000092 | 0.000075 | 0.000014 | 0.000015 | 0.000093 | 0.000127 | 0.000008 | 0.000010 | 0.000093 | 0.000091 | 0.000009 | 0.000011 |
Endoplasmic Reticulum | 0.000915 | 0.001277 | 0.000915 | 0.000493 | 0.001970 | 0.001817 | 0.000240 | 0.000086 | 0.001421 | 0.001450 | 0.000337 | 0.000223 |
Endosome | 0.026445 | 0.030970 | 0.008367 | 0.008064 | 0.020717 | 0.056710 | 0.002239 | 0.001032 | 0.023699 | 0.039220 | 0.003124 | 0.003401 |
Golgi | 0.004357 | 0.007813 | 0.000771 | 0.006277 | 0.004818 | 0.007941 | 0.000207 | 0.000212 | 0.004578 | 0.007854 | 0.000288 | 0.002255 |
Lipid Particles | 0.003726 | 0.002468 | 0.002279 | 0.000987 | 0.004277 | 0.005545 | 0.000337 | 0.000193 | 0.003990 | 0.003454 | 0.000617 | 0.000461 |
Mitochondria | 0.001648 | 0.003161 | 0.000018 | 0.003483 | 0.001547 | 0.004628 | 0.000134 | 0.000979 | 0.001600 | 0.003631 | 0.000117 | 0.001822 |
Mitotic Spindle | 0.004091 | 0.001975 | 0.001090 | 0.037319 | 0.000367 | 0.007084 | 0.002854 | 0.005233 | 0.002306 | 0.003612 | 0.002599 | 0.016040 |
None | 0.004656 | 0.001067 | 0.001036 | 0.003306 | 0.001918 | 0.000906 | 0.001444 | 0.003310 | 0.003344 | 0.001015 | 0.001385 | 0.003308 |
Nuclear Periphery | 0.000152 | 0.000124 | 0.000165 | 0.000297 | 0.000148 | 0.000317 | 0.000089 | 0.000080 | 0.000150 | 0.000186 | 0.000100 | 0.000153 |
Nuclear Periphery Foci | 0.000633 | 0.000683 | 0.008661 | 0.001941 | 0.000439 | 0.000642 | 0.000916 | 0.000230 | 0.000540 | 0.000670 | 0.002035 | 0.000806 |
Nucleolus | 0.000893 | 0.000335 | 0.000109 | 0.000313 | 0.000379 | 0.001222 | 0.000120 | 0.000223 | 0.000646 | 0.000619 | 0.000119 | 0.000254 |
Nucleus | 0.029486 | 0.015270 | 0.021367 | 0.039714 | 0.033036 | 0.025245 | 0.026929 | 0.039703 | 0.031188 | 0.018467 | 0.026125 | 0.039706 |
Peroxisomes | 0.007377 | 0.004743 | 0.000386 | 0.002080 | 0.001619 | 0.009871 | 0.000600 | 0.000515 | 0.004616 | 0.006387 | 0.000569 | 0.001042 |
Vacuole | 0.080634 | 0.093097 | 0.206584* | 0.091569 | 0.100402 | 0.161371 | 0.091938 | 0.075453 | 0.090111 | 0.114980 | 0.108503 | 0.080881 |
Vacuole Periphery | 0.001770 | 0.004275 | 0.000477 | 0.001365 | 0.002995 | 0.003881 | 0.000177 | 0.000245 | 0.002358 | 0.004148 | 0.000220 | 0.000622 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.58 | -0.32 | -0.89 | -2.18 | -0.91 | -2.01 | 1.09 | 1.27 | 2.13 | 0.61 | -1.35 | 1.72 | 0.33 | 1.98 | -2.45 |
Bud Neck | 4.12 | 11.71 | 11.77 | 7.33 | 0.28 | -6.57 | 11.83 | 11.28 | 9.49 | -1.90 | -0.92 | 15.31 | 14.84 | 11.82 | -2.44 |
Bud Site | -0.53 | 7.39 | 2.11 | 2.41 | -3.89 | -6.34 | 0.93 | 2.71 | 7.13 | 1.35 | -4.88 | 8.13 | 5.39 | 8.65 | -2.58 |
Cell Periphery | 0.92 | -2.33 | 1.78 | 0.99 | 3.30 | 0.62 | 9.45 | 9.13 | 4.70 | 0.81 | 1.18 | 8.28 | 8.41 | 5.78 | 0.40 |
Cytoplasm | 1.83 | -3.77 | -8.65 | -8.78 | -1.37 | 13.34 | -24.06 | -27.34 | -28.28 | -2.63 | 8.69 | -26.92 | -28.13 | -27.47 | 1.49 |
Cytoplasmic Foci | -3.31 | 20.23 | 33.31 | 28.14 | 9.77 | -4.86 | 35.86 | 42.07 | 24.34 | 9.40 | -5.30 | 44.21 | 53.87 | 38.14 | 10.65 |
Eisosomes | 2.67 | 16.78 | 17.20 | 12.49 | 0.14 | -3.00 | 20.14 | 19.88 | 11.70 | 0.90 | 0.28 | 26.91 | 26.25 | 17.36 | -0.58 |
Endoplasmic Reticulum | -1.27 | 0.44 | 4.01 | 3.59 | 4.03 | 0.22 | 5.86 | 6.44 | 2.22 | 3.77 | -0.06 | 6.81 | 7.93 | 4.22 | 3.59 |
Endosome | -1.61 | 11.23 | 14.19 | 11.55 | 4.96 | -7.55 | 20.19 | 21.48 | 12.02 | 5.78 | -6.48 | 22.78 | 23.70 | 17.05 | 3.96 |
Golgi | -3.33 | 11.17 | 2.66 | 4.54 | -1.98 | -1.83 | 17.52 | 17.49 | 4.75 | 0.47 | -3.70 | 20.71 | 11.43 | 7.91 | -2.36 |
Lipid Particles | 2.69 | 3.04 | 7.26 | 4.28 | 5.41 | -0.95 | 11.97 | 12.30 | 4.26 | 2.60 | 1.12 | 13.53 | 14.14 | 6.45 | 2.32 |
Mitochondria | -1.32 | 4.54 | 0.48 | 1.47 | -1.81 | -1.56 | 5.28 | 2.36 | 2.08 | -1.18 | -2.05 | 6.53 | 2.07 | 2.81 | -2.05 |
Mitotic Spindle | 1.59 | 3.06 | -5.65 | -6.34 | -7.01 | -2.14 | -4.58 | -6.46 | 0.37 | -2.96 | -0.96 | -0.49 | -7.13 | -4.41 | -6.89 |
None | 4.56 | 4.50 | 2.64 | -3.99 | -3.61 | 2.06 | 1.78 | -1.63 | -3.64 | -4.37 | 5.02 | 4.74 | 1.69 | -5.56 | -5.60 |
Nuclear Periphery | 0.59 | -1.31 | -4.43 | -3.82 | -3.82 | -1.76 | 0.75 | -0.05 | 1.78 | -1.55 | -0.78 | 0.51 | -3.26 | -0.63 | -4.98 |
Nuclear Periphery Foci | 0.26 | -9.07 | -4.55 | -6.12 | 8.03 | -1.80 | -6.30 | 2.56 | 2.64 | 8.01 | -0.73 | -8.52 | -2.21 | -1.63 | 7.88 |
Nucleolus | 3.99 | 5.59 | 4.43 | 1.52 | -2.39 | -1.96 | 8.87 | 2.25 | 2.25 | -2.31 | 0.35 | 6.87 | 4.86 | 2.57 | -3.03 |
Nucleus | 8.95 | 3.03 | -10.24 | -15.58 | -10.43 | 2.32 | -0.34 | -5.10 | -5.04 | -5.18 | 8.33 | -0.60 | -11.03 | -16.61 | -9.86 |
Peroxisomes | 3.28 | 10.55 | 9.34 | 7.06 | -2.78 | -4.68 | 5.57 | 7.36 | 5.36 | 0.12 | -2.24 | 10.94 | 10.95 | 8.67 | -0.92 |
Vacuole | -2.76 | -9.55 | -15.37 | -11.81 | 2.30 | -6.25 | -3.97 | -2.68 | 5.01 | 1.45 | -5.49 | -10.12 | -12.42 | -3.71 | -0.40 |
Vacuole Periphery | -3.19 | 6.19 | 4.89 | 4.87 | -0.65 | -1.33 | 13.38 | 13.12 | 6.28 | -0.33 | -3.12 | 14.45 | 13.02 | 7.00 | -1.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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