Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; computational analysis suggests that Gid7p and Moh1p have similar functions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 3 |
Bud | 0 | 1 | 1 | 3 | 1 | 1 | 4 | 5 | 9 | – | 2 | 6 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 7 | 12 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 3 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 106 | 58 | 151 | 116 | 144 | 140 | 232 | 210 | 123 | – | 105 | 152 | 164 | 180 | 237 | 151 | 78 | 104 | 126 | 69 | 146 | 71 | 146 | 137 |
Endoplasmic Reticulum | 5 | 1 | 3 | 1 | 5 | 0 | 2 | 11 | 3 | – | 1 | 7 | 4 | 3 | 1 | 11 | 1 | 7 | 3 | 0 | 2 | 6 | 17 | 18 |
Endosome | 8 | 0 | 1 | 2 | 4 | 4 | 8 | 4 | 2 | – | 0 | 4 | 11 | 30 | 67 | 6 | 4 | 5 | 7 | 4 | 3 | 3 | 9 | 12 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 17 | 11 | 1 | 11 | 11 | 52 | 75 | 41 | 144 | – | 162 | 91 | 17 | 4 | 5 | 2 | 2 | 1 | 10 | 3 | 25 | 13 | 40 | 28 |
Nucleus | 2 | 2 | 6 | 6 | 13 | 21 | 47 | 54 | 22 | – | 19 | 43 | 4 | 7 | 11 | 16 | 9 | 11 | 23 | 26 | 19 | 8 | 22 | 19 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 9 | 5 | 7 | 4 | 6 | 12 |
Vac/Vac Membrane | 63 | 37 | 34 | 21 | 60 | 48 | 104 | 94 | 75 | – | 44 | 64 | 138 | 199 | 224 | 132 | 89 | 101 | 29 | 36 | 43 | 39 | 111 | 108 |
Unique Cell Count | 176 | 99 | 187 | 148 | 212 | 241 | 405 | 372 | 290 | 268 | 306 | 303 | 359 | 451 | 260 | 161 | 201 | 224 | 156 | 264 | 161 | 379 | 375 | |
Labelled Cell Count | 201 | 110 | 200 | 161 | 240 | 271 | 474 | 421 | 378 | 338 | 376 | 340 | 425 | 547 | 319 | 186 | 231 | 224 | 156 | 264 | 161 | 379 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.1 | 5.3 | 4.9 | 5.9 | 4.9 | 5.3 | 5.7 | 4.7 | – | 4.5 | 5.4 | 5.2 | 5.7 | 5.8 | 9.6 | 9.8 | 10.0 | 4.7 | 4.8 | 5.4 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.9 | 0.9 | 1.2 | 0.9 | 1.0 | 1.1 | 1.0 | – | 0.9 | 1.0 | 0.8 | 1.0 | 1.0 | 1.3 | 1.8 | 1.8 | 1.1 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.15 | -0.12 | -0.0 | 0.09 | -0.19 | – | -0.25 | 0.0 | -0.03 | 0.09 | 0.12 | 0.84 | 0.88 | 0.91 | -0.19 | -0.14 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -2.9 | -5.0 | -5.6 | -5.7 | -8.3 | – | -8.8 | -6.9 | -6.0 | -6.9 | -6.6 | -5.1 | -6.3 | -6.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.7 | 5.3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.4 | 2.7 | 6.6 | 6.1 | 4.4 | 0 | – | 0 | 8.1 | 2.9 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.4 | 1.4 | 2.3 | 3.3 | 4.1 | 2.0 | – | 1.8 | 3.9 | 0 | 0 | 0 | 1.4 | 1.1 | 1.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.0 | 2.4 | 0.5 | 2.0 | 1.9 | 2.0 | – | -0.5 | 0.7 | 6.2 | 8.4 | 7.4 | 7.0 | 7.2 | 6.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5633 | 1.1289 | 0.885 | 1.0323 | 0.6472 | 1.0384 | 0.8064 | 1.0364 | 0.8391 | 0.676 | 0.7827 | 1.0026 | 0.8515 | 1.4433 | 0.8748 | 0.7897 | 0.8692 | 1.0429 |
Actin | 0.0339 | 0.0032 | 0.0147 | 0.0257 | 0.0091 | 0.0011 | 0.0352 | 0.0003 | 0.0021 | 0.0058 | 0.004 | 0.0036 | 0.0068 | 0.0016 | 0.0055 | 0.0011 | 0.0156 | 0.0016 |
Bud | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.001 | 0.0004 | 0.0008 | 0.0002 | 0.0006 | 0.0006 | 0.0041 | 0.0006 | 0.0005 | 0.0009 | 0.0004 | 0.0005 | 0.0008 | 0.0011 |
Bud Neck | 0.0034 | 0.0004 | 0.0012 | 0.0012 | 0.0066 | 0.0047 | 0.001 | 0.0005 | 0.0012 | 0.0017 | 0.0026 | 0.0057 | 0.0035 | 0.0005 | 0.0007 | 0.0049 | 0.0012 | 0.005 |
Bud Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.001 | 0.0003 | 0.0011 | 0.0001 | 0.0005 | 0.0008 | 0.0053 | 0.0009 | 0.0005 | 0.0006 | 0.0002 | 0.0003 | 0.0009 | 0.0013 |
Bud Site | 0.0014 | 0.0015 | 0.0013 | 0.0006 | 0.0028 | 0.0006 | 0.002 | 0.0012 | 0.0024 | 0.0009 | 0.0061 | 0.0016 | 0.0017 | 0.0024 | 0.0018 | 0.0014 | 0.0014 | 0.0005 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.4737 | 0.5644 | 0.5384 | 0.4933 | 0.4439 | 0.5403 | 0.404 | 0.6146 | 0.5437 | 0.4061 | 0.3977 | 0.5127 | 0.4235 | 0.6086 | 0.4465 | 0.4249 | 0.403 | 0.4553 |
Cytoplasmic Foci | 0.0209 | 0.0084 | 0.0075 | 0.0052 | 0.0119 | 0.0129 | 0.0276 | 0.0064 | 0.0096 | 0.0161 | 0.0243 | 0.0135 | 0.0371 | 0.0111 | 0.0107 | 0.0106 | 0.0269 | 0.0093 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0017 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0157 | 0.0177 | 0.0152 | 0.0035 | 0.0111 | 0.0103 | 0.0163 | 0.0079 | 0.01 | 0.0048 | 0.0073 | 0.0064 | 0.0105 | 0.0078 | 0.0046 | 0.0127 | 0.0083 | 0.0046 |
Endosome | 0.033 | 0.0085 | 0.0106 | 0.0057 | 0.0237 | 0.0192 | 0.0586 | 0.0118 | 0.0203 | 0.0134 | 0.0281 | 0.0184 | 0.0275 | 0.0065 | 0.0183 | 0.0156 | 0.0514 | 0.0132 |
Golgi | 0.0098 | 0.0037 | 0.001 | 0.0059 | 0.002 | 0.0009 | 0.0186 | 0.0003 | 0.0059 | 0.0069 | 0.0219 | 0.0046 | 0.0056 | 0.0013 | 0.0025 | 0.0004 | 0.0146 | 0.0025 |
Lipid Particles | 0.0044 | 0.0017 | 0.0007 | 0.0326 | 0.0011 | 0.0011 | 0.0163 | 0.0004 | 0.0014 | 0.0018 | 0.0496 | 0.0025 | 0.0074 | 0.001 | 0.0015 | 0.0022 | 0.0128 | 0.0027 |
Mitochondria | 0.0103 | 0.0043 | 0.001 | 0.0237 | 0.0014 | 0.0014 | 0.0141 | 0.0005 | 0.0024 | 0.0132 | 0.0744 | 0.0028 | 0.0075 | 0.0008 | 0.0115 | 0.0007 | 0.0483 | 0.0022 |
None | 0.1147 | 0.1588 | 0.1202 | 0.1407 | 0.1257 | 0.0813 | 0.1019 | 0.0751 | 0.0949 | 0.058 | 0.1309 | 0.0422 | 0.0879 | 0.0588 | 0.0787 | 0.1003 | 0.0324 | 0.0404 |
Nuclear Periphery | 0.0277 | 0.0167 | 0.0235 | 0.009 | 0.0224 | 0.0132 | 0.049 | 0.0185 | 0.0239 | 0.0121 | 0.0169 | 0.0138 | 0.0371 | 0.0172 | 0.016 | 0.0311 | 0.0194 | 0.0186 |
Nucleolus | 0.0013 | 0.0005 | 0.0016 | 0.0006 | 0.0019 | 0.0014 | 0.0017 | 0.0006 | 0.0009 | 0.0008 | 0.0026 | 0.0018 | 0.0009 | 0.0008 | 0.0013 | 0.0013 | 0.0007 | 0.001 |
Nucleus | 0.2202 | 0.1961 | 0.2428 | 0.194 | 0.3109 | 0.282 | 0.2097 | 0.2501 | 0.254 | 0.4044 | 0.1784 | 0.3398 | 0.3011 | 0.2554 | 0.3746 | 0.3442 | 0.3404 | 0.4217 |
Peroxisomes | 0.002 | 0.0044 | 0.0003 | 0.0458 | 0.0009 | 0.0003 | 0.0109 | 0.0002 | 0.0009 | 0.0048 | 0.0143 | 0.0024 | 0.017 | 0.0054 | 0.0022 | 0.0003 | 0.0051 | 0.0016 |
Punctate Nuclear | 0.0195 | 0.0058 | 0.0152 | 0.0072 | 0.0142 | 0.0216 | 0.0142 | 0.0069 | 0.0189 | 0.0446 | 0.0165 | 0.0207 | 0.0172 | 0.0154 | 0.0194 | 0.0423 | 0.0112 | 0.0135 |
Vacuole | 0.0051 | 0.0029 | 0.0034 | 0.0024 | 0.0073 | 0.0061 | 0.0098 | 0.0038 | 0.005 | 0.0023 | 0.0113 | 0.0045 | 0.0051 | 0.0034 | 0.0027 | 0.0042 | 0.0035 | 0.0028 |
Vacuole Periphery | 0.0013 | 0.0003 | 0.0007 | 0.0006 | 0.0011 | 0.0011 | 0.0066 | 0.0006 | 0.0012 | 0.0006 | 0.0026 | 0.0013 | 0.0015 | 0.0005 | 0.0006 | 0.0008 | 0.0017 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.6281 | 6.1704 | 7.5221 | 10.1213 | 9.9082 | 12.319 | 12.9233 | 16.2417 | 13.4235 | 11.245 |
Translational Efficiency | 0.7548 | 1.0875 | 0.8002 | 0.6503 | 0.6074 | 0.6743 | 0.6847 | 0.5004 | 0.7103 | 0.658 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1273 | 1247 | 1279 | 1400 | 749 | 2121 | 1797 | 3142 | 2022 | 3368 | 3076 | 4542 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 660.80 | 801.77 | 940.84 | 1004.91 | 709.77 | 821.65 | 942.20 | 1000.88 | 678.94 | 814.29 | 941.63 | 1002.12 |
Standard Deviation | 67.55 | 101.63 | 127.89 | 130.95 | 105.50 | 103.87 | 121.42 | 132.44 | 86.92 | 103.49 | 124.15 | 131.99 |
Intensity Change Log 2 | — | 0.278975 | 0.509736 | 0.604781 | — | 0.211172 | 0.408682 | 0.495846 | — | 0.244260 | 0.458288 | 0.549395 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000031 | 0.000295 | 0.000891 | 0.001684 | 0.000265 | 0.000195 | 0.000294 | 0.000269 | 0.000118 | 0.000232 | 0.000542 | 0.000705 |
Bud Neck | 0.001273 | 0.005832 | 0.010133 | 0.015236 | 0.003396 | 0.008035 | 0.005438 | 0.019519 | 0.002060 | 0.007219 | 0.007390 | 0.018199 |
Bud Site | 0.000640 | 0.004184 | 0.003663 | 0.020545 | 0.001757 | 0.004264 | 0.001316 | 0.009093 | 0.001054 | 0.004234 | 0.002292 | 0.012623 |
Cell Periphery | 0.000132 | 0.000129 | 0.000156 | 0.000262 | 0.000182 | 0.000085 | 0.000148 | 0.000103 | 0.000151 | 0.000101 | 0.000151 | 0.000152 |
Cytoplasm | 0.562630* | 0.434828* | 0.115587* | 0.132823* | 0.458689* | 0.431898* | 0.122144* | 0.186849* | 0.524127* | 0.432983* | 0.119417* | 0.170196* |
Cytoplasmic Foci | 0.110172 | 0.099656 | 0.002499 | 0.008891 | 0.129171 | 0.098612 | 0.003411 | 0.000683 | 0.117210 | 0.098998 | 0.003032 | 0.003213 |
Eisosomes | 0.000061 | 0.000055 | 0.000025 | 0.000042 | 0.000079 | 0.000047 | 0.000029 | 0.000010 | 0.000067 | 0.000050 | 0.000027 | 0.000020 |
Endoplasmic Reticulum | 0.000636 | 0.003337 | 0.009550 | 0.015040 | 0.003296 | 0.001662 | 0.004267 | 0.002326 | 0.001622 | 0.002282 | 0.006464 | 0.006245 |
Endosome | 0.002416 | 0.007279 | 0.001344 | 0.009605 | 0.005768 | 0.005951 | 0.000778 | 0.000763 | 0.003657 | 0.006443 | 0.001014 | 0.003489 |
Golgi | 0.000807 | 0.002536 | 0.000150 | 0.009045 | 0.001189 | 0.002360 | 0.000056 | 0.000232 | 0.000948 | 0.002425 | 0.000095 | 0.002949 |
Lipid Particles | 0.005410 | 0.007179 | 0.000764 | 0.001693 | 0.009563 | 0.006118 | 0.000715 | 0.000227 | 0.006948 | 0.006511 | 0.000735 | 0.000679 |
Mitochondria | 0.001808 | 0.004031 | 0.001786 | 0.009391 | 0.003143 | 0.007387 | 0.000935 | 0.003758 | 0.002302 | 0.006145 | 0.001289 | 0.005494 |
Mitotic Spindle | 0.000751 | 0.007096 | 0.007898 | 0.076641 | 0.003706 | 0.011544 | 0.003073 | 0.023422 | 0.001845 | 0.009897 | 0.005079 | 0.039826 |
None | 0.010223 | 0.006101 | 0.004961 | 0.006399 | 0.009343 | 0.003880 | 0.009688 | 0.009877 | 0.009897 | 0.004703 | 0.007723 | 0.008805 |
Nuclear Periphery | 0.000449 | 0.002179 | 0.003235 | 0.009514 | 0.001184 | 0.002602 | 0.002513 | 0.005385 | 0.000721 | 0.002446 | 0.002813 | 0.006657 |
Nuclear Periphery Foci | 0.000698 | 0.003289 | 0.002456 | 0.005831 | 0.004020 | 0.001092 | 0.000712 | 0.000320 | 0.001929 | 0.001905 | 0.001437 | 0.002019 |
Nucleolus | 0.001249 | 0.002503 | 0.001426 | 0.001271 | 0.003011 | 0.001889 | 0.001398 | 0.001000 | 0.001902 | 0.002116 | 0.001410 | 0.001083 |
Nucleus | 0.288451* | 0.375884* | 0.819472* | 0.625144* | 0.327567* | 0.382345* | 0.833080* | 0.724064* | 0.302941* | 0.379953* | 0.827422* | 0.693574* |
Peroxisomes | 0.000799 | 0.003575 | 0.000227 | 0.001150 | 0.003533 | 0.003891 | 0.000158 | 0.000138 | 0.001812 | 0.003774 | 0.000187 | 0.000450 |
Vacuole | 0.011028 | 0.029167 | 0.013368 | 0.045052 | 0.029938 | 0.025121 | 0.009735 | 0.011328 | 0.018033 | 0.026619 | 0.011246 | 0.021723 |
Vacuole Periphery | 0.000337 | 0.000865 | 0.000407 | 0.004740 | 0.001201 | 0.001021 | 0.000113 | 0.000634 | 0.000657 | 0.000963 | 0.000235 | 0.001899 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.05 | -16.43 | -16.75 | -12.40 | -2.12 | 1.48 | -3.67 | -0.97 | -3.32 | 3.76 | -3.79 | -15.15 | -15.99 | -11.00 | 2.69 |
Bud Neck | -5.32 | -10.55 | -19.60 | -9.84 | -3.10 | -3.85 | -3.86 | -12.47 | -10.37 | -11.67 | -7.73 | -11.59 | -22.39 | -13.78 | -10.57 |
Bud Site | -5.63 | -6.66 | -7.59 | -4.30 | -4.02 | -4.19 | -0.69 | -5.21 | -1.24 | -4.95 | -7.42 | -6.93 | -9.64 | -3.60 | -5.75 |
Cell Periphery | 0.16 | -3.06 | -4.33 | -5.55 | -1.56 | 8.65 | 4.05 | 10.61 | 1.54 | 7.87 | 5.51 | -0.08 | 4.69 | -1.42 | 6.16 |
Cytoplasm | 13.12 | 56.96 | 59.47* | 40.06 | -0.43 | 2.31 | 35.25 | 32.45 | 45.01 | -7.43 | 12.82 | 66.67 | 64.45 | 59.23 | -7.04 |
Cytoplasmic Foci | 1.85 | 28.67 | 28.29 | 22.68 | -1.68 | 4.71 | 23.18 | 23.62 | 30.25 | 6.28 | 4.41 | 36.80 | 37.23 | 37.90 | 3.19 |
Eisosomes | 0.75 | 9.15 | 0.98 | 0.14 | -9.56 | 7.56 | 10.77 | 16.19 | 14.55 | 9.44 | 6.23 | 13.68 | 17.24 | 12.66 | 3.33 |
Endoplasmic Reticulum | -12.97 | -21.78 | -24.15 | -18.89 | -4.71 | 5.32 | -8.20 | -2.13 | -13.40 | 8.09 | -4.57 | -23.56 | -22.98 | -21.17 | 3.65 |
Endosome | -6.66 | 3.52 | -2.77 | 4.15 | -5.08 | 0.07 | 11.89 | 12.68 | 12.10 | 3.41 | -6.19 | 11.19 | 7.96 | 12.00 | -2.84 |
Golgi | -1.86 | 1.96 | -1.53 | 0.33 | -2.56 | -1.97 | 4.12 | 3.84 | 4.33 | -1.79 | -2.84 | 3.63 | 0.69 | 3.31 | -2.70 |
Lipid Particles | -2.67 | 11.07 | 8.39 | 9.34 | -7.65 | 2.99 | 8.10 | 8.52 | 11.63 | 6.24 | 0.79 | 12.70 | 12.77 | 15.13 | 0.23 |
Mitochondria | -1.88 | -0.27 | -2.90 | -0.81 | -4.25 | -3.43 | 2.15 | -0.55 | 4.19 | -14.37 | -4.37 | 1.24 | -2.60 | 3.20 | -10.40 |
Mitotic Spindle | -3.46 | -5.04 | -11.48 | -9.54 | -9.21 | -3.55 | -0.89 | -8.76 | -5.12 | -8.95 | -5.71 | -4.48 | -15.51 | -10.23 | -12.55 |
None | 5.15 | 7.92 | 4.67 | -0.94 | -4.52 | 4.34 | 1.05 | -0.82 | -14.24 | -2.90 | 7.76 | 4.47 | 1.14 | -12.15 | -5.00 |
Nuclear Periphery | -9.96 | -17.19 | -22.10 | -17.44 | -13.72 | -5.94 | -11.49 | -19.38 | -13.98 | -11.95 | -10.86 | -20.65 | -28.57 | -20.65 | -17.06 |
Nuclear Periphery Foci | -5.60 | -5.12 | -7.51 | -3.07 | -4.03 | 5.69 | 5.10 | 6.86 | 3.84 | 4.05 | 0.08 | 0.15 | -0.84 | -0.95 | -1.01 |
Nucleolus | -3.87 | -2.04 | -3.36 | 1.76 | 0.03 | 2.12 | 2.81 | 0.75 | -2.86 | -4.58 | -0.83 | 0.77 | -1.92 | -1.34 | -3.31 |
Nucleus | -8.58 | -61.17 | -37.30 | -26.49 | 16.66 | -4.93 | -49.12 | -41.99 | -50.85 | 12.73 | -10.65 | -81.12 | -64.04* | -57.09 | 19.55 |
Peroxisomes | -5.94 | 4.99 | 2.99 | 6.89 | -1.64 | -0.26 | 2.98 | 3.05 | 9.11 | 2.78 | -3.66 | 3.83 | 3.87 | 11.49 | 0.60 |
Vacuole | -10.47 | -5.49 | -19.06 | -12.81 | -16.79 | 2.08 | 6.10 | 3.67 | 2.27 | -3.48 | -6.97 | 1.01 | -13.32 | -7.71 | -14.47 |
Vacuole Periphery | -4.76 | -0.10 | -2.32 | -0.93 | -2.19 | 0.50 | 3.42 | 2.74 | 4.84 | -2.40 | -2.05 | 3.28 | 0.39 | 2.46 | -2.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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