Standard name
Human Ortholog
Description [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.93 0.88 0.9 0.88 0.73 0.75 0.63 0.65 0.63 0.75 0.97 0.96 0.98 0.9 0.97 0.91 0.97 0.91 0.95 0.83 0.92 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.05 0.12 0.16 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.17 0.11 0.09 0.17 0.13 0.24 0.18 0.23 0.18 0 0.06 0 0.11 0 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 1 0 0 1 0 0 1
Bud 1 0 0 0 0 0 2 0 3 3 2 1 0 1 0 0 1 1 0 0 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 1 3 4 5 4 6 3 4 0 0 1 3 5 4 0 0 0 0 0 0
Cytoplasm 171 145 142 99 130 146 150 165 136 144 76 119 190 228 259 264 243 190 169 132 141 80 145 173
Endoplasmic Reticulum 0 0 1 1 0 0 3 13 3 9 2 4 0 1 2 9 5 4 0 0 0 1 0 0
Endosome 0 0 2 0 0 0 1 1 0 0 0 0 0 0 0 0 2 2 0 0 1 0 1 4
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 3 1 0 0 0 0 0 0
Mitochondria 0 1 1 0 0 5 17 11 25 36 15 15 1 0 1 0 0 1 0 0 0 0 0 0
Nucleus 1 0 1 1 0 1 4 6 6 0 4 6 4 8 6 2 1 1 1 5 1 9 4 3
Nuclear Periphery 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 1 2 1 1 2 0 1 0 0 1 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 1 8 19 16 15 36 29 51 39 28 28 4 14 10 31 4 21 0 1 0 2 1 0
Unique Cell Count 172 146 152 113 145 166 206 220 216 220 120 159 195 238 265 292 251 209 174 146 150 97 158 186
Labelled Cell Count 173 147 158 122 147 171 219 235 231 240 130 179 199 252 280 311 264 228 174 146 150 97 158 186


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.5 16.0 15.1 13.1 13.6 12.3 11.9 12.4 11.4 11.9 10.9 11.0 16.1 15.7 14.5 23.7 23.8 22.1 13.4 15.6 17.8
Std Deviation (1e-4) 2.4 3.4 2.8 2.8 2.7 2.7 2.3 3.0 2.4 3.1 2.6 3.0 3.1 3.2 2.9 7.5 6.9 7.0 3.1 3.1 3.5
Intensity Change (Log2) – – – -0.2 -0.15 -0.3 -0.35 -0.28 -0.41 -0.34 -0.47 -0.46 0.09 0.06 -0.06 0.65 0.66 0.55 -0.17 0.05 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.2 -1.7 -5.0 -4.6 -6.7 -6.3 -6.2 -4.5 1.8 1.0 2.2 -1.1 1.6 -0.9
Endoplasmic Reticulum 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.3 0 4.0 5.0 4.1 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.1 1.8 1.3 3.5 2.5 4.7 3.6 4.4 3.4 -1.6 0.3 -0.7 1.9 0 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.4437 10.7552 9.7332 10.056 8.4884 10.642 7.2842 10.0442 9.5801 8.6108 8.3553 8.9155 8.9851 10.8473 9.6721 8.7845 8.2566 8.9567
Actin 0.0233 0.0011 0.0099 0.0003 0.014 0.0004 0.0491 0.0004 0.0006 0.0002 0.0096 0.0003 0.0019 0.0012 0.0008 0.0002 0.0006 0.0016
Bud 0.0003 0.0002 0.0004 0.0001 0.0004 0.0004 0.0029 0.0001 0.0005 0.0002 0.0004 0.0001 0.0009 0.0003 0.0003 0.0001 0.0001 0.0008
Bud Neck 0.0013 0.0004 0.0006 0.0008 0.0008 0.0017 0.0066 0.0001 0.0002 0.0005 0.0007 0.0007 0.0003 0.0003 0.0006 0.0006 0.0009 0.0011
Bud Periphery 0.0002 0.0001 0.0002 0 0.0003 0.0001 0.0034 0 0.0001 0 0.0003 0 0.0002 0.0001 0.0004 0 0 0.0002
Bud Site 0.0007 0.0046 0.0013 0.0001 0.0005 0.0004 0.038 0.0006 0.0002 0.0001 0.0007 0 0.0008 0.0022 0.0004 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.002 0 0 0 0 0 0 0 0.0001 0 0 0
Cytoplasm 0.8479 0.8936 0.8748 0.9258 0.785 0.8727 0.6745 0.9879 0.9796 0.9852 0.7892 0.964 0.9148 0.9515 0.9169 0.9047 0.9304 0.8975
Cytoplasmic Foci 0.0161 0.0292 0.0074 0.0009 0.0074 0.0041 0.0565 0.0008 0.0008 0.0019 0.0603 0.0012 0.0109 0.0055 0.008 0.0187 0.0031 0.0042
Eisosomes 0.0003 0 0 0 0.0001 0 0.0005 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0064 0.001 0.0027 0.0009 0.0082 0.0027 0.0114 0.0004 0.0008 0.0003 0.0011 0.0005 0.0024 0.0008 0.0013 0.0013 0.0014 0.0017
Endosome 0.0138 0.0025 0.0052 0.0005 0.0138 0.002 0.0531 0.0001 0.0002 0.0005 0.0487 0.0004 0.0076 0.0013 0.0076 0.0073 0.0075 0.004
Golgi 0.0042 0.0005 0.0011 0 0.0102 0.0005 0.0219 0 0 0 0.0633 0 0.0012 0.0002 0.0011 0.0003 0.0007 0.0009
Lipid Particles 0.0061 0.0003 0.0015 0 0.0087 0.0001 0.0188 0 0 0 0.0049 0 0.0009 0 0.0018 0.0012 0.0031 0.0009
Mitochondria 0.0025 0.0201 0.0019 0.0001 0.0021 0.0068 0.0073 0.0001 0.0001 0.0001 0.0022 0.0001 0.0014 0.0002 0.0011 0.0001 0.0005 0.0027
None 0.0041 0.0121 0.011 0.0011 0.0462 0.0156 0.0061 0.0013 0.0013 0.0009 0.0005 0.0008 0.0055 0.0013 0.0016 0.0018 0.0021 0.0015
Nuclear Periphery 0.0093 0.002 0.0085 0.0029 0.0083 0.0038 0.009 0.0006 0.001 0.0007 0.0015 0.0015 0.0068 0.0018 0.0033 0.0029 0.0118 0.0084
Nucleolus 0.0002 0.0027 0.0003 0 0.0001 0.0046 0.0006 0 0 0 0 0 0.0001 0 0.0001 0 0.0009 0.0001
Nucleus 0.0505 0.0229 0.065 0.0654 0.0849 0.0795 0.0125 0.0072 0.0141 0.0085 0.0086 0.0295 0.0384 0.0306 0.05 0.0578 0.0288 0.0693
Peroxisomes 0.0076 0.0014 0.0016 0 0.0034 0.0001 0.0144 0 0 0.0001 0.0062 0 0.0027 0.0006 0.0024 0.0004 0.0003 0.002
Punctate Nuclear 0.0029 0.0051 0.0051 0.0007 0.0027 0.0035 0.0045 0.0002 0.0002 0.0001 0.0009 0.0005 0.0017 0.0016 0.001 0.002 0.0065 0.002
Vacuole 0.0016 0.0002 0.0011 0.0003 0.0023 0.0006 0.0054 0.0001 0.0002 0.0004 0.0004 0.0002 0.0008 0.0004 0.001 0.0004 0.0007 0.0005
Vacuole Periphery 0.0005 0.0001 0.0004 0.0001 0.0005 0.0002 0.0017 0 0 0.0001 0.0002 0.0001 0.0007 0.0001 0.0003 0.0001 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.3374 59.1178 44.4689 63.4016 76.9336 57.041 65.301 64.3836 56.686 69.451
Translational Efficiency 3.1714 2.8564 3.5359 1.9821 2.4367 3.7919 2.096 2.4502 2.5965 2.623

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 240 2319 1356 1275 1670 201 1460 1358 1910 2520 2816

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1389.34 1414.47 1612.81 1954.17 1323.92 1439.09 1810.57 1838.02 1327.92 1436.00 1628.58 1893.95
Standard Deviation 220.51 279.68 265.96 410.62 221.31 221.33 287.64 409.21 221.82 229.62 273.06 413.98
Intensity Change Log 2 — 0.025862 0.215177 0.492156 — 0.120341 0.451628 0.473337 — 0.072736 0.335394 0.483004

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000489 0.000396 0.000128 0.003825 0.000292 0.000329 0.000639 0.001224 0.000304 0.000338 0.000169 0.002477
Bud Neck 0.001030 0.000574 0.002089 0.004441 0.000639 0.000640 0.003180 0.001726 0.000663 0.000631 0.002176 0.003033
Bud Site 0.005390 0.003046 0.002768 0.024560 0.002278 0.003720 0.005177 0.015042 0.002468 0.003636 0.002960 0.019625
Cell Periphery 0.000220 0.000128 0.000070 0.000407 0.000115 0.000070 0.000245 0.000120 0.000121 0.000078 0.000084 0.000258
Cytoplasm 0.796466* 0.788212* 0.585363* 0.515293* 0.793328* 0.792053* 0.489902* 0.667463* 0.793520* 0.791570* 0.577749* 0.594188*
Cytoplasmic Foci 0.050235 0.022843 0.003714 0.016056 0.015761 0.021787 0.006517 0.008740 0.017868 0.021920 0.003938 0.012263
Eisosomes 0.000013 0.000007 0.000004 0.000021 0.000004 0.000005 0.000015 0.000006 0.000005 0.000005 0.000005 0.000013
Endoplasmic Reticulum 0.001589 0.000754 0.000828 0.001020 0.001458 0.000796 0.001387 0.000141 0.001466 0.000790 0.000872 0.000564
Endosome 0.001571 0.001970 0.002128 0.008578 0.000741 0.001954 0.001478 0.002591 0.000791 0.001956 0.002076 0.005474
Golgi 0.000582 0.001325 0.000362 0.027512 0.000856 0.003785 0.000261 0.003433 0.000840 0.003476 0.000354 0.015028
Lipid Particles 0.001067 0.000511 0.000429 0.004646 0.000313 0.000645 0.001413 0.000643 0.000359 0.000628 0.000507 0.002571
Mitochondria 0.000052 0.000066 0.000458 0.002606 0.000135 0.000519 0.000402 0.000171 0.000130 0.000462 0.000454 0.001344
Mitotic Spindle 0.001409 0.000484 0.010844 0.066167 0.002605 0.010558 0.007957 0.020554 0.002532 0.009293 0.010614 0.042518
None 0.002093 0.006596 0.010769 0.010143 0.007554 0.008353 0.013935 0.015780 0.007220 0.008132 0.011022 0.013066
Nuclear Periphery 0.000175 0.000119 0.000717 0.000574 0.000164 0.000139 0.001142 0.000140 0.000164 0.000136 0.000750 0.000349
Nuclear Periphery Foci 0.008051 0.006202 0.001109 0.002865 0.002614 0.002796 0.004799 0.001381 0.002946 0.003224 0.001403 0.002096
Nucleolus 0.000073 0.000085 0.000182 0.002242 0.000082 0.000088 0.000224 0.000107 0.000081 0.000088 0.000185 0.001135
Nucleus 0.089205 0.136675* 0.350703* 0.260967* 0.161173* 0.133896* 0.364054* 0.249705* 0.156774* 0.134245* 0.351768* 0.255128*
Peroxisomes 0.000119 0.000094 0.000090 0.001775 0.000049 0.000164 0.000113 0.000377 0.000053 0.000155 0.000092 0.001050
Vacuole 0.040042 0.029681 0.026868 0.032403 0.008975 0.016165 0.097016 0.010513 0.010874 0.017864 0.032463 0.021054
Vacuole Periphery 0.000129 0.000232 0.000376 0.013897 0.000865 0.001537 0.000145 0.000142 0.000820 0.001373 0.000357 0.006766

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.31 2.05 -2.07 -2.59 -3.91 -0.40 -4.98 -1.17 -1.02 1.69 -0.52 0.18 -3.14 -2.92 -3.33
Bud Neck 2.36 -5.26 -6.30 -10.11 -1.18 -0.98 -6.28 -5.23 -4.94 4.80 -0.49 -10.98 -10.89 -10.83 2.35
Bud Site 2.06 3.29 -1.90 -4.08 -5.25 -3.22 -4.92 -4.77 -3.41 0.52 -3.04 -2.57 -7.17 -5.41 -5.71
Cell Periphery 1.73 2.51 -2.86 -7.62 -9.85 2.69 -6.68 -3.29 -9.23 5.38 2.66 -0.70 -7.73 -13.01 -9.16
Cytoplasm -0.14 11.89 11.02 18.39 -1.93 1.56 25.43 20.44 19.71 -15.65 1.62 44.04 32.22 32.67 -13.47
Cytoplasmic Foci 2.71 5.10 4.39 3.50 -8.09 -3.44 4.13 4.52 8.85 -0.23 -2.43 9.43 5.44 9.22 -9.73
Eisosomes 2.02 2.50 1.09 -2.23 -4.45 -1.57 -10.06 -1.36 -0.41 8.88 -1.08 -5.95 -4.46 -4.11 -0.05
Endoplasmic Reticulum 1.71 1.75 2.84 2.69 3.55 1.98 -0.33 4.05 4.03 8.48 2.13 1.93 3.99 3.82 5.50
Endosome -0.02 1.08 1.20 0.57 -0.01 -2.76 -4.19 0.52 2.66 3.53 -2.73 -1.17 -0.29 2.32 0.91
Golgi -0.64 1.26 -2.38 -1.59 -2.71 -2.78 1.74 0.16 2.62 -0.87 -2.79 2.16 -1.73 1.02 -2.90
Lipid Particles 1.92 1.31 -3.05 -6.97 -6.54 -2.41 -8.61 -8.06 -2.57 5.47 -2.10 -9.00 -9.78 -6.56 -5.84
Mitochondria -0.88 -20.24 -3.28 -3.21 0.77 -2.42 -10.78 -1.28 2.28 10.21 -2.37 -18.46 -2.85 0.42 3.96
Mitotic Spindle 1.25 -7.53 -7.16 -7.96 -2.10 -3.96 -2.97 -3.00 0.97 0.99 -3.80 -7.78 -7.23 -2.49 0.16
None -3.36 -11.96 -9.38 -2.68 3.35 -0.30 -1.65 -3.79 -3.85 -0.38 -0.45 -4.42 -3.70 -3.75 0.90
Nuclear Periphery 2.26 -20.68 -13.59 -20.94 10.08 0.65 -12.80 -6.79 -7.88 11.91 1.09 -27.70 -18.07 -20.11 18.73
Nuclear Periphery Foci 0.83 2.69 2.49 5.31 -4.03 -0.86 -3.45 5.31 5.24 5.72 -0.97 4.88 4.95 6.29 -0.26
Nucleolus -0.71 -7.30 -1.48 -1.42 -0.85 -1.27 -5.47 -2.51 -1.66 4.76 -1.39 -9.67 -1.60 -1.51 -0.24
Nucleus -3.83 -27.69 -16.21 -12.62 17.29 4.71 -11.93 -12.69 -18.64 6.35 4.04 -37.48 -17.91 -25.62 22.56
Peroxisomes 1.29 2.12 -1.13 -1.37 -1.51 -3.73 -3.80 -1.81 0.02 -0.14 -3.74 -1.87 -2.20 -1.02 -1.77
Vacuole 1.43 0.29 -5.07 -12.52 -14.21 -7.85 -12.11 -17.48 -12.43 8.13 -6.80 -19.54 -28.13 -22.77 -7.49
Vacuole Periphery -0.60 -1.36 -1.88 -1.77 -1.78 -0.50 1.03 1.19 2.72 5.71 -0.43 0.97 0.05 0.66 -1.56
Standard name
Human Ortholog
Description [PIN(+)] prion; an infectious protein conformation that is generally an ordered protein aggregate
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Rnq1

Rnq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available