Standard name
Human Ortholog
Description Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.09 0.06 0.08 0.07 0.06 0.09 0.12 0.13 0.07 0.06 0.13 0.17 0.37 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.1 0 0.08 0.08 0.21 0.27 0.26 0.43 0.35 0.43 0.51 0 0 0 0.11 0.09 0.15 0 0 0 0 0 0
Nucleus 0.93 0.98 0.9 0.87 0.87 0.79 0.76 0.76 0.74 0.65 0.63 0.73 0.93 0.86 0.82 0.68 0.83 0.9 0.92 0.9 0.79 0.85 0.84 0.82
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0.08 0.08 0 0 0.07 0.07 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 1
Bud 1 0 0 0 0 5 9 5 2 2 3 7 0 0 1 0 0 0 3 0 0 2 3 5
Bud Neck 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Bud Site 0 0 1 1 6 7 13 12 4 4 2 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
Cytoplasm 4 0 14 18 22 25 39 29 21 42 34 24 25 82 119 7 5 3 0 0 2 3 7 13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 2 2 2 0 0 1 0 1 0
Endosome 0 0 1 1 3 2 2 7 1 5 3 2 1 2 3 0 1 0 1 1 1 1 2 5
Golgi 0 0 0 0 2 0 0 0 0 0 1 0 0 0 1 0 1 0 1 0 1 0 0 2
Mitochondria 83 9 8 17 29 66 142 122 102 119 110 184 3 2 1 2 7 7 0 0 6 1 8 7
Nucleus 296 86 238 183 321 253 395 351 175 220 162 265 379 551 579 13 65 43 284 86 221 141 403 439
Nuclear Periphery 0 0 4 8 0 9 11 8 3 3 5 16 2 12 2 0 1 1 0 0 1 0 1 0
Nucleolus 3 5 7 6 15 12 19 23 10 13 9 10 4 3 9 0 1 1 3 0 7 0 4 3
Peroxisomes 0 0 1 0 0 1 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 1 2 2 0 4 6 1 1 0 4 2 6 12 0 0 0 1 1 2 0 4 3
Vac/Vac Membrane 2 2 7 2 7 8 20 19 4 22 21 10 3 3 7 0 6 4 10 3 18 12 36 47
Unique Cell Count 319 88 265 211 369 319 523 462 238 336 257 364 407 643 710 19 78 48 310 96 280 167 483 538
Labelled Cell Count 391 102 282 238 407 388 657 583 323 433 350 536 419 662 740 24 89 61 310 96 280 167 483 538


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.1 6.0 6.0 5.7 5.1 5.0 4.7 4.5 5.0 4.5 4.1 6.8 6.5 6.4 7.8 7.0 6.8 5.9 6.3 6.9
Std Deviation (1e-4) 0.7 0.8 2.4 3.5 2.6 3.9 3.9 2.3 2.5 3.5 2.7 1.9 1.6 1.4 1.4 2.1 1.8 1.2 1.1 1.3 1.6
Intensity Change (Log2) – – – 0.01 -0.08 -0.22 -0.26 -0.35 -0.4 -0.26 -0.4 -0.55 0.18 0.13 0.09 0.39 0.23 0.19 -0.02 0.08 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.4 1.2 1.2 0.5 1.6 3.0 3.1 0.7 0.5 3.3 4.6 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 2.6 6.4 8.2 0 0 9.7 0 0 0 0 0 0 0 0
Nucleus -1.0 -1.1 -3.5 -4.8 -4.6 -4.8 -7.0 -7.2 -5.3 1.5 -1.7 -3.1 -2.8 -1.6 0
Nuclear Periphery 1.6 0 1.1 0.6 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 1.0 0.8 0.7 0 0 0.8 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.6 -0.1 0.9 1.0 -0.7 2.2 2.8 0.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2183 0.9736 0.9023 0.9506 0.8375 0.93 0.7506 1.1998 0.9728 0.805 0.6447 0.8901 -0.2671 0.1874 -0.2052 0.4039 0.093 -0.3352
Actin 0.0309 0.0011 0.0045 0.0083 0.0165 0.0025 0.022 0.0016 0.0162 0.0037 0.0166 0.0034 0.0127 0.0001 0.0013 0.0628 0.0064 0.0003
Bud 0.0007 0.0001 0.0021 0.0003 0.0006 0.0046 0.0011 0.0001 0.0007 0.0004 0.0005 0.0006 0.0001 0 0 0.0001 0.0002 0.0001
Bud Neck 0.0035 0.0002 0.0004 0.0013 0.0021 0.0019 0.0141 0.0001 0.005 0.0005 0.0004 0.0006 0.0005 0.0001 0.0002 0.0002 0.0037 0.0023
Bud Periphery 0.0008 0.0001 0.0013 0.0008 0.0006 0.0013 0.002 0 0.0013 0.0006 0.001 0.0015 0.0002 0 0 0.0002 0.0002 0.0004
Bud Site 0.0015 0.0033 0.0026 0.0013 0.0014 0.0006 0.0069 0.0015 0.0048 0.0016 0.0007 0.0003 0.0003 0.0001 0.0001 0.0002 0.0005 0.0002
Cell Periphery 0.0003 0 0.0002 0.0002 0.0002 0.0001 0.0004 0 0.0003 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001
Cytoplasm 0.0135 0.0101 0.0096 0.0047 0.0044 0.0034 0.0084 0.0019 0.0037 0.0112 0.0005 0.0006 0.0043 0.0011 0.0005 0.0001 0.0026 0.0002
Cytoplasmic Foci 0.0178 0.003 0.0037 0.0053 0.0033 0.0012 0.0099 0.0021 0.012 0.0097 0.0051 0.0016 0.0028 0 0.0003 0.0004 0.0018 0.0001
Eisosomes 0.0003 0 0.0001 0 0.0004 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0042 0.0001 0.0011 0.0044 0.0045 0.0008 0.0031 0.0003 0.0007 0.0021 0.0009 0.0006 0.0022 0.0001 0.0004 0.0002 0.0016 0.0001
Endosome 0.0239 0.0007 0.0097 0.027 0.0266 0.0059 0.0315 0.0029 0.0109 0.0429 0.0228 0.0069 0.013 0.0001 0.0024 0.0013 0.0051 0.0008
Golgi 0.0043 0.0006 0.0027 0.0028 0.0038 0.001 0.0079 0.0011 0.0081 0.01 0.0163 0.0017 0.0029 0 0.0003 0.0023 0.0007 0.0004
Lipid Particles 0.0056 0.0003 0.0059 0.0038 0.0191 0.0013 0.0069 0.0008 0.0123 0.0127 0.0421 0.0038 0.0037 0 0.0002 0.0006 0.0009 0.0004
Mitochondria 0.0097 0.0002 0.0032 0.0059 0.0045 0.0029 0.0201 0.0004 0.0183 0.0114 0.0152 0.0043 0.0042 0.0002 0.0003 0.001 0.0008 0.003
None 0.0042 0.0028 0.0078 0.0011 0.0047 0.002 0.0158 0.0003 0.0004 0.0043 0.0005 0.0002 0.0068 0.0002 0.0002 0.0002 0.0007 0
Nuclear Periphery 0.0454 0.0048 0.0097 0.0173 0.0381 0.0125 0.0176 0.0028 0.0087 0.0038 0.0129 0.0039 0.0084 0.0103 0.0027 0.001 0.0093 0.0011
Nucleolus 0.0155 0.0123 0.0138 0.0115 0.0172 0.0163 0.0115 0.0062 0.01 0.0028 0.0115 0.0089 0.0065 0.006 0.0094 0.0026 0.0086 0.0099
Nucleus 0.7876 0.9352 0.9022 0.8878 0.8176 0.9265 0.7978 0.9728 0.8726 0.8709 0.8346 0.9557 0.9201 0.9802 0.9804 0.9254 0.9408 0.9777
Peroxisomes 0.0068 0.0009 0.0014 0.001 0.0016 0.0004 0.0034 0.0003 0.0077 0.0031 0.007 0.002 0.001 0 0.0001 0.0006 0.0005 0.0002
Punctate Nuclear 0.0173 0.0239 0.0118 0.0061 0.0275 0.0136 0.0135 0.0042 0.0039 0.0022 0.0088 0.0018 0.0084 0.0013 0.0009 0.0004 0.0146 0.0012
Vacuole 0.0037 0.0003 0.0042 0.0061 0.0027 0.0008 0.0039 0.0004 0.0013 0.0038 0.0012 0.0008 0.0007 0.0002 0.0002 0 0.0006 0.001
Vacuole Periphery 0.0025 0.0001 0.0021 0.0032 0.0026 0.0008 0.0021 0.0002 0.0009 0.0021 0.0013 0.0006 0.001 0.0002 0.0001 0.0001 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0323 9.4927 8.9651 8.9331 6.2581 9.6482 13.3064 10.306 11.6815 9.1355
Translational Efficiency 0.7822 0.9539 0.8977 0.9061 1.4066 1.0072 0.8321 0.927 0.7579 1.0184

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
927 188 182 979 1436 1547 66 969 2363 1735 248 1948

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 718.56 879.94 989.64 882.22 763.66 812.42 961.23 927.93 745.97 819.74 982.08 904.96
Standard Deviation 105.16 348.17 102.22 128.68 96.28 108.25 133.49 130.50 102.26 155.00 112.11 131.59
Intensity Change Log 2 — 0.292297 0.461795 0.296030 — 0.089295 0.331951 0.281086 — 0.191277 0.396358 0.288350

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000224 0.000757 0.001339 0.000760 0.000169 0.000233 0.001091 0.000681 0.000191 0.000290 0.001273 0.000721
Bud Neck 0.003893 0.012637 0.006537 0.018906 0.004117 0.004206 0.004222 0.018626 0.004029 0.005120 0.005921 0.018766
Bud Site 0.000779 0.003694 0.001713 0.005479 0.000910 0.001661 0.000804 0.003925 0.000858 0.001881 0.001471 0.004706
Cell Periphery 0.000174 0.000158 0.000137 0.000141 0.000117 0.000128 0.000070 0.000156 0.000139 0.000132 0.000119 0.000148
Cytoplasm 0.018982 0.008633 0.003031 0.020801 0.018432 0.010194 0.003725 0.020653 0.018648 0.010025 0.003216 0.020728
Cytoplasmic Foci 0.001718 0.007076 0.000187 0.000828 0.001515 0.001486 0.000126 0.000315 0.001594 0.002092 0.000171 0.000573
Eisosomes 0.000021 0.000034 0.000057 0.000026 0.000018 0.000016 0.000038 0.000025 0.000019 0.000018 0.000052 0.000026
Endoplasmic Reticulum 0.003366 0.009514 0.011652 0.009859 0.003192 0.003287 0.007564 0.008387 0.003260 0.003962 0.010564 0.009127
Endosome 0.000745 0.001818 0.000598 0.003112 0.000602 0.002378 0.000269 0.001476 0.000659 0.002318 0.000510 0.002298
Golgi 0.000541 0.003945 0.000094 0.000894 0.000205 0.000449 0.000076 0.000658 0.000337 0.000828 0.000089 0.000777
Lipid Particles 0.001107 0.000653 0.000517 0.000582 0.000652 0.000771 0.000351 0.000397 0.000831 0.000758 0.000473 0.000490
Mitochondria 0.000944 0.001814 0.001030 0.005960 0.001526 0.002305 0.000802 0.006836 0.001298 0.002252 0.000969 0.006396
Mitotic Spindle 0.000664 0.008734 0.000264 0.013387 0.000714 0.004070 0.000098 0.009955 0.000695 0.004576 0.000220 0.011680
None 0.013055 0.004748 0.003538 0.010441 0.004057 0.004439 0.001056 0.005452 0.007587 0.004472 0.002877 0.007960
Nuclear Periphery 0.000940 0.001002 0.000667 0.002634 0.001824 0.001494 0.000462 0.002448 0.001477 0.001441 0.000612 0.002541
Nuclear Periphery Foci 0.000731 0.000522 0.000328 0.001981 0.000702 0.000411 0.000374 0.000702 0.000713 0.000423 0.000340 0.001345
Nucleolus 0.014778 0.010998 0.006149 0.003735 0.012047 0.007659 0.006221 0.002561 0.013119 0.008021 0.006168 0.003151
Nucleus 0.935965* 0.918456* 0.952474* 0.887567* 0.946308* 0.950625* 0.969026* 0.901321* 0.942250* 0.947140* 0.956879* 0.894409*
Peroxisomes 0.000411 0.000693 0.000430 0.000326 0.000736 0.000509 0.000396 0.000166 0.000609 0.000529 0.000421 0.000246
Vacuole 0.000800 0.003476 0.009071 0.009080 0.001879 0.002457 0.003057 0.012202 0.001455 0.002568 0.007470 0.010633
Vacuole Periphery 0.000162 0.000636 0.000186 0.003500 0.000279 0.001219 0.000172 0.003057 0.000233 0.001156 0.000182 0.003280

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.50 -8.39 -11.78 -0.67 4.82 -1.74 -5.24 -12.46 -11.61 1.78 -3.49 -9.87 -17.13 -13.74 5.03
Bud Neck -3.96 -4.93 -10.47 -2.07 -6.86 -0.26 -2.24 -9.83 -9.58 -4.09 -2.18 -5.46 -14.41 -12.53 -8.31
Bud Site -2.21 -5.07 -4.77 -0.59 -2.96 -1.11 -1.77 -5.35 -3.36 -3.87 -1.81 -5.22 -6.91 -3.91 -4.31
Cell Periphery 0.27 0.69 1.43 1.00 0.49 -0.29 1.68 -0.94 -0.36 -2.36 0.32 0.35 0.25 -0.19 -0.19
Cytoplasm 3.14 6.00 -0.64 -4.22 -8.21 3.63 7.32 0.50 -3.16 -6.96 4.50 9.73 -0.28 -5.06 -10.90
Cytoplasmic Foci -1.46 3.84 2.71 1.77 -1.90 0.22 5.39 4.76 2.99 -3.43 -0.74 6.16 4.99 3.01 -2.09
Eisosomes -3.72 -6.68 -5.34 0.50 5.26 2.03 -4.43 -9.07 -11.61 1.68 1.72 -7.83 -10.40 -12.24 5.36
Endoplasmic Reticulum -4.72 -9.16 -10.04 -0.42 2.45 -0.20 -4.27 -12.70 -13.74 -1.21 -2.49 -10.09 -15.84 -14.41 1.59
Endosome -1.48 -0.26 -2.05 0.01 -2.08 -2.64 2.80 -2.22 1.64 -3.37 -2.75 -0.22 -2.84 0.98 -2.80
Golgi -1.11 0.84 -0.01 1.12 -1.72 -1.20 1.45 -0.66 0.35 -1.27 -1.17 1.08 -0.46 0.93 -2.02
Lipid Particles 1.03 0.73 1.16 0.07 0.84 -0.39 2.19 1.10 1.13 -1.67 0.33 0.81 1.43 0.89 0.80
Mitochondria -1.56 -1.73 -3.18 -2.01 -2.89 -1.10 0.95 -2.25 -1.63 -2.72 -1.71 0.18 -3.63 -2.31 -3.89
Mitotic Spindle -1.37 -0.72 -5.41 -1.50 -5.20 -2.61 2.58 -4.37 -2.78 -4.64 -2.98 -0.29 -6.95 -4.30 -6.77
None 3.29 4.26 2.03 -1.56 -2.66 -0.44 6.23 -0.29 0.20 -5.60 2.81 4.21 1.28 -1.54 -3.14
Nuclear Periphery -0.99 0.30 -5.36 -3.43 -5.72 0.59 4.12 -4.39 -3.35 -10.37 0.08 3.50 -6.19 -3.30 -10.56
Nuclear Periphery Foci 0.40 0.28 -1.58 -1.80 -1.76 1.48 1.68 -0.15 -2.64 -2.55 1.80 1.00 -1.36 -2.99 -2.29
Nucleolus 1.20 2.17 5.46 3.14 2.05 2.97 3.02 7.38 5.20 1.24 4.01 2.68 8.74 4.76 2.66
Nucleus 1.65 -0.11 6.60 2.22 5.95 -0.91 -1.97 8.53 8.92 8.58 -1.04 -0.08 10.94 10.85 8.53
Peroxisomes -1.14 -1.45 1.59 1.84 2.62 0.67 0.94 1.74 3.00 1.73 0.40 0.15 1.96 2.88 3.42
Vacuole -2.50 -3.69 -7.50 -4.14 -0.11 -0.80 -2.04 -8.67 -8.46 -6.05 -2.09 -3.83 -11.25 -10.19 -2.39
Vacuole Periphery -1.73 -1.20 -2.50 -1.73 -2.40 -2.44 1.41 -1.86 -0.28 -2.06 -2.70 0.34 -3.09 -0.94 -3.13
Standard name
Human Ortholog
Description Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Dcc1

Dcc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dcc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available