Standard name
Human Ortholog
Description Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.07 0.05 0.07 0.07 0 0.06 0.05 0 0.11 0.12 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.11 0 0 0 0 0 0 0.1 0.09 0.12 0.14 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.96 0.86 0.93 0.9 0.91 0.86 0.84 0.81 0.73 0.79 0.69 0.67 0.78 0.86 0.81 0.91 0.89 0.87 0.83 0.8 0.84 0.79 0.76 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 0.21 0.09 0.11 0.05 0.1 0.14 0.15 0.4 0.31 0.52 0.41 0.17 0 0.08 0.1 0.14 0.18 0.09 0.06 0.09 0.11 0.11 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Neck 0 0 0 1 0 0 1 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 6 4 6 3 2 2 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 6 17 16 15 15 19 14 9 5 6 2 20 21 7 5 3 0 2 0 0 1 0
Endoplasmic Reticulum 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 4 1 3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 1 2 1 1 1 2 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Mitochondria 0 8 1 4 1 5 4 2 19 25 10 18 0 1 0 8 3 1 0 2 1 0 0 1
Nucleus 85 60 227 487 259 175 234 209 141 221 59 85 36 160 145 135 134 141 91 72 215 60 121 93
Nuclear Periphery 0 0 1 0 0 1 3 1 1 1 0 1 1 0 4 2 1 4 0 0 0 0 0 0
Nucleolus 22 15 22 59 15 20 38 38 78 88 45 52 8 7 14 15 21 29 10 5 24 8 18 24
Peroxisomes 2 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 2 0 0 0 1 3 0 0 0 1 2 3 5 1 1 2 1 0 1 0 0 3
Vac/Vac Membrane 0 1 2 2 2 4 2 5 1 1 0 0 3 2 4 3 3 0 1 3 6 2 10 10
Unique Cell Count 89 70 245 539 286 203 280 257 193 281 86 126 46 186 178 149 150 162 110 91 257 77 161 140
Labelled Cell Count 111 90 261 572 293 220 305 283 262 352 121 165 53 195 195 176 171 184 110 91 257 77 161 140


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 6.2 8.9 8.4 9.5 7.5 7.1 7.2 6.3 6.5 5.9 5.8 7.6 8.1 7.8 6.6 8.5 8.4 9.4 9.6 8.8
Std Deviation (1e-4) 1.7 1.2 1.6 1.8 3.2 1.6 1.7 1.8 1.9 1.6 1.6 1.2 1.9 2.3 2.3 1.4 2.2 2.1 2.0 2.0 1.9
Intensity Change (Log2) – – – -0.09 0.09 -0.26 -0.33 -0.31 -0.51 -0.47 -0.59 -0.63 -0.23 -0.13 -0.19 -0.44 -0.07 -0.1 0.08 0.1 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 1.8 2.5 1.7 2.5 2.4 0.5 0 0 0 3.6 3.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.7 4.5 0 5.7 0 0 0 0 0 0
Nucleus -1.0 -0.9 -2.2 -3.2 -3.8 -5.6 -4.5 -5.6 -6.3 -3.0 -2.3 -3.5 -0.7 -1.1 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 -1.7 0.3 1.6 2.0 7.8 6.3 8.6 7.4 0 -2.1 -0.4 0.4 1.6 2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2278 2.9085 2.9698 2.1086 2.4898 2.773 6.0845 6.0837 6.1375 6.0522 5.6588 5.8158 3.2857 3.5777 3.2223 2.9003 2.6304 3.0867
Actin 0.036 0.0003 0.007 0 0.0016 0.0004 0.0089 0 0.0009 0 0.0241 0.0003 0.0013 0 0.0043 0.0035 0.035 0.0028
Bud 0.0005 0.0001 0.0005 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0006 0.0004 0.0006 0 0.0002 0.0002 0.0007 0.0001
Bud Neck 0.0012 0.0002 0.0015 0.0001 0.0007 0.0005 0.0045 0.0002 0.001 0.0001 0.0004 0.0008 0.0017 0.0001 0.0004 0.0003 0.0027 0.0025
Bud Periphery 0.0012 0.0006 0.0009 0 0.0003 0.0005 0.0008 0 0.0004 0 0.0012 0.001 0.0016 0 0.0004 0.0003 0.0006 0.0002
Bud Site 0.0024 0.0003 0.0027 0 0.0003 0.0002 0.0056 0.0001 0.0016 0 0.0012 0.0008 0.0014 0.0001 0.0014 0.0007 0.0077 0.0002
Cell Periphery 0.0006 0.0002 0.0002 0 0.0001 0.0002 0.0003 0 0.0002 0 0.0001 0.0003 0.0004 0 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0013 0 0.0013 0 0.0001 0.0003 0.0027 0 0.0002 0 0.0004 0.0007 0.0023 0 0.008 0.0002 0.0095 0.0004
Cytoplasmic Foci 0.0074 0.0003 0.0054 0 0.0192 0.0002 0.0104 0 0.0001 0 0.0021 0.0023 0.0051 0 0.0031 0.0083 0.0313 0.0004
Eisosomes 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0037 0.0001 0.0015 0 0 0.0007 0.0015 0 0.0002 0 0.0041 0.0004 0.0005 0 0.0004 0.0006 0.0014 0.0007
Endosome 0.0107 0.0002 0.0127 0 0.0007 0.0007 0.0082 0 0.0002 0 0.0091 0.0011 0.0023 0 0.007 0.0175 0.0385 0.0041
Golgi 0.0046 0.0002 0.0049 0 0.0009 0.0001 0.0035 0 0.0001 0 0.0063 0.0011 0.0017 0 0.0047 0.0121 0.0083 0.0086
Lipid Particles 0.0067 0.0043 0.0123 0 0.0053 0.0009 0.0084 0 0.0002 0 0.0015 0.0018 0.006 0 0.0056 0.0357 0.0099 0.0006
Mitochondria 0.0052 0.0058 0.008 0.0001 0.0011 0.0041 0.0036 0.0001 0.0014 0 0.0156 0.0007 0.0057 0.0003 0.0036 0.0394 0.0024 0.0028
None 0.0011 0.0002 0.001 0 0.0002 0.0002 0.006 0 0.0003 0 0.0006 0.0001 0.0008 0 0.0002 0.0002 0.0031 0.0001
Nuclear Periphery 0.0089 0.0072 0.0064 0.0002 0.0012 0.0102 0.0117 0.0003 0.0007 0.0001 0.0244 0.0002 0.0046 0.0001 0.0011 0.0023 0.0026 0.0023
Nucleolus 0.0406 0.0363 0.028 0.0102 0.1817 0.0802 0.0447 0.0252 0.0184 0.0165 0.0852 0.0773 0.0449 0.0251 0.0162 0.0197 0.0886 0.0738
Nucleus 0.846 0.9398 0.892 0.9893 0.7717 0.8972 0.8496 0.9729 0.9726 0.9831 0.8141 0.9058 0.8925 0.974 0.939 0.8478 0.7386 0.899
Peroxisomes 0.0015 0.0006 0.0037 0 0.011 0.0001 0.0041 0 0.0001 0 0.0022 0.0028 0.0086 0 0.0024 0.0045 0.0043 0.0001
Punctate Nuclear 0.0173 0.0018 0.0061 0.0001 0.0031 0.001 0.0223 0.001 0.0008 0.0001 0.0041 0.0005 0.0144 0.0001 0.0004 0.0011 0.0121 0.0003
Vacuole 0.002 0.0005 0.0023 0 0.0004 0.001 0.0018 0 0.0003 0 0.0008 0.0013 0.0026 0 0.0011 0.0029 0.002 0.0007
Vacuole Periphery 0.0011 0.001 0.0014 0 0.0002 0.0011 0.001 0 0.0001 0 0.0016 0.0001 0.001 0 0.0004 0.0025 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.707 26.5563 23.4594 28.532 28.4288 46.6567 31.3262 33.2248 44.3713 32.6404
Translational Efficiency 1.0841 0.9471 1.051 1.1002 1.0051 0.6182 0.7548 0.8854 0.6382 0.7614

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
37 596 216 1793 1656 1802 460 887 1693 2398 676 2680

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 887.60 1047.31 1230.64 1074.55 777.91 1040.84 1121.35 1048.51 780.31 1042.45 1156.27 1065.93
Standard Deviation 231.94 150.30 163.98 157.75 138.40 137.88 192.54 180.33 142.02 141.10 190.83 166.02
Intensity Change Log 2 — 0.238707 0.471427 0.275751 — 0.420073 0.527561 0.430665 — 0.326261 0.497918 0.350181

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001748 0.000591 0.001440 0.001228 0.000500 0.000387 0.001031 0.001137 0.000527 0.000438 0.001162 0.001198
Bud Neck 0.036900 0.010459 0.014057 0.046439 0.005734 0.011858 0.012424 0.022397 0.006415 0.011510 0.012946 0.038482
Bud Site 0.028866 0.000894 0.001664 0.008570 0.001162 0.001670 0.001163 0.002090 0.001767 0.001477 0.001323 0.006425
Cell Periphery 0.000370 0.000043 0.000047 0.000183 0.000293 0.000118 0.000114 0.000147 0.000295 0.000099 0.000092 0.000171
Cytoplasm 0.029162 0.000420 0.000214 0.005878 0.003332 0.000536 0.003581 0.004833 0.003896 0.000507 0.002505 0.005532
Cytoplasmic Foci 0.037896 0.000150 0.000065 0.003640 0.000322 0.000248 0.001156 0.003048 0.001144 0.000224 0.000807 0.003444
Eisosomes 0.000151 0.000056 0.000086 0.000038 0.000042 0.000065 0.000069 0.000043 0.000044 0.000063 0.000074 0.000040
Endoplasmic Reticulum 0.009630 0.002134 0.002704 0.003725 0.002991 0.003064 0.002431 0.003046 0.003136 0.002833 0.002518 0.003500
Endosome 0.040365 0.000070 0.000101 0.005253 0.000405 0.000942 0.000483 0.001092 0.001278 0.000725 0.000361 0.003876
Golgi 0.008885 0.000071 0.000151 0.004466 0.000480 0.000134 0.000135 0.001207 0.000664 0.000118 0.000140 0.003388
Lipid Particles 0.005926 0.000029 0.000085 0.000390 0.000052 0.000121 0.000495 0.000097 0.000180 0.000098 0.000364 0.000293
Mitochondria 0.012598 0.000763 0.001786 0.008092 0.000585 0.001936 0.001293 0.002219 0.000847 0.001644 0.001450 0.006148
Mitotic Spindle 0.007530 0.001949 0.001126 0.053340 0.000032 0.001700 0.000551 0.010313 0.000196 0.001762 0.000734 0.039099
None 0.005980 0.001122 0.001036 0.004958 0.011225 0.000788 0.013765 0.007033 0.011111 0.000871 0.009698 0.005645
Nuclear Periphery 0.000616 0.000063 0.000138 0.004019 0.000055 0.000246 0.000150 0.001170 0.000068 0.000201 0.000146 0.003076
Nuclear Periphery Foci 0.017675 0.000072 0.000088 0.001104 0.000231 0.000198 0.000132 0.000427 0.000612 0.000167 0.000118 0.000880
Nucleolus 0.054634 0.084488 0.055396 0.015333 0.056634 0.066612 0.046534 0.026463 0.056591 0.071055 0.049366 0.019017
Nucleus 0.622728* 0.894832* 0.915970* 0.817833* 0.914344* 0.906260* 0.911947* 0.911097* 0.907971* 0.903420* 0.913232* 0.848700*
Peroxisomes 0.015148 0.001023 0.002391 0.001487 0.000478 0.000461 0.001751 0.000551 0.000799 0.000601 0.001955 0.001177
Vacuole 0.054284 0.000220 0.001261 0.008809 0.000797 0.001613 0.000647 0.001237 0.001966 0.001267 0.000843 0.006303
Vacuole Periphery 0.008908 0.000552 0.000196 0.005218 0.000306 0.001043 0.000149 0.000353 0.000494 0.000921 0.000164 0.003607

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.40 -0.17 0.68 -12.74 2.06 2.74 -10.55 -15.06 -16.12 -1.80 2.34 -13.46 -18.35 -20.08 -0.68
Bud Neck 1.77 1.43 -0.72 -17.69 -14.98 -7.46 -8.34 -12.42 -8.48 -6.35 -6.72 -9.53 -23.72 -19.48 -16.11
Bud Site 2.20 2.10 1.74 -7.26 -5.60 -1.14 -0.91 -4.80 -2.58 -6.23 0.56 0.20 -5.51 -6.54 -6.92
Cell Periphery 2.76 2.63 1.91 -4.40 -3.94 1.35 1.50 1.23 -0.61 -1.32 1.55 1.67 1.27 -1.53 -2.34
Cytoplasm 2.05 2.05 1.75 -6.76 -7.05 3.31 0.45 -1.14 -3.50 -1.41 3.93 1.82 -1.05 -7.10 -3.37
Cytoplasmic Foci 2.88 2.88 2.78 -3.35 -3.72 0.36 -1.09 -1.49 -1.57 -0.81 2.51 0.96 -0.69 -3.04 -1.56
Eisosomes 4.05 2.33 4.72 3.01 10.21 -5.49 -6.92 -1.45 5.37 7.02 -4.84 -8.85 0.26 6.91 12.02
Endoplasmic Reticulum 3.24 2.60 2.22 -11.08 -2.03 -0.29 1.83 -1.83 -1.24 -3.95 1.11 1.11 -4.34 -5.69 -5.81
Endosome 2.70 2.70 2.58 -4.86 -4.62 -1.75 -0.05 -1.14 0.29 -0.94 1.45 2.20 -0.54 -2.83 -3.80
Golgi 2.71 2.67 2.16 -4.04 -3.74 0.92 0.90 -0.68 -1.64 -1.63 1.50 1.39 -1.79 -4.24 -4.13
Lipid Particles 2.10 2.06 2.01 -5.06 -2.34 -1.76 -1.42 -2.80 0.00 1.12 1.03 -0.82 -0.68 -2.67 0.59
Mitochondria 2.41 2.16 1.20 -8.18 -6.39 -3.28 -9.47 -8.88 -1.46 -4.57 -2.51 -6.18 -9.09 -6.14 -7.44
Mitotic Spindle 1.90 1.86 -4.48 -9.55 -9.63 -2.69 -1.77 -4.90 -4.29 -2.25 -2.39 -1.93 -11.91 -11.05 -7.93
None 2.27 2.27 0.47 -6.62 -7.62 7.29 0.01 2.86 -5.92 1.40 7.30 1.17 4.01 -9.88 1.18
Nuclear Periphery 2.75 2.04 -4.30 -5.63 -5.36 -4.32 -7.62 -3.28 -2.71 -2.96 -3.96 -7.41 -6.34 -5.96 -6.04
Nuclear Periphery Foci 2.64 2.63 2.55 -4.35 -3.71 -0.13 1.80 -1.90 -1.76 -2.24 2.29 2.82 -0.54 -3.52 -4.37
Nucleolus -2.40 -0.44 3.36 14.77 6.51 -3.42 1.43 9.72 12.51 4.91 -5.29 0.65 15.81 21.97 9.19
Nucleus -4.95 -5.07 -4.14 6.69 6.38 1.92 1.65 3.12 1.74 0.89 0.93 0.72 8.47 8.32 6.34
Peroxisomes 2.42 2.15 2.46 0.83 4.67 0.09 -6.33 -2.19 -1.67 5.83 1.31 -5.94 0.39 -0.48 5.39
Vacuole 4.06 3.91 3.54 -7.23 -4.27 -1.90 -0.05 -2.46 -0.99 -2.59 1.47 1.61 -4.60 -5.95 -6.14
Vacuole Periphery 2.01 2.10 1.60 -2.25 -3.22 -3.36 1.74 -0.12 3.34 -2.09 -1.98 2.52 -2.53 -1.43 -3.39
Standard name
Human Ortholog
Description Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sgf29

Sgf29


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgf29-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available