Standard name
Human Ortholog
Description Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0 0 0 0 0.15 0.12 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.97 0.99 0.9 0.86 0.86 0.86 0.84 0.78 0.77 0.97 0.98 0.98 0.97 0.98 0.99 0.97 0.99 0.97 0.93 0.94 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.09 0.09 0.08 0.08 0.08 0.09 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 2 3 13 19 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3 0 0 2 8 0 1 2 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 5 7 7 19 14 7 9 10 69 64 3 3 4 4 5 2 0 0 0 0 0 0
Cytoplasm 134 181 284 212 284 280 326 232 191 229 371 420 145 277 268 146 94 140 129 178 279 153 280 280
Endoplasmic Reticulum 1 1 3 6 1 22 34 24 18 23 40 51 1 1 1 7 2 6 0 0 1 1 1 1
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 1 4 1 0 0 0 0 0 1 0 0 0 0 1 1
Mitochondria 1 0 0 0 0 0 3 2 6 0 10 10 0 0 0 1 0 0 0 0 1 2 1 0
Nucleus 1 0 5 4 0 0 4 4 2 4 2 5 4 4 6 3 1 0 0 0 1 1 2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 0 1 0 5 18 11 6 13 26 24 0 5 2 1 1 1 1 0 0 0 3 3
Unique Cell Count 137 182 290 219 286 312 381 270 222 273 474 543 149 282 274 150 96 141 134 180 289 165 300 295
Labelled Cell Count 141 187 297 230 292 326 401 284 234 283 537 603 153 291 284 162 103 151 134 180 289 165 300 295


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.6 19.2 20.3 19.1 22.4 22.0 20.8 19.8 19.2 19.9 22.0 21.3 24.6 26.7 26.5 31.9 38.7 40.4 20.7 21.2 22.6
Std Deviation (1e-4) 4.6 3.8 3.9 3.9 4.4 4.7 4.4 4.9 4.9 4.9 5.6 5.6 6.1 6.2 7.2 12.3 14.1 14.8 6.1 6.5 6.6
Intensity Change (Log2) – – – -0.08 0.14 0.12 0.04 -0.03 -0.08 -0.03 0.12 0.07 0.28 0.4 0.39 0.66 0.93 1.0 0.03 0.06 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 0.6 2.7 1.5 0.7 1.6 1.4 5.8 5.0 0 0 0 0 0 0
Cytoplasm -0.8 1.4 -4.1 -5.5 -5.3 -5.2 -5.9 -7.5 -7.8 -0.4 0.3 -0.1 -0.4 0 1.0
Endoplasmic Reticulum 0 0 3.7 4.4 4.3 4.0 4.2 4.3 4.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.4 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.8 3.5 0 3.8 4.1 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.5621 19.9915 18.1968 17.4048 17.7631 18.9848 18.7016 21.6168 19.5075 18.9757 16.9745 19.251 19.7453 21.3291 20.4487 18.8253 18.0548 20.2761
Actin 0.0166 0.0032 0.0135 0.0016 0.003 0.0111 0.0669 0.0205 0.0198 0.0007 0.0371 0.0176 0.0233 0.0051 0.0105 0.008 0.0316 0.0114
Bud 0.0003 0.0001 0.0002 0.0001 0.0001 0.0003 0.0006 0.0001 0.0002 0 0.0014 0.0001 0.0004 0.0001 0.0002 0.0001 0.0026 0.0009
Bud Neck 0.0013 0.0006 0.0016 0.0005 0.0019 0.004 0.0014 0.0007 0.0014 0.0023 0.0034 0.0039 0.0027 0.0026 0.0021 0.0026 0.0027 0.0056
Bud Periphery 0.0004 0 0.0003 0 0 0.0009 0.0006 0 0.0002 0 0.0044 0.0001 0.0004 0.0002 0.0002 0.0002 0.0127 0.0009
Bud Site 0.0003 0.0005 0.0007 0 0.0001 0.0001 0.0016 0.0006 0.0009 0 0.0008 0.0002 0.0019 0.001 0.0011 0.0002 0.0006 0.0002
Cell Periphery 0.0014 0.0005 0.0038 0 0.0001 0.0031 0.0007 0.0004 0.0006 0.0001 0.0004 0.0002 0.0017 0.0029 0.0006 0.0011 0.0004 0.0005
Cytoplasm 0.9696 0.9904 0.9671 0.9936 0.9891 0.9716 0.8552 0.975 0.9365 0.9947 0.8199 0.9716 0.9201 0.9848 0.9714 0.9819 0.9045 0.9708
Cytoplasmic Foci 0.0015 0.0007 0.0016 0.0011 0.0006 0.0015 0.0106 0.0011 0.0052 0.0004 0.0158 0.0024 0.0102 0.0004 0.0041 0.0006 0.0016 0.0019
Eisosomes 0.0008 0 0.0012 0 0 0 0.0004 0 0.0002 0 0.0006 0 0.0007 0 0 0.0001 0.0003 0
Endoplasmic Reticulum 0.0037 0.0024 0.0028 0.0012 0.0024 0.0039 0.0058 0.0005 0.0014 0.0006 0.0056 0.0011 0.0034 0.0019 0.0013 0.0028 0.0015 0.0028
Endosome 0.0006 0.0002 0.0015 0.0006 0.0002 0.0006 0.0168 0.0001 0.007 0.0001 0.028 0.0003 0.0134 0 0.0031 0.0003 0.0032 0.0003
Golgi 0.0011 0.0002 0.0007 0.0002 0.0001 0.0006 0.0124 0.0004 0.0065 0 0.0338 0.0007 0.0044 0.0002 0.0011 0.0006 0.0056 0.001
Lipid Particles 0.0004 0 0.0005 0 0 0.0002 0.0019 0 0.0033 0 0.0066 0.0001 0.0041 0.0001 0.0004 0.0001 0.0032 0.0001
Mitochondria 0.0004 0 0.0014 0 0.0001 0.0002 0.0046 0 0.0081 0 0.014 0.0003 0.0035 0 0.0002 0.0002 0.0213 0.0011
None 0.0007 0.0003 0.0007 0.0002 0.0001 0.0004 0.0026 0.0002 0.0006 0.0003 0.0009 0.0004 0.0015 0.0003 0.0008 0.0004 0.0008 0.0012
Nuclear Periphery 0.0003 0.0002 0.0006 0.0001 0.0008 0.0004 0.0074 0 0.0002 0.0001 0.0013 0.0001 0.002 0.0001 0.0002 0.0002 0.001 0.0002
Nucleolus 0 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0.0003 0.0001
Nucleus 0.0004 0.0003 0.0008 0.0004 0.001 0.0006 0.0031 0.0002 0.0003 0.0006 0.0007 0.0004 0.0009 0.0002 0.0006 0.0004 0.0013 0.0005
Peroxisomes 0.0001 0 0.0001 0 0 0.0001 0.0041 0.0001 0.0065 0 0.0214 0.0002 0.0021 0 0.0003 0 0.0012 0.0001
Punctate Nuclear 0.0001 0 0.0004 0 0.0001 0.0002 0.0007 0 0.0002 0.0001 0.0005 0.0001 0.0011 0 0.0002 0 0.0003 0.0001
Vacuole 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0013 0 0.0007 0 0.0018 0 0.0013 0 0.0016 0.0001 0.0014 0.0002
Vacuole Periphery 0 0 0.0001 0 0 0 0.0012 0 0.0002 0 0.0015 0 0.0005 0 0.0001 0 0.0019 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 163.1378 139.2063 169.2105 162.4871 137.4849 155.6362 173.9285 180.0432 213.9369 139.7176
Translational Efficiency 1.5181 1.7809 1.594 1.915 1.8102 1.7393 1.6748 1.9335 1.5538 1.6943

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1865 779 2568 1448 1349 1847 2312 1944 3214 2626 4880 3392

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1571.12 1669.51 2275.24 2697.44 1648.31 1881.87 2550.82 2803.14 1603.52 1818.87 2405.80 2758.02
Standard Deviation 254.04 371.10 372.11 555.46 276.95 299.71 426.69 543.28 266.63 336.81 421.97 551.00
Intensity Change Log 2 — 0.087631 0.534225 0.779797 — 0.191179 0.629974 0.766056 — 0.141574 0.584041 0.772778

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.008738 0.017952 0.009494 0.205243* 0.008518 0.021713 0.140444* 0.166067* 0.008646 0.020598 0.071534 0.182791*
Bud Neck 0.002805 0.004850 0.005226 0.020276 0.002143 0.004857 0.017428 0.019909 0.002527 0.004855 0.011007 0.020066
Bud Site 0.040882 0.079063 0.049112 0.123415* 0.030808 0.059891 0.094487 0.111380* 0.036653 0.065579 0.070609 0.116518*
Cell Periphery 0.002724 0.004703 0.012016 0.080622 0.002122 0.003180 0.034114 0.131471* 0.002472 0.003632 0.022485 0.109765*
Cytoplasm 0.813036* 0.642275* 0.813900* 0.273506* 0.819871* 0.756767* 0.380949* 0.289913* 0.815904* 0.722803* 0.608780* 0.282909*
Cytoplasmic Foci 0.045981 0.069323 0.009325 0.010003 0.046826 0.061507 0.021413 0.013900 0.046336 0.063826 0.015052 0.012237
Eisosomes 0.000167 0.000119 0.000149 0.014286 0.000073 0.000566 0.003880 0.016700 0.000127 0.000434 0.001916 0.015669
Endoplasmic Reticulum 0.003931 0.004889 0.000414 0.001795 0.004723 0.001738 0.004795 0.002781 0.004264 0.002673 0.002490 0.002360
Endosome 0.001111 0.010493 0.000490 0.002642 0.001781 0.001774 0.006052 0.007721 0.001392 0.004360 0.003125 0.005553
Golgi 0.006003 0.025444 0.001601 0.005119 0.005550 0.003740 0.006290 0.008951 0.005813 0.010179 0.003823 0.007315
Lipid Particles 0.011149 0.056564 0.038022 0.164864* 0.008413 0.012529 0.201088* 0.152938* 0.010001 0.025592 0.115278* 0.158029*
Mitochondria 0.000582 0.000261 0.000215 0.000397 0.000171 0.000361 0.000599 0.000429 0.000410 0.000331 0.000397 0.000415
Mitotic Spindle 0.000547 0.004348 0.000286 0.003833 0.001541 0.001613 0.002057 0.003069 0.000964 0.002424 0.001125 0.003395
None 0.007433 0.002773 0.001926 0.000771 0.005428 0.005171 0.002029 0.000748 0.006591 0.004460 0.001975 0.000758
Nuclear Periphery 0.000033 0.000130 0.000059 0.000276 0.000056 0.000054 0.000192 0.000370 0.000043 0.000076 0.000122 0.000330
Nuclear Periphery Foci 0.005272 0.025246 0.002386 0.002923 0.008332 0.004027 0.007407 0.003881 0.006557 0.010322 0.004765 0.003472
Nucleolus 0.000178 0.000161 0.000100 0.000140 0.000097 0.000157 0.000094 0.000060 0.000144 0.000158 0.000097 0.000094
Nucleus 0.034135 0.015414 0.017938 0.011482 0.036129 0.036673 0.016261 0.008157 0.034972 0.030367 0.017143 0.009576
Peroxisomes 0.001111 0.001510 0.001019 0.002207 0.000520 0.002269 0.004150 0.003063 0.000863 0.002043 0.002502 0.002698
Vacuole 0.013505 0.032055 0.035948 0.075096 0.016486 0.021137 0.055862 0.057508 0.014757 0.024376 0.045383 0.065016
Vacuole Periphery 0.000677 0.002426 0.000373 0.001104 0.000410 0.000274 0.000409 0.000985 0.000565 0.000912 0.000390 0.001036

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.75 -1.21 -25.59* -22.54* -25.42* -6.24 -25.17 -28.22* -25.41* -5.06 -7.82 -23.32 -38.27* -34.01* -21.91*
Bud Neck -6.32 -8.52 -23.97 -19.47 -19.46 -8.03 -25.44 -23.90 -19.33 -2.57 -9.35 -25.28 -33.17 -26.93 -14.47
Bud Site -7.80 -3.56 -18.91 -6.98 -17.44 -9.01 -18.66 -20.89 -13.38 -4.82 -11.12 -16.11 -27.77 -15.68 -16.00
Cell Periphery -3.29 -11.01 -19.56 -18.79 -16.37 -2.57 -19.09 -27.43* -27.21* -20.15 -3.43 -21.68 -32.95 -32.55* -25.95
Cytoplasm 14.79 1.68 56.73 25.24 56.22* 8.82 49.84 59.98* 54.33 9.45 16.22 35.05 83.50* 59.61 43.28
Cytoplasmic Foci -6.65 20.54 17.20 16.92 -3.13 -5.18 10.50 14.21 20.89 5.70 -8.51 21.30 21.86 27.20 2.39
Eisosomes 1.83 1.12 -8.81 -8.85 -8.83 -3.35 -10.52 -12.15 -11.70 -9.39 -2.91 -10.23 -14.97 -14.56 -13.16
Endoplasmic Reticulum -0.99 7.12 4.30 4.85 -3.99 4.52 0.27 2.91 -3.00 3.28 3.60 4.30 4.72 1.36 0.41
Endosome -8.26 6.33 -3.34 6.82 -5.19 0.21 -9.07 -7.47 -7.36 -2.09 -7.20 -7.49 -8.42 -1.99 -4.66
Golgi -7.68 13.38 2.11 8.04 -2.95 2.89 -0.22 -2.75 -4.79 -2.69 -4.81 6.01 -1.23 2.98 -4.45
Lipid Particles -9.98 -12.35 -23.25 -12.86 -17.84 -3.33 -33.61* -26.83 -25.88 4.83 -9.33 -33.24 -35.34 -29.75 -8.54
Mitochondria 1.40 1.64 1.06 -0.89 -1.99 -4.99 -7.32 -5.41 -1.38 1.71 0.67 0.38 0.20 -1.37 -0.54
Mitotic Spindle -3.41 3.28 -3.69 0.34 -3.92 -0.09 -2.62 -2.28 -1.96 -0.90 -2.80 -2.42 -4.35 -1.59 -3.46
None 5.55 7.48 9.01 4.07 6.47 0.60 6.73 9.74 11.69 5.73 4.14 9.64 12.29 12.22 8.63
Nuclear Periphery -2.16 -7.54 -13.79 -3.10 -11.97 0.37 -16.83 -15.97 -13.21 -6.47 -1.96 -18.13 -21.20 -11.93 -13.12
Nuclear Periphery Foci -8.84 6.75 2.46 9.36 -3.77 6.94 -0.72 5.26 -0.79 5.53 -4.66 2.14 5.10 7.53 2.70
Nucleolus 0.45 6.48 0.76 0.45 -0.31 -5.65 0.85 3.26 8.49 2.80 -0.71 6.16 1.67 1.90 0.16
Nucleus 11.25 10.28 12.41 -0.79 3.92 -0.53 9.75 20.51 23.97 13.20 3.44 14.00 23.85 19.09 13.00
Peroxisomes -1.81 1.54 -3.44 -1.15 -4.60 -8.73 -11.55 -8.71 -2.03 1.83 -7.55 -9.28 -8.68 -1.87 -1.33
Vacuole -8.86 -20.86 -25.41 -17.00 -17.49 -3.47 -21.90 -18.97 -17.92 2.08 -10.06 -29.44 -32.09 -25.55 -11.65
Vacuole Periphery -2.43 1.68 -0.56 2.00 -1.69 1.30 0.23 -1.43 -1.87 -1.54 -1.34 1.63 -1.31 -0.17 -2.21
Standard name
Human Ortholog
Description Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ycp4

Ycp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available