Standard name
Human Ortholog
Description Putative ATP-dependent permease of the ABC transporter family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0.1 0.12 0.08 0.06 0 0.09 – 0.07 0.06 0.05 0 0 0 0.12 0.13 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.06 0 – 0.08 0.08 0.08 0 0 0.07 0.17 0.11 0.12 0 0 0 0 0 0
Cytoplasm 0.23 0.28 0.15 0.24 0.22 0.27 0.27 0.3 – 0.44 0.48 0.42 0.23 0.26 0.4 0.61 0.62 0.57 0 0 0 0 0 0
Endoplasmic Reticulum 0.59 0.73 0.78 0.63 0.61 0.62 0.59 0.51 – 0.36 0.24 0.41 0.71 0.73 0.64 0 0.06 0 0.89 0.87 0.9 0.82 0.78 0.68
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0.05 0.08 0.07 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.07 0.09 – 0.16 0.26 0.15 0 0 0 0.07 0.11 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0 0.05 0 0.14 0.09 0.12 0.1 – 0 0 0 0.06 0.08 0 0.09 0.08 0.11 0 0 0 0.09 0.09 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 21 3 29 29 19 21 18 41 – 30 17 20 5 5 6 23 28 19 2 2 4 0 1 1
Bud 4 0 1 3 1 1 4 22 – 19 11 11 1 0 1 3 4 3 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 0 0 2
Bud Site 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 5 7 10 18 23 17 – 33 23 32 1 2 13 33 23 21 0 0 0 0 0 0
Cytoplasm 79 39 43 56 52 92 109 143 – 182 143 169 33 50 74 120 131 100 6 2 3 2 4 5
Endoplasmic Reticulum 201 102 229 151 145 212 238 238 – 150 72 164 102 140 119 6 13 6 347 137 275 177 177 190
Endosome 9 0 10 10 10 2 5 10 – 3 3 3 1 2 2 3 2 5 5 2 2 2 10 14
Golgi 9 1 15 18 16 4 8 4 – 1 0 0 0 1 0 0 1 12 2 1 3 3 2 4
Mitochondria 1 1 1 10 8 21 29 41 – 68 79 61 0 0 0 14 23 8 3 2 3 6 2 4
Nucleus 0 0 2 1 0 2 0 1 – 2 3 0 1 1 1 5 2 2 0 1 0 0 0 0
Nuclear Periphery 0 0 4 2 0 0 2 8 – 2 1 6 1 0 1 1 3 0 2 1 1 1 2 2
Nucleolus 3 0 0 2 1 1 0 1 – 3 0 2 0 1 1 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0
Vac/Vac Membrane 65 5 15 10 32 30 50 46 – 17 9 17 9 16 6 18 16 19 13 4 4 19 19 46
Unique Cell Count 339 140 294 238 236 340 404 471 416 300 399 143 192 186 197 212 174 390 158 304 217 226 281
Labelled Cell Count 394 152 354 299 294 404 486 572 511 361 485 154 218 224 226 249 195 390 158 304 217 226 281


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.3 12.5 12.1 12.2 11.7 10.8 10.8 10.4 – 9.8 9.7 10.0 12.1 12.0 12.8 9.2 10.7 13.5 12.8 12.7 13.2
Std Deviation (1e-4) 2.1 2.4 1.9 2.2 2.5 1.9 1.9 1.8 – 2.1 1.9 2.0 1.9 1.8 2.4 1.7 2.2 2.6 1.8 2.0 2.1
Intensity Change (Log2) – – – 0.01 -0.05 -0.17 -0.16 -0.22 – -0.3 -0.31 -0.27 0.0 -0.02 0.08 -0.39 -0.18 0.15 0.08 0.07 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 -0.7 -1.7 -2.8 -0.5 – -1.3 -1.9 -2.5 -2.3 -3.1 -2.7 0.6 1.2 0.4
Bud 0 0 0 0 3.4 – 3.4 2.9 2.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 1.8 2.4 2.7 1.5 – 3.6 3.4 3.7 0 0 3.0 6.1 4.4 4.7
Cytoplasm 2.6 2.2 3.8 3.9 4.9 – 8.2 8.7 7.8 2.2 3.1 6.3 10.7 11.0 9.7
Endoplasmic Reticulum -3.7 -4.1 -4.2 -5.3 -7.5 – -11.0 -13.1 -9.7 -1.5 -1.3 -3.3 -16.3 -15.9 -15.6
Endosome 0.5 0.5 -2.6 -1.9 -1.1 – -2.6 -2.0 -2.5 0 0 0 -1.3 -1.8 -0.3
Golgi 1.2 0.8 -2.9 -2.3 -3.7 – -4.3 -4.0 -4.6 0 -2.8 -3.1 -3.2 -2.9 0.8
Mitochondria 0 0 4.0 4.4 4.9 – 7.1 9.3 6.8 0 0 0 4.3 5.5 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -0.5 3.4 1.8 3.3 2.3 – -0.6 -1.3 -0.5 0.5 1.4 -1.0 1.8 1.1 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0367 0.0002 0.0022 0.0318 0.0183 0.0004 0.0258 0.0002 0.0026 0.0001 0.0005 0.0062 0.3376 0 0.0504 0.0001 0.0002 0.0001
Bud 0.0006 0.0001 0.0007 0.0004 0.0004 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0001 0.0023 0 0.0003 0.0001 0.0001 0
Bud Neck 0.0054 0.0004 0.0042 0.0007 0.001 0.0011 0.0004 0.0004 0.0003 0.0005 0.0005 0.0002 0.0033 0.0001 0.0003 0.0002 0.0004 0.0002
Bud Periphery 0.001 0.0003 0.0011 0.0007 0.0006 0.0003 0.0009 0.0001 0.0004 0.0003 0.0007 0.0002 0.0022 0 0.0005 0.0004 0.0004 0.0001
Bud Site 0.0076 0.0004 0.0107 0.0012 0.0018 0.0003 0.0009 0.0005 0.0002 0.0001 0.0001 0.0001 0.0058 0 0.0008 0.0001 0.0002 0
Cell Periphery 0.0018 0.0061 0.0031 0.0035 0.0018 0.0006 0.0035 0.0035 0.002 0.0029 0.0013 0.0007 0.0006 0.0023 0.0032 0.0008 0.0009 0.0004
Cytoplasm 0.0359 0.1342 0.0905 0.097 0.0917 0.0558 0.0173 0.0397 0.0317 0.0084 0.0129 0.0276 0.0034 0.0137 0.0273 0.0146 0.0146 0.0321
Cytoplasmic Foci 0.0337 0.0056 0.0115 0.0111 0.0248 0.004 0.0173 0.0032 0.008 0.0013 0.0036 0.0021 0.0424 0.0003 0.007 0.0017 0.0034 0.0011
Eisosomes 0.0004 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0014 0 0 0 0 0
Endoplasmic Reticulum 0.5559 0.5311 0.6189 0.4761 0.4778 0.6431 0.6519 0.8158 0.7798 0.7685 0.5386 0.7579 0.0076 0.7888 0.696 0.5101 0.6879 0.7973
Endosome 0.0721 0.0395 0.0418 0.0595 0.1015 0.0455 0.0935 0.0334 0.0324 0.0405 0.0825 0.0276 0.1581 0.0083 0.0684 0.075 0.1089 0.0399
Golgi 0.0129 0.0014 0.008 0.0051 0.0086 0.0019 0.0189 0.0026 0.004 0.0028 0.0107 0.0036 0.3339 0.0001 0.0044 0.0014 0.006 0.0008
Lipid Particles 0.0309 0.026 0.0098 0.0133 0.0131 0.0035 0.0342 0.0456 0.0065 0.0184 0.0108 0.0098 0.0299 0.0025 0.037 0.0036 0.0098 0.0019
Mitochondria 0.0053 0.0005 0.0163 0.001 0.0017 0.0009 0.0093 0.001 0.024 0.0007 0.0903 0.0007 0.0516 0.0001 0.0009 0.0013 0.0025 0.0007
None 0.0132 0.0004 0.0009 0.01 0.001 0.0003 0.0132 0.0002 0.0127 0.0001 0.0002 0.0004 0.0012 0 0.0003 0.0002 0.0002 0.0002
Nuclear Periphery 0.0776 0.0434 0.0405 0.0441 0.0682 0.1081 0.0316 0.0159 0.027 0.0221 0.0528 0.0631 0.0027 0.021 0.0168 0.0999 0.0542 0.046
Nucleolus 0.0021 0.0001 0.0004 0.0003 0.0008 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0 0 0
Nucleus 0.026 0.0042 0.0031 0.0048 0.0057 0.0087 0.0023 0.0009 0.0013 0.0009 0.0014 0.0019 0.0005 0.0012 0.0008 0.003 0.0016 0.0014
Peroxisomes 0.0082 0.0001 0.0045 0.0153 0.014 0.0001 0.0065 0 0.0009 0 0.0001 0.0014 0.0072 0 0.0035 0 0.0001 0
Punctate Nuclear 0.0066 0.0003 0.0005 0.0015 0.0014 0.0006 0.0025 0.0003 0.0024 0.0001 0.0001 0.0006 0.0012 0 0.0005 0.0002 0.0002 0.0001
Vacuole 0.0585 0.1912 0.1089 0.2006 0.1527 0.1045 0.0626 0.0333 0.0587 0.1245 0.1316 0.0841 0.0029 0.154 0.0766 0.2367 0.0925 0.0658
Vacuole Periphery 0.0076 0.0143 0.0221 0.0221 0.0132 0.0201 0.0066 0.0031 0.005 0.0076 0.0611 0.0115 0.0038 0.0073 0.0051 0.0506 0.0158 0.012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.1304 41.647 44.3807 59.6046 39.058 35.7095 64.7469 69.7701 66.425 63.2251
Translational Efficiency 0.8185 0.7484 0.7799 0.628 0.716 0.9506 0.7789 0.5178 0.6072 0.5844

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
940 889 224 101 1597 1129 939 1260 2537 2018 1163 1361

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1065.84 1185.93 1428.83 1396.90 1117.45 1058.99 1249.86 1186.33 1098.33 1114.91 1284.33 1201.96
Standard Deviation 131.08 201.48 178.84 185.20 198.33 228.30 161.19 187.30 178.18 225.86 179.22 195.12
Intensity Change Log 2 — 0.154028 0.422843 0.390238 — -0.077521 0.161556 0.086295 — 0.040160 0.295024 0.242672

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000544 0.000565 0.000142 0.000300 0.002832 0.000420 0.000224 0.000231 0.001984 0.000484 0.000208 0.000236
Bud Neck 0.000733 0.000940 0.003785 0.005702 0.000373 0.001466 0.002615 0.002790 0.000506 0.001234 0.002840 0.003006
Bud Site 0.002389 0.005272 0.002299 0.009540 0.007723 0.005357 0.003291 0.007207 0.005747 0.005320 0.003100 0.007380
Cell Periphery 0.000512 0.000897 0.000699 0.000856 0.011050 0.001037 0.000283 0.000610 0.007145 0.000975 0.000363 0.000629
Cytoplasm 0.018276 0.023992 0.012297 0.027230 0.025333 0.027223 0.041173 0.031757 0.022718 0.025800 0.035611 0.031421
Cytoplasmic Foci 0.008384 0.025466 0.004572 0.008926 0.022428 0.045007 0.015682 0.012111 0.017225 0.036399 0.013543 0.011875
Eisosomes 0.000084 0.000036 0.000009 0.000024 0.000642 0.000068 0.000035 0.000021 0.000435 0.000054 0.000030 0.000022
Endoplasmic Reticulum 0.838922* 0.767464* 0.364614* 0.375163* 0.874814* 0.754441* 0.582423* 0.569081* 0.861515* 0.760178* 0.540472* 0.554691*
Endosome 0.022205 0.041330 0.053608 0.065349 0.007013 0.042321 0.036442 0.062149 0.012642 0.041884 0.039748 0.062387
Golgi 0.006724 0.012272 0.001819 0.001776 0.006474 0.020755 0.002076 0.003906 0.006567 0.017018 0.002026 0.003748
Lipid Particles 0.017075 0.014365 0.012364 0.014946 0.017992 0.023306 0.020975 0.014158 0.017652 0.019367 0.019317 0.014217
Mitochondria 0.004414 0.001232 0.000148 0.000170 0.004391 0.004062 0.001101 0.000852 0.004400 0.002815 0.000918 0.000802
Mitotic Spindle 0.000616 0.001958 0.000301 0.002894 0.000065 0.000549 0.001252 0.001774 0.000269 0.001170 0.001069 0.001857
None 0.001134 0.001851 0.000227 0.000316 0.002484 0.002100 0.000501 0.001944 0.001984 0.001990 0.000448 0.001823
Nuclear Periphery 0.006605 0.002366 0.030985 0.007562 0.000083 0.003821 0.060511 0.016325 0.002499 0.003180 0.054824 0.015675
Nuclear Periphery Foci 0.020525 0.027804 0.122174 0.097345 0.000542 0.013489 0.085038 0.056350 0.007946 0.019795 0.092190 0.059393
Nucleolus 0.000052 0.000386 0.000153 0.000279 0.000131 0.000914 0.001244 0.000637 0.000102 0.000681 0.001034 0.000610
Nucleus 0.001048 0.000994 0.002442 0.002575 0.000554 0.003967 0.020385 0.005616 0.000737 0.002657 0.016929 0.005391
Peroxisomes 0.000345 0.000578 0.000252 0.000138 0.011746 0.001452 0.000631 0.000278 0.007522 0.001067 0.000558 0.000267
Vacuole 0.045841 0.054978 0.366435* 0.366329* 0.002045 0.032469 0.119457 0.199664 0.018272 0.042385 0.167026 0.212032*
Vacuole Periphery 0.003570 0.015253 0.020676 0.012581 0.001285 0.015776 0.004660 0.012537 0.002132 0.015546 0.007745 0.012541

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.17 3.35 1.36 1.13 -1.97 3.88 4.19 4.18 2.84 -0.14 3.70 4.50 4.41 2.56 -0.71
Bud Neck -0.95 -3.98 -4.56 -4.37 -1.77 -2.41 -7.29 -4.36 -1.90 -0.29 -2.69 -8.10 -4.87 -3.15 -0.37
Bud Site -2.49 0.07 -3.01 -1.84 -2.89 1.62 3.16 0.24 -1.22 -2.61 0.35 2.51 -1.27 -1.58 -3.16
Cell Periphery -1.53 -1.19 -1.74 0.14 -0.76 9.00 9.87 9.55 1.95 -4.74 8.65 9.75 9.34 2.09 -3.90
Cytoplasm -2.03 1.49 -1.62 -0.84 -2.24 -0.28 -3.96 -1.81 -1.48 2.14 -1.15 -4.02 -3.02 -2.13 0.98
Cytoplasmic Foci -6.19 2.40 -0.30 5.55 -2.28 -6.00 2.70 4.89 9.59 2.13 -7.71 1.97 3.43 10.48 1.20
Eisosomes 1.92 3.11 2.45 1.39 -3.64 7.84 8.32 8.52 5.99 4.13 8.06 8.60 8.80 5.74 3.05
Endoplasmic Reticulum 5.20 20.58 13.62 11.47 0.12 10.32 22.79 26.47 13.03 0.92 11.54 27.77 28.13 17.00 -0.82
Endosome -6.28 -6.26 -6.11 -3.27 -1.31 -14.79 -13.87 -21.35 -5.74 -7.74 -15.84 -13.17 -19.64 -6.97 -7.26
Golgi -2.82 3.56 3.66 6.98 0.18 -7.05 5.69 3.30 8.37 -2.60 -7.43 6.10 3.61 9.78 -2.79
Lipid Particles 0.94 1.29 0.20 -0.52 -0.86 -2.10 -1.35 1.89 3.66 3.04 -0.89 -0.88 1.87 2.62 2.52
Mitochondria 1.74 2.36 2.34 4.52 -0.52 0.28 3.92 4.46 4.05 0.67 1.66 4.00 4.26 4.29 0.35
Mitotic Spindle -1.73 1.01 -1.27 -0.97 -1.33 -3.30 -2.88 -3.32 -2.38 -0.93 -2.54 -2.31 -3.14 -1.49 -1.67
None -0.60 0.92 0.78 1.96 -0.33 0.42 2.98 0.50 0.15 -1.56 -0.01 2.74 0.16 0.18 -1.62
Nuclear Periphery 3.70 -5.81 -0.40 -2.93 5.37 -4.35 -14.24 -9.94 -6.75 9.70 -1.06 -14.73 -8.36 -7.79 10.18
Nuclear Periphery Foci -2.10 -8.38 -4.16 -3.74 1.22 -9.73 -17.57 -16.35 -11.79 4.70 -7.25 -18.32 -14.60 -10.70 5.63
Nucleolus -1.53 -2.30 -2.22 0.52 -1.28 -3.03 -3.41 -2.08 0.98 1.67 -3.28 -3.61 -2.42 0.43 1.37
Nucleus -0.05 -0.80 -1.84 -1.80 -0.25 -3.64 -8.57 -5.46 -1.08 5.95 -3.60 -8.49 -5.47 -2.58 5.52
Peroxisomes -1.22 0.39 1.94 2.36 0.52 8.45 9.28 9.60 5.04 3.60 8.28 9.11 9.52 5.02 3.12
Vacuole -1.32 -15.21 -10.77 -10.47 -0.22 -12.54 -19.05 -27.76 -22.39 -8.46 -7.90 -20.80 -26.79 -22.79 -4.61
Vacuole Periphery -5.78 -4.95 -2.59 0.84 1.81 -9.09 -5.79 -11.50 1.76 -7.08 -10.77 -6.45 -10.60 2.03 -3.90
Standard name
Human Ortholog
Description Putative ATP-dependent permease of the ABC transporter family
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Adp1

Adp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available