Standard name
Human Ortholog
Description Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.96 0.97 0.95 0.96 0.89 0.94 – 0.78 0.83 0.74 0.8 0.99 1.0 0.99 – 0.93 0.94 0.64 0.81 0.65 0.78 0.75 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.1 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.06 0 0.05 0 0.23 0 – 0.33 0.25 0.33 0.29 0 0 0 – 0.1 0 0.15 0.08 0.18 0.08 0.09 0.07
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 – 1 0 7 2 2 2 5 7
Bud 0 1 1 0 0 2 1 – 1 3 3 3 0 0 0 – 0 0 2 0 0 1 7 5
Bud Neck 1 0 2 0 2 0 4 – 3 4 5 2 1 0 0 – 0 0 1 2 0 1 1 3
Bud Site 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 1 0 0 0 – 0 2 1 0 1 0 1 – 2 2 0 1 0 1 3 3
Cytoplasm 177 250 76 58 82 96 171 – 99 200 144 163 225 352 396 – 54 96 133 207 62 134 222 277
Endoplasmic Reticulum 1 2 0 0 0 0 0 – 0 1 1 0 1 0 0 – 6 5 2 1 1 0 0 1
Endosome 4 0 0 0 2 0 1 – 0 0 0 0 2 0 0 – 1 3 7 2 0 1 2 2
Golgi 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 1 5 0 0 3 5 0
Mitochondria 11 15 1 3 0 25 7 – 42 60 65 60 1 0 2 – 6 2 31 21 17 13 26 22
Nucleus 0 0 0 0 1 2 0 – 0 1 5 1 0 0 1 – 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 – 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 1 0 1 0 – 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 2 0 0 1 0
SpindlePole 1 0 0 0 0 1 4 – 0 2 5 5 0 0 0 – 0 0 5 1 0 2 8 5
Vac/Vac Membrane 7 0 0 0 1 0 2 – 1 1 2 2 0 0 0 – 0 0 5 4 2 1 4 4
Unique Cell Count 198 261 78 61 85 108 181 127 240 195 205 228 353 398 58 102 210 256 95 172 297 343
Labelled Cell Count 206 268 81 64 88 127 190 147 274 233 236 231 353 400 71 111 210 256 95 172 297 343


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.6 4.2 4.1 4.9 3.2 4.3 – 3.4 3.3 3.5 3.5 4.2 4.5 4.8 4.7 5.1 5.0 4.1 4.1 4.0
Std Deviation (1e-4) 1.4 1.0 0.9 1.3 1.2 0.9 2.3 – 1.4 0.9 1.1 1.3 1.0 1.4 1.6 0.8 1.6 1.1 2.4 1.9 1.5
Intensity Change (Log2) – – – -0.04 0.22 -0.38 0.05 – -0.31 -0.33 -0.25 -0.24 0.01 0.09 0.2 0.17 0.28 0.26 -0.02 -0.04 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.7259 -1.3374 -1.4974 -1.7101 -1.6346 -1.51 0.6408 -0.1817 -0.1608 -0.5441 0.4466 -0.3621 1.5727 2.5733 2.1853 1.9673 2.1912 2.1308
Actin 0.0014 0.0008 0.0063 0.0002 0.02 0.002 0.049 0.0047 0.0174 0.0001 0.0022 0.005 0.0003 0.0003 0.0003 0.032 0.0017 0.0002
Bud 0.0003 0.0055 0.001 0 0.0013 0.0001 0.001 0.0011 0.0013 0 0.0256 0.0002 0.002 0.0017 0.0018 0.0001 0.0127 0.0001
Bud Neck 0.001 0.0008 0.0008 0 0.0012 0.0003 0.0013 0.0003 0.0111 0 0.0021 0.0004 0.0006 0.0004 0.0002 0.0003 0.0003 0.0001
Bud Periphery 0.0003 0.0018 0.0007 0.0001 0.0029 0.0001 0.0012 0.0011 0.001 0 0.0429 0.0004 0.0025 0.0013 0.0012 0.0001 0.0062 0
Bud Site 0.0017 0.0114 0.0047 0 0.0077 0 0.0239 0.01 0.0058 0 0.0253 0.0001 0.0036 0.009 0.0015 0.0006 0.0009 0
Cell Periphery 0.0001 0.0004 0.0002 0 0.0002 0 0.0003 0.0002 0.0001 0 0.0019 0.0001 0.0004 0.0005 0.0002 0 0.0002 0
Cytoplasm 0.0655 0.1049 0.0467 0.0984 0.0929 0.098 0.0699 0.1038 0.12 0.0394 0.0094 0.1311 0.0293 0.0801 0.0637 0.0801 0.0404 0.1114
Cytoplasmic Foci 0.0214 0.0146 0.0298 0.0125 0.0061 0.0123 0.0394 0.0092 0.0218 0.0015 0.0056 0.0119 0.0019 0.0024 0.0021 0.0018 0.0052 0.0016
Eisosomes 0.0007 0.0003 0.0002 0.0001 0.0002 0.0006 0.0004 0.0002 0.0003 0.0001 0 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0011 0.0006 0.0009 0.0007 0.0067 0.0005 0.0034 0.0005 0.0015 0.0002 0.0004 0.0006 0.0007 0.0006 0.0005 0.0002 0.0007 0.0005
Endosome 0.0102 0.0025 0.0091 0.0067 0.0037 0.0013 0.0396 0.0017 0.0131 0.0002 0.0153 0.0017 0.0016 0.0033 0.0003 0.0003 0.0114 0.0004
Golgi 0.002 0.0009 0.0018 0.0001 0.0014 0.0002 0.0201 0.0005 0.0134 0 0.0056 0.0034 0.0003 0.0002 0 0.0009 0.0013 0
Lipid Particles 0.0086 0.0035 0.0027 0.0003 0.0059 0.0003 0.0254 0.0005 0.0055 0.0001 0.0014 0.0017 0.0009 0.0003 0.0001 0.0003 0.0039 0
Mitochondria 0.0012 0.002 0.0019 0.0002 0.0034 0.0002 0.0078 0.0004 0.0214 0 0.0175 0.0229 0.0008 0.0026 0.0002 0.0005 0.0004 0.0001
None 0.8655 0.8234 0.8739 0.8759 0.8024 0.8719 0.6501 0.859 0.7329 0.9579 0.7913 0.8185 0.9359 0.8457 0.8901 0.8803 0.8762 0.8819
Nuclear Periphery 0.0022 0.0007 0.0014 0.0009 0.0012 0.0009 0.0061 0.0002 0.001 0.0001 0.0005 0.0002 0.0008 0.0005 0.0003 0.0001 0.0046 0.0003
Nucleolus 0.001 0.0115 0.0008 0.0001 0.0002 0.0006 0.001 0.0003 0.0001 0 0.0027 0 0.0024 0.0005 0.0009 0 0.0008 0.0001
Nucleus 0.0023 0.0048 0.0019 0.001 0.005 0.0032 0.016 0.0005 0.0143 0.0001 0.0024 0.0002 0.0117 0.0385 0.0341 0.0004 0.02 0.0027
Peroxisomes 0.0072 0.0054 0.0056 0.0002 0.0345 0.0005 0.0262 0.0035 0.0159 0 0.0031 0.0003 0.0002 0.0001 0.0001 0.0016 0.001 0
Punctate Nuclear 0.0051 0.0029 0.0034 0.0013 0.002 0.0065 0.0114 0.0019 0.0007 0.0001 0.0002 0.0003 0.0004 0.0011 0.0012 0.0002 0.0099 0.0003
Vacuole 0.001 0.0012 0.0053 0.0012 0.0009 0.0004 0.0047 0.0005 0.0009 0.0001 0.0371 0.0002 0.0027 0.0081 0.001 0.0001 0.0019 0.0002
Vacuole Periphery 0.0003 0.0002 0.0009 0.0002 0.0002 0.0001 0.0017 0.0001 0.0005 0 0.0073 0.0003 0.0004 0.0027 0.0001 0 0.0004 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.7028 3.0444 7.9067 4.8954 4.4155 10.2111 6.5411 5.1939 6.7779 6.9707
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
145 1454 189 1594 1564 1724 164 571 1709 3178 353 2165

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 631.69 733.05 3512.54 814.68 648.09 729.25 917.39 885.27 646.70 730.99 2306.86 833.30
Standard Deviation 78.44 88.95 1151.92 137.20 73.68 102.50 204.67 110.63 74.23 96.55 1550.86 134.37
Intensity Change Log 2 — 0.214695 2.475226 0.367017 — 0.170219 0.501341 0.449923 — 0.192343 1.791388 0.409596

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000505 0.000405 0.000354 0.000417 0.000225 0.000451 0.000484 0.000848 0.000249 0.000430 0.000414 0.000531
Bud Neck 0.057290 0.038488 0.003281 0.003778 0.019597 0.048455 0.001881 0.002388 0.022795 0.043895 0.002630 0.003411
Bud Site 0.028502 0.021012 0.002721 0.023850 0.006141 0.022612 0.004747 0.011001 0.008038 0.021880 0.003662 0.020461
Cell Periphery 0.001215 0.000150 0.001730 0.000180 0.000588 0.000288 0.000243 0.000136 0.000641 0.000225 0.001039 0.000168
Cytoplasm 0.370625* 0.382030* 0.432399* 0.614074* 0.475616* 0.452517* 0.491944* 0.514101* 0.466708* 0.420268* 0.460063* 0.587707*
Cytoplasmic Foci 0.260659* 0.291471* 0.034554 0.021182 0.256993* 0.206885* 0.025726 0.038750 0.257304* 0.245585* 0.030453 0.025815
Eisosomes 0.000863 0.000151 0.000006 0.000033 0.000380 0.000180 0.000079 0.000051 0.000421 0.000167 0.000040 0.000038
Endoplasmic Reticulum 0.001897 0.000888 0.000204 0.001422 0.000480 0.000880 0.004073 0.001671 0.000600 0.000884 0.002002 0.001488
Endosome 0.015427 0.026182 0.000327 0.003345 0.012264 0.013797 0.005064 0.004459 0.012533 0.019464 0.002528 0.003639
Golgi 0.004799 0.009101 0.000959 0.001355 0.003264 0.006728 0.000198 0.002136 0.003394 0.007813 0.000606 0.001561
Lipid Particles 0.020894 0.007321 0.001915 0.000918 0.010345 0.005257 0.003004 0.002205 0.011240 0.006202 0.002421 0.001257
Mitochondria 0.010889 0.019741 0.000362 0.000990 0.008852 0.012225 0.000250 0.000442 0.009025 0.015664 0.000310 0.000845
Mitotic Spindle 0.003176 0.004851 0.002694 0.021807 0.001075 0.005057 0.001490 0.004280 0.001253 0.004963 0.002135 0.017184
None 0.011818 0.003590 0.007600 0.008188 0.003181 0.004893 0.006688 0.003627 0.003914 0.004297 0.007176 0.006985
Nuclear Periphery 0.001689 0.000343 0.000816 0.000856 0.000673 0.000299 0.000993 0.001181 0.000759 0.000319 0.000898 0.000942
Nuclear Periphery Foci 0.002055 0.000427 0.001991 0.001812 0.000217 0.000161 0.005571 0.001493 0.000373 0.000283 0.003655 0.001728
Nucleolus 0.005857 0.002092 0.000136 0.000442 0.000871 0.001316 0.000611 0.000412 0.001294 0.001671 0.000357 0.000434
Nucleus 0.151473* 0.095262 0.484743* 0.191408* 0.154258* 0.151728* 0.320244* 0.288506* 0.154022* 0.125894 0.408318* 0.217017*
Peroxisomes 0.026017 0.018931 0.000033 0.000533 0.007827 0.014302 0.000402 0.001256 0.009370 0.016420 0.000204 0.000723
Vacuole 0.022925 0.074448 0.022980 0.101196 0.036138 0.049866 0.126079 0.120746 0.035017 0.061112 0.070879 0.106352
Vacuole Periphery 0.001425 0.003114 0.000196 0.002215 0.001015 0.002104 0.000227 0.000311 0.001050 0.002566 0.000210 0.001713

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.66 -1.85 1.19 1.10 4.33 -2.37 -3.89 -1.13 -0.35 0.12 -2.59 -5.73 -1.28 0.49 3.04
Bud Neck 1.52 4.11 4.46 14.99 5.04 -10.37 12.63 11.96 18.33 -1.70 -9.06 10.51 11.89 23.55 3.50
Bud Site 0.95 2.87 1.68 2.49 -4.61 -8.35 0.75 -0.94 5.03 -1.38 -8.46 1.94 -2.75 4.58 -4.92
Cell Periphery 2.96 -3.75 3.02 1.27 10.13 0.80 0.95 1.26 3.06 4.28 1.21 -2.70 1.51 3.08 9.41
Cytoplasm -0.53 -0.00 -7.85 -15.82 -10.88 2.40 3.22 2.81 1.08 -1.36 5.76 5.82 -5.62 -12.06 -9.47
Cytoplasmic Foci -1.71 10.68 13.81 43.85 7.41 6.75 38.90 38.74 31.68 -2.72 1.82 33.95 44.81 54.25 5.85
Eisosomes 3.64 4.34 4.23 15.03 -9.77 2.21 3.24 3.55 10.80 2.82 3.01 4.42 4.49 17.98 1.00
Endoplasmic Reticulum 1.31 1.91 0.44 -4.39 -6.62 -1.48 -8.31 -11.04 -4.94 4.70 -1.68 -6.62 -8.97 -4.97 2.70
Endosome -2.91 4.34 3.83 16.85 -6.38 -1.09 7.21 8.28 10.92 0.58 -5.51 10.47 10.28 20.48 -1.31
Golgi -3.17 3.31 3.79 9.29 1.78 -4.04 9.33 2.79 5.29 -1.12 -6.70 7.91 5.87 10.70 0.10
Lipid Particles 3.69 4.35 5.51 12.20 7.02 6.11 8.70 10.16 7.65 1.90 6.06 8.54 12.82 16.14 7.62
Mitochondria -2.62 3.77 3.69 10.49 -1.84 -1.84 6.56 6.44 9.18 -2.05 -4.02 7.08 6.87 13.83 -2.84
Mitotic Spindle -1.07 -0.81 -5.32 -4.44 -4.60 -3.78 -1.65 -2.76 -0.13 -1.29 -4.33 -2.78 -6.91 -4.47 -5.07
None 1.68 1.12 1.09 -3.14 -0.14 -1.43 -2.14 -0.89 0.67 1.07 -0.23 -2.06 -2.05 -1.93 -0.13
Nuclear Periphery 1.25 0.06 0.28 -12.53 1.67 1.02 -2.13 -3.22 -15.83 -2.62 1.27 -2.27 -2.19 -18.84 0.38
Nuclear Periphery Foci 1.58 -1.78 0.39 -4.48 4.38 1.45 -7.79 -9.61 -10.23 5.34 0.86 -10.32 -6.20 -7.21 6.76
Nucleolus 1.24 1.87 1.78 5.86 -3.75 -1.80 3.69 4.61 5.48 0.90 -0.97 3.68 3.20 7.60 -2.09
Nucleus 3.78 -13.94 -4.11 -19.01 14.31 0.33 -9.35 -11.53 -11.49 2.70 5.61 -18.67 -12.98 -19.08 12.25
Peroxisomes 1.46 5.67 5.61 13.17 -2.87 -5.02 10.50 8.57 11.72 -0.94 -6.09 11.94 11.46 18.13 -1.87
Vacuole -8.94 -5.30 -19.87 -15.22 -8.60 -5.52 -9.46 -17.68 -16.24 -0.90 -11.58 -9.98 -29.19 -23.54 -4.26
Vacuole Periphery -4.15 3.73 0.68 4.68 -2.53 -2.42 6.70 5.76 4.29 -0.66 -5.23 6.99 0.61 4.64 -2.65
Standard name
Human Ortholog
Description Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pet18

Pet18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available