Standard name
Human Ortholog
Description Nucleoside monophosphate and nucleoside diphosphate kinase; functions in the de novo biosynthesis of pyrimidine deoxyribonucleotides; thymidylate/uridylate kinase that converts nucleoside monophosphates, dTMP and dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside diphosphate kinase that converts nucleoside diphosphates, dTDP and dUDP to dTTP and dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe tmp1; human homolog DTYMK can complement the cdc8 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.99 0.98 0.96 0.9 0.89 0.77 0.78 0.74 0.72 0.98 0.99 0.99 0.94 0.97 0.96 0.87 0.9 0.96 0.95 0.9 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.06 0.28 0.2 0.31 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0.1 0.1 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 2 1 1 1 1 1
Bud 0 0 0 0 0 3 2 6 3 4 13 0 0 0 0 0 0 0 2 3 0 8 4
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cell Periphery 1 2 7 1 2 1 7 4 4 5 9 0 1 2 6 6 4 0 0 0 0 0 0
Cytoplasm 169 237 422 277 335 310 429 430 429 374 422 181 369 347 120 145 175 167 226 405 211 350 391
Endoplasmic Reticulum 3 2 1 0 0 1 1 1 1 0 1 1 1 0 5 5 9 1 0 0 1 7 2
Endosome 0 0 1 0 0 0 3 0 2 4 1 0 0 1 0 0 2 4 2 1 1 1 4
Golgi 3 2 0 1 0 0 1 0 0 0 0 0 0 1 0 0 2 3 3 1 0 2 0
Mitochondria 2 1 0 6 7 66 29 155 110 158 183 1 1 0 1 2 5 1 2 3 0 2 2
Nucleus 1 2 1 1 1 4 11 13 18 5 14 1 1 0 9 2 1 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 2 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 2 0 2 0 0 0 0 0 0 0 0 1 0 4 2
Vac/Vac Membrane 2 4 6 7 27 11 50 56 57 38 31 1 2 5 5 6 5 7 9 0 1 1 0
Unique Cell Count 178 247 428 283 349 343 483 562 550 503 588 184 372 350 128 150 182 193 253 424 222 389 418
Labelled Cell Count 181 251 438 293 372 396 536 671 630 593 679 185 375 356 146 166 203 193 253 424 222 389 418


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.6 6.5 6.1 6.8 4.9 5.7 5.0 5.3 5.0 5.0 6.7 6.9 7.2 8.2 8.3 8.4 5.9 6.4 6.3
Std Deviation (1e-4) 0.9 1.1 1.1 1.6 1.5 1.5 1.8 1.4 1.2 1.4 0.9 1.0 1.2 1.7 1.1 1.2 1.5 1.1 2.1 1.8
Intensity Change (Log2) -0.09 0.07 -0.4 -0.18 -0.38 -0.3 -0.36 -0.38 0.05 0.09 0.16 0.33 0.36 0.37 -0.14 -0.02 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 -0.2 0 0 -0.9 -0.1 0 0 0 0 0 0
Cytoplasm -0.7 -2.3 -5.2 -5.9 -9.9 -9.5 -10.5 -11.3 -0.2 0.8 0.7 -3.1 -1.5 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 9.5 5.2 11.8 9.8 12.7 12.7 0 0 0 0 0 0
Nucleus 0 0 0 2.7 2.7 3.4 0 2.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 4.4 1.7 5.6 5.5 5.7 4.4 3.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2965 1.9084 1.7565 1.5865 1.2326 1.3622 2.8255 3.532 3.5134 2.7865 2.8825 3.1189 4.8785 6.2656 5.8207 5.8546 6.1073 5.8163
Actin 0.0286 0.0023 0.011 0.0148 0.0389 0.0128 0.06 0.001 0.0161 0.0053 0.0212 0.0052 0.0221 0.0004 0.0201 0.0008 0.0014 0.0004
Bud 0.0008 0.0041 0.0005 0.0352 0.0007 0.0002 0.0014 0.0012 0.0015 0.0023 0.0151 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0
Bud Neck 0.0008 0.0001 0.0002 0.0005 0.0008 0.0024 0.0067 0.0002 0.0007 0.0005 0.0005 0.0011 0.0005 0.0001 0.0032 0.0003 0.0004 0.0005
Bud Periphery 0.0008 0.0016 0.0003 0.0154 0.001 0.0002 0.0018 0.0006 0.0014 0.0027 0.0191 0.0005 0.0001 0 0.0003 0 0.0001 0
Bud Site 0.0028 0.0062 0.0009 0.0028 0.0045 0.0001 0.0151 0.0019 0.006 0.002 0.0019 0.0009 0.0003 0.0003 0.001 0.0002 0.0004 0
Cell Periphery 0.0001 0.0001 0.0001 0.0005 0.0001 0 0.0004 0.0001 0.0002 0.0001 0.0003 0 0.0001 0 0.0002 0.0001 0 0
Cytoplasm 0.7676 0.8323 0.9107 0.7429 0.7961 0.8645 0.6571 0.9103 0.8075 0.752 0.639 0.8836 0.8262 0.8593 0.867 0.8753 0.7589 0.8936
Cytoplasmic Foci 0.0171 0.0129 0.0082 0.0124 0.0214 0.0086 0.0255 0.0088 0.0103 0.028 0.0151 0.0131 0.0028 0.004 0.0032 0.0065 0.0266 0.0033
Eisosomes 0.0001 0 0 0.0001 0.0002 0.0001 0.0004 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0087 0.003 0.0053 0.0036 0.0076 0.0028 0.0071 0.0013 0.0035 0.002 0.0044 0.0038 0.0098 0.0029 0.0028 0.0057 0.0046 0.0029
Endosome 0.0264 0.0041 0.0039 0.0117 0.0263 0.012 0.0305 0.0026 0.0123 0.0044 0.0329 0.0079 0.0079 0.0014 0.0034 0.0021 0.012 0.0013
Golgi 0.0033 0.0001 0.0014 0.01 0.0099 0.0033 0.0088 0.0008 0.0052 0.0047 0.0232 0.0042 0.0012 0.0001 0.0024 0.0001 0.0016 0.0001
Lipid Particles 0.0018 0.0004 0.0014 0.0006 0.0051 0.0004 0.0162 0 0.0027 0.0022 0.0292 0.0038 0.0004 0.0001 0.0006 0 0.0062 0
Mitochondria 0.0021 0.0002 0.0004 0.0135 0.0046 0.0059 0.0053 0.0006 0.0053 0.0008 0.0182 0.0064 0.0004 0.0001 0.0014 0.0001 0.0003 0.0001
None 0.0942 0.1225 0.0466 0.1182 0.0443 0.0741 0.1233 0.0627 0.1046 0.1577 0.1566 0.0456 0.1123 0.1265 0.0883 0.0997 0.1299 0.0878
Nuclear Periphery 0.0132 0.0017 0.0021 0.0047 0.015 0.0019 0.0117 0.0009 0.0031 0.0012 0.0044 0.0035 0.0042 0.001 0.001 0.0019 0.0022 0.001
Nucleolus 0.0005 0.0002 0.0001 0.0002 0.0003 0.0001 0.0007 0 0.0004 0.0001 0.0002 0.0001 0 0 0.0001 0 0.0008 0
Nucleus 0.017 0.0034 0.0034 0.0061 0.0044 0.0076 0.0057 0.0032 0.007 0.0033 0.0049 0.0088 0.0096 0.0024 0.0029 0.0043 0.0026 0.0073
Peroxisomes 0.0023 0.0001 0.0011 0.0027 0.0122 0.0002 0.011 0.002 0.0047 0.0256 0.0074 0.0066 0.0001 0.0001 0.0003 0.0002 0.0027 0.0001
Punctate Nuclear 0.0063 0.0012 0.0012 0.0008 0.0027 0.0014 0.0068 0.0006 0.0059 0.0039 0.0037 0.0029 0.0006 0.0006 0.0004 0.0016 0.048 0.0012
Vacuole 0.0042 0.0033 0.0011 0.0025 0.0021 0.0012 0.0029 0.0009 0.0012 0.0009 0.0015 0.0008 0.0007 0.0005 0.0007 0.0008 0.0011 0.0003
Vacuole Periphery 0.0015 0.0002 0.0002 0.001 0.0019 0.0004 0.0015 0.0002 0.0004 0.0002 0.0009 0.0007 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.6785 31.8186 7.8703 22.5367 42.8524 42.35 25.2778 17.0608 20.24 34.8731
Translational Efficiency 1.3119 0.9155 2.7194 1.5699 0.8261 1.3089 1.168 1.4929 1.602 1.2751

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
276 1422 105 1969 1378 2483 2477 1901 1654 3905 2582 3870

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.08 768.07 963.68 845.00 680.74 803.31 922.71 914.06 681.30 790.48 924.38 878.92
Standard Deviation 91.31 110.78 108.83 126.93 80.60 105.36 111.77 117.76 82.49 108.70 111.94 127.28
Intensity Change Log 2 0.167073 0.494389 0.304786 0.238853 0.438773 0.425185 0.203321 0.466917 0.366094

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000111 0.000561 0.000730 0.000416 0.000072 0.000317 0.000095 0.000230 0.000078 0.000406 0.000121 0.000324
Bud Neck 0.031270 0.005457 0.001593 0.006775 0.006583 0.015721 0.006431 0.006226 0.010702 0.011984 0.006235 0.006505
Bud Site 0.016724 0.006994 0.007028 0.025289 0.003350 0.014583 0.010763 0.021274 0.005582 0.011820 0.010611 0.023317
Cell Periphery 0.000274 0.000114 0.000301 0.000131 0.000101 0.000114 0.000036 0.000190 0.000130 0.000114 0.000047 0.000160
Cytoplasm 0.455928* 0.647635* 0.387114* 0.638194* 0.594302* 0.618016* 0.726225* 0.596386* 0.571212* 0.628801* 0.712435* 0.617657*
Cytoplasmic Foci 0.199457* 0.134127 0.021985 0.015935 0.152905 0.097953 0.006110 0.016484 0.160673 0.111126 0.006756 0.016205
Eisosomes 0.000149 0.000042 0.000102 0.000029 0.000060 0.000043 0.000008 0.000054 0.000075 0.000042 0.000012 0.000041
Endoplasmic Reticulum 0.001538 0.002171 0.007003 0.001165 0.000905 0.000963 0.000357 0.001189 0.001011 0.001403 0.000628 0.001177
Endosome 0.013592 0.008669 0.006725 0.003456 0.003784 0.008944 0.001010 0.002246 0.005421 0.008844 0.001242 0.002862
Golgi 0.001461 0.002358 0.000151 0.001829 0.001275 0.001940 0.000292 0.000810 0.001306 0.002092 0.000287 0.001328
Lipid Particles 0.007373 0.003647 0.003712 0.000758 0.004338 0.002622 0.000241 0.000819 0.004845 0.002995 0.000383 0.000788
Mitochondria 0.002079 0.002187 0.000359 0.001141 0.001446 0.004621 0.001391 0.002083 0.001551 0.003734 0.001349 0.001603
Mitotic Spindle 0.000359 0.001707 0.000262 0.025349 0.001490 0.009174 0.021322 0.012748 0.001301 0.006455 0.020466 0.019159
None 0.004541 0.007640 0.002854 0.006880 0.005096 0.004438 0.004776 0.005464 0.005004 0.005604 0.004698 0.006185
Nuclear Periphery 0.000710 0.000429 0.001811 0.000859 0.000288 0.000483 0.000695 0.000695 0.000359 0.000463 0.000741 0.000779
Nuclear Periphery Foci 0.000616 0.001009 0.003754 0.001131 0.000338 0.000223 0.000154 0.000552 0.000384 0.000510 0.000300 0.000847
Nucleolus 0.001309 0.001046 0.000614 0.000697 0.000726 0.001179 0.000284 0.001183 0.000823 0.001130 0.000298 0.000936
Nucleus 0.206384* 0.118699 0.416430* 0.164975* 0.190583* 0.141574* 0.175176* 0.241555* 0.193220* 0.133244* 0.184987* 0.202593*
Peroxisomes 0.004351 0.002901 0.000416 0.000547 0.001873 0.002988 0.000177 0.000790 0.002287 0.002956 0.000186 0.000666
Vacuole 0.050480 0.050911 0.136803 0.101423 0.029915 0.072404 0.043946 0.088191 0.033347 0.064577 0.047722 0.094923
Vacuole Periphery 0.001295 0.001697 0.000255 0.003020 0.000571 0.001701 0.000509 0.000832 0.000692 0.001699 0.000499 0.001945

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.28 -5.67 -2.33 0.71 3.60 -2.94 -1.93 -5.29 1.16 -3.28 -2.37 -3.08 -4.17 0.98 -2.54
Bud Neck 3.80 4.27 3.84 0.49 -5.14 -7.03 0.71 0.55 9.25 -0.28 -0.87 3.37 3.62 9.13 0.13
Bud Site 1.91 1.79 0.31 -4.55 -2.71 -7.48 -3.94 -5.71 0.32 -2.86 -4.59 -1.80 -5.61 -1.83 -4.12
Cell Periphery 3.36 -0.43 3.86 2.18 6.08 -0.46 5.25 -1.32 -0.42 -8.70 0.89 6.34 1.60 0.41 -10.30
Cytoplasm -10.70 5.02 -5.61 9.94 -9.94 -2.82 -6.39 8.42 12.27 16.24 -7.59 -7.74 4.52 15.10 14.42
Cytoplasmic Foci 5.24 15.22 16.09 26.74 1.75 10.32 31.48 29.90 30.67 -8.28 10.09 35.34 33.79 40.42 -10.86
Eisosomes 5.84 1.23 6.49 4.01 7.58 5.88 18.53 5.59 -0.17 -17.51 8.10 15.30 9.66 3.14 -16.71
Endoplasmic Reticulum -1.18 -6.94 0.77 2.78 7.58 -0.21 1.23 -1.64 -1.92 -4.36 -1.55 0.17 -1.37 0.38 -2.85
Endosome 1.33 1.73 3.21 8.21 5.35 -7.44 8.26 5.97 12.41 -3.63 -4.09 6.62 5.39 14.57 -3.94
Golgi -1.68 3.38 1.13 2.87 -1.85 -1.38 4.05 2.69 3.50 -1.07 -2.13 4.75 2.18 4.11 -2.00
Lipid Particles 2.55 2.06 4.59 7.69 6.52 2.21 5.32 4.68 4.81 -8.59 2.70 6.47 6.00 8.01 -6.10
Mitochondria -0.14 5.51 4.09 2.41 -4.91 -5.13 0.99 0.32 4.84 -0.38 -4.75 1.59 1.98 5.80 0.24
Mitotic Spindle -2.29 -0.19 -8.98 -8.21 -8.98 -4.82 -7.48 -5.74 -1.10 2.02 -4.61 -7.68 -9.69 -5.67 0.06
None -0.78 0.94 -0.17 1.42 -4.08 0.84 0.15 -0.86 -2.97 -2.39 -0.45 0.29 -0.75 -0.36 -2.65
Nuclear Periphery 1.87 -7.16 -4.13 -9.83 5.11 -2.59 -19.58 -19.81 -10.38 -2.67 -1.79 -17.69 -19.71 -13.55 -1.61
Nuclear Periphery Foci -1.71 -6.46 -1.82 -0.08 5.77 3.08 2.79 -5.42 -8.10 -7.84 -1.77 0.04 -5.35 -3.65 -5.73
Nucleolus 0.63 2.14 2.18 3.89 0.15 -2.39 3.89 -0.33 1.12 -2.24 -1.85 4.61 1.15 3.01 -2.70
Nucleus 6.63 -7.37 0.67 -13.65 8.41 8.52 -6.10 -11.61 -21.73 -6.66 11.60 -7.10 -7.33 -24.24 0.34
Peroxisomes 1.12 3.36 3.44 5.34 1.05 -1.49 3.86 3.20 6.24 -2.64 -0.97 4.99 4.46 8.26 -3.43
Vacuole 0.11 -6.77 -16.12 -22.42 1.29 -15.03 -15.00 -25.08 -14.89 -13.51 -13.28 -14.94 -34.50 -25.50 -18.05
Vacuole Periphery -1.32 4.80 -0.25 0.58 -2.74 -6.10 1.51 0.79 6.25 -0.64 -6.55 2.41 -1.01 2.95 -2.40