Standard name
Description Nucleoside monophosphate and nucleoside diphosphate kinase; functions in the de novo biosynthesis of pyrimidine deoxyribonucleotides; thymidylate/uridylate kinase that converts nucleoside monophosphates, dTMP and dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside diphosphate kinase that converts nucleoside diphosphates, dTDP and dUDP to dTTP and dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe tmp1; human homolog DTYMK can complement the cdc8 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.99 0.98 0.96 0.9 0.89 – 0.77 0.78 0.74 0.72 0.98 0.99 0.99 0.94 0.97 0.96 0.87 0.9 0.96 0.95 0.9 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.06 – 0.28 0.2 0.31 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0.1 – 0.1 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 1 0 1 0 0 0 0 0 0 2 1 1 1 1 1
Bud 0 0 0 0 0 3 2 – 6 3 4 13 0 0 0 0 0 0 0 2 3 0 8 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 7 1 2 1 7 – 4 4 5 9 0 1 2 6 6 4 0 0 0 0 0 0
Cytoplasm 169 237 422 277 335 310 429 – 430 429 374 422 181 369 347 120 145 175 167 226 405 211 350 391
Endoplasmic Reticulum 3 2 1 0 0 1 1 – 1 1 0 1 1 1 0 5 5 9 1 0 0 1 7 2
Endosome 0 0 1 0 0 0 3 – 0 2 4 1 0 0 1 0 0 2 4 2 1 1 1 4
Golgi 3 2 0 1 0 0 1 – 0 0 0 0 0 0 1 0 0 2 3 3 1 0 2 0
Mitochondria 2 1 0 6 7 66 29 – 155 110 158 183 1 1 0 1 2 5 1 2 3 0 2 2
Nucleus 1 2 1 1 1 4 11 – 13 18 5 14 1 1 0 9 2 1 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 – 2 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 – 1 2 0 2 0 0 0 0 0 0 0 0 1 0 4 2
Vac/Vac Membrane 2 4 6 7 27 11 50 – 56 57 38 31 1 2 5 5 6 5 7 9 0 1 1 0
Unique Cell Count 178 247 428 283 349 343 483 562 550 503 588 184 372 350 128 150 182 193 253 424 222 389 418
Labelled Cell Count 181 251 438 293 372 396 536 671 630 593 679 185 375 356 146 166 203 193 253 424 222 389 418


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.6 6.5 6.1 6.8 4.9 5.7 – 5.0 5.3 5.0 5.0 6.7 6.9 7.2 8.2 8.3 8.4 5.9 6.4 6.3
Std Deviation (1e-4) 0.9 1.1 1.1 1.6 1.5 1.5 1.8 – 1.4 1.2 1.4 0.9 1.0 1.2 1.7 1.1 1.2 1.5 1.1 2.1 1.8
Intensity Change (Log2) – – – -0.09 0.07 -0.4 -0.18 – -0.38 -0.3 -0.36 -0.38 0.05 0.09 0.16 0.33 0.36 0.37 -0.14 -0.02 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 3.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 -0.2 – 0 0 -0.9 -0.1 0 0 0 0 0 0
Cytoplasm -0.7 -2.3 -5.2 -5.9 – -9.9 -9.5 -10.5 -11.3 -0.2 0.8 0.7 -3.1 -1.5 -1.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 9.5 5.2 – 11.8 9.8 12.7 12.7 0 0 0 0 0 0
Nucleus 0 0 0 2.7 – 2.7 3.4 0 2.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 4.4 1.7 5.6 – 5.5 5.7 4.4 3.3 0 0 0 0 0 0