Standard name
Human Ortholog
Description Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.14 0.19 0.21 0.28 0.43 0.52 0.6 – 0.66 0.79 0.78 0.55 0.8 0.93 0.22 0.21 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0.11 0 0.05 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0.08 0.09 0.05 – 0.1 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.24 0.16 0.25 0.27 0.15 0.12 0.07 0.07 – 0.05 0 0 0.08 0 0 0.35 0.34 0.35 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.7 0.68 0.58 0.8 0.71 0.61 0.54 0.41 – 0.37 0.27 0.3 0.43 0.15 0.05 0.52 0.56 0.58 0.89 0.88 0.91 0.86 0.91 0.85
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 0 1 0 0 1 – 0 0 0 2 5 5 2 1 0 1 1 1 0 3 4
Bud 0 4 1 0 0 2 5 6 – 6 3 2 1 2 1 0 0 0 2 6 7 10 9 8
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 2 4 0 0 0 0 2 0 0 4 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 – 0 1 1 0 3 1 0 0 0 0 0 0 0 0 0
Cytoplasm 24 54 68 45 97 191 289 280 – 328 272 334 194 457 563 64 73 48 3 8 4 9 4 5
Endoplasmic Reticulum 3 14 17 0 4 6 12 5 – 4 0 0 9 19 6 10 19 14 3 4 9 8 13 22
Endosome 27 14 18 1 19 5 2 0 – 3 0 0 14 19 3 13 13 16 12 12 9 18 18 27
Golgi 0 1 0 0 0 0 0 0 – 0 0 0 0 1 0 2 2 1 1 2 0 0 3 7
Mitochondria 4 36 11 1 6 36 51 22 – 51 36 28 0 0 0 12 12 8 3 8 1 10 5 12
Nucleus 62 61 92 57 53 53 38 32 – 25 12 12 28 8 2 103 122 103 0 1 0 1 1 2
Nuclear Periphery 0 0 1 0 0 2 9 2 – 2 1 2 0 0 0 0 1 1 0 1 0 3 6 5
Nucleolus 0 1 2 0 0 0 0 1 – 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 5 0 1 0 0 0 – 0 0 1 0 0 3 2 0 6 0 3 0 2 0 2
Vac/Vac Membrane 177 257 210 168 244 271 298 189 – 182 92 127 152 88 33 155 200 171 265 413 395 426 736 628
Unique Cell Count 254 380 363 211 342 442 554 463 497 343 428 350 573 606 297 355 296 298 470 433 498 811 735
Labelled Cell Count 300 444 426 272 426 566 704 540 601 418 507 400 604 621 363 443 369 298 470 433 498 811 735


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 7.2 6.7 6.9 6.7 5.8 5.6 5.8 – 5.0 5.1 5.3 7.0 6.8 6.6 7.5 8.4 9.3 7.9 8.4 8.6
Std Deviation (1e-4) 1.8 1.7 1.4 1.4 1.7 1.8 1.7 1.5 – 1.4 1.5 1.4 1.1 1.1 1.1 1.7 2.1 2.6 1.7 1.6 1.6
Intensity Change (Log2) – – – 0.03 0.0 -0.2 -0.26 -0.22 – -0.41 -0.38 -0.35 0.06 0.02 -0.03 0.16 0.32 0.47 0.23 0.33 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 3.0 7.4 10.2 12.1 – 13.7 16.1 16.6 10.2 18.3 23.4 0.9 0.6 -0.8
Endoplasmic Reticulum -3.2 -2.7 -2.8 -2.1 -3.2 – -3.6 -4.1 -4.5 -1.5 -1.1 -3.7 -0.8 0.4 0
Endosome -2.9 0.4 -3.2 -4.7 -4.8 – -4.1 -4.2 -4.7 -0.6 -1.3 -4.6 -0.4 -0.9 0.3
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.1 3.1 3.6 1.3 – 4.0 4.0 2.3 -3.3 0 0 0.7 0.3 -0.2
Nucleus 0.4 -3.2 -4.9 -7.8 -7.4 – -8.6 -8.2 -9.3 -6.2 -11.6 -12.7 2.6 2.6 2.6
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 5.3 3.7 1.0 -1.2 -4.9 – -6.2 -8.3 -8.0 -3.9 -13.6 -18.2 -1.5 -0.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0021 0 0.0141 0 0.0273 0.0003 0 0.0004 0.0001 0 0 0
Bud – – – – – – 0.0004 0.0043 0.001 0.0004 0.0005 0.0004 0.0003 0.0005 0.0007 0.0003 0.0003 0.0005
Bud Neck – – – – – – 0.0017 0.002 0.0034 0.0013 0.0022 0.0035 0.0009 0.0022 0.0066 0.002 0.0031 0.0021
Bud Periphery – – – – – – 0.0016 0.0021 0.0023 0.0011 0.0015 0.0014 0.0005 0.0009 0.0016 0.0005 0.0008 0.0012
Bud Site – – – – – – 0.0013 0.0115 0.0062 0.0013 0.0016 0.0012 0.0009 0.0023 0.0027 0.0019 0.0006 0.0017
Cell Periphery – – – – – – 0.008 0.0038 0.0038 0.0031 0.0038 0.0039 0.0011 0.0028 0.0023 0.003 0.0021 0.0036
Cytoplasm – – – – – – 0.0272 0.0151 0.0193 0.0131 0.0051 0.0099 0.0531 0.0476 0.0656 0.0089 0.0062 0.0292
Cytoplasmic Foci – – – – – – 0.0083 0.0075 0.022 0.0054 0.0119 0.008 0.0139 0.0077 0.0107 0.0036 0.0071 0.0125
Eisosomes – – – – – – 0.0002 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum – – – – – – 0.0227 0.0141 0.0068 0.0048 0.0057 0.0085 0.0235 0.0112 0.0183 0.0024 0.003 0.0085
Endosome – – – – – – 0.2321 0.1448 0.225 0.1877 0.1735 0.2015 0.256 0.1122 0.2252 0.1562 0.2149 0.1984
Golgi – – – – – – 0.009 0.0025 0.0164 0.0026 0.0122 0.0054 0.0029 0.0019 0.0037 0.0012 0.003 0.0049
Lipid Particles – – – – – – 0.019 0.0053 0.0081 0.0204 0.0213 0.0086 0.0046 0.0037 0.0044 0.003 0.0064 0.0094
Mitochondria – – – – – – 0.0079 0.0032 0.0184 0.005 0.0046 0.0159 0.0011 0.0009 0.0046 0.0004 0.0007 0.0016
None – – – – – – 0.0181 0.008 0.0123 0.0002 0.0018 0.0011 0.0013 0.028 0.0023 0.0001 0.0002 0.0025
Nuclear Periphery – – – – – – 0.0143 0.0082 0.0084 0.0026 0.008 0.0046 0.0059 0.0097 0.0087 0.0023 0.0033 0.0079
Nucleolus – – – – – – 0.0032 0.0025 0.0019 0.0023 0.0036 0.0015 0.0014 0.0015 0.0017 0.0029 0.0016 0.0031
Nucleus – – – – – – 0.0169 0.0075 0.0039 0.0035 0.0054 0.0033 0.0049 0.008 0.0063 0.0036 0.0023 0.0208
Peroxisomes – – – – – – 0.0021 0.0003 0.0083 0.0003 0.0099 0.0007 0.0002 0.0002 0.0002 0.0002 0.0002 0.0003
Punctate Nuclear – – – – – – 0.0009 0.0003 0.0017 0.0001 0.0035 0.0001 0.0002 0.0011 0.0002 0.0001 0.0001 0.0003
Vacuole – – – – – – 0.5352 0.7056 0.5685 0.6953 0.6292 0.6513 0.5745 0.7163 0.5867 0.7569 0.6734 0.6394
Vacuole Periphery – – – – – – 0.0678 0.0512 0.048 0.0494 0.0673 0.0687 0.0529 0.0409 0.0475 0.0507 0.0705 0.0521

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0562 15.3821 13.7921 19.356 12.5492 12.1816 20.2511 24.9306 21.5751 21.6506
Translational Efficiency 0.9988 0.757 0.9406 0.6896 0.844 1.0387 0.7868 0.4487 0.4869 0.4966

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
191 1124 203 1085 1759 1565 1127 1743 1950 2689 1330 2828

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 992.30 830.62 1211.30 989.98 781.68 885.85 1063.50 1032.75 802.31 862.76 1086.06 1016.34
Standard Deviation 142.85 171.14 159.75 159.43 111.03 122.02 136.91 145.33 130.53 147.14 150.35 152.32
Intensity Change Log 2 — -0.256588 0.287708 -0.003377 — 0.180484 0.444170 0.401841 — -0.047545 0.358750 0.189314

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003846 0.000341 0.014765 0.003870 0.000076 0.000023 0.047854 0.003054 0.000446 0.000156 0.042804 0.003367
Bud Neck 0.023513 0.038009 0.016960 0.033636 0.028196 0.009809 0.047605 0.036595 0.027738 0.021597 0.042928 0.035460
Bud Site 0.067154 0.034801 0.132935* 0.071978 0.013806 0.004846 0.103103* 0.082004 0.019031 0.017367 0.107657* 0.078157
Cell Periphery 0.001967 0.008719 0.772309* 0.008038 0.044903 0.004535 0.371757* 0.011178 0.040698 0.006284 0.432894* 0.009973
Cytoplasm 0.116398* 0.096048 0.036636 0.125301* 0.025566 0.004931 0.239176* 0.116739* 0.034463 0.043018 0.208262* 0.120024*
Cytoplasmic Foci 0.042375 0.034628 0.000831 0.051269 0.004582 0.003822 0.004150 0.031472 0.008283 0.016699 0.003643 0.039067
Eisosomes 0.000220 0.000128 0.002995 0.000327 0.000086 0.000021 0.032036 0.000413 0.000099 0.000066 0.027603 0.000380
Endoplasmic Reticulum 0.003373 0.009994 0.011894 0.026990 0.012769 0.010517 0.044600 0.043508 0.011849 0.010298 0.039608 0.037171
Endosome 0.096786 0.081730 0.000689 0.083704 0.027976 0.042072 0.002537 0.098236 0.034716 0.058649 0.002255 0.092661
Golgi 0.014151 0.001721 0.001193 0.006381 0.001048 0.001501 0.002254 0.007133 0.002332 0.001593 0.002092 0.006845
Lipid Particles 0.003989 0.007343 0.000503 0.013976 0.001512 0.001603 0.041060 0.017946 0.001755 0.004002 0.034870 0.016423
Mitochondria 0.004459 0.000868 0.000223 0.000483 0.000776 0.002203 0.000541 0.002029 0.001136 0.001645 0.000493 0.001436
Mitotic Spindle 0.008679 0.000196 0.000467 0.000669 0.000016 0.000158 0.000249 0.000538 0.000865 0.000174 0.000282 0.000588
None 0.000097 0.001969 0.000019 0.000069 0.000091 0.000201 0.000114 0.000045 0.000092 0.000940 0.000100 0.000055
Nuclear Periphery 0.000126 0.000811 0.000115 0.000101 0.000031 0.000737 0.000330 0.000089 0.000040 0.000768 0.000297 0.000094
Nuclear Periphery Foci 0.001175 0.001368 0.000537 0.002870 0.000121 0.000640 0.001847 0.001518 0.000224 0.000945 0.001647 0.002037
Nucleolus 0.000183 0.002013 0.000011 0.000269 0.000393 0.000996 0.000043 0.000320 0.000372 0.001421 0.000038 0.000300
Nucleus 0.003404 0.007926 0.000658 0.003743 0.001210 0.005967 0.006112 0.002627 0.001425 0.006786 0.005280 0.003055
Peroxisomes 0.004769 0.001640 0.000183 0.003552 0.000404 0.000918 0.006737 0.002267 0.000832 0.001220 0.005737 0.002760
Vacuole 0.596385* 0.657519* 0.005848 0.554077* 0.827029* 0.884624* 0.047554 0.527683* 0.804437* 0.789694* 0.041188 0.537809*
Vacuole Periphery 0.006951 0.012229 0.000229 0.008696 0.009407 0.019878 0.000342 0.014607 0.009166 0.016681 0.000324 0.012339

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.23 -3.37 -0.12 -5.27 3.61 5.60 -17.80 -8.34 -8.48 16.53* 1.81 -18.07 -8.02 -9.52 16.67*
Bud Neck -3.88 1.34 -3.01 1.38 -5.51 8.66 -6.18 -3.13 -13.30 3.65 2.76 -5.53 -3.38 -7.18 3.06
Bud Site 2.75 -3.82 -0.57 -8.04 4.22 5.72 -18.04 -18.32 -21.52 3.55 0.75 -18.30 -19.22 -21.13 5.57
Cell Periphery -6.75 -41.41* -5.89 0.66 41.05* 17.69 -36.91* 14.40 -6.65 41.88* 16.57 -43.70 14.67 -4.50 48.26*
Cytoplasm 1.80 5.51 -0.22 -4.19 -10.84 9.09 -31.13 -20.00 -26.87 15.72 -2.39 -26.77 -21.05 -19.33 12.65
Cytoplasmic Foci 1.14 7.30 -1.49 -4.35 -17.06 0.82 0.46 -15.29 -15.67 -15.81 -6.26 4.75 -17.73 -11.87 -22.20
Eisosomes 2.33 -8.75 -2.37 -4.91 8.37 5.37 -21.17 -5.02 -6.13 20.93 2.55 -20.97 -6.46 -7.40 20.75
Endoplasmic Reticulum -5.44 -3.22 -9.11 -6.06 -3.96 2.23 -9.80 -11.95 -13.07 0.32 1.66 -9.88 -13.02 -14.15 0.85
Endosome 0.65 8.05 0.63 -0.07 -22.84 -4.46 12.86 -19.12 -14.12 -30.92* -7.96 14.94 -18.30 -10.20 -38.16*
Golgi 2.65 2.78 1.58 -6.57 -6.81 -0.86 -4.36 -11.10 -8.12 -9.33 1.08 0.26 -7.48 -10.49 -11.29
Lipid Particles -2.79 4.76 -6.93 -3.90 -10.21 -0.32 -11.42 -14.80 -14.48 6.36 -4.52 -11.18 -17.13 -12.78 5.99
Mitochondria 2.18 2.63 2.45 2.52 -2.48 -2.43 0.88 -2.14 0.25 -2.85 -1.31 2.16 -0.76 0.50 -2.89
Mitotic Spindle 2.10 2.04 1.94 -1.05 -0.60 -1.49 -13.07 -1.92 -1.31 -1.06 1.67 1.44 0.44 -1.64 -1.33
None -1.99 1.20 0.77 2.12 -2.89 -0.79 -0.49 1.03 1.18 3.15 -2.18 -0.02 1.05 2.31 2.47
Nuclear Periphery -2.02 0.68 1.33 2.11 0.59 -3.85 -12.95 -6.29 3.53 9.76 -4.13 -12.79 -7.74 3.82 9.77
Nuclear Periphery Foci -0.91 3.79 -4.31 -3.34 -6.12 -1.33 -17.54 -7.61 -2.05 1.56 -2.98 -16.45 -9.80 -3.55 -2.07
Nucleolus -4.40 4.49 -2.30 4.19 -12.18 -2.06 3.01 0.64 2.52 -17.06* -4.10 3.18 0.69 4.79 -20.25
Nucleus -3.15 4.96 0.10 3.62 -8.49 -4.42 -8.65 -4.62 3.01 5.91 -6.81 -7.68 -6.04 4.62 4.23
Peroxisomes 2.15 3.32 0.70 -4.04 -7.99 -1.63 -12.80 -12.97 -3.91 8.74 -1.78 -11.12 -8.62 -5.60 6.50
Vacuole -2.12 24.69 1.87 7.94 -59.25* -8.28 138.67* 34.11* 42.18* -64.53* 2.11 132.28* 34.26 33.03 -83.76*
Vacuole Periphery -2.77 3.24 -1.05 3.50 -13.87 -8.20 14.29 -5.63 4.09 -20.30* -7.95 14.66 -4.11 5.01 -23.69*
Standard name
Human Ortholog
Description Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress
Localization
Cell Percentages vacuole (75%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fen2

Fen2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fen2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available