Standard name
Human Ortholog
Description ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; stimulates utilization by Mip1p DNA polymerase of RNA primers synthesized by Rpo41p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0.14 0 0.13 0.13 0.1 0.11 0.11 0.14
Bud 0 0 0.06 – – 0 – 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.12 – – 0.05 – 0 0.06 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0
Golgi 0 0.09 0 – – 0 – 0 0 0 0 0 0 0 0 – 0.28 0.55 0.23 0.27 0.12 0.25 0.22 0.27
Mitochondria 0.93 0.9 0.94 – – 0.88 – 0.91 0.88 0.91 0.9 0.96 0.9 0.86 0.86 – 0.1 0.14 0.53 0.47 0.5 0.37 0.34 0.28
Nucleus 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 – – 0 – 0 0 0.07 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.07 0.12 – – 0.18 – 0.18 0.24 0.22 0.11 0.12 0 0 0.06 – 0.07 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 – – 0 – 0 0 0 0 0 0.16 0.17 0.22 – 0.38 0.27 0 0.06 0.18 0.15 0.21 0.17
SpindlePole 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.05 0 – – 0 – 0 0 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 – – 1 – 0 0 0 0 2 0 2 1 – 4 0 31 19 6 10 7 17
Bud 1 0 1 – – 2 – 3 3 4 0 5 1 2 6 – 1 0 1 0 1 0 0 2
Bud Neck 1 0 0 – – 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0
Bud Site 0 0 0 – – 1 – 0 2 0 0 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 – – 1 – 0 1 1 1 0 0 1 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 3 2 – – 5 – 2 6 1 4 1 1 2 1 – 1 2 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 0 – – 0 – 1 0 0 1 0 0 0 0 – 0 1 0 0 0 0 0 1
Endosome 1 0 0 – – 0 – 0 0 0 1 0 0 4 1 – 0 2 7 2 0 4 1 3
Golgi 2 13 0 – – 0 – 0 0 0 0 1 4 2 5 – 8 12 59 40 7 22 16 34
Mitochondria 229 136 16 – – 92 – 82 85 139 81 140 93 108 115 – 3 3 134 71 31 33 24 35
Nucleus 1 1 0 – – 0 – 0 0 1 0 1 0 1 0 – 0 0 0 0 0 1 0 0
Nuclear Periphery 5 3 1 – – 2 – 2 4 11 1 4 1 0 1 – 0 0 1 0 0 0 0 0
Nucleolus 25 10 2 – – 19 – 16 23 33 10 18 3 5 8 – 2 1 1 3 1 0 1 1
Peroxisomes 2 4 0 – – 4 – 0 2 6 0 1 16 21 29 – 11 6 9 9 11 13 15 21
SpindlePole 0 0 0 – – 0 – 1 0 0 1 0 2 0 0 – 0 0 0 0 0 0 0 2
Vac/Vac Membrane 16 7 0 – – 1 – 0 2 1 0 2 2 4 2 – 2 0 5 4 0 1 0 5
Unique Cell Count 246 151 17 104 90 97 152 90 146 103 125 133 29 22 254 154 62 90 72 128
Labelled Cell Count 284 181 22 128 107 128 197 100 176 123 152 169 32 27 254 154 62 90 72 128


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.0 27.5 12.9 – – 17.3 – 16.9 15.9 17.6 17.3 18.5 18.5 19.2 19.1 – 23.1 22.8 20.3 21.6 20.3
Std Deviation (1e-4) 5.5 7.3 4.2 – – 4.9 – 4.8 5.6 5.4 5.6 5.9 5.1 6.4 5.1 – 7.8 8.0 6.1 9.4 8.0
Intensity Change (Log2) – – – – – 0.43 – 0.39 0.31 0.45 0.43 0.52 0.52 0.58 0.57 – 0.84 0.82 0.66 0.75 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud Neck – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Bud Site – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Endoplasmic Reticulum – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Endosome – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Golgi – – 0 – 0 0 0 0 0 0 0 0 – 0 3.7
Mitochondria – – -0.7 – -0.4 -0.8 -0.4 -0.5 0.3 -0.5 -0.9 -0.9 – -5.6 -5.0
Nucleus – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Nucleolus – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes – – 0 – 0 0 0 0 0 0 0 0 – 0 0
SpindlePole – – 0 – 0 0 0 0 0 0 0 0 – 0 0
Vacuole – – 0 – 0 0 0 0 0 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.0812 19.2826 17.3652 12.7594 16.3868 19.4506 16.8185 18.0856 16.9078 15.3692 15.8144 16.7393 18.6235 21.4306 20.3914 20.7134 15.4217 19.8183
Actin 0.0399 0.0244 0.0084 0.0486 0.1706 0.0404 0.0063 0.0317 0.0352 0.0307 0.0379 0.0168 0.0093 0.0194 0.0218 0.0018 0.0152 0.0059
Bud 0.0006 0 0.0002 0.0008 0.0003 0 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0
Bud Neck 0.0003 0.0001 0.0001 0.0037 0.0004 0 0.0005 0.0001 0.0024 0.0002 0.0001 0.0004 0.0003 0.0001 0.0001 0 0.0001 0
Bud Periphery 0.0008 0 0.0002 0.0013 0.0004 0 0.0001 0.0003 0.0004 0.0001 0.0002 0.0002 0.0004 0.0002 0.0001 0 0.0002 0
Bud Site 0.0018 0.0001 0.0008 0.0027 0.0013 0 0.0004 0.0003 0.0008 0.0013 0.0002 0.0002 0.0014 0.0003 0.0001 0 0.0003 0
Cell Periphery 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0006 0.0001
Cytoplasm 0.0298 0 0.0016 0.0001 0 0 0.001 0 0.0001 0 0.0001 0.0001 0.0018 0 0 0 0.0003 0
Cytoplasmic Foci 0.0285 0.0041 0.0066 0.0786 0.0017 0.0006 0.0179 0.0069 0.007 0.009 0.0053 0.0082 0.0148 0.0068 0.0053 0.0007 0.0082 0.0018
Eisosomes 0.0015 0.0005 0.0004 0.0006 0.0019 0.0004 0.0004 0.0028 0.0016 0.0005 0.0006 0.0008 0.0004 0.0004 0.0007 0.0004 0.0007 0.0004
Endoplasmic Reticulum 0.0062 0 0.0001 0 0 0 0.001 0 0.0002 0 0.0002 0.0002 0.0006 0 0 0 0.0001 0
Endosome 0.022 0.0051 0.0083 0.0032 0.0008 0.0009 0.0128 0.0066 0.0078 0.0008 0.0131 0.0069 0.014 0.0072 0.0041 0.0014 0.0074 0.002
Golgi 0.0098 0.0383 0.0322 0.0097 0.0126 0.0155 0.0097 0.0489 0.0393 0.0044 0.0301 0.0443 0.05 0.0641 0.0477 0.0081 0.0596 0.0468
Lipid Particles 0.1248 0.0978 0.0656 0.0628 0.0034 0.0153 0.1174 0.1021 0.0931 0.0629 0.0297 0.0459 0.0775 0.1238 0.0489 0.0583 0.0934 0.0519
Mitochondria 0.6121 0.773 0.7936 0.5914 0.7539 0.8883 0.7523 0.7194 0.7267 0.8189 0.794 0.811 0.7179 0.7056 0.8016 0.8948 0.7245 0.869
None 0.019 0 0.0001 0.0001 0.0001 0 0.0007 0 0.0001 0 0.0001 0.0001 0.0029 0 0 0 0.0009 0
Nuclear Periphery 0.0073 0 0.002 0 0 0 0.0057 0.0001 0.0024 0 0.0002 0.0007 0.0129 0.0001 0.0001 0.0001 0.0004 0
Nucleolus 0.0034 0.0009 0.0002 0.0004 0 0.0001 0.0154 0.0003 0.0018 0.0002 0.0001 0.0016 0.0073 0.0005 0.0007 0.0002 0.028 0.0004
Nucleus 0.0025 0.0001 0.0203 0.0001 0 0 0.0013 0.0001 0.0001 0 0.0001 0.0002 0.0038 0.0001 0.0001 0 0.003 0
Peroxisomes 0.0626 0.0374 0.0332 0.1918 0.0523 0.0339 0.0483 0.0618 0.0673 0.0694 0.0825 0.0464 0.0483 0.0574 0.0588 0.0251 0.0259 0.0153
Punctate Nuclear 0.0085 0 0.0001 0.0006 0 0 0.0007 0 0.0001 0 0.0002 0.0002 0.0131 0 0 0 0.0005 0
Vacuole 0.0034 0.0025 0.0038 0.0004 0 0.0003 0.0015 0.0021 0.0016 0.0002 0.0006 0.0036 0.0079 0.0015 0.0007 0.0006 0.0049 0.0003
Vacuole Periphery 0.0149 0.0154 0.0218 0.0029 0.0002 0.0039 0.0063 0.016 0.0114 0.0012 0.0046 0.0122 0.0145 0.012 0.009 0.0083 0.0257 0.0061

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 120.3468 96.7035 127.3708 130.5956 133.2433 116.2258 135.0543 138.5849 137.3602 120.3444
Translational Efficiency 3.6351 4.2487 2.7681 3.1094 2.9348 4.301 3.141 3.1234 2.7194 3.8015

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1791 106 1069 1343 1612 1459 1183 1645 3403 1565 2252 2988

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1159.24 1554.10 1964.73 2015.97 1520.49 1413.04 1976.37 1909.03 1330.36 1422.59 1970.84 1957.10
Standard Deviation 281.98 429.90 440.20 542.88 362.00 334.98 504.12 465.38 369.40 344.07 474.89 504.51
Intensity Change Log 2 — 0.422900 0.761152 0.798295 — -0.105734 0.378317 0.328303 — 0.146985 0.556511 0.550605

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.027147 0.036890 0.018719 0.071761 0.010502 0.034129 0.046338 0.103183* 0.019262 0.034316 0.033227 0.089060
Bud Neck 0.002031 0.005952 0.004168 0.003036 0.000998 0.001255 0.002617 0.001341 0.001542 0.001573 0.003353 0.002103
Bud Site 0.002030 0.006006 0.002175 0.002211 0.000396 0.000544 0.002012 0.001079 0.001256 0.000914 0.002090 0.001588
Cell Periphery 0.000128 0.000220 0.000063 0.000184 0.000041 0.000060 0.000068 0.000092 0.000087 0.000071 0.000066 0.000133
Cytoplasm 0.001229 0.002861 0.000659 0.000870 0.000471 0.000916 0.000873 0.000850 0.000870 0.001048 0.000771 0.000859
Cytoplasmic Foci 0.048402 0.074696 0.077492 0.041883 0.048354 0.058359 0.063663 0.025548 0.048379 0.059466 0.070228 0.032890
Eisosomes 0.007461 0.007661 0.006397 0.006269 0.009960 0.008406 0.017475 0.009951 0.008645 0.008355 0.012216 0.008296
Endoplasmic Reticulum 0.000246 0.000569 0.000148 0.000696 0.000114 0.000200 0.000178 0.000626 0.000183 0.000225 0.000164 0.000658
Endosome 0.005760 0.010932 0.003077 0.006083 0.001010 0.003373 0.001796 0.003142 0.003510 0.003885 0.002404 0.004464
Golgi 0.095536 0.092265 0.100739 0.170758* 0.062736 0.118251* 0.096450 0.129950* 0.079999 0.116491* 0.098486 0.148292*
Lipid Particles 0.054934 0.047845 0.052501 0.053784 0.028696 0.035010 0.044075 0.011368 0.042505 0.035879 0.048074 0.030432
Mitochondria 0.668219* 0.548938* 0.680478* 0.597095* 0.779519* 0.640548* 0.647487* 0.657204* 0.720942* 0.634343* 0.663147* 0.630187*
Mitotic Spindle 0.000564 0.012328 0.005271 0.003063 0.000733 0.001803 0.002229 0.001045 0.000644 0.002516 0.003673 0.001952
None 0.002432 0.002758 0.004198 0.002631 0.003912 0.001970 0.003127 0.001560 0.003133 0.002024 0.003635 0.002042
Nuclear Periphery 0.000250 0.000703 0.001749 0.000917 0.000291 0.000222 0.000662 0.000191 0.000270 0.000255 0.001178 0.000517
Nuclear Periphery Foci 0.000325 0.000578 0.000558 0.000435 0.000201 0.000192 0.000178 0.000111 0.000266 0.000218 0.000358 0.000257
Nucleolus 0.000743 0.002199 0.001430 0.000348 0.000706 0.000501 0.000702 0.000202 0.000725 0.000616 0.001048 0.000268
Nucleus 0.000106 0.000768 0.000081 0.000110 0.000453 0.000056 0.000081 0.000034 0.000271 0.000105 0.000081 0.000068
Peroxisomes 0.079175 0.139805* 0.028209 0.026935 0.047756 0.091057 0.066143 0.049069 0.064292 0.094359 0.048136 0.039121
Vacuole 0.001564 0.003909 0.005441 0.003577 0.001507 0.001782 0.001554 0.001477 0.001537 0.001926 0.003399 0.002421
Vacuole Periphery 0.001717 0.002117 0.006445 0.007351 0.001643 0.001366 0.002294 0.001975 0.001682 0.001417 0.004264 0.004392

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.88 2.81 -9.35 -3.01 -11.22 -8.28 -10.72 -20.13 -13.17 -10.29 -5.21 -6.00 -20.67 -13.41 -15.06
Bud Neck -1.82 -3.61 -2.20 1.42 1.82 -1.30 -4.53 -1.95 -0.48 3.65 -0.30 -5.24 -2.33 -1.76 3.64
Bud Site -1.41 -0.21 -0.45 1.35 -0.12 -1.14 -2.52 -2.85 -2.32 1.41 0.78 -1.68 -1.18 -2.02 1.02
Cell Periphery -0.82 2.46 -1.76 0.33 -5.22 -2.33 -2.28 -5.11 -4.46 -1.89 0.94 1.33 -2.73 -4.53 -5.48
Cytoplasm -1.04 2.58 1.49 1.23 -2.92 -11.17 -6.93 -1.60 -0.85 -0.92 -1.10 0.85 -0.89 -0.34 -1.25
Cytoplasmic Foci -2.66 -7.79 2.31 3.28 9.44 -3.17 -4.45 9.75 12.72 13.03 -4.11 -8.65 8.72 10.46 15.86
Eisosomes -0.09 1.17 1.41 0.79 0.16 1.78 -5.44 0.02 -1.75 5.44 0.38 -4.16 0.59 0.09 4.70
Endoplasmic Reticulum -1.88 3.85 -5.09 -0.64 -6.35 -4.33 -3.57 -1.68 -1.40 -1.47 -1.80 1.22 -2.76 -2.51 -2.88
Endosome -1.57 2.91 -0.37 1.49 -4.04 -6.60 -1.81 -5.64 0.45 -2.40 -0.68 2.10 -1.88 -1.16 -4.51
Golgi 0.25 -0.83 -10.41 -5.04 -9.07 -9.83 -6.12 -11.93 -1.76 -5.15 -7.05 -4.41 -14.98 -5.43 -9.97
Lipid Particles 0.68 0.52 0.24 -0.56 -0.26 -2.10 -4.24 8.44 9.07 9.93 2.22 -1.86 4.74 1.85 5.99
Mitochondria 3.29 -0.93 5.60 -1.35 6.03 12.25 10.98 11.61 -1.36 -0.75 8.42 6.54 10.95 0.42 3.57
Mitotic Spindle -1.55 -4.79 -3.57 1.18 1.52 -2.26 -2.80 -0.95 1.60 2.21 -2.86 -5.58 -3.68 0.56 2.43
None -0.73 -8.28 -1.50 0.30 7.19 12.30 4.39 15.60 4.51 12.65 10.01 -3.66 11.07 -0.18 13.02
Nuclear Periphery -1.80 -6.45 -6.34 -0.80 3.24 3.09 -7.11 4.20 1.71 9.30 0.62 -7.95 -5.04 -5.05 5.32
Nuclear Periphery Foci -1.42 -2.53 -0.67 0.68 0.63 0.21 0.58 1.58 1.42 1.19 1.08 -1.88 0.06 -0.46 1.09
Nucleolus -0.89 -1.69 2.51 1.10 2.68 1.12 0.04 2.91 2.73 4.58 0.55 -1.50 3.94 2.25 3.88
Nucleus -1.11 1.01 -0.59 1.07 -1.09 0.97 0.91 1.01 2.43 1.57 0.84 0.96 0.98 0.60 0.20
Peroxisomes -2.72 11.24 11.39 5.15 0.41 -8.12 -3.61 -0.31 7.94 3.39 -5.91 4.59 7.97 11.51 2.92
Vacuole -1.36 -5.55 -3.47 0.47 2.13 -0.64 -0.17 0.02 0.53 0.13 -1.09 -5.11 -2.56 -0.91 2.05
Vacuole Periphery -0.88 -7.41 -8.36 -6.31 -0.99 1.91 -2.62 -1.28 -2.42 0.96 2.16 -7.77 -7.99 -8.72 -0.28
Standard name
Human Ortholog
Description ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; stimulates utilization by Mip1p DNA polymerase of RNA primers synthesized by Rpo41p
Localization
Cell Percentages mitochondrion (87%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Rim1

Rim1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available