ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 28 | 19 | 6 | 5 | 17 | 5 | 13 | 21 | 1 | 10 | 11 | 20 | 16 | 9 | 5 | 27 | 16 | 9 | 11 | 17 | 6 | 4 | 13 | 28 |
Bud | 5 | 2 | 6 | 4 | 11 | 17 | 38 | 45 | 12 | 44 | 39 | 47 | 6 | 5 | 5 | 2 | 7 | 0 | 1 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 9 | 1 | 6 | 2 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 9 | 5 | 7 | 13 | 7 | 11 | 12 | 4 | 2 | 8 | 8 | 9 | 13 | 18 | 10 | 13 | 7 | 0 | 3 | 0 | 1 | 1 | 2 |
Endoplasmic Reticulum | 6 | 4 | 1 | 2 | 1 | 4 | 0 | 2 | 0 | 0 | 2 | 1 | 5 | 3 | 4 | 2 | 4 | 5 | 0 | 0 | 0 | 1 | 2 | 1 |
Endosome | 11 | 2 | 0 | 8 | 34 | 27 | 42 | 51 | 5 | 8 | 12 | 7 | 5 | 9 | 12 | 3 | 2 | 3 | 24 | 19 | 18 | 5 | 11 | 11 |
Golgi | 148 | 113 | 17 | 35 | 128 | 41 | 38 | 42 | 4 | 7 | 7 | 4 | 106 | 95 | 54 | 123 | 78 | 80 | 316 | 156 | 108 | 71 | 201 | 199 |
Mitochondria | 324 | 147 | 144 | 200 | 209 | 359 | 469 | 583 | 247 | 425 | 361 | 443 | 239 | 288 | 245 | 9 | 12 | 10 | 12 | 8 | 3 | 2 | 4 | 5 |
Nucleus | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 3 | 2 | 1 | 0 | 2 | 5 | 2 | 4 | 3 | 2 | 1 | 5 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 25 | 7 | 2 | 12 | 9 | 32 | 50 | 78 | 27 | 33 | 27 | 21 | 6 | 13 | 6 | 8 | 7 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 0 | 0 | 2 | 11 | 40 | 95 | 119 | 26 | 91 | 42 | 105 | 4 | 13 | 24 | 0 | 0 | 0 | 15 | 4 | 13 | 4 | 6 | 6 |
SpindlePole | 3 | 0 | 1 | 2 | 3 | 9 | 27 | 34 | 8 | 42 | 18 | 26 | 0 | 0 | 0 | 4 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 32 | 17 | 8 | 1 | 4 | 7 | 10 | 10 | 3 | 3 | 2 | 1 | 16 | 8 | 4 | 15 | 12 | 15 | 5 | 4 | 0 | 1 | 3 | 2 |
Unique Cell Count | 374 | 203 | 152 | 220 | 304 | 413 | 598 | 773 | 283 | 504 | 420 | 529 | 269 | 324 | 299 | 146 | 107 | 104 | 390 | 223 | 154 | 94 | 249 | 262 |
Labelled Cell Count | 592 | 327 | 193 | 280 | 440 | 552 | 801 | 1015 | 342 | 676 | 536 | 696 | 418 | 462 | 379 | 206 | 153 | 145 | 390 | 223 | 154 | 94 | 249 | 262 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.1 | 16.9 | 9.1 | 11.9 | 12.4 | 10.8 | 9.9 | 10.0 | 8.8 | 9.1 | 8.9 | 8.8 | 14.6 | 14.3 | 13.5 | 22.8 | 21.3 | 20.1 | 14.2 | 16.1 | 16.3 |
Std Deviation (1e-4) | 2.8 | 3.8 | 1.8 | 2.0 | 2.2 | 1.7 | 1.7 | 1.6 | 1.7 | 1.6 | 2.0 | 3.4 | 2.5 | 2.5 | 2.7 | 5.5 | 5.9 | 6.2 | 2.6 | 2.9 | 3.1 |
Intensity Change (Log2) | – | – | – | 0.39 | 0.45 | 0.26 | 0.13 | 0.14 | -0.05 | 0.0 | -0.03 | -0.04 | 0.69 | 0.66 | 0.57 | 1.33 | 1.23 | 1.15 | 0.65 | 0.83 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | 0.9 | 0 | 0 | 4.0 | 3.1 | 1.6 |
Bud | 0 | -0.2 | 0.1 | 1.1 | 0.9 | 0.1 | 1.9 | 2.1 | 2.0 | 0 | 0 | 0 | 0 | 0.9 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 1.2 | 1.4 | 2.8 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4.3 | 3.2 | 3.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.3 | 6.7 | -0.4 | -2.0 | -2.7 | -4.5 | -5.6 | -5.0 | 0 | 6.1 | 4.3 | 1.9 | 12.6 | 10.1 | 10.6 |
Mitochondria | -1.4 | -6.3 | -2.6 | -4.6 | -5.3 | -2.5 | -3.3 | -2.9 | -3.5 | -2.0 | -2.1 | -3.7 | -15.3 | -13.5 | -13.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.1 | 0 | 2.9 | 3.1 | 3.5 | 3.3 | 2.5 | 2.5 | 1.6 | 0 | 0 | 0 | 0 | 0 | 3.7 |
Peroxisomes | 0 | 0 | 4.0 | 5.3 | 5.2 | 3.9 | 5.6 | 4.1 | 6.0 | 0 | 0 | 3.6 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2.2 | 2.2 | 0 | 3.4 | 2.1 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | -1.6 | 0 | 1.6 | 1.8 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.2196 | 9.8212 | 9.1926 | 9.6474 | 8.7297 | 9.1 | 8.5097 | 10.9901 | 10.391 | 10.7265 | 9.7846 | 9.8393 | 10.762 | 13.464 | 12.9707 | 13.0244 | 11.8627 | 12.809 |
Actin | 0.0541 | 0.0174 | 0.0158 | 0.0154 | 0.0033 | 0.0471 | 0.0566 | 0.0129 | 0.0238 | 0.0169 | 0.0231 | 0.0164 | 0.0558 | 0.0171 | 0.0254 | 0.0188 | 0.0039 | 0.0033 |
Bud | 0.0003 | 0 | 0.0003 | 0.0014 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Neck | 0.0005 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0041 | 0.0001 | 0.0003 | 0.0001 | 0.0018 | 0.0001 |
Bud Periphery | 0.0003 | 0 | 0.0004 | 0.0034 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0014 | 0.0001 | 0.0017 | 0.0039 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0045 | 0.0001 | 0.0003 | 0.0001 | 0.0009 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0007 | 0.0004 | 0.0011 | 0.0004 | 0.0002 | 0.0002 | 0.0048 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0029 | 0.0004 | 0.0007 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasmic Foci | 0.0495 | 0.0507 | 0.04 | 0.0427 | 0.0544 | 0.0384 | 0.0548 | 0.0513 | 0.043 | 0.048 | 0.0435 | 0.0341 | 0.0614 | 0.0594 | 0.0464 | 0.0448 | 0.0374 | 0.0277 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0007 | 0.0001 | 0.0007 | 0.0011 | 0.0004 | 0.0001 | 0.0016 | 0.0001 | 0.0007 | 0.0013 | 0.0001 | 0.0003 | 0.0022 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0001 |
Endosome | 0.0889 | 0.0743 | 0.0849 | 0.083 | 0.0717 | 0.0632 | 0.0659 | 0.0698 | 0.0598 | 0.0774 | 0.0619 | 0.0532 | 0.0914 | 0.0851 | 0.0886 | 0.174 | 0.0798 | 0.0663 |
Golgi | 0.6435 | 0.7965 | 0.7573 | 0.7863 | 0.7066 | 0.704 | 0.6631 | 0.824 | 0.8185 | 0.7997 | 0.7733 | 0.8476 | 0.642 | 0.774 | 0.7834 | 0.6672 | 0.7786 | 0.866 |
Lipid Particles | 0.051 | 0.0128 | 0.0169 | 0.0147 | 0.1163 | 0.0534 | 0.0454 | 0.0141 | 0.0138 | 0.0365 | 0.0529 | 0.0176 | 0.0489 | 0.0438 | 0.021 | 0.0477 | 0.0593 | 0.0159 |
Mitochondria | 0.0442 | 0.0264 | 0.0443 | 0.0076 | 0.0081 | 0.049 | 0.0411 | 0.0111 | 0.0119 | 0.0081 | 0.0219 | 0.0095 | 0.0121 | 0.0047 | 0.0134 | 0.0034 | 0.0054 | 0.0062 |
None | 0.0003 | 0 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.002 | 0 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0033 | 0 | 0.0013 | 0.0006 | 0.0008 | 0 |
Nuclear Periphery | 0.0019 | 0.0001 | 0.0009 | 0.0014 | 0.007 | 0.0001 | 0.0022 | 0 | 0.0003 | 0.0014 | 0.0001 | 0.0011 | 0.0054 | 0.0001 | 0.0013 | 0.0003 | 0.0001 | 0.0001 |
Nucleolus | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0004 | 0 | 0.0007 | 0 | 0.0007 | 0 |
Nucleus | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0.0012 | 0 | 0.0006 | 0 | 0.0002 | 0 |
Peroxisomes | 0.046 | 0.0177 | 0.0252 | 0.032 | 0.0269 | 0.041 | 0.055 | 0.0156 | 0.0262 | 0.0057 | 0.021 | 0.0136 | 0.051 | 0.0095 | 0.01 | 0.0333 | 0.0237 | 0.0104 |
Punctate Nuclear | 0.004 | 0 | 0.0015 | 0.0003 | 0.0014 | 0.0004 | 0.002 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0019 | 0.0074 | 0.0001 | 0.0024 | 0.0007 | 0.0029 | 0 |
Vacuole | 0.0053 | 0.0005 | 0.0021 | 0.0023 | 0.0003 | 0.0004 | 0.001 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0008 | 0.0011 | 0.0018 | 0.0008 | 0.0026 | 0.001 | 0.0008 |
Vacuole Periphery | 0.0061 | 0.0028 | 0.0055 | 0.0017 | 0.0027 | 0.0019 | 0.0015 | 0.0006 | 0.0008 | 0.0011 | 0.0012 | 0.0021 | 0.004 | 0.0034 | 0.0019 | 0.0054 | 0.0026 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.3424 | 48.8051 | 47.6519 | 57.9556 | 57.7461 | 74.6683 | 85.0582 | 60.4762 | 49.4109 | 64.4676 |
Translational Efficiency | 1.5409 | 2.2336 | 1.807 | 1.3422 | 1.4168 | 1.1351 | 1.088 | 1.1113 | 1.0007 | 1.1797 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1573 | 1456 | 224 | 493 | 453 | 1493 | 237 | 1321 | 2026 | 2949 | 461 | 1814 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1141.25 | 1315.26 | 1537.59 | 1532.57 | 1231.97 | 1268.37 | 1639.02 | 1443.02 | 1161.53 | 1291.52 | 1589.74 | 1467.36 |
Standard Deviation | 199.95 | 223.70 | 190.31 | 256.17 | 173.54 | 202.30 | 184.85 | 223.06 | 198.00 | 214.42 | 194.25 | 235.92 |
Intensity Change Log 2 | — | 0.204733 | 0.430056 | 0.425338 | — | 0.042009 | 0.411866 | 0.228124 | — | 0.122554 | 0.420642 | 0.326330 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.029763 | 0.039332 | 0.058236 | 0.036875 | 0.031090 | 0.022274 | 0.032483 | 0.041370 | 0.030060 | 0.030696 | 0.044997 | 0.040149 |
Bud Neck | 0.000867 | 0.000464 | 0.002752 | 0.001816 | 0.002084 | 0.000354 | 0.001882 | 0.000880 | 0.001139 | 0.000409 | 0.002305 | 0.001134 |
Bud Site | 0.000545 | 0.000535 | 0.001349 | 0.002454 | 0.001547 | 0.000456 | 0.001035 | 0.002188 | 0.000769 | 0.000495 | 0.001187 | 0.002260 |
Cell Periphery | 0.000072 | 0.000068 | 0.000186 | 0.000288 | 0.000758 | 0.000062 | 0.000154 | 0.000183 | 0.000225 | 0.000065 | 0.000170 | 0.000212 |
Cytoplasm | 0.000658 | 0.000608 | 0.000313 | 0.000472 | 0.000564 | 0.000534 | 0.000256 | 0.000849 | 0.000637 | 0.000570 | 0.000284 | 0.000747 |
Cytoplasmic Foci | 0.135833 | 0.141969 | 0.098013 | 0.081764 | 0.178316 | 0.183763* | 0.074285 | 0.090810 | 0.145332 | 0.163128 | 0.085814 | 0.088351 |
Eisosomes | 0.000533 | 0.000436 | 0.000389 | 0.000299 | 0.000323 | 0.000251 | 0.000268 | 0.000361 | 0.000486 | 0.000343 | 0.000327 | 0.000344 |
Endoplasmic Reticulum | 0.000375 | 0.000417 | 0.001433 | 0.001674 | 0.000655 | 0.000299 | 0.001523 | 0.001067 | 0.000438 | 0.000357 | 0.001479 | 0.001232 |
Endosome | 0.048257 | 0.060360 | 0.067330 | 0.094865 | 0.079500 | 0.075844 | 0.072870 | 0.103049 | 0.055242 | 0.068199 | 0.070178 | 0.100825 |
Golgi | 0.625623* | 0.630184* | 0.618173* | 0.637748* | 0.593475* | 0.610043* | 0.704719* | 0.642384* | 0.618435* | 0.619987* | 0.662666* | 0.641124* |
Lipid Particles | 0.104987* | 0.091513 | 0.114722* | 0.064420 | 0.064058 | 0.053441 | 0.068221 | 0.063130 | 0.095835 | 0.072238 | 0.090816 | 0.063480 |
Mitochondria | 0.033937 | 0.019628 | 0.012764 | 0.021010 | 0.026693 | 0.036577 | 0.009604 | 0.021875 | 0.032317 | 0.028209 | 0.011139 | 0.021640 |
Mitotic Spindle | 0.000331 | 0.000990 | 0.003769 | 0.004162 | 0.001714 | 0.000698 | 0.001684 | 0.001320 | 0.000640 | 0.000842 | 0.002697 | 0.002093 |
None | 0.000133 | 0.000105 | 0.000139 | 0.000140 | 0.000082 | 0.000078 | 0.000133 | 0.000109 | 0.000122 | 0.000091 | 0.000136 | 0.000118 |
Nuclear Periphery | 0.000085 | 0.000205 | 0.000904 | 0.000644 | 0.000136 | 0.000144 | 0.001607 | 0.000384 | 0.000096 | 0.000175 | 0.001266 | 0.000454 |
Nuclear Periphery Foci | 0.000227 | 0.000185 | 0.000950 | 0.000960 | 0.000240 | 0.000170 | 0.001158 | 0.001032 | 0.000230 | 0.000177 | 0.001057 | 0.001012 |
Nucleolus | 0.000048 | 0.000018 | 0.000020 | 0.000034 | 0.000019 | 0.000012 | 0.000023 | 0.000050 | 0.000041 | 0.000015 | 0.000021 | 0.000045 |
Nucleus | 0.000031 | 0.000018 | 0.000035 | 0.000027 | 0.000021 | 0.000015 | 0.000024 | 0.000022 | 0.000029 | 0.000016 | 0.000030 | 0.000024 |
Peroxisomes | 0.014193 | 0.007762 | 0.001976 | 0.003794 | 0.005804 | 0.008302 | 0.002362 | 0.002612 | 0.012318 | 0.008035 | 0.002175 | 0.002933 |
Vacuole | 0.001618 | 0.001974 | 0.008804 | 0.027090 | 0.006958 | 0.002878 | 0.013847 | 0.015260 | 0.002812 | 0.002432 | 0.011396 | 0.018475 |
Vacuole Periphery | 0.001887 | 0.003229 | 0.007744 | 0.019464 | 0.005965 | 0.003806 | 0.011862 | 0.011065 | 0.002798 | 0.003521 | 0.009861 | 0.013348 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.51 | -2.89 | -1.24 | 0.38 | 1.94 | 1.76 | -0.18 | -1.75 | -4.57 | -1.27 | -0.23 | -2.49 | -2.74 | -2.68 | 0.75 |
Bud Neck | 1.61 | -2.46 | -2.15 | -3.77 | 1.15 | 2.08 | 0.22 | 1.42 | -3.49 | 2.58 | 2.72 | -2.42 | 0.02 | -4.94 | 2.78 |
Bud Site | 0.11 | -2.37 | -4.64 | -4.79 | -2.35 | 2.36 | 1.04 | -0.99 | -3.23 | -2.09 | 1.63 | -1.72 | -3.46 | -4.31 | -2.52 |
Cell Periphery | 0.43 | -2.18 | -2.53 | -2.59 | -1.04 | 1.10 | 0.96 | 0.91 | -4.71 | -0.86 | 1.14 | 0.39 | 0.09 | -4.93 | -1.06 |
Cytoplasm | 0.69 | 5.73 | 2.55 | 1.94 | -3.03 | 0.33 | 3.29 | -1.19 | -1.39 | -2.54 | 1.25 | 7.01 | -0.68 | -1.08 | -2.71 |
Cytoplasmic Foci | -1.35 | 5.14 | 9.31 | 10.16 | 1.97 | -0.75 | 12.64 | 12.67 | 19.60 | -2.70 | -4.70 | 11.60 | 15.05 | 20.92 | -0.50 |
Eisosomes | 2.03 | 2.17 | 4.35 | 2.91 | 1.37 | 1.52 | 1.01 | -0.70 | -3.49 | -2.25 | 4.09 | 3.63 | 3.62 | -0.06 | -0.44 |
Endoplasmic Reticulum | -0.50 | -7.80 | -3.59 | -3.41 | -0.66 | 2.74 | -2.17 | -2.32 | -5.93 | 1.13 | 1.46 | -4.99 | -5.70 | -6.21 | 0.98 |
Endosome | -3.68 | -2.95 | -8.22 | -5.95 | -3.43 | 0.69 | 0.83 | -4.08 | -6.21 | -4.10 | -4.82 | -3.07 | -12.90 | -9.48 | -5.76 |
Golgi | -0.51 | 0.40 | -0.79 | -0.46 | -0.87 | -1.31 | -5.75 | -3.60 | -3.23 | 3.55 | -0.23 | -3.37 | -2.59 | -2.57 | 1.61 |
Lipid Particles | 2.22 | -0.77 | 5.12 | 3.47 | 3.72 | 1.56 | -0.40 | 0.12 | -1.89 | 0.53 | 5.22 | 0.62 | 6.27 | 1.98 | 3.36 |
Mitochondria | 4.60 | 5.41 | 2.97 | -0.34 | -1.75 | -1.79 | 3.12 | 0.93 | 3.90 | -3.31 | 1.46 | 7.00 | 3.65 | 2.50 | -3.67 |
Mitotic Spindle | -0.99 | -1.06 | -1.90 | -1.51 | -0.10 | 0.69 | -0.00 | 0.24 | -1.75 | 0.29 | -0.42 | -1.21 | -2.15 | -1.87 | 0.33 |
None | 1.45 | -0.28 | -0.40 | -1.46 | -0.08 | 0.45 | -2.68 | -1.25 | -1.51 | 0.86 | 2.76 | -1.02 | 0.22 | -1.48 | 0.89 |
Nuclear Periphery | -1.39 | -4.22 | -5.87 | -3.41 | 1.21 | -0.31 | -1.88 | -4.62 | -4.59 | 1.56 | -1.78 | -2.83 | -8.00 | -4.51 | 1.95 |
Nuclear Periphery Foci | 0.51 | -2.20 | -3.77 | -4.00 | -0.02 | 0.93 | -1.71 | -2.38 | -2.63 | 0.18 | 0.88 | -2.58 | -3.14 | -3.40 | 0.09 |
Nucleolus | 2.71 | 2.60 | 1.11 | -2.31 | -2.16 | 1.32 | -0.50 | -1.06 | -1.30 | -0.92 | 3.14 | 2.26 | -0.20 | -1.44 | -1.14 |
Nucleus | 1.41 | -0.31 | 0.43 | -1.65 | 1.03 | 1.93 | -0.88 | -0.52 | -2.28 | 0.37 | 1.78 | -0.05 | 0.70 | -2.33 | 1.30 |
Peroxisomes | 3.77 | 8.04 | 5.74 | 2.89 | -1.61 | -1.88 | 2.69 | 3.43 | 5.19 | -0.25 | 3.20 | 7.97 | 7.66 | 6.50 | -1.16 |
Vacuole | -0.99 | -4.88 | -7.28 | -7.18 | -4.86 | 2.79 | -2.62 | -3.88 | -7.48 | -0.53 | 0.92 | -6.15 | -10.04 | -10.47 | -3.52 |
Vacuole Periphery | -2.57 | -4.96 | -8.25 | -7.44 | -4.83 | 1.71 | -3.12 | -3.51 | -7.71 | 0.47 | -1.64 | -7.09 | -11.78 | -10.84 | -2.74 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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