Standard name | |
---|---|
Human Ortholog | |
Description | Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 11 | 8 | 9 | 4 | 4 | 6 | 4 | 3 | 7 | 6 | 10 | 29 | 35 | 24 | 10 | 12 | 8 | 3 | 5 | 7 | 5 | 8 | 13 |
Bud | 0 | 0 | 3 | 7 | 3 | 29 | 12 | 17 | 28 | 19 | 40 | 30 | 2 | 2 | 2 | 0 | 4 | 3 | 1 | 0 | 1 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 8 | 4 | 14 | 16 | 12 | 16 | 14 | 22 | 24 | 20 | 24 | 5 | 3 | 8 | 10 | 11 | 13 | 0 | 0 | 1 | 2 | 2 | 3 |
Cytoplasm | 56 | 93 | 85 | 162 | 200 | 243 | 254 | 314 | 313 | 269 | 224 | 280 | 91 | 76 | 148 | 65 | 73 | 61 | 12 | 23 | 17 | 21 | 35 | 45 |
Endoplasmic Reticulum | 196 | 225 | 203 | 114 | 224 | 152 | 258 | 128 | 151 | 177 | 97 | 193 | 229 | 237 | 215 | 2 | 3 | 2 | 327 | 321 | 358 | 288 | 340 | 356 |
Endosome | 4 | 2 | 11 | 21 | 21 | 12 | 7 | 41 | 17 | 16 | 16 | 9 | 11 | 27 | 45 | 5 | 16 | 8 | 1 | 1 | 1 | 2 | 3 | 3 |
Golgi | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 0 | 3 | 4 | 0 | 1 | 1 | 4 | 3 | 2 |
Mitochondria | 8 | 1 | 51 | 109 | 174 | 313 | 490 | 262 | 353 | 366 | 358 | 560 | 0 | 0 | 4 | 10 | 16 | 9 | 7 | 6 | 7 | 26 | 30 | 27 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 8 | 13 | 17 | 8 | 9 | 8 | 11 | 1 | 2 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 2 | 5 | 4 | 6 | 17 | 38 | 18 | 10 | 34 | 25 | 21 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 2 | 0 | 1 | 10 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 4 | 6 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 62 | 33 | 49 | 125 | 97 | 147 | 197 | 206 | 199 | 276 | 129 | 150 | 8 | 11 | 38 | 22 | 68 | 47 | 2 | 6 | 3 | 14 | 16 | 28 |
Unique Cell Count | 299 | 326 | 353 | 471 | 635 | 736 | 1048 | 865 | 861 | 968 | 715 | 1052 | 339 | 343 | 450 | 116 | 186 | 144 | 362 | 373 | 407 | 376 | 452 | 503 |
Labelled Cell Count | 339 | 376 | 420 | 567 | 750 | 941 | 1296 | 1026 | 1109 | 1202 | 928 | 1299 | 379 | 402 | 497 | 128 | 211 | 158 | 362 | 373 | 407 | 376 | 452 | 503 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 8.9 | 7.2 | 7.0 | 6.2 | 5.9 | 5.4 | 6.0 | 5.7 | 5.5 | 5.5 | 5.6 | 8.6 | 8.3 | 8.9 | 8.4 | 8.8 | 8.7 | 9.5 | 9.4 | 9.8 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.4 | 1.6 | 1.4 | 1.4 | 1.1 | 1.3 | 1.3 | 1.2 | 1.4 | 1.3 | 1.3 | 1.2 | 1.4 | 1.4 | 1.6 | 1.6 | 1.6 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.21 | -0.29 | -0.41 | -0.26 | -0.33 | -0.37 | -0.38 | -0.36 | 0.27 | 0.21 | 0.31 | 0.23 | 0.29 | 0.28 | 0.41 | 0.39 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 4.3 | 2.2 | 0 | 2.4 | 0 |
Bud | 0 | 0 | 2.8 | 0 | 1.4 | 2.4 | 1.4 | 3.7 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | 1.5 | 0.6 | 0.5 | 0.6 | 1.6 | 1.5 | 1.7 | 1.3 | 0 | 0 | 0.7 | 0 | 3.2 | 0 |
Cytoplasm | 3.2 | 2.5 | 3.0 | 0.1 | 4.1 | 4.1 | 1.3 | 2.5 | 0.9 | 0.8 | -0.6 | 2.7 | 6.4 | 3.7 | 4.1 |
Endoplasmic Reticulum | -9.7 | -6.8 | -12.1 | -11.4 | -15.2 | -13.9 | -13.9 | -15.0 | -14.2 | 2.7 | 3.2 | -2.7 | -10.5 | -12.7 | -11.5 |
Endosome | 1.0 | 0.2 | -1.6 | 0 | 1.3 | -1.2 | -1.7 | -0.9 | -3.1 | 0.1 | 2.8 | 3.8 | 0 | 2.8 | 1.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 4.7 | 9.2 | 10.8 | 5.7 | 8.9 | 8.1 | 11.3 | 12.7 | -7.3 | -7.3 | -7.5 | -1.6 | -2.0 | -2.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1.0 | 2.1 | 0.8 | 0 | 2.0 | 1.9 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.4 | 0.6 | 2.4 | 2.1 | 3.9 | 3.6 | 5.5 | 1.7 | 0.2 | -5.5 | -5.0 | -2.5 | 1.3 | 6.1 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8026 | 4.1719 | 3.9596 | 3.3406 | 3.4454 | 3.9029 | 5.2192 | 6.1034 | 5.5089 | 4.9187 | 4.8442 | 5.3474 | 3.0139 | 3.4651 | 3.0347 | 3.4493 | 2.8607 | 3.2047 |
Actin | 0.012 | 0.0026 | 0.0167 | 0.0187 | 0.0017 | 0.0048 | 0.0064 | 0.0008 | 0.0106 | 0.0038 | 0.0049 | 0.0076 | 0.011 | 0.0028 | 0.0122 | 0.0043 | 0.0085 | 0.0018 |
Bud | 0.0044 | 0.0108 | 0.0146 | 0.0027 | 0.0002 | 0.0058 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 |
Bud Neck | 0.0087 | 0.0004 | 0.0017 | 0.0009 | 0.0005 | 0.0037 | 0.0011 | 0.0001 | 0.0004 | 0.0003 | 0.0025 | 0.0009 | 0.0011 | 0.0003 | 0.0014 | 0.0003 | 0.0011 | 0.0003 |
Bud Periphery | 0.0026 | 0.0016 | 0.0037 | 0.0011 | 0.0002 | 0.0024 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0001 |
Bud Site | 0.0087 | 0.0308 | 0.0134 | 0.001 | 0.0005 | 0.0012 | 0.0018 | 0.0006 | 0.0014 | 0.0001 | 0.0006 | 0.0001 | 0.0022 | 0.001 | 0.0011 | 0.0005 | 0.0002 | 0.0001 |
Cell Periphery | 0.0015 | 0.001 | 0.0017 | 0.0003 | 0.0002 | 0.0004 | 0.0009 | 0.0009 | 0.001 | 0.0005 | 0.0012 | 0.0003 | 0.0006 | 0.0006 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Cytoplasm | 0.1263 | 0.2143 | 0.1411 | 0.1194 | 0.1504 | 0.2205 | 0.1095 | 0.1626 | 0.1648 | 0.0957 | 0.1644 | 0.1535 | 0.1062 | 0.175 | 0.1476 | 0.1206 | 0.0478 | 0.1333 |
Cytoplasmic Foci | 0.0356 | 0.0164 | 0.0223 | 0.0269 | 0.0303 | 0.0227 | 0.017 | 0.0109 | 0.0128 | 0.005 | 0.0074 | 0.016 | 0.0205 | 0.0095 | 0.012 | 0.0052 | 0.0067 | 0.0048 |
Eisosomes | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.4992 | 0.5746 | 0.6061 | 0.5886 | 0.5593 | 0.561 | 0.7349 | 0.6908 | 0.6871 | 0.8182 | 0.7573 | 0.7253 | 0.7088 | 0.7362 | 0.7299 | 0.7544 | 0.8148 | 0.7736 |
Endosome | 0.0871 | 0.0635 | 0.0466 | 0.0785 | 0.0751 | 0.0563 | 0.025 | 0.0289 | 0.0321 | 0.0315 | 0.0209 | 0.034 | 0.052 | 0.0282 | 0.0293 | 0.0498 | 0.0249 | 0.027 |
Golgi | 0.0259 | 0.0054 | 0.0151 | 0.0248 | 0.0074 | 0.0063 | 0.0042 | 0.005 | 0.0086 | 0.0065 | 0.0022 | 0.0035 | 0.0094 | 0.0032 | 0.0069 | 0.0066 | 0.0082 | 0.0034 |
Lipid Particles | 0.0249 | 0.0069 | 0.0166 | 0.0198 | 0.0094 | 0.0071 | 0.0131 | 0.019 | 0.0143 | 0.0033 | 0.0054 | 0.0085 | 0.0188 | 0.0079 | 0.0071 | 0.0034 | 0.0305 | 0.0031 |
Mitochondria | 0.0246 | 0.0018 | 0.0183 | 0.0142 | 0.0814 | 0.0063 | 0.0035 | 0.003 | 0.0013 | 0.0016 | 0.0005 | 0.0009 | 0.0161 | 0.0009 | 0.0022 | 0.002 | 0.0019 | 0.0019 |
None | 0.0416 | 0.0233 | 0.0198 | 0.0218 | 0.0047 | 0.0388 | 0.0549 | 0.0436 | 0.032 | 0.0089 | 0.01 | 0.0247 | 0.0111 | 0.0066 | 0.0206 | 0.02 | 0.0011 | 0.0098 |
Nuclear Periphery | 0.0315 | 0.0207 | 0.0185 | 0.0235 | 0.026 | 0.0271 | 0.0136 | 0.0157 | 0.0166 | 0.017 | 0.0109 | 0.0141 | 0.0207 | 0.0179 | 0.0199 | 0.0211 | 0.0362 | 0.0299 |
Nucleolus | 0.003 | 0.0005 | 0.0035 | 0.0003 | 0.0007 | 0.0013 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 |
Nucleus | 0.0083 | 0.0072 | 0.0114 | 0.0063 | 0.0296 | 0.0119 | 0.0019 | 0.0069 | 0.0046 | 0.0022 | 0.0026 | 0.0026 | 0.0021 | 0.0021 | 0.0018 | 0.0026 | 0.0039 | 0.0035 |
Peroxisomes | 0.0222 | 0.0008 | 0.0104 | 0.0405 | 0.0068 | 0.003 | 0.0042 | 0.0038 | 0.0017 | 0.0001 | 0.0003 | 0.0011 | 0.0051 | 0.0004 | 0.0012 | 0.0003 | 0.0029 | 0.0025 |
Punctate Nuclear | 0.0082 | 0.0046 | 0.0098 | 0.0016 | 0.0034 | 0.0107 | 0.0033 | 0.0008 | 0.0035 | 0.0003 | 0.0006 | 0.0019 | 0.0012 | 0.0012 | 0.0006 | 0.001 | 0.0049 | 0.0008 |
Vacuole | 0.0184 | 0.0109 | 0.006 | 0.0065 | 0.0078 | 0.0068 | 0.0031 | 0.0052 | 0.0054 | 0.0038 | 0.0071 | 0.004 | 0.0088 | 0.005 | 0.0045 | 0.0052 | 0.004 | 0.003 |
Vacuole Periphery | 0.0051 | 0.0017 | 0.0023 | 0.0022 | 0.0045 | 0.0019 | 0.0008 | 0.0007 | 0.0011 | 0.001 | 0.0005 | 0.0008 | 0.0029 | 0.0009 | 0.0008 | 0.0012 | 0.0016 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.1499 | 35.8181 | 36.1196 | 35.8127 | 34.6331 | 42.0992 | 45.0374 | 36.038 | 40.8946 | 44.7342 |
Translational Efficiency | 0.6035 | 0.5782 | 0.6475 | 0.6433 | 0.652 | 0.6998 | 0.5753 | 0.5688 | 0.6236 | 0.5691 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
363 | 1395 | 186 | 1698 | 1794 | 1999 | 2467 | 1443 | 2157 | 3394 | 2653 | 3141 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 746.81 | 873.78 | 1248.43 | 1219.48 | 816.18 | 857.00 | 1167.65 | 1230.71 | 804.51 | 863.90 | 1173.31 | 1224.64 |
Standard Deviation | 96.53 | 153.26 | 150.54 | 198.81 | 119.08 | 128.86 | 158.73 | 192.17 | 118.47 | 139.65 | 159.51 | 195.87 |
Intensity Change Log 2 | — | 0.226529 | 0.741302 | 0.707453 | — | 0.070408 | 0.516649 | 0.592532 | — | 0.147114 | 0.628360 | 0.648585 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000573 | 0.000715 | 0.002303 | 0.004307 | 0.000405 | 0.000640 | 0.000671 | 0.003948 | 0.000434 | 0.000671 | 0.000785 | 0.004142 |
Bud Neck | 0.002581 | 0.001848 | 0.001995 | 0.009556 | 0.001544 | 0.001929 | 0.000979 | 0.006048 | 0.001719 | 0.001896 | 0.001050 | 0.007945 |
Bud Site | 0.005096 | 0.006108 | 0.006512 | 0.029967 | 0.002996 | 0.004076 | 0.003113 | 0.025548 | 0.003350 | 0.004911 | 0.003351 | 0.027937 |
Cell Periphery | 0.000793 | 0.000416 | 0.000387 | 0.001498 | 0.000408 | 0.000489 | 0.000855 | 0.002608 | 0.000473 | 0.000459 | 0.000822 | 0.002008 |
Cytoplasm | 0.182828* | 0.157854* | 0.025502 | 0.143183* | 0.168373* | 0.135072* | 0.088555 | 0.110641* | 0.170805* | 0.144436* | 0.084135 | 0.128233* |
Cytoplasmic Foci | 0.060017 | 0.064955 | 0.022356 | 0.028527 | 0.071792 | 0.060082 | 0.003536 | 0.022798 | 0.069811 | 0.062085 | 0.004855 | 0.025895 |
Eisosomes | 0.000653 | 0.000160 | 0.000038 | 0.000245 | 0.000312 | 0.000329 | 0.000105 | 0.000383 | 0.000369 | 0.000259 | 0.000101 | 0.000308 |
Endoplasmic Reticulum | 0.540528* | 0.580794* | 0.560657* | 0.468420* | 0.570160* | 0.623688* | 0.856397* | 0.634904* | 0.565173* | 0.606058* | 0.835663* | 0.544904* |
Endosome | 0.009245 | 0.018073 | 0.041733 | 0.024932 | 0.014316 | 0.016075 | 0.004332 | 0.020130 | 0.013462 | 0.016896 | 0.006954 | 0.022726 |
Golgi | 0.023312 | 0.014369 | 0.002662 | 0.007771 | 0.021725 | 0.024413 | 0.001639 | 0.008201 | 0.021992 | 0.020284 | 0.001711 | 0.007969 |
Lipid Particles | 0.100482* | 0.049580 | 0.034627 | 0.040155 | 0.069100 | 0.062110 | 0.007282 | 0.036245 | 0.074382 | 0.056960 | 0.009199 | 0.038359 |
Mitochondria | 0.007726 | 0.007062 | 0.000102 | 0.004626 | 0.009600 | 0.011919 | 0.000823 | 0.002942 | 0.009285 | 0.009923 | 0.000772 | 0.003853 |
Mitotic Spindle | 0.000419 | 0.001861 | 0.003569 | 0.020173 | 0.000476 | 0.001101 | 0.000235 | 0.006575 | 0.000467 | 0.001413 | 0.000469 | 0.013926 |
None | 0.003755 | 0.005646 | 0.000040 | 0.002486 | 0.004742 | 0.004254 | 0.002754 | 0.001560 | 0.004576 | 0.004826 | 0.002564 | 0.002061 |
Nuclear Periphery | 0.006162 | 0.007106 | 0.042892 | 0.016458 | 0.007074 | 0.004004 | 0.001914 | 0.003456 | 0.006920 | 0.005279 | 0.004787 | 0.010485 |
Nuclear Periphery Foci | 0.009030 | 0.019995 | 0.171304 | 0.062625 | 0.011229 | 0.009021 | 0.010851 | 0.037612 | 0.010859 | 0.013532 | 0.022100 | 0.051134 |
Nucleolus | 0.000381 | 0.000648 | 0.000110 | 0.000397 | 0.001023 | 0.000255 | 0.000040 | 0.000309 | 0.000915 | 0.000417 | 0.000045 | 0.000356 |
Nucleus | 0.019280 | 0.018497 | 0.003585 | 0.028387 | 0.018265 | 0.015194 | 0.007313 | 0.014410 | 0.018436 | 0.016552 | 0.007051 | 0.021966 |
Peroxisomes | 0.003724 | 0.001797 | 0.000730 | 0.001008 | 0.002083 | 0.002861 | 0.000180 | 0.000980 | 0.002359 | 0.002423 | 0.000218 | 0.000995 |
Vacuole | 0.019994 | 0.038220 | 0.077098 | 0.100550 | 0.020845 | 0.019079 | 0.008072 | 0.058763 | 0.020701 | 0.026947 | 0.012911 | 0.081353 |
Vacuole Periphery | 0.003424 | 0.004296 | 0.001798 | 0.004729 | 0.003530 | 0.003409 | 0.000354 | 0.001935 | 0.003512 | 0.003773 | 0.000455 | 0.003446 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.49 | -1.93 | -6.33 | -6.29 | -1.86 | -2.01 | -1.37 | -7.00 | -6.57 | -6.14 | -2.02 | -1.82 | -9.80 | -9.14 | -8.02 |
Bud Neck | 1.14 | 0.81 | -8.63 | -11.03 | -10.51 | -1.04 | 1.56 | -6.55 | -6.03 | -7.42 | -0.57 | 2.06 | -12.35 | -12.35 | -14.01 |
Bud Site | -0.35 | -0.48 | -6.96 | -9.45 | -6.93 | -1.33 | -0.42 | -9.77 | -9.37 | -9.68 | -1.82 | -0.27 | -13.99 | -13.54 | -14.25 |
Cell Periphery | 3.19 | 2.98 | -3.45 | -6.56 | -6.25 | -1.30 | -3.47 | -6.54 | -6.30 | -4.83 | 0.25 | -2.95 | -8.41 | -8.62 | -5.38 |
Cytoplasm | 2.02 | 13.47 | 2.55 | 0.76 | -18.42 | 5.41 | 14.31 | 7.30 | 2.70 | -4.84 | 4.81 | 16.86 | 6.08 | 1.66 | -11.44 |
Cytoplasmic Foci | -0.72 | 5.13 | 5.11 | 9.95 | -1.35 | 3.04 | 23.48 | 14.49 | 11.79 | -11.97 | 2.37 | 24.67 | 15.13 | 15.14 | -17.34 |
Eisosomes | 5.64 | 7.12 | 4.60 | -2.48 | -7.01 | -0.26 | 3.03 | -0.83 | -0.82 | -4.32 | 1.96 | 4.50 | 1.07 | -1.28 | -5.02 |
Endoplasmic Reticulum | -1.82 | -0.51 | 5.78 | 11.69 | 5.33 | -4.58 | -29.74 | -2.52 | 1.54 | 22.55 | -4.03 | -29.72 | 5.85 | 10.73 | 38.89* |
Endosome | -6.72 | -7.20 | -10.52 | -4.56 | 3.82 | -1.46 | 12.69 | -4.18 | -2.68 | -12.81 | -3.73 | 8.34 | -8.86 | -5.37 | -16.69 |
Golgi | 2.60 | 6.14 | 4.76 | 4.99 | -3.43 | -1.38 | 15.03 | 8.29 | 9.54 | -5.91 | 1.12 | 16.30 | 10.22 | 10.86 | -7.48 |
Lipid Particles | 6.10 | 6.69 | 7.20 | 2.39 | -1.05 | 1.83 | 21.62 | 8.62 | 6.81 | -11.52 | 5.33 | 23.34 | 11.21 | 6.93 | -16.29 |
Mitochondria | 0.30 | 3.67 | 1.58 | 2.57 | -5.65 | -1.42 | 7.82 | 5.30 | 6.80 | -3.16 | -0.50 | 8.54 | 5.14 | 6.91 | -5.69 |
Mitotic Spindle | -1.70 | -2.95 | -9.46 | -8.41 | -7.38 | -1.30 | 1.64 | -5.02 | -4.31 | -5.19 | -2.14 | -0.15 | -10.66 | -9.59 | -10.64 |
None | -1.14 | 2.85 | 1.06 | 2.91 | -3.88 | 0.44 | 2.02 | 2.84 | 2.86 | 1.48 | -0.26 | 2.32 | 2.99 | 4.05 | 1.07 |
Nuclear Periphery | -0.75 | -6.05 | -5.83 | -5.51 | 4.39 | 3.44 | 6.22 | 3.79 | 0.76 | -2.80 | 2.07 | 2.52 | -3.14 | -5.54 | -5.77 |
Nuclear Periphery Foci | -5.07 | -12.66 | -16.36 | -12.82 | 8.23 | 1.70 | 0.19 | -10.25 | -11.32 | -10.64 | -2.31 | -7.00 | -19.04 | -17.89 | -12.31 |
Nucleolus | -1.62 | 2.16 | -0.36 | 1.87 | -5.28 | 2.82 | 3.58 | 2.40 | -0.37 | -1.95 | 2.17 | 3.79 | 2.30 | 0.53 | -5.02 |
Nucleus | 0.26 | 8.41 | -5.55 | -6.28 | -14.54 | 2.19 | 9.29 | 0.87 | -1.00 | -6.65 | 1.51 | 10.82 | -4.88 | -6.35 | -14.23 |
Peroxisomes | 3.45 | 4.79 | 4.92 | 4.62 | -0.68 | -2.95 | 12.29 | 5.24 | 7.24 | -5.29 | -0.25 | 13.28 | 7.66 | 8.74 | -7.85 |
Vacuole | -5.37 | -5.65 | -20.17 | -16.79 | -4.66 | 1.01 | 8.58 | -12.94 | -13.35 | -16.27 | -4.00 | 4.71 | -24.66 | -22.35 | -26.82 |
Vacuole Periphery | -0.83 | 1.55 | -0.72 | 0.06 | -3.40 | 0.34 | 11.14 | 4.20 | 4.04 | -4.71 | -0.74 | 10.42 | 0.80 | 1.49 | -7.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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