Standard name | |
---|---|
Human Ortholog | |
Description | Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 7 | 3 | 11 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 1 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 6 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 4 | 1 | 2 | 4 | 2 | 0 | 0 | 0 | 7 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 120 | 130 | 85 | 115 | 124 | 104 | 121 | 132 | 66 | 82 | 68 | 79 | 66 | 106 | 94 | 145 | 96 | 129 | 124 | 135 | 86 | 45 | 71 | 90 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 3 | 0 | 0 | 0 | 5 | 0 | 4 | 0 | 1 | 1 | 0 | 2 | 1 |
Endosome | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 5 | 1 | 17 | 24 | 23 | 31 | 28 | 48 | 20 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleus | 65 | 67 | 72 | 103 | 126 | 120 | 141 | 165 | 90 | 120 | 137 | 98 | 90 | 87 | 82 | 86 | 48 | 49 | 60 | 67 | 77 | 52 | 100 | 38 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 11 | 10 | 13 | 9 | 10 | 37 | 65 | 72 | 46 | 68 | 98 | 60 | 5 | 7 | 12 | 10 | 7 | 14 | 4 | 3 | 4 | 28 | 42 | 27 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 6 | 1 |
Vac/Vac Membrane | 2 | 5 | 11 | 29 | 36 | 44 | 59 | 90 | 38 | 69 | 63 | 61 | 9 | 8 | 6 | 41 | 15 | 18 | 0 | 3 | 3 | 10 | 25 | 11 |
Unique Cell Count | 191 | 201 | 171 | 241 | 285 | 286 | 371 | 444 | 238 | 324 | 338 | 279 | 162 | 200 | 194 | 274 | 161 | 202 | 198 | 217 | 177 | 142 | 264 | 184 |
Labelled Cell Count | 199 | 212 | 183 | 262 | 300 | 326 | 422 | 492 | 284 | 382 | 438 | 332 | 171 | 208 | 199 | 301 | 171 | 222 | 198 | 217 | 177 | 142 | 264 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.1 | 12.3 | 11.1 | 11.0 | 12.2 | 11.4 | 11.4 | 12.2 | 11.0 | 11.6 | 11.3 | 12.9 | 15.0 | 16.1 | 17.0 | 23.9 | 23.0 | 21.8 | 17.8 | 16.7 | 16.7 |
Std Deviation (1e-4) | 4.6 | 5.9 | 4.2 | 4.7 | 4.3 | 3.9 | 4.0 | 3.8 | 3.6 | 4.1 | 4.0 | 4.2 | 6.6 | 5.9 | 6.8 | 8.6 | 7.8 | 7.4 | 8.7 | 7.8 | 8.2 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.15 | 0.04 | 0.04 | 0.14 | -0.01 | 0.07 | 0.02 | 0.22 | 0.44 | 0.54 | 0.62 | 1.11 | 1.05 | 0.98 | 0.68 | 0.6 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -1.3 | -2.8 | -3.8 | -4.6 | -4.5 | -5.5 | -6.9 | -4.6 | -1.6 | 0.6 | -0.2 | 0.7 | 1.8 | 2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.9 | 3.0 | 2.6 | 4.6 | 3.6 | 4.9 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.1 | 0.4 | 0 | -0.9 | -1.1 | -0.9 | -1.1 | -0.3 | -1.5 | 2.5 | 0.3 | 0 | -2.3 | -2.3 | -3.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.7 | -1.9 | 1.8 | 3.1 | 2.8 | 3.3 | 3.8 | 5.5 | 3.9 | -1.8 | -1.7 | -0.5 | -1.8 | -1.2 | -0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.9 | 2.1 | 2.8 | 3.1 | 4.1 | 2.9 | 4.3 | 3.7 | 4.3 | -0.3 | -1.1 | -1.5 | 2.7 | 1.0 | 0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.7206 | 10.8584 | 10.0135 | 9.7113 | 8.3877 | 10.2071 | 11.8167 | 10.6095 | 10.0916 | 9.6849 | 8.8113 | 9.6025 | 12.8194 | 10.8602 | 10.5845 | 9.6963 | 9.2304 | 10.919 |
Actin | 0.0174 | 0 | 0.0249 | 0.0016 | 0.0149 | 0.0023 | 0.0535 | 0 | 0.0087 | 0.004 | 0.001 | 0.0041 | 0.0184 | 0 | 0.0004 | 0.0021 | 0.0086 | 0 |
Bud | 0.0008 | 0 | 0.0008 | 0.0001 | 0.0025 | 0.0002 | 0.0005 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0014 | 0.0007 | 0.0023 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 |
Bud Periphery | 0.0015 | 0.0001 | 0.0013 | 0.0003 | 0.0022 | 0.0004 | 0.0007 | 0 | 0 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0003 | 0.0006 | 0.0004 | 0.0001 |
Bud Site | 0.0026 | 0 | 0.0025 | 0.0003 | 0.0246 | 0.0002 | 0.0033 | 0 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0021 | 0 | 0.0003 | 0.0007 | 0.0002 | 0 |
Cell Periphery | 0.0008 | 0 | 0.0006 | 0.0002 | 0.0007 | 0.0003 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0002 | 0 |
Cytoplasm | 0.008 | 0 | 0.004 | 0.001 | 0.0049 | 0.0023 | 0.0028 | 0 | 0.0013 | 0.0006 | 0.0072 | 0.0003 | 0.0007 | 0 | 0.0015 | 0.0003 | 0.0003 | 0 |
Cytoplasmic Foci | 0.0263 | 0 | 0.0203 | 0.0077 | 0.0389 | 0.0063 | 0.0053 | 0 | 0.0082 | 0.0099 | 0.0154 | 0.0022 | 0.0039 | 0 | 0.002 | 0.0099 | 0.0032 | 0 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0024 | 0 | 0.0012 | 0.0021 | 0.0016 | 0.001 | 0.0028 | 0 | 0.0009 | 0.0005 | 0.0011 | 0.0013 | 0.0008 | 0 | 0.0008 | 0.0003 | 0.0003 | 0 |
Endosome | 0.0206 | 0.0001 | 0.0127 | 0.0157 | 0.0295 | 0.0128 | 0.0084 | 0 | 0.0039 | 0.0129 | 0.0164 | 0.0071 | 0.0041 | 0 | 0.003 | 0.0032 | 0.0041 | 0 |
Golgi | 0.0071 | 0 | 0.0086 | 0.003 | 0.0174 | 0.0037 | 0.0048 | 0 | 0.0027 | 0.0057 | 0.0063 | 0.0019 | 0.0033 | 0 | 0.0004 | 0.003 | 0.0047 | 0 |
Lipid Particles | 0.009 | 0 | 0.0184 | 0.0097 | 0.0877 | 0.0087 | 0.0058 | 0 | 0.0018 | 0.0041 | 0.0426 | 0.0051 | 0.0021 | 0 | 0.002 | 0.0059 | 0.0482 | 0 |
Mitochondria | 0.0118 | 0.0002 | 0.0239 | 0.0153 | 0.0443 | 0.0029 | 0.0037 | 0.0001 | 0.0004 | 0.0011 | 0.0106 | 0.013 | 0.0021 | 0.0002 | 0.0006 | 0.0043 | 0.0043 | 0.0002 |
None | 0.0064 | 0 | 0.0022 | 0.0017 | 0.0021 | 0.0007 | 0.0019 | 0 | 0.0049 | 0.0003 | 0.0017 | 0.0006 | 0.0144 | 0 | 0.0008 | 0.0004 | 0.0022 | 0 |
Nuclear Periphery | 0.008 | 0.001 | 0.005 | 0.0041 | 0.0079 | 0.0022 | 0.0044 | 0.0004 | 0.0066 | 0.0028 | 0.0092 | 0.0149 | 0.003 | 0.0003 | 0.0044 | 0.0026 | 0.019 | 0.0002 |
Nucleolus | 0.0491 | 0.0171 | 0.025 | 0.029 | 0.1972 | 0.1428 | 0.0205 | 0.0157 | 0.0178 | 0.0083 | 0.1512 | 0.0892 | 0.016 | 0.0215 | 0.0157 | 0.0444 | 0.3089 | 0.0602 |
Nucleus | 0.7836 | 0.9806 | 0.8278 | 0.8935 | 0.4635 | 0.8025 | 0.87 | 0.9835 | 0.9353 | 0.9366 | 0.6947 | 0.8444 | 0.9227 | 0.9779 | 0.9637 | 0.8985 | 0.5609 | 0.9387 |
Peroxisomes | 0.0053 | 0 | 0.0065 | 0.0037 | 0.0245 | 0.0027 | 0.001 | 0 | 0.0013 | 0.0031 | 0.0131 | 0.0081 | 0.002 | 0 | 0.0003 | 0.0162 | 0.0136 | 0 |
Punctate Nuclear | 0.0252 | 0.0002 | 0.0053 | 0.0019 | 0.0213 | 0.0042 | 0.0057 | 0.0001 | 0.0053 | 0.0079 | 0.0275 | 0.0054 | 0.0015 | 0.0001 | 0.0026 | 0.0008 | 0.0179 | 0.0001 |
Vacuole | 0.0059 | 0.0004 | 0.004 | 0.0032 | 0.006 | 0.0017 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0 | 0.0005 | 0.003 | 0.0006 | 0.0001 |
Vacuole Periphery | 0.0075 | 0.0002 | 0.0042 | 0.0059 | 0.0066 | 0.0014 | 0.0008 | 0 | 0.0002 | 0.0003 | 0.0006 | 0.0009 | 0.0004 | 0 | 0.0003 | 0.0028 | 0.0018 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.8692 | 39.2309 | 34.656 | 35.1089 | 44.7066 | 38.6395 | 36.833 | 41.1143 | 46.2499 | 43.8858 |
Translational Efficiency | 3.2708 | 2.919 | 2.6633 | 3.1889 | 2.8688 | 2.9086 | 2.5291 | 2.8201 | 2.4145 | 2.5757 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1199 | 840 | 3252 | 1600 | 1479 | 52 | 125 | 1103 | 2678 | 892 | 3377 | 2703 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1098.59 | 1366.74 | 1565.68 | 1626.65 | 1373.38 | 1479.32 | 1792.38 | 1830.97 | 1250.35 | 1373.30 | 1574.07 | 1710.03 |
Standard Deviation | 331.60 | 366.15 | 743.37 | 449.39 | 311.50 | 314.86 | 257.64 | 410.64 | 348.55 | 364.31 | 732.41 | 445.46 |
Intensity Change Log 2 | — | 0.315086 | 0.511136 | 0.566251 | — | 0.107203 | 0.384146 | 0.414877 | — | 0.203305 | 0.441967 | 0.484118 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000596 | 0.000508 | 0.000513 | 0.000532 | 0.000507 | 0.000712 | 0.000858 | 0.000677 | 0.000546 | 0.000520 | 0.000526 | 0.000591 |
Bud Neck | 0.001256 | 0.002841 | 0.010584 | 0.005702 | 0.002261 | 0.002819 | 0.007489 | 0.011434 | 0.001811 | 0.002839 | 0.010470 | 0.008041 |
Bud Site | 0.001583 | 0.000564 | 0.000693 | 0.000550 | 0.000562 | 0.000293 | 0.000749 | 0.002187 | 0.001019 | 0.000548 | 0.000695 | 0.001218 |
Cell Periphery | 0.000239 | 0.000142 | 0.000229 | 0.000149 | 0.000077 | 0.000020 | 0.000024 | 0.000117 | 0.000149 | 0.000135 | 0.000221 | 0.000136 |
Cytoplasm | 0.002135 | 0.000436 | 0.001451 | 0.000566 | 0.000093 | 0.000020 | 0.000047 | 0.000601 | 0.001007 | 0.000412 | 0.001399 | 0.000580 |
Cytoplasmic Foci | 0.000220 | 0.000122 | 0.000405 | 0.000039 | 0.000266 | 0.000059 | 0.000680 | 0.000948 | 0.000245 | 0.000119 | 0.000416 | 0.000410 |
Eisosomes | 0.000187 | 0.000120 | 0.000115 | 0.000041 | 0.000210 | 0.000115 | 0.000148 | 0.000045 | 0.000200 | 0.000120 | 0.000116 | 0.000043 |
Endoplasmic Reticulum | 0.000894 | 0.000543 | 0.000930 | 0.000472 | 0.000674 | 0.001199 | 0.000631 | 0.000440 | 0.000772 | 0.000581 | 0.000919 | 0.000459 |
Endosome | 0.000057 | 0.000061 | 0.000114 | 0.000028 | 0.000054 | 0.000016 | 0.001069 | 0.001551 | 0.000055 | 0.000058 | 0.000150 | 0.000650 |
Golgi | 0.000132 | 0.000143 | 0.000104 | 0.000029 | 0.000225 | 0.000038 | 0.000225 | 0.001135 | 0.000183 | 0.000137 | 0.000109 | 0.000481 |
Lipid Particles | 0.000046 | 0.000027 | 0.000009 | 0.000004 | 0.000012 | 0.000020 | 0.000053 | 0.000049 | 0.000027 | 0.000027 | 0.000010 | 0.000022 |
Mitochondria | 0.000794 | 0.001894 | 0.001372 | 0.001738 | 0.000670 | 0.001369 | 0.002072 | 0.003000 | 0.000726 | 0.001863 | 0.001398 | 0.002253 |
Mitotic Spindle | 0.000021 | 0.000039 | 0.000441 | 0.001400 | 0.000038 | 0.000016 | 0.001159 | 0.005332 | 0.000030 | 0.000037 | 0.000467 | 0.003004 |
None | 0.007752 | 0.003453 | 0.003110 | 0.000866 | 0.000464 | 0.000604 | 0.000376 | 0.001027 | 0.003727 | 0.003287 | 0.003009 | 0.000932 |
Nuclear Periphery | 0.000036 | 0.000026 | 0.000284 | 0.000291 | 0.000015 | 0.000108 | 0.000321 | 0.000559 | 0.000025 | 0.000031 | 0.000286 | 0.000400 |
Nuclear Periphery Foci | 0.000403 | 0.000091 | 0.000042 | 0.000070 | 0.000058 | 0.000020 | 0.000345 | 0.000185 | 0.000212 | 0.000087 | 0.000053 | 0.000117 |
Nucleolus | 0.026226 | 0.021958 | 0.010357 | 0.012100 | 0.027220 | 0.048777 | 0.019912 | 0.016649 | 0.026775 | 0.023522 | 0.010710 | 0.013956 |
Nucleus | 0.954331* | 0.965040* | 0.968256* | 0.974480* | 0.963933* | 0.936613* | 0.955557* | 0.949201* | 0.959634* | 0.963383* | 0.967786* | 0.964165* |
Peroxisomes | 0.001632 | 0.001359 | 0.000243 | 0.000506 | 0.001781 | 0.005069 | 0.003369 | 0.000839 | 0.001714 | 0.001575 | 0.000358 | 0.000642 |
Vacuole | 0.001084 | 0.000424 | 0.000443 | 0.000285 | 0.000641 | 0.000550 | 0.002119 | 0.002305 | 0.000840 | 0.000431 | 0.000505 | 0.001109 |
Vacuole Periphery | 0.000380 | 0.000208 | 0.000307 | 0.000152 | 0.000238 | 0.001563 | 0.002794 | 0.001719 | 0.000301 | 0.000287 | 0.000399 | 0.000791 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.13 | 0.97 | 0.73 | -0.67 | -0.38 | -1.96 | -4.06 | -2.97 | -0.32 | 1.94 | 0.53 | 0.20 | -1.31 | -2.00 | -2.15 |
Bud Neck | -5.73 | -14.55 | -10.33 | -6.19 | 5.90 | -0.34 | -8.14 | -10.76 | -5.37 | -4.31 | -3.58 | -13.96 | -14.25 | -10.91 | 2.51 |
Bud Site | 1.42 | 1.21 | 1.31 | -0.21 | 0.49 | 1.35 | -0.65 | -2.29 | -2.73 | -2.16 | 1.39 | 0.91 | -0.72 | -2.28 | -1.85 |
Cell Periphery | 1.74 | 0.07 | 1.16 | -0.16 | 0.86 | 2.79 | 2.56 | -0.82 | -2.42 | -2.30 | 0.39 | -1.06 | 0.29 | -0.07 | 1.12 |
Cytoplasm | 2.09 | 0.81 | 1.64 | -0.74 | 1.42 | 1.82 | 1.10 | -2.11 | -2.37 | -2.29 | 1.53 | -0.72 | 0.62 | -1.15 | 1.47 |
Cytoplasmic Foci | 1.31 | -0.99 | 2.74 | 1.64 | 1.65 | 1.46 | -0.61 | -1.44 | -2.13 | -0.40 | 1.40 | -0.90 | -0.71 | -1.64 | 0.41 |
Eisosomes | 2.03 | 2.32 | 4.78 | 5.68 | 8.10 | 5.26 | 3.67 | 12.42 | 5.22 | 10.33 | 4.07 | 4.90 | 10.07 | 5.99 | 8.88 |
Endoplasmic Reticulum | 3.61 | -0.33 | 5.02 | 1.03 | 7.64 | -1.59 | 0.31 | 2.00 | 2.37 | 1.93 | 2.18 | -1.71 | 4.34 | 1.82 | 8.23 |
Endosome | -0.02 | -0.98 | 1.56 | 0.97 | 1.53 | 1.16 | -0.95 | -2.05 | -2.12 | -0.38 | 0.02 | -1.33 | -1.98 | -1.98 | -1.60 |
Golgi | -0.06 | 0.29 | 1.24 | 1.10 | 0.87 | 1.07 | 0.13 | -1.38 | -1.83 | -1.45 | 0.38 | 0.64 | -1.00 | -1.25 | -1.42 |
Lipid Particles | 0.72 | 1.66 | 1.89 | 1.18 | 1.51 | -0.46 | -0.96 | -1.80 | -1.39 | -0.02 | 0.11 | 1.53 | 0.46 | 0.20 | -1.41 |
Mitochondria | -1.07 | -4.59 | -6.32 | 0.07 | -1.56 | -2.33 | -3.81 | -7.84 | -4.09 | -2.20 | -1.18 | -5.58 | -10.19 | -0.52 | -4.09 |
Mitotic Spindle | -0.84 | -1.76 | -1.97 | -1.95 | -1.32 | 0.90 | -0.98 | -3.36 | -3.38 | -2.44 | -0.29 | -1.85 | -3.89 | -3.88 | -3.22 |
None | 2.31 | 2.82 | 4.58 | 2.26 | 2.93 | -1.72 | 2.04 | -4.64 | -3.27 | -5.26 | 0.36 | 0.79 | 4.07 | 2.16 | 2.79 |
Nuclear Periphery | 1.18 | -3.02 | -3.98 | -4.15 | 0.04 | -1.03 | -1.06 | -3.48 | -2.53 | -0.96 | -0.88 | -3.24 | -5.09 | -4.99 | -0.90 |
Nuclear Periphery Foci | 1.69 | 2.03 | 1.85 | 0.21 | -0.89 | 0.98 | -0.80 | -1.91 | -3.09 | 0.40 | 1.27 | 1.91 | 1.15 | -0.46 | -1.94 |
Nucleolus | 1.64 | 8.12 | 6.06 | 4.13 | -1.63 | -1.63 | 2.19 | 3.26 | 2.24 | 0.21 | 1.40 | 11.13 | 6.94 | 3.79 | -3.73 |
Nucleus | -2.57 | -3.66 | -5.00 | -2.16 | -2.13 | 1.59 | 0.90 | 4.04 | -0.42 | 1.28 | -1.12 | -2.91 | -0.45 | 0.71 | 2.15 |
Peroxisomes | 0.93 | 9.04 | 6.90 | 3.08 | -4.63 | -2.50 | -2.46 | 3.01 | 3.17 | 3.94 | 0.50 | 8.61 | 6.23 | 3.22 | -5.69 |
Vacuole | 1.67 | 1.57 | 2.01 | 1.24 | 1.92 | 0.30 | -0.82 | -2.04 | -2.09 | -0.17 | 1.95 | 1.50 | -0.64 | -2.05 | -1.76 |
Vacuole Periphery | 1.55 | 0.52 | 2.05 | 1.46 | 3.50 | -0.91 | -0.94 | -2.22 | 0.00 | 0.37 | 0.13 | -0.88 | -1.74 | -1.72 | -1.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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