Standard name | |
---|---|
Human Ortholog | |
Description | Amidase; removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 4 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 5 | 5 | 1 | 9 | 5 | 7 | 7 | 7 | 6 | 3 | 22 | 38 |
Bud | 1 | 3 | 12 | 20 | 5 | 12 | 10 | 23 | 19 | 24 | 9 | 15 | 12 | 8 | 12 | 5 | 3 | 0 | 2 | 4 | 3 | 2 | 11 | 17 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 1 | 3 | 9 | 21 |
Bud Site | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 8 | 7 | 5 | 1 | 8 | 1 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 7 | 4 | 1 | 4 | 2 | 2 | 4 | 1 | 3 | 4 | 4 | 4 | 2 | 5 | 6 | 8 | 7 | 2 | 1 | 1 | 0 | 0 | 1 | 2 |
Cytoplasm | 79 | 47 | 32 | 63 | 55 | 36 | 46 | 42 | 26 | 32 | 17 | 27 | 159 | 295 | 340 | 344 | 201 | 121 | 44 | 16 | 7 | 8 | 35 | 31 |
Endoplasmic Reticulum | 0 | 18 | 7 | 3 | 5 | 9 | 7 | 6 | 10 | 6 | 5 | 6 | 20 | 27 | 20 | 21 | 12 | 8 | 15 | 1 | 5 | 1 | 8 | 13 |
Endosome | 110 | 6 | 16 | 48 | 73 | 21 | 38 | 72 | 5 | 5 | 2 | 3 | 47 | 97 | 117 | 48 | 39 | 11 | 91 | 30 | 24 | 33 | 135 | 111 |
Golgi | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 9 | 3 | 6 | 2 | 4 | 2 | 16 | 5 | 4 | 9 | 28 | 21 |
Mitochondria | 87 | 68 | 67 | 159 | 137 | 341 | 385 | 644 | 376 | 442 | 302 | 443 | 63 | 50 | 54 | 27 | 13 | 6 | 276 | 81 | 79 | 97 | 263 | 242 |
Nucleus | 6 | 0 | 0 | 0 | 2 | 3 | 6 | 14 | 5 | 10 | 5 | 8 | 1 | 2 | 2 | 0 | 4 | 4 | 0 | 1 | 0 | 1 | 1 | 4 |
Nuclear Periphery | 2 | 1 | 2 | 1 | 1 | 4 | 8 | 10 | 8 | 10 | 9 | 9 | 0 | 2 | 1 | 1 | 1 | 0 | 6 | 1 | 0 | 0 | 3 | 4 |
Nucleolus | 18 | 2 | 0 | 2 | 1 | 4 | 14 | 39 | 10 | 6 | 3 | 9 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 2 |
Peroxisomes | 15 | 0 | 3 | 8 | 8 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 4 | 5 | 8 | 0 | 0 | 0 | 5 | 1 | 6 | 2 | 11 | 8 |
SpindlePole | 9 | 2 | 7 | 15 | 5 | 6 | 17 | 31 | 8 | 9 | 5 | 6 | 5 | 24 | 12 | 7 | 9 | 9 | 18 | 7 | 6 | 9 | 35 | 24 |
Vac/Vac Membrane | 40 | 24 | 19 | 33 | 51 | 77 | 92 | 131 | 57 | 61 | 29 | 53 | 106 | 131 | 112 | 66 | 62 | 32 | 36 | 16 | 5 | 12 | 35 | 58 |
Unique Cell Count | 300 | 147 | 127 | 278 | 269 | 425 | 499 | 822 | 424 | 492 | 332 | 483 | 363 | 580 | 604 | 465 | 301 | 180 | 546 | 184 | 157 | 196 | 627 | 624 |
Labelled Cell Count | 375 | 178 | 172 | 362 | 349 | 516 | 632 | 1024 | 537 | 616 | 391 | 592 | 434 | 655 | 691 | 539 | 360 | 203 | 546 | 184 | 157 | 196 | 627 | 624 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.2 | 5.1 | 5.2 | 5.6 | 5.1 | 4.5 | 5.0 | 4.7 | 4.4 | 4.7 | 4.3 | 6.1 | 6.0 | 6.1 | 6.6 | 7.2 | 7.8 | 5.6 | 5.6 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.9 | 1.1 | 1.5 | 1.1 | 1.3 | 1.1 | 1.1 | 1.2 | 1.2 | 1.0 | 1.1 | 0.9 | 1.2 | 1.2 | 1.4 | 1.3 | 1.2 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.15 | -0.01 | -0.18 | -0.04 | -0.12 | -0.19 | -0.13 | -0.23 | 0.26 | 0.25 | 0.25 | 0.38 | 0.51 | 0.61 | 0.15 | 0.13 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.8 | -3.5 | -3.2 | 0 | 0 | 0 | 0 | 0 | 0 | -2.8 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -1.1 | -5.0 | -4.9 | -7.9 | -6.1 | -6.2 | -6.2 | -6.7 | 3.7 | 5.3 | 6.4 | 10.1 | 7.9 | 7.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0 | -0.5 | -0.7 | -0.4 |
Endosome | 1.2 | 3.2 | -3.0 | 0 | -1.4 | 0 | 0 | 0 | 0 | 0.1 | 1.1 | 1.8 | -0.7 | 0.1 | -2.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.8 | -0.3 | 6.2 | 0 | 6.2 | 0 | 0 | 0 | 0 | -7.8 | -12.1 | -12.1 | -12.8 | -11.7 | -10.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | -0.7 | 0 | 0 | 0 | -0.2 |
Vacuole | -0.9 | 1.0 | 0.8 | 0.9 | 0.3 | -0.4 | -0.8 | -1.9 | -1.2 | 3.2 | 1.9 | 1.0 | -0.2 | 1.4 | 0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3393 | 2.4551 | 1.8921 | 1.4966 | 1.4423 | 1.9055 | 1.8607 | 2.7943 | 2.3536 | 1.9496 | 2.041 | 2.3692 | 4.5319 | 5.4812 | 4.8895 | 4.9039 | 4.2462 | 4.8762 |
Actin | 0.0119 | 0.0079 | 0.0207 | 0.0211 | 0.0073 | 0.0217 | 0.032 | 0.0159 | 0.0367 | 0.0085 | 0.0347 | 0.018 | 0.0221 | 0.0284 | 0.0413 | 0.0051 | 0.0193 | 0.0091 |
Bud | 0.0014 | 0.004 | 0.0086 | 0.0019 | 0.0019 | 0.0024 | 0.0013 | 0.0045 | 0.0023 | 0.0018 | 0.0072 | 0.0011 | 0.0012 | 0.0041 | 0.0041 | 0.0021 | 0.0042 | 0.0043 |
Bud Neck | 0.0078 | 0.0026 | 0.0036 | 0.0007 | 0.0019 | 0.0117 | 0.0017 | 0.0009 | 0.004 | 0.0012 | 0.0015 | 0.0013 | 0.0011 | 0.0008 | 0.0014 | 0.0004 | 0.0019 | 0.0063 |
Bud Periphery | 0.0028 | 0.0035 | 0.0073 | 0.0029 | 0.0034 | 0.0046 | 0.003 | 0.0073 | 0.0033 | 0.003 | 0.0134 | 0.0014 | 0.0023 | 0.0065 | 0.0047 | 0.0016 | 0.0085 | 0.0028 |
Bud Site | 0.0132 | 0.0331 | 0.027 | 0.0024 | 0.003 | 0.0058 | 0.0052 | 0.0192 | 0.0156 | 0.0045 | 0.0068 | 0.0023 | 0.005 | 0.011 | 0.0078 | 0.0019 | 0.0109 | 0.0017 |
Cell Periphery | 0.0016 | 0.0016 | 0.0016 | 0.0008 | 0.0008 | 0.0016 | 0.0017 | 0.0012 | 0.001 | 0.0008 | 0.0008 | 0.0003 | 0.0014 | 0.0007 | 0.0013 | 0.0002 | 0.0038 | 0.0005 |
Cytoplasm | 0.0169 | 0.0431 | 0.0545 | 0.0278 | 0.0208 | 0.0212 | 0.0216 | 0.0639 | 0.0271 | 0.0246 | 0.0266 | 0.0288 | 0.0152 | 0.0508 | 0.0297 | 0.0058 | 0.0097 | 0.0284 |
Cytoplasmic Foci | 0.0419 | 0.0817 | 0.0785 | 0.0775 | 0.0992 | 0.0647 | 0.0793 | 0.0769 | 0.0661 | 0.0511 | 0.0675 | 0.0579 | 0.0638 | 0.0709 | 0.0492 | 0.0434 | 0.0414 | 0.0532 |
Eisosomes | 0.0011 | 0.0003 | 0.0011 | 0.0002 | 0.0006 | 0.0004 | 0.0018 | 0.0005 | 0.001 | 0.0013 | 0.0007 | 0.0002 | 0.0008 | 0.0003 | 0.0015 | 0.0002 | 0.0014 | 0.0002 |
Endoplasmic Reticulum | 0.0066 | 0.0098 | 0.0099 | 0.0035 | 0.0031 | 0.0081 | 0.0112 | 0.0057 | 0.0039 | 0.0022 | 0.0028 | 0.0016 | 0.0108 | 0.0039 | 0.0039 | 0.0007 | 0.0011 | 0.0049 |
Endosome | 0.1637 | 0.2124 | 0.1563 | 0.1244 | 0.1532 | 0.137 | 0.1422 | 0.1537 | 0.1212 | 0.0832 | 0.1058 | 0.138 | 0.1494 | 0.1234 | 0.0995 | 0.1028 | 0.111 | 0.102 |
Golgi | 0.0943 | 0.1253 | 0.0997 | 0.1012 | 0.1488 | 0.1204 | 0.0978 | 0.1849 | 0.1133 | 0.0429 | 0.1158 | 0.1268 | 0.1266 | 0.1421 | 0.1239 | 0.077 | 0.1573 | 0.1205 |
Lipid Particles | 0.0483 | 0.0119 | 0.0079 | 0.0062 | 0.0032 | 0.0202 | 0.0416 | 0.0101 | 0.0188 | 0.0085 | 0.0251 | 0.0141 | 0.0331 | 0.0017 | 0.0056 | 0.0045 | 0.0035 | 0.0115 |
Mitochondria | 0.4734 | 0.3984 | 0.468 | 0.5753 | 0.5266 | 0.5193 | 0.4491 | 0.4171 | 0.5207 | 0.6909 | 0.4923 | 0.5639 | 0.4826 | 0.4984 | 0.58 | 0.7046 | 0.5916 | 0.6033 |
None | 0.0306 | 0.005 | 0.0054 | 0.0075 | 0.0006 | 0.007 | 0.0149 | 0.0034 | 0.0109 | 0.0459 | 0.0621 | 0.0096 | 0.0121 | 0.0044 | 0.0039 | 0.002 | 0.0124 | 0.0069 |
Nuclear Periphery | 0.0082 | 0.0039 | 0.0035 | 0.0081 | 0.0006 | 0.0097 | 0.011 | 0.0013 | 0.0034 | 0.0006 | 0.0011 | 0.0005 | 0.0087 | 0.0009 | 0.002 | 0.0012 | 0.0002 | 0.0016 |
Nucleolus | 0.0099 | 0.0018 | 0.0013 | 0.0006 | 0.0001 | 0.0017 | 0.0032 | 0.0003 | 0.0006 | 0.0009 | 0.0004 | 0.0002 | 0.0015 | 0.0005 | 0.0003 | 0.0007 | 0.0008 | 0.0006 |
Nucleus | 0.0087 | 0.0032 | 0.0016 | 0.0026 | 0.0004 | 0.0026 | 0.0037 | 0.0009 | 0.0018 | 0.001 | 0.0007 | 0.0005 | 0.0023 | 0.0012 | 0.0017 | 0.0074 | 0.0008 | 0.0011 |
Peroxisomes | 0.0262 | 0.0108 | 0.0232 | 0.0212 | 0.008 | 0.018 | 0.0489 | 0.0222 | 0.0255 | 0.0142 | 0.0245 | 0.0212 | 0.03 | 0.0355 | 0.0291 | 0.0139 | 0.0124 | 0.0314 |
Punctate Nuclear | 0.003 | 0.0012 | 0.002 | 0.0021 | 0.0004 | 0.0012 | 0.0051 | 0.0003 | 0.0028 | 0.0013 | 0.0016 | 0.0002 | 0.0016 | 0.0006 | 0.0005 | 0.019 | 0.0003 | 0.0008 |
Vacuole | 0.0184 | 0.0268 | 0.0102 | 0.0058 | 0.0079 | 0.0107 | 0.0149 | 0.0061 | 0.0133 | 0.0085 | 0.0051 | 0.0076 | 0.0189 | 0.0097 | 0.0053 | 0.0026 | 0.0041 | 0.0052 |
Vacuole Periphery | 0.01 | 0.0118 | 0.0081 | 0.0062 | 0.0083 | 0.0098 | 0.0087 | 0.0038 | 0.0069 | 0.003 | 0.0036 | 0.0045 | 0.0096 | 0.0042 | 0.0033 | 0.0027 | 0.0034 | 0.0037 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.0306 | 17.2293 | 13.3176 | 20.2735 | 10.412 | 9.6314 | 19.961 | 18.1264 | 21.6088 | 16.9498 |
Translational Efficiency | 1.249 | 0.8779 | 0.908 | 0.7627 | 0.8008 | 1.1262 | 0.7338 | 0.862 | 0.7713 | 0.7172 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
243 | 1446 | 2358 | 593 | 107 | 670 | 1960 | 1483 | 350 | 2116 | 4318 | 2076 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 679.04 | 864.25 | 970.23 | 959.08 | 833.69 | 784.48 | 856.26 | 968.75 | 726.32 | 838.99 | 918.50 | 965.99 |
Standard Deviation | 84.88 | 116.97 | 106.13 | 121.72 | 102.82 | 99.00 | 105.71 | 141.76 | 115.37 | 117.60 | 120.18 | 136.40 |
Intensity Change Log 2 | — | 0.347952 | 0.514830 | 0.498155 | — | -0.087774 | 0.038538 | 0.216613 | — | 0.124201 | 0.271919 | 0.349823 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000718 | 0.002168 | 0.003786 | 0.023264 | 0.001981 | 0.002219 | 0.004471 | 0.017031 | 0.001104 | 0.002184 | 0.004097 | 0.018812 |
Bud Neck | 0.015621 | 0.021257 | 0.068396 | 0.015517 | 0.019541 | 0.020505 | 0.022082 | 0.015058 | 0.016820 | 0.021019 | 0.047373 | 0.015189 |
Bud Site | 0.016285 | 0.072953 | 0.077956 | 0.173860* | 0.039677 | 0.050811 | 0.063375 | 0.172047* | 0.023436 | 0.065942 | 0.071338 | 0.172565* |
Cell Periphery | 0.000336 | 0.000219 | 0.000102 | 0.000301 | 0.000610 | 0.000263 | 0.000265 | 0.001119 | 0.000420 | 0.000233 | 0.000176 | 0.000886 |
Cytoplasm | 0.117802* | 0.059775 | 0.241703* | 0.056505 | 0.137991* | 0.033316 | 0.034112 | 0.088454 | 0.123974* | 0.051397 | 0.147475* | 0.079328 |
Cytoplasmic Foci | 0.276367* | 0.198309* | 0.065202 | 0.145354 | 0.275893* | 0.169396 | 0.078624 | 0.136896 | 0.276222* | 0.189154* | 0.071294 | 0.139312 |
Eisosomes | 0.000188 | 0.000111 | 0.000057 | 0.000130 | 0.000230 | 0.000140 | 0.000103 | 0.000125 | 0.000201 | 0.000120 | 0.000078 | 0.000127 |
Endoplasmic Reticulum | 0.003428 | 0.002027 | 0.004560 | 0.002274 | 0.008466 | 0.000921 | 0.001690 | 0.003016 | 0.004968 | 0.001677 | 0.003258 | 0.002804 |
Endosome | 0.029600 | 0.095644 | 0.022385 | 0.072264 | 0.079836 | 0.108280 | 0.124898 | 0.070006 | 0.044958 | 0.099645 | 0.068917 | 0.070651 |
Golgi | 0.047139 | 0.125493* | 0.009891 | 0.093506 | 0.064133 | 0.132000* | 0.078613 | 0.088451 | 0.052334 | 0.127554* | 0.041085 | 0.089895 |
Lipid Particles | 0.027415 | 0.003629 | 0.001760 | 0.001927 | 0.019576 | 0.006125 | 0.005115 | 0.001680 | 0.025018 | 0.004420 | 0.003283 | 0.001751 |
Mitochondria | 0.262798* | 0.209085* | 0.061420 | 0.071821 | 0.120410* | 0.284321* | 0.325728* | 0.112862* | 0.219268* | 0.232908* | 0.181393* | 0.101139* |
Mitotic Spindle | 0.014418 | 0.063722 | 0.147826* | 0.133129* | 0.008720 | 0.039494 | 0.069830 | 0.110115* | 0.012676 | 0.056051 | 0.112423* | 0.116689* |
None | 0.006610 | 0.000917 | 0.001493 | 0.000215 | 0.003537 | 0.000590 | 0.000499 | 0.001247 | 0.005671 | 0.000813 | 0.001042 | 0.000952 |
Nuclear Periphery | 0.000661 | 0.000213 | 0.002820 | 0.000216 | 0.001384 | 0.000158 | 0.000548 | 0.000265 | 0.000882 | 0.000195 | 0.001788 | 0.000251 |
Nuclear Periphery Foci | 0.003934 | 0.000649 | 0.000967 | 0.000495 | 0.002594 | 0.000407 | 0.000643 | 0.000832 | 0.003524 | 0.000573 | 0.000820 | 0.000736 |
Nucleolus | 0.007354 | 0.001860 | 0.003011 | 0.000394 | 0.011384 | 0.000980 | 0.002977 | 0.000592 | 0.008586 | 0.001581 | 0.002996 | 0.000535 |
Nucleus | 0.085872 | 0.009563 | 0.110400 | 0.004997 | 0.061514 | 0.005208 | 0.010995 | 0.007409 | 0.078425 | 0.008184 | 0.065279 | 0.006720 |
Peroxisomes | 0.046724 | 0.053071 | 0.021550 | 0.113971* | 0.039000 | 0.091840 | 0.049786 | 0.067052 | 0.044363 | 0.065347 | 0.034367 | 0.080454 |
Vacuole | 0.022519 | 0.060613 | 0.147463 | 0.079661 | 0.064715 | 0.036424 | 0.104148 | 0.090622 | 0.035419 | 0.052954 | 0.127801 | 0.087491 |
Vacuole Periphery | 0.014212 | 0.018719 | 0.007252 | 0.010199 | 0.038807 | 0.016602 | 0.021498 | 0.015121 | 0.021731 | 0.018049 | 0.013719 | 0.013715 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.12 | -7.64 | -7.44 | -6.92 | -6.52 | -0.37 | -3.53 | -9.01 | -8.85 | -7.25 | -3.90 | -7.90 | -12.27 | -11.40 | -10.09 |
Bud Neck | -2.12 | -15.75 | -0.86 | 1.51 | 17.37 | -0.20 | -0.63 | 0.60 | 1.65 | 3.82 | -1.72 | -11.37 | -0.04 | 2.90 | 18.29 |
Bud Site | -12.08 | -12.09 | -17.70 | -11.16 | -12.53 | -1.46 | -3.50 | -15.25 | -16.69 | -16.85 | -10.48 | -11.65 | -26.46 | -19.36 | -21.17 |
Cell Periphery | 2.85 | 6.22 | 0.84 | -1.24 | -3.78 | 3.71 | 3.88 | -1.14 | -1.96 | -1.96 | 4.57 | 6.31 | -1.46 | -2.07 | -2.27 |
Cytoplasm | 5.03 | -11.28 | 4.61 | -0.47 | 27.39 | 5.57 | 5.48 | 2.02 | -11.86 | -12.83 | 7.38 | -2.97 | 3.45 | -8.51 | 13.31 |
Cytoplasmic Foci | 5.44 | 15.92 | 9.30 | 8.13 | -13.42 | 5.45 | 10.63 | 7.66 | 5.16 | -11.71 | 7.48 | 18.82 | 12.38 | 11.29 | -18.97 |
Eisosomes | 4.66 | 8.64 | 3.57 | -1.54 | -8.90 | 4.21 | 6.14 | 4.88 | 1.60 | -1.93 | 6.10 | 9.94 | 5.55 | -0.31 | -6.63 |
Endoplasmic Reticulum | 1.68 | -1.88 | 0.98 | -1.00 | 4.52 | 3.44 | 3.06 | 2.40 | -3.54 | -2.12 | 3.70 | 1.66 | 2.14 | -2.53 | 1.25 |
Endosome | -14.01 | 3.45 | -7.52 | 5.31 | -12.37 | -2.56 | -4.34 | 1.53 | 6.87 | 12.69 | -11.30 | -4.88 | -4.88 | 9.05 | -0.17 |
Golgi | -11.42 | 7.55 | -4.78 | 5.41 | -13.06 | -6.13 | -1.36 | -1.70 | 7.10 | -0.80 | -12.84 | 2.77 | -5.36 | 9.55 | -12.61 |
Lipid Particles | 5.75 | 6.16 | 6.11 | 2.90 | -0.30 | 4.04 | 4.73 | 6.00 | 2.68 | 3.95 | 6.74 | 7.12 | 7.64 | 4.19 | 3.54 |
Mitochondria | 2.54 | 10.61 | 9.33 | 14.88 | -2.64 | -7.46 | -9.97 | 0.81 | 14.10 | 23.88* | -0.82 | 2.91 | 7.84 | 18.61 | 13.37 |
Mitotic Spindle | -8.99 | -18.38 | -11.80 | -7.04 | -0.25 | -6.75 | -15.72 | -17.86 | -10.78 | -6.58 | -10.69 | -21.31 | -19.04 | -11.39 | -2.64 |
None | 4.96 | 4.39 | 5.59 | 5.90 | 17.08 | 4.18 | 4.40 | 2.62 | -1.22 | -1.43 | 5.85 | 5.54 | 5.21 | -0.35 | 0.37 |
Nuclear Periphery | 3.68 | -12.00 | 2.52 | -1.39 | 14.21 | 1.86 | 1.22 | 1.55 | -3.23 | 2.52 | 3.12 | -5.76 | 2.43 | -3.41 | 14.24 |
Nuclear Periphery Foci | 1.12 | 0.97 | 1.14 | 0.50 | 3.50 | 4.58 | 4.14 | 2.97 | -3.13 | -2.24 | 1.45 | 1.30 | 1.26 | -2.30 | -0.40 |
Nucleolus | 3.80 | 2.81 | 4.81 | 3.95 | 10.37 | 2.48 | 1.98 | 2.55 | 0.94 | 4.08 | 4.33 | 3.33 | 4.96 | 3.75 | 8.58 |
Nucleus | 7.00 | -5.03 | 7.11 | 0.90 | 31.66 | 4.85 | 4.27 | 4.45 | -2.84 | 1.17 | 8.40 | -0.61 | 8.27 | -0.76 | 28.26 |
Peroxisomes | -0.97 | 5.25 | -6.94 | -7.48 | -13.01 | -5.93 | -1.54 | -3.49 | 3.87 | -3.33 | -4.06 | 2.81 | -5.99 | -2.67 | -12.90 |
Vacuole | -8.29 | -27.58 | -10.08 | -5.26 | 8.81 | 2.79 | -4.49 | -4.18 | -12.86 | 0.36 | -4.20 | -22.18 | -12.85 | -11.72 | 8.07 |
Vacuole Periphery | -1.88 | 3.98 | 1.82 | 5.19 | -2.75 | 3.23 | 2.62 | 3.69 | 1.20 | 4.46 | 1.34 | 3.43 | 3.27 | 3.97 | -0.07 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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