Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 4 | 1 | 2 | 11 | 19 | 4 | 6 | 12 | 21 |
Bud | 5 | 2 | 26 | 16 | 9 | 13 | – | 21 | 24 | 27 | 17 | 15 | 1 | 3 | 0 | 3 | 6 | 5 | 1 | 0 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 |
Bud Site | 1 | 1 | 12 | 2 | 6 | 3 | – | 8 | 6 | 6 | 6 | 8 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 17 | 12 | 8 | 3 | 7 | – | 3 | 6 | 8 | 7 | 19 | 14 | 17 | 20 | 21 | 14 | 22 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 4 | 4 | 15 | 12 | 16 | 11 | – | 12 | 10 | 17 | 6 | 6 | 8 | 20 | 16 | 13 | 16 | 14 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 5 | 1 | 5 | 1 | 6 | 7 | – | 5 | 4 | 12 | 11 | 6 | 1 | 10 | 2 | 6 | 4 | 7 | 1 | 1 | 6 | 0 | 0 | 2 |
Endosome | 10 | 24 | 3 | 1 | 1 | 4 | – | 5 | 0 | 1 | 0 | 1 | 14 | 19 | 19 | 8 | 9 | 4 | 22 | 20 | 26 | 12 | 12 | 18 |
Golgi | 36 | 54 | 0 | 0 | 0 | 0 | – | 2 | 1 | 1 | 1 | 1 | 55 | 123 | 139 | 90 | 57 | 70 | 45 | 38 | 15 | 23 | 47 | 63 |
Mitochondria | 567 | 430 | 542 | 428 | 315 | 579 | – | 850 | 810 | 884 | 541 | 622 | 265 | 395 | 318 | 14 | 14 | 22 | 513 | 428 | 461 | 159 | 237 | 294 |
Nucleus | 5 | 5 | 3 | 2 | 7 | 2 | – | 18 | 3 | 8 | 5 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 26 | 14 | 15 | 13 | 22 | 80 | – | 82 | 116 | 89 | 69 | 63 | 14 | 18 | 10 | 3 | 3 | 4 | 4 | 2 | 5 | 1 | 3 | 4 |
Nucleolus | 13 | 14 | 11 | 15 | 5 | 47 | – | 71 | 56 | 55 | 31 | 15 | 4 | 9 | 2 | 4 | 3 | 6 | 0 | 0 | 0 | 0 | 2 | 2 |
Peroxisomes | 2 | 23 | 4 | 3 | 0 | 0 | – | 7 | 2 | 7 | 2 | 4 | 18 | 11 | 16 | 12 | 44 | 16 | 24 | 17 | 26 | 19 | 22 | 35 |
SpindlePole | 3 | 2 | 13 | 3 | 2 | 4 | – | 20 | 4 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 8 | 7 | 0 | 2 | 2 | 1 | 1 | 1 |
Vac/Vac Membrane | 93 | 73 | 31 | 14 | 28 | 23 | – | 36 | 30 | 24 | 19 | 18 | 30 | 39 | 39 | 33 | 26 | 19 | 19 | 16 | 11 | 8 | 11 | 20 |
Unique Cell Count | 623 | 509 | 565 | 445 | 333 | 614 | 925 | 835 | 932 | 569 | 637 | 308 | 490 | 439 | 179 | 170 | 156 | 653 | 554 | 571 | 242 | 358 | 474 | |
Labelled Cell Count | 785 | 668 | 693 | 518 | 420 | 780 | 1141 | 1072 | 1142 | 719 | 785 | 427 | 671 | 588 | 217 | 206 | 199 | 653 | 554 | 571 | 242 | 358 | 474 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 9.1 | 4.8 | 5.7 | 4.0 | 5.5 | – | 5.4 | 5.1 | 5.4 | 5.4 | 5.1 | 9.3 | 8.9 | 8.8 | 10.2 | 10.1 | 9.5 | 9.5 | 9.8 | 9.8 |
Std Deviation (1e-4) | 1.3 | 2.3 | 1.0 | 1.2 | 1.0 | 1.2 | – | 1.3 | 1.4 | 1.4 | 1.3 | 1.2 | 1.6 | 1.5 | 1.6 | 2.4 | 7.1 | 2.3 | 2.0 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.23 | -0.28 | 0.19 | – | 0.15 | 0.08 | 0.17 | 0.15 | 0.07 | 0.95 | 0.88 | 0.87 | 1.08 | 1.06 | 0.99 | 0.97 | 1.02 | 1.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.8 | 0 | -2.4 | – | -2.5 | -1.7 | -1.7 | -1.4 | -2.1 | -3.5 | -4.0 | -4.6 | -1.8 | -0.6 | -0.8 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -2.3 | 0 | -2.5 | – | -2.0 | -2.3 | -2.5 | -1.4 | -1.2 | 0 | -3.2 | -3.1 | 0 | 0 | 0 |
Cell Periphery | -0.4 | 0 | -1.3 | – | -3.4 | -2.3 | -2.1 | -1.2 | 0.9 | 2.0 | 1.3 | 2.2 | 5.4 | 3.8 | 6.2 |
Cytoplasm | 0 | 1.7 | -1.0 | – | -1.9 | -2.0 | -1.1 | -2.0 | -2.3 | -0.1 | 1.3 | 0.9 | 2.8 | 3.8 | 3.6 |
Endoplasmic Reticulum | 0 | 0 | 0.4 | – | 0 | 0 | 0.7 | 1.5 | 0.1 | 0 | 1.6 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.1 | 3.8 | 4.1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 10.4 | 12.7 | 14.4 | 18.0 | 14.3 | 16.8 |
Mitochondria | 0.2 | 0 | -1.3 | – | -3.1 | 1.1 | -1.0 | -0.7 | 1.7 | -5.3 | -7.9 | -10.5 | -23.6 | -23.4 | -21.9 |
Nucleus | 0 | 0 | 0 | – | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.3 | 0 | 6.5 | – | 4.7 | 7.1 | 5.1 | 6.1 | 5.1 | 1.5 | 0.9 | -0.4 | 0 | 0 | 0 |
Nucleolus | 1.4 | 0 | 4.5 | – | 4.7 | 4.1 | 3.6 | 3.1 | 0.5 | -0.7 | -0.1 | -2.1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.6 | 2.1 | 3.3 | 0 | 11.6 | 0 |
SpindlePole | -2.1 | 0 | -2.4 | – | -0.2 | -3.1 | -3.6 | -2.2 | -2.2 | 0 | -2.6 | -2.0 | 0 | 0 | 0 |
Vacuole | -1.8 | 1.7 | -1.4 | – | -1.4 | -1.7 | -2.9 | -1.8 | -2.3 | 2.4 | 1.6 | 2.1 | 5.4 | 4.2 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0416 | 6.6772 | 6.3974 | 6.4402 | 5.7326 | 6.3589 | 5.6215 | 5.2114 | 5.4216 | 5.1257 | 4.4373 | 5.2148 | 8.1962 | 7.4145 | 7.3024 | 7.9423 | 6.7454 | 7.4408 |
Actin | 0.0207 | 0.0115 | 0.007 | 0.0065 | 0.0015 | 0.0024 | 0.0062 | 0.0044 | 0.0015 | 0.0001 | 0.0006 | 0.0007 | 0.0036 | 0.0045 | 0.0026 | 0.0004 | 0.001 | 0.0008 |
Bud | 0.0007 | 0.0008 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0009 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Periphery | 0.0008 | 0.0014 | 0.0009 | 0.0027 | 0.0005 | 0.0002 | 0.0004 | 0.0005 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0007 | 0.0005 | 0.0004 | 0.0001 | 0.0001 |
Bud Site | 0.0039 | 0.0019 | 0.0038 | 0.0003 | 0.0002 | 0.0002 | 0.001 | 0.0007 | 0.0012 | 0 | 0.0001 | 0 | 0.0002 | 0.0012 | 0.0003 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0004 | 0.0005 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0057 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.003 | 0 | 0.0029 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0129 | 0.0054 | 0.0029 | 0.0032 | 0.0016 | 0.0024 | 0.0041 | 0.0049 | 0.0015 | 0.0008 | 0.0075 | 0.0005 | 0.0018 | 0.0048 | 0.0028 | 0.0007 | 0.0004 | 0.0006 |
Eisosomes | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0016 | 0.0009 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0013 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endosome | 0.0261 | 0.0277 | 0.0138 | 0.0159 | 0.0166 | 0.0155 | 0.0123 | 0.015 | 0.0062 | 0.0019 | 0.0226 | 0.0042 | 0.0134 | 0.0211 | 0.0103 | 0.0055 | 0.0007 | 0.0034 |
Golgi | 0.0179 | 0.058 | 0.0211 | 0.0433 | 0.0344 | 0.0172 | 0.0209 | 0.0287 | 0.0098 | 0.0052 | 0.0339 | 0.0141 | 0.0208 | 0.0335 | 0.0221 | 0.0131 | 0.0027 | 0.0144 |
Lipid Particles | 0.0197 | 0.0171 | 0.0147 | 0.003 | 0.0056 | 0.0033 | 0.0103 | 0.0063 | 0.0008 | 0.0004 | 0.0211 | 0.0045 | 0.0244 | 0.0099 | 0.0048 | 0.0009 | 0.0005 | 0.0066 |
Mitochondria | 0.8281 | 0.8409 | 0.9039 | 0.9006 | 0.9213 | 0.9501 | 0.9228 | 0.9251 | 0.9602 | 0.9844 | 0.8651 | 0.97 | 0.9186 | 0.8966 | 0.9397 | 0.9664 | 0.9877 | 0.9651 |
None | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0.0004 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0073 | 0.0049 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0005 | 0.0001 | 0.0007 | 0 | 0.0175 | 0.0001 | 0.0022 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleolus | 0.0014 | 0.0003 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0046 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0083 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.0161 | 0.0105 | 0.0094 | 0.0033 | 0.0025 | 0.0024 | 0.0108 | 0.0106 | 0.0043 | 0.006 | 0.0127 | 0.0033 | 0.0071 | 0.0136 | 0.0063 | 0.0035 | 0.0057 | 0.0058 |
Punctate Nuclear | 0.0059 | 0 | 0.0001 | 0 | 0 | 0.0007 | 0.0015 | 0 | 0.0001 | 0 | 0.0022 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0107 | 0.0058 | 0.0064 | 0.0075 | 0.0031 | 0.001 | 0.0018 | 0.001 | 0.0017 | 0.0002 | 0.0013 | 0.0003 | 0.0015 | 0.0066 | 0.0029 | 0.001 | 0.0001 | 0.0005 |
Vacuole Periphery | 0.0132 | 0.0112 | 0.0106 | 0.012 | 0.0115 | 0.0024 | 0.0053 | 0.0018 | 0.0046 | 0.0006 | 0.0019 | 0.0016 | 0.0047 | 0.0058 | 0.0069 | 0.0074 | 0.0007 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0082 | 11.1834 | 14.9374 | 27.424 | 24.3301 | 22.506 | 27.1381 | 32.8168 | 32.2684 | 31.127 |
Translational Efficiency | 4.0694 | 3.5419 | 2.7414 | 1.6967 | 1.837 | 2.3793 | 1.8189 | 1.8428 | 2.049 | 1.7897 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2215 | 1229 | 143 | 1220 | 1638 | 1553 | 1646 | 1422 | 3853 | 2782 | 1789 | 2642 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 821.34 | 881.60 | 1223.46 | 1160.08 | 703.04 | 910.80 | 1061.63 | 1086.55 | 771.05 | 897.90 | 1074.57 | 1120.50 |
Standard Deviation | 124.19 | 165.84 | 227.35 | 187.62 | 120.50 | 159.05 | 154.81 | 185.73 | 135.87 | 162.73 | 167.65 | 190.17 |
Intensity Change Log 2 | — | 0.102145 | 0.574915 | 0.498173 | — | 0.373528 | 0.594602 | 0.628076 | — | 0.233670 | 0.584028 | 0.559540 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001027 | 0.002764 | 0.005575 | 0.007344 | 0.001256 | 0.003976 | 0.002696 | 0.003581 | 0.001124 | 0.003441 | 0.002926 | 0.005319 |
Bud Neck | 0.000630 | 0.001442 | 0.012206 | 0.001993 | 0.000616 | 0.001400 | 0.005682 | 0.001725 | 0.000624 | 0.001418 | 0.006203 | 0.001848 |
Bud Site | 0.001345 | 0.003778 | 0.011813 | 0.008392 | 0.002106 | 0.003983 | 0.011132 | 0.009846 | 0.001668 | 0.003892 | 0.011186 | 0.009175 |
Cell Periphery | 0.000385 | 0.000317 | 0.000299 | 0.000429 | 0.000495 | 0.000366 | 0.000438 | 0.000505 | 0.000431 | 0.000345 | 0.000427 | 0.000470 |
Cytoplasm | 0.000321 | 0.000639 | 0.000912 | 0.001132 | 0.000270 | 0.000901 | 0.001671 | 0.001010 | 0.000300 | 0.000785 | 0.001610 | 0.001066 |
Cytoplasmic Foci | 0.011129 | 0.025038 | 0.063256 | 0.013721 | 0.005392 | 0.014636 | 0.018395 | 0.013973 | 0.008690 | 0.019231 | 0.021981 | 0.013857 |
Eisosomes | 0.000043 | 0.000083 | 0.000198 | 0.000116 | 0.000036 | 0.000074 | 0.000081 | 0.000093 | 0.000040 | 0.000078 | 0.000090 | 0.000104 |
Endoplasmic Reticulum | 0.000251 | 0.000276 | 0.000745 | 0.001014 | 0.000318 | 0.000198 | 0.000336 | 0.000587 | 0.000279 | 0.000233 | 0.000368 | 0.000784 |
Endosome | 0.029363 | 0.036911 | 0.038397 | 0.017866 | 0.009932 | 0.024637 | 0.032947 | 0.019387 | 0.021102 | 0.030060 | 0.033383 | 0.018684 |
Golgi | 0.022783 | 0.044173 | 0.056250 | 0.068794 | 0.010719 | 0.025485 | 0.022273 | 0.048295 | 0.017654 | 0.033741 | 0.024989 | 0.057761 |
Lipid Particles | 0.004528 | 0.007235 | 0.010136 | 0.002164 | 0.003192 | 0.003815 | 0.005304 | 0.003037 | 0.003960 | 0.005326 | 0.005690 | 0.002634 |
Mitochondria | 0.896915* | 0.834966* | 0.680165* | 0.818642* | 0.943357* | 0.891518* | 0.834729* | 0.856395* | 0.916659* | 0.866535* | 0.822375* | 0.838962* |
Mitotic Spindle | 0.001620 | 0.004939 | 0.020956 | 0.009093 | 0.001293 | 0.001946 | 0.018383 | 0.005385 | 0.001481 | 0.003268 | 0.018589 | 0.007097 |
None | 0.000218 | 0.000274 | 0.001048 | 0.000613 | 0.000452 | 0.000284 | 0.000396 | 0.000426 | 0.000318 | 0.000280 | 0.000448 | 0.000513 |
Nuclear Periphery | 0.000323 | 0.000094 | 0.004433 | 0.000972 | 0.000049 | 0.000065 | 0.000663 | 0.000423 | 0.000206 | 0.000078 | 0.000964 | 0.000676 |
Nuclear Periphery Foci | 0.000246 | 0.000186 | 0.005314 | 0.000938 | 0.000154 | 0.000101 | 0.000684 | 0.000320 | 0.000207 | 0.000138 | 0.001054 | 0.000605 |
Nucleolus | 0.000186 | 0.000150 | 0.001573 | 0.000303 | 0.000644 | 0.000169 | 0.000683 | 0.000212 | 0.000381 | 0.000161 | 0.000754 | 0.000254 |
Nucleus | 0.000276 | 0.000144 | 0.000590 | 0.000139 | 0.001575 | 0.000308 | 0.000443 | 0.000079 | 0.000828 | 0.000236 | 0.000455 | 0.000107 |
Peroxisomes | 0.013169 | 0.028960 | 0.045804 | 0.009896 | 0.013876 | 0.019293 | 0.021706 | 0.011619 | 0.013470 | 0.023564 | 0.023632 | 0.010823 |
Vacuole | 0.005397 | 0.003900 | 0.021581 | 0.015814 | 0.001277 | 0.003500 | 0.012856 | 0.009599 | 0.003645 | 0.003677 | 0.013553 | 0.012469 |
Vacuole Periphery | 0.009847 | 0.003731 | 0.018750 | 0.020625 | 0.002991 | 0.003343 | 0.008505 | 0.013504 | 0.006932 | 0.003514 | 0.009324 | 0.016792 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.94 | -3.70 | -5.13 | -3.66 | -1.05 | -3.08 | -2.60 | -3.57 | 0.38 | -1.09 | -4.35 | -3.55 | -6.26 | -2.31 | -2.96 |
Bud Neck | -2.34 | -4.78 | -4.64 | -1.32 | 4.19 | -2.08 | -6.12 | -2.88 | -0.66 | 4.44 | -3.07 | -7.14 | -4.99 | -1.31 | 5.37 |
Bud Site | -2.93 | -2.60 | -5.09 | -2.97 | 0.80 | -1.76 | -5.69 | -4.13 | -3.04 | 0.57 | -3.35 | -6.76 | -6.36 | -4.18 | 1.14 |
Cell Periphery | 0.59 | 0.74 | -0.35 | -1.11 | -1.28 | 0.96 | 0.40 | -0.08 | -1.34 | -0.60 | 0.99 | 0.03 | -0.41 | -1.72 | -0.46 |
Cytoplasm | -2.12 | -3.46 | -3.95 | -2.15 | -0.97 | -1.93 | -3.80 | -3.16 | -0.29 | 1.64 | -2.49 | -3.88 | -4.86 | -1.20 | 1.58 |
Cytoplasmic Foci | -7.01 | -5.73 | -1.81 | 5.33 | 5.43 | -6.33 | -7.99 | -5.95 | 0.36 | 2.26 | -8.64 | -7.92 | -4.96 | 3.87 | 4.52 |
Eisosomes | -6.27 | -6.01 | -6.14 | -2.66 | 2.92 | -6.11 | -4.94 | -8.77 | -2.40 | -1.14 | -8.36 | -5.91 | -9.67 | -3.58 | -1.29 |
Endoplasmic Reticulum | -0.36 | -2.79 | -2.76 | -2.70 | -0.87 | 1.30 | -0.24 | -1.64 | -2.67 | -1.70 | 0.83 | -1.55 | -3.28 | -3.70 | -2.74 |
Endosome | -2.46 | -1.40 | 4.88 | 6.68 | 3.22 | -6.95 | -9.34 | -5.10 | 2.18 | 5.00 | -4.72 | -5.10 | 1.52 | 6.18 | 6.21 |
Golgi | -6.53 | -3.53 | -9.55 | -4.51 | -1.20 | -6.57 | -5.46 | -10.36 | -5.80 | -6.75 | -8.24 | -3.59 | -13.44 | -7.31 | -9.81 |
Lipid Particles | -1.74 | -2.59 | 2.67 | 3.41 | 3.76 | -0.63 | -1.91 | 0.18 | 0.75 | 1.98 | -1.52 | -1.74 | 2.05 | 3.08 | 3.11 |
Mitochondria | 6.91 | 7.21 | 8.34 | 1.49 | -4.47 | 7.24 | 13.30 | 10.68 | 3.87 | -2.23 | 8.79 | 12.35 | 12.49 | 3.89 | -1.92 |
Mitotic Spindle | -3.03 | -4.61 | -5.49 | -2.54 | 2.66 | -1.52 | -8.72 | -4.86 | -3.95 | 6.22 | -3.27 | -9.34 | -7.19 | -4.31 | 5.82 |
None | -1.24 | -4.11 | -11.28 | -6.52 | 2.13 | 0.82 | 0.30 | 0.15 | -1.89 | -0.88 | 0.40 | -1.48 | -2.28 | -4.90 | -1.84 |
Nuclear Periphery | 2.98 | -3.02 | -2.13 | -2.96 | 2.48 | -1.14 | -5.64 | -3.47 | -3.33 | 1.58 | 2.91 | -4.88 | -3.04 | -4.01 | 1.34 |
Nuclear Periphery Foci | 0.98 | -2.32 | -2.54 | -2.78 | 1.98 | 0.79 | -4.74 | -1.93 | -3.92 | 3.48 | 1.55 | -4.25 | -2.97 | -3.60 | 1.87 |
Nucleolus | 0.31 | -2.32 | -0.62 | -1.07 | 2.13 | 1.44 | -0.09 | 1.30 | -0.37 | 2.29 | 1.39 | -1.56 | 0.77 | -1.02 | 2.59 |
Nucleus | 0.65 | -1.25 | 0.67 | 0.04 | 2.87 | 1.54 | 1.38 | 1.86 | 1.35 | 3.39 | 1.58 | 0.97 | 2.00 | 1.27 | 3.44 |
Peroxisomes | -4.90 | -3.57 | 1.66 | 6.01 | 3.93 | -2.10 | -3.00 | 0.96 | 3.05 | 3.95 | -5.05 | -4.51 | 1.72 | 6.44 | 5.73 |
Vacuole | 2.10 | -4.06 | -5.46 | -6.28 | 1.30 | -4.19 | -10.71 | -7.68 | -5.21 | 2.18 | -0.07 | -9.23 | -8.23 | -8.13 | 0.74 |
Vacuole Periphery | 7.77 | -2.22 | -6.05 | -10.20 | -0.44 | -0.46 | -5.35 | -6.94 | -7.11 | -3.15 | 6.05 | -2.52 | -8.44 | -12.21 | -5.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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