ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.97 0.98 0.95 0.93 0.88 0.86 – 0.74 0.77 0.71 0.68 0.99 0.98 0.99 0.94 0.95 0.95 0.88 0.94 0.93 0.96 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.23 0.06 – 0.28 0.25 0.71 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.1 0.1 0 0.16 – 0.08 0.06 0 0 0 0 0 0.09 0.07 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 9 1 – 1 3 0 0 0 0 0 0 0 0 5 1 1 0 11 9
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 4 0 0 0 1 – 2 5 0 0 0 1 0 2 2 1 0 0 0 0 0 0
Cytoplasm 334 339 226 167 190 158 241 – 192 270 12 15 315 402 415 153 124 116 372 331 219 270 379 341
Endoplasmic Reticulum 3 2 3 0 0 0 1 – 1 1 0 0 1 2 0 6 4 0 2 0 0 0 1 0
Endosome 3 0 1 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 10 2 3 0 2 0
Golgi 2 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 0 5 0 0 0 1 0
Mitochondria 4 2 0 4 3 41 17 – 72 89 12 12 1 0 0 2 1 1 3 3 2 1 0 0
Nucleus 5 1 2 1 2 5 3 – 7 12 1 2 1 1 0 3 4 4 3 3 1 2 5 4
Nuclear Periphery 6 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 3 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 0 2 0 0 1
Vac/Vac Membrane 14 10 4 17 20 5 44 – 20 22 0 0 0 5 8 14 9 8 4 4 0 2 1 2
Unique Cell Count 362 350 231 176 204 180 279 258 351 17 22 317 409 419 163 131 122 421 352 236 282 406 362
Labelled Cell Count 374 355 241 190 215 219 308 296 404 25 29 318 412 423 180 145 131 421 352 236 282 406 362


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.6 7.8 7.4 7.3 4.8 6.2 – 4.9 4.9 4.7 4.9 8.0 8.8 8.2 10.7 10.8 11.0 7.4 8.6 9.2
Std Deviation (1e-4) 1.6 1.6 1.4 1.6 1.9 1.5 2.0 – 1.3 1.1 1.8 1.9 1.7 1.8 1.6 2.3 2.8 2.7 1.8 2.1 2.3
Intensity Change (Log2) – – – -0.07 -0.09 -0.71 -0.34 – -0.66 -0.67 -0.72 -0.65 0.05 0.18 0.08 0.46 0.47 0.5 -0.06 0.15 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -2.4 -4.1 -4.6 – -7.3 -7.0 -5.5 -6.3 1.6 0.4 1.3 -2.0 -1.6 -1.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.8 – 8.7 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 2.0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 3.6 3.7 0 5.4 – 3.1 2.6 0 0 0 0 0 3.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.355 2.2572 1.7533 1.627 1.2083 1.9925 3.0511 4.122 3.7708 3.4087 3.6011 3.6668 5.2886 6.0796 5.8126 5.5439 5.6962 6.2916
Actin 0.0227 0.0003 0.0146 0.0173 0.0048 0.0048 0.0016 0.0008 0.001 0.0064 0.0005 0.0011 0.0116 0.001 0.0266 0.0051 0.0268 0.0006
Bud 0.0009 0.0001 0.002 0.0009 0.0015 0.0003 0.0006 0.0002 0.0005 0.0088 0.0002 0.0002 0.0026 0.0028 0.0006 0.0002 0.0008 0.0009
Bud Neck 0.0013 0.0002 0.0013 0.0012 0.0011 0.0022 0.002 0.0005 0.0009 0.002 0.0007 0.0045 0.0022 0.0003 0.0011 0.0465 0.0007 0.0013
Bud Periphery 0.0012 0.0001 0.0025 0.0012 0.0023 0.0002 0.0004 0.0001 0.0004 0.0115 0.0001 0.0002 0.0021 0.0006 0.0007 0.0002 0.0014 0.0006
Bud Site 0.0028 0.0006 0.0108 0.012 0.0026 0.0001 0.0018 0.0012 0.0008 0.0039 0.0004 0.0002 0.0026 0.0018 0.0028 0.0006 0.0005 0.0003
Cell Periphery 0.0001 0.0001 0.0003 0.0003 0.0001 0 0.0001 0 0 0.0003 0 0 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.6856 0.8164 0.7941 0.7302 0.6221 0.8108 0.7899 0.8544 0.8183 0.7373 0.8838 0.8231 0.7566 0.836 0.7768 0.6681 0.7425 0.8428
Cytoplasmic Foci 0.0249 0.0054 0.02 0.0106 0.043 0.0179 0.0126 0.0069 0.0079 0.0083 0.0085 0.0096 0.0133 0.0054 0.0073 0.0078 0.007 0.0101
Eisosomes 0.0001 0 0.0001 0.0003 0.0002 0 0 0 0 0.0002 0 0 0.0006 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0063 0.0039 0.0073 0.0028 0.0054 0.0027 0.0033 0.0016 0.0035 0.0017 0.0013 0.002 0.0046 0.0016 0.0049 0.0015 0.0044 0.0018
Endosome 0.0162 0.0045 0.0102 0.0082 0.034 0.0054 0.0047 0.0032 0.0043 0.003 0.0023 0.0058 0.0081 0.0009 0.0044 0.0024 0.0229 0.0018
Golgi 0.005 0.0002 0.0013 0.0048 0.0096 0.0026 0.0005 0.0002 0.0002 0.0002 0.0001 0.0002 0.0017 0.0001 0.0022 0.0004 0.0078 0.0002
Lipid Particles 0.0137 0.0002 0.0011 0.0033 0.027 0.0014 0.0007 0 0.0001 0.0003 0 0.0001 0.0011 0 0.0008 0.0001 0.0025 0.0001
Mitochondria 0.0042 0.0002 0.0009 0.0152 0.0112 0.0005 0.0005 0.0003 0.0004 0.0008 0.0002 0.0003 0.0043 0.0003 0.004 0.0003 0.005 0.0062
None 0.1824 0.159 0.1157 0.1608 0.1132 0.118 0.1403 0.0977 0.1214 0.1921 0.0769 0.1191 0.157 0.1221 0.1431 0.2556 0.1426 0.1192
Nuclear Periphery 0.0043 0.0015 0.0035 0.0087 0.0077 0.0044 0.003 0.0015 0.0033 0.0022 0.0014 0.0019 0.0062 0.0023 0.0021 0.0011 0.0044 0.0014
Nucleolus 0.0004 0.0001 0.0003 0.0004 0.0042 0.0002 0.0002 0.0001 0.0002 0.0006 0.0001 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0092 0.0047 0.0072 0.0124 0.0322 0.0176 0.0306 0.0276 0.0306 0.0149 0.0199 0.0272 0.0123 0.0176 0.0098 0.0074 0.0251 0.0079
Peroxisomes 0.0107 0.0001 0.0018 0.0019 0.0558 0.0006 0.002 0.0003 0.0002 0.0003 0.0003 0.0002 0.0065 0.0005 0.0093 0.0005 0.002 0.0002
Punctate Nuclear 0.0047 0.001 0.0024 0.0052 0.0168 0.0093 0.0037 0.0018 0.0043 0.0041 0.0027 0.0031 0.0037 0.0051 0.0013 0.0014 0.0014 0.0037
Vacuole 0.0026 0.0014 0.0019 0.0009 0.004 0.0007 0.0013 0.0013 0.0013 0.001 0.0005 0.0012 0.0019 0.0009 0.0013 0.0006 0.0012 0.0006
Vacuole Periphery 0.0005 0.0001 0.0004 0.0016 0.0011 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1034 12.5767 37.33 42.2526 42.9965 14.0611 28.1576 29.4263 32.6406 31.5217
Translational Efficiency 1.1385 2.2962 1.0551 0.9186 0.7464 1.4564 1.2751 1.0705 1.3331 0.9916

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1330 326 949 151 90 2136 687 1473 1420 2462 1636 1624

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 813.13 804.09 1154.75 1061.00 873.77 903.59 1163.23 1028.88 816.97 890.41 1158.31 1031.87
Standard Deviation 111.91 144.35 163.34 171.53 125.44 126.84 159.12 173.26 113.78 133.62 161.64 173.35
Intensity Change Log 2 — -0.016129 0.506023 0.383867 — 0.048415 0.412811 0.235749 — 0.017663 0.458494 0.309046

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000128 0.000661 0.000344 0.000175 0.000256 0.000552 0.000221 0.000726 0.000136 0.000566 0.000293 0.000675
Bud Neck 0.004681 0.005458 0.001336 0.002573 0.001701 0.006532 0.001418 0.003387 0.004492 0.006390 0.001370 0.003311
Bud Site 0.004160 0.014174 0.002570 0.004592 0.006201 0.012561 0.002832 0.018266 0.004290 0.012774 0.002680 0.016994
Cell Periphery 0.000131 0.000123 0.000108 0.000113 0.000370 0.000095 0.000073 0.000094 0.000146 0.000099 0.000093 0.000096
Cytoplasm 0.628423* 0.654509* 0.493378* 0.532097* 0.581422* 0.694999* 0.521105* 0.652467* 0.625444* 0.689638* 0.505021* 0.641275*
Cytoplasmic Foci 0.166091 0.111391 0.006389 0.007243 0.164787 0.054340 0.006237 0.013145 0.166008 0.061894 0.006325 0.012596
Eisosomes 0.000075 0.000040 0.000015 0.000036 0.000142 0.000030 0.000016 0.000020 0.000079 0.000031 0.000015 0.000022
Endoplasmic Reticulum 0.001466 0.001195 0.002176 0.002748 0.001702 0.000566 0.001374 0.000878 0.001481 0.000649 0.001840 0.001052
Endosome 0.009186 0.010578 0.001034 0.004343 0.022693 0.005454 0.000878 0.005088 0.010042 0.006133 0.000968 0.005019
Golgi 0.001323 0.002084 0.000116 0.000109 0.000572 0.001930 0.000073 0.006115 0.001275 0.001950 0.000098 0.005557
Lipid Particles 0.004214 0.001683 0.000564 0.001249 0.005016 0.001308 0.000590 0.000528 0.004264 0.001357 0.000575 0.000595
Mitochondria 0.003977 0.002215 0.000343 0.000249 0.011652 0.002866 0.000249 0.002034 0.004463 0.002780 0.000303 0.001868
Mitotic Spindle 0.002437 0.004934 0.004454 0.019443 0.000086 0.006713 0.000919 0.049521 0.002288 0.006478 0.002969 0.046724
None 0.004031 0.010007 0.007699 0.013447 0.001913 0.007234 0.010074 0.008727 0.003897 0.007602 0.008696 0.009166
Nuclear Periphery 0.000241 0.000382 0.000792 0.001224 0.000305 0.000282 0.000772 0.000643 0.000245 0.000295 0.000784 0.000697
Nuclear Periphery Foci 0.001171 0.002773 0.002122 0.001562 0.008384 0.000426 0.002492 0.001590 0.001628 0.000737 0.002278 0.001587
Nucleolus 0.000798 0.001411 0.000163 0.000417 0.000725 0.000803 0.000129 0.000465 0.000793 0.000883 0.000149 0.000461
Nucleus 0.103835 0.091145 0.434825* 0.173483* 0.088934 0.148036* 0.380108* 0.147810* 0.102890 0.140503* 0.411848* 0.150197*
Peroxisomes 0.002035 0.004271 0.000078 0.000158 0.001093 0.003325 0.000069 0.000470 0.001975 0.003450 0.000074 0.000441
Vacuole 0.060619 0.079367 0.041379 0.232221* 0.101491 0.051095 0.070303 0.085211 0.063209 0.054839 0.053525 0.098880
Vacuole Periphery 0.000979 0.001597 0.000115 0.002519 0.000553 0.000854 0.000068 0.002815 0.000952 0.000952 0.000095 0.002788

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.56 -10.49 -6.60 0.95 3.63 -1.78 -1.99 -1.05 0.94 -0.12 -3.11 -12.53 -2.80 1.13 0.57
Bud Neck -0.45 5.43 2.29 1.36 -3.15 -6.36 -1.78 -2.87 4.37 -1.95 -2.50 4.98 2.20 4.54 -3.05
Bud Site -4.35 1.90 -1.30 3.41 -2.29 -3.50 2.57 -1.93 1.72 -4.44 -6.27 1.83 -4.23 2.23 -5.18
Cell Periphery 0.78 0.16 2.33 1.01 1.96 7.22 7.11 7.64 1.81 3.32 5.99 3.83 9.72 2.18 5.70
Cytoplasm -0.72 18.19 13.82 12.28 5.25 -4.50 5.70 2.38 20.85 -8.24 -7.13 22.01 13.99 22.95 -6.96
Cytoplasmic Foci 5.76 32.95 32.93 13.44 0.70 7.77 11.30 11.12 21.91 -2.78 20.72 34.57 34.02 25.13 -3.26
Eisosomes 6.73 16.21 3.80 -2.36 -7.88 7.83 8.04 7.96 0.74 -0.88 14.07 17.41 15.13 0.28 -5.49
Endoplasmic Reticulum 0.71 -4.59 -3.94 -4.93 -0.65 3.54 -0.91 1.61 -8.72 5.70 3.39 -4.10 0.26 -9.46 8.28
Endosome -0.61 10.32 8.75 5.25 -2.03 3.14 3.91 3.76 5.73 -2.52 4.36 11.08 9.62 7.19 -2.30
Golgi -1.02 7.50 7.64 3.16 1.61 -3.16 5.06 -1.40 0.52 -2.12 -1.58 7.79 -0.14 0.77 -2.08
Lipid Particles 4.45 6.44 5.26 0.89 -4.73 4.35 4.87 5.03 1.91 1.83 5.58 6.86 6.89 2.15 0.18
Mitochondria 1.16 2.79 2.84 1.61 1.23 0.76 0.99 0.94 3.95 -2.59 1.18 2.93 2.63 4.19 -2.40
Mitotic Spindle -1.34 -1.84 -1.82 -0.98 -1.06 -5.69 -5.42 -9.14 -6.54 -7.94 -2.99 -1.85 -8.13 -6.64 -7.34
None -2.20 -3.19 -2.65 0.06 -1.35 -6.47 -8.55 -10.39 -1.73 -0.13 -3.70 -4.68 -5.82 -1.51 -2.02
Nuclear Periphery -1.64 -21.55 -14.27 -8.81 -5.60 0.55 -16.43 -16.87 -18.49 0.12 -2.29 -28.28 -22.54 -19.77 -2.51
Nuclear Periphery Foci -3.47 -6.73 -4.14 1.17 1.80 5.38 3.63 4.52 -10.46 4.00 5.22 -5.41 -0.68 -7.68 5.45
Nucleolus -1.22 1.73 1.46 3.35 -1.71 -0.84 4.75 1.40 1.89 -1.69 -0.40 1.82 1.00 2.50 -1.78
Nucleus 1.05 -39.73 -6.97 -6.90 11.66 -5.11 -21.54 -10.59 -11.74 17.60 -8.88 -47.95 -20.59 -13.70 26.97
Peroxisomes -1.40 7.78 7.77 2.87 -0.06 -3.65 4.15 3.73 5.62 -2.80 -2.43 8.05 7.66 6.26 -2.71
Vacuole -3.55 -3.37 -12.99 -11.49 -12.22 3.90 -1.99 -5.33 -21.90 -5.42 1.67 -8.54 -22.25 -23.77 -14.66
Vacuole Periphery -0.74 4.06 1.99 1.68 -0.93 -1.95 4.61 -0.65 0.40 -2.52 0.13 4.30 0.85 0.87 -2.50
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YCR090C

YCR090C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycr090c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available