Standard name
Human Ortholog
Description Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.96 0.98 0.92 0.89 0.79 0.75 0.69 0.7 0.58 0.64 0.97 1.0 1.0 0.94 0.9 0.91 0.93 0.94 0.88 0.9 0.94 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.07 0 0.15 0.21 0.19 0.34 0.39 0.42 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.09 0.1 0.13 0.05 0.11 0.06 0 0 0 0.05 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 4
Bud 0 0 5 0 1 1 1 1 1 4 5 3 2 0 0 0 0 0 0 0 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 0 1 1 3 3 9 4 6 0 2 1 3 3 5 0 0 0 0 0 0
Cytoplasm 197 110 181 173 174 225 287 294 232 252 118 186 160 320 335 332 271 270 194 114 162 121 272 239
Endoplasmic Reticulum 0 0 0 0 0 0 2 2 5 2 2 0 1 0 0 15 17 8 1 0 1 0 0 2
Endosome 0 0 3 0 4 1 1 0 0 3 1 1 1 0 0 10 14 9 3 1 5 0 2 8
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 3 0 0 1 0 1
Mitochondria 18 1 6 13 5 38 78 74 115 139 85 138 0 0 0 14 4 1 1 0 4 1 3 3
Nucleus 2 0 0 1 2 2 5 8 6 13 10 11 0 0 1 0 4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 0 0 0 1 2 5 2 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 1 0 1 3 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 4 2 0 0 2 0 2 5
Vac/Vac Membrane 0 1 2 1 14 5 33 40 43 19 23 18 2 0 2 18 28 32 1 0 2 2 1 6
Unique Cell Count 204 111 189 176 189 252 363 392 336 361 202 292 165 320 336 355 301 298 209 121 184 134 291 284
Labelled Cell Count 218 112 198 191 204 273 409 425 406 446 254 365 168 322 339 394 346 330 209 121 184 134 291 284


Ambiguous, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.3 4.9 5.3 7.1 6.7 5.7 6.2 6.0 5.3 5.3 5.3 5.4 5.5 5.5 6.9 7.9 8.2 5.3 5.3 6.4
Std Deviation (1e-4) 0.6 0.9 1.3 1.4 1.6 1.3 1.1 1.5 1.7 1.3 1.4 1.5 1.1 1.1 1.2 1.5 1.7 1.5 1.7 1.1 1.4
Intensity Change (Log2) – – – 0.11 0.55 0.46 0.24 0.36 0.31 0.13 0.14 0.11 0.15 0.18 0.17 0.5 0.7 0.75 0.11 0.12 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 -1.5 -2.5 -5.2 -6.1 -7.2 -7.1 -8.7 -8.1 0.6 3.7 3.3 -1.1 -2.3 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.9 3.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1.8 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 -0.3 4.1 5.7 5.1 8.1 8.9 9.1 10.3 0 0 0 0.5 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.1 0 3.7 4.0 4.6 2.4 4.2 2.7 0 0 0 2.4 3.7 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.4141 -0.2693 -0.8849 -0.8807 -1.0284 -0.7518 1.3502 1.4956 1.4988 1.0796 1.4813 1.5727 2.2672 2.3423 2.2203 2.9202 2.7587 2.2693
Actin 0.0066 0.0063 0.0014 0.0005 0.0304 0.0018 0.027 0.0011 0.0049 0.0002 0.0411 0.0092 0.0266 0.0003 0.0231 0.0238 0.0289 0.0135
Bud 0.0001 0.0001 0 0 0.0002 0 0.0014 0.0025 0.0021 0 0.0007 0.0001 0.0013 0.0086 0.007 0.0004 0.0002 0.0016
Bud Neck 0.0003 0.0001 0.0002 0.0001 0.0012 0.0002 0.0039 0.0001 0.0006 0 0.0013 0.0001 0.0016 0.0001 0.0011 0.0002 0.0002 0.0024
Bud Periphery 0.0001 0.0002 0 0 0.0002 0.0001 0.0031 0.0073 0.0035 0 0.001 0.0002 0.0019 0.0016 0.003 0.0005 0.0003 0.0051
Bud Site 0.0005 0.0009 0.0002 0 0.0013 0.0005 0.009 0.0016 0.0047 0 0.0045 0.0002 0.0104 0.0052 0.0176 0.0016 0.0006 0.0004
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0 0.0003 0.0002 0.0003 0 0.0002 0 0.0004 0.0003 0.0006 0.0001 0.0001 0.0002
Cytoplasm 0.0781 0.2256 0.0867 0.1947 0.1569 0.16 0.0755 0.1558 0.0961 0.1393 0.0755 0.1329 0.0968 0.179 0.1595 0.0876 0.078 0.2302
Cytoplasmic Foci 0.0149 0.0053 0.0082 0.0219 0.0223 0.0046 0.0216 0.0028 0.0136 0.0038 0.0539 0.005 0.011 0.002 0.0068 0.0098 0.0097 0.0173
Eisosomes 0.0027 0.0008 0.0004 0.0001 0.0001 0.0003 0.0006 0.0002 0.0002 0 0.0002 0.0001 0.0005 0.0002 0.001 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.0014 0.0008 0.0011 0.0013 0.0009 0.0006 0.0073 0.0006 0.0012 0.0007 0.0004 0.0004 0.0064 0.0012 0.0021 0.0025 0.001 0.0017
Endosome 0.0031 0.0005 0.0027 0.0036 0.0024 0.0016 0.0198 0.0003 0.004 0.0016 0.0109 0.0027 0.0137 0.0004 0.0022 0.0069 0.0056 0.0053
Golgi 0.0016 0.0002 0.0006 0.0001 0.0018 0.0014 0.0073 0.0001 0.0018 0.0001 0.008 0.0035 0.0054 0.0001 0.0018 0.0036 0.0102 0.0019
Lipid Particles 0.0068 0.0004 0.0051 0.0014 0.0013 0.0019 0.0068 0.0001 0.0035 0.0001 0.0057 0.0057 0.0088 0.0001 0.002 0.0057 0.0209 0.0044
Mitochondria 0.0005 0.0003 0.0006 0.0002 0.0003 0.002 0.0037 0.0012 0.0011 0.0001 0.0023 0.007 0.011 0.0004 0.0041 0.0026 0.0029 0.0043
None 0.8715 0.753 0.8706 0.7669 0.7754 0.8212 0.7866 0.8224 0.8497 0.852 0.7664 0.8294 0.7761 0.7971 0.7553 0.8324 0.8303 0.6898
Nuclear Periphery 0.0005 0.0004 0.0014 0.0004 0.0003 0.0002 0.0052 0.0005 0.0009 0.0003 0.0003 0.0006 0.0075 0.0004 0.0023 0.0029 0.0006 0.0048
Nucleolus 0.0004 0.0001 0.0021 0.0001 0.0001 0.0001 0.0023 0.0002 0.0004 0 0.0003 0 0.0056 0.0004 0.0017 0.0002 0.0002 0.0008
Nucleus 0.0007 0.0013 0.0014 0.0006 0.0003 0.0007 0.0031 0.0008 0.0012 0.0005 0.0004 0.0002 0.0035 0.0015 0.0023 0.0015 0.0003 0.0025
Peroxisomes 0.0074 0.0026 0.0078 0.006 0.0022 0.0015 0.0091 0.0002 0.0063 0.0001 0.0238 0.0016 0.0041 0 0.0014 0.0144 0.0076 0.002
Punctate Nuclear 0.0019 0.0006 0.0086 0.0009 0.0021 0.0009 0.0036 0.0013 0.0023 0.0003 0.0027 0.0006 0.0037 0.0007 0.004 0.0019 0.0018 0.0098
Vacuole 0.0005 0.0004 0.0006 0.0009 0.0002 0.0002 0.0021 0.0006 0.0015 0.0004 0.0005 0.0002 0.0022 0.0004 0.0008 0.0008 0.0002 0.0011
Vacuole Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0008 0.0003 0.0002 0.0001 0.0001 0.0001 0.0016 0.0001 0.0005 0.0003 0.0003 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1308 1.342 7.9666 6.7773 4.3794 9.7526 8.8199 10.9898 10.8756 8.5001
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1841 1769 327 1688 1317 1378 1897 2924 3158 3147 2224 4612

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.78 783.79 976.36 865.78 739.07 728.44 855.78 970.52 715.00 759.55 873.51 932.18
Standard Deviation 79.33 103.33 109.95 112.27 89.45 96.35 105.91 127.83 86.14 104.02 114.75 132.36
Intensity Change Log 2 — 0.167695 0.484641 0.311228 — -0.020901 0.211529 0.393047 — 0.073768 0.350620 0.353892

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000057 0.000260 0.000513 0.001204 0.000041 0.000324 0.000218 0.000716 0.000050 0.000288 0.000262 0.000895
Bud Neck 0.007809 0.022923 0.002526 0.008721 0.001979 0.027840 0.001915 0.022852 0.005377 0.025076 0.002005 0.017680
Bud Site 0.005006 0.013565 0.010338 0.035838 0.001180 0.021172 0.005931 0.039937 0.003410 0.016896 0.006579 0.038437
Cell Periphery 0.000080 0.000090 0.000142 0.000137 0.000122 0.000117 0.000100 0.000174 0.000098 0.000102 0.000106 0.000161
Cytoplasm 0.644591* 0.524846* 0.340231* 0.465501* 0.633510* 0.449644* 0.632928* 0.600349* 0.639970* 0.491917* 0.589892* 0.550994*
Cytoplasmic Foci 0.076199 0.181084* 0.010101 0.015629 0.109287 0.242288* 0.017225 0.003984 0.089998 0.207884* 0.016177 0.008246
Eisosomes 0.000046 0.000081 0.000050 0.000035 0.000065 0.000112 0.000027 0.000015 0.000054 0.000094 0.000030 0.000022
Endoplasmic Reticulum 0.000586 0.001127 0.005817 0.002785 0.000483 0.000504 0.000951 0.000478 0.000543 0.000854 0.001667 0.001322
Endosome 0.002160 0.017695 0.003172 0.008991 0.003474 0.034574 0.001940 0.001441 0.002708 0.025086 0.002121 0.004204
Golgi 0.000601 0.002936 0.000108 0.005288 0.000238 0.008242 0.000075 0.001485 0.000450 0.005260 0.000080 0.002877
Lipid Particles 0.001354 0.004228 0.001916 0.001073 0.002717 0.005122 0.000913 0.000299 0.001922 0.004619 0.001061 0.000582
Mitochondria 0.001515 0.004953 0.000434 0.003940 0.000311 0.014266 0.000368 0.002854 0.001013 0.009031 0.000378 0.003252
Mitotic Spindle 0.002186 0.003706 0.001528 0.090458 0.000044 0.007057 0.007883 0.055632 0.001293 0.005174 0.006948 0.068379
None 0.010386 0.003667 0.004184 0.006347 0.006605 0.002461 0.006696 0.009291 0.008809 0.003139 0.006327 0.008213
Nuclear Periphery 0.000232 0.000431 0.002022 0.002699 0.000315 0.000240 0.000948 0.001244 0.000266 0.000347 0.001106 0.001776
Nuclear Periphery Foci 0.000255 0.000531 0.002947 0.001485 0.000505 0.000377 0.000755 0.000101 0.000359 0.000464 0.001077 0.000608
Nucleolus 0.001115 0.001461 0.000414 0.000608 0.000530 0.001701 0.000370 0.000671 0.000871 0.001566 0.000376 0.000648
Nucleus 0.212831* 0.113366 0.522156* 0.239852* 0.209244* 0.070130 0.256126* 0.229587* 0.211335* 0.094433 0.295241* 0.233344*
Peroxisomes 0.001114 0.007061 0.000304 0.003061 0.000581 0.012950 0.000252 0.000371 0.000892 0.009640 0.000260 0.001356
Vacuole 0.031400 0.094467 0.090898 0.100601 0.028522 0.098117 0.064252 0.028003 0.030199 0.096065 0.068170 0.054574
Vacuole Periphery 0.000476 0.001523 0.000198 0.005748 0.000247 0.002760 0.000127 0.000514 0.000380 0.002065 0.000137 0.002430

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.88 -9.05 -6.45 -4.08 -0.07 -8.99 -2.78 -2.62 -0.25 -1.35 -13.25 -4.54 -4.99 -2.03 -2.30
Bud Neck -8.44 4.66 -1.28 7.96 -7.35 -14.19 -1.45 -17.61 3.17 -17.07 -15.31 5.45 -12.95 5.85 -18.80
Bud Site -5.82 -0.84 -5.94 -1.17 -2.52 -10.27 -3.79 -12.64 0.64 -8.80 -11.29 -1.59 -12.35 -0.89 -9.43
Cell Periphery -1.33 -5.53 -4.56 -2.48 3.53 1.14 4.62 2.47 1.63 -0.87 -0.46 0.28 -0.29 0.03 -0.44
Cytoplasm 14.80 27.36 36.26 18.58 -4.93 18.25 9.20 21.20 -1.07 11.27 23.24 17.99 38.94 10.92 12.60
Cytoplasmic Foci -20.08 22.97 22.87 36.55 -2.50 -19.99 25.97 29.11 42.21 9.38 -28.25 33.19 37.25 55.48 6.66
Eisosomes -7.71 -3.76 -3.61 5.99 2.10 -9.10 10.33 13.51 20.09 5.21 -11.85 6.73 8.62 19.07 1.15
Endoplasmic Reticulum -2.14 -12.25 -12.76 -9.13 7.54 -0.25 -10.51 -10.22 -9.81 4.00 -2.11 -12.24 -12.29 -8.38 3.39
Endosome -12.74 -4.77 -3.78 10.11 -0.69 -15.30 6.10 8.36 16.47 2.45 -19.74 2.94 1.21 19.83 -1.12
Golgi -6.70 8.19 -1.40 2.91 -2.46 -9.04 7.45 -1.26 8.49 -2.06 -10.99 10.68 -1.50 9.20 -3.28
Lipid Particles -7.34 -3.97 -4.33 6.22 2.11 -5.00 8.54 9.32 9.54 0.43 -8.85 6.48 7.00 11.65 -1.10
Mitochondria -4.57 4.62 -0.56 4.17 -4.23 -7.37 -1.01 -14.77 6.25 -14.10 -8.57 4.98 -6.03 7.35 -12.62
Mitotic Spindle -1.22 -0.47 -12.94 -12.56 -12.60 -4.49 -5.02 -18.30 -14.51 -15.02 -3.94 -3.90 -21.93 -19.82 -18.84
None 6.26 7.66 2.14 -5.45 -7.85 4.76 1.33 -9.46 -17.51 -12.09 7.88 4.75 -4.80 -16.31 -11.22
Nuclear Periphery -3.29 -19.45 -17.23 -16.26 -6.35 3.04 -23.59 -29.70 -30.21* -18.37 -2.35 -27.14 -31.83 -30.83 -19.07
Nuclear Periphery Foci -5.54 -9.40 -7.07 -6.33 1.13 1.49 -4.59 -1.56 -2.35 0.87 -2.58 -11.64 -6.99 -6.35 -1.51
Nucleolus -1.65 3.80 -2.98 -2.06 -5.28 -3.68 3.81 -7.62 -4.48 -7.95 -3.81 4.84 -7.69 -5.37 -9.28
Nucleus 19.00 -19.73 -11.70 -26.67 13.81 22.77 -10.92 -16.45 -47.84 -4.58 29.42 -18.30 -21.38 -51.59 1.80
Peroxisomes -10.54 3.30 -1.40 8.43 -3.76 -13.02 4.57 -0.00 12.89 -2.45 -16.61 4.63 -0.51 16.16 -4.58
Vacuole -19.17 -10.59 -29.29 -17.22 -5.47 -18.81 -20.02 -17.80 4.40 7.00 -26.74 -22.64 -32.23 -8.50 -2.34
Vacuole Periphery -7.62 4.32 -1.75 0.44 -2.33 -10.47 6.40 0.71 10.47 -2.48 -12.84 6.92 -1.30 6.78 -2.84
Standard name
Human Ortholog
Description Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gud1

Gud1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gud1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available