Standard name
Human Ortholog
Description Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.92 0.96 0.99 0.99 0.95 0.86 0.92 0.88 0.78 0.74 0.7 0.99 1.0 0.99 0.97 0.96 0.97 0.81 0.89 0.63 0.86 0.84 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.06 0 0 0.12 0.21 0.18 0.32 0.37 0.44 0.47 0 0 0 0.05 0 0 0.09 0.05 0.25 0 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 5 4
Bud 1 0 1 1 0 1 2 4 11 8 4 3 1 1 8 0 1 1 2 0 0 0 8 9
Bud Neck 5 0 2 3 2 7 9 8 2 7 4 3 0 0 0 3 1 6 0 0 0 0 3 2
Bud Site 0 0 0 0 0 0 2 1 0 2 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 2 2 1 4 3 2 1 4 1 2 2 0 0 9 10 22 0 0 1 0 0 2
Cytoplasm 307 91 131 209 267 334 371 433 294 323 139 172 202 455 514 643 371 856 266 107 80 153 274 319
Endoplasmic Reticulum 3 0 1 1 0 0 2 0 0 3 0 1 0 1 2 60 17 63 3 0 1 1 1 1
Endosome 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0 3 6 5 5 0 0 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 4 1 0 0 0 0 0
Mitochondria 9 12 8 6 8 43 88 86 109 153 82 114 2 1 8 34 7 20 28 6 31 7 17 20
Nucleus 0 0 0 0 0 0 0 3 0 0 1 2 0 0 3 2 0 4 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 0 0 0 0 0 1 0 3 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 1 0 0 0 0 0
SpindlePole 3 0 1 0 1 2 6 4 5 3 3 4 0 0 0 2 1 5 4 1 0 0 5 6
Vac/Vac Membrane 0 0 0 0 0 0 2 0 1 1 2 3 0 0 0 11 5 8 5 1 2 0 4 3
Unique Cell Count 320 99 137 211 270 352 429 471 336 415 187 245 204 456 519 665 386 886 330 120 127 178 328 384
Labelled Cell Count 336 104 146 222 279 391 485 542 423 506 237 308 207 458 535 773 421 997 330 120 127 178 328 384


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.2 2.7 2.7 3.0 2.8 2.4 2.6 2.3 2.3 2.6 2.4 3.7 4.0 3.5 5.1 5.2 5.4 4.0 3.8 4.2
Std Deviation (1e-4) 0.6 0.6 0.2 0.9 0.9 1.1 0.5 0.8 0.9 1.0 1.3 1.1 0.9 1.2 1.2 1.8 1.7 1.7 1.1 1.7 1.3
Intensity Change (Log2) – – – 0.05 0.16 0.05 -0.13 -0.02 -0.19 -0.19 -0.06 -0.16 0.49 0.59 0.4 0.93 0.96 1.02 0.58 0.51 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7539 0.1819 0.3593 -0.2287 0.3691 0.0231 1.2896 1.0668 0.8589 0.8523 1.1763 0.5239 -0.5234 -0.6019 -0.791 -0.7266 -0.8822 -0.8684
Actin 0.0433 0.0004 0.0402 0.0164 0.0041 0.0132 0.0455 0.0001 0.0226 0.0006 0.016 0.0032 0.0005 0.002 0.0033 0.0003 0.0419 0.0161
Bud 0.0024 0.0006 0.0022 0.0007 0.0011 0.0025 0.0096 0.0123 0.008 0.0211 0.0053 0.0036 0.0009 0 0.0016 0 0.0018 0.0079
Bud Neck 0.006 0.0001 0.0034 0.0025 0.0017 0.0032 0.0042 0.0001 0.0004 0.0001 0.0011 0.0004 0.0004 0.0001 0.0005 0.0001 0.0108 0.0051
Bud Periphery 0.0069 0.001 0.0071 0.0013 0.0031 0.0048 0.0042 0.0052 0.0059 0.012 0.011 0.0036 0.0018 0.0001 0.0047 0 0.0025 0.006
Bud Site 0.0128 0.0017 0.0104 0.0058 0.0017 0.0018 0.0106 0.0084 0.0082 0.0013 0.011 0.0002 0.0061 0.0004 0.0056 0 0.004 0.0023
Cell Periphery 0.002 0.0006 0.0004 0.0003 0.0006 0.0002 0.0005 0.0002 0.0002 0.0013 0.0001 0.0001 0.0016 0.0001 0.0002 0 0.0007 0.0002
Cytoplasm 0.1227 0.2691 0.1486 0.1654 0.1554 0.2641 0.0886 0.1295 0.1576 0.0572 0.2235 0.2432 0.0233 0.0699 0.0787 0.0377 0.0209 0.1192
Cytoplasmic Foci 0.044 0.0061 0.0229 0.0113 0.0595 0.022 0.0405 0.0046 0.0125 0.0057 0.0334 0.0094 0.0039 0.023 0.0174 0.0015 0.0191 0.0087
Eisosomes 0.001 0.0002 0.0005 0.0004 0.0001 0.0001 0.0007 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0004 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0098 0.0014 0.0023 0.0015 0.0029 0.0017 0.0029 0.0005 0.0018 0.0005 0.0016 0.0006 0.0051 0.0005 0.0009 0.0003 0.0003 0.0007
Endosome 0.029 0.0007 0.0179 0.0192 0.0221 0.0048 0.036 0.0003 0.008 0.0094 0.0476 0.0029 0.0364 0.0058 0.0071 0.0001 0.0067 0.005
Golgi 0.0069 0.0001 0.0155 0.0092 0.0076 0.0022 0.0127 0 0.004 0.005 0.0093 0.001 0.0037 0.0049 0.0061 0 0.0148 0.014
Lipid Particles 0.0137 0.0004 0.0145 0.0065 0.0142 0.0018 0.022 0.0002 0.0025 0.0136 0.0064 0.0029 0.0011 0.0149 0.0012 0.0001 0.0007 0.0027
Mitochondria 0.0119 0.0001 0.011 0.0063 0.0397 0.003 0.0114 0.0003 0.0031 0.006 0.0028 0.0017 0.0226 0.0004 0.0098 0 0.0022 0.0036
None 0.6116 0.7153 0.6718 0.7162 0.6077 0.663 0.6194 0.8359 0.7496 0.8611 0.6135 0.7177 0.8565 0.8566 0.8399 0.9593 0.8708 0.8035
Nuclear Periphery 0.0233 0.0002 0.0046 0.0074 0.0325 0.0007 0.01 0.0001 0.0034 0.0003 0.0014 0.0006 0.0029 0.0001 0.0009 0.0001 0.0001 0.0002
Nucleolus 0.0027 0.0002 0.0007 0.0024 0.0021 0.0007 0.0039 0.0002 0.0007 0.0001 0.0004 0.0002 0.0008 0.0001 0.0016 0 0.0001 0.0002
Nucleus 0.0155 0.0005 0.0011 0.0051 0.0101 0.0014 0.0306 0.0005 0.0026 0.0003 0.0007 0.002 0.004 0.0001 0.002 0.0001 0.0001 0.0006
Peroxisomes 0.0114 0.0001 0.0143 0.0037 0.0064 0.0049 0.0132 0.0001 0.0046 0.0026 0.0075 0.0032 0.0003 0.0198 0.0033 0 0.0013 0.0026
Punctate Nuclear 0.0125 0.0006 0.0079 0.0156 0.0136 0.0018 0.021 0.0007 0.0025 0.0003 0.0052 0.0028 0.0003 0.0008 0.0133 0.0001 0.0003 0.0006
Vacuole 0.0075 0.0007 0.0017 0.0019 0.0093 0.0017 0.0089 0.0006 0.0012 0.001 0.0015 0.0005 0.0225 0.0002 0.001 0.0001 0.0005 0.0004
Vacuole Periphery 0.0031 0 0.0012 0.0009 0.0046 0.0005 0.0037 0.0001 0.0005 0.0004 0.0005 0.0001 0.0049 0 0.0003 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.5387 3.7684 7.2238 7.3536 6.832 15.4482 11.3513 11.0366 9.061 10.1039
Translational Efficiency 0.7127 1.2638 0.3761 0.3492 0.3515 1.1096 0.4979 0.3871 0.5471 0.3001

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1504 1100 205 838 2410 1218 21 140 3914 2318 226 978

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 582.48 652.90 946.34 800.95 684.28 677.95 429.21 813.99 645.16 666.06 898.29 802.82
Standard Deviation 65.04 114.29 112.00 99.24 72.33 83.68 77.23 114.24 85.43 100.17 185.67 101.63
Intensity Change Log 2 — 0.164654 0.700150 0.459504 — -0.013408 -0.672903 0.250424 — 0.071205 0.118865 0.350337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000463 0.000488 0.000860 0.000179 0.000488 0.001525 0.000258 0.000139 0.000476 0.000585 0.000774
Bud Neck 0.015255 0.041547 0.002342 0.011544 0.015352 0.055180 0.012494 0.006699 0.015315 0.048710 0.003285 0.010851
Bud Site 0.003524 0.019498 0.003025 0.033993 0.004934 0.015008 0.040892 0.012945 0.004392 0.017139 0.006544 0.030980
Cell Periphery 0.000142 0.000183 0.000225 0.000175 0.000097 0.000209 0.056591 0.000177 0.000114 0.000197 0.005463 0.000176
Cytoplasm 0.518610* 0.377230* 0.283422* 0.406833* 0.564523* 0.296430* 0.103964* 0.335002* 0.546880* 0.334774* 0.266746* 0.396551*
Cytoplasmic Foci 0.205847* 0.264181* 0.016265 0.028914 0.132068 0.280392* 0.022568 0.033441 0.160418 0.272699* 0.016851 0.029562
Eisosomes 0.000158 0.000163 0.000085 0.000060 0.000088 0.000228 0.001553 0.000072 0.000115 0.000197 0.000222 0.000062
Endoplasmic Reticulum 0.000353 0.000822 0.007156 0.002792 0.000508 0.000825 0.014234 0.002782 0.000448 0.000823 0.007814 0.002791
Endosome 0.009686 0.040511 0.005450 0.008867 0.004182 0.045932 0.000891 0.013501 0.006297 0.043360 0.005026 0.009530
Golgi 0.001220 0.006619 0.000127 0.003413 0.001285 0.009293 0.000540 0.000795 0.001260 0.008024 0.000165 0.003038
Lipid Particles 0.007438 0.010282 0.003346 0.001631 0.004074 0.017303 0.007861 0.002197 0.005367 0.013971 0.003766 0.001712
Mitochondria 0.004340 0.013942 0.000359 0.006638 0.003061 0.023005 0.001170 0.001008 0.003553 0.018704 0.000434 0.005832
Mitotic Spindle 0.000399 0.005754 0.006047 0.056019 0.004444 0.009642 0.000377 0.031904 0.002890 0.007797 0.005520 0.052567
None 0.006101 0.006051 0.003872 0.003755 0.003906 0.002540 0.370177* 0.004662 0.004749 0.004206 0.037909 0.003885
Nuclear Periphery 0.000359 0.000652 0.001891 0.001605 0.000339 0.000591 0.000398 0.001363 0.000347 0.000620 0.001752 0.001570
Nuclear Periphery Foci 0.000258 0.000405 0.003266 0.000991 0.000183 0.000306 0.121213 0.002298 0.000212 0.000353 0.014226 0.001178
Nucleolus 0.002067 0.004414 0.000578 0.000515 0.001391 0.003175 0.008516 0.000500 0.001650 0.003763 0.001315 0.000513
Nucleus 0.169791* 0.104046 0.545704* 0.293320* 0.229966* 0.109808 0.212750* 0.415500* 0.206843* 0.107074 0.514766* 0.310810*
Peroxisomes 0.003956 0.022270 0.000399 0.002205 0.004464 0.023003 0.000867 0.001163 0.004269 0.022655 0.000442 0.002056
Vacuole 0.049588 0.077688 0.115729 0.129792 0.024383 0.102463 0.021277 0.123932 0.034069 0.090706 0.106952 0.128953
Vacuole Periphery 0.000832 0.003279 0.000224 0.006077 0.000574 0.004177 0.000142 0.009801 0.000674 0.003750 0.000217 0.006610

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.16 -8.14 -5.37 -0.39 1.97 -2.80 -3.80 -2.74 0.27 3.02 -4.52 -7.80 -4.82 -0.26 2.93
Bud Neck -8.91 10.45 5.27 11.76 -3.99 -12.00 0.69 6.53 15.55 1.78 -14.80 11.03 6.75 18.57 -2.90
Bud Site -7.63 -1.48 -4.52 1.36 -3.93 -6.28 -3.34 -1.78 2.54 2.96 -9.70 -2.67 -4.43 1.22 -2.23
Cell Periphery -1.10 -3.97 0.52 1.68 5.68 -8.18 -3.76 -2.81 2.69 3.76 -4.75 -3.08 -1.62 3.68 3.07
Cytoplasm 13.05 17.01 18.80 5.43 -3.94 30.68 16.92 12.36 0.55 -5.09 30.76 21.91 26.18 2.97 -4.76
Cytoplasmic Foci -6.93 37.06 37.19 35.62 -1.07 -20.11 11.25 21.86 34.81 0.30 -20.31 42.00 43.96 52.07 -0.95
Eisosomes -0.40 4.89 11.95 9.48 3.06 -11.71 -2.60 -0.51 10.08 2.59 -10.24 -2.13 7.18 15.49 2.73
Endoplasmic Reticulum -3.38 -9.90 -14.75 -11.09 6.22 -2.38 -2.01 -6.77 -6.26 1.38 -3.77 -9.17 -15.56 -13.38 5.86
Endosome -11.56 3.71 6.36 13.65 2.48 -17.56 8.79 -0.76 12.27 -2.24 -20.93 0.55 2.63 21.03 1.69
Golgi -5.93 7.21 0.46 5.51 -1.75 -7.39 2.73 3.42 8.46 1.30 -9.40 6.45 0.85 8.79 -1.71
Lipid Particles -2.20 5.85 11.87 7.52 4.76 -9.50 -0.79 4.98 10.93 1.23 -9.19 1.97 13.33 13.48 4.09
Mitochondria -4.88 5.15 1.67 5.71 -2.04 -10.48 3.38 5.11 12.16 2.12 -11.18 7.38 1.65 10.94 -1.97
Mitotic Spindle -3.86 -1.42 -6.20 -4.81 -3.07 -2.44 4.03 -1.13 -0.22 -1.86 -3.65 -0.85 -5.74 -4.36 -3.14
None 0.05 3.11 2.49 1.98 -1.16 2.60 -4.82 -0.84 -2.20 4.81 0.81 -3.27 1.67 0.43 3.36
Nuclear Periphery -2.67 -14.56 -19.95 -14.15 -2.42 -3.36 -0.72 -9.08 -7.38 -8.12 -4.19 -14.11 -21.70 -17.31 -3.02
Nuclear Periphery Foci -3.77 -6.57 -10.78 -8.40 5.22 -2.51 -3.40 -4.02 -3.78 3.35 -4.48 -3.97 -11.06 -9.49 3.67
Nucleolus -2.69 4.32 4.65 5.20 1.49 -3.34 -2.09 3.99 5.98 2.42 -4.27 1.11 5.95 7.58 2.47
Nucleus 10.18 -17.14 -17.03 -22.76 7.87 19.68 0.26 -5.28 -9.96 -2.26 22.28 -14.15 -15.13 -25.47 6.56
Peroxisomes -10.42 7.96 5.64 12.40 -1.79 -10.25 4.79 5.61 13.31 0.80 -14.40 8.53 6.58 17.85 -1.66
Vacuole -7.39 -8.81 -21.49 -17.68 -4.40 -18.43 0.44 -10.73 -7.37 -10.53 -19.78 -9.61 -25.69 -18.40 -5.86
Vacuole Periphery -6.40 4.98 -1.21 1.75 -1.99 -11.22 4.24 -0.92 0.45 -1.09 -12.49 5.77 -1.68 2.30 -2.26
Standard name
Human Ortholog
Description Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (38%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hbt1

Hbt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hbt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available