Standard name
Human Ortholog
Description Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 0 0 0 0 0 0 0 0.07 0.07 0.11 0.06 0.11 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0.05 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.39 0.42 0.39 0.29 0.54 0.63 0.6 0.77 0.78 0.75 0 0 0 0.12 0.13 0.1 0 0.06 0 0.06 0.12 0.15
Nucleus 0.06 0.07 0 0 0.05 0.06 0.06 0.08 0.07 0.11 0.09 0.09 0.09 0.07 0.1 0.11 0 0 0 0 0 0 0
Nuclear Periphery 0.63 0.6 0.65 0.68 0.64 0.59 0.51 0.4 0.49 0.38 0.49 0.57 0.6 0.51 0.5 0.44 0.49 0.85 0.79 0.87 0.82 0.72 0.62
Nucleolus 0 0 0 0 0 0.06 0.08 0.1 0.12 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.54 0.38 0.22 0.31 0.18 0.17 0.13 0.09 0.1 0.09 0.56 0.54 0.59 0.5 0.5 0.49 0.06 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 2 2 2 0 0 0 0 1 1 0 6 4
Bud 0 0 1 0 1 0 3 5 9 6 7 1 1 3 0 0 0 1 2 3 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1 0 1 0
Bud Site 0 1 0 0 0 2 5 5 7 5 12 0 0 1 0 1 0
Cell Periphery 0 0 0 0 1 0 0 1 0 0 0 4 4 1 0 0 1 0 0 0 0 0 0
Cytoplasm 1 1 16 35 11 22 13 23 12 9 12 77 77 156 8 43 17 0 0 0 0 0 2
Endoplasmic Reticulum 2 4 1 0 3 4 0 0 1 1 1 1 2 7 10 25 10 0 1 3 4 8 5
Endosome 1 0 10 2 27 9 14 22 7 2 7 24 24 25 8 25 5 3 3 12 10 35 61
Golgi 0 0 1 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 2 2 12 25
Mitochondria 24 75 185 139 140 264 495 498 669 319 475 7 7 7 16 52 12 4 12 13 24 82 97
Nucleus 7 14 16 15 22 29 49 68 57 47 56 98 98 92 13 44 5 1 5 3 3 3 2
Nuclear Periphery 79 114 288 241 310 292 400 335 429 156 308 655 691 693 67 178 62 117 155 403 319 492 410
Nucleolus 2 2 6 9 21 30 64 84 105 28 72 3 3 9 6 9 5 0 0 0 1 0 1
Peroxisomes 0 0 0 0 1 0 0 3 2 0 1 0 0 0 0 0 0 0 0 1 3 8 8
SpindlePole 0 1 0 0 0 2 5 8 5 3 2 13 11 28 1 3 0 0 1 2 1 4 5
Vac/Vac Membrane 64 103 166 80 152 87 134 105 74 41 58 636 618 805 67 199 62 7 11 9 11 18 22
Unique Cell Count 126 190 442 356 485 491 787 830 868 411 632 1142 1145 1367 135 401 126 139 198 462 388 682 657
Labelled Cell Count 180 316 690 522 690 741 1182 1157 1377 617 1011 1521 1538 1834 196 579 179 139 198 462 388 682 657


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.8 6.0 6.4 6.0 5.3 4.8 5.1 4.5 4.6 4.6 8.1 8.1 8.2 5.5 5.9 6.8 8.3 8.2 8.4
Std Deviation (1e-4) 0.9 1.0 1.2 2.1 1.4 1.5 1.2 1.3 1.1 1.3 1.2 1.3 1.3 1.5 1.0 1.3 1.3 1.4 1.3 1.3
Intensity Change (Log2) 0.1 0.01 -0.18 -0.33 -0.23 -0.43 -0.37 -0.39 0.44 0.44 0.44 -0.14 -0.04 0.18 0.47 0.45 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.6 -1.2 0.7 -2.2 -0.8 -2.6 -1.2 -1.7 2.4 2.4 4.9 1.2 4.0 4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 5.0 0
Endosome -2.0 2.6 -0.5 0 0.4 0 0 0 -0.2 -0.2 -0.6 0 2.9 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.8 -4.1 3.6 0 6.2 0 0 0 -22.6 -22.6 -24.5 -6.4 -9.3 -6.7
Nucleus 0.4 0.7 1.6 2.0 3.1 2.2 4.4 3.4 3.4 3.4 2.4 2.8 4.1 0
Nuclear Periphery 0.8 -0.4 -1.8 0 -8.4 0 0 0 -2.8 -1.8 -5.3 -3.2 -6.1 -3.2
Nucleolus 1.2 2.7 3.8 0 5.8 0 0 0 0 0 0 0 1.0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 3.0 0 0 0
Vacuole -4.6 -2.0 -6.8 -8.0 -10.3 -12.8 -9.4 -11.3 6.5 5.9 7.8 2.5 3.5 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.4019 4.2192 3.853 3.6133 3.8253 4.0494 2.6214 3.017 2.5646 2.4451 2.3392 2.4939 1.3152 2.2093 2.0624 1.9329 1.5529 1.7294
Actin 0.0092 0.0004 0.0002 0.0003 0.001 0.0014 0.0309 0.0002 0.0005 0.0022 0.0028 0.0001 0.019 0.0018 0.001 0.0046 0.041 0.0003
Bud 0.0001 0.004 0.0001 0.0001 0 0.0002 0.0004 0 0 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0
Bud Neck 0.0023 0.0002 0.0001 0 0.0001 0.0003 0.0005 0 0 0 0.0001 0 0.0007 0.0001 0 0.0001 0.0001 0
Bud Periphery 0.0003 0.0005 0 0 0 0.0003 0.0012 0.0001 0.0001 0.0002 0.0006 0 0.0002 0.0003 0.0002 0.0007 0.0002 0
Bud Site 0.0008 0.0022 0.0002 0.0001 0 0 0.001 0 0 0 0.0001 0 0.0005 0.0003 0.0001 0.0001 0.0002 0
Cell Periphery 0.0002 0.0002 0 0 0 0 0.0002 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0044 0.0131 0.0047 0.0013 0.0006 0.0075 0.0008 0.0003 0.0003 0.0002 0.0001 0 0.0209 0.0002 0.0011 0.0007 0.0001 0.0002
Cytoplasmic Foci 0.0073 0.0018 0.0005 0.0028 0.0003 0.0012 0.0146 0 0.0001 0.0001 0.0016 0.0001 0.048 0.0025 0.0078 0.0002 0.0025 0.0001
Eisosomes 0.0009 0 0 0 0 0 0.0006 0 0 0 0.0002 0 0.0004 0.0004 0.0001 0.0002 0.0002 0
Endoplasmic Reticulum 0.0081 0.0057 0.0039 0.0048 0.0022 0.0031 0.0054 0.0065 0.0065 0.0048 0.0055 0.0013 0.007 0.0135 0.0077 0.0385 0.0059 0.0017
Endosome 0.0206 0.0047 0.0019 0.0018 0.005 0.004 0.0128 0.001 0.0017 0.0017 0.0023 0.0006 0.0106 0.0038 0.0043 0.0038 0.0027 0.0015
Golgi 0.0034 0.0001 0.0001 0.0001 0.0005 0.0005 0.01 0.0002 0.0002 0.0003 0.0041 0.0002 0.0026 0.0012 0.0029 0.0013 0.0027 0.0001
Lipid Particles 0.0059 0.001 0.0004 0.0004 0.0027 0.0009 0.0432 0.0015 0.0016 0.0014 0.1156 0.0149 0.0161 0.0315 0.0153 0.0025 0.0136 0.0008
Mitochondria 0.0022 0.0025 0.0009 0.0014 0.0012 0.0014 0.0204 0.0022 0.0024 0.0035 0.0221 0.0003 0.0044 0.0395 0.0042 0.0229 0.0077 0.0009
None 0.011 0.0083 0.0005 0.0013 0.0007 0.0014 0.0068 0.0002 0.0004 0.0006 0.0009 0.0001 0.0753 0.0005 0.0017 0.0031 0.0007 0.0002
Nuclear Periphery 0.8434 0.7593 0.8321 0.8309 0.8822 0.8547 0.7716 0.8953 0.9092 0.9286 0.7989 0.9598 0.6513 0.8117 0.8844 0.8542 0.8431 0.9671
Nucleolus 0.0031 0.0056 0.0027 0.0027 0.0011 0.0024 0.0053 0.0002 0.0002 0.0002 0.0006 0.0001 0.0047 0.0011 0.0002 0.0005 0.0003 0.0002
Nucleus 0.0301 0.1103 0.0854 0.0296 0.0159 0.0715 0.0081 0.0188 0.0169 0.0047 0.0057 0.0039 0.0421 0.0108 0.0129 0.0107 0.0033 0.0061
Peroxisomes 0.0024 0.0001 0 0.0001 0.0002 0 0.015 0 0 0.0001 0.0013 0 0.0401 0.0017 0.0037 0.0002 0.0379 0
Punctate Nuclear 0.0217 0.0546 0.0427 0.0972 0.0659 0.035 0.0059 0.0003 0.0004 0.0008 0.0011 0.0006 0.0203 0.0021 0.0012 0.0012 0.0018 0.0011
Vacuole 0.0026 0.004 0.0012 0.0007 0.0012 0.0015 0.0019 0.0017 0.0023 0.0007 0.0012 0.0002 0.0028 0.0036 0.001 0.0018 0.0009 0.0003
Vacuole Periphery 0.02 0.0215 0.0225 0.0243 0.019 0.0124 0.0435 0.0714 0.0569 0.0498 0.035 0.0178 0.0329 0.0732 0.0502 0.0526 0.035 0.0194

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.083 14.0301 16.2954 16.5525 18.5794 12.958 16.9613 15.8187 17.4923 18.2481
Translational Efficiency 1.1822 1.3695 1.0419 0.9804 0.9864 1.3309 0.9044 0.7624 0.8268 0.9217

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
84 818 1298 1028 322 572 1831 1408 406 1390 3129 2436

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1053.22 796.50 905.99 926.18 681.84 813.44 941.82 935.96 758.68 803.47 926.96 931.83
Standard Deviation 184.92 144.91 109.29 132.94 103.79 114.19 122.38 130.13 195.58 133.39 118.45 131.41
Intensity Change Log 2 -0.403061 -0.217240 -0.185442 0.254603 0.466018 0.457014 -0.107979 0.090831 0.101976

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000376 0.000269 0.000150 0.000310 0.000273 0.000137 0.000099 0.000104 0.000294 0.000215 0.000120 0.000191
Bud Neck 0.002049 0.000775 0.001151 0.002637 0.000975 0.000187 0.000525 0.000967 0.001198 0.000533 0.000784 0.001671
Bud Site 0.001346 0.000799 0.000225 0.001873 0.000502 0.000234 0.000310 0.000724 0.000677 0.000566 0.000275 0.001209
Cell Periphery 0.000088 0.000091 0.000025 0.000058 0.000093 0.000037 0.000032 0.000032 0.000092 0.000069 0.000029 0.000043
Cytoplasm 0.002851 0.009171 0.001221 0.012679 0.003541 0.002476 0.002930 0.002335 0.003398 0.006416 0.002221 0.006700
Cytoplasmic Foci 0.022656 0.003355 0.000221 0.005564 0.000699 0.001925 0.000662 0.003310 0.005242 0.002766 0.000479 0.004261
Eisosomes 0.000010 0.000009 0.000003 0.000008 0.000009 0.000005 0.000002 0.000004 0.000010 0.000007 0.000002 0.000006
Endoplasmic Reticulum 0.028885 0.016928 0.005831 0.009531 0.021933 0.017465 0.005897 0.006790 0.023372 0.017149 0.005870 0.007947
Endosome 0.044977 0.006562 0.001920 0.010760 0.004656 0.004189 0.001759 0.007863 0.012998 0.005586 0.001826 0.009085
Golgi 0.015415 0.001037 0.000093 0.003550 0.000263 0.001735 0.000215 0.002882 0.003398 0.001324 0.000164 0.003164
Lipid Particles 0.006194 0.010110 0.000702 0.004223 0.023220 0.014459 0.000453 0.003136 0.019697 0.011900 0.000556 0.003595
Mitochondria 0.002606 0.005437 0.001803 0.005880 0.006523 0.006286 0.001759 0.003082 0.005713 0.005787 0.001777 0.004263
Mitotic Spindle 0.081893 0.010076 0.014679 0.134317* 0.004926 0.006222 0.013837 0.072524 0.020850 0.008490 0.014186 0.098601
None 0.000078 0.006720 0.000751 0.001029 0.005651 0.001057 0.000639 0.000620 0.004498 0.004390 0.000686 0.000793
Nuclear Periphery 0.378503* 0.332683* 0.451620* 0.285728* 0.329076* 0.377162* 0.537134* 0.317906* 0.339302* 0.350986* 0.501660* 0.304327*
Nuclear Periphery Foci 0.356116* 0.541393* 0.488730* 0.463276* 0.544665* 0.548193* 0.403132* 0.555335* 0.505655* 0.544191* 0.438641* 0.516486*
Nucleolus 0.002807 0.013462 0.007349 0.005463 0.020433 0.003845 0.003728 0.002286 0.016786 0.009505 0.005230 0.003627
Nucleus 0.016049 0.030774 0.014605 0.025971 0.028987 0.008748 0.019641 0.004631 0.026310 0.021710 0.017552 0.013636
Peroxisomes 0.001027 0.000529 0.000124 0.000664 0.000744 0.000632 0.000066 0.000154 0.000803 0.000571 0.000090 0.000369
Vacuole 0.028355 0.005756 0.004925 0.020501 0.001443 0.002239 0.004807 0.009863 0.007011 0.004309 0.004856 0.014352
Vacuole Periphery 0.007721 0.004063 0.003873 0.005980 0.001387 0.002768 0.002374 0.005452 0.002698 0.003530 0.002996 0.005675

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.64 1.32 -0.09 -1.76 -3.64 1.78 2.25 2.23 1.40 0.01 1.12 2.46 0.93 -0.31 -3.32
Bud Neck 1.42 1.01 -1.11 -4.75 -4.10 1.27 0.57 -0.22 -5.94 -2.76 1.25 0.68 -1.49 -5.46 -4.89
Bud Site 0.75 1.71 -1.40 -2.67 -3.83 0.42 0.45 -0.66 -1.82 -1.77 0.13 1.18 -2.05 -2.81 -4.19
Cell Periphery -0.11 1.65 0.62 1.76 -3.43 2.51 2.59 2.72 0.68 0.21 1.12 3.17 2.37 2.60 -2.22
Cytoplasm -3.60 1.40 -6.97 -3.87 -8.16 0.15 -0.14 0.59 0.42 1.20 -2.62 0.67 -4.99 -2.23 -6.89
Cytoplasmic Foci 1.71 2.02 1.52 -1.51 -5.17 -1.93 -0.03 -3.78 -1.61 -3.40 0.96 2.01 0.38 -1.69 -6.06
Eisosomes 0.28 1.48 -0.79 -2.37 -5.60 2.79 4.92 3.35 0.85 -2.10 1.54 4.56 1.13 -0.62 -5.78
Endoplasmic Reticulum 1.39 2.69 2.18 3.88 -3.67 1.20 4.75 4.57 5.25 -0.72 1.88 5.49 4.77 6.99 -3.04
Endosome 2.39 2.69 2.12 -2.65 -7.65 0.36 2.52 -2.19 -2.79 -5.76 2.05 3.18 1.04 -3.31 -9.27
Golgi 1.41 1.51 1.19 -2.41 -3.72 -1.85 0.42 -2.80 -0.92 -2.85 0.95 1.53 0.15 -2.25 -4.43
Lipid Particles -1.54 2.47 0.66 3.08 -3.81 1.82 5.62 4.89 3.99 -3.84 2.18 5.86 4.82 5.26 -5.41
Mitochondria -1.64 0.46 -1.49 -0.14 -2.80 0.16 4.76 2.94 2.07 -1.74 -0.08 4.55 1.41 1.61 -3.29
Mitotic Spindle 2.94 2.71 -2.43 -15.43 -14.74 -1.01 -4.32 -12.46 -11.17 -10.38 2.05 0.93 -11.54 -18.74 -17.75
None -5.18 -2.05 -5.34 4.15 -1.30 3.25 3.55 3.49 1.04 -0.09 0.14 3.37 3.15 4.38 -1.00
Nuclear Periphery 1.23 -2.12 3.04 5.26 16.84 -2.69 -13.59 0.86 4.74 24.00* -0.77 -11.57 3.06 6.29 29.02
Nuclear Periphery Foci -5.88 -4.30 -2.48 7.79 4.48 -0.07 8.90 -0.36 -0.35 -15.65 -2.37 4.66 0.27 4.37 -8.39
Nucleolus -3.76 -2.08 -2.02 2.69 0.07 3.98 4.07 4.43 1.08 1.87 2.11 3.47 3.74 3.56 1.12
Nucleus -1.29 0.08 -2.04 -1.53 -5.49 3.62 1.10 4.44 1.48 6.51 0.84 1.36 1.28 0.63 -0.14
Peroxisomes 0.73 1.37 0.21 -1.00 -2.38 0.48 4.17 3.49 2.80 -1.46 1.06 3.80 1.48 0.71 -2.68
Vacuole 2.59 2.68 0.33 -6.89 -7.35 -1.41 -3.34 -6.18 -4.62 -3.39 1.15 0.99 -4.03 -7.81 -7.90
Vacuole Periphery 1.26 1.35 0.77 -1.29 -1.63 -1.98 -1.95 -5.05 -2.60 -3.37 -0.97 -0.37 -3.32 -2.46 -3.66
Standard name
Human Ortholog
Description Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination
Localization
Cell Percentages nuclear periphery (73%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gle1

Gle1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gle1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available