Standard name
Human Ortholog
Description Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Sec6p, Yip3p, and Sbh1p; less abundant than RTN1; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.07 0.11 0.08 0.11 0.05 0.07 0.07 0.11 0.12 0.09 0.15 0 0.17 0.13 0.08 0.08
Bud 0 0 0 0 0 0.05 0.08 0.11 0.09 0.1 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0 0.05 0.06 0 0.05 0.12 0.07 0.08 0.1 0.15 0.19 0.12 0.14 0.16 0.05 0 0.09 0.07 0.06 0.06
Cytoplasm 0.84 0.96 0.92 0.92 0.8 0.54 0.41 0.38 0.27 0.23 0.2 0.21 0.88 0.76 0.71 0.79 0.77 0.73 0.34 0.74 0.3 0.46 0.61 0.59
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.05 0.13 0.1 0.08 0.12 0 0.06 0.1 0 0.1 0.09 0.05 0 0 0.06 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0.06 0.1 0.19 0.55 0.57 0.54 0.62 0.64 0.74 0.66 0 0 0 0.05 0.05 0 0.23 0.13 0.29 0.12 0.1 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 3 0 5 3 19 12 33 59 36 46 20 27 23 14 18 9 19 8 84 33 25 24
Bud 2 2 8 2 14 23 38 44 45 55 46 45 3 1 3 4 5 1 2 3 6 8 2 8
Bud Neck 1 2 9 1 4 1 1 2 0 0 0 0 0 2 2 0 4 2 9 3 13 12 10 9
Bud Site 0 0 0 1 0 3 1 3 9 5 6 6 1 0 0 0 0 0
Cell Periphery 5 2 19 16 9 23 28 11 27 62 32 36 38 59 64 16 20 16 6 3 43 17 17 17
Cytoplasm 99 234 423 311 258 236 206 148 134 122 90 94 332 303 243 102 113 75 44 179 151 116 183 179
Endoplasmic Reticulum 3 2 2 0 10 18 27 19 64 50 34 53 6 25 35 5 14 9 6 1 17 14 11 9
Endosome 0 0 1 0 4 7 4 16 6 2 3 2 0 2 1 3 2 2 2 3 12 4 1 3
Golgi 0 1 0 0 0 0 1 2 8 12 7 14 1 5 4 2 1 0 0 1 3 0 2 1
Mitochondria 23 6 26 34 60 240 287 212 313 338 333 290 1 1 5 7 8 3 29 31 142 29 30 35
Nucleus 0 0 1 2 0 0 2 4 2 5 2 6 2 4 1 1 6 1 0 0 0 2 0 0
Nuclear Periphery 0 1 0 0 1 3 3 3 8 10 9 11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 5 2 2 4 4 3 0 0 0 0 0 1 0 0 0 0 1 1
Peroxisomes 0 0 3 6 6 4 1 9 4 5 1 2 3 4 4 1 0 2 0 0 3 0 0 0
SpindlePole 1 1 5 7 2 7 9 9 9 10 9 6 4 13 5 0 1 2 1 0 3 2 1 0
Vac/Vac Membrane 0 0 1 1 3 12 23 16 26 27 19 17 2 8 5 0 0 0 4 2 4 7 8 6
Unique Cell Count 118 243 458 339 322 439 504 393 503 525 448 438 378 401 342 129 146 103 131 244 500 257 301 305
Labelled Cell Count 138 252 501 381 376 581 655 512 690 766 631 631 413 454 395 155 192 123 131 244 500 257 301 305


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 3.7 3.8 4.1 3.9 4.6 4.9 5.1 6.5 6.0 8.3 6.3 7.2 7.3 6.3 6.0 6.1 5.7 6.0 5.8
Std Deviation (1e-4) 0.6 1.4 1.2 2.3 1.9 3.6 3.3 2.7 3.0 5.7 5.6 8.9 2.8 4.3 3.6 2.1 1.7 2.9 1.9 3.7 2.0
Intensity Change (Log2) 0.01 0.13 0.08 0.32 0.39 0.46 0.81 0.68 1.16 0.76 0.95 0.97 0.76 0.7 0.71 0.61 0.7 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1269 1.357 1.0062 0.2554 0.307 1.0873 1.2092 1.657 1.0803 1.3542 0.6994 1.5093 1.3931 1.923 0.9135 1.7516 0.8419 0.9557
Actin 0.0691 0.0437 0.06 0.0937 0.1035 0.0359 0.1367 0.052 0.0821 0.0916 0.1264 0.0879 0.0824 0.0672 0.0881 0.044 0.083 0.0655
Bud 0.0025 0.0036 0.0114 0.0024 0.0064 0.0029 0.0068 0.0071 0.0098 0.0022 0.0051 0.0043 0.0085 0.0172 0.0227 0.0149 0.0099 0.0096
Bud Neck 0.009 0.0044 0.013 0.009 0.0116 0.0586 0.0297 0.0046 0.0107 0.0512 0.0678 0.1002 0.0084 0.0083 0.0117 0.0458 0.0329 0.0663
Bud Periphery 0.0198 0.0129 0.0667 0.0187 0.0274 0.029 0.0268 0.023 0.0427 0.009 0.0461 0.0307 0.0546 0.0357 0.0948 0.149 0.0745 0.0738
Bud Site 0.038 0.0698 0.0422 0.01 0.0035 0.0031 0.0464 0.1646 0.0569 0.0085 0.0057 0.0108 0.0658 0.2009 0.0726 0.0205 0.0071 0.0094
Cell Periphery 0.0795 0.0489 0.0555 0.0048 0.0223 0.0566 0.0313 0.0729 0.0755 0.055 0.0521 0.0913 0.1478 0.0695 0.0931 0.2076 0.0934 0.1095
Cytoplasm 0.2083 0.2739 0.2256 0.1592 0.2264 0.332 0.1832 0.3166 0.2898 0.2547 0.4102 0.3155 0.2495 0.2955 0.2472 0.1496 0.2859 0.2999
Cytoplasmic Foci 0.0209 0.0093 0.0186 0.0176 0.0081 0.0212 0.0663 0.0224 0.0255 0.0201 0.0505 0.0195 0.0258 0.0116 0.0161 0.0061 0.0207 0.0201
Eisosomes 0.0091 0.0047 0.0058 0.0029 0.0092 0.0069 0.0027 0.0033 0.0026 0.0048 0.0062 0.003 0.0086 0.0154 0.0039 0.0048 0.011 0.0058
Endoplasmic Reticulum 0.0182 0.0063 0.0075 0.001 0.0051 0.0088 0.0218 0.0089 0.0166 0.0122 0.0063 0.0091 0.0266 0.0102 0.0183 0.0198 0.0139 0.0113
Endosome 0.0128 0.0083 0.0076 0.0024 0.0047 0.0037 0.0346 0.0037 0.0092 0.0236 0.0096 0.0127 0.0258 0.0035 0.0112 0.016 0.0258 0.0089
Golgi 0.0085 0.0027 0.0047 0.0013 0.0114 0.0051 0.0208 0.0057 0.0083 0.011 0.026 0.0133 0.0087 0.0054 0.0099 0.017 0.019 0.0058
Lipid Particles 0.0094 0.0051 0.0037 0.002 0.0042 0.0032 0.0207 0.0077 0.0043 0.0282 0.0085 0.0086 0.0082 0.0074 0.0074 0.0066 0.0142 0.0056
Mitochondria 0.0029 0.0007 0.0034 0.0015 0.003 0.0035 0.0075 0.0028 0.0057 0.0116 0.0136 0.0166 0.0048 0.0025 0.0099 0.0389 0.0137 0.0044
None 0.4627 0.4995 0.463 0.6343 0.5465 0.4242 0.3049 0.2974 0.3402 0.3911 0.1334 0.2655 0.2419 0.2417 0.2752 0.2445 0.24 0.2796
Nuclear Periphery 0.0043 0.0005 0.0007 0.001 0.0014 0.0003 0.0074 0.0006 0.0015 0.0072 0.0005 0.0013 0.0089 0.0008 0.0021 0.0012 0.0116 0.0062
Nucleolus 0.0007 0.0004 0.0004 0.0025 0.0002 0.0004 0.0012 0.0008 0.0007 0.0003 0.0002 0.0005 0.0013 0.0005 0.0005 0.0008 0.0005 0.0007
Nucleus 0.0058 0.001 0.0011 0.0031 0.0006 0.0007 0.0028 0.0008 0.0013 0.0013 0.0004 0.0018 0.0051 0.0019 0.0019 0.0017 0.0025 0.0023
Peroxisomes 0.0094 0.0003 0.005 0.0034 0.0024 0.001 0.028 0.0011 0.0092 0.0063 0.0252 0.0016 0.0036 0.0011 0.0051 0.0021 0.0264 0.0068
Punctate Nuclear 0.0058 0.0004 0.0007 0.0285 0.001 0.0006 0.0133 0.0006 0.0029 0.0067 0.0039 0.0024 0.0022 0.0007 0.0012 0.0009 0.0023 0.0018
Vacuole 0.0028 0.0037 0.0028 0.0007 0.001 0.0021 0.0059 0.0032 0.0041 0.0024 0.0018 0.0027 0.0095 0.0028 0.0057 0.007 0.0073 0.0054
Vacuole Periphery 0.0005 0.0002 0.0003 0.0001 0.0003 0.0004 0.0012 0.0002 0.0004 0.0011 0.0004 0.0005 0.0016 0.0002 0.0014 0.0015 0.0043 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5345 1.669 5.5731 5.1578 1.8155 6.5309 6.961 4.8406 7.7289 6.4519
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1491 85 2308 753 1470 1612 219 1241 2961 1697 2527 1994

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 656.60 809.19 844.38 707.78 651.42 691.91 873.74 812.85 654.03 697.78 846.92 773.17
Standard Deviation 64.59 109.01 153.41 165.84 80.75 122.71 106.31 142.01 73.11 124.72 150.14 159.79
Intensity Change Log 2 0.301464 0.362878 0.108286 0.086996 0.423616 0.319401 0.198635 0.393446 0.217285

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000790 0.002491 0.014987 0.009832 0.000880 0.001881 0.008872 0.041909 0.000835 0.001912 0.014457 0.029796
Bud Neck 0.021720 0.049124 0.018202 0.011484 0.016856 0.037765 0.005976 0.019402 0.019305 0.038334 0.017143 0.016412
Bud Site 0.005955 0.029714 0.064070 0.044020 0.011924 0.015795 0.030713 0.105655* 0.008918 0.016493 0.061180 0.082380
Cell Periphery 0.000340 0.020153 0.021725 0.005419 0.002315 0.000764 0.004353 0.043712 0.001321 0.001735 0.020220 0.029251
Cytoplasm 0.443798* 0.241902* 0.345961* 0.414259* 0.516508* 0.403935* 0.254276* 0.383930* 0.479895* 0.395819* 0.338015* 0.395383*
Cytoplasmic Foci 0.265728* 0.365473* 0.013273 0.082633 0.232371* 0.289163* 0.043831 0.044495 0.249168* 0.292985* 0.015921 0.058898
Eisosomes 0.000614 0.003753 0.001898 0.000815 0.000732 0.000757 0.000554 0.011016 0.000673 0.000907 0.001781 0.007164
Endoplasmic Reticulum 0.001099 0.001102 0.010998 0.006100 0.002653 0.000931 0.009532 0.015292 0.001870 0.000940 0.010871 0.011820
Endosome 0.010843 0.039069 0.001456 0.007611 0.008833 0.023213 0.004372 0.003952 0.009845 0.024007 0.001708 0.005334
Golgi 0.004962 0.012182 0.000341 0.003488 0.004405 0.007417 0.000330 0.002001 0.004686 0.007656 0.000340 0.002563
Lipid Particles 0.012913 0.006112 0.002268 0.009555 0.008913 0.009241 0.004645 0.003510 0.010927 0.009084 0.002474 0.005793
Mitochondria 0.006839 0.003782 0.001072 0.000968 0.005161 0.011550 0.000303 0.003630 0.006006 0.011161 0.001005 0.002625
Mitotic Spindle 0.002284 0.002240 0.002212 0.005352 0.000848 0.005750 0.000121 0.005085 0.001571 0.005574 0.002030 0.005186
None 0.001904 0.000309 0.002415 0.013225 0.003181 0.002504 0.006188 0.001871 0.002538 0.002394 0.002742 0.006159
Nuclear Periphery 0.000545 0.000174 0.001250 0.000775 0.000276 0.000321 0.001353 0.000498 0.000411 0.000314 0.001259 0.000603
Nuclear Periphery Foci 0.000542 0.000685 0.000645 0.001351 0.000395 0.000271 0.002115 0.001628 0.000469 0.000292 0.000773 0.001523
Nucleolus 0.001039 0.000758 0.000231 0.000850 0.000629 0.000842 0.000745 0.000192 0.000835 0.000838 0.000275 0.000440
Nucleus 0.161487* 0.062032 0.448508* 0.250033* 0.140865* 0.102707 0.482011* 0.174400* 0.151249* 0.100670 0.451411* 0.202962*
Peroxisomes 0.011894 0.043472 0.000801 0.003601 0.005292 0.017288 0.005247 0.003122 0.008616 0.018599 0.001186 0.003303
Vacuole 0.043954 0.114236 0.047544 0.128186 0.035791 0.066174 0.134290 0.132793 0.039901 0.068581 0.055062 0.131053
Vacuole Periphery 0.000749 0.001236 0.000143 0.000442 0.001175 0.001731 0.000175 0.001908 0.000961 0.001706 0.000146 0.001354

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.22 -7.68 -3.50 -2.54 1.88 -2.01 -1.94 -10.48 -10.05 -6.90 -2.39 -7.88 -10.93 -10.19 -4.28
Bud Neck -2.56 2.43 5.80 3.72 3.78 -7.80 6.82 0.40 8.49 -7.19 -7.74 2.28 3.85 10.38 1.18
Bud Site -3.34 -13.56 -6.66 -1.05 3.05 -1.85 -1.37 -13.13 -12.54 -7.22 -4.64 -12.80 -14.93 -12.52 -2.57
Cell Periphery -1.51 -8.89 -2.82 1.20 5.75 1.77 -0.18 -8.11 -8.79 -7.50 -0.47 -8.20 -8.66 -8.30 -1.57
Cytoplasm 7.62 13.27 4.96 -5.09 -4.80 12.20 18.39 15.08 3.95 -8.59 10.54 22.61 14.04 2.78 -6.33
Cytoplasmic Foci -3.47 45.17 27.57 10.61 -14.79 -7.41 28.70 33.86 41.34 -0.75 -6.49 60.08 44.77 40.73 -17.22
Eisosomes -1.34 -4.08 -0.30 1.32 3.42 -0.09 1.74 -7.40 -7.34 -7.75 -1.03 -3.67 -7.38 -6.73 -5.80
Endoplasmic Reticulum -0.16 -9.98 -3.09 -3.03 2.83 3.07 -3.22 -6.57 -8.11 -1.20 2.83 -9.41 -7.51 -8.52 0.19
Endosome -3.50 12.36 3.81 4.04 -6.48 -9.03 5.99 7.63 12.69 2.12 -9.11 16.92 8.36 12.63 -6.61
Golgi -2.15 7.41 1.59 2.72 -1.81 -3.63 9.18 5.38 7.91 -3.68 -3.79 11.31 4.24 6.71 -3.04
Lipid Particles 5.28 10.68 2.88 -1.33 -4.31 -0.32 5.46 9.47 8.44 3.85 2.18 13.98 7.55 4.74 -4.67
Mitochondria 2.33 5.51 5.66 2.94 0.68 -4.54 5.84 2.20 6.53 -3.24 -4.05 7.34 4.89 7.80 -2.09
Mitotic Spindle 0.20 0.09 -1.62 -1.56 -1.78 -3.36 1.54 -2.40 0.90 -3.08 -2.83 -0.85 -2.66 0.66 -2.19
None 5.88 -1.07 -4.08 -4.85 -3.87 0.76 -0.70 2.44 1.05 1.69 0.20 0.13 -2.90 -2.54 -2.97
Nuclear Periphery 3.09 -8.12 -3.92 -10.66 5.14 -0.99 -13.21 -8.78 -6.49 8.31 1.38 -15.90 -6.51 -9.45 11.18
Nuclear Periphery Foci -0.51 -0.85 -3.03 -2.09 -4.01 2.09 -6.74 -9.49 -10.60 2.04 1.34 -2.97 -7.13 -12.34 -5.85
Nucleolus 0.88 4.95 1.72 0.12 -6.17 -2.04 -0.01 5.95 8.24 4.03 0.00 6.20 4.66 5.46 -3.26
Nucleus 7.55 -38.97 -9.86 -12.88 18.86 7.38 -15.98 -8.80 -14.52 12.56 11.64 -45.53 -12.59 -20.44 29.74
Peroxisomes -3.18 9.80 6.95 4.14 -4.29 -9.95 0.88 5.47 12.90 0.32 -7.52 11.71 8.82 13.25 -4.83
Vacuole -4.47 -6.06 -15.78 -3.11 -13.20 -10.65 -10.63 -23.34 -17.92 0.89 -10.67 -11.46 -27.90 -20.51 -18.86
Vacuole Periphery -2.03 9.24 4.05 3.72 -3.60 -2.42 5.27 -0.33 0.57 -2.05 -4.73 8.14 -0.11 1.80 -2.34
Standard name
Human Ortholog
Description Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Sec6p, Yip3p, and Sbh1p; less abundant than RTN1; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rtn2

Rtn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available