Standard name | |
---|---|
Human Ortholog | |
Description | Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph21p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 1 | 1 | 2 | 4 | 12 |
Bud | 2 | 0 | 1 | 2 | 0 | 2 | 9 | 10 | 8 | 7 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 2 | 2 | 9 | 21 |
Bud Neck | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 5 | 8 | 3 | 6 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 673 | 171 | 305 | 363 | 510 | 462 | 487 | 483 | 379 | 430 | 222 | 294 | 233 | 286 | 315 | 91 | 113 | 136 | 656 | 155 | 298 | 181 | 235 | 185 |
Endoplasmic Reticulum | 1 | 2 | 3 | 2 | 1 | 0 | 0 | 1 | 2 | 3 | 0 | 4 | 2 | 0 | 0 | 4 | 5 | 3 | 2 | 1 | 3 | 3 | 4 | 2 |
Endosome | 5 | 1 | 2 | 10 | 6 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 5 | 2 | 2 | 25 | 29 | 26 | 8 | 1 | 4 | 6 | 28 | 59 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 4 | 0 | 1 | 1 | 6 | 11 |
Mitochondria | 13 | 0 | 2 | 11 | 29 | 90 | 148 | 112 | 207 | 128 | 161 | 73 | 0 | 1 | 0 | 3 | 5 | 5 | 3 | 2 | 13 | 5 | 10 | 19 |
Nucleus | 4 | 3 | 8 | 8 | 6 | 18 | 20 | 25 | 15 | 23 | 14 | 24 | 2 | 0 | 1 | 10 | 5 | 4 | 20 | 11 | 8 | 9 | 16 | 15 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 8 | 8 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 5 |
SpindlePole | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 9 | 16 | 8 | 13 | 1 | 4 | 4 | 29 | 56 |
Vac/Vac Membrane | 89 | 15 | 27 | 76 | 78 | 80 | 96 | 101 | 85 | 89 | 29 | 58 | 34 | 50 | 31 | 64 | 88 | 75 | 9 | 4 | 15 | 6 | 12 | 11 |
Unique Cell Count | 733 | 188 | 332 | 423 | 583 | 581 | 668 | 646 | 584 | 617 | 345 | 423 | 259 | 303 | 329 | 173 | 207 | 218 | 744 | 190 | 360 | 230 | 373 | 413 |
Labelled Cell Count | 791 | 193 | 353 | 474 | 632 | 656 | 763 | 740 | 702 | 692 | 438 | 465 | 278 | 340 | 351 | 218 | 273 | 267 | 744 | 190 | 360 | 230 | 373 | 413 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 7.3 | 6.6 | 6.0 | 6.0 | 5.3 | 5.4 | 6.0 | 5.3 | 5.7 | 5.0 | 5.7 | 6.8 | 7.4 | 7.3 | 8.9 | 8.8 | 9.0 | 6.7 | 6.6 | 7.7 |
Std Deviation (1e-4) | 1.0 | 1.6 | 1.2 | 1.6 | 1.5 | 1.4 | 1.4 | 1.4 | 1.4 | 1.0 | 1.3 | 1.1 | 1.6 | 2.1 | 1.7 | 2.5 | 1.7 | 2.3 | 1.8 | 1.9 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.14 | -0.3 | -0.28 | -0.14 | -0.3 | -0.22 | -0.41 | -0.21 | 0.04 | 0.16 | 0.15 | 0.43 | 0.42 | 0.45 | 0.02 | 0.0 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.6 | -2.0 | -4.9 | -7.0 | -6.4 | -9.0 | -7.8 | -8.6 | -7.5 | -0.8 | 1.2 | 2.1 | -10.2 | -10.1 | -8.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.6 | 6.5 | 5.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.1 | 3.5 | 7.2 | 9.0 | 7.7 | 12.1 | 8.6 | 14.0 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.5 | -1.6 | 0.6 | 0.5 | 1.2 | 0.1 | 1.1 | 1.2 | 2.2 | 0 | 0 | 0 | 1.9 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 |
Vacuole | 3.9 | 2.4 | 2.5 | 2.8 | 3.3 | 2.9 | 2.8 | 0.1 | 2.4 | 2.0 | 3.2 | 0.6 | 8.0 | 9.5 | 7.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5962 | 2.9678 | 2.767 | 2.2605 | 2.2169 | 2.8108 | 1.696 | 1.9894 | 2.0008 | 2.4982 | 1.8029 | 1.8832 | 2.4267 | 2.0337 | 2.2378 | 1.9698 | 2.1389 | 1.7919 |
Actin | 0.0183 | 0.0012 | 0.0031 | 0.0011 | 0.0054 | 0.0064 | 0.0159 | 0.0009 | 0.0149 | 0.0225 | 0.0201 | 0.0014 | 0.0399 | 0.0018 | 0.0078 | 0.0435 | 0.0144 | 0.0008 |
Bud | 0.0006 | 0.0008 | 0.0012 | 0.0019 | 0.0002 | 0.0005 | 0.001 | 0.0006 | 0.0005 | 0.0038 | 0.003 | 0.0017 | 0.001 | 0.0054 | 0.0035 | 0.001 | 0.0026 | 0.0034 |
Bud Neck | 0.0104 | 0.0007 | 0.0016 | 0.0014 | 0.002 | 0.0073 | 0.0064 | 0.0016 | 0.0046 | 0.0262 | 0.0043 | 0.0036 | 0.0086 | 0.0004 | 0.0056 | 0.0029 | 0.0011 | 0.0024 |
Bud Periphery | 0.0014 | 0.0007 | 0.0017 | 0.0053 | 0.0001 | 0.0005 | 0.0011 | 0.0003 | 0.0005 | 0.0043 | 0.0048 | 0.001 | 0.0011 | 0.006 | 0.0011 | 0.0007 | 0.0049 | 0.0022 |
Bud Site | 0.0057 | 0.0038 | 0.0034 | 0.0012 | 0.0003 | 0.0005 | 0.0064 | 0.0104 | 0.0031 | 0.0509 | 0.0095 | 0.0005 | 0.0073 | 0.0078 | 0.0022 | 0.0014 | 0.0036 | 0.0004 |
Cell Periphery | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.5112 | 0.6511 | 0.5117 | 0.6303 | 0.5954 | 0.5997 | 0.5713 | 0.5921 | 0.5606 | 0.513 | 0.4805 | 0.6112 | 0.5692 | 0.7962 | 0.6883 | 0.6609 | 0.5735 | 0.7265 |
Cytoplasmic Foci | 0.0181 | 0.0108 | 0.0178 | 0.0097 | 0.0111 | 0.0159 | 0.0189 | 0.0087 | 0.0124 | 0.0289 | 0.0225 | 0.0136 | 0.0254 | 0.0087 | 0.0097 | 0.0125 | 0.0344 | 0.0087 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0008 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.006 | 0.0033 | 0.0037 | 0.0046 | 0.0039 | 0.0046 | 0.0044 | 0.0023 | 0.0033 | 0.0082 | 0.0054 | 0.0021 | 0.0063 | 0.0033 | 0.0062 | 0.0043 | 0.0055 | 0.0026 |
Endosome | 0.0145 | 0.0059 | 0.0086 | 0.0058 | 0.0095 | 0.014 | 0.0132 | 0.0031 | 0.0096 | 0.0242 | 0.0195 | 0.0049 | 0.0344 | 0.0028 | 0.0108 | 0.0301 | 0.0543 | 0.0041 |
Golgi | 0.0027 | 0.0003 | 0.0013 | 0.0003 | 0.0007 | 0.0013 | 0.0027 | 0.0003 | 0.0028 | 0.0044 | 0.0049 | 0.0011 | 0.01 | 0.0004 | 0.0017 | 0.0096 | 0.0118 | 0.0003 |
Lipid Particles | 0.0082 | 0.0002 | 0.001 | 0.0003 | 0.0009 | 0.0009 | 0.004 | 0.0001 | 0.0027 | 0.0079 | 0.007 | 0.0003 | 0.0173 | 0.0007 | 0.001 | 0.0108 | 0.0095 | 0.0001 |
Mitochondria | 0.0057 | 0.0006 | 0.0011 | 0.0022 | 0.0007 | 0.0012 | 0.0041 | 0.0006 | 0.0056 | 0.0024 | 0.0012 | 0.0014 | 0.0059 | 0.0007 | 0.0102 | 0.0108 | 0.0119 | 0.0014 |
None | 0.2865 | 0.209 | 0.3541 | 0.268 | 0.1849 | 0.2301 | 0.1969 | 0.297 | 0.2307 | 0.1066 | 0.3201 | 0.182 | 0.1573 | 0.091 | 0.1551 | 0.0772 | 0.1648 | 0.1239 |
Nuclear Periphery | 0.0104 | 0.0056 | 0.0061 | 0.007 | 0.0099 | 0.0086 | 0.0082 | 0.0036 | 0.0078 | 0.0197 | 0.0175 | 0.0059 | 0.0122 | 0.0036 | 0.0158 | 0.0065 | 0.0311 | 0.0046 |
Nucleolus | 0.0011 | 0.0005 | 0.0007 | 0.0004 | 0.0008 | 0.001 | 0.001 | 0.0008 | 0.0015 | 0.0047 | 0.0008 | 0.0009 | 0.0038 | 0.0007 | 0.0009 | 0.0004 | 0.0016 | 0.0003 |
Nucleus | 0.078 | 0.0965 | 0.0617 | 0.0503 | 0.1397 | 0.0916 | 0.1146 | 0.0669 | 0.1146 | 0.072 | 0.0453 | 0.1368 | 0.0615 | 0.0628 | 0.0673 | 0.0771 | 0.0459 | 0.1104 |
Peroxisomes | 0.009 | 0.0003 | 0.0074 | 0.0008 | 0.0007 | 0.0008 | 0.0139 | 0.0005 | 0.0097 | 0.0149 | 0.0057 | 0.0019 | 0.0149 | 0.0006 | 0.0006 | 0.0397 | 0.0102 | 0.0005 |
Punctate Nuclear | 0.0065 | 0.0062 | 0.0112 | 0.0075 | 0.0318 | 0.0123 | 0.0127 | 0.0084 | 0.0127 | 0.081 | 0.0256 | 0.0279 | 0.0144 | 0.0048 | 0.0075 | 0.0035 | 0.0097 | 0.0057 |
Vacuole | 0.0038 | 0.0021 | 0.0019 | 0.0014 | 0.0013 | 0.0018 | 0.0026 | 0.0016 | 0.0015 | 0.0022 | 0.0015 | 0.0014 | 0.0063 | 0.002 | 0.0034 | 0.0036 | 0.005 | 0.0014 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0005 | 0.0008 | 0.0005 | 0.0003 | 0.0024 | 0.0003 | 0.001 | 0.0023 | 0.0038 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.0296 | 44.3608 | 41.9539 | 70.6331 | 56.1395 | 57.3764 | 58.0453 | 63.2169 | 69.5607 | 63.4217 |
Translational Efficiency | 1.1744 | 1.2224 | 1.4098 | 0.9739 | 1.0601 | 1.2101 | 1.1561 | 1.0379 | 0.9675 | 0.9399 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
55 | 312 | 2469 | 1787 | 1430 | 2865 | 238 | 1253 | 1485 | 3177 | 2707 | 3040 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 842.22 | 766.65 | 946.21 | 1047.61 | 656.90 | 791.91 | 1123.38 | 1136.66 | 663.76 | 789.43 | 961.79 | 1084.31 |
Standard Deviation | 130.82 | 160.66 | 140.73 | 164.77 | 81.45 | 114.65 | 157.93 | 187.92 | 90.81 | 120.19 | 150.91 | 180.10 |
Intensity Change Log 2 | — | -0.135629 | 0.167963 | 0.314833 | — | 0.269663 | 0.774100 | 0.791055 | — | 0.056098 | 0.465229 | 0.543035 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001275 | 0.000524 | 0.000227 | 0.000891 | 0.000219 | 0.000180 | 0.001448 | 0.004424 | 0.000258 | 0.000213 | 0.000334 | 0.002347 |
Bud Neck | 0.046142 | 0.015403 | 0.004339 | 0.013485 | 0.007113 | 0.011961 | 0.004306 | 0.011718 | 0.008559 | 0.012299 | 0.004336 | 0.012757 |
Bud Site | 0.047547 | 0.014210 | 0.004582 | 0.027230 | 0.002803 | 0.006574 | 0.004178 | 0.049876 | 0.004460 | 0.007323 | 0.004547 | 0.036564 |
Cell Periphery | 0.000461 | 0.000143 | 0.000105 | 0.000261 | 0.000100 | 0.000066 | 0.000264 | 0.000178 | 0.000114 | 0.000074 | 0.000119 | 0.000227 |
Cytoplasm | 0.341858* | 0.458716* | 0.288406* | 0.268894* | 0.533266* | 0.629083* | 0.112865* | 0.116632* | 0.526177* | 0.612352* | 0.272972* | 0.206136* |
Cytoplasmic Foci | 0.110850 | 0.170446 | 0.001209 | 0.011899 | 0.121581 | 0.049496 | 0.001754 | 0.042884 | 0.121183 | 0.061374 | 0.001257 | 0.024670 |
Eisosomes | 0.000129 | 0.000084 | 0.000013 | 0.000023 | 0.000060 | 0.000024 | 0.000064 | 0.000020 | 0.000063 | 0.000030 | 0.000018 | 0.000022 |
Endoplasmic Reticulum | 0.009495 | 0.002394 | 0.002232 | 0.004560 | 0.000859 | 0.000810 | 0.014128 | 0.001551 | 0.001179 | 0.000966 | 0.003278 | 0.003320 |
Endosome | 0.014514 | 0.011559 | 0.000500 | 0.003010 | 0.004422 | 0.003240 | 0.002449 | 0.014588 | 0.004796 | 0.004057 | 0.000672 | 0.007782 |
Golgi | 0.003237 | 0.001571 | 0.000047 | 0.007387 | 0.000560 | 0.001035 | 0.000160 | 0.016305 | 0.000660 | 0.001088 | 0.000057 | 0.011063 |
Lipid Particles | 0.026403 | 0.006257 | 0.000312 | 0.001159 | 0.003929 | 0.001138 | 0.002237 | 0.001131 | 0.004761 | 0.001641 | 0.000481 | 0.001147 |
Mitochondria | 0.003122 | 0.002921 | 0.000926 | 0.002721 | 0.002463 | 0.003910 | 0.001048 | 0.012155 | 0.002488 | 0.003813 | 0.000937 | 0.006609 |
Mitotic Spindle | 0.018990 | 0.005379 | 0.006774 | 0.157791* | 0.001285 | 0.011655 | 0.025677 | 0.596434* | 0.001941 | 0.011039 | 0.008436 | 0.338587* |
None | 0.002393 | 0.004094 | 0.006931 | 0.009337 | 0.004725 | 0.005616 | 0.016368 | 0.004039 | 0.004639 | 0.005466 | 0.007761 | 0.007153 |
Nuclear Periphery | 0.001819 | 0.000638 | 0.001958 | 0.003088 | 0.000653 | 0.000564 | 0.002995 | 0.001157 | 0.000696 | 0.000571 | 0.002049 | 0.002292 |
Nuclear Periphery Foci | 0.020010 | 0.001781 | 0.000403 | 0.004522 | 0.000718 | 0.000490 | 0.015226 | 0.002554 | 0.001432 | 0.000616 | 0.001706 | 0.003710 |
Nucleolus | 0.007094 | 0.002433 | 0.000292 | 0.001941 | 0.001132 | 0.001087 | 0.000850 | 0.003573 | 0.001353 | 0.001219 | 0.000341 | 0.002614 |
Nucleus | 0.224386* | 0.197259* | 0.664460* | 0.428000* | 0.276979* | 0.241889* | 0.699060* | 0.076816 | 0.275031* | 0.237506* | 0.667502* | 0.283252* |
Peroxisomes | 0.014713 | 0.012477 | 0.000043 | 0.001389 | 0.003214 | 0.002218 | 0.000195 | 0.006320 | 0.003640 | 0.003226 | 0.000057 | 0.003421 |
Vacuole | 0.099337 | 0.090562 | 0.016163 | 0.044439 | 0.033535 | 0.028155 | 0.094549 | 0.033206 | 0.035973 | 0.034284 | 0.023055 | 0.039809 |
Vacuole Periphery | 0.006226 | 0.001149 | 0.000077 | 0.007973 | 0.000382 | 0.000809 | 0.000180 | 0.004439 | 0.000599 | 0.000843 | 0.000087 | 0.006517 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.92 | 2.67 | 0.78 | -3.58 | -8.09 | 0.34 | -9.84 | -2.36 | -2.47 | 0.60 | 0.36 | -2.01 | -3.90 | -4.35 | -3.33 |
Bud Neck | 1.61 | 2.08 | 1.14 | -3.78 | -13.51 | -4.37 | -0.96 | -11.97 | -8.85 | -11.29 | -3.06 | 1.65 | -11.95 | -10.37 | -18.34 |
Bud Site | 1.87 | 2.41 | 1.24 | -3.68 | -10.74 | -4.98 | -3.12 | -16.79 | -15.34 | -14.51 | -2.87 | -0.62 | -17.82 | -16.92 | -18.18 |
Cell Periphery | 4.11 | 4.80 | 3.24 | -2.99 | -9.65 | 6.28 | -8.63 | -7.96 | -11.41 | 2.76 | 6.17 | -0.64 | -9.99 | -16.16 | -10.00 |
Cytoplasm | -2.37 | 2.60 | 3.90 | 15.76 | 8.14 | -10.86 | 46.58 | 51.35 | 75.91* | -2.44 | -9.74 | 37.43 | 48.75 | 76.80* | 13.44 |
Cytoplasmic Foci | -2.59 | 5.23 | 4.54 | 14.22 | -9.96 | 16.07 | 27.36 | 16.52 | 3.14 | -10.75 | 13.25 | 30.04 | 22.62 | 14.20 | -15.75 |
Eisosomes | 2.18 | 6.13 | 5.45 | 8.40 | -7.99 | 13.66 | -4.37 | 8.09 | -5.76 | 8.14 | 11.99 | 14.13 | 11.15 | -2.37 | -5.23 |
Endoplasmic Reticulum | 3.24 | 2.94 | 2.40 | -3.00 | -3.86 | 0.53 | -14.34 | -7.79 | -7.65 | 12.64 | 1.24 | -17.62 | -12.97 | -13.89 | 4.12 |
Endosome | 0.84 | 4.81 | 4.16 | 7.27 | -10.27 | 2.42 | 3.27 | -1.32 | -3.56 | -4.32 | 1.63 | 8.63 | 2.18 | 0.55 | -10.88 |
Golgi | 1.21 | 2.31 | 0.80 | -0.57 | -2.54 | -1.45 | 2.81 | -3.08 | -2.53 | -3.50 | -1.35 | 4.63 | -3.18 | -2.41 | -4.29 |
Lipid Particles | 1.61 | 2.11 | 1.99 | 4.43 | -11.38 | 6.55 | 3.84 | 3.30 | -5.14 | -0.78 | 4.97 | 6.81 | 4.31 | -2.39 | -12.27 |
Mitochondria | 0.24 | 1.95 | 0.68 | 0.53 | -7.69 | -1.39 | 1.42 | -0.57 | 0.62 | -2.02 | -1.33 | 1.66 | -0.27 | 1.59 | -3.91 |
Mitotic Spindle | 0.70 | 0.68 | -10.97 | -23.09* | -24.39* | -6.32 | -2.18 | -42.72* | -41.51* | -37.68* | -5.77 | -4.48 | -44.72 | -42.85* | -43.48* |
None | -1.55 | -5.72 | -11.07 | -9.14 | -9.63 | -1.08 | -1.62 | -2.49 | -1.57 | 0.48 | -1.04 | -3.13 | -7.52 | -7.57 | -7.19 |
Nuclear Periphery | 1.07 | -1.09 | -3.27 | -20.86 | -10.47 | 1.36 | -12.52 | -14.58 | -15.15 | 0.93 | 1.61 | -23.70 | -24.82 | -26.94 | -9.31 |
Nuclear Periphery Foci | 1.64 | 1.78 | 0.70 | -7.74 | -9.74 | 1.57 | -2.43 | -7.35 | -7.86 | -1.53 | 2.05 | 0.80 | -9.90 | -12.39 | -11.43 |
Nucleolus | 1.79 | 2.59 | -1.10 | -7.21 | -10.40 | -0.11 | 0.61 | -9.39 | -9.29 | -9.42 | 0.25 | 4.31 | -12.67 | -12.73 | -14.37 |
Nucleus | 0.84 | -14.46 | -5.89 | -15.54 | 29.61 | 4.15 | -22.69 | 13.96 | 12.18 | 29.33 | 4.67 | -53.39 | -3.87 | -9.44 | 50.55 |
Peroxisomes | 0.28 | 1.79 | 1.64 | 4.66 | -7.51 | 1.51 | 4.45 | -0.80 | -3.40 | -8.37 | 0.70 | 5.02 | 1.78 | 1.98 | -11.13 |
Vacuole | 0.07 | 3.22 | 1.20 | 2.66 | -10.63 | 1.90 | -8.41 | -9.71 | -11.14 | 4.63 | 0.04 | -2.98 | -12.35 | -13.75 | -9.62 |
Vacuole Periphery | 2.11 | 2.53 | 1.75 | -0.99 | -2.40 | -2.19 | 4.87 | -2.12 | -0.40 | -2.87 | -1.14 | 5.05 | -1.89 | -1.34 | -3.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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