Standard name
Human Ortholog
Description Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.27 0.11 0.11 0.05 0.08 0.05 0 0.07 0.07 0 0 0 0.06 0.05 0 0.37 0.26 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.16 0.11 0.2 0.19 0.25 0.46 0.46 0.36 0.38 0.4 0.4 0.37 0.07 0 0.06 0.08 0.07 – 0 0 0 0 0 0
Nucleus 0.89 0.86 0.88 0.9 0.79 0.72 0.69 0.63 0.65 0.69 0.68 0.66 0.91 0.88 0.83 0.72 0.78 – 0.83 0.86 0.82 0.81 0.75 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0
Nucleolus 0.05 0.16 0.14 0.21 0.29 0.49 0.48 0.5 0.5 0.56 0.54 0.56 0.13 0.12 0.15 0.1 0.06 – 0 0 0.05 0.06 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 4 0 3 2 4 4
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 2 2 2 1 0 0 2 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 70 12 41 14 27 30 33 45 44 18 13 13 22 22 11 34 21 – 3 0 1 1 3 3
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 7 – 0 0 0 1 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 – 0 0 0 0 1 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 1 0 0 0
Mitochondria 42 12 76 50 83 260 359 249 223 311 225 260 27 14 25 7 6 – 0 0 1 1 2 2
Nucleus 232 96 328 242 259 401 536 433 380 533 389 458 361 366 336 66 63 – 221 91 295 292 303 268
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – 7 5 15 9 10 10
Nucleolus 13 18 53 56 97 274 369 343 296 432 309 388 50 50 61 9 5 – 9 3 17 20 30 40
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 4 7 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 3 1 6 3 3 0 2 13 2 2 1 3 1 6 10 1 1 – 3 1 7 11 25 30
Unique Cell Count 261 112 371 269 329 560 774 690 588 778 568 695 396 415 404 92 81 267 106 362 359 406 385
Labelled Cell Count 360 140 506 365 469 965 1300 1084 947 1299 939 1123 464 463 454 120 103 267 106 362 359 406 385


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 172.8 182.3 151.4 135.4 106.0 86.6 73.9 70.9 61.9 53.2 47.6 44.1 155.9 153.8 140.1 240.2 218.5 – 143.2 151.0 152.6
Std Deviation (1e-4) 57.8 75.3 48.5 42.6 37.4 33.5 31.2 32.0 26.9 20.4 20.0 18.4 49.4 52.8 48.9 86.6 85.1 – 50.1 53.1 58.3
Intensity Change (Log2) – – – -0.16 -0.51 -0.81 -1.04 -1.09 -1.29 -1.51 -1.67 -1.78 0.04 0.02 -0.11 0.67 0.53 – -0.08 -0.0 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm -2.6 -1.3 -3.2 -4.4 -2.6 -1.9 -6.3 -5.6 -6.5 -2.8 -3.0 -4.6 6.0 3.5 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria -0.6 1.5 8.1 8.4 5.3 5.7 6.5 6.1 5.7 -5.5 -7.5 -5.9 -2.9 -2.8 –
Nucleus 0.6 -3.5 -6.1 -7.1 -8.8 -8.2 -7.3 -7.0 -8.0 1.3 -0.1 -2.1 -4.0 -2.5 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 2.2 4.9 10.8 11.0 11.4 11.3 13.2 12.3 13.1 -0.7 -0.9 0.3 -1.1 -2.0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 0.3 0 0 0 0 0 -0.2 0.8 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 488.6892 451.4349 437.024 425.9124 417.7193 425.1257 457.8695 526.9491 506.2605 507.4828 465.8065 462.6642 566.2144 562.1984 520.4659 536.2219 461.3776 531.7499
Actin 0 0 0 0 0 0 0.0294 0 0.002 0 0 0.0103 0.0067 0 0.0012 0 0.0034 0
Bud 0.0001 0 0 0 0 0 0.0008 0 0.0003 0 0 0.0012 0.0007 0 0.0014 0 0.0011 0
Bud Neck 0 0 0 0 0 0 0.0015 0 0.0004 0 0 0.0006 0.0006 0 0.0002 0 0.0007 0
Bud Periphery 0.0001 0 0 0 0 0 0.0013 0 0.0006 0 0 0.0062 0.0008 0 0.0053 0 0.0092 0
Bud Site 0.0001 0 0 0 0 0 0.0031 0 0.0008 0 0 0.0006 0.0027 0 0.0009 0 0.0009 0
Cell Periphery 0.001 0 0 0 0.0001 0.0003 0.0022 0.0001 0.0003 0 0.0002 0.0023 0.0018 0.0001 0.0006 0.0001 0.0038 0.0001
Cytoplasm 0.0001 0 0 0 0 0 0.0087 0 0.0002 0 0 0.0016 0.0074 0 0.0003 0 0.0003 0
Cytoplasmic Foci 0.0003 0 0 0 0 0 0.0162 0 0.0026 0 0 0.0009 0.0015 0 0.0024 0 0.0032 0
Eisosomes 0.0001 0 0 0 0 0 0.0022 0 0 0 0 0.0004 0.0001 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0053 0 0.0001 0 0 0.0014 0.0025 0 0.0009 0 0.0004 0
Endosome 0 0 0 0 0 0 0.015 0 0.0029 0 0 0.0125 0.0072 0 0.001 0 0.0019 0
Golgi 0 0 0 0 0 0 0.0029 0 0.0011 0 0 0.0031 0.0013 0 0.0007 0 0.001 0
Lipid Particles 0.0003 0 0 0 0 0 0.0049 0 0.0036 0 0 0.0013 0.0014 0 0.0003 0 0.0012 0
Mitochondria 0.0045 0.0012 0.0038 0.0041 0.0089 0.0018 0.0172 0.003 0.0103 0.0016 0.0172 0.0159 0.0058 0.0042 0.0133 0.0038 0.0187 0.0015
None 0.0001 0 0 0 0 0 0.0078 0 0.0004 0 0 0.0047 0.0014 0 0.0012 0 0.0002 0
Nuclear Periphery 0.0001 0 0 0.0001 0 0 0.0154 0.0026 0.0006 0.0001 0 0.0137 0.0176 0.0012 0.0011 0.0005 0.0006 0
Nucleolus 0.0746 0.0333 0.0458 0.058 0.1894 0.0836 0.1011 0.0396 0.0466 0.03 0.294 0.0699 0.0684 0.044 0.0559 0.0884 0.2691 0.0789
Nucleus 0.917 0.9653 0.9499 0.9374 0.8009 0.9136 0.7429 0.9513 0.9121 0.9682 0.6876 0.8469 0.8539 0.9489 0.904 0.9048 0.6749 0.9189
Peroxisomes 0.0001 0 0 0 0 0 0.0087 0 0.0098 0 0 0.0006 0.0007 0 0.0002 0 0.0005 0
Punctate Nuclear 0 0 0 0 0 0 0.0067 0 0.0006 0 0 0.0019 0.0131 0 0.0059 0 0.0001 0
Vacuole 0.0009 0 0 0.0001 0.0001 0.0002 0.0043 0.0004 0.0012 0 0.0001 0.002 0.0028 0.0002 0.0015 0.0003 0.0062 0.0001
Vacuole Periphery 0.0007 0.0001 0.0003 0.0003 0.0005 0.0003 0.0025 0.003 0.0037 0.0001 0.0008 0.0022 0.0016 0.0013 0.0013 0.002 0.0025 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1129.9822 1189.4762 1166.4304 1080.6605 893.1599 1422.4067 1485.7182 1291.5973 1301.1755 1140.4771
Translational Efficiency 1.0265 1.0302 0.9834 1.0794 1.0491 1.0571 1.066 1.134 0.9857 1.0678

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2047 1439 601 104 2008 1736 1260 1619 4055 3175 1861 1723

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 9962.75 12670.00 13164.42 13013.81 9590.02 12214.59 10247.89 10152.60 9778.18 12420.99 11189.77 10325.30
Standard Deviation 3230.15 2944.66 2471.81 2764.73 2717.76 2954.64 2572.08 3039.78 2993.23 2958.82 2883.08 3099.71
Intensity Change Log 2 — 0.346801 0.402028 0.385427 — 0.349000 0.095721 0.082244 — 0.347880 0.259894 0.244661

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006483 0.017944 0.010557 0.005884 0.004598 0.015892 0.004141 0.003002 0.005549 0.016822 0.006213 0.003175
Bud Neck 0.000888 0.001213 0.002416 0.003403 0.001181 0.001536 0.003102 0.003484 0.001033 0.001390 0.002881 0.003480
Bud Site 0.000076 0.000098 0.000117 0.000162 0.000108 0.000139 0.000212 0.000434 0.000092 0.000121 0.000181 0.000417
Cell Periphery 0.000443 0.000354 0.000068 0.000036 0.000342 0.000424 0.000070 0.000434 0.000393 0.000392 0.000070 0.000410
Cytoplasm 0.000101 0.000477 0.000025 0.000023 0.000016 0.000061 0.000044 0.000873 0.000059 0.000249 0.000038 0.000821
Cytoplasmic Foci 0.000229 0.000941 0.000214 0.000312 0.000406 0.000607 0.000698 0.002117 0.000317 0.000758 0.000541 0.002008
Eisosomes 0.009822 0.010326 0.000975 0.000454 0.009275 0.010618 0.001464 0.000643 0.009551 0.010486 0.001306 0.000631
Endoplasmic Reticulum 0.001629 0.005179 0.002134 0.000595 0.001172 0.004230 0.000798 0.000823 0.001403 0.004660 0.001230 0.000810
Endosome 0.000002 0.000009 0.000006 0.000004 0.000002 0.000110 0.000002 0.000241 0.000002 0.000064 0.000003 0.000227
Golgi 0.000003 0.000014 0.000025 0.000024 0.000003 0.000029 0.000008 0.000676 0.000003 0.000022 0.000013 0.000637
Lipid Particles 0.000006 0.000051 0.000011 0.000009 0.000003 0.000335 0.000003 0.000011 0.000005 0.000207 0.000006 0.000011
Mitochondria 0.001552 0.003987 0.007321 0.008173 0.001288 0.003335 0.002844 0.003752 0.001421 0.003630 0.004290 0.004019
Mitotic Spindle 0.000000 0.000003 0.000004 0.000004 0.000000 0.000026 0.000012 0.000949 0.000000 0.000015 0.000010 0.000892
None 0.000772 0.001213 0.000432 0.000480 0.000595 0.001055 0.000345 0.000883 0.000684 0.001127 0.000373 0.000858
Nuclear Periphery 0.003340 0.047026 0.062938 0.015779 0.001272 0.016817 0.002376 0.003251 0.002316 0.030509 0.021934 0.004007
Nuclear Periphery Foci 0.000060 0.000775 0.000637 0.000248 0.000020 0.000290 0.000017 0.000048 0.000040 0.000510 0.000217 0.000060
Nucleolus 0.126015 0.378835* 0.425297* 0.230709* 0.113747 0.330748* 0.145271 0.062577 0.119940 0.352542* 0.235704* 0.072725
Nucleus 0.803405* 0.329440* 0.429353* 0.703276* 0.842097* 0.505375* 0.824617* 0.901633* 0.822565* 0.425636* 0.696968* 0.889660*
Peroxisomes 0.003053 0.010858 0.020267 0.011064 0.003556 0.011261 0.007873 0.003465 0.003302 0.011078 0.011876 0.003924
Vacuole 0.028323 0.102669 0.011631 0.003566 0.013228 0.057406 0.002959 0.005426 0.020848 0.077920 0.005760 0.005314
Vacuole Periphery 0.013800 0.088588 0.025574 0.015797 0.007091 0.039708 0.003143 0.005278 0.010478 0.061862 0.010387 0.005913

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -15.37 -5.28 0.66 10.56 4.04 -16.73* 1.29 5.07 20.01* 3.72 -22.47* -1.82 8.99 27.82* 8.92
Bud Neck -3.97 -8.67 -6.92 -5.97 -2.52 -3.91 -13.93 -13.54 -10.80 -2.13 -5.76 -17.12 -15.52 -12.82 -3.52
Bud Site -1.21 -3.22 -2.77 -0.38 -1.44 -1.44 -7.35 -2.95 -2.47 -2.03 -1.87 -8.72 -3.13 -2.52 -2.29
Cell Periphery 0.68 3.56 3.86 5.67 3.16 -0.94 6.09 -0.73 -0.08 -2.91 -0.06 5.61 -0.16 -0.13 -2.91
Cytoplasm -1.33 1.93 2.06 1.48 0.13 -2.95 -1.42 -1.72 -1.62 -1.66 -1.42 0.93 -1.63 -0.85 -1.67
Cytoplasmic Foci -1.48 0.43 -1.07 1.25 -1.17 -1.03 -0.67 -2.53 -2.21 -1.80 -1.87 -0.77 -2.72 -1.95 -2.14
Eisosomes -0.47 12.27 13.04 12.40 3.30 -1.08 8.76 9.65 11.42 4.40 -1.13 14.38 15.36 16.26 4.21
Endoplasmic Reticulum -9.50 -1.40 6.28 12.39 4.37 -10.41 2.79 2.32 11.78 -0.19 -13.94 1.22 4.69 16.16 2.73
Endosome -8.10 -5.91 -2.94 5.17 2.36 -1.05 -2.14 -1.91 -0.79 -1.90 -1.11 -5.49 -1.90 -1.23 -1.89
Golgi -4.19 -9.53 -5.30 -2.09 0.12 -1.42 -4.22 -1.45 -1.39 -1.44 -1.90 -9.98 -1.45 -1.41 -1.43
Lipid Particles -15.54 -4.07 -1.22 13.63 1.33 -1.06 0.30 -1.49 1.04 -1.51 -1.18 -1.34 -1.24 1.14 -1.12
Mitochondria -11.54 -14.77 -6.77 -4.18 -0.78 -13.63 -11.85 -5.51 -0.91 -1.99 -17.49 -17.77 -6.12 -0.90 0.59
Mitotic Spindle -1.67 -7.81 -2.54 -0.74 -0.09 -2.54 -1.10 -2.18 -2.12 -2.15 -2.73 -1.26 -2.18 -2.14 -2.15
None -2.62 4.56 3.53 4.58 -0.95 -2.08 1.75 -1.58 0.75 -4.19 -3.15 3.91 -1.30 1.51 -4.04
Nuclear Periphery -13.10 -8.85 -1.82 4.24 5.00 -9.65 -1.63 -2.81 7.97 -0.99 -15.89 -8.43 -2.03 14.13 7.46
Nuclear Periphery Foci -10.97 -7.48 -2.28 5.18 3.54 -4.79 0.63 -1.96 4.12 -2.18 -10.92 -6.73 -1.35 9.92 5.22
Nucleolus -24.77* -20.78* -3.52 5.06 6.24 -22.86* -3.50 8.04 30.33* 10.17 -33.41* -14.30 8.67 38.97* 19.75
Nucleus 42.76* 22.95 2.87 -10.96 -7.57 31.81 1.70 -7.65 -39.05 -8.17 50.66* 13.24 -9.81 -55.43 -19.44
Peroxisomes -14.64 -12.70 -4.24 -0.17 3.88 -8.59 -6.53 0.09 8.06 5.83 -14.24 -13.55 -1.47 10.55 10.58
Vacuole -14.90 6.65 12.32 21.12 4.33 -13.29 8.25 5.28 16.03 -2.52 -19.36 11.85 11.13 25.39 0.37
Vacuole Periphery -20.48* -4.71 -0.37 12.69 1.95 -16.01 4.57 1.75 17.09 -2.58 -25.07 0.13 4.58 27.14 4.13
Standard name
Human Ortholog
Description Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (88%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Lys20

Lys20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available