Standard name | |
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Human Ortholog | |
Description | RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 1 | 4 | 1 | 4 | 3 | 5 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 3 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | 19 | 9 | 13 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 7 | 0 | 9 | 16 | 6 | 14 | 24 | 16 | 19 | 11 | 8 | 7 | 19 | 37 | 4 | 7 | 14 | 0 | 0 | 0 | 3 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 4 | 3 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 31 | 8 | 2 | 9 | 4 | 62 | 110 | 96 | 213 | 175 | 213 | 144 | 3 | 2 | 1 | 3 | 4 | 6 | 5 | 0 | 0 | 0 | 4 | 8 |
Nucleus | 294 | 105 | 55 | 153 | 284 | 230 | 305 | 304 | 323 | 434 | 325 | 336 | 205 | 220 | 198 | 100 | 88 | 99 | 281 | 102 | 77 | 147 | 178 | 167 |
Nuclear Periphery | 4 | 1 | 0 | 0 | 3 | 2 | 3 | 6 | 3 | 4 | 7 | 3 | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 |
Nucleolus | 2 | 2 | 0 | 3 | 0 | 1 | 5 | 10 | 13 | 12 | 26 | 21 | 5 | 1 | 4 | 4 | 6 | 2 | 4 | 0 | 0 | 11 | 22 | 35 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 6 | 2 | 1 | 1 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 6 | 5 |
Vac/Vac Membrane | 2 | 2 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 7 | 4 | 6 | 2 | 1 | 5 | 2 | 6 | 8 | 6 | 3 | 5 | 18 | 59 | 76 |
Unique Cell Count | 310 | 118 | 57 | 164 | 303 | 253 | 342 | 364 | 412 | 515 | 386 | 403 | 215 | 243 | 245 | 107 | 103 | 126 | 314 | 114 | 87 | 192 | 286 | 321 |
Labelled Cell Count | 340 | 126 | 57 | 176 | 312 | 308 | 449 | 457 | 594 | 664 | 605 | 535 | 224 | 250 | 251 | 117 | 113 | 132 | 314 | 114 | 87 | 192 | 286 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.0 | 5.3 | 5.7 | 5.8 | 4.7 | 4.4 | 5.0 | 4.2 | 4.5 | 4.1 | 4.5 | 6.3 | 6.6 | 6.3 | 8.3 | 9.0 | 9.2 | 5.6 | 6.1 | 6.5 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.6 | 1.6 | 1.5 | 1.2 | 1.1 | 1.6 | 0.9 | 1.2 | 1.0 | 0.9 | 1.1 | 1.4 | 1.4 | 1.7 | 2.0 | 2.0 | 1.3 | 1.6 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.14 | -0.17 | -0.28 | -0.08 | -0.35 | -0.23 | -0.36 | -0.23 | 0.26 | 0.33 | 0.24 | 0.64 | 0.76 | 0.79 | 0.09 | 0.22 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.9 | -0.8 | -1.4 | -1.7 | -2.6 | -3.2 | -2.5 | -2.5 | -2.6 | -0.4 | -1.5 | -2.9 | -0.8 | -2.2 | -3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7817 | 2.9134 | 2.6315 | 2.5495 | 2.9027 | 2.5433 | 2.0906 | 2.4186 | 2.1561 | 1.593 | 1.6601 | 1.9728 | 1.1219 | 1.1046 | 0.801 | 0.952 | 0.5929 | 0.7886 |
Actin | 0.0271 | 0.0001 | 0.0143 | 0.016 | 0.0116 | 0.0012 | 0.0109 | 0 | 0.0046 | 0 | 0.0045 | 0.0002 | 0.0636 | 0.0002 | 0.0057 | 0.016 | 0.0269 | 0.0017 |
Bud | 0.0005 | 0 | 0.0002 | 0.0017 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0083 | 0.0002 | 0.0002 |
Bud Neck | 0.0012 | 0.0002 | 0.0007 | 0.0009 | 0.001 | 0.0021 | 0.0011 | 0.0001 | 0.0007 | 0.0001 | 0.0032 | 0.0012 | 0.0039 | 0.0002 | 0.0005 | 0.0013 | 0.0008 | 0.0012 |
Bud Periphery | 0.0006 | 0.0001 | 0.0004 | 0.0025 | 0.0073 | 0.0007 | 0.0002 | 0 | 0.0002 | 0 | 0.0008 | 0.0003 | 0.0025 | 0.0005 | 0.0005 | 0.0401 | 0.0003 | 0.0005 |
Bud Site | 0.0013 | 0.0002 | 0.001 | 0.0117 | 0.0009 | 0.0003 | 0.0006 | 0 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.0124 | 0.0004 | 0.0124 | 0.0065 | 0.0009 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0018 | 0.0001 | 0.0001 |
Cytoplasm | 0.0096 | 0.0051 | 0.0072 | 0.0021 | 0.002 | 0.0008 | 0.0023 | 0.0001 | 0.0007 | 0.0001 | 0.0015 | 0.0001 | 0.001 | 0.001 | 0.0008 | 0.0016 | 0.0661 | 0.0001 |
Cytoplasmic Foci | 0.0129 | 0.0004 | 0.0054 | 0.0105 | 0.0095 | 0.001 | 0.009 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0046 | 0 | 0.0019 | 0.0063 | 0.0224 | 0.0001 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0027 | 0.0003 | 0.0019 | 0.0029 | 0.0045 | 0.0006 | 0.0008 | 0 | 0.0004 | 0 | 0.0012 | 0.0001 | 0.0014 | 0.0001 | 0.0006 | 0.0016 | 0.0019 | 0.0003 |
Endosome | 0.0168 | 0.0004 | 0.0087 | 0.0357 | 0.0277 | 0.0066 | 0.0082 | 0 | 0.001 | 0 | 0.0141 | 0.001 | 0.017 | 0.0006 | 0.009 | 0.0187 | 0.0243 | 0.0022 |
Golgi | 0.0075 | 0 | 0.0039 | 0.0091 | 0.02 | 0.0016 | 0.0021 | 0 | 0.0006 | 0 | 0.0011 | 0.0001 | 0.0141 | 0.0002 | 0.0049 | 0.0152 | 0.006 | 0.0031 |
Lipid Particles | 0.0191 | 0 | 0.0125 | 0.0222 | 0.0918 | 0.0067 | 0.0069 | 0 | 0.0006 | 0 | 0.0023 | 0.0001 | 0.0076 | 0.0002 | 0.0012 | 0.0056 | 0.0277 | 0.0006 |
Mitochondria | 0.024 | 0.0005 | 0.0125 | 0.0736 | 0.0164 | 0.0108 | 0.0012 | 0.0002 | 0.0009 | 0.0001 | 0.0054 | 0.0013 | 0.0306 | 0.0106 | 0.0147 | 0.0138 | 0.0061 | 0.0224 |
None | 0.013 | 0.0017 | 0.0116 | 0.0021 | 0.0043 | 0.0006 | 0.0044 | 0 | 0.0003 | 0 | 0.0014 | 0.0003 | 0.001 | 0.0007 | 0.0002 | 0.0006 | 0.0032 | 0.0001 |
Nuclear Periphery | 0.0139 | 0.0055 | 0.0074 | 0.0157 | 0.0584 | 0.0072 | 0.0105 | 0.0065 | 0.006 | 0.0007 | 0.0406 | 0.0031 | 0.0079 | 0.0069 | 0.0051 | 0.012 | 0.0144 | 0.0016 |
Nucleolus | 0.0512 | 0.0728 | 0.122 | 0.0699 | 0.0267 | 0.1192 | 0.0527 | 0.0544 | 0.1005 | 0.0361 | 0.0372 | 0.1199 | 0.0347 | 0.039 | 0.091 | 0.1395 | 0.0205 | 0.1154 |
Nucleus | 0.7593 | 0.9071 | 0.7776 | 0.6937 | 0.6609 | 0.8318 | 0.8731 | 0.9348 | 0.8789 | 0.9301 | 0.8327 | 0.8695 | 0.7754 | 0.9353 | 0.8437 | 0.6897 | 0.7439 | 0.848 |
Peroxisomes | 0.0165 | 0 | 0.0058 | 0.0109 | 0.0265 | 0.0006 | 0.0033 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0097 | 0.0001 | 0.0014 | 0.0032 | 0.0173 | 0.0003 |
Punctate Nuclear | 0.0166 | 0.005 | 0.004 | 0.0036 | 0.0075 | 0.0045 | 0.0106 | 0.0033 | 0.0024 | 0.0327 | 0.0431 | 0.0015 | 0.0013 | 0.0008 | 0.0019 | 0.0013 | 0.0105 | 0.0006 |
Vacuole | 0.0033 | 0.0003 | 0.0015 | 0.0075 | 0.0102 | 0.0019 | 0.0009 | 0.0001 | 0.0008 | 0 | 0.0054 | 0.0006 | 0.0055 | 0.0016 | 0.0021 | 0.0127 | 0.0038 | 0.0005 |
Vacuole Periphery | 0.0019 | 0.0001 | 0.0011 | 0.0071 | 0.0109 | 0.0011 | 0.0006 | 0.0002 | 0.0004 | 0 | 0.0024 | 0.0005 | 0.0033 | 0.0013 | 0.0016 | 0.0039 | 0.0024 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.9957 | 7.6242 | 16.2652 | 10.6315 | 11.0579 | 9.5895 | 15.8994 | 10.4058 | 11.0332 | 13.7729 |
Translational Efficiency | 2.6283 | 2.5856 | 0.9898 | 1.1932 | 1.6585 | 2.7033 | 1.4672 | 1.8302 | 1.6504 | 1.4297 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1545 | 226 | 1891 | 1556 | 1474 | 1481 | 312 | 1395 | 3019 | 1707 | 2203 | 2951 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 761.92 | 961.83 | 934.49 | 995.45 | 737.05 | 864.08 | 1089.94 | 951.76 | 749.78 | 877.02 | 956.51 | 974.80 |
Standard Deviation | 110.24 | 140.81 | 129.45 | 156.14 | 113.20 | 123.07 | 133.44 | 154.81 | 112.38 | 129.86 | 140.87 | 157.03 |
Intensity Change Log 2 | — | 0.336142 | 0.294540 | 0.385709 | — | 0.229402 | 0.564414 | 0.368835 | — | 0.284644 | 0.433544 | 0.377437 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000227 | 0.001048 | 0.000460 | 0.000924 | 0.000206 | 0.000410 | 0.001048 | 0.000952 | 0.000217 | 0.000494 | 0.000543 | 0.000937 |
Bud Neck | 0.002434 | 0.011136 | 0.020543 | 0.032408 | 0.004845 | 0.006631 | 0.011745 | 0.034305 | 0.003611 | 0.007227 | 0.019297 | 0.033305 |
Bud Site | 0.000710 | 0.004379 | 0.001443 | 0.006365 | 0.001251 | 0.001347 | 0.000960 | 0.009161 | 0.000974 | 0.001749 | 0.001374 | 0.007687 |
Cell Periphery | 0.000106 | 0.000096 | 0.000077 | 0.000098 | 0.000156 | 0.000124 | 0.000050 | 0.000210 | 0.000131 | 0.000121 | 0.000073 | 0.000151 |
Cytoplasm | 0.013599 | 0.003626 | 0.002987 | 0.007225 | 0.009487 | 0.004103 | 0.001373 | 0.026965 | 0.011591 | 0.004040 | 0.002759 | 0.016556 |
Cytoplasmic Foci | 0.001855 | 0.002564 | 0.000026 | 0.001319 | 0.001253 | 0.002167 | 0.000073 | 0.004749 | 0.001561 | 0.002220 | 0.000033 | 0.002940 |
Eisosomes | 0.000017 | 0.000038 | 0.000024 | 0.000028 | 0.000016 | 0.000023 | 0.000038 | 0.000040 | 0.000017 | 0.000025 | 0.000026 | 0.000034 |
Endoplasmic Reticulum | 0.004098 | 0.006483 | 0.004913 | 0.006780 | 0.004810 | 0.006856 | 0.007829 | 0.006137 | 0.004446 | 0.006806 | 0.005326 | 0.006476 |
Endosome | 0.000903 | 0.000799 | 0.000198 | 0.001964 | 0.000621 | 0.001333 | 0.000285 | 0.004766 | 0.000765 | 0.001263 | 0.000210 | 0.003289 |
Golgi | 0.000143 | 0.001025 | 0.000039 | 0.004323 | 0.000183 | 0.001005 | 0.000069 | 0.003510 | 0.000162 | 0.001007 | 0.000043 | 0.003939 |
Lipid Particles | 0.000496 | 0.000303 | 0.000050 | 0.000207 | 0.000596 | 0.001610 | 0.000185 | 0.000715 | 0.000545 | 0.001437 | 0.000069 | 0.000447 |
Mitochondria | 0.001376 | 0.002250 | 0.001436 | 0.003877 | 0.000876 | 0.003601 | 0.001045 | 0.004889 | 0.001132 | 0.003422 | 0.001381 | 0.004356 |
Mitotic Spindle | 0.001234 | 0.001087 | 0.000727 | 0.009047 | 0.001217 | 0.002595 | 0.000202 | 0.022207 | 0.001226 | 0.002396 | 0.000652 | 0.015268 |
None | 0.007548 | 0.002253 | 0.001711 | 0.002666 | 0.006647 | 0.002367 | 0.001557 | 0.009648 | 0.007108 | 0.002352 | 0.001689 | 0.005967 |
Nuclear Periphery | 0.000818 | 0.000417 | 0.000980 | 0.002412 | 0.000966 | 0.001135 | 0.000471 | 0.002090 | 0.000891 | 0.001040 | 0.000908 | 0.002260 |
Nuclear Periphery Foci | 0.000450 | 0.000468 | 0.000243 | 0.000944 | 0.000685 | 0.001022 | 0.000201 | 0.001004 | 0.000564 | 0.000949 | 0.000237 | 0.000972 |
Nucleolus | 0.022963 | 0.027669 | 0.006618 | 0.023236 | 0.019594 | 0.019783 | 0.015877 | 0.027855 | 0.021318 | 0.020827 | 0.007929 | 0.025420 |
Nucleus | 0.938110* | 0.931030* | 0.956037* | 0.885457* | 0.944632* | 0.938946* | 0.953516* | 0.823695* | 0.941294* | 0.937898* | 0.955680* | 0.856261* |
Peroxisomes | 0.000455 | 0.001481 | 0.000094 | 0.000679 | 0.000236 | 0.000989 | 0.000395 | 0.001467 | 0.000348 | 0.001054 | 0.000137 | 0.001052 |
Vacuole | 0.002148 | 0.001559 | 0.001253 | 0.007487 | 0.001445 | 0.002955 | 0.002950 | 0.011274 | 0.001805 | 0.002771 | 0.001493 | 0.009277 |
Vacuole Periphery | 0.000309 | 0.000288 | 0.000141 | 0.002552 | 0.000280 | 0.000998 | 0.000132 | 0.004362 | 0.000295 | 0.000904 | 0.000139 | 0.003408 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.30 | -7.72 | -6.11 | 0.06 | -4.04 | -2.95 | -13.22 | -15.00 | -8.08 | 0.10 | -3.57 | -14.78 | -11.63 | -5.63 | -6.45 |
Bud Neck | -4.22 | -17.84 | -19.58 | -8.82 | -7.21 | -2.29 | -4.41 | -17.58 | -16.31 | -9.83 | -5.45 | -17.14 | -26.24 | -21.41 | -10.54 |
Bud Site | -2.05 | -2.60 | -4.96 | -0.47 | -4.10 | -0.32 | -0.16 | -5.87 | -6.04 | -6.23 | -2.09 | -1.80 | -7.63 | -6.23 | -6.94 |
Cell Periphery | 0.11 | 2.37 | 0.79 | 0.32 | -1.73 | 0.78 | 4.12 | -0.55 | -1.30 | -6.79 | 0.27 | 4.23 | -0.00 | -0.28 | -5.68 |
Cytoplasm | 4.03 | 6.64 | 2.30 | -2.39 | -5.35 | 3.04 | 5.13 | -6.18 | -8.42 | -9.71 | 5.34 | 8.26 | -3.46 | -8.31 | -10.92 |
Cytoplasmic Foci | -0.46 | 4.01 | 2.12 | 1.35 | -3.22 | -1.06 | 3.18 | -1.99 | -0.79 | -4.41 | -0.71 | 5.25 | -0.05 | 0.68 | -5.50 |
Eisosomes | -2.38 | -4.78 | -7.88 | 1.04 | -3.92 | -3.71 | -8.44 | -14.10 | -9.80 | -1.12 | -4.16 | -8.79 | -15.84 | -6.18 | -7.62 |
Endoplasmic Reticulum | -3.58 | -3.28 | -6.91 | -0.52 | -4.72 | -3.59 | -5.54 | -5.30 | -0.36 | 1.98 | -4.98 | -4.33 | -8.61 | -0.31 | -4.71 |
Endosome | 0.11 | 2.59 | -1.53 | -1.49 | -4.39 | -2.42 | 0.95 | -3.95 | -2.54 | -4.21 | -1.85 | 3.39 | -4.14 | -2.29 | -6.03 |
Golgi | -0.96 | 2.77 | -2.93 | -1.48 | -3.06 | -2.20 | 0.97 | -3.09 | -1.47 | -3.30 | -2.47 | 2.24 | -4.08 | -2.23 | -4.32 |
Lipid Particles | 2.02 | 6.60 | 3.27 | 0.80 | -2.81 | -1.47 | 1.36 | -0.06 | 1.49 | -2.02 | -1.54 | 3.92 | 1.03 | 1.82 | -3.97 |
Mitochondria | -0.74 | -0.32 | -3.63 | -1.47 | -4.33 | -2.73 | -1.48 | -6.57 | -0.40 | -5.60 | -2.52 | -1.11 | -6.70 | -0.55 | -6.12 |
Mitotic Spindle | 0.14 | 0.77 | -4.91 | -4.98 | -5.39 | -1.68 | 1.64 | -7.24 | -6.38 | -7.70 | -1.63 | 1.11 | -8.71 | -7.30 | -9.20 |
None | 4.44 | 6.45 | 4.75 | -0.67 | -2.44 | 3.79 | 4.70 | -0.69 | -4.48 | -5.35 | 6.11 | 7.72 | 2.22 | -4.06 | -5.77 |
Nuclear Periphery | 2.70 | -0.94 | -4.94 | -6.28 | -3.69 | -0.92 | 1.85 | -6.26 | -4.51 | -7.19 | -0.97 | -0.57 | -7.47 | -5.57 | -5.25 |
Nuclear Periphery Foci | -0.01 | 1.11 | -1.24 | -0.83 | -1.86 | -0.98 | 1.17 | -1.34 | -0.29 | -2.92 | -1.41 | 1.53 | -1.79 | 0.05 | -3.14 |
Nucleolus | -0.99 | 8.35 | -0.07 | 0.95 | -8.49 | -0.13 | 1.08 | -4.09 | -4.04 | -4.09 | 0.33 | 9.35 | -2.97 | -3.09 | -11.31 |
Nucleus | 0.96 | -3.31 | 10.15 | 4.99 | 14.08 | 1.36 | -0.34 | 17.24 | 15.66 | 16.04 | 1.04 | -3.04 | 19.44 | 16.12 | 22.72 |
Peroxisomes | -1.39 | 3.22 | 0.17 | 1.42 | -2.01 | -2.54 | -2.85 | -3.01 | -0.09 | -2.44 | -2.32 | 3.43 | -1.95 | 0.93 | -3.73 |
Vacuole | -0.05 | 0.34 | -6.12 | -5.93 | -6.93 | -1.85 | -2.08 | -8.30 | -7.53 | -5.16 | -1.61 | -0.86 | -10.31 | -9.16 | -10.07 |
Vacuole Periphery | 0.07 | 2.45 | -3.37 | -3.32 | -3.77 | -2.54 | 1.61 | -3.35 | -2.15 | -3.57 | -2.54 | 2.79 | -4.69 | -2.73 | -5.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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