Standard name
Human Ortholog
Description Alpha-1,6-mannosyltransferase; involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0.05 0.1 0.09 0.09 – 0.08 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.24 0.11 0.1 0.08 0 0 0 – 0 0 0 0.1 0.07 0.16 0.21 0.19 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.3 0.19 0.41 0.61 0.52 0.12 0.14 0.12 – 0.1 0 0.06 0.36 0.47 0.53 0.4 0.44 0.39 0.34 0.32 0.39 0.29 0.29 0.28
Golgi 0.22 0.16 0.05 0 0 0 0 0 – 0 0 0 0.18 0.1 0.11 0.06 0.07 0 0.42 0.35 0.4 0.39 0.39 0.43
Mitochondria 0.37 0.29 0.48 0.32 0.43 0.82 0.75 0.69 – 0.79 0.9 0.79 0.15 0.18 0.08 0.15 0.09 0.1 0.07 0.11 0.07 0.11 0.1 0.08
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.12 0.19 0.09 0.17 0.23 – 0.11 0.09 0.17 0 0.16 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.12 0.12 0 0.09 0.09 – 0.09 0.05 0.08 0.08 0.09 0.12 0.13 0.08 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.23 0.14 0.09 0.07 0 0 0 – 0 0 0 0.17 0.23 0.14 0.33 0.33 0.39 0.13 0.17 0.09 0.11 0.14 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 8 9 0 2 26 4 5 – 6 6 8 38 1 5 4 5 1 3 3 2 13 6 7
Bud 3 7 14 19 25 74 82 85 – 75 55 61 6 2 5 5 5 0 1 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 7 4 3 – 5 14 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 3 2 3 0 2 – 8 4 14 1 1 1 0 0 0 0 0 0 0 1 0
Cytoplasm 42 59 58 34 40 31 19 23 – 24 9 25 31 18 44 55 38 54 0 4 0 1 0 1
Endoplasmic Reticulum 7 19 11 0 0 8 4 1 – 5 1 1 19 3 8 12 4 12 2 1 3 5 2 6
Endosome 130 46 223 216 247 88 123 115 – 98 22 51 114 120 144 108 88 79 173 103 232 146 111 125
Golgi 92 39 26 8 8 15 7 3 – 26 5 6 56 26 31 16 13 9 213 115 238 193 147 191
Mitochondria 160 71 259 113 204 600 650 650 – 734 627 653 48 46 21 39 18 20 34 36 42 52 38 35
Nucleus 0 1 0 0 3 0 2 4 – 2 1 3 0 0 0 2 0 0 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 0 1 1 1 – 6 1 2 0 1 0 1 1 0 1 0 2 0 1 0
Nucleolus 8 0 1 5 7 8 32 92 – 34 26 35 0 0 0 1 3 0 0 0 0 1 0 0
Peroxisomes 2 0 16 42 92 68 147 215 – 105 63 136 10 40 6 6 6 1 11 2 16 19 10 12
SpindlePole 17 0 16 42 56 29 75 88 – 81 32 66 24 22 33 35 15 22 2 0 1 1 0 1
Vac/Vac Membrane 136 56 77 31 31 9 16 23 – 27 8 13 54 58 37 88 65 79 67 55 53 55 53 56
Unique Cell Count 427 248 543 352 473 736 867 942 935 699 823 315 257 274 267 199 204 514 328 597 499 380 448
Labelled Cell Count 611 307 710 513 719 967 1166 1310 1236 874 1079 401 338 335 372 261 277 514 328 597 499 380 448


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 6.7 6.0 6.1 6.4 6.0 5.7 5.9 – 6.2 6.2 6.6 7.3 7.1 6.9 7.9 7.5 7.5 8.7 8.6 8.6
Std Deviation (1e-4) 1.1 1.0 1.2 1.3 1.5 1.1 1.1 1.2 – 1.2 1.2 1.6 1.4 1.1 1.1 1.4 1.5 1.4 1.6 2.3 1.3
Intensity Change (Log2) – – – 0.02 0.09 -0.01 -0.08 -0.02 – 0.05 0.04 0.13 0.29 0.24 0.2 0.39 0.32 0.32 0.54 0.52 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.9 2.0 -2.3 -2.2 – -1.9 -1.3 -1.1 6.5 0 0 0 0 0
Bud 2.2 2.2 5.2 5.0 4.8 – 4.2 4.0 3.8 -0.6 -1.7 -0.7 -0.6 -0.1 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 3.3 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 3.1 0 0 0 0 0 0
Cytoplasm -0.5 -1.2 -4.5 -6.8 -6.7 – -6.6 -7.3 -5.8 -0.4 -1.7 2.2 3.8 3.0 5.4
Endoplasmic Reticulum 0 -3.1 -1.4 -2.8 0 – -2.7 -3.4 0 3.1 0 0.8 2.0 0 2.7
Endosome 5.9 3.6 -12.0 -11.4 -12.8 – -13.7 -16.7 -15.8 -1.4 1.5 3.1 -0.2 0.8 -0.6
Golgi -1.9 -2.7 -2.8 -4.8 -6.0 – -2.0 -4.6 -4.9 6.2 2.9 3.5 0.7 0.9 -0.2
Mitochondria -4.6 -1.5 12.7 10.4 8.1 – 12.2 16.2 12.2 -9.6 -8.1 -11.4 -9.2 -9.6 -9.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 4.2 7.3 – 4.2 4.2 4.6 0 0 0 0 0 0
Peroxisomes 5.3 8.5 4.5 8.0 10.2 – 5.6 4.3 7.8 0.2 6.5 -0.6 -0.6 0 0
SpindlePole 5.3 5.5 1.0 4.2 4.7 – 4.3 1.5 3.9 3.1 3.5 5.2 5.6 2.8 4.3
Vacuole -2.4 -3.9 -9.1 -9.1 -8.7 – -8.2 -9.0 -9.2 1.2 3.0 -0.3 6.2 5.7 7.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0051 0.0127 0.0112 0.0037 0.0074 0.0085 – – – – – – – – – – – –
Bud 0.0015 0.0005 0.0028 0.0002 0.0008 0.0001 – – – – – – – – – – – –
Bud Neck 0.0005 0.0004 0.0003 0.0003 0.0003 0.0003 – – – – – – – – – – – –
Bud Periphery 0.0007 0.0007 0.0007 0.0001 0.002 0.0002 – – – – – – – – – – – –
Bud Site 0.0031 0.0079 0.0038 0.0002 0.0005 0.0002 – – – – – – – – – – – –
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0 – – – – – – – – – – – –
Cytoplasm 0.0038 0.005 0.0083 0.0013 0.0038 0.003 – – – – – – – – – – – –
Cytoplasmic Foci 0.1672 0.1797 0.1581 0.1813 0.158 0.1502 – – – – – – – – – – – –
Eisosomes 0.0001 0.0001 0.0001 0 0.0001 0.0001 – – – – – – – – – – – –
Endoplasmic Reticulum 0.0005 0.0004 0.0006 0.0004 0.0008 0.0005 – – – – – – – – – – – –
Endosome 0.2661 0.2622 0.2633 0.3805 0.3786 0.2049 – – – – – – – – – – – –
Golgi 0.304 0.3732 0.3874 0.2213 0.2805 0.4588 – – – – – – – – – – – –
Lipid Particles 0.0892 0.026 0.0279 0.0275 0.054 0.0418 – – – – – – – – – – – –
Mitochondria 0.0403 0.019 0.0541 0.0274 0.0244 0.0357 – – – – – – – – – – – –
None 0.0206 0.0312 0.0115 0.0003 0.0075 0.0175 – – – – – – – – – – – –
Nuclear Periphery 0.0006 0.0001 0.0012 0.0004 0.0014 0.0005 – – – – – – – – – – – –
Nucleolus 0.0082 0.0001 0.0005 0.0001 0.0003 0.0001 – – – – – – – – – – – –
Nucleus 0.0018 0.0001 0.0022 0.0001 0.0005 0.0002 – – – – – – – – – – – –
Peroxisomes 0.0781 0.0748 0.0589 0.1336 0.0712 0.0736 – – – – – – – – – – – –
Punctate Nuclear 0.005 0.0004 0.0024 0.0003 0.0024 0.0008 – – – – – – – – – – – –
Vacuole 0.0018 0.0029 0.0022 0.0085 0.0027 0.0014 – – – – – – – – – – – –
Vacuole Periphery 0.0015 0.0025 0.0022 0.0124 0.0028 0.0016 – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 82.4542 92.7575 61.4554 71.0398 73.8729 71.2973 69.0842 67.2561 64.4614 80.6301
Translational Efficiency 0.9541 0.8454 1.1653 0.8095 0.8832 0.9863 0.9556 0.8085 0.9936 0.9108

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2050 1215 120 1501 1853 1255 2743 1203 3903 2470 2863 2704

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.81 709.89 958.75 896.43 794.39 807.61 991.40 868.05 746.29 759.54 990.03 883.80
Standard Deviation 88.13 117.50 119.37 159.02 106.41 115.86 131.36 137.36 107.45 126.49 131.04 150.43
Intensity Change Log 2 — 0.014461 0.448020 0.351056 — 0.023811 0.319620 0.127931 — 0.019430 0.381318 0.236976

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002886 0.003029 0.002317 0.001308 0.001617 0.002433 0.001113 0.021563 0.002284 0.002726 0.001164 0.010320
Bud Neck 0.001543 0.001450 0.003441 0.007628 0.000171 0.001081 0.000498 0.008134 0.000892 0.001263 0.000622 0.007853
Bud Site 0.002381 0.001926 0.004927 0.035364 0.000575 0.003621 0.002614 0.045117 0.001524 0.002787 0.002711 0.039703
Cell Periphery 0.000052 0.000351 0.000090 0.000142 0.000051 0.000217 0.000020 0.000120 0.000051 0.000283 0.000022 0.000132
Cytoplasm 0.005438 0.002361 0.005188 0.058384 0.000747 0.002860 0.002990 0.082441 0.003211 0.002614 0.003082 0.069087
Cytoplasmic Foci 0.203294* 0.236150* 0.309004* 0.193062* 0.204358* 0.193824* 0.260095* 0.265681* 0.203799* 0.214645* 0.262145* 0.225370*
Eisosomes 0.000117 0.000062 0.000100 0.000027 0.000045 0.000042 0.000013 0.000105 0.000083 0.000052 0.000017 0.000061
Endoplasmic Reticulum 0.000222 0.000388 0.003247 0.000973 0.000090 0.000189 0.000180 0.000710 0.000160 0.000287 0.000309 0.000856
Endosome 0.158647 0.286432* 0.283671* 0.298808* 0.215140* 0.350685* 0.287001* 0.217695* 0.185467 0.319079* 0.286862* 0.262721*
Golgi 0.528604* 0.403334* 0.140831* 0.086341 0.538628* 0.363196* 0.368234* 0.154900* 0.533363* 0.382940* 0.358703* 0.116842*
Lipid Particles 0.019821 0.023666 0.080939 0.005104 0.007621 0.010317 0.002418 0.009466 0.014029 0.016883 0.005709 0.007045
Mitochondria 0.040169 0.011775 0.006360 0.008079 0.013606 0.025070 0.010168 0.006843 0.027558 0.018530 0.010009 0.007529
Mitotic Spindle 0.001824 0.002083 0.060122 0.084397 0.001477 0.002766 0.025495 0.028399 0.001659 0.002430 0.026946 0.059484
None 0.000215 0.000120 0.000093 0.002054 0.000024 0.000060 0.000026 0.000931 0.000124 0.000089 0.000029 0.001554
Nuclear Periphery 0.000050 0.000021 0.001384 0.000663 0.000007 0.000041 0.000118 0.000147 0.000030 0.000031 0.000171 0.000433
Nuclear Periphery Foci 0.000161 0.000795 0.014129 0.003034 0.000112 0.000692 0.000452 0.000626 0.000138 0.000742 0.001025 0.001962
Nucleolus 0.000119 0.000891 0.003100 0.000712 0.000017 0.000340 0.000047 0.000157 0.000070 0.000611 0.000175 0.000465
Nucleus 0.000382 0.000569 0.009337 0.005386 0.000012 0.000577 0.000434 0.008380 0.000206 0.000573 0.000807 0.006718
Peroxisomes 0.025170 0.018788 0.032600 0.015023 0.011219 0.018317 0.005908 0.057824 0.018547 0.018549 0.007027 0.034065
Vacuole 0.005409 0.003518 0.035037 0.180830 0.002134 0.014349 0.024654 0.086192 0.003854 0.009021 0.025090 0.138726
Vacuole Periphery 0.003494 0.002290 0.004084 0.012682 0.002349 0.009323 0.007522 0.004572 0.002951 0.005863 0.007378 0.009074

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.13 0.69 2.35 2.00 1.97 -1.34 1.29 -7.39 -6.90 -7.59 -0.70 2.44 -6.10 -5.45 -7.29
Bud Neck 0.17 -1.43 -8.38 -7.27 -3.37 -3.01 -3.91 -8.31 -7.11 -7.87 -1.11 1.23 -11.88 -10.32 -12.17
Bud Site 0.70 -1.55 -11.71 -11.94 -9.46 -3.19 -4.78 -12.61 -11.24 -11.84 -2.14 -2.61 -17.21 -16.26 -16.37
Cell Periphery -1.45 -3.03 -2.31 1.06 -0.86 -1.07 1.83 -3.12 0.67 -8.66 -1.80 3.28 -3.40 1.24 -5.20
Cytoplasm 3.33 -0.30 -16.58 -17.32 -15.72 -3.53 -5.75 -18.46 -17.99 -17.91 0.97 -0.83 -24.78 -24.87 -24.56
Cytoplasmic Foci -5.87 -5.48 5.04 9.31 6.80 1.93 -11.33 -5.58 -6.68 2.59 -2.79 -13.98 -0.39 1.92 11.21
Eisosomes 4.94 0.13 10.71 4.48 4.24 0.63 7.62 -7.13 -8.07 -13.11 5.00 13.37 4.05 -1.56 -12.52
Endoplasmic Reticulum -1.26 -2.01 -4.59 -3.10 1.47 -2.58 -2.04 -7.05 -6.13 -5.93 -1.87 -1.98 -7.33 -5.52 -4.92
Endosome -18.08 -5.58 -14.47 2.54 0.64 -16.58 -12.94 3.81 17.74 14.26 -24.43 -21.95 -8.64 13.80 9.72
Golgi 14.46 26.32 66.45* 43.70* 4.44 20.75* 25.40 54.03* 26.37 32.47* 24.72* 29.96 85.72* 49.16* 46.02*
Lipid Particles -1.28 -4.02 8.96 7.03 4.96 -1.37 4.38 -1.21 0.49 -7.25 -1.58 6.57 6.25 6.15 -1.51
Mitochondria 10.26 11.36 13.60 2.49 -0.54 -3.92 2.05 4.81 7.03 3.55 4.38 10.77 13.97 7.19 3.22
Mitotic Spindle -0.48 -3.94 -16.52 -16.45 -1.86 -1.80 -11.60 -9.44 -8.96 -1.04 -1.74 -12.35 -18.70 -18.42 -8.90
None 0.99 0.76 -2.62 -2.77 -2.74 -1.70 -0.36 -2.65 -2.53 -2.63 0.69 2.03 -3.41 -3.50 -3.64
Nuclear Periphery 1.59 -2.68 -3.91 -4.01 0.26 -4.56 -8.57 -11.34 -9.66 -5.66 -0.12 -5.69 -4.66 -4.66 -3.72
Nuclear Periphery Foci -2.36 -3.83 -4.65 -3.58 2.90 -1.49 -4.92 -5.74 -0.61 -3.14 -2.55 -5.14 -5.46 -3.53 -2.95
Nucleolus -1.65 -1.38 -6.87 -0.24 1.13 -1.37 -2.54 -5.08 0.24 -4.15 -2.08 -1.29 -8.13 -0.27 -2.48
Nucleus -0.49 -1.82 -8.13 -7.69 0.07 -1.19 -2.22 -7.96 -7.44 -7.54 -1.20 -2.32 -11.26 -10.55 -9.96
Peroxisomes 2.85 -1.27 5.60 2.20 2.91 -3.20 3.74 -11.66 -8.95 -13.61 -0.04 9.38 -6.71 -6.21 -13.35
Vacuole 2.39 -3.99 -31.19 -31.56 -17.31 -8.30 -15.96 -20.55 -18.26 -16.38 -6.04 -15.18 -36.81 -35.37 -30.58
Vacuole Periphery 2.54 -0.68 -9.89 -10.97 -6.99 -6.74 -10.68 -2.17 4.50 4.62 -5.03 -10.28 -9.18 -2.74 -0.83
Standard name
Human Ortholog
Description Alpha-1,6-mannosyltransferase; involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
Localization
Cell Percentages Golgi (37%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hoc1

Hoc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hoc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available