Standard name
Human Ortholog
Description DNA ligase I found in nucleus and mitochondria; essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination; DNA ligase I mutants trigger ubiquitination of PCNA at K107, facilitating Rad59p-mediated bypass of unligated Okazaki fragments; human homolog LIG1 can complement yeast cdc9 temperature-sensitive mutant at restrictive temperature
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.13 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.07 0 0.16 0.18 0.1 0.26 0.25 0.38 0.25 0 0 0.07 0 0 0.06 0 0 0 0 0.05 0.07
Nucleus 0.87 0.81 0.93 0.88 0.92 0.82 0.87 0.84 0.83 0.81 0.77 0.85 0.92 0.84 0.8 0.7 0.66 0.67 0.68 0.67 0.74 0.46 0.35 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0.06 0.09 0.12 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.21 0.09 0.06 0.05 0.09 0 0 0 0 0 0 0.05 0.06 0.11 0.21 0.16 0.18 0.3 0.28 0.22 0.42 0.52 0.53
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 1 0 0 1 1 1 5 6 2 0 0 0 0 0 0 0 1 0 1 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 3 1 10 5 10 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 2 4 12 6 7 6 11 7 5 7 3 5 11 13 10 11 5 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 1 3 1 0 0 1 1 1 4
Endosome 0 0 0 0 0 1 0 1 0 0 0 0 1 7 5 0 1 0 1 0 3 0 2 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 2 2 2 3 0
Mitochondria 4 10 4 18 9 52 80 49 113 118 127 108 10 11 19 0 1 3 1 2 2 2 12 20
Nucleus 159 179 248 238 281 259 388 398 356 389 259 371 239 224 218 69 57 34 128 165 208 49 87 89
Nuclear Periphery 0 0 0 0 0 1 3 4 4 6 5 2 2 1 2 0 0 0 0 0 0 0 1 3
Nucleolus 0 2 0 3 0 4 13 23 27 42 42 27 0 5 2 1 1 2 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 3 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 2 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 21 46 25 16 16 29 17 18 6 18 7 9 12 17 30 21 14 9 56 69 62 44 129 154
Unique Cell Count 183 222 267 272 305 316 446 475 428 481 338 437 261 266 273 98 87 51 190 246 284 107 248 290
Labelled Cell Count 190 239 282 290 312 355 512 509 524 588 463 539 271 280 292 102 90 55 190 246 284 107 248 290


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 8.9 7.8 7.5 7.9 6.4 6.6 6.9 6.0 5.9 5.3 5.8 8.6 8.8 8.6 14.0 15.8 13.5 7.8 7.4 7.7
Std Deviation (1e-4) 2.2 2.2 1.4 1.6 1.4 1.2 1.4 1.5 1.4 1.3 1.2 1.1 2.3 2.6 2.8 4.0 6.2 3.1 2.0 1.3 1.8
Intensity Change (Log2) – – – -0.05 0.02 -0.28 -0.24 -0.19 -0.37 -0.4 -0.55 -0.43 0.15 0.17 0.14 0.85 1.02 0.79 -0.01 -0.08 -0.03

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 0 0.6 0 0.8 0 0 0 0 0 1.8 2.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 1.2 6.1 6.6 4.5 8.5 8.2 10.7 8.2 1.7 1.8 3.1 0 0 0
Nucleus -2.1 -0.3 -3.9 -2.5 -3.5 -3.7 -4.4 -5.4 -3.2 -0.6 -3.1 -4.4 -5.6 -6.4 -5.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.7 4.2 5.0 6.0 4.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 -1.9 -0.1 -3.0 -3.1 -4.9 -3.2 -4.0 -4.4 -2.1 -1.3 0.6 3.1 1.7 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5754 4.7884 4.7903 3.4952 3.5575 4.3284 3.975 5.9638 5.9134 5.3132 3.9491 5.0874 4.218 5.6392 5.5029 4.9255 4.419 4.7102
Actin 0.0654 0.0001 0.0035 0.0114 0.0459 0.0214 0.0037 0.0001 0.006 0.0106 0.0016 0.0002 0.028 0.0001 0.0066 0.0006 0.0001 0.0002
Bud 0.0009 0.0002 0.0005 0.0022 0.0033 0.0006 0.0004 0.0001 0.0006 0.0002 0.0007 0.0001 0.0004 0.0001 0.0002 0.0005 0.0001 0.0001
Bud Neck 0.0037 0.0002 0.0006 0.0003 0.0033 0.0017 0.0007 0.0002 0.0022 0.0002 0.0014 0.0022 0.0024 0.0001 0.0002 0.0003 0.0002 0.0032
Bud Periphery 0.0022 0.0004 0.0011 0.0013 0.005 0.0012 0.0008 0.0006 0.0009 0.0005 0.0054 0.0004 0.0007 0.0001 0.0003 0.0014 0.0002 0.0004
Bud Site 0.0028 0.0006 0.0106 0.0049 0.049 0.0012 0.0006 0.0001 0.0038 0.0005 0.0006 0.0001 0.0042 0.0001 0.0003 0.0004 0.0001 0.0001
Cell Periphery 0.0006 0.0002 0.0011 0.0001 0.0011 0.0002 0.0002 0.0003 0.0004 0.0002 0.0012 0.0002 0.0002 0.0001 0.0001 0.0004 0 0.0002
Cytoplasm 0.0072 0.0003 0.0021 0.0062 0.0099 0.0006 0.0022 0.0003 0.0009 0.0006 0.0006 0.0003 0.0026 0.0004 0.0002 0.001 0.0002 0.0002
Cytoplasmic Foci 0.0333 0.0002 0.004 0.0224 0.0342 0.0035 0.02 0 0.0067 0.0095 0.0001 0 0.0175 0 0.0018 0.0002 0 0.0002
Eisosomes 0.001 0 0.0002 0 0.0004 0.0001 0 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0089 0 0.0013 0.0004 0.0119 0.0039 0.0043 0.0002 0.0004 0.001 0.0024 0.0003 0.0046 0.0001 0.0001 0.0004 0.0001 0.0001
Endosome 0.0495 0.0004 0.0058 0.0181 0.0713 0.0114 0.0317 0.0002 0.0061 0.003 0.008 0.0002 0.0206 0 0.0017 0.0019 0.0001 0.0007
Golgi 0.0167 0.0001 0.0016 0.0122 0.0112 0.0091 0.0108 0 0.0074 0.0044 0.0006 0 0.0087 0 0.0036 0.0005 0 0.0004
Lipid Particles 0.0401 0.0007 0.009 0.0012 0.0143 0.0058 0.0249 0 0.0103 0.021 0.0009 0 0.0151 0 0.0013 0.0003 0 0.0026
Mitochondria 0.0199 0.0086 0.013 0.0041 0.0452 0.0091 0.0072 0.0042 0.0152 0.0209 0.0369 0.0015 0.0061 0.0017 0.0144 0.059 0.0033 0.007
None 0.0095 0 0.0024 0.0003 0.0029 0.0003 0.0006 0.0001 0.0004 0.0005 0.0005 0 0.0024 0 0 0.0001 0.0001 0
Nuclear Periphery 0.0284 0.0052 0.0087 0.0014 0.0431 0.0148 0.0492 0.012 0.0076 0.0205 0.0652 0.0067 0.0175 0.0106 0.0047 0.01 0.0071 0.0026
Nucleolus 0.0144 0.013 0.0079 0.0074 0.0421 0.0184 0.0121 0.0146 0.0066 0.0058 0.0429 0.0189 0.0097 0.0103 0.0073 0.0104 0.0156 0.0253
Nucleus 0.6545 0.9657 0.9153 0.8992 0.5552 0.8887 0.8023 0.9587 0.9125 0.8755 0.7927 0.9664 0.8199 0.9752 0.9527 0.9026 0.9708 0.9533
Peroxisomes 0.01 0.0004 0.0048 0.0051 0.0048 0.0018 0.0147 0 0.0072 0.013 0.0002 0 0.0245 0 0.0018 0.0003 0 0.0003
Punctate Nuclear 0.0211 0.0002 0.0016 0.0007 0.006 0.0031 0.0047 0.0005 0.0028 0.008 0.001 0.0003 0.0024 0.0003 0.0001 0.0001 0.0002 0.0002
Vacuole 0.0063 0.002 0.0034 0.0007 0.0219 0.0013 0.0037 0.0035 0.0011 0.0013 0.016 0.0014 0.0093 0.0004 0.001 0.005 0.0005 0.0016
Vacuole Periphery 0.0033 0.0016 0.0015 0.0004 0.018 0.0015 0.0054 0.0043 0.0009 0.0027 0.0209 0.0008 0.0031 0.0005 0.0015 0.0047 0.0014 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.5696 10.0029 10.3271 24.3676 36.2699 37.9268 12.9722 12.9105 19.5978 39.5739
Translational Efficiency 1.678 1.4698 1.1595 1.2546 1.2432 1.8195 0.8955 0.7498 0.9503 1.0003

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1866 706 1318 1154 735 1855 1553 445 2601 2561 2871 1599

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 760.86 934.24 1263.74 1263.32 851.96 976.96 1189.96 1356.01 786.60 965.18 1223.83 1289.12
Standard Deviation 108.20 173.08 200.39 217.50 124.33 148.80 174.82 250.31 120.20 157.03 190.57 230.88
Intensity Change Log 2 — 0.296162 0.731997 0.731517 — 0.197514 0.482055 0.670510 — 0.244893 0.605373 0.699612

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000981 0.004662 0.002356 0.012317 0.003449 0.002933 0.002839 0.019465 0.001678 0.003410 0.002618 0.014306
Bud Neck 0.016563 0.039604 0.046055 0.197914 0.028500 0.039735 0.057138 0.185816 0.019937 0.039699 0.052050 0.194547
Bud Site 0.006271 0.012455 0.003426 0.053441 0.009529 0.009001 0.006378 0.055315 0.007191 0.009953 0.005023 0.053962
Cell Periphery 0.000627 0.000579 0.000201 0.000745 0.000638 0.000544 0.000286 0.000774 0.000630 0.000553 0.000247 0.000753
Cytoplasm 0.015488 0.003713 0.001071 0.006434 0.002135 0.001828 0.001688 0.004042 0.011715 0.002347 0.001405 0.005768
Cytoplasmic Foci 0.005945 0.001360 0.000075 0.004394 0.002115 0.001348 0.000393 0.001008 0.004863 0.001351 0.000247 0.003452
Eisosomes 0.000049 0.000120 0.000060 0.000145 0.000097 0.000105 0.000071 0.000203 0.000063 0.000109 0.000066 0.000161
Endoplasmic Reticulum 0.021098 0.036982 0.008479 0.030416 0.041705 0.060467 0.022434 0.034796 0.026921 0.053993 0.016028 0.031635
Endosome 0.001641 0.001871 0.000312 0.006267 0.001697 0.002207 0.000722 0.001541 0.001657 0.002115 0.000534 0.004952
Golgi 0.001420 0.001297 0.000087 0.008653 0.000724 0.001349 0.000124 0.000396 0.001224 0.001335 0.000107 0.006355
Lipid Particles 0.003201 0.001683 0.000128 0.001768 0.002930 0.001756 0.000330 0.001551 0.003124 0.001736 0.000237 0.001707
Mitochondria 0.019111 0.032647 0.005697 0.057977 0.031298 0.026481 0.009007 0.042524 0.022555 0.028181 0.007488 0.053676
Mitotic Spindle 0.004102 0.008022 0.001242 0.049996 0.008149 0.004615 0.004145 0.028967 0.005246 0.005554 0.002812 0.044144
None 0.013566 0.005042 0.000890 0.002374 0.003692 0.002771 0.001613 0.002801 0.010775 0.003397 0.001281 0.002493
Nuclear Periphery 0.003431 0.002077 0.001358 0.007024 0.004486 0.005199 0.002984 0.003579 0.003729 0.004338 0.002237 0.006066
Nuclear Periphery Foci 0.001264 0.000793 0.000123 0.002843 0.001713 0.001472 0.000958 0.002118 0.001391 0.001285 0.000575 0.002641
Nucleolus 0.017705 0.021815 0.007045 0.011367 0.027846 0.020392 0.008435 0.010815 0.020571 0.020785 0.007797 0.011213
Nucleus 0.853687 0.803532 0.917399 0.505934 0.810098 0.796649 0.874174 0.576740 0.841369 0.798547 0.894018 0.525639
Peroxisomes 0.005769 0.007103 0.000714 0.003809 0.009421 0.005065 0.001483 0.006306 0.006801 0.005627 0.001130 0.004504
Vacuole 0.005667 0.010803 0.002744 0.029288 0.006844 0.012833 0.004280 0.018207 0.006000 0.012274 0.003575 0.026204
Vacuole Periphery 0.002414 0.003840 0.000538 0.006895 0.002933 0.003249 0.000517 0.003036 0.002561 0.003412 0.000527 0.005821

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.81 -2.80 -11.84 -7.50 -8.97 1.72 1.09 -7.34 -7.66 -7.56 -9.46 -4.05 -13.70 -11.76 -12.04
Bud Neck -7.24 -12.65 -32.24 -25.67 -25.37 -3.48 -9.15 -18.82 -17.86 -15.00 -10.00 -17.20 -37.30 -32.40 -28.91
Bud Site -4.27 3.08 -18.69 -14.63 -20.09 0.51 1.90 -10.81 -11.22 -11.64 -3.47 2.19 -21.53 -20.23 -22.66
Cell Periphery 0.48 6.40 -0.99 -1.43 -10.23 0.65 2.54 -0.93 -3.11 -7.06 1.11 6.24 -1.35 -3.44 -11.98
Cytoplasm 6.54 11.47 6.64 -2.12 -11.91 0.48 0.92 -3.14 -3.69 -4.77 9.14 11.20 5.87 -6.19 -12.23
Cytoplasmic Foci 5.30 7.32 2.89 -2.56 -5.08 1.49 3.26 2.16 0.84 -2.18 5.59 7.66 3.26 -2.42 -5.06
Eisosomes -8.48 -3.84 -13.40 -2.29 -10.44 -1.15 4.20 -6.95 -6.65 -8.83 -11.28 -2.21 -14.59 -7.13 -13.54
Endoplasmic Reticulum -7.44 11.37 -5.19 2.57 -12.91 -6.44 7.61 1.63 7.20 -4.14 -15.71 9.27 -3.05 10.87 -10.12
Endosome -0.84 10.82 -4.96 -4.23 -7.03 -1.76 3.95 -0.00 2.09 -5.22 -2.53 9.49 -4.88 -3.77 -7.29
Golgi 0.16 3.48 -2.84 -2.83 -3.81 -1.17 2.89 1.40 1.88 -6.15 -0.28 3.98 -2.76 -2.57 -3.87
Lipid Particles 1.94 6.23 2.33 -0.32 -6.83 1.87 4.58 1.61 -0.60 -7.05 2.92 7.41 2.78 -0.56 -8.42
Mitochondria -3.19 7.05 -10.26 -4.17 -17.46 1.27 6.47 -2.44 -4.82 -12.08 -2.44 8.91 -10.19 -7.99 -20.14
Mitotic Spindle -1.61 2.80 -9.23 -7.55 -9.93 1.74 1.47 -3.49 -4.23 -4.13 -0.23 1.80 -9.79 -9.60 -10.36
None 5.83 11.02 9.89 3.12 -7.35 1.31 3.35 1.77 0.67 -1.78 8.02 11.14 9.98 3.01 -4.68
Nuclear Periphery 2.54 4.37 -4.20 -6.06 -7.15 -0.69 1.37 0.41 1.91 -1.97 -1.15 3.14 -3.66 -3.00 -6.61
Nuclear Periphery Foci 1.67 5.46 -4.48 -5.87 -8.77 0.38 1.21 -1.26 -1.72 -2.25 0.23 2.64 -4.23 -4.50 -6.12
Nucleolus -1.84 8.03 4.62 5.05 -3.72 3.20 8.51 6.80 5.41 -1.48 -0.08 11.08 7.68 8.00 -3.53
Nucleus 4.92 -9.21 32.75 23.11 41.25 1.22 -5.80 14.94 15.53 20.29 6.68 -8.45 35.62 30.83 43.99
Peroxisomes -0.98 5.88 2.56 3.56 -6.13 3.20 6.38 2.44 -0.68 -4.53 1.40 7.90 3.49 2.25 -6.80
Vacuole -4.15 3.43 -13.64 -9.81 -15.17 -5.28 2.09 -7.32 -4.59 -8.35 -7.54 3.12 -15.41 -10.74 -16.90
Vacuole Periphery -1.87 4.59 -2.93 -1.23 -5.42 -0.44 3.48 0.36 1.02 -3.64 -1.97 5.84 -2.61 -1.48 -6.18
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description DNA ligase I found in nucleus and mitochondria; essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination; DNA ligase I mutants trigger ubiquitination of PCNA at K107, facilitating Rad59p-mediated bypass of unligated Okazaki fragments; human homolog LIG1 can complement yeast cdc9 temperature-sensitive mutant at restrictive temperature
Localization
Cell Percentages nucleus (47%), mitochondrion (12%), mixed (24%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc9

Cdc9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available