Standard name | |
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Human Ortholog | |
Description | Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 8 | 2 | 4 | 17 | 19 | 9 | 13 | 20 | 31 | 53 | 37 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 2 | 5 | 3 | 3 | 2 | 8 | 18 | 29 | 3 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 1 | 0 | 6 | 5 | 0 | 6 | 7 | 14 | 18 | 23 | 5 | 3 | 6 | 7 | 10 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 230 | 270 | 87 | 70 | 129 | 48 | 70 | 73 | 46 | 74 | 51 | 54 | 167 | 171 | 164 | 516 | 294 | 239 | 212 | 270 | 83 | 173 | 238 | 204 |
Endoplasmic Reticulum | 2 | 2 | 0 | 1 | 11 | 6 | 17 | 17 | 22 | 23 | 20 | 22 | 7 | 16 | 7 | 11 | 9 | 7 | 13 | 5 | 16 | 13 | 24 | 35 |
Endosome | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 5 | 1 | 0 | 0 | 0 | 2 | 7 | 13 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 4 | 3 | 1 | 0 | 2 | 28 | 60 | 49 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 4 | 4 | 5 | 9 | 9 | 19 | 29 | 15 | 31 | 44 | 31 | 0 | 3 | 4 | 7 | 6 | 2 | 0 | 1 | 2 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 3 | 1 | 0 | 8 | 9 | 2 | 5 | 7 | 3 | 3 | 5 | 4 | 26 | 16 | 7 | 1 | 3 | 2 | 1 | 3 | 5 |
Unique Cell Count | 234 | 277 | 92 | 79 | 155 | 63 | 112 | 126 | 93 | 133 | 120 | 121 | 221 | 278 | 260 | 554 | 329 | 250 | 233 | 288 | 111 | 193 | 273 | 251 |
Labelled Cell Count | 237 | 285 | 95 | 82 | 171 | 73 | 121 | 163 | 121 | 160 | 160 | 160 | 254 | 339 | 321 | 579 | 346 | 261 | 233 | 288 | 111 | 193 | 273 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.1 | 20.1 | 15.1 | 13.2 | 14.5 | 14.1 | 11.6 | 12.4 | 13.9 | 12.8 | 13.0 | 12.9 | 21.0 | 21.5 | 21.2 | 20.1 | 23.5 | 23.9 | 19.0 | 18.7 | 21.5 |
Std Deviation (1e-4) | 3.7 | 3.9 | 3.3 | 3.3 | 3.1 | 3.0 | 3.7 | 3.1 | 3.7 | 3.5 | 3.6 | 3.4 | 3.5 | 3.9 | 4.6 | 5.6 | 6.8 | 6.8 | 3.4 | 3.8 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.06 | -0.1 | -0.38 | -0.29 | -0.13 | -0.24 | -0.22 | -0.24 | 0.47 | 0.51 | 0.49 | 0.41 | 0.63 | 0.66 | 0.32 | 0.31 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.7 | 4.6 | 0 | 3.3 | 4.1 | 3.8 | 4.5 | 3.8 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.5 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.4 | -2.6 | -3.4 | -5.4 | -6.0 | -6.8 | -6.4 | -7.9 | -7.6 | -3.9 | -6.0 | -5.7 | -0.5 | -1.5 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.9 | 3.7 | 5.0 | 4.2 | 4.1 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.9 | 4.5 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.2 | 2.8 | 3.8 | 2.6 | 3.9 | 5.6 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.4409 | 25.0166 | 23.2144 | 20.5867 | 21.5982 | 23.1162 | 14.216 | 17.7092 | 15.7434 | 15.3061 | 16.2711 | 17.3215 | 17.374 | 22.7582 | 21.5385 | 19.9578 | 20.5743 | 21.2963 |
Actin | 0.0494 | 0.0007 | 0.0033 | 0.025 | 0.0004 | 0.0019 | 0.0046 | 0.0094 | 0.0348 | 0.0018 | 0.0012 | 0.0013 | 0.009 | 0.001 | 0.0033 | 0.0014 | 0.0014 | 0.0017 |
Bud | 0.0008 | 0.0004 | 0.0011 | 0.0008 | 0.0002 | 0.0005 | 0.0004 | 0.0015 | 0.0007 | 0.0008 | 0.0005 | 0.0003 | 0.0008 | 0.0062 | 0.0003 | 0.0005 | 0.0002 | 0.0011 |
Bud Neck | 0.0009 | 0.0004 | 0.0011 | 0.0021 | 0.0012 | 0.0029 | 0.0007 | 0.0003 | 0.0007 | 0.0005 | 0.0004 | 0.001 | 0.0013 | 0.0003 | 0.0006 | 0.0007 | 0.001 | 0.0014 |
Bud Periphery | 0.0011 | 0.0003 | 0.001 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0007 | 0.0023 | 0.0001 | 0.0003 | 0.0001 | 0.0003 |
Bud Site | 0.0021 | 0.001 | 0.002 | 0.0012 | 0 | 0.0002 | 0.0019 | 0.0043 | 0.0118 | 0.0002 | 0.001 | 0.0001 | 0.0021 | 0.0038 | 0.0003 | 0.0007 | 0.0002 | 0.0001 |
Cell Periphery | 0.0013 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.5102 | 0.8763 | 0.8218 | 0.6689 | 0.9059 | 0.746 | 0.7128 | 0.9523 | 0.7855 | 0.8369 | 0.8432 | 0.8448 | 0.7 | 0.9447 | 0.9027 | 0.8531 | 0.8619 | 0.8028 |
Cytoplasmic Foci | 0.0274 | 0.0028 | 0.0118 | 0.04 | 0.0043 | 0.0108 | 0.0336 | 0.0059 | 0.0244 | 0.0174 | 0.0422 | 0.0224 | 0.0341 | 0.0036 | 0.0104 | 0.0132 | 0.0126 | 0.015 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.134 | 0.0681 | 0.0536 | 0.0359 | 0.0382 | 0.1459 | 0.1292 | 0.014 | 0.03 | 0.0067 | 0.01 | 0.0448 | 0.0945 | 0.0196 | 0.0257 | 0.0127 | 0.0678 | 0.0922 |
Endosome | 0.0693 | 0.0181 | 0.0292 | 0.0683 | 0.0232 | 0.0482 | 0.053 | 0.0049 | 0.0527 | 0.1028 | 0.0552 | 0.0529 | 0.0715 | 0.0061 | 0.0306 | 0.044 | 0.0246 | 0.0491 |
Golgi | 0.0143 | 0.0024 | 0.0027 | 0.0141 | 0.001 | 0.0044 | 0.0074 | 0.0012 | 0.0173 | 0.0186 | 0.0043 | 0.0082 | 0.0084 | 0.0013 | 0.0107 | 0.0076 | 0.0017 | 0.0085 |
Lipid Particles | 0.0195 | 0.0001 | 0.0021 | 0.0271 | 0 | 0.0002 | 0.0085 | 0.0001 | 0.0022 | 0.0011 | 0.013 | 0.0033 | 0.0083 | 0.0001 | 0.0006 | 0.0128 | 0.0037 | 0.0002 |
Mitochondria | 0.0303 | 0.0031 | 0.0053 | 0.0112 | 0.0006 | 0.0047 | 0.0023 | 0.0004 | 0.0167 | 0.0016 | 0.0087 | 0.0109 | 0.0057 | 0.0034 | 0.0012 | 0.0269 | 0.0009 | 0.005 |
None | 0.0098 | 0.0004 | 0.0019 | 0.0008 | 0.0003 | 0.0005 | 0.0021 | 0.0004 | 0.0024 | 0.0004 | 0.0041 | 0.0015 | 0.0053 | 0.0003 | 0.0004 | 0.0003 | 0.0039 | 0.0071 |
Nuclear Periphery | 0.0483 | 0.011 | 0.0133 | 0.0178 | 0.0085 | 0.013 | 0.0174 | 0.0016 | 0.0051 | 0.0015 | 0.0021 | 0.0021 | 0.0164 | 0.0013 | 0.0039 | 0.005 | 0.0064 | 0.004 |
Nucleolus | 0.0009 | 0.0001 | 0.0006 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0005 |
Nucleus | 0.0088 | 0.0029 | 0.0053 | 0.004 | 0.0033 | 0.0042 | 0.0032 | 0.0009 | 0.0015 | 0.0008 | 0.0013 | 0.001 | 0.0046 | 0.0011 | 0.0012 | 0.002 | 0.0061 | 0.002 |
Peroxisomes | 0.0205 | 0 | 0.0008 | 0.0132 | 0 | 0.0001 | 0.0089 | 0.0004 | 0.0016 | 0.0002 | 0.0015 | 0.0004 | 0.003 | 0.0001 | 0.0002 | 0.0027 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0084 | 0.0001 | 0.0077 | 0.0027 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0018 | 0.0002 | 0.0022 | 0.0003 | 0.0081 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0001 |
Vacuole | 0.0317 | 0.008 | 0.026 | 0.0423 | 0.009 | 0.0106 | 0.0101 | 0.0012 | 0.0056 | 0.0052 | 0.0048 | 0.0026 | 0.0196 | 0.0034 | 0.0057 | 0.0095 | 0.0049 | 0.0054 |
Vacuole Periphery | 0.0101 | 0.0036 | 0.0085 | 0.0232 | 0.0034 | 0.0052 | 0.0026 | 0.0003 | 0.0036 | 0.0032 | 0.0028 | 0.0019 | 0.0046 | 0.0007 | 0.0018 | 0.0058 | 0.0015 | 0.0032 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 95.3962 | 75.8751 | 90.9503 | 116.4351 | 102.5225 | 80.48 | 89.8313 | 102.4515 | 89.863 | 107.7986 |
Translational Efficiency | 1.9706 | 2.0406 | 1.8255 | 1.292 | 1.5775 | 2.5443 | 1.7559 | 1.4827 | 1.8505 | 1.6672 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2096 | 1229 | 2084 | 185 | 1678 | 1914 | 178 | 1247 | 3774 | 3143 | 2262 | 1432 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1513.47 | 1427.43 | 2174.86 | 2164.56 | 1414.36 | 1623.82 | 2228.25 | 2133.06 | 1469.40 | 1547.03 | 2179.06 | 2137.13 |
Standard Deviation | 236.59 | 267.06 | 328.16 | 464.23 | 215.69 | 274.81 | 289.90 | 429.55 | 232.80 | 288.21 | 325.63 | 434.32 |
Intensity Change Log 2 | — | -0.084440 | 0.523062 | 0.516214 | — | 0.199242 | 0.655762 | 0.592775 | — | 0.059569 | 0.588691 | 0.553706 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000225 | 0.000530 | 0.000149 | 0.001660 | 0.000199 | 0.000337 | 0.000878 | 0.001940 | 0.000213 | 0.000412 | 0.000206 | 0.001904 |
Bud Neck | 0.000250 | 0.000472 | 0.001397 | 0.007304 | 0.000199 | 0.000359 | 0.006337 | 0.003859 | 0.000227 | 0.000403 | 0.001786 | 0.004304 |
Bud Site | 0.012002 | 0.019418 | 0.008799 | 0.063249 | 0.012115 | 0.016491 | 0.028522 | 0.038684 | 0.012052 | 0.017636 | 0.010351 | 0.041857 |
Cell Periphery | 0.000585 | 0.001453 | 0.001404 | 0.006622 | 0.001154 | 0.000422 | 0.002152 | 0.001809 | 0.000838 | 0.000825 | 0.001463 | 0.002431 |
Cytoplasm | 0.610653* | 0.608272* | 0.694701* | 0.413064* | 0.643495* | 0.647289* | 0.537165* | 0.557744* | 0.625255* | 0.632032* | 0.682304* | 0.539053* |
Cytoplasmic Foci | 0.198665* | 0.146795 | 0.024310 | 0.182214* | 0.181370* | 0.166401 | 0.070830 | 0.101343 | 0.190975* | 0.158735 | 0.027970 | 0.111791 |
Eisosomes | 0.000003 | 0.000005 | 0.000002 | 0.000014 | 0.000004 | 0.000004 | 0.000006 | 0.000010 | 0.000003 | 0.000004 | 0.000002 | 0.000010 |
Endoplasmic Reticulum | 0.025086 | 0.018863 | 0.005892 | 0.000741 | 0.033860 | 0.013122 | 0.004387 | 0.002652 | 0.028987 | 0.015367 | 0.005774 | 0.002405 |
Endosome | 0.007207 | 0.012990 | 0.006144 | 0.021855 | 0.008772 | 0.011511 | 0.022700 | 0.018681 | 0.007903 | 0.012090 | 0.007447 | 0.019091 |
Golgi | 0.054084 | 0.071808 | 0.027176 | 0.040496 | 0.049105 | 0.056452 | 0.066094 | 0.050402 | 0.051870 | 0.062457 | 0.030238 | 0.049122 |
Lipid Particles | 0.001058 | 0.005806 | 0.006899 | 0.046576 | 0.003403 | 0.001480 | 0.018840 | 0.020532 | 0.002101 | 0.003172 | 0.007839 | 0.023897 |
Mitochondria | 0.001083 | 0.001007 | 0.000208 | 0.000205 | 0.000638 | 0.001005 | 0.000044 | 0.001305 | 0.000885 | 0.001006 | 0.000195 | 0.001163 |
Mitotic Spindle | 0.004448 | 0.003762 | 0.008391 | 0.042046 | 0.002014 | 0.006601 | 0.026017 | 0.051705 | 0.003366 | 0.005491 | 0.009778 | 0.050458 |
None | 0.001177 | 0.000720 | 0.000580 | 0.000443 | 0.000792 | 0.000464 | 0.000217 | 0.000645 | 0.001006 | 0.000564 | 0.000551 | 0.000619 |
Nuclear Periphery | 0.000201 | 0.000183 | 0.000527 | 0.000181 | 0.000120 | 0.000109 | 0.000543 | 0.000394 | 0.000165 | 0.000138 | 0.000528 | 0.000366 |
Nuclear Periphery Foci | 0.005688 | 0.012746 | 0.017607 | 0.006958 | 0.006042 | 0.004547 | 0.032537 | 0.008631 | 0.005845 | 0.007753 | 0.018782 | 0.008415 |
Nucleolus | 0.000031 | 0.000117 | 0.000085 | 0.000200 | 0.000050 | 0.000088 | 0.000061 | 0.000326 | 0.000040 | 0.000099 | 0.000083 | 0.000309 |
Nucleus | 0.001124 | 0.002070 | 0.004297 | 0.005039 | 0.001224 | 0.001435 | 0.004525 | 0.005254 | 0.001168 | 0.001684 | 0.004315 | 0.005226 |
Peroxisomes | 0.000076 | 0.000221 | 0.000054 | 0.004036 | 0.000098 | 0.000135 | 0.000301 | 0.001479 | 0.000086 | 0.000169 | 0.000073 | 0.001809 |
Vacuole | 0.013488 | 0.042591 | 0.148730 | 0.142811 | 0.018034 | 0.029747 | 0.150290 | 0.110021 | 0.015509 | 0.034770 | 0.148853 | 0.114257 |
Vacuole Periphery | 0.062868 | 0.050171 | 0.042649 | 0.014284 | 0.037313 | 0.041999 | 0.027552 | 0.022584 | 0.051506 | 0.045195 | 0.041461 | 0.021512 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.47 | 0.79 | -3.61 | -2.47 | -3.82 | -1.17 | -3.07 | -3.38 | -3.01 | -1.77 | -1.79 | -0.25 | -3.74 | -3.17 | -3.73 |
Bud Neck | -3.51 | -10.68 | -7.70 | -7.52 | -6.58 | -5.69 | -7.07 | -14.84 | -14.25 | 2.40 | -4.87 | -13.14 | -16.37 | -15.84 | -9.70 |
Bud Site | -5.87 | 2.95 | -7.43 | -6.34 | -7.82 | -3.75 | -4.65 | -11.63 | -9.50 | -2.68 | -6.51 | 1.44 | -13.77 | -11.03 | -13.96 |
Cell Periphery | -2.68 | -6.14 | -3.24 | -2.57 | -2.66 | 4.96 | -2.23 | -1.62 | -4.87 | 1.07 | 0.09 | -4.48 | -4.29 | -4.05 | -2.01 |
Cytoplasm | 0.23 | -7.79 | 8.44 | 8.14 | 11.36 | -0.34 | 4.47 | 8.47 | 9.02 | -0.38 | -0.85 | -5.84 | 10.00 | 10.42 | 13.60 |
Cytoplasmic Foci | 7.65 | 36.42 | 0.24 | -2.42 | -8.95 | 2.35 | 14.73 | 10.11 | 8.63 | -5.27 | 6.97 | 45.59 | 11.48 | 6.29 | -17.95 |
Eisosomes | -5.13 | 3.95 | -3.82 | -2.87 | -4.07 | -0.20 | -3.01 | -5.08 | -4.70 | -1.91 | -3.53 | 4.36 | -6.48 | -5.08 | -7.16 |
Endoplasmic Reticulum | 2.48 | 10.53 | 14.09 | 10.05 | 8.72 | 8.34 | 12.26 | 13.55 | 8.35 | 1.62 | 8.04 | 15.66 | 17.91 | 11.64 | 4.56 |
Endosome | -8.96 | 2.14 | -4.07 | -2.25 | -4.34 | -4.29 | -6.42 | -8.03 | -5.40 | 2.18 | -9.35 | 0.57 | -9.92 | -5.61 | -9.95 |
Golgi | -5.33 | 12.95 | 3.75 | 6.38 | -1.04 | -2.97 | -1.69 | 1.66 | 3.90 | 2.28 | -5.33 | 12.65 | 3.45 | 6.90 | -4.59 |
Lipid Particles | -6.28 | -13.26 | -5.68 | -5.06 | -4.90 | 6.00 | -5.26 | -10.70 | -11.95 | -0.44 | -3.26 | -11.89 | -12.61 | -11.87 | -8.93 |
Mitochondria | 0.22 | 6.93 | 5.40 | 2.28 | 0.25 | -1.26 | 4.65 | -1.45 | -0.25 | -3.61 | -0.53 | 7.46 | -0.36 | 0.07 | -3.07 |
Mitotic Spindle | 0.54 | -3.90 | -4.41 | -4.46 | -3.94 | -4.91 | -4.48 | -12.41 | -11.31 | -3.95 | -3.02 | -6.38 | -12.95 | -12.36 | -10.90 |
None | 2.48 | 4.68 | 3.48 | 0.97 | 0.37 | 1.92 | 4.03 | 0.85 | -0.99 | -3.01 | 3.66 | 4.27 | 2.70 | -0.33 | -0.50 |
Nuclear Periphery | 0.49 | -5.57 | -1.15 | -1.58 | 4.13 | 0.54 | -5.95 | -4.02 | -4.25 | 1.34 | 1.29 | -7.07 | -3.58 | -4.06 | 1.69 |
Nuclear Periphery Foci | -6.20 | -11.61 | -1.73 | 2.68 | 6.13 | 2.14 | -6.82 | -4.47 | -6.56 | 5.86 | -3.38 | -13.57 | -5.25 | -2.20 | 8.99 |
Nucleolus | -1.81 | -8.79 | -3.79 | -1.57 | -2.69 | -0.74 | -0.78 | -5.20 | -3.36 | -5.24 | -1.63 | -4.65 | -6.12 | -3.80 | -5.20 |
Nucleus | -1.30 | -11.06 | -2.91 | -2.48 | -1.45 | -0.58 | -5.80 | -9.01 | -7.59 | -1.55 | -1.41 | -12.41 | -9.26 | -7.35 | -2.57 |
Peroxisomes | -2.51 | 2.09 | -3.69 | -3.53 | -3.71 | -2.69 | -1.91 | -8.35 | -8.11 | -5.79 | -3.44 | 0.82 | -8.70 | -8.20 | -8.74 |
Vacuole | -10.74 | -29.33 | -8.77 | -6.84 | 0.04 | -6.13 | -8.97 | -18.80 | -16.41 | 2.44 | -12.61 | -30.34 | -21.37 | -17.08 | 4.52 |
Vacuole Periphery | 3.34 | 6.79 | 12.43 | 9.05 | 8.20 | -1.74 | 2.30 | 7.13 | 8.88 | 1.23 | 2.75 | 4.73 | 14.64 | 12.13 | 9.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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