ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 6 | 3 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 13 | 7 | 10 | 14 | 10 | 10 | 15 | 7 | 11 | 13 | 13 | 14 |
Bud | 6 | 2 | 25 | 18 | 19 | 24 | – | 29 | 44 | 37 | 35 | 45 | 4 | 1 | 7 | 4 | 3 | 3 | 7 | 3 | 7 | 4 | 5 | 7 |
Bud Neck | 1 | 0 | 4 | 0 | 0 | 0 | – | 5 | 2 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 22 | 6 | 8 | 4 | 7 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | – | 3 | 9 | 5 | 5 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 2 | 5 | 7 | 4 | 2 | – | 1 | 1 | 4 | 1 | 2 | 1 | 10 | 3 | 7 | 4 | 4 | 3 | 0 | 3 | 1 | 1 | 0 |
Cytoplasm | 304 | 67 | 166 | 158 | 194 | 228 | – | 266 | 271 | 293 | 182 | 245 | 141 | 170 | 196 | 66 | 65 | 63 | 194 | 24 | 40 | 21 | 18 | 34 |
Endoplasmic Reticulum | 42 | 11 | 16 | 2 | 2 | 4 | – | 6 | 1 | 1 | 2 | 4 | 32 | 25 | 19 | 2 | 4 | 6 | 35 | 7 | 21 | 14 | 10 | 16 |
Endosome | 4 | 6 | 0 | 9 | 97 | 24 | – | 12 | 8 | 12 | 0 | 4 | 15 | 31 | 34 | 9 | 19 | 14 | 20 | 9 | 20 | 16 | 24 | 21 |
Golgi | 0 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 4 | 1 | 2 | 4 | 10 | 11 | 11 |
Mitochondria | 29 | 23 | 152 | 161 | 94 | 316 | – | 432 | 540 | 468 | 397 | 597 | 12 | 17 | 18 | 1 | 3 | 9 | 110 | 55 | 224 | 102 | 127 | 125 |
Nucleus | 1 | 2 | 0 | 2 | 0 | 2 | – | 8 | 4 | 4 | 4 | 6 | 1 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 2 | 0 | 0 | 0 | – | 4 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 1 | 2 | 3 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 2 | 11 | 4 | – | 3 | 5 | 3 | 2 | 6 | 0 | 7 | 4 | 2 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 0 |
SpindlePole | 2 | 0 | 5 | 3 | 11 | 11 | – | 32 | 24 | 34 | 18 | 28 | 2 | 1 | 1 | 3 | 3 | 6 | 4 | 0 | 4 | 2 | 2 | 4 |
Vac/Vac Membrane | 9 | 14 | 9 | 2 | 36 | 22 | – | 26 | 15 | 11 | 12 | 13 | 12 | 17 | 16 | 19 | 20 | 13 | 65 | 36 | 29 | 25 | 43 | 61 |
Unique Cell Count | 390 | 119 | 327 | 292 | 395 | 518 | 675 | 735 | 686 | 523 | 765 | 215 | 275 | 290 | 114 | 121 | 114 | 492 | 159 | 389 | 225 | 272 | 319 | |
Labelled Cell Count | 415 | 135 | 387 | 366 | 470 | 640 | 830 | 926 | 876 | 661 | 977 | 235 | 288 | 309 | 133 | 139 | 135 | 492 | 159 | 389 | 225 | 272 | 319 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.8 | 3.9 | 4.2 | 4.9 | 3.8 | – | 3.6 | 3.1 | 3.3 | 3.1 | 3.1 | 6.4 | 6.1 | 5.8 | 8.2 | 7.5 | 7.2 | 6.2 | 6.4 | 6.5 |
Std Deviation (1e-4) | 0.6 | 1.0 | 0.9 | 1.1 | 1.7 | 1.1 | – | 1.2 | 1.1 | 0.9 | 0.8 | 0.9 | 1.1 | 1.2 | 1.4 | 1.7 | 1.5 | 1.6 | 1.5 | 1.9 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.34 | -0.02 | – | -0.11 | -0.33 | -0.23 | -0.34 | -0.32 | 0.71 | 0.65 | 0.58 | 1.07 | 0.94 | 0.89 | 0.67 | 0.71 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 2.2 | 0 | 0 | 0 |
Bud | 0 | -1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.9 | -4.4 | -2.9 | -1.5 | -2.0 | -1.9 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | -0.4 | -1.9 | – | -3.4 | -4.2 | -2.4 | -4.6 | -5.9 | 3.4 | 2.7 | 4.2 | 1.3 | 0.6 | 0.8 |
Endoplasmic Reticulum | 0 | -3.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.0 | 2.0 | 0.9 | 0 | -0.7 | 0.2 |
Endosome | 0 | 9.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.8 | 6.2 | 6.4 | 0 | 7.3 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -6.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | -10.1 | -11.0 | -11.2 | -8.8 | -8.7 | -7.4 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | 3.5 | 1.1 | – | 0.9 | -0.7 | -1.2 | -0.4 | -1.1 | 1.7 | 2.1 | 1.7 | 5.2 | 5.3 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3173 | 2.3186 | 1.9475 | 1.6584 | 1.1739 | 1.9183 | 1.8776 | 2.0298 | 2.0864 | 1.9767 | 1.8696 | 1.7508 | 2.7262 | 3.6333 | 3.6466 | 3.1218 | 2.2893 | 3.1653 |
Actin | 0.0155 | 0.006 | 0.0072 | 0.0112 | 0.0047 | 0.0094 | 0.0312 | 0.0057 | 0.0303 | 0.0093 | 0.0304 | 0.0129 | 0.0269 | 0.011 | 0.0129 | 0.006 | 0.0042 | 0.0078 |
Bud | 0.0018 | 0.0031 | 0.0039 | 0.0169 | 0.0078 | 0.0038 | 0.0038 | 0.0051 | 0.008 | 0.0093 | 0.0056 | 0.0032 | 0.002 | 0.0067 | 0.005 | 0.0087 | 0.0043 | 0.0022 |
Bud Neck | 0.003 | 0.0043 | 0.0048 | 0.0027 | 0.0045 | 0.0132 | 0.0104 | 0.0027 | 0.0104 | 0.0066 | 0.0056 | 0.0154 | 0.0031 | 0.0053 | 0.0177 | 0.0045 | 0.0022 | 0.0208 |
Bud Periphery | 0.0039 | 0.0039 | 0.0085 | 0.0471 | 0.0164 | 0.0081 | 0.007 | 0.008 | 0.0118 | 0.0142 | 0.0169 | 0.0069 | 0.0028 | 0.006 | 0.0074 | 0.0235 | 0.0151 | 0.0081 |
Bud Site | 0.0151 | 0.036 | 0.0199 | 0.0123 | 0.0045 | 0.0065 | 0.0224 | 0.0628 | 0.0347 | 0.0068 | 0.0082 | 0.0034 | 0.0149 | 0.0872 | 0.0383 | 0.0069 | 0.005 | 0.003 |
Cell Periphery | 0.0036 | 0.0023 | 0.0044 | 0.0087 | 0.0022 | 0.0036 | 0.0027 | 0.0028 | 0.0032 | 0.0017 | 0.0053 | 0.0021 | 0.006 | 0.0048 | 0.0048 | 0.0048 | 0.0085 | 0.0032 |
Cytoplasm | 0.2863 | 0.4359 | 0.4108 | 0.3238 | 0.3258 | 0.3041 | 0.2133 | 0.3697 | 0.2727 | 0.2963 | 0.2541 | 0.3084 | 0.3186 | 0.4025 | 0.4572 | 0.2761 | 0.1848 | 0.332 |
Cytoplasmic Foci | 0.1112 | 0.0956 | 0.0967 | 0.08 | 0.1671 | 0.1315 | 0.1263 | 0.1167 | 0.1124 | 0.0974 | 0.1184 | 0.1238 | 0.1158 | 0.1076 | 0.0808 | 0.0869 | 0.1361 | 0.1211 |
Eisosomes | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0006 | 0.0003 | 0.0008 | 0.0003 | 0.0013 | 0.0004 | 0.0004 | 0.0002 | 0.001 | 0.0005 |
Endoplasmic Reticulum | 0.0387 | 0.0221 | 0.0249 | 0.0267 | 0.0135 | 0.0275 | 0.0206 | 0.0191 | 0.0267 | 0.0072 | 0.0118 | 0.0095 | 0.0354 | 0.0183 | 0.0331 | 0.0295 | 0.0162 | 0.021 |
Endosome | 0.2276 | 0.1818 | 0.1656 | 0.2146 | 0.1982 | 0.171 | 0.2521 | 0.186 | 0.1805 | 0.1931 | 0.1598 | 0.1949 | 0.1913 | 0.1571 | 0.1545 | 0.192 | 0.2287 | 0.2023 |
Golgi | 0.0403 | 0.0478 | 0.0486 | 0.0674 | 0.0459 | 0.0503 | 0.0789 | 0.0462 | 0.077 | 0.0727 | 0.0498 | 0.0659 | 0.0585 | 0.0536 | 0.0544 | 0.0843 | 0.1012 | 0.0772 |
Lipid Particles | 0.0154 | 0.005 | 0.005 | 0.0026 | 0.0041 | 0.0054 | 0.019 | 0.0041 | 0.0102 | 0.004 | 0.0131 | 0.0074 | 0.0165 | 0.0061 | 0.0062 | 0.0024 | 0.0029 | 0.0062 |
Mitochondria | 0.0638 | 0.0366 | 0.0857 | 0.1075 | 0.1126 | 0.1485 | 0.0925 | 0.0666 | 0.1186 | 0.172 | 0.1115 | 0.124 | 0.0531 | 0.0271 | 0.0499 | 0.1418 | 0.265 | 0.1108 |
None | 0.1159 | 0.0857 | 0.0779 | 0.0239 | 0.059 | 0.0807 | 0.0681 | 0.0685 | 0.0568 | 0.0522 | 0.1385 | 0.083 | 0.1099 | 0.076 | 0.0447 | 0.0905 | 0.0047 | 0.0589 |
Nuclear Periphery | 0.0056 | 0.0021 | 0.0036 | 0.0048 | 0.0018 | 0.0021 | 0.0085 | 0.0022 | 0.0029 | 0.0097 | 0.0128 | 0.0029 | 0.0064 | 0.0026 | 0.0024 | 0.0014 | 0.0011 | 0.0016 |
Nucleolus | 0.0008 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0006 | 0.0015 | 0.0027 | 0.0013 | 0.0007 | 0.0017 | 0.0008 | 0.0007 | 0.0007 | 0.0008 | 0.0003 | 0.0002 | 0.0003 |
Nucleus | 0.0031 | 0.0023 | 0.0022 | 0.0031 | 0.0024 | 0.0025 | 0.0054 | 0.0038 | 0.003 | 0.0033 | 0.0041 | 0.0028 | 0.002 | 0.0028 | 0.0023 | 0.0015 | 0.0007 | 0.0021 |
Peroxisomes | 0.0114 | 0.0032 | 0.0064 | 0.0038 | 0.0045 | 0.0101 | 0.0084 | 0.0044 | 0.0122 | 0.0066 | 0.0205 | 0.0107 | 0.0094 | 0.0047 | 0.0028 | 0.0077 | 0.0044 | 0.0049 |
Punctate Nuclear | 0.0047 | 0.001 | 0.001 | 0.0006 | 0.0017 | 0.0024 | 0.0038 | 0.0041 | 0.0022 | 0.0024 | 0.0093 | 0.0022 | 0.0032 | 0.0015 | 0.0013 | 0.0004 | 0.0003 | 0.0007 |
Vacuole | 0.0263 | 0.0211 | 0.0177 | 0.0316 | 0.0177 | 0.0135 | 0.0182 | 0.0149 | 0.019 | 0.0258 | 0.0163 | 0.0144 | 0.019 | 0.0155 | 0.0194 | 0.0227 | 0.0079 | 0.0112 |
Vacuole Periphery | 0.0054 | 0.0033 | 0.0044 | 0.0096 | 0.005 | 0.0048 | 0.0053 | 0.0033 | 0.0054 | 0.0083 | 0.0055 | 0.005 | 0.0031 | 0.0024 | 0.0039 | 0.0081 | 0.0056 | 0.0041 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 139.0846 | 118.8072 | 98.4113 | 81.4986 | 116.2122 | 169.8982 | 136.1892 | 96.1609 | 105.3115 | 144.9308 |
Translational Efficiency | 1.8995 | 1.8513 | 1.4545 | 2.3266 | 2.1334 | 1.6709 | 1.5017 | 2.3333 | 1.8324 | 1.8111 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1424 | 1759 | 160 | 1298 | 1721 | 1927 | 2048 | 1206 | 3145 | 3686 | 2208 | 2504 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 713.13 | 794.54 | 1015.15 | 1006.39 | 732.08 | 812.07 | 951.10 | 1053.12 | 723.50 | 803.70 | 955.74 | 1028.90 |
Standard Deviation | 110.50 | 111.68 | 125.73 | 153.67 | 94.08 | 102.54 | 133.18 | 152.21 | 102.28 | 107.36 | 133.69 | 154.74 |
Intensity Change Log 2 | — | 0.155955 | 0.509456 | 0.496952 | — | 0.149603 | 0.377596 | 0.524597 | — | 0.152741 | 0.444168 | 0.511022 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001737 | 0.001938 | 0.001926 | 0.033439 | 0.002119 | 0.001735 | 0.014378 | 0.038694 | 0.001946 | 0.001832 | 0.013475 | 0.035970 |
Bud Neck | 0.057303 | 0.035387 | 0.004273 | 0.012459 | 0.051401 | 0.043392 | 0.027891 | 0.011873 | 0.054073 | 0.039572 | 0.026179 | 0.012177 |
Bud Site | 0.029430 | 0.057436 | 0.016208 | 0.202101* | 0.031701 | 0.043589 | 0.188547* | 0.196239* | 0.030673 | 0.050197 | 0.176059* | 0.199278* |
Cell Periphery | 0.000837 | 0.000603 | 0.000547 | 0.000893 | 0.000418 | 0.000323 | 0.000358 | 0.000574 | 0.000608 | 0.000457 | 0.000372 | 0.000740 |
Cytoplasm | 0.218331* | 0.131819* | 0.308653* | 0.149324* | 0.185471* | 0.143643* | 0.250525* | 0.132048* | 0.200349* | 0.138000* | 0.254737* | 0.141004* |
Cytoplasmic Foci | 0.307915* | 0.309078* | 0.134100 | 0.197278* | 0.339664* | 0.293967* | 0.179478 | 0.237355* | 0.325289* | 0.301179* | 0.176189 | 0.216580* |
Eisosomes | 0.001355 | 0.000337 | 0.000182 | 0.000270 | 0.000876 | 0.000392 | 0.000191 | 0.000351 | 0.001093 | 0.000366 | 0.000191 | 0.000309 |
Endoplasmic Reticulum | 0.007658 | 0.002891 | 0.019334 | 0.015852 | 0.005374 | 0.002439 | 0.006911 | 0.006438 | 0.006408 | 0.002655 | 0.007811 | 0.011318 |
Endosome | 0.104979 | 0.113657 | 0.040943 | 0.079693 | 0.107534 | 0.128100 | 0.052865 | 0.085515 | 0.106377 | 0.121208 | 0.052001 | 0.082497 |
Golgi | 0.040491 | 0.079852 | 0.001375 | 0.062059 | 0.046955 | 0.061394 | 0.038838 | 0.115166* | 0.044028 | 0.070202 | 0.036123 | 0.087637 |
Lipid Particles | 0.035343 | 0.011199 | 0.014974 | 0.007987 | 0.028299 | 0.014793 | 0.004256 | 0.005414 | 0.031488 | 0.013078 | 0.005033 | 0.006747 |
Mitochondria | 0.051201 | 0.102372* | 0.000245 | 0.015640 | 0.051335 | 0.102602* | 0.053908 | 0.032646 | 0.051274 | 0.102492* | 0.050019 | 0.023831 |
Mitotic Spindle | 0.000305 | 0.007670 | 0.002372 | 0.023625 | 0.001912 | 0.006353 | 0.013581 | 0.021535 | 0.001184 | 0.006982 | 0.012769 | 0.022618 |
None | 0.001064 | 0.001236 | 0.001224 | 0.000358 | 0.000531 | 0.000679 | 0.000204 | 0.000128 | 0.000772 | 0.000945 | 0.000278 | 0.000247 |
Nuclear Periphery | 0.000410 | 0.000411 | 0.001784 | 0.000465 | 0.000272 | 0.000397 | 0.000173 | 0.000087 | 0.000334 | 0.000404 | 0.000290 | 0.000283 |
Nuclear Periphery Foci | 0.000321 | 0.000658 | 0.023128 | 0.002122 | 0.000329 | 0.000485 | 0.000803 | 0.000529 | 0.000325 | 0.000567 | 0.002420 | 0.001354 |
Nucleolus | 0.000654 | 0.000981 | 0.001121 | 0.000510 | 0.000551 | 0.001264 | 0.000264 | 0.000106 | 0.000598 | 0.001129 | 0.000326 | 0.000315 |
Nucleus | 0.017785 | 0.007574 | 0.142300* | 0.015841 | 0.015618 | 0.011961 | 0.009725 | 0.002179 | 0.016599 | 0.009867 | 0.019332 | 0.009261 |
Peroxisomes | 0.021746 | 0.028725 | 0.004999 | 0.029799 | 0.026866 | 0.024683 | 0.022279 | 0.032837 | 0.024548 | 0.026612 | 0.021027 | 0.031262 |
Vacuole | 0.089917 | 0.088591 | 0.278940* | 0.137472 | 0.093602 | 0.098972 | 0.118996 | 0.068583 | 0.091934 | 0.094018 | 0.130586 | 0.104293 |
Vacuole Periphery | 0.011218 | 0.017583 | 0.001371 | 0.012813 | 0.009173 | 0.018838 | 0.015831 | 0.011703 | 0.010099 | 0.018239 | 0.014783 | 0.012278 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.61 | -0.27 | -11.05 | -11.00 | -10.88 | 0.87 | -9.77 | -12.63 | -12.72 | -7.77 | 0.42 | -9.98 | -16.70 | -16.74 | -9.61 |
Bud Neck | 6.33 | 18.10 | 15.16 | 11.21 | -9.62 | 2.43 | 8.13 | 14.94 | 14.28 | 10.30 | 6.08 | 12.13 | 21.25 | 18.12 | 10.12 |
Bud Site | -8.41 | 4.31 | -24.89 | -20.20 | -26.66* | -4.30 | -31.44* | -25.16 | -22.99 | -1.28 | -9.09 | -31.44 | -35.35 | -30.54 | -3.76 |
Cell Periphery | 1.13 | 3.12 | -0.18 | -1.20 | -2.90 | 2.37 | 1.53 | -2.23 | -4.09 | -3.47 | 1.49 | 4.92 | -1.51 | -2.40 | -5.50 |
Cytoplasm | 12.19 | -4.13 | 8.18 | -3.20 | 7.50 | 6.64 | -9.47 | 7.06 | 1.16 | 15.71 | 13.20 | -8.89 | 10.48 | -1.51 | 17.38 |
Cytoplasmic Foci | -0.20 | 15.85 | 16.19 | 16.87 | -5.95 | 6.55 | 25.36 | 14.13 | 8.34 | -8.70 | 4.72 | 29.10 | 21.78 | 17.82 | -7.61 |
Eisosomes | 5.73 | 6.69 | 6.19 | 1.80 | -3.06 | 8.14 | 12.55 | 8.29 | 1.05 | -4.31 | 8.37 | 10.63 | 9.08 | 2.11 | -5.12 |
Endoplasmic Reticulum | 5.09 | -2.50 | -4.40 | -7.63 | 0.57 | 3.64 | -1.58 | -0.76 | -3.54 | 0.50 | 6.11 | -1.56 | -4.18 | -8.36 | -2.71 |
Endosome | -1.86 | 12.75 | 6.20 | 8.74 | -8.07 | -4.52 | 15.48 | 5.42 | 9.89 | -9.31 | -4.55 | 19.32 | 8.34 | 13.31 | -11.19 |
Golgi | -10.98 | 19.94 | -5.06 | 4.80 | -18.52 | -4.75 | 3.84 | -13.81 | -10.26 | -16.10 | -11.18 | 4.53 | -13.50 | -4.41 | -16.11 |
Lipid Particles | 12.05 | 8.46 | 13.76 | 2.92 | 3.35 | 7.91 | 16.58 | 14.73 | 7.98 | -1.90 | 14.17 | 22.21 | 19.94 | 7.87 | -3.36 |
Mitochondria | -10.09 | 17.65 | 11.32 | 20.00 | -10.70 | -10.54 | -0.23 | 5.01 | 15.43 | 5.75 | -14.57 | 0.90 | 11.17 | 25.18 | 9.28 |
Mitotic Spindle | -8.42 | -1.69 | -10.08 | -6.58 | -7.52 | -3.61 | -8.07 | -8.31 | -6.13 | -2.92 | -7.48 | -9.20 | -12.91 | -9.01 | -4.76 |
None | -0.33 | -0.13 | 2.26 | 1.78 | 1.81 | -0.73 | 2.40 | 2.82 | 4.28 | 2.05 | -0.61 | 3.27 | 3.23 | 2.84 | 0.22 |
Nuclear Periphery | -0.01 | -6.51 | -0.82 | -0.67 | 6.62 | -1.04 | 1.82 | 4.35 | 2.58 | 3.81 | -0.62 | 0.42 | 0.16 | 0.78 | -0.31 |
Nuclear Periphery Foci | -1.66 | -8.40 | -6.35 | -4.28 | 7.61 | -1.55 | -3.09 | -3.24 | -0.74 | 1.56 | -2.20 | -7.59 | -6.90 | -4.40 | 3.07 |
Nucleolus | -1.26 | -1.85 | 0.76 | 1.58 | 2.11 | -3.65 | 4.00 | 6.64 | 6.02 | 5.39 | -3.25 | 4.35 | 2.76 | 4.52 | 0.27 |
Nucleus | 8.10 | -8.57 | 0.07 | -5.11 | 8.54 | 2.57 | 3.67 | 11.79 | 9.31 | 9.24 | 7.06 | -2.21 | 4.93 | -0.51 | 5.49 |
Peroxisomes | -3.63 | 9.15 | -3.01 | -0.06 | -9.84 | 1.16 | 2.70 | -2.66 | -3.76 | -5.08 | -1.63 | 2.62 | -3.79 | -2.47 | -5.78 |
Vacuole | 0.19 | -11.44 | -8.96 | -9.11 | 8.02 | -1.12 | -6.74 | 4.06 | 4.96 | 10.05 | -0.66 | -10.73 | -4.90 | -4.34 | 5.09 |
Vacuole Periphery | -6.39 | 15.03 | -0.85 | 4.26 | -10.82 | -9.95 | -5.64 | -2.19 | 6.04 | 2.94 | -11.73 | -4.29 | -2.23 | 7.26 | 2.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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