Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.94 0.89 0.87 0.87 0.83 0.68 0.69 0.64 0.63 0.52 0.52 0.82 0.78 0.8 0.89 0.9 0.94 0.64 0.68 0.52 0.83 0.83 0.86
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.05 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.06 0 0.05 0.12 0.28 0.52 0.38 0.46 0.56 0.63 0.62 0 0 0 0 0 0.06 0.08 0.06 0.22 0 0.05 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.11 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0
Vac/Vac Membrane – 0 0.08 0.08 0.07 0 0.05 0.06 0 0 0.08 0 0.06 0.09 0.1 0.06 0.05 0.06 0.06 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 9 2 1 1
Bud – 0 2 0 1 2 19 7 12 20 15 6 0 0 0 0 0 0 0 6 13 2 5 2
Bud Neck – 0 1 0 0 0 0 3 1 1 1 0 1 2 0 0 0 0 0 1 2 0 1 1
Bud Site – 0 0 0 0 0 0 1 0 3 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 4 2 3 0 1 1 2 2 1 2 0 2 3 0 1 0 0 0 2 1 0 1
Cytoplasm – 134 408 212 316 391 389 339 304 388 264 310 175 373 382 16 52 15 75 114 244 230 226 257
Endoplasmic Reticulum – 2 1 1 1 0 0 0 0 0 1 2 3 5 1 0 2 3 5 0 6 0 3 1
Endosome – 0 20 5 10 5 3 4 3 2 0 5 1 4 5 0 2 1 0 2 9 2 3 2
Golgi – 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 5 0 1 0
Mitochondria – 8 7 12 42 132 298 185 217 347 323 372 0 1 1 0 2 1 9 10 105 10 13 10
Nucleus – 0 1 4 0 2 6 6 9 2 6 14 26 58 51 0 0 0 4 7 9 4 1 2
Nuclear Periphery – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0
Nucleolus – 0 1 2 0 1 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 3 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 3 0 2 5 8 10 6 10 8 8 0 0 1 0 0 0 8 6 31 5 3 4
Vac/Vac Membrane – 6 35 19 27 12 27 28 21 19 39 19 12 42 48 1 3 1 6 11 16 7 3 5
Unique Cell Count 142 461 244 363 469 572 490 475 615 509 596 214 476 477 18 58 16 118 168 472 279 272 300
Labelled Cell Count 150 488 257 402 550 751 586 577 796 660 741 218 488 493 18 62 21 118 168 472 279 272 300


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.0 4.0 3.8 3.7 3.1 3.1 3.2 3.1 2.8 2.7 2.7 5.2 5.5 5.3 4.9 5.4 5.7 5.0 5.2 5.5
Std Deviation (1e-4) – 0.9 1.1 1.8 1.1 1.3 1.3 1.5 1.5 0.8 0.9 0.9 0.9 1.1 1.1 1.2 1.3 2.8 1.4 1.6 1.6
Intensity Change (Log2) – – – -0.07 -0.1 -0.34 -0.36 -0.3 -0.33 -0.52 -0.55 -0.54 0.39 0.48 0.43 0.32 0.43 0.52 0.33 0.41 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8493 1.7825 1.7669 1.9453 1.5457 1.4093 -0.2753 -0.8956 0.0769 0.3851 0.4987 -0.6638 -1.3335 -0.9445 -0.255 0.4354 -0.375 -1.4472
Actin 0.0096 0.0051 0.0172 0.0004 0.0002 0.0005 0.0573 0.0029 0.0056 0.0023 0.0022 0.0043 0.0241 0.0003 0.0224 0.0187 0.0127 0.0053
Bud 0.0002 0.0049 0.0003 0.0001 0 0 0.0016 0.0018 0.0013 0.0004 0.0037 0.0008 0.0028 0.003 0.011 0.0126 0.0093 0.0055
Bud Neck 0.0005 0.0001 0.0021 0.0012 0.0001 0.0001 0.0074 0.0001 0.0006 0.0012 0.0053 0.0062 0.0012 0.0006 0.001 0.0026 0.001 0.0009
Bud Periphery 0.0002 0.0301 0.0004 0 0 0 0.0019 0.0041 0.0008 0.0004 0.0047 0.0013 0.0059 0.0019 0.0051 0.0053 0.004 0.0026
Bud Site 0.0009 0.0016 0.0132 0.0002 0 0 0.0232 0.0027 0.0024 0.0009 0.0109 0.0015 0.003 0.0137 0.0077 0.012 0.002 0.0005
Cell Periphery 0.0002 0.0005 0.0002 0 0 0 0.0006 0.0002 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0003 0.0003 0.0003 0.0002
Cytoplasm 0.117 0.2116 0.3129 0.5446 0.3693 0.0459 0.0319 0.1316 0.4039 0.4667 0.3634 0.2843 0.0924 0.1709 0.3817 0.386 0.337 0.1636
Cytoplasmic Foci 0.0399 0.0059 0.0047 0.003 0.0108 0.0025 0.1066 0.005 0.0162 0.0094 0.0923 0.0136 0.0494 0.0081 0.0148 0.0295 0.0243 0.0108
Eisosomes 0.0002 0.0004 0.0003 0 0 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0092 0.0034 0.0024 0.0027 0.0021 0.0004 0.0029 0.0013 0.005 0.0058 0.0065 0.0024 0.004 0.0032 0.0034 0.0043 0.0028 0.0017
Endosome 0.0139 0.0023 0.0015 0.0009 0.0073 0.0005 0.0625 0.0016 0.0243 0.0139 0.0498 0.016 0.029 0.0017 0.0058 0.0128 0.0163 0.0068
Golgi 0.0019 0.0003 0.0004 0 0.0002 0 0.0287 0.0002 0.006 0.0098 0.0433 0.0088 0.0122 0.0001 0.002 0.0041 0.0089 0.0007
Lipid Particles 0.0045 0.0005 0.0002 0.0001 0.0041 0.0001 0.0437 0.0003 0.0024 0.0039 0.0846 0.0016 0.0149 0.0003 0.0033 0.0032 0.0257 0.0008
Mitochondria 0.0007 0.0007 0.0004 0.0002 0.0008 0.0002 0.0187 0.0004 0.0079 0.0574 0.067 0.0061 0.0101 0.0009 0.0024 0.007 0.0065 0.0094
None 0.7581 0.7272 0.5488 0.3469 0.5813 0.9437 0.5489 0.8411 0.4246 0.2481 0.1679 0.623 0.6791 0.7538 0.3141 0.2368 0.3624 0.7532
Nuclear Periphery 0.0097 0.0009 0.0027 0.0027 0.0054 0.0004 0.0025 0.0009 0.0085 0.0123 0.0056 0.002 0.012 0.005 0.0078 0.0167 0.008 0.0068
Nucleolus 0.0005 0.0002 0.0005 0.0003 0.0009 0.0001 0.0024 0.0002 0.0004 0.0008 0.0016 0.0006 0.0011 0.001 0.0032 0.0026 0.0015 0.0008
Nucleus 0.0068 0.0018 0.084 0.093 0.0071 0.0039 0.0021 0.002 0.0688 0.1339 0.0317 0.015 0.0252 0.026 0.1752 0.1867 0.1389 0.015
Peroxisomes 0.0093 0.0004 0.0003 0.0001 0.0007 0.0002 0.0398 0.0005 0.008 0.0028 0.0297 0.0013 0.0137 0.0003 0.0048 0.0038 0.0093 0.0006
Punctate Nuclear 0.0149 0.0012 0.0066 0.0029 0.0078 0.001 0.0075 0.001 0.0066 0.026 0.0218 0.0078 0.0116 0.0066 0.031 0.049 0.0258 0.0133
Vacuole 0.0013 0.0007 0.0008 0.0005 0.0011 0.0002 0.0074 0.0019 0.005 0.0028 0.0039 0.0021 0.0052 0.002 0.0024 0.0033 0.0021 0.0011
Vacuole Periphery 0.0004 0.0001 0.0001 0.0001 0.0005 0 0.0019 0.0002 0.0014 0.0011 0.0036 0.0011 0.0025 0.0003 0.0005 0.0024 0.001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.0849 23.8144 17.6713 24.7442 38.4393 25.7662 31.846 31.9787 26.6809 37.2351
Translational Efficiency 0.7934 1.4653 1.0398 0.6492 0.6225 0.6812 1.0763 0.7656 0.6311 0.5125

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2047 385 686 1751 1650 1513 2346 187 3697 1898 3032 1938

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.89 754.47 864.17 912.88 688.82 723.71 879.17 972.88 694.95 729.95 875.78 918.67
Standard Deviation 78.79 99.17 90.47 120.08 79.11 100.66 101.81 126.13 79.12 101.12 99.56 121.97
Intensity Change Log 2 — 0.108335 0.304187 0.383297 — 0.071285 0.352015 0.498135 — 0.090077 0.328109 0.441401

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.000854 0.000306 0.001581 0.000075 0.000374 0.000202 0.000815 0.000109 0.000472 0.000225 0.001507
Bud Neck 0.010204 0.038800 0.004536 0.015023 0.008488 0.027263 0.009448 0.007280 0.009438 0.029603 0.008336 0.014276
Bud Site 0.003207 0.021836 0.006942 0.026248 0.002829 0.013378 0.009745 0.057887 0.003038 0.015094 0.009111 0.029301
Cell Periphery 0.000107 0.000187 0.000180 0.000319 0.000141 0.000270 0.000148 0.000196 0.000122 0.000253 0.000155 0.000307
Cytoplasm 0.502244* 0.340690* 0.167408* 0.212035* 0.526424* 0.416671* 0.249705* 0.241341* 0.513035* 0.401259* 0.231085* 0.214862*
Cytoplasmic Foci 0.179194 0.209783* 0.010287 0.006682 0.141526 0.202787* 0.004893 0.021829 0.162382 0.204206* 0.006113 0.008143
Eisosomes 0.000106 0.000156 0.000064 0.000050 0.000098 0.000152 0.000027 0.000108 0.000102 0.000153 0.000036 0.000055
Endoplasmic Reticulum 0.000728 0.002100 0.003904 0.008031 0.000803 0.000781 0.001923 0.008935 0.000762 0.001049 0.002371 0.008118
Endosome 0.006406 0.024884 0.002442 0.005487 0.003974 0.021351 0.001530 0.004021 0.005321 0.022068 0.001736 0.005345
Golgi 0.001089 0.003259 0.000069 0.005098 0.000892 0.004375 0.000099 0.000147 0.001001 0.004149 0.000092 0.004620
Lipid Particles 0.006762 0.006830 0.002254 0.001317 0.005045 0.010255 0.000706 0.003289 0.005996 0.009560 0.001056 0.001507
Mitochondria 0.004463 0.008803 0.000568 0.005856 0.003137 0.011246 0.001950 0.000597 0.003871 0.010751 0.001637 0.005349
Mitotic Spindle 0.005274 0.007619 0.001718 0.037691 0.002332 0.012417 0.005338 0.059520 0.003961 0.011443 0.004519 0.039798
None 0.004618 0.003957 0.005879 0.004566 0.006652 0.006010 0.006819 0.005400 0.005525 0.005593 0.006607 0.004647
Nuclear Periphery 0.000594 0.000772 0.001772 0.004157 0.000549 0.000932 0.001827 0.003008 0.000574 0.000899 0.001814 0.004046
Nuclear Periphery Foci 0.000497 0.001215 0.000620 0.001631 0.000507 0.000507 0.000274 0.002353 0.000502 0.000651 0.000353 0.001700
Nucleolus 0.001028 0.005111 0.001535 0.000626 0.000997 0.003016 0.000692 0.000421 0.001014 0.003441 0.000883 0.000606
Nucleus 0.238763* 0.201561* 0.754331* 0.580984* 0.271354* 0.188544* 0.677850* 0.346356* 0.253309* 0.191185* 0.695154* 0.558345*
Peroxisomes 0.004209 0.034171 0.000469 0.002750 0.003141 0.013358 0.000497 0.001071 0.003733 0.017580 0.000491 0.002588
Vacuole 0.029699 0.085617 0.034495 0.076467 0.020621 0.063481 0.025938 0.235249* 0.025647 0.067971 0.027874 0.091788
Vacuole Periphery 0.000673 0.001796 0.000222 0.003402 0.000415 0.002831 0.000389 0.000179 0.000558 0.002621 0.000352 0.003091

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.97 -5.20 -5.26 -0.51 -3.66 -2.92 -6.36 -8.20 -4.19 -6.81 -3.80 -6.06 -6.02 -3.08 -5.01
Bud Neck -5.74 2.72 -3.04 4.91 -4.94 -8.55 -0.38 0.19 8.67 0.57 -10.17 1.21 -3.83 7.72 -4.53
Bud Site -5.61 -1.63 -7.29 1.78 -3.07 -6.46 -3.76 -2.35 -0.71 -1.62 -8.25 -4.02 -7.85 -0.12 -4.39
Cell Periphery -5.39 -6.70 -6.90 -1.42 -1.53 -3.50 1.25 -2.46 2.38 -3.39 -4.54 -2.11 -6.20 1.22 -4.62
Cytoplasm 10.69 37.09 41.47 10.26 -3.72 11.18 37.53 25.42 17.40 4.40 14.29 51.18 52.42 27.63 3.78
Cytoplasmic Foci -2.47 39.14 41.04 19.81 5.15 -9.01 32.14 28.49 35.20 -3.54 -7.35 51.35 51.44 42.92 0.90
Eisosomes -3.89 3.92 6.87 7.58 2.55 -4.84 7.22 -2.74 2.09 -12.23 -6.55 11.21 5.82 13.19 -10.53
Endoplasmic Reticulum -4.45 -12.25 -25.11 -15.02 -6.99 0.05 -12.35 -8.37 -8.40 -6.71 -2.85 -19.01 -26.27 -25.05 -16.85
Endosome -5.26 6.44 6.00 6.39 -0.11 -11.65 6.43 1.25 12.17 -5.75 -12.05 10.20 5.66 13.96 -3.73
Golgi -2.94 8.74 -0.45 1.72 -1.91 -5.50 4.29 3.95 7.04 -3.11 -6.09 8.65 -0.45 3.46 -1.92
Lipid Particles -0.50 8.18 10.31 6.86 3.84 -5.00 9.37 3.82 7.35 -9.03 -4.37 14.14 12.12 10.05 -7.67
Mitochondria -1.57 5.00 1.65 2.25 -6.45 -5.29 2.15 3.57 7.55 2.59 -5.17 4.41 1.69 6.27 -3.53
Mitotic Spindle -0.95 0.97 -6.84 -5.37 -7.15 -4.61 -3.25 -3.06 -1.62 -2.44 -4.04 -1.66 -8.22 -4.49 -7.29
None 0.82 -1.08 -0.81 -1.51 0.47 0.66 1.07 2.20 1.35 1.59 -0.10 -0.48 1.17 0.86 1.70
Nuclear Periphery -2.77 -16.02 -21.80 -20.28 -14.67 -1.95 -24.32 -12.96 -9.88 -5.70 -2.15 -27.47 -23.59 -18.17 -14.06
Nuclear Periphery Foci -2.36 -2.59 -8.70 -1.39 -6.64 -0.02 1.63 -5.97 -6.02 -6.39 -1.85 0.39 -10.31 -8.20 -11.09
Nucleolus -2.64 -2.08 1.60 2.76 3.04 -4.96 2.82 2.93 5.93 0.51 -5.40 1.58 2.26 5.87 0.58
Nucleus 2.66 -49.47 -36.24 -24.89 16.60 10.42 -52.24 -4.70 -8.51 14.65 9.83 -74.00 -35.87 -40.06 19.25
Peroxisomes -5.80 9.27 5.90 6.46 -1.21 -7.89 5.12 5.37 11.01 0.51 -9.58 9.97 5.98 11.51 -1.32
Vacuole -8.83 -4.87 -24.90 -8.05 -18.52 -14.73 -10.62 -16.37 -14.21 -15.06 -15.84 -10.13 -29.28 -19.16 -24.14
Vacuole Periphery -4.78 4.51 -1.05 1.80 -2.47 -5.03 1.21 4.35 5.59 1.02 -5.28 2.97 -1.30 3.43 -2.25
Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (34%), nucleus (2%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Clb3

Clb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available