Standard name
Human Ortholog
Description Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function and for correct integration of membrane-multispanning proteins into the mitochondrial outer membrane; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 0 0.06 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.85 0.77 0.78 0.65 0.47 0.4 0.33 0.33 0.31 0.3 0.26 0.96 0.96 0.99 0.83 0.78 0.82 0.46 0.4 0.15 0.27 0.28 0.31
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0 0.05
Endosome 0 0 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.16 0.16 0.27 0.22 0.59 0.63 0.66 0.77 0.68 0.7 0.72 0 0 0 0.1 0.09 0.05 0.33 0.4 0.61 0.48 0.44 0.37
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0.07 0.08 0.09 0.07 0.08 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.11 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 4 3 13 1 3 1
Bud 1 3 9 4 5 28 15 32 30 27 38 29 1 0 0 2 3 4 0 0 6 0 2 2
Bud Neck 0 0 2 0 4 0 4 2 3 3 2 4 2 0 0 3 5 0 1 2 2 2 4 4
Bud Site 0 0 0 0 1 6 4 7 5 10 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 4 4 3 3 5 1 1 3 2 0 4 2 1 1 4 2 1 2 3 1 2 1
Cytoplasm 129 142 256 155 231 261 222 238 180 169 162 131 98 98 116 130 139 107 86 75 56 31 45 39
Endoplasmic Reticulum 2 3 0 0 0 2 0 1 1 0 1 0 1 3 0 12 12 5 1 1 9 1 0 5
Endosome 1 2 18 6 34 19 17 17 3 16 6 14 1 0 0 4 5 4 7 2 21 5 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 3 1 0 0 1
Mitochondria 27 27 53 53 78 329 347 472 418 374 382 363 0 0 0 16 16 6 61 76 232 56 70 46
Nucleus 0 0 0 1 1 2 7 8 2 7 2 7 1 0 0 1 3 2 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 0 2 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 2
Nucleolus 0 0 2 0 1 0 1 6 1 1 1 2 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 1 32 10 16 9 8 13 3 9 11 17 0 0 2 3 4 1 0 0 2 0 0 0
SpindlePole 1 1 8 6 26 38 42 63 38 45 45 46 0 0 0 0 5 5 3 3 11 2 6 1
Vac/Vac Membrane 5 3 7 3 29 17 17 18 6 15 19 15 0 2 1 6 8 5 9 8 5 4 17 12
Unique Cell Count 161 168 331 199 355 558 555 716 541 551 545 507 102 102 117 157 179 131 185 190 381 117 159 125
Labelled Cell Count 168 182 391 242 429 715 689 880 691 680 675 632 108 105 120 182 205 142 185 190 381 117 159 125


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.8 3.9 3.6 4.3 3.3 3.4 3.5 3.1 3.1 3.1 3.1 4.4 4.7 4.8 4.9 5.0 5.0 4.5 4.5 4.8
Std Deviation (1e-4) 0.5 1.1 1.6 1.7 2.6 1.8 3.1 1.4 1.4 0.6 1.0 0.7 0.8 0.7 1.0 1.5 1.3 1.1 1.5 0.9 2.0
Intensity Change (Log2) – – – -0.14 0.13 -0.27 -0.2 -0.19 -0.33 -0.33 -0.33 -0.34 0.15 0.25 0.29 0.33 0.34 0.34 0.2 0.17 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 1.5 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2141 -0.8864 -1.0036 -1.2049 -1.4268 -1.1998 0.5352 1.1006 0.8403 0.4557 0.4928 1.0371 -0.257 0.6134 -0.451 -0.0504 0.3049 -0.37
Actin 0.0038 0.0016 0.0025 0.0054 0.0052 0.0014 0.023 0.0019 0.0039 0.0012 0.0041 0.012 0.0468 0.0011 0.0085 0.0003 0.0102 0.001
Bud 0.0003 0.0003 0.0009 0.0003 0.0009 0.0006 0.0011 0.0061 0.003 0.0005 0.0022 0.0011 0.0045 0.0008 0.0011 0.0004 0.0005 0.0002
Bud Neck 0.0011 0.0005 0.0009 0.0007 0.0004 0.0013 0.0068 0.0013 0.0029 0.0046 0.0009 0.0114 0.0043 0.0019 0.0003 0.0007 0.0003 0.004
Bud Periphery 0.0007 0.0007 0.002 0.0011 0.0025 0.0009 0.003 0.0061 0.0084 0.0011 0.0104 0.0027 0.0107 0.0009 0.0021 0.0005 0.0009 0.0005
Bud Site 0.0015 0.0102 0.0048 0.0003 0.0023 0.0017 0.0063 0.0318 0.0078 0.0005 0.001 0.0007 0.0206 0.0309 0.0015 0.0013 0.0017 0.0001
Cell Periphery 0.0005 0.0006 0.0006 0.0002 0.0002 0.0003 0.0008 0.0012 0.0008 0.0002 0.0005 0.0005 0.0022 0.0011 0.0009 0.0002 0.0002 0.0004
Cytoplasm 0.1006 0.0966 0.1169 0.0906 0.1005 0.1495 0.1709 0.3546 0.2278 0.2236 0.2562 0.2671 0.0879 0.197 0.2296 0.1324 0.1098 0.2745
Cytoplasmic Foci 0.0579 0.0653 0.0837 0.0207 0.0415 0.065 0.0622 0.0689 0.0632 0.0702 0.0788 0.0795 0.0614 0.0571 0.0411 0.1698 0.036 0.03
Eisosomes 0.0008 0.0008 0.0006 0.0009 0.0004 0.0003 0.0005 0.0002 0.0004 0.0001 0.0003 0.0003 0.0023 0.0003 0.0005 0.0004 0.0004 0.0001
Endoplasmic Reticulum 0.0044 0.0025 0.0036 0.0033 0.0015 0.0021 0.0042 0.0024 0.0026 0.0022 0.0051 0.0025 0.0172 0.0021 0.0111 0.0019 0.0051 0.0114
Endosome 0.0249 0.0212 0.0386 0.0205 0.0303 0.0145 0.039 0.0248 0.0246 0.0428 0.0411 0.0298 0.052 0.0292 0.0397 0.0186 0.0699 0.0136
Golgi 0.0037 0.003 0.0047 0.0012 0.002 0.0027 0.006 0.0032 0.0046 0.0072 0.0047 0.0044 0.0157 0.0073 0.0056 0.0044 0.0381 0.0218
Lipid Particles 0.0107 0.0064 0.0062 0.0013 0.0025 0.0073 0.0077 0.0035 0.0039 0.0026 0.0027 0.0053 0.0314 0.0065 0.0089 0.0007 0.0312 0.0009
Mitochondria 0.0113 0.0066 0.0223 0.0047 0.0308 0.0162 0.0139 0.009 0.0216 0.0152 0.0225 0.02 0.0121 0.0085 0.0213 0.0022 0.0182 0.0038
None 0.7561 0.7694 0.6866 0.8336 0.7668 0.7214 0.6216 0.4634 0.6022 0.6107 0.4913 0.5394 0.5993 0.6413 0.5967 0.6453 0.6396 0.633
Nuclear Periphery 0.0031 0.0008 0.0015 0.0023 0.0009 0.0008 0.004 0.0009 0.0023 0.0009 0.0109 0.0039 0.0025 0.0035 0.002 0.0008 0.005 0.0005
Nucleolus 0.0011 0.0008 0.0008 0.0012 0.0004 0.0006 0.0011 0.0018 0.0011 0.0005 0.0013 0.0005 0.0027 0.0006 0.0003 0.0008 0.0006 0.0001
Nucleus 0.0023 0.0022 0.0016 0.0022 0.0012 0.0015 0.0028 0.005 0.0029 0.002 0.0054 0.0015 0.0016 0.0025 0.0012 0.0015 0.0013 0.0005
Peroxisomes 0.0036 0.0054 0.0144 0.001 0.0032 0.0075 0.0113 0.0038 0.0076 0.0073 0.0449 0.0105 0.0093 0.0032 0.0148 0.0113 0.0201 0.0006
Punctate Nuclear 0.0073 0.0018 0.0024 0.0037 0.0017 0.0023 0.0067 0.0025 0.0034 0.0017 0.0082 0.003 0.0042 0.001 0.0005 0.0014 0.0046 0.0005
Vacuole 0.0036 0.0026 0.0038 0.0042 0.0034 0.0017 0.006 0.0067 0.0038 0.0035 0.0058 0.003 0.0099 0.0027 0.0099 0.0047 0.003 0.0019
Vacuole Periphery 0.0007 0.0006 0.0009 0.0005 0.001 0.0005 0.0014 0.0009 0.0011 0.0011 0.0018 0.0011 0.0014 0.0005 0.0025 0.0005 0.0033 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.418 5.7886 10.3089 10.1225 7.556 16.8267 9.3645 11.8508 11.1349 12.3246
Translational Efficiency 1.0553 1.6474 0.6564 0.8682 0.9267 0.4828 1.2495 0.8732 0.9562 0.7269

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2134 1708 207 1345 1798 1171 1487 2405 3932 2879 1694 3750

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 666.52 727.96 818.48 831.18 695.26 676.29 824.79 823.73 679.66 706.94 824.02 826.40
Standard Deviation 73.92 96.37 92.65 142.01 71.48 89.31 89.93 108.08 74.21 96.95 90.29 121.40
Intensity Change Log 2 — 0.127211 0.296299 0.318513 — -0.039911 0.246474 0.244619 — 0.044306 0.271076 0.281259

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000129 0.000472 0.000312 0.000866 0.000323 0.000532 0.000120 0.000895 0.000218 0.000496 0.000143 0.000885
Bud Neck 0.047243 0.058011 0.005258 0.012618 0.050478 0.067604 0.008093 0.027852 0.048722 0.061913 0.007747 0.022388
Bud Site 0.006456 0.022108 0.005205 0.043849 0.010993 0.021091 0.008462 0.042893 0.008531 0.021695 0.008064 0.043236
Cell Periphery 0.000143 0.000243 0.000201 0.000896 0.000090 0.000343 0.000099 0.000140 0.000119 0.000283 0.000111 0.000411
Cytoplasm 0.308739* 0.218918* 0.189439* 0.444115* 0.274631* 0.157299* 0.492752* 0.479802* 0.293142* 0.193855* 0.455688* 0.467002*
Cytoplasmic Foci 0.290821* 0.292418* 0.050154 0.049867 0.359190* 0.265014* 0.025755 0.075781 0.322084* 0.281271* 0.028736 0.066487
Eisosomes 0.000204 0.000212 0.000114 0.000066 0.000224 0.000265 0.000031 0.000075 0.000213 0.000234 0.000041 0.000072
Endoplasmic Reticulum 0.001316 0.001265 0.004302 0.002010 0.001197 0.001618 0.001545 0.001111 0.001262 0.001409 0.001882 0.001433
Endosome 0.039544 0.051231 0.004880 0.007471 0.034624 0.086159 0.002435 0.010312 0.037294 0.065438 0.002734 0.009293
Golgi 0.005862 0.022808 0.000131 0.000962 0.011122 0.029844 0.000108 0.001760 0.008267 0.025670 0.000111 0.001474
Lipid Particles 0.024016 0.016568 0.006486 0.002029 0.016537 0.036528 0.001225 0.002356 0.020596 0.024687 0.001868 0.002239
Mitochondria 0.024804 0.073515 0.000544 0.003078 0.033538 0.116495* 0.000811 0.005406 0.028798 0.090997 0.000778 0.004571
Mitotic Spindle 0.003850 0.007532 0.000310 0.043856 0.001388 0.010856 0.004185 0.024436 0.002725 0.008884 0.003711 0.031401
None 0.002430 0.003690 0.005236 0.013009 0.000831 0.005821 0.003583 0.003196 0.001699 0.004556 0.003785 0.006716
Nuclear Periphery 0.000805 0.000841 0.002521 0.002686 0.000409 0.001275 0.001717 0.001152 0.000624 0.001017 0.001815 0.001702
Nuclear Periphery Foci 0.000274 0.000344 0.001258 0.001345 0.000207 0.000561 0.000544 0.000379 0.000243 0.000432 0.000632 0.000725
Nucleolus 0.002704 0.004294 0.002164 0.001196 0.001173 0.004823 0.000545 0.001115 0.002004 0.004509 0.000742 0.001144
Nucleus 0.137450* 0.102942 0.660874* 0.234645* 0.098642 0.069581 0.396925* 0.193149* 0.119704 0.089373 0.429179* 0.208032*
Peroxisomes 0.014645 0.034394 0.001534 0.003493 0.018263 0.040574 0.000429 0.012880 0.016299 0.036907 0.000564 0.009513
Vacuole 0.085380 0.080801 0.058748 0.129492 0.083152 0.074116 0.050317 0.113777 0.084361 0.078082 0.051347 0.119413
Vacuole Periphery 0.003187 0.007393 0.000329 0.002450 0.002986 0.009602 0.000320 0.001535 0.003095 0.008291 0.000321 0.001863

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.08 -4.12 -5.91 -2.87 -3.25 -1.46 4.82 -4.46 -1.74 -6.24 -4.28 1.78 -7.34 -3.31 -7.89
Bud Neck -2.95 15.01 15.08 16.02 -3.04 -3.67 16.66 8.86 9.94 -11.65 -4.59 22.26 14.92 16.54 -10.82
Bud Site -9.90 0.30 -11.02 -4.52 -8.04 -4.56 1.98 -9.77 -4.68 -10.37 -10.03 0.84 -14.85 -6.50 -13.08
Cell Periphery -2.65 -2.94 -7.75 -6.01 -6.77 -10.74 -0.96 -4.71 9.20 -3.26 -6.84 1.24 -7.91 -2.00 -8.12
Cytoplasm 11.41 9.15 -8.82 -18.10 -13.82 14.02 -19.51 -20.92 -34.49 0.79 17.14 -15.99 -21.88 -37.10 -0.81
Cytoplasmic Foci -0.22 33.84 47.71 42.16 0.81 10.76 57.25 47.37 28.51 -15.66 7.16 73.90 64.59 48.81 -13.74
Eisosomes -0.71 5.88 18.50 14.34 4.68 -2.36 15.72 12.24 14.67 -12.64 -2.06 24.44 20.50 20.37 -9.44
Endoplasmic Reticulum 0.31 -5.59 -7.72 -7.58 3.94 -1.57 -5.91 -1.88 0.74 5.18 -1.01 -8.09 -6.08 -3.29 4.51
Endosome -4.20 18.12 19.75 20.78 0.02 -12.25 19.50 14.74 19.77 -8.84 -11.83 28.83 23.71 27.60 -8.95
Golgi -10.51 13.90 12.17 14.27 -4.02 -8.22 15.94 13.60 13.12 -6.24 -13.00 20.95 17.44 19.19 -7.24
Lipid Particles 4.82 12.09 18.68 14.51 5.67 -8.17 17.88 16.56 14.70 -7.46 -3.13 24.51 24.38 19.80 -1.73
Mitochondria -11.69 13.85 12.60 18.91 -5.80 -13.41 17.03 14.75 19.03 -9.62 -17.53 21.58 18.85 26.44 -10.67
Mitotic Spindle -2.24 2.05 -8.61 -7.23 -9.46 -5.48 -3.13 -10.85 -4.21 -7.75 -5.19 -1.52 -13.12 -8.46 -11.05
None -1.97 -1.43 -4.88 -3.77 -2.17 -3.59 -8.78 -4.96 2.02 0.78 -4.24 -4.79 -5.90 -0.52 -2.61
Nuclear Periphery -0.21 -9.49 -4.35 -4.12 -0.45 -3.63 -18.18 -13.30 -1.77 3.08 -2.74 -17.65 -7.86 -5.00 -0.17
Nuclear Periphery Foci -2.20 -4.75 -7.69 -7.07 1.00 -2.62 -6.90 -4.95 0.94 3.72 -3.28 -8.33 -9.06 -4.20 0.60
Nucleolus -3.33 1.88 6.43 8.38 3.32 -6.84 4.96 -1.06 6.55 -6.60 -7.23 8.21 4.79 10.28 -5.37
Nucleus 6.12 -26.70 -15.27 -18.97 19.94 5.59 -40.35 -20.48 -23.54 23.73 7.69 -44.01 -23.84 -28.47 27.37
Peroxisomes -9.57 12.84 10.62 16.83 -1.03 -8.79 18.21 5.22 11.61 -9.52 -12.89 22.56 8.59 18.19 -9.69
Vacuole 1.00 0.37 -16.32 -16.33 -10.84 2.16 2.84 -13.99 -13.96 -15.47 2.12 3.24 -21.41 -21.36 -20.58
Vacuole Periphery -8.73 16.10 4.26 10.23 -3.06 -10.74 16.76 8.35 13.65 -5.49 -13.70 21.97 8.98 17.69 -5.78
Standard name
Human Ortholog
Description Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function and for correct integration of membrane-multispanning proteins into the mitochondrial outer membrane; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
Localization
Cell Percentages mitochondrion (14%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Crd1

Crd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Crd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available