Standard name
Human Ortholog
Description Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.21 0.11 0 0.05 0.05 0 0.06 0.07 0.05 0 0 0.17 0.12 0.13 0.17 0.15 0.33 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.28 0.24 0.25 0.22 0.39 0.32 0.21 0.29 0.3 0.33 0.3 0.13 0.06 0.09 0.13 0.08 0.07 0 0 0 0 0 0
Nucleus 0.95 0.92 0.89 0.89 0.85 0.76 0.75 0.69 0.69 0.67 0.68 0.68 0.88 0.85 0.74 0.81 0.83 0.74 0.9 0.9 0.86 0.88 0.83 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.11 0.15 0.24 0.26 0.47 0.47 0.46 0.42 0.52 0.5 0.49 0.1 0.12 0.21 0.09 0.06 0.07 0 0 0.06 0 0.06 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 5
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 0 0 0 0 0 2 1 1 2 2 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 24 47 41 12 23 23 30 52 44 42 25 23 38 42 42 38 27 42 0 1 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 1 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 60 63 86 68 98 178 276 177 175 243 218 228 28 21 28 31 15 9 0 0 1 0 2 1
Nucleus 175 207 323 246 381 343 640 589 418 532 449 509 197 286 244 187 150 93 146 176 291 166 259 231
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 3 5
Nucleolus 22 24 54 66 118 213 402 389 251 413 331 371 23 41 69 20 11 9 6 6 20 7 18 27
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 3 5 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 1 5 1 3 7 6 3 6 3 0 2 6 5 8 4 2 2 7 8 17 46
Unique Cell Count 185 226 364 275 447 452 851 851 602 800 658 754 223 337 329 230 180 126 164 197 340 190 313 333
Labelled Cell Count 282 342 504 394 625 758 1352 1218 896 1234 1032 1136 286 395 395 283 214 159 164 197 340 190 313 333


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 123.5 125.1 126.5 110.5 94.5 72.2 62.3 62.2 56.6 49.8 45.5 44.5 131.2 122.5 111.6 167.8 161.9 177.1 116.5 128.8 138.7
Std Deviation (1e-4) 37.2 42.2 51.9 38.1 40.7 28.3 24.5 29.0 24.5 22.3 20.2 19.4 56.7 63.6 56.2 68.6 75.9 88.0 48.1 53.6 60.3
Intensity Change (Log2) – – – -0.2 -0.42 -0.81 -1.02 -1.02 -1.16 -1.35 -1.48 -1.51 0.05 -0.05 -0.18 0.41 0.36 0.48 -0.12 0.03 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.1 -3.2 -3.3 -5.3 -3.1 -2.1 -3.7 -4.6 -5.5 2.0 0.5 0.6 1.8 1.2 5.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 -0.6 4.8 3.1 -1.1 1.8 2.4 3.2 2.3 -3.3 -6.4 -5.4 -3.0 -4.3 -4.0
Nucleus 0.3 -1.5 -4.7 -5.3 -7.2 -6.9 -8.0 -7.3 -7.6 -0.1 -1.5 -5.0 -2.5 -1.8 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.9 4.0 9.8 10.7 10.2 8.7 11.9 11.2 11.1 -1.6 -1.0 2.1 -2.2 -3.0 -2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 191.5251 181.4523 182.3865 159.3778 157.2807 163.6701 185.7691 226.1555 201.2919 194.9701 201.2373 203.5379 199.2887 206.4551 191.6894 190.8351 183.4769 185.5389
Actin 0.0046 0 0 0 0 0 0.0004 0 0.0095 0.0001 0 0 0.0101 0 0 0 0 0.0007
Bud 0 0 0 0 0 0 0.0005 0.0001 0.0002 0 0 0 0.0001 0 0 0 0 0
Bud Neck 0.0003 0 0 0 0.0006 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Bud Periphery 0 0 0 0 0.0001 0 0.0003 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.0004 0.0001 0.0004 0 0 0 0.0002 0 0 0 0 0
Cell Periphery 0.0001 0.0002 0 0.0002 0.002 0.0001 0.0013 0.0001 0.0003 0.0001 0.0002 0.0002 0.0003 0 0.0001 0 0.0003 0.0001
Cytoplasm 0.0006 0.0001 0 0.0001 0 0 0.0023 0.0001 0.0005 0.0001 0 0 0.0002 0 0 0 0 0
Cytoplasmic Foci 0.0002 0.0001 0 0.0003 0 0 0.0003 0.0004 0.0114 0.0003 0 0 0.0058 0 0.0001 0 0 0.0001
Eisosomes 0.0001 0 0 0.0003 0 0 0.0001 0 0.0001 0 0 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0 0 0 0 0 0.0007 0 0.0003 0 0 0 0.0001 0 0 0 0 0.0001
Endosome 0.0028 0 0 0 0 0 0.003 0 0.0014 0.0001 0 0 0.0033 0 0 0 0 0.0011
Golgi 0.0005 0 0 0 0 0 0.0001 0 0.0017 0 0 0 0.0017 0 0 0 0 0.0004
Lipid Particles 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0.0019 0.0001 0 0 0.007 0 0 0 0 0.0009
Mitochondria 0.0026 0.0025 0.0008 0.0171 0.0062 0.0024 0.0075 0.0025 0.0083 0.0118 0.0136 0.0011 0.0082 0.0009 0.0018 0.001 0.0129 0.0014
None 0.001 0.0001 0 0.0005 0 0 0.0009 0.0003 0.0016 0.0001 0 0 0.0008 0 0 0 0 0.0001
Nuclear Periphery 0.0021 0.001 0.0002 0.0005 0.0001 0 0.0281 0.0004 0.0055 0.002 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0009
Nucleolus 0.062 0.0562 0.0271 0.067 0.176 0.075 0.0806 0.0299 0.0495 0.0678 0.2025 0.0758 0.0845 0.0443 0.042 0.051 0.2497 0.0644
Nucleus 0.9201 0.9389 0.9719 0.9127 0.8105 0.9219 0.8689 0.9652 0.8971 0.9153 0.7825 0.922 0.8679 0.9545 0.9555 0.9478 0.7351 0.9288
Peroxisomes 0.0001 0 0 0.0001 0 0 0.0002 0 0.0025 0 0 0 0.0076 0 0 0 0 0.0001
Punctate Nuclear 0.0004 0 0 0 0 0 0.001 0 0.0058 0 0 0 0.0005 0 0 0 0 0.0003
Vacuole 0.0002 0.0002 0 0.0002 0.0021 0.0001 0.0014 0.0002 0.0005 0.0004 0.0001 0.0002 0.0004 0 0.0001 0 0.0002 0.0001
Vacuole Periphery 0.0003 0.0004 0.0001 0.0008 0.002 0.0003 0.0017 0.0006 0.0011 0.0014 0.0009 0.0005 0.0006 0.0002 0.0002 0.0001 0.0015 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 672.6347 624.0339 652.8207 632.214 544.4544 820.2184 790.7731 735.7074 743.5 690.9137
Translational Efficiency 1.0691 1.0794 0.9726 0.9566 1.0298 1.1357 1.1228 1.0344 0.9816 1.0136

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
94 379 610 1572 1398 2223 1083 371 1492 2602 1693 1943

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 6218.99 9432.55 7007.42 5813.62 6813.69 8527.62 5844.55 6662.34 6776.22 8659.43 6263.54 5975.68
Standard Deviation 2845.25 2730.01 1977.59 2425.87 2259.46 2418.06 1550.54 1998.66 2305.31 2486.53 1805.19 2373.86
Intensity Change Log 2 — 0.600968 0.172203 -0.097244 — 0.323707 -0.221344 -0.032407 — 0.462667 -0.020144 -0.062983

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000852 0.002539 0.000607 0.000698 0.000710 0.001677 0.000547 0.000728 0.000719 0.001802 0.000569 0.000704
Bud Neck 0.001575 0.000866 0.002943 0.003791 0.001188 0.001876 0.002356 0.004254 0.001212 0.001729 0.002567 0.003879
Bud Site 0.000297 0.000059 0.000286 0.000943 0.000154 0.000245 0.000244 0.000395 0.000163 0.000218 0.000259 0.000839
Cell Periphery 0.000077 0.000041 0.000040 0.000533 0.000226 0.000490 0.000082 0.000038 0.000217 0.000424 0.000067 0.000438
Cytoplasm 0.000337 0.000013 0.000025 0.001667 0.000286 0.000270 0.000100 0.000276 0.000289 0.000233 0.000073 0.001401
Cytoplasmic Foci 0.002489 0.000201 0.000289 0.003347 0.000347 0.000828 0.000396 0.001086 0.000482 0.000737 0.000357 0.002915
Eisosomes 0.000270 0.001543 0.000338 0.000123 0.001531 0.003559 0.000539 0.000128 0.001452 0.003265 0.000467 0.000124
Endoplasmic Reticulum 0.000263 0.000265 0.000094 0.000341 0.000181 0.000610 0.000140 0.000108 0.000186 0.000559 0.000124 0.000296
Endosome 0.000038 0.000001 0.000001 0.000798 0.000001 0.000003 0.000001 0.000950 0.000004 0.000003 0.000001 0.000827
Golgi 0.000059 0.000005 0.000008 0.001083 0.000004 0.000007 0.000006 0.000481 0.000008 0.000007 0.000007 0.000968
Lipid Particles 0.000375 0.000002 0.000001 0.000199 0.000001 0.000001 0.000001 0.000030 0.000024 0.000001 0.000001 0.000167
Mitochondria 0.001991 0.001720 0.001363 0.003518 0.000841 0.001022 0.001144 0.002374 0.000914 0.001124 0.001223 0.003300
Mitotic Spindle 0.000026 0.000066 0.000005 0.001208 0.000000 0.000089 0.000004 0.000492 0.000002 0.000086 0.000004 0.001071
None 0.000698 0.000726 0.000331 0.003388 0.000793 0.000526 0.000646 0.000546 0.000787 0.000555 0.000532 0.002845
Nuclear Periphery 0.000275 0.000641 0.000018 0.000425 0.000027 0.000382 0.000016 0.000087 0.000043 0.000420 0.000017 0.000361
Nuclear Periphery Foci 0.000622 0.000020 0.000000 0.000125 0.000001 0.000005 0.000001 0.000042 0.000040 0.000008 0.000001 0.000109
Nucleolus 0.095145 0.158237 0.014339 0.014682 0.014501 0.041972 0.008487 0.023295 0.019582 0.058907 0.010595 0.016326
Nucleus 0.872357* 0.813648* 0.975077* 0.957387* 0.974351* 0.939364* 0.982004* 0.957795* 0.967925* 0.921052* 0.979508* 0.957465*
Peroxisomes 0.020984 0.009686 0.003783 0.002046 0.003302 0.002099 0.002805 0.003911 0.004416 0.003204 0.003157 0.002402
Vacuole 0.000813 0.003004 0.000236 0.001882 0.001289 0.003669 0.000384 0.001802 0.001259 0.003572 0.000331 0.001867
Vacuole Periphery 0.000458 0.006715 0.000214 0.001817 0.000266 0.001306 0.000099 0.001183 0.000278 0.002094 0.000141 0.001696

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.10 1.88 1.15 7.20 -1.39 -10.86 3.60 -0.42 8.68 -2.62 -12.57 3.36 0.06 12.39 -3.08
Bud Neck 2.18 -3.57 -5.42 -10.73 -2.86 -5.53 -9.26 -6.50 -4.98 -4.10 -4.67 -11.72 -11.18 -8.66 -5.53
Bud Site 2.87 0.39 -3.05 -4.56 -3.47 -2.03 -3.88 -2.75 -0.79 -1.73 -1.65 -4.85 -4.31 -3.45 -3.75
Cell Periphery 1.00 0.99 -2.24 -2.52 -2.52 -2.63 2.54 3.41 5.31 2.51 -2.35 2.87 -1.35 -0.10 -2.35
Cytoplasm 1.57 1.53 -2.09 -3.10 -3.08 -0.89 0.61 0.05 1.02 -0.91 -0.74 0.84 -2.26 -1.52 -3.03
Cytoplasmic Foci 1.82 1.75 -0.74 -3.82 -3.71 -1.40 -0.32 -0.96 -0.17 -0.87 -0.91 0.92 -3.57 -2.89 -3.72
Eisosomes -2.99 -1.90 4.11 3.34 11.59 -4.47 4.52 6.60 8.58 7.13 -4.52 4.86 6.66 9.04 9.17
Endoplasmic Reticulum 0.05 1.26 -0.43 -0.87 -4.26 -9.08 1.74 2.97 10.47 1.28 -8.72 3.01 -2.20 4.40 -3.61
Endosome 1.44 1.45 -3.00 -3.18 -3.18 -2.75 0.47 -1.14 -1.14 -1.14 0.53 1.53 -3.20 -3.20 -3.21
Golgi 1.69 1.57 -2.04 -2.17 -2.16 -2.24 -2.48 -1.27 -1.26 -1.27 0.03 0.22 -2.35 -2.35 -2.35
Lipid Particles 1.12 1.12 0.53 -1.93 -1.94 -2.64 -3.10 -1.09 -1.06 -1.08 1.07 1.10 -1.65 -2.00 -2.00
Mitochondria 0.60 1.38 -1.92 -2.61 -3.13 -3.26 -4.35 -5.62 -4.95 -4.44 -3.47 -4.44 -4.39 -3.99 -3.78
Mitotic Spindle -0.58 1.12 -3.17 -3.03 -3.23 -1.95 -1.66 -1.21 -0.97 -1.20 -2.09 -1.06 -3.42 -3.14 -3.42
None -0.08 1.72 -3.12 -3.08 -3.66 1.14 0.43 1.08 -0.19 0.31 1.06 0.98 -2.93 -3.35 -3.31
Nuclear Periphery -1.39 1.41 -0.62 0.86 -2.51 -2.87 0.94 -2.68 2.37 -3.70 -3.43 1.63 -2.40 0.35 -2.62
Nuclear Periphery Foci 1.08 1.11 0.91 -2.44 -2.95 -2.70 -0.20 -1.46 -1.24 -1.46 0.91 1.11 -1.25 -2.90 -3.14
Nucleolus -2.44 3.63 3.60 10.34 -0.34 -8.20 2.91 -1.95 3.15 -3.23 -10.03 3.55 0.88 11.29 -3.03
Nucleus 1.92 -3.86 -3.14 -9.15 3.91 9.13 -2.89 2.56 -2.34 3.69 10.47 -3.75 2.93 -7.26 6.63
Peroxisomes 1.41 2.20 2.41 3.96 3.13 2.33 0.83 -0.94 -2.46 -1.52 1.62 1.74 2.69 1.52 1.70
Vacuole -1.73 1.71 -1.67 0.92 -3.39 -5.44 4.29 -0.32 1.33 -0.97 -5.50 4.77 -1.10 2.98 -3.19
Vacuole Periphery -2.24 1.39 -2.12 1.74 -2.58 -5.77 4.63 -1.22 0.20 -1.44 -4.16 2.99 -2.73 0.69 -3.01
Standard name
Human Ortholog
Description Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Lys21

Lys21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available