ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.43 0.75 0.88 0.93 0.9 0.76 0.62 0.53 0.24 0.23 0.15 0.19 0.84 0.9 0.87 – 0.34 0.35 0.27 0.46 0.56 0.26 0.27 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0.13 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.14 0 0 0.12 0.16 0.29 0.17 0.75 0.56 0.66 0.6 0 0 0 – 0.08 0 0 0.06 0.12 0.05 0.06 0.06
Nucleus 0.29 0.09 0 0 0 0 0.07 0.17 0.19 0.28 0.33 0.32 0.13 0 0.07 – 0.36 0.37 0.31 0.13 0 0.25 0.14 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 – 0 0.06 0.08 0.07 0.06 0.12 0.13 0.1
Vac/Vac Membrane 0.24 0.07 0 0 0 0.07 0.06 0.07 0 0 0 0 0.05 0.07 0.07 – 0.22 0.17 0.18 0.15 0.1 0.17 0.21 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 8 5 1 1 4
Bud 0 0 1 0 1 3 6 2 25 11 14 22 0 0 0 – 0 0 5 6 5 8 11 18
Bud Neck 1 2 9 2 5 5 7 17 5 12 10 7 0 2 2 – 0 0 5 6 1 2 2 6
Bud Site 0 0 0 0 1 0 0 1 15 4 10 13 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 1
Cytoplasm 111 340 290 285 260 338 373 236 161 155 89 128 185 302 391 – 123 132 98 261 224 78 88 124
Endoplasmic Reticulum 0 1 1 0 1 0 0 0 0 0 1 0 1 0 0 – 25 36 0 0 3 0 2 3
Endosome 8 3 6 4 0 10 13 21 3 10 10 5 1 3 3 – 46 39 11 11 6 6 10 9
Golgi 2 0 0 1 0 0 1 0 0 0 0 0 0 0 0 – 0 3 1 0 0 0 1 1
Mitochondria 26 64 2 8 35 70 172 76 498 379 399 407 1 1 0 – 27 11 14 36 47 14 21 20
Nucleus 76 39 11 4 5 13 40 76 127 191 198 218 28 14 32 – 128 140 111 72 15 73 47 36
Nuclear Periphery 0 1 0 0 0 0 1 0 0 1 0 3 0 0 0 – 0 1 1 2 2 1 2 0
Nucleolus 0 0 0 1 0 0 2 0 8 6 2 10 0 0 0 – 2 1 3 3 2 10 10 10
Peroxisomes 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 – 0 6 0 1 0 0 0 0
SpindlePole 1 0 4 4 2 5 24 26 13 32 25 28 0 1 1 – 6 21 28 42 24 35 41 38
Vac/Vac Membrane 63 32 12 9 6 31 33 32 24 24 19 24 12 23 33 – 79 66 66 86 38 51 70 68
Unique Cell Count 260 456 328 305 288 445 600 449 667 682 608 684 221 336 448 359 378 362 571 401 299 332 366
Labelled Cell Count 288 482 338 318 316 476 673 489 879 825 778 865 228 346 462 437 456 362 571 401 299 332 366


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.6 4.9 4.6 3.9 4.1 4.0 4.4 3.4 3.9 3.6 3.9 4.8 4.8 4.6 4.8 6.3 6.2 7.9 7.4 6.5
Std Deviation (1e-4) 0.6 0.5 1.0 1.3 1.3 1.2 1.2 1.0 0.9 0.9 0.8 0.9 1.4 1.7 1.1 1.6 2.4 1.7 8.1 6.5 4.6
Intensity Change (Log2) – – – -0.09 -0.33 -0.27 -0.29 -0.16 -0.53 -0.34 -0.44 -0.33 -0.04 -0.05 -0.11 -0.05 0.35 0.34 0.69 0.59 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 7.1 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8505 3.5693 3.1793 3.6025 3.5196 3.4988 7.2001 5.0867 5.2699 3.9599 5.7807 5.7527 2.4968 2.7099 2.3548 2.4409 2.1868 2.5209
Actin 0.0713 0.0006 0.0201 0.0668 0.0006 0.0025 0.0842 0.0016 0.0207 0.0038 0.0057 0.0018 0.0068 0.0002 0.0074 0.0002 0.0003 0.0007
Bud 0.0007 0.0002 0.0002 0.0016 0.0022 0.0003 0.0061 0.0046 0.0009 0.0146 0.0004 0.0004 0.001 0.0077 0.001 0 0.0005 0.0002
Bud Neck 0.005 0.0009 0.0022 0.0024 0.0012 0.0018 0.0058 0.0058 0.0015 0.0023 0.0026 0.0064 0.0063 0.0003 0.0021 0.0003 0.0018 0.0029
Bud Periphery 0.0012 0.0002 0.0003 0.0051 0.0007 0.0004 0.0041 0.0073 0.0007 0.0214 0.0008 0.0009 0.0009 0.0021 0.0036 0 0.0005 0.0002
Bud Site 0.0097 0.0011 0.0009 0.0019 0.0004 0.0001 0.0216 0.0147 0.0034 0.0043 0.0014 0.0003 0.0079 0.0118 0.0014 0.0001 0.0002 0.0002
Cell Periphery 0.0005 0.0001 0.0001 0.0002 0.0001 0 0.0005 0.0006 0.0001 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0 0 0
Cytoplasm 0.1249 0.166 0.1992 0.144 0.1644 0.2411 0.1332 0.2141 0.1809 0.2366 0.151 0.218 0.1325 0.1154 0.1248 0.1288 0.124 0.1597
Cytoplasmic Foci 0.0266 0.0106 0.0114 0.0067 0.005 0.0074 0.0299 0.0302 0.0093 0.0036 0.0066 0.0201 0.0161 0.0035 0.0046 0.0053 0.0033 0.0123
Eisosomes 0.0013 0.0003 0.0004 0.0004 0.0001 0.0001 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0077 0.0021 0.0059 0.0041 0.0025 0.0025 0.0141 0.0066 0.0022 0.0026 0.003 0.0037 0.0029 0.0016 0.002 0.0007 0.0008 0.0014
Endosome 0.0175 0.0015 0.0087 0.0102 0.0024 0.0041 0.025 0.0033 0.0034 0.0017 0.0211 0.0053 0.0044 0.0012 0.0026 0.0015 0.0015 0.0026
Golgi 0.0063 0.0001 0.0027 0.0023 0.0001 0.0005 0.0068 0.0005 0.0029 0.0001 0.0034 0.0004 0.0009 0.0001 0.001 0.0001 0.0001 0.0001
Lipid Particles 0.0134 0.0004 0.0032 0.0025 0.0003 0.0013 0.0192 0.001 0.0007 0.0002 0.0167 0.001 0.0018 0.0004 0.003 0.0001 0.0002 0.0003
Mitochondria 0.0044 0.0006 0.002 0.0025 0.001 0.0007 0.0033 0.0015 0.0019 0.0019 0.0042 0.0014 0.0014 0.0006 0.0044 0.0006 0.0009 0.0006
None 0.4217 0.6821 0.4832 0.4431 0.6309 0.4896 0.3965 0.5669 0.5807 0.3502 0.4592 0.5408 0.5897 0.7569 0.634 0.59 0.5525 0.5876
Nuclear Periphery 0.0233 0.0035 0.015 0.03 0.0058 0.0094 0.047 0.0066 0.0084 0.0077 0.0659 0.0116 0.0085 0.0034 0.0072 0.0066 0.0041 0.0061
Nucleolus 0.0047 0.0021 0.002 0.0031 0.0024 0.0027 0.0063 0.0022 0.0026 0.004 0.0121 0.0018 0.0034 0.0022 0.0025 0.0028 0.0066 0.0031
Nucleus 0.2087 0.1099 0.1915 0.2169 0.1613 0.2022 0.1371 0.0895 0.1108 0.322 0.2035 0.1352 0.1739 0.0771 0.1659 0.205 0.2566 0.163
Peroxisomes 0.0112 0.0005 0.0076 0.0028 0.0002 0.0004 0.0163 0.0013 0.0009 0.0005 0.007 0.0005 0.0056 0.0002 0.0044 0.0001 0.0002 0.0006
Punctate Nuclear 0.0363 0.0156 0.0417 0.0508 0.0174 0.0313 0.0347 0.0372 0.0666 0.0196 0.029 0.0469 0.0337 0.0139 0.0265 0.0572 0.0449 0.0571
Vacuole 0.0023 0.0014 0.0014 0.0014 0.0011 0.001 0.0052 0.0035 0.0009 0.0019 0.0031 0.0026 0.0015 0.0012 0.001 0.0004 0.0008 0.0011
Vacuole Periphery 0.001 0.0002 0.0004 0.001 0.0002 0.0003 0.0027 0.0006 0.0003 0.0006 0.0032 0.0007 0.0003 0.0002 0.0003 0.0001 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7065 4.504 5.0132 6.9595 8.1656 7.5534 10.5311 6.9157 8.6237 8.5717
Translational Efficiency 1.1038 2.0571 1.4306 0.7434 0.913 0.9273 0.506 0.4771 0.5956 0.8885

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
642 992 2375 1855 2635 2407 2396 2006 3277 3399 4771 3861

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 634.88 676.28 754.10 834.63 639.81 797.28 767.42 807.58 638.84 761.97 760.79 820.58
Standard Deviation 65.85 92.36 92.62 142.98 74.51 93.71 103.65 115.27 72.92 108.32 98.54 130.03
Intensity Change Log 2 — 0.091137 0.248272 0.394653 — 0.317443 0.262373 0.335962 — 0.209159 0.255367 0.365492

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000371 0.000207 0.001301 0.000050 0.000239 0.000203 0.000381 0.000066 0.000278 0.000205 0.000823
Bud Neck 0.007741 0.016853 0.009391 0.023646 0.006223 0.019076 0.006786 0.017493 0.006520 0.018427 0.008083 0.020449
Bud Site 0.001735 0.005302 0.010661 0.040648 0.001131 0.008601 0.009675 0.018594 0.001250 0.007638 0.010165 0.029190
Cell Periphery 0.000199 0.000168 0.000176 0.000340 0.000097 0.000178 0.000124 0.000190 0.000117 0.000175 0.000150 0.000262
Cytoplasm 0.266630* 0.317373* 0.211218* 0.242014* 0.369474* 0.318920* 0.198503* 0.217330* 0.349326* 0.318468* 0.204833* 0.229189*
Cytoplasmic Foci 0.179788 0.111322 0.002777 0.008668 0.060121 0.070047 0.001894 0.001821 0.083565 0.082093 0.002334 0.005110
Eisosomes 0.000129 0.000078 0.000019 0.000033 0.000044 0.000059 0.000019 0.000017 0.000061 0.000064 0.000019 0.000025
Endoplasmic Reticulum 0.001339 0.002480 0.001883 0.004568 0.000791 0.001079 0.002357 0.003297 0.000899 0.001488 0.002121 0.003907
Endosome 0.004438 0.010313 0.000865 0.010371 0.001310 0.004001 0.000804 0.001184 0.001923 0.005843 0.000834 0.005598
Golgi 0.001314 0.001728 0.000080 0.011815 0.000427 0.001040 0.000137 0.000430 0.000601 0.001241 0.000109 0.005900
Lipid Particles 0.020023 0.009325 0.000499 0.002567 0.006444 0.006080 0.000493 0.000396 0.009104 0.007027 0.000496 0.001439
Mitochondria 0.007421 0.010053 0.001525 0.010333 0.003721 0.009853 0.001357 0.002342 0.004446 0.009911 0.001441 0.006181
Mitotic Spindle 0.002084 0.006402 0.006597 0.130530* 0.002073 0.011301 0.008246 0.011108 0.002075 0.009872 0.007425 0.068484
None 0.006382 0.013127 0.006851 0.011733 0.006218 0.007488 0.007973 0.009005 0.006250 0.009134 0.007415 0.010316
Nuclear Periphery 0.002805 0.002489 0.002215 0.005365 0.002050 0.002072 0.002124 0.002310 0.002198 0.002194 0.002170 0.003778
Nuclear Periphery Foci 0.001185 0.001723 0.000278 0.001848 0.000254 0.000561 0.000281 0.000358 0.000437 0.000900 0.000279 0.001074
Nucleolus 0.004797 0.009383 0.000980 0.002010 0.001644 0.003881 0.001048 0.000852 0.002261 0.005487 0.001014 0.001408
Nucleus 0.466058* 0.441024* 0.731148* 0.426825* 0.529363* 0.513458* 0.743515* 0.692913* 0.516961* 0.492318* 0.737358* 0.565072*
Peroxisomes 0.010120 0.008529 0.000166 0.001281 0.002367 0.006909 0.000236 0.000164 0.003886 0.007382 0.000201 0.000700
Vacuole 0.014756 0.029983 0.012081 0.054656 0.005940 0.014272 0.013691 0.018572 0.007667 0.018857 0.012890 0.035908
Vacuole Periphery 0.000922 0.001973 0.000382 0.009449 0.000256 0.000887 0.000534 0.001243 0.000386 0.001204 0.000459 0.005185

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.28 -3.64 -6.55 -4.27 -5.74 -4.07 -8.01 -8.98 -3.23 -2.92 -5.83 -10.29 -9.67 -5.61 -6.43
Bud Neck -4.09 -1.41 -7.12 -1.23 -7.45 -8.81 -2.03 -8.67 0.13 -7.44 -9.82 -3.18 -12.52 -0.52 -10.43
Bud Site -4.84 -6.28 -10.57 -8.36 -5.53 -7.18 -5.49 -8.17 -2.77 -3.05 -8.34 -8.60 -13.51 -7.13 -6.11
Cell Periphery 0.81 1.12 0.32 -0.81 -1.29 -5.38 -1.83 -2.31 1.05 -1.68 -4.40 -1.50 -3.09 0.43 -2.05
Cytoplasm -4.14 7.46 7.42 12.85 0.15 7.10 30.28 27.82 19.56 -1.52 5.09 31.23 29.62 23.64 -0.91
Cytoplasmic Foci 7.28 21.85 21.69 21.01 -2.48 -3.29 26.35 26.57 26.55 1.49 0.27 32.14 31.96 33.34 -1.08
Eisosomes 6.62 16.39 12.46 8.42 -13.54 -4.13 5.85 7.02 11.34 1.27 -1.37 14.07 9.62 12.02 -8.96
Endoplasmic Reticulum -3.70 -4.48 -12.65 -9.51 -11.55 -2.46 -12.91 -17.85 -15.50 -3.28 -5.16 -15.67 -23.85 -19.13 -10.51
Endosome -5.17 8.87 1.50 5.92 -6.06 -7.95 3.09 2.20 8.82 -0.23 -9.82 8.10 -1.32 8.05 -5.82
Golgi -0.84 2.97 -2.00 -1.52 -3.75 -2.87 3.36 1.57 3.81 -1.46 -3.18 4.64 -2.52 -0.88 -3.89
Lipid Particles 5.21 10.30 9.61 8.71 -2.20 0.23 11.60 11.87 13.02 1.74 2.87 15.31 13.75 14.02 -1.88
Mitochondria -1.19 3.27 1.10 3.75 -5.94 -6.35 5.56 3.46 8.56 -3.42 -6.44 6.15 1.33 8.12 -6.76
Mitotic Spindle -2.52 -3.16 -14.86 -13.91 -13.73 -5.36 -4.33 -5.93 -0.71 -2.35 -5.92 -5.51 -15.87 -11.63 -13.12
None -5.01 0.57 -3.55 2.43 -5.04 -1.51 -0.99 -2.43 -0.32 -1.24 -3.96 -0.39 -4.45 0.47 -4.65
Nuclear Periphery 0.33 0.02 -4.97 -8.27 -10.12 -0.06 -4.37 -6.86 -4.30 -4.16 -0.03 -3.13 -10.32 -8.82 -10.87
Nuclear Periphery Foci -1.63 3.19 0.24 2.27 -7.51 -2.34 -2.58 -4.31 0.56 -0.16 -3.57 0.34 -4.58 1.05 -5.58
Nucleolus -3.94 7.13 5.32 7.00 -4.44 -4.72 2.07 3.81 6.67 2.37 -6.83 6.21 4.50 9.14 -2.24
Nucleus 1.36 -22.11 -6.42 -8.81 21.12 2.10 -30.25 -22.27 -22.81 6.09 3.43 -37.72 -16.66 -19.50 19.19
Peroxisomes 1.26 8.65 8.33 9.30 -2.95 -6.08 6.42 6.86 10.08 1.89 -5.34 10.50 10.14 13.10 -1.67
Vacuole -6.07 -2.16 -20.44 -15.40 -19.79 -9.19 -12.60 -14.90 -10.35 -5.40 -11.74 -14.19 -26.24 -20.11 -19.09
Vacuole Periphery -2.55 2.03 -2.99 -1.26 -4.45 -5.19 -0.96 -1.53 0.67 -1.00 -6.06 0.35 -4.23 -1.45 -4.32
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (50%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YDL129W

YDL129W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydl129w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available