Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0.05 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.08 0.06 0.1 0.09 0 0.05 0 0 0 0 0.22 0.27 0.35 0.1 0.08 0.08 0 0 0 0.09 0.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.16 0.1 0.21 0 0 0 0 0 0 0 0 0.06 0.09 0.05 0.05 0 0.05 0 0.08 0 0.12 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.59 0.36 0.26 0.43 0.32 0.71 0.72 0.65 0.67 0.78 0.74 0.76 0 0 0 0.12 0.1 0.13 0 0 0.05 0 0.05 0.11
Nucleus 0.38 0.63 0.52 0.47 0.33 0.32 0.38 0.42 0.52 0.44 0.54 0.45 0.66 0.58 0.49 0.68 0.64 0.61 0.44 0.55 0.36 0.41 0.21 0.13
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0.08 0.11 0.07 0.13 0.07 0.07 0.09 0.07 0.06 0 0 0 0.06 0.07 0.08 0.06 0.06 0.1 0.08 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.09 0 0.13 0.06 0.09 0.07 0 0.05 0 0 0.07 0.05 0.07 0 0.05 0.07 0.09 0 0.16 0.1 0.13 0.14
Vac/Vac Membrane 0.14 0.13 0.06 0.13 0.08 0.08 0 0.05 0.05 0 0 0.06 0 0.09 0.07 0.11 0.14 0.15 0.22 0.19 0.15 0.2 0.27 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 3 5 11 5 3 5 4 6 0 0 0 0 0 0 2 1 2 0 4 5
Bud Neck 0 0 0 0 0 1 0 1 0 0 0 0 3 2 0 0 0 0 1 0 0 2 3 4
Bud Site 0 0 0 1 2 10 10 15 6 14 14 5 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 8 11 9 19 16 14 17 8 13 6 11 60 108 159 27 20 20 4 6 3 18 35 40
Endoplasmic Reticulum 1 1 0 0 1 0 0 0 0 0 0 0 2 2 2 9 7 8 0 0 0 0 1 2
Endosome 2 2 21 14 38 7 2 9 1 0 0 4 2 25 39 13 13 9 12 6 13 7 44 47
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 1 0 1 1 0 2 0
Mitochondria 112 47 34 62 58 125 228 205 162 275 168 190 5 3 2 34 26 31 7 3 9 8 18 43
Nucleus 73 82 69 68 60 56 120 132 126 155 123 112 179 229 223 191 161 148 107 89 64 85 76 54
Nuclear Periphery 3 0 0 1 0 0 1 5 0 5 3 2 0 1 0 11 3 4 10 1 5 0 1 4
Nucleolus 7 1 6 11 20 13 40 21 17 33 16 14 1 2 3 18 18 19 14 10 17 16 17 13
Peroxisomes 0 0 1 0 2 2 2 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
SpindlePole 1 1 12 5 24 11 27 23 4 16 2 2 20 19 34 8 12 16 21 6 29 22 47 58
Vac/Vac Membrane 27 17 8 19 14 14 5 16 13 9 9 16 12 37 32 31 35 36 52 29 26 42 95 108
Unique Cell Count 190 131 132 145 184 176 316 315 242 353 226 250 271 396 457 282 250 242 243 161 179 211 361 408
Labelled Cell Count 233 159 162 190 241 260 460 450 340 525 345 362 285 429 495 345 298 292 243 161 179 211 361 408


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.3 4.3 4.0 4.5 3.6 3.5 3.6 3.5 3.4 3.2 3.4 5.4 5.6 5.4 6.6 6.7 6.3 5.5 5.8 6.1
Std Deviation (1e-4) 0.5 0.9 0.9 0.9 1.8 1.4 1.2 1.0 0.8 0.9 1.0 1.3 1.1 1.3 1.4 1.5 1.7 1.6 1.3 1.5 1.6
Intensity Change (Log2) – – – -0.11 0.07 -0.27 -0.31 -0.27 -0.29 -0.34 -0.44 -0.34 0.34 0.39 0.33 0.62 0.64 0.56 0.36 0.44 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.6 2.3 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.49 1.7129 1.5144 1.0838 1.0141 1.7475 0.6767 0.5644 0.36 1.205 0.538 0.281 -0.8269 -0.7569 -0.7457 -0.6946 -0.8174 -0.8081
Actin 0.005 0.0001 0.0002 0.014 0.0004 0.0008 0.0306 0.0025 0.0073 0.0614 0.028 0.011 0.0036 0.0001 0.0037 0.0096 0.0081 0.0047
Bud 0.0001 0 0 0.0001 0 0.0001 0.0014 0.0002 0.0004 0.0019 0.0031 0.0003 0.0005 0 0.0001 0.0094 0.0002 0
Bud Neck 0.0014 0.0005 0.0006 0.001 0.0025 0.0029 0.0058 0.001 0.0014 0.0032 0.0055 0.0039 0.001 0.0005 0.0008 0.0018 0.0012 0.0031
Bud Periphery 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0022 0.0003 0.0004 0.0041 0.0051 0.0004 0.0004 0 0.0003 0.0035 0.0005 0.0001
Bud Site 0.0007 0.0002 0.0003 0.0003 0.0001 0.0003 0.0102 0.0014 0.0037 0.0053 0.0118 0.0005 0.0005 0.0003 0.0004 0.0034 0.0004 0.0001
Cell Periphery 0.0001 0 0 0.0001 0 0 0.0007 0.0001 0.0001 0.0006 0.0008 0.0001 0.0001 0 0.0001 0.0004 0.0001 0
Cytoplasm 0.0268 0.0203 0.0093 0.0033 0.0038 0.0169 0.0051 0.0069 0.0028 0.0038 0.004 0.0035 0.0107 0.0011 0.0012 0.0053 0.0078 0.0039
Cytoplasmic Foci 0.002 0.0003 0.0005 0.0022 0.0002 0.0005 0.0293 0.0007 0.0032 0.0091 0.0054 0.0032 0.0032 0.0001 0.0017 0.0062 0.0157 0.0002
Eisosomes 0.0002 0 0 0.0005 0 0 0.0003 0 0 0.0002 0.0004 0.0001 0.0001 0 0 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0006 0.0006 0.0005 0.0003 0.0005 0.0032 0.0012 0.0002 0.0017 0.0043 0.0045 0.0024 0.0003 0.0003 0.0007 0.0011 0.0002
Endosome 0.0045 0.0009 0.0022 0.0047 0.0007 0.0008 0.0348 0.0014 0.0024 0.0754 0.0233 0.0085 0.0116 0.0002 0.0021 0.0033 0.0116 0.0007
Golgi 0.0006 0 0.0002 0.0061 0 0.0005 0.0124 0.0003 0.0015 0.0181 0.018 0.0114 0.0012 0 0.0015 0.0014 0.0061 0.0003
Lipid Particles 0.0018 0.0001 0.0033 0.0138 0.0001 0.0003 0.034 0.0002 0.0015 0.005 0.0118 0.0058 0.0052 0.0001 0.0034 0.0033 0.0248 0.0009
Mitochondria 0.0014 0.0002 0.0004 0.0129 0.0009 0.003 0.0155 0.0008 0.0094 0.0561 0.0737 0.0069 0.0034 0.0002 0.0029 0.0015 0.008 0.0006
None 0.011 0.0278 0.003 0.016 0.0014 0.0044 0.003 0.0009 0.0007 0.0019 0.0006 0.0004 0.0162 0.0002 0.0014 0.0071 0.018 0.0045
Nuclear Periphery 0.0297 0.0311 0.0411 0.0221 0.1186 0.0071 0.0449 0.0299 0.0153 0.0236 0.0169 0.0086 0.0479 0.0255 0.0408 0.0101 0.0248 0.0092
Nucleolus 0.0631 0.0554 0.0877 0.179 0.0705 0.1033 0.073 0.047 0.0627 0.1344 0.1093 0.0693 0.0645 0.0566 0.0685 0.1685 0.1062 0.0867
Nucleus 0.7665 0.8255 0.777 0.6583 0.6181 0.8007 0.6143 0.8591 0.8534 0.5239 0.5959 0.8074 0.7833 0.8828 0.8098 0.6946 0.6122 0.8444
Peroxisomes 0.0024 0 0.0002 0.0075 0 0.0003 0.0256 0.0003 0.0014 0.0036 0.0049 0.005 0.0007 0 0.0032 0.0022 0.0036 0.0003
Punctate Nuclear 0.0792 0.0357 0.0722 0.0564 0.1808 0.0571 0.0422 0.0439 0.0309 0.0466 0.0665 0.0451 0.0371 0.0314 0.0566 0.0656 0.1464 0.0395
Vacuole 0.001 0.0009 0.0005 0.0004 0.0003 0.0003 0.0076 0.0015 0.0008 0.0106 0.007 0.0028 0.0037 0.0003 0.0005 0.0014 0.0012 0.0003
Vacuole Periphery 0.0006 0.0004 0.0005 0.0006 0.0011 0.0001 0.0037 0.0006 0.0004 0.0093 0.0039 0.001 0.0029 0.0002 0.0007 0.0004 0.002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.4279 5.711 3.6324 9.9213 15.9746 15.5066 5.362 5.846 6.2485 14.2168
Translational Efficiency 0.8932 0.8699 1.1132 0.7738 0.5397 0.9826 0.832 0.5978 0.9637 0.913

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
177 198 233 907 1531 2118 750 80 1708 2316 983 987

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.56 794.03 937.28 811.47 693.50 735.24 939.92 883.84 691.23 740.27 939.29 817.34
Standard Deviation 84.39 100.69 90.08 119.82 90.57 92.20 102.95 97.23 90.20 94.40 100.05 119.79
Intensity Change Log 2 — 0.241677 0.480964 0.273021 — 0.084319 0.438642 0.349889 — 0.163879 0.459618 0.312585

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000231 0.001751 0.001679 0.001121 0.000145 0.000227 0.000557 0.001494 0.000154 0.000358 0.000823 0.001151
Bud Neck 0.002189 0.005882 0.004328 0.009453 0.003523 0.007094 0.004599 0.002339 0.003385 0.006991 0.004534 0.008876
Bud Site 0.000547 0.002315 0.001986 0.007957 0.000826 0.002332 0.000885 0.006427 0.000797 0.002331 0.001146 0.007833
Cell Periphery 0.000109 0.000241 0.000116 0.000206 0.000139 0.000162 0.000066 0.000165 0.000136 0.000169 0.000078 0.000202
Cytoplasm 0.100277* 0.084554 0.007764 0.033139 0.192740* 0.090292 0.015413 0.014342 0.183158* 0.089801 0.013600 0.031616
Cytoplasmic Foci 0.066966 0.060979 0.000749 0.001640 0.032281 0.027619 0.000545 0.001353 0.035876 0.030471 0.000593 0.001617
Eisosomes 0.000092 0.000150 0.000090 0.000044 0.000057 0.000049 0.000040 0.000090 0.000060 0.000058 0.000052 0.000048
Endoplasmic Reticulum 0.002668 0.008689 0.017145 0.014377 0.002220 0.002744 0.007573 0.016609 0.002266 0.003252 0.009842 0.014558
Endosome 0.002239 0.004947 0.001249 0.002842 0.002385 0.004405 0.000528 0.001170 0.002370 0.004451 0.000699 0.002706
Golgi 0.000407 0.001316 0.000167 0.001617 0.000451 0.001712 0.000059 0.000126 0.000446 0.001678 0.000085 0.001496
Lipid Particles 0.023280 0.018411 0.001704 0.001010 0.008189 0.011621 0.000757 0.001978 0.009753 0.012201 0.000981 0.001088
Mitochondria 0.004430 0.012992 0.001859 0.002818 0.003007 0.011564 0.000702 0.001501 0.003155 0.011686 0.000976 0.002711
Mitotic Spindle 0.002144 0.003805 0.000542 0.016916 0.003056 0.014156 0.001204 0.000142 0.002962 0.013271 0.001047 0.015557
None 0.009559 0.012587 0.002136 0.008534 0.027794 0.010397 0.003640 0.004752 0.025904 0.010584 0.003284 0.008228
Nuclear Periphery 0.004523 0.004483 0.002001 0.005283 0.003042 0.008420 0.001600 0.001360 0.003196 0.008083 0.001695 0.004965
Nuclear Periphery Foci 0.016959 0.015809 0.002642 0.004039 0.009289 0.006821 0.000875 0.002632 0.010084 0.007589 0.001294 0.003925
Nucleolus 0.054322 0.032174 0.017599 0.009978 0.065275 0.049309 0.005826 0.009370 0.064140 0.047844 0.008616 0.009929
Nucleus 0.699017* 0.699017* 0.920053* 0.860670* 0.640341* 0.741140* 0.948044* 0.897408* 0.646421* 0.737538* 0.941410* 0.863648*
Peroxisomes 0.005866 0.015987 0.000797 0.000472 0.001612 0.004187 0.000233 0.000593 0.002053 0.005196 0.000367 0.000482
Vacuole 0.003794 0.013031 0.015119 0.014869 0.003293 0.005036 0.006728 0.035805 0.003344 0.005719 0.008717 0.016566
Vacuole Periphery 0.000382 0.000880 0.000275 0.003016 0.000336 0.000714 0.000126 0.000344 0.000341 0.000728 0.000162 0.002799

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.24 -9.49 -10.73 0.95 4.70 -1.41 -10.37 -6.80 -6.57 -3.05 -3.40 -13.79 -15.00 -11.20 -0.74
Bud Neck -3.47 -6.68 -11.00 -6.04 -5.71 -5.43 -4.28 0.49 4.60 3.90 -6.06 -6.10 -10.43 -7.25 -6.86
Bud Site -4.78 -5.22 -5.11 -3.23 -2.56 -2.84 -3.56 -1.75 -1.19 -1.42 -3.33 -5.92 -5.18 -3.90 -4.10
Cell Periphery -3.92 -3.21 -1.97 1.79 -0.04 -1.34 5.88 -1.79 -0.64 -3.95 -2.01 3.53 -1.48 -0.13 -2.61
Cytoplasm 0.96 7.45 5.55 5.20 -7.01 13.29 26.33 24.14 15.86 -0.78 13.03 27.55 23.81 15.42 -6.37
Cytoplasmic Foci 0.20 7.49 7.50 7.83 0.21 3.19 18.13 17.22 16.11 -1.79 3.17 19.44 19.17 18.47 -1.31
Eisosomes -2.83 -1.47 4.54 6.19 6.00 4.40 1.10 -2.96 -4.22 -3.29 2.52 -1.26 2.97 0.66 3.37
Endoplasmic Reticulum -4.79 -12.51 -15.84 -5.26 3.11 -2.82 -14.61 -7.01 -6.80 -3.56 -4.82 -18.89 -19.45 -17.87 -3.42
Endosome -2.88 0.45 0.26 3.39 -0.20 -2.78 8.57 2.45 4.16 -2.35 -3.33 7.33 1.22 3.52 -2.35
Golgi -2.71 2.15 -0.44 0.99 -0.93 -3.02 11.57 9.79 4.61 -1.49 -3.25 10.84 -0.28 1.43 -1.24
Lipid Particles 0.85 4.91 5.27 7.39 4.17 -2.94 10.61 8.18 9.88 -3.24 -2.15 11.16 11.52 12.99 1.55
Mitochondria -1.58 3.51 2.16 1.94 -2.25 -7.70 4.27 2.72 9.52 -1.03 -7.76 4.44 0.28 8.29 -6.12
Mitotic Spindle -0.04 0.78 -3.74 -4.96 -6.22 -4.88 0.83 1.39 5.86 0.81 -4.76 1.04 -5.86 -3.07 -6.37
None -0.47 5.80 1.95 1.67 -4.11 10.39 14.75 7.60 1.70 -0.93 10.09 15.51 10.92 2.69 -3.38
Nuclear Periphery 0.07 1.79 -3.15 -3.10 -6.09 -7.97 0.44 -0.18 5.13 -0.36 -7.81 0.77 -6.09 0.43 -6.56
Nuclear Periphery Foci -0.15 2.36 2.41 2.80 -0.01 2.57 6.35 2.47 0.92 -1.24 2.38 6.44 4.16 2.10 -2.06
Nucleolus 1.82 3.60 4.74 4.48 2.38 7.17 18.33 16.03 12.02 0.03 7.33 17.92 17.31 13.77 -0.70
Nucleus 0.28 -8.07 -6.19 -6.84 4.14 -11.84 -34.54 -11.30 -6.12 2.86 -11.15 -34.59 -20.37 -10.96 10.25
Peroxisomes -3.31 4.19 4.81 5.33 4.06 -5.19 10.29 8.97 10.73 -2.49 -5.85 10.12 10.53 11.71 0.96
Vacuole -3.18 -6.87 -8.95 -2.57 -0.24 -3.46 -6.33 -3.71 -3.58 -2.83 -4.60 -8.81 -9.98 -8.81 -3.75
Vacuole Periphery -3.59 -0.27 -2.00 -1.19 -1.97 -4.37 4.77 -0.10 3.18 -2.05 -4.86 3.55 -2.08 -1.40 -2.27
Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
Localization
Cell Percentages nucleus (77%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Nse4

Nse4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nse4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available