Standard name
Description Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0.06 0.08 0.07 0.05 0 0.06 0.07 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.34 0.41 0.18 0.32 0.19 0.18 0.13 0.14 0.16 0.11 0.09 0.09 0.8 0.88 0.89 0.2 0.21 0.22 0.09 0.09 0 0.13 0.13 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0.07 0 0.05 0.2 0.2 0.12 0.08 0.08 0.14 0.06 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.49 0.52 0.61 0.65 0.86 0.87 0.86 0.84 0.92 0.92 0.94 0 0 0 0.21 0.17 0.12 0.61 0.68 0.65 0.66 0.54 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.16 0.11 0.09 0 0 0 0 0 0 0 0.06 0 0 0.08 0.1 0.19 0 0 0.08 0 0 0
SpindlePole 0 0 0.16 0.06 0.09 0.06 0.06 0.07 0.05 0 0 0.05 0 0 0 0.16 0.19 0.21 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0 0.16 0.14 0.19 0.05 0.07 0 0 0.08 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 1 0 0 0 0 0 0 0 0 0 1 2 1 2 2 0 3 4 5 0 4 8
Bud 2 3 6 7 8 15 29 19 11 11 13 18 2 1 0 10 15 1 1 1 0 0 0 2
Bud Neck 1 0 1 0 2 0 0 1 0 0 0 0 1 2 1 0 3 3 0 0 0 0 2 4
Bud Site 0 0 1 1 0 2 10 6 3 5 6 8 0 0 0 1 1 1
Cell Periphery 0 2 1 9 2 2 1 0 0 0 4 1 0 3 2 10 10 7 0 0 0 0 0 0
Cytoplasm 40 41 35 52 37 45 45 39 33 28 19 23 83 130 149 36 34 29 10 12 3 7 18 25
Endoplasmic Reticulum 2 2 5 3 1 1 0 0 0 1 1 1 2 0 2 9 5 6 4 1 2 0 1 1
Endosome 0 2 15 6 14 0 0 6 5 0 0 0 7 6 9 37 32 16 9 10 39 3 8 6
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 4 0 2 1 0 5 0 0 1
Mitochondria 95 49 102 99 125 215 302 238 171 227 192 231 4 0 1 38 27 16 73 95 181 37 76 108
Nucleus 0 0 1 0 0 0 7 3 5 1 0 3 1 1 0 7 5 4 0 0 0 1 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 4 1 2 0 0 0 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 3 0 2 1 0 2 4 1 0 0 0 0 2 0 0 0 0 0 1 0
Peroxisomes 0 0 31 18 17 4 7 5 4 1 1 1 6 0 2 14 16 25 2 1 21 0 1 1
SpindlePole 0 0 32 10 17 15 21 19 11 11 7 13 1 4 3 29 31 27 2 0 4 1 5 7
Vac/Vac Membrane 2 4 11 2 8 10 12 8 12 5 5 6 3 4 5 29 23 24 5 9 3 1 11 13
Unique Cell Count 119 101 198 162 192 251 346 276 204 246 208 247 104 147 167 181 161 129 121 141 278 58 141 191
Labelled Cell Count 142 108 243 207 234 309 436 345 256 296 253 308 111 155 175 227 206 161 121 141 278 58 141 191


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.2 4.2 4.7 4.1 3.5 3.3 3.4 4.0 3.1 3.3 3.0 5.8 5.6 5.5 6.0 5.8 5.8 5.3 6.1 6.2
Std Deviation (1e-4) 0.8 0.5 1.3 3.8 1.8 1.6 1.5 1.7 3.2 1.4 2.6 1.3 1.9 1.4 1.6 1.5 1.5 1.6 0.9 2.0 1.5
Intensity Change (Log2) 0.17 -0.03 -0.27 -0.34 -0.3 -0.06 -0.44 -0.33 -0.48 0.47 0.4 0.39 0.52 0.46 0.47 0.32 0.54 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.3 2.8 3.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qri7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available