Standard name | |
---|---|
Human Ortholog | |
Description | Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 5 | 4 | 8 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 3 |
Bud | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 3 | 4 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 4 | 13 | 18 | 26 | 29 | 23 | 18 | 21 | 31 | 31 | 24 | 14 | 7 | 18 | 28 | 12 | 22 | 0 | 0 | 0 | 1 | 3 | 0 |
Cytoplasm | 194 | 188 | 259 | 232 | 397 | 341 | 365 | 286 | 265 | 304 | 225 | 179 | 222 | 280 | 315 | 328 | 202 | 385 | 104 | 152 | 227 | 249 | 293 | 303 |
Endoplasmic Reticulum | 32 | 36 | 68 | 35 | 75 | 61 | 51 | 66 | 44 | 68 | 38 | 49 | 16 | 8 | 10 | 5 | 2 | 7 | 120 | 47 | 95 | 54 | 58 | 54 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 4 | 5 |
Golgi | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 3 | 0 | 6 | 9 |
Mitochondria | 1 | 1 | 0 | 0 | 0 | 2 | 9 | 4 | 15 | 12 | 23 | 8 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 3 | 1 |
Nucleus | 2 | 0 | 1 | 1 | 0 | 1 | 4 | 6 | 1 | 10 | 4 | 3 | 5 | 3 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 1 | 0 | 6 | 6 | 25 | 27 | 85 | 76 | 48 | 62 | 25 | 20 | 0 | 1 | 0 | 37 | 7 | 23 | 2 | 2 | 6 | 2 | 8 | 4 |
Unique Cell Count | 223 | 215 | 316 | 270 | 478 | 427 | 503 | 431 | 375 | 451 | 321 | 267 | 236 | 284 | 322 | 374 | 213 | 417 | 239 | 207 | 348 | 318 | 396 | 398 |
Labelled Cell Count | 236 | 230 | 348 | 294 | 523 | 464 | 545 | 463 | 401 | 498 | 356 | 292 | 258 | 300 | 345 | 405 | 224 | 442 | 239 | 207 | 348 | 318 | 396 | 398 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.1 | 23.7 | 18.5 | 18.0 | 17.6 | 14.9 | 13.8 | 14.5 | 13.2 | 13.2 | 11.8 | 12.5 | 23.2 | 22.1 | 22.9 | 29.8 | 27.5 | 27.7 | 21.3 | 21.6 | 22.7 |
Std Deviation (1e-4) | 2.9 | 4.4 | 3.4 | 2.5 | 2.7 | 2.5 | 2.8 | 3.0 | 3.9 | 2.7 | 2.6 | 2.7 | 4.1 | 3.6 | 3.8 | 8.0 | 6.7 | 7.3 | 4.3 | 4.8 | 4.8 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.07 | -0.3 | -0.42 | -0.35 | -0.48 | -0.48 | -0.64 | -0.56 | 0.33 | 0.26 | 0.31 | 0.69 | 0.58 | 0.59 | 0.21 | 0.23 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.4 | 0.8 | 1.6 | 0.3 | 0 | 0.9 | 1.6 | 2.8 | 2.4 | 1.0 | -1.1 | 0.9 | 1.9 | 0.8 | 0.7 |
Cytoplasm | 1.3 | 0.4 | -0.7 | -3.1 | -4.7 | -3.5 | -4.5 | -3.5 | -4.2 | 4.2 | 6.7 | 6.7 | 2.1 | 4.3 | 4.3 |
Endoplasmic Reticulum | -2.7 | -2.1 | -2.6 | -4.5 | -2.2 | -3.5 | -2.3 | -3.3 | -1.0 | -4.8 | -6.9 | -7.1 | -8.6 | -6.9 | -8.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | 2.4 | 2.9 | 6.6 | 6.8 | 5.3 | 5.7 | 3.5 | 3.3 | 0 | 0 | 0 | 4.3 | 1.0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.1142 | 20.7924 | 19.4869 | 17.4423 | 16.1794 | 19.2114 | 20.5026 | 22.3587 | 20.4336 | 19.4852 | 20.2267 | 20.0433 | 18.5996 | 21.7244 | 19.8663 | 17.4778 | 18.0363 | 20.2306 |
Actin | 0.0145 | 0.0009 | 0.0025 | 0.0047 | 0.0062 | 0.0012 | 0.0002 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0135 | 0.0012 | 0.0046 | 0.021 | 0.0041 | 0.0036 |
Bud | 0.0007 | 0.0007 | 0.0005 | 0.0011 | 0.0006 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0011 | 0.0003 | 0.0001 | 0.0012 | 0 | 0.0001 |
Bud Neck | 0.0015 | 0.0005 | 0.001 | 0.0016 | 0.0043 | 0.0029 | 0.0002 | 0.0004 | 0.0004 | 0.0013 | 0.0018 | 0.0017 | 0.0008 | 0.0003 | 0.0007 | 0.0051 | 0.0005 | 0.0016 |
Bud Periphery | 0.0011 | 0.0001 | 0.0006 | 0.0011 | 0.0011 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 |
Bud Site | 0.0025 | 0.0006 | 0.0052 | 0.0015 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0019 | 0.0014 | 0.0019 | 0.0054 | 0 | 0.0001 |
Cell Periphery | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.7559 | 0.9271 | 0.8664 | 0.8077 | 0.8119 | 0.8687 | 0.8321 | 0.8492 | 0.826 | 0.9068 | 0.9019 | 0.8563 | 0.7538 | 0.9005 | 0.7863 | 0.7629 | 0.7927 | 0.8382 |
Cytoplasmic Foci | 0.0115 | 0.0018 | 0.0025 | 0.0026 | 0.0021 | 0.0041 | 0.0006 | 0.001 | 0.0009 | 0.0009 | 0.0005 | 0.0006 | 0.0099 | 0.0094 | 0.0049 | 0.0114 | 0.0009 | 0.0009 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0987 | 0.0483 | 0.0815 | 0.1 | 0.0481 | 0.0602 | 0.1296 | 0.1265 | 0.1395 | 0.0711 | 0.0686 | 0.114 | 0.1094 | 0.0558 | 0.1372 | 0.0718 | 0.0927 | 0.12 |
Endosome | 0.0266 | 0.0009 | 0.0058 | 0.0183 | 0.0216 | 0.0128 | 0.0013 | 0.0008 | 0.0007 | 0.0005 | 0.0003 | 0.0006 | 0.0192 | 0.001 | 0.0051 | 0.0197 | 0.0094 | 0.0031 |
Golgi | 0.0065 | 0.0001 | 0.0037 | 0.0044 | 0.0039 | 0.0033 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0025 | 0.0002 | 0.0019 | 0.0055 | 0.0033 | 0.0004 |
Lipid Particles | 0.003 | 0.0017 | 0.0005 | 0.001 | 0.0022 | 0.0025 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0036 | 0.0018 | 0.0027 | 0.0154 | 0.0058 | 0.0002 |
Mitochondria | 0.004 | 0.0001 | 0.0034 | 0.0039 | 0.014 | 0.001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0035 | 0.0001 | 0.0017 | 0.0153 | 0.0034 | 0.0004 |
None | 0.0031 | 0.0006 | 0.0012 | 0.0007 | 0.0013 | 0.0007 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0005 | 0.0033 | 0.0063 | 0.0134 | 0.0019 | 0.0009 | 0.0004 |
Nuclear Periphery | 0.0266 | 0.0073 | 0.0106 | 0.0234 | 0.036 | 0.0163 | 0.0178 | 0.0069 | 0.0147 | 0.0054 | 0.0082 | 0.0086 | 0.0407 | 0.0112 | 0.0221 | 0.0234 | 0.0522 | 0.0137 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0 |
Nucleus | 0.0273 | 0.0054 | 0.0099 | 0.02 | 0.0217 | 0.0198 | 0.0151 | 0.0109 | 0.0144 | 0.0123 | 0.0172 | 0.0155 | 0.0208 | 0.0078 | 0.0121 | 0.0165 | 0.0217 | 0.0151 |
Peroxisomes | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0003 | 0.009 | 0.0001 | 0 |
Punctate Nuclear | 0.0047 | 0.0005 | 0.0008 | 0.0011 | 0.0008 | 0.0012 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0004 | 0.0004 | 0.0046 | 0.0006 | 0.0025 | 0.0031 | 0.001 | 0.0005 |
Vacuole | 0.0086 | 0.0026 | 0.0025 | 0.0049 | 0.0183 | 0.0037 | 0.0016 | 0.0014 | 0.001 | 0.0004 | 0.0003 | 0.0011 | 0.0071 | 0.0014 | 0.0013 | 0.006 | 0.0078 | 0.0013 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0004 | 0.0014 | 0.0045 | 0.0009 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0018 | 0.0001 | 0.0004 | 0.0028 | 0.0028 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 279.7363 | 236.1746 | 187.6224 | 239.1714 | 222.3158 | 190.5296 | 240.9029 | 228.1677 | 229.4605 | 253.7634 |
Translational Efficiency | 1.3844 | 1.3806 | 1.5058 | 1.2737 | 1.495 | 2.0161 | 1.3302 | 1.3198 | 1.3715 | 1.3852 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2044 | 968 | 2161 | 117 | 2205 | 907 | 254 | 172 | 4249 | 1875 | 2415 | 289 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1480.21 | 1792.12 | 1836.93 | 1980.76 | 1451.27 | 1562.77 | 1984.96 | 1770.41 | 1465.19 | 1681.18 | 1852.50 | 1855.57 |
Standard Deviation | 228.87 | 291.45 | 369.68 | 322.37 | 232.36 | 266.84 | 353.14 | 252.99 | 231.14 | 302.38 | 370.77 | 301.37 |
Intensity Change Log 2 | — | 0.275865 | 0.311495 | 0.420252 | — | 0.106790 | 0.451794 | 0.286768 | — | 0.194636 | 0.382657 | 0.355711 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000688 | 0.001302 | 0.001396 | 0.004179 | 0.000578 | 0.001189 | 0.001590 | 0.001590 | 0.000631 | 0.001247 | 0.001416 | 0.002638 |
Bud Neck | 0.001134 | 0.003053 | 0.004128 | 0.011066 | 0.001564 | 0.001631 | 0.013191 | 0.007841 | 0.001357 | 0.002365 | 0.005081 | 0.009147 |
Bud Site | 0.005852 | 0.014853 | 0.016985 | 0.028478 | 0.005936 | 0.009112 | 0.018818 | 0.019241 | 0.005896 | 0.012076 | 0.017178 | 0.022981 |
Cell Periphery | 0.000859 | 0.003773 | 0.006110 | 0.018081 | 0.000896 | 0.001770 | 0.008823 | 0.015957 | 0.000878 | 0.002804 | 0.006396 | 0.016817 |
Cytoplasm | 0.414825* | 0.438085* | 0.626921* | 0.134185* | 0.446454* | 0.405949* | 0.178115* | 0.202750* | 0.431239* | 0.422540* | 0.579718* | 0.174992* |
Cytoplasmic Foci | 0.030910 | 0.023218 | 0.012587 | 0.008899 | 0.032395 | 0.025832 | 0.018154 | 0.011160 | 0.031681 | 0.024482 | 0.013173 | 0.010245 |
Eisosomes | 0.000043 | 0.000065 | 0.000081 | 0.000118 | 0.000039 | 0.000047 | 0.000078 | 0.000067 | 0.000041 | 0.000056 | 0.000081 | 0.000087 |
Endoplasmic Reticulum | 0.437106* | 0.342948* | 0.120426* | 0.286927* | 0.357075* | 0.405854* | 0.250408* | 0.367938* | 0.395574* | 0.373378* | 0.134097* | 0.335141* |
Endosome | 0.008164 | 0.010626 | 0.005793 | 0.025569 | 0.009271 | 0.012006 | 0.028095 | 0.020796 | 0.008738 | 0.011294 | 0.008138 | 0.022728 |
Golgi | 0.007128 | 0.011421 | 0.006028 | 0.008674 | 0.005276 | 0.009791 | 0.004469 | 0.012722 | 0.006167 | 0.010633 | 0.005864 | 0.011083 |
Lipid Particles | 0.016807 | 0.047100 | 0.065888 | 0.163220* | 0.015095 | 0.032525 | 0.105932* | 0.111163* | 0.015919 | 0.040049 | 0.070099 | 0.132238* |
Mitochondria | 0.000962 | 0.000483 | 0.000900 | 0.000029 | 0.000979 | 0.000388 | 0.000097 | 0.000056 | 0.000971 | 0.000437 | 0.000815 | 0.000045 |
Mitotic Spindle | 0.001941 | 0.006838 | 0.001051 | 0.001275 | 0.000797 | 0.004683 | 0.002789 | 0.005372 | 0.001348 | 0.005795 | 0.001234 | 0.003713 |
None | 0.004476 | 0.002823 | 0.005465 | 0.000043 | 0.005294 | 0.001950 | 0.000501 | 0.000352 | 0.004900 | 0.002401 | 0.004942 | 0.000227 |
Nuclear Periphery | 0.001393 | 0.000802 | 0.000393 | 0.001908 | 0.005329 | 0.000592 | 0.003716 | 0.001241 | 0.003435 | 0.000700 | 0.000742 | 0.001511 |
Nuclear Periphery Foci | 0.029321 | 0.053168 | 0.031099 | 0.124865 | 0.021335 | 0.053532 | 0.130517 | 0.127428 | 0.025177 | 0.053344 | 0.041555 | 0.126391 |
Nucleolus | 0.000157 | 0.000093 | 0.000190 | 0.000013 | 0.000241 | 0.000073 | 0.000077 | 0.000026 | 0.000201 | 0.000084 | 0.000178 | 0.000021 |
Nucleus | 0.012901 | 0.009548 | 0.016235 | 0.004536 | 0.021373 | 0.007346 | 0.012818 | 0.004171 | 0.017297 | 0.008483 | 0.015876 | 0.004319 |
Peroxisomes | 0.000228 | 0.000216 | 0.000354 | 0.000275 | 0.000238 | 0.000150 | 0.000451 | 0.000127 | 0.000234 | 0.000184 | 0.000364 | 0.000187 |
Vacuole | 0.023534 | 0.028373 | 0.076633 | 0.176772 | 0.067581 | 0.023869 | 0.220598* | 0.088218 | 0.046392 | 0.026194 | 0.091775 | 0.124069 |
Vacuole Periphery | 0.001570 | 0.001211 | 0.001338 | 0.000889 | 0.002254 | 0.001712 | 0.000763 | 0.001783 | 0.001925 | 0.001453 | 0.001278 | 0.001421 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.06 | -4.91 | -2.37 | -1.97 | -1.87 | -2.82 | -3.32 | -1.96 | -0.75 | 0.66 | -4.19 | -6.23 | -2.95 | -2.07 | -1.66 |
Bud Neck | -6.30 | -12.48 | -8.97 | -6.99 | -6.04 | -0.41 | -8.89 | -5.88 | -5.78 | 3.18 | -5.41 | -14.28 | -9.96 | -8.48 | -4.82 |
Bud Site | -5.11 | -8.82 | -2.56 | -1.52 | -1.24 | -2.15 | -3.77 | -2.18 | -1.62 | 0.33 | -5.33 | -9.92 | -3.36 | -2.09 | -0.94 |
Cell Periphery | -7.31 | -10.38 | -5.89 | -4.77 | -3.94 | -5.50 | -7.25 | -4.82 | -4.53 | -2.28 | -8.72 | -11.80 | -7.21 | -6.26 | -4.55 |
Cytoplasm | -1.84 | -21.83 | 14.43 | 14.52 | 25.27 | 3.08 | 14.89 | 11.90 | 9.15 | -0.16 | 0.95 | -17.99 | 17.55 | 15.76 | 26.38 |
Cytoplasmic Foci | 3.58 | 10.94 | 9.66 | 5.70 | 1.42 | 2.75 | 3.71 | 8.26 | 5.08 | 2.86 | 4.48 | 13.72 | 12.07 | 7.25 | 1.62 |
Eisosomes | -2.42 | -5.34 | -3.64 | -2.58 | -2.03 | -1.24 | -5.23 | -1.82 | -1.30 | 0.79 | -2.73 | -6.68 | -3.85 | -2.58 | -0.86 |
Endoplasmic Reticulum | 7.37 | 36.08* | 6.31 | 2.78 | -5.17 | -3.53 | 7.76 | -0.06 | 1.64 | -4.72 | 2.46 | 39.17 | 3.87 | 2.57 | -9.70 |
Endosome | -3.57 | 5.19 | -7.33 | -6.08 | -8.46 | -2.64 | -10.09 | -6.80 | -4.62 | 2.44 | -4.16 | 1.82 | -10.01 | -7.64 | -10.55 |
Golgi | -4.06 | 2.05 | -0.88 | 2.39 | -2.00 | -3.96 | 2.07 | -4.06 | -1.29 | -4.61 | -5.78 | 0.92 | -3.93 | -0.06 | -4.22 |
Lipid Particles | -8.00 | -16.17 | -7.92 | -6.17 | -5.15 | -5.41 | -8.96 | -6.89 | -5.50 | -0.39 | -9.64 | -19.02 | -10.34 | -8.01 | -5.26 |
Mitochondria | 3.58 | 0.52 | 7.70 | 7.31 | 5.42 | 3.73 | 7.31 | 7.88 | 3.13 | 2.37 | 5.18 | 1.16 | 10.99 | 6.43 | 5.38 |
Mitotic Spindle | -2.79 | 0.90 | 0.35 | 2.97 | -0.63 | -2.20 | -3.16 | -1.16 | -0.30 | -0.65 | -3.58 | -0.63 | -1.09 | 0.47 | -1.00 |
None | 1.83 | -0.61 | 5.66 | 6.16 | 5.55 | 3.33 | 5.69 | 6.00 | 2.55 | 0.94 | 3.71 | 0.16 | 8.17 | 5.61 | 5.36 |
Nuclear Periphery | 3.02 | 5.51 | -1.75 | -4.57 | -6.62 | 7.73 | 2.27 | 6.40 | -3.20 | 4.89 | 8.20 | 8.04 | 5.38 | -5.13 | -4.76 |
Nuclear Periphery Foci | -7.07 | -1.68 | -7.20 | -5.21 | -6.92 | -8.84 | -12.35 | -9.10 | -6.12 | -0.27 | -11.39 | -8.90 | -11.55 | -8.05 | -9.43 |
Nucleolus | 1.67 | -0.51 | 4.01 | 4.42 | 14.10 | 4.79 | 4.29 | 7.06 | 2.31 | 3.45 | 4.44 | 1.15 | 7.61 | 4.59 | 13.38 |
Nucleus | 3.21 | -3.88 | 5.47 | 3.00 | 7.78 | 12.10 | 1.37 | 14.35 | 3.11 | 6.44 | 11.38 | 0.14 | 14.51 | 4.65 | 11.91 |
Peroxisomes | 0.37 | -1.90 | -0.15 | -0.29 | 0.48 | 2.66 | -1.30 | 2.46 | 0.47 | 2.10 | 1.66 | -2.94 | 0.74 | 0.02 | 2.20 |
Vacuole | -2.34 | -21.00 | -10.88 | -10.49 | -7.14 | 12.03 | -11.59 | -2.10 | -6.74 | 8.37 | 9.12 | -14.82 | -9.19 | -11.56 | -3.76 |
Vacuole Periphery | 1.38 | 1.03 | 2.42 | 1.59 | 2.19 | 1.04 | 5.44 | 0.92 | -0.04 | -2.16 | 1.65 | 3.41 | 1.58 | 0.22 | -0.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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