Standard name | |
---|---|
Human Ortholog | |
Description | Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 8 | 6 | 3 | 1 | 0 | 0 | 0 | – | 2 | 4 | 2 | 3 | 0 | 2 | 7 | 8 | 13 | 17 | 16 | 18 | 18 | 29 | 21 |
Bud | 2 | 2 | 5 | 2 | 2 | 6 | 7 | 15 | – | 6 | 6 | 14 | 1 | 1 | 1 | 6 | 4 | 2 | 2 | 2 | 1 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 0 | 2 | 7 | 1 | 2 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 9 | 7 | 6 | 4 | 6 | 13 | 12 | 12 | – | 19 | 19 | 28 | 2 | 14 | 16 | 34 | 10 | 30 | 2 | 4 | 7 | 8 | 26 | 26 |
Cytoplasm | 160 | 103 | 112 | 62 | 76 | 114 | 124 | 202 | – | 128 | 72 | 122 | 32 | 47 | 159 | 377 | 190 | 137 | 36 | 48 | 23 | 10 | 20 | 23 |
Endoplasmic Reticulum | 125 | 120 | 69 | 43 | 35 | 54 | 48 | 24 | – | 71 | 41 | 26 | 56 | 46 | 54 | 22 | 23 | 10 | 269 | 181 | 202 | 96 | 159 | 143 |
Endosome | 3 | 0 | 13 | 3 | 12 | 3 | 2 | 8 | – | 1 | 0 | 1 | 3 | 1 | 1 | 5 | 0 | 2 | 7 | 5 | 3 | 4 | 1 | 3 |
Golgi | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 4 | 2 | 5 | 4 | 2 |
Mitochondria | 13 | 26 | 29 | 65 | 56 | 173 | 222 | 301 | – | 264 | 187 | 278 | 1 | 1 | 0 | 58 | 18 | 9 | 26 | 30 | 24 | 34 | 42 | 31 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | – | 2 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 1 | 1 | 1 | 0 | 1 | 2 | – | 8 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 42 | 7 | 17 | 6 | 39 | 12 | 30 | 38 | – | 20 | 3 | 17 | 9 | 3 | 3 | 20 | 2 | 5 | 8 | 10 | 8 | 6 | 12 | 17 |
Unique Cell Count | 315 | 248 | 230 | 157 | 193 | 298 | 353 | 480 | 398 | 269 | 371 | 97 | 99 | 216 | 437 | 232 | 185 | 384 | 317 | 301 | 191 | 308 | 283 | |
Labelled Cell Count | 368 | 275 | 263 | 189 | 231 | 377 | 449 | 605 | 523 | 336 | 497 | 108 | 114 | 237 | 536 | 268 | 216 | 384 | 317 | 301 | 191 | 308 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.4 | 5.3 | 5.2 | 5.5 | 4.5 | 4.2 | 4.6 | – | 4.0 | 4.1 | 4.0 | 7.0 | 6.9 | 7.2 | 5.2 | 5.3 | 6.5 | 6.6 | 6.9 | 7.7 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.5 | 1.3 | 1.3 | 1.2 | 1.0 | 1.3 | – | 0.8 | 0.7 | 0.9 | 0.8 | 0.9 | 1.2 | 1.4 | 0.8 | 1.2 | 1.2 | 1.1 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.06 | -0.23 | -0.32 | -0.21 | – | -0.39 | -0.37 | -0.4 | 0.42 | 0.38 | 0.44 | -0.02 | 0.02 | 0.3 | 0.33 | 0.4 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 2.1 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4.0 | 2.3 | 2.7 | 1.0 | 4.9 |
Cytoplasm | -1.8 | -1.9 | -2.4 | -3.3 | -1.7 | – | -4.1 | -5.1 | -3.9 | -2.6 | -0.2 | 5.4 | 10.4 | 7.5 | 5.2 |
Endoplasmic Reticulum | -0.6 | -2.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.7 | 2.9 | -1.2 | -8.9 | -5.4 | -6.3 |
Endosome | -1.8 | 0.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.1 | -3.4 | -3.7 | -2.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.5 | 4.2 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.3 | -3.4 | -5.4 | 0.2 | -1.7 | -2.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | 3.9 | -1.7 | 0.5 | 0.2 | – | -1.2 | -3.6 | -1.4 | 0.6 | -1.5 | -3.1 | -1.5 | -3.5 | -2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6161 | 5.3538 | 5.9047 | 5.1702 | 5.1926 | 5.493 | 8.0111 | 8.6145 | 8.8389 | 8.6352 | 7.9961 | 7.9556 | 7.3664 | 7.4897 | 8.0524 | 7.9051 | 7.5202 | 7.4164 |
Actin | 0.0012 | 0.006 | 0.01 | 0.0129 | 0.0022 | 0.0022 | 0.0028 | 0.0017 | 0.0015 | 0.0015 | 0.0018 | 0.0016 | 0.0033 | 0.002 | 0.0009 | 0.0009 | 0.0015 | 0.0022 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 |
Bud Neck | 0.0002 | 0.0008 | 0.0018 | 0.0185 | 0.0017 | 0.0026 | 0.0003 | 0.0007 | 0.0011 | 0.0027 | 0.003 | 0.0023 | 0.0003 | 0.0003 | 0.0008 | 0.0003 | 0.0004 | 0.0018 |
Bud Periphery | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 |
Bud Site | 0.0005 | 0.0088 | 0.0013 | 0.0012 | 0.0003 | 0.001 | 0.0003 | 0.0048 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0011 | 0.0005 | 0 | 0 | 0.0002 |
Cell Periphery | 0.0032 | 0.0045 | 0.0027 | 0.0015 | 0.0015 | 0.0022 | 0.0072 | 0.0052 | 0.009 | 0.0126 | 0.0043 | 0.0054 | 0.0021 | 0.0039 | 0.0044 | 0.0032 | 0.0009 | 0.0057 |
Cytoplasm | 0.0802 | 0.2088 | 0.1323 | 0.0786 | 0.0762 | 0.11 | 0.0565 | 0.0781 | 0.0544 | 0.048 | 0.1085 | 0.0766 | 0.0698 | 0.1086 | 0.0443 | 0.0252 | 0.1019 | 0.0881 |
Cytoplasmic Foci | 0.0035 | 0.0095 | 0.007 | 0.0156 | 0.0039 | 0.0028 | 0.0035 | 0.0038 | 0.003 | 0.0038 | 0.005 | 0.0032 | 0.0026 | 0.0042 | 0.0021 | 0.001 | 0.0029 | 0.0053 |
Eisosomes | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.8656 | 0.6858 | 0.7944 | 0.8172 | 0.8733 | 0.8482 | 0.8894 | 0.8421 | 0.8896 | 0.8874 | 0.8264 | 0.8713 | 0.8884 | 0.8244 | 0.9196 | 0.9501 | 0.8677 | 0.8601 |
Endosome | 0.0086 | 0.0242 | 0.0152 | 0.0159 | 0.0154 | 0.0089 | 0.0065 | 0.013 | 0.0081 | 0.0077 | 0.0136 | 0.0099 | 0.0084 | 0.0195 | 0.0056 | 0.0071 | 0.0056 | 0.0091 |
Golgi | 0.0018 | 0.0067 | 0.0081 | 0.0088 | 0.0073 | 0.0012 | 0.0024 | 0.007 | 0.004 | 0.0028 | 0.0048 | 0.0019 | 0.0024 | 0.0045 | 0.0021 | 0.0018 | 0.0027 | 0.0027 |
Lipid Particles | 0.0089 | 0.0088 | 0.0065 | 0.0047 | 0.0026 | 0.0036 | 0.0096 | 0.0122 | 0.0088 | 0.0118 | 0.0095 | 0.0083 | 0.0064 | 0.0156 | 0.0083 | 0.0015 | 0.0052 | 0.0106 |
Mitochondria | 0.0003 | 0.0042 | 0.0018 | 0.0009 | 0.0034 | 0.0007 | 0.0002 | 0.008 | 0.0007 | 0.0014 | 0.0039 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0006 | 0.0002 | 0.0004 |
None | 0.0087 | 0.0029 | 0.002 | 0.0036 | 0.0009 | 0.0026 | 0.0115 | 0.0054 | 0.0094 | 0.0075 | 0.007 | 0.008 | 0.0021 | 0.0023 | 0.0041 | 0.0002 | 0.0059 | 0.0041 |
Nuclear Periphery | 0.0106 | 0.0141 | 0.0099 | 0.0091 | 0.0048 | 0.0072 | 0.0047 | 0.0069 | 0.0046 | 0.0056 | 0.0061 | 0.0056 | 0.0088 | 0.0074 | 0.004 | 0.0048 | 0.0031 | 0.0037 |
Nucleolus | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Nucleus | 0.0018 | 0.0043 | 0.0014 | 0.0039 | 0.0016 | 0.0021 | 0.0006 | 0.0015 | 0.0007 | 0.0007 | 0.0011 | 0.001 | 0.0012 | 0.0006 | 0.0003 | 0.0005 | 0.0004 | 0.0009 |
Peroxisomes | 0.0001 | 0.0002 | 0.0002 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0004 | 0.0008 | 0.0004 | 0.0009 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0011 |
Vacuole | 0.0036 | 0.0078 | 0.0036 | 0.004 | 0.0034 | 0.0031 | 0.0029 | 0.0073 | 0.0033 | 0.0046 | 0.0029 | 0.0028 | 0.0021 | 0.0039 | 0.0021 | 0.002 | 0.0012 | 0.0028 |
Vacuole Periphery | 0.0004 | 0.0008 | 0.0008 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0006 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 199.4132 | 184.4933 | 120.7959 | 140.759 | 209.5783 | 164.4753 | 182.1721 | 153.3324 | 165.0879 | 223.5581 |
Translational Efficiency | 0.9533 | 0.923 | 0.8597 | 0.7472 | 0.788 | 1.1005 | 0.7661 | 0.6312 | 0.6693 | 0.6826 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
415 | 1429 | 1756 | 1565 | 2201 | 1673 | 370 | 1311 | 2616 | 3102 | 2126 | 2876 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 938.41 | 815.27 | 979.62 | 989.42 | 775.28 | 801.57 | 989.75 | 949.54 | 801.16 | 807.88 | 981.38 | 971.24 |
Standard Deviation | 135.80 | 118.73 | 119.66 | 139.47 | 96.46 | 93.74 | 161.79 | 139.81 | 119.61 | 106.20 | 128.05 | 141.03 |
Intensity Change Log 2 | — | -0.202940 | 0.062004 | 0.076365 | — | 0.048111 | 0.352347 | 0.292511 | — | -0.083929 | 0.200628 | 0.178177 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.004589 | 0.000914 | 0.000364 | 0.004886 | 0.000279 | 0.000976 | 0.003515 | 0.009670 | 0.000963 | 0.000947 | 0.000912 | 0.007067 |
Bud Neck | 0.006042 | 0.006910 | 0.002762 | 0.012425 | 0.002445 | 0.009672 | 0.002321 | 0.012654 | 0.003015 | 0.008400 | 0.002685 | 0.012529 |
Bud Site | 0.031962 | 0.007739 | 0.004665 | 0.029535 | 0.001851 | 0.006245 | 0.006342 | 0.032170 | 0.006628 | 0.006933 | 0.004957 | 0.030736 |
Cell Periphery | 0.001886 | 0.000669 | 0.000347 | 0.000890 | 0.000868 | 0.000712 | 0.000704 | 0.002079 | 0.001029 | 0.000693 | 0.000409 | 0.001432 |
Cytoplasm | 0.170609* | 0.297456* | 0.229828* | 0.234309* | 0.262398* | 0.254226* | 0.136148* | 0.218008* | 0.247837* | 0.274141* | 0.213524* | 0.226879* |
Cytoplasmic Foci | 0.069813 | 0.166269 | 0.017414 | 0.044280 | 0.058365 | 0.131137 | 0.029365 | 0.056748 | 0.060181 | 0.147321 | 0.019494 | 0.049964 |
Eisosomes | 0.000958 | 0.000718 | 0.000057 | 0.000403 | 0.000935 | 0.001301 | 0.000214 | 0.001590 | 0.000939 | 0.001032 | 0.000084 | 0.000944 |
Endoplasmic Reticulum | 0.343579* | 0.231869* | 0.341208* | 0.240599* | 0.454791* | 0.306878* | 0.468782* | 0.368207* | 0.437149* | 0.272324* | 0.363410* | 0.298768* |
Endosome | 0.022642 | 0.021441 | 0.010642 | 0.019562 | 0.006324 | 0.017770 | 0.014506 | 0.015222 | 0.008913 | 0.019461 | 0.011315 | 0.017584 |
Golgi | 0.011522 | 0.022020 | 0.000414 | 0.003728 | 0.024003 | 0.032140 | 0.001895 | 0.006504 | 0.022023 | 0.027478 | 0.000672 | 0.004994 |
Lipid Particles | 0.041922 | 0.092242 | 0.015548 | 0.038853 | 0.102805* | 0.113573* | 0.031472 | 0.053062 | 0.093147 | 0.103746* | 0.018319 | 0.045330 |
Mitochondria | 0.001681 | 0.010273 | 0.000782 | 0.002507 | 0.010806 | 0.020357 | 0.000600 | 0.003610 | 0.009358 | 0.015711 | 0.000750 | 0.003010 |
Mitotic Spindle | 0.006721 | 0.002910 | 0.001785 | 0.021014 | 0.000202 | 0.001291 | 0.001158 | 0.003265 | 0.001236 | 0.002037 | 0.001676 | 0.012923 |
None | 0.000272 | 0.006185 | 0.001769 | 0.001329 | 0.007677 | 0.002522 | 0.001120 | 0.000854 | 0.006502 | 0.004209 | 0.001656 | 0.001112 |
Nuclear Periphery | 0.008784 | 0.006343 | 0.102483* | 0.018784 | 0.004099 | 0.003581 | 0.043448 | 0.004486 | 0.004842 | 0.004853 | 0.092209 | 0.012267 |
Nuclear Periphery Foci | 0.037427 | 0.009749 | 0.106829 | 0.051507 | 0.004897 | 0.003744 | 0.114614 | 0.042724 | 0.010057 | 0.006510 | 0.108184 | 0.047503 |
Nucleolus | 0.000222 | 0.001163 | 0.000312 | 0.000594 | 0.000277 | 0.000706 | 0.000874 | 0.000304 | 0.000268 | 0.000916 | 0.000410 | 0.000462 |
Nucleus | 0.040914 | 0.054187 | 0.116521 | 0.093639 | 0.040940 | 0.048655 | 0.074967 | 0.065604 | 0.040936 | 0.051203 | 0.109289 | 0.080860 |
Peroxisomes | 0.002083 | 0.005768 | 0.000298 | 0.002831 | 0.004317 | 0.009438 | 0.001409 | 0.004018 | 0.003963 | 0.007748 | 0.000492 | 0.003372 |
Vacuole | 0.194288 | 0.051795 | 0.045481 | 0.176046 | 0.010430 | 0.031888 | 0.066048 | 0.098075 | 0.039597 | 0.041058 | 0.049060 | 0.140503 |
Vacuole Periphery | 0.002086 | 0.003379 | 0.000489 | 0.002279 | 0.001291 | 0.003188 | 0.000498 | 0.001144 | 0.001417 | 0.003276 | 0.000491 | 0.001762 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.22 | 3.68 | 0.12 | -4.99 | -5.77 | -5.23 | -2.31 | -7.72 | -7.11 | -3.23 | 0.09 | 0.03 | -8.38 | -8.65 | -8.15 |
Bud Neck | -0.73 | 2.64 | -5.48 | -5.46 | -10.28 | -6.44 | -0.30 | -8.09 | -1.95 | -8.06 | -7.11 | 0.02 | -12.28 | -4.74 | -12.28 |
Bud Site | 4.24 | 4.73 | 0.44 | -8.21 | -9.30 | -4.89 | -3.12 | -10.40 | -8.58 | -7.78 | -0.28 | 1.09 | -11.33 | -11.95 | -12.42 |
Cell Periphery | 2.17 | 2.76 | 1.86 | -1.89 | -6.57 | 1.81 | 1.17 | -4.21 | -4.86 | -4.60 | 3.00 | 5.61 | -2.19 | -5.17 | -7.30 |
Cytoplasm | -9.94 | -4.66 | -4.80 | 7.38 | -0.31 | 1.17 | 11.42 | 5.40 | 4.22 | -6.89 | -4.32 | 5.12 | 3.51 | 7.89 | -1.83 |
Cytoplasmic Foci | -14.10 | 9.80 | 4.44 | 24.52 | -12.06 | -16.68 | 6.84 | 0.17 | 15.74 | -6.21 | -24.59 | 17.70 | 3.58 | 28.64 | -15.65 |
Eisosomes | 0.62 | 2.88 | 1.80 | 3.57 | -7.85 | -3.12 | 7.78 | -2.93 | -1.14 | -6.56 | -1.00 | 10.74 | 0.20 | 1.12 | -8.88 |
Endoplasmic Reticulum | 3.62 | -1.52 | 5.35 | 3.14 | 13.78 | 13.55 | 0.07 | 8.96 | -3.05 | 5.30 | 17.69 | 7.93 | 17.95 | 0.83 | 10.15 |
Endosome | -0.18 | 4.83 | 1.26 | 2.26 | -6.70 | -11.14 | -6.98 | -8.71 | 2.12 | -0.01 | -12.97 | -4.53 | -10.14 | 2.79 | -6.16 |
Golgi | -3.95 | 3.60 | 2.58 | 12.89 | -4.20 | -4.52 | 19.48 | 15.22 | 16.47 | -5.40 | -4.03 | 21.79 | 16.49 | 21.05 | -8.45 |
Lipid Particles | -9.73 | 5.68 | 0.92 | 15.13 | -10.60 | -2.26 | 15.72 | 12.44 | 14.12 | -5.09 | -2.93 | 25.69 | 15.66 | 21.10 | -14.48 |
Mitochondria | -7.70 | 1.42 | -1.38 | 7.58 | -5.74 | -5.52 | 13.19 | 6.91 | 9.84 | -3.70 | -5.47 | 13.11 | 8.74 | 12.52 | -5.46 |
Mitotic Spindle | 1.60 | 2.10 | -4.35 | -6.84 | -7.35 | -2.40 | -1.13 | -5.40 | -2.87 | -2.19 | -1.51 | -0.87 | -8.07 | -7.41 | -7.44 |
None | -4.82 | -3.56 | -3.43 | 3.98 | 1.07 | 5.34 | 6.66 | 7.51 | 4.50 | 0.88 | 2.38 | 5.85 | 7.03 | 5.16 | 1.63 |
Nuclear Periphery | 0.77 | -22.44 | -3.84 | -6.10 | 21.09 | 0.83 | -7.91 | -0.52 | -1.13 | 7.81 | -0.21 | -26.58 | -7.02 | -6.49 | 23.96 |
Nuclear Periphery Foci | 6.83 | -13.60 | -2.71 | -15.69 | 13.84 | 2.48 | -14.00 | -16.16 | -16.79 | 8.73 | 4.09 | -30.10 | -19.94 | -23.18 | 17.25 |
Nucleolus | -5.44 | -2.06 | -5.86 | 2.60 | -5.01 | -3.43 | -2.97 | -1.16 | 2.76 | 2.67 | -6.26 | -3.40 | -5.61 | 3.47 | -1.44 |
Nucleus | -2.73 | -13.65 | -10.30 | -10.96 | 4.06 | -3.29 | -5.44 | -8.33 | -5.71 | 1.37 | -5.24 | -19.70 | -15.99 | -12.35 | 6.65 |
Peroxisomes | -6.67 | 4.27 | -0.92 | 6.96 | -7.72 | -9.20 | 7.25 | 0.87 | 8.60 | -4.81 | -9.84 | 15.23 | 2.41 | 11.42 | -10.59 |
Vacuole | 13.14 | 13.41 | 0.79 | -26.08 | -27.02 | -12.35 | -10.17 | -21.97 | -16.76 | -5.18 | 1.63 | -2.20 | -25.96 | -30.75 | -26.53 |
Vacuole Periphery | -2.11 | 2.44 | -0.01 | 2.39 | -3.11 | -7.54 | 6.95 | 1.08 | 7.17 | -2.98 | -6.89 | 5.92 | -0.44 | 5.06 | -3.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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