Standard name | |
---|---|
Human Ortholog | |
Description | Guanine nucleotide exchange factor for the GTPase Gtr1p; subunit of the HOPS endocytic tethering complex; vacuole membrane protein; functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p; facilitates tethering and promotes membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; component of vacuole-mitochondrion contacts (vCLAMPs) important for lipid transfer between organelles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 |
Bud | 1 | 2 | 5 | 7 | 5 | 12 | 26 | 28 | 24 | 34 | 39 | 29 | 0 | 0 | 0 | 7 | 1 | 4 | 2 | 0 | 0 | 2 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 28 | 31 | 31 | 41 | 76 | 72 | 145 | 163 | 135 | 185 | 151 | 203 | 30 | 30 | 72 | 112 | 66 | 36 | 3 | 5 | 0 | 3 | 3 | 4 |
Endoplasmic Reticulum | 2 | 6 | 0 | 1 | 1 | 3 | 3 | 1 | 3 | 1 | 0 | 2 | 9 | 8 | 1 | 25 | 15 | 10 | 0 | 1 | 1 | 0 | 3 | 1 |
Endosome | 182 | 30 | 240 | 165 | 326 | 107 | 157 | 89 | 45 | 31 | 16 | 48 | 29 | 29 | 97 | 153 | 157 | 125 | 240 | 139 | 217 | 111 | 177 | 160 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 0 | 2 | 2 |
Mitochondria | 68 | 103 | 62 | 118 | 78 | 163 | 261 | 392 | 317 | 480 | 337 | 408 | 0 | 0 | 1 | 48 | 38 | 26 | 18 | 14 | 11 | 7 | 17 | 11 |
Nucleus | 12 | 15 | 2 | 1 | 3 | 1 | 4 | 10 | 2 | 5 | 0 | 2 | 22 | 22 | 13 | 14 | 12 | 7 | 5 | 6 | 1 | 4 | 3 | 2 |
Nuclear Periphery | 11 | 5 | 1 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 8 | 2 | 18 | 4 | 23 | 5 | 10 | 9 |
Nucleolus | 29 | 11 | 27 | 7 | 25 | 19 | 19 | 27 | 10 | 14 | 4 | 10 | 2 | 2 | 9 | 12 | 29 | 16 | 2 | 2 | 0 | 1 | 0 | 2 |
Peroxisomes | 0 | 0 | 15 | 6 | 19 | 5 | 27 | 18 | 11 | 16 | 2 | 12 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 33 | 4 | 61 | 33 | 62 | 32 | 53 | 65 | 39 | 39 | 26 | 43 | 16 | 16 | 41 | 61 | 63 | 23 | 4 | 6 | 10 | 5 | 5 | 3 |
Vac/Vac Membrane | 279 | 168 | 121 | 83 | 122 | 67 | 79 | 61 | 46 | 43 | 30 | 64 | 88 | 91 | 184 | 171 | 151 | 109 | 219 | 147 | 77 | 57 | 82 | 73 |
Unique Cell Count | 451 | 288 | 355 | 333 | 504 | 352 | 563 | 633 | 476 | 642 | 455 | 603 | 169 | 171 | 321 | 423 | 363 | 241 | 520 | 338 | 356 | 205 | 312 | 279 |
Labelled Cell Count | 646 | 375 | 565 | 462 | 721 | 481 | 775 | 856 | 636 | 850 | 606 | 823 | 200 | 202 | 419 | 606 | 544 | 359 | 520 | 338 | 356 | 205 | 312 | 279 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.0 | 5.4 | 5.1 | 5.2 | 4.4 | 4.1 | 4.0 | 3.9 | 3.4 | 3.6 | 3.7 | 6.1 | 6.1 | 6.4 | 4.9 | 5.6 | 6.9 | 6.1 | 6.6 | 6.9 |
Std Deviation (1e-4) | 0.6 | 0.8 | 1.6 | 1.6 | 1.9 | 1.5 | 1.4 | 1.1 | 1.3 | 1.1 | 0.9 | 0.9 | 0.7 | 0.7 | 0.8 | 1.1 | 1.3 | 1.7 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.05 | -0.3 | -0.39 | -0.44 | -0.46 | -0.65 | -0.59 | -0.54 | 0.18 | 0.18 | 0.25 | -0.12 | 0.06 | 0.36 | 0.18 | 0.29 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | 2.8 | 4.4 | 6.4 | 6.5 | 7.0 | 7.4 | 8.3 | 8.7 | 3.0 | 3.0 | 4.9 | 6.4 | 3.7 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 3.9 | 0 |
Endosome | -4.8 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -10.8 | -10.9 | -9.7 | -8.7 | -6.6 | -3.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.4 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.8 | -5.8 | -7.7 | -2.4 | -2.7 | -2.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 6.3 | 3.1 | 2.7 | 2.7 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.3 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.0 | -3.0 | -2.8 | -3.0 | 0.2 | -0.4 |
Peroxisomes | -1.8 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -4.3 | -4.0 | -3.2 |
SpindlePole | -2.8 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.3 | -2.4 | -1.6 | -1.1 | 0.1 | -2.6 |
Vacuole | -2.6 | -3.2 | -4.5 | -7.2 | -9.5 | -8.7 | -11.2 | -10.0 | -8.9 | 3.9 | 4.2 | 6.1 | 1.8 | 2.1 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8124 | 1.1826 | 1.0155 | 0.8493 | 1.3962 | 0.9665 | 2.0718 | 2.4266 | 2.5457 | 1.8021 | 2.4221 | 2.4091 | 2.9153 | 2.8546 | 2.096 | 1.0957 | 1.8063 | 2.2782 |
Actin | 0.0412 | 0.0075 | 0.0018 | 0.0008 | 0.0165 | 0.0006 | 0.0009 | 0 | 0.0207 | 0.0008 | 0.0039 | 0.002 | 0.0016 | 0 | 0.0002 | 0 | 0 | 0 |
Bud | 0.0013 | 0.0026 | 0.0026 | 0.0004 | 0.001 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0063 | 0.0049 | 0.003 | 0.0005 | 0.0007 | 0.0023 | 0.0008 | 0.0003 | 0.0008 | 0.0004 | 0.0007 | 0.0007 | 0.0017 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0005 |
Bud Periphery | 0.0027 | 0.0032 | 0.0045 | 0.0004 | 0.0022 | 0.001 | 0.0011 | 0.0004 | 0.0007 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0002 |
Bud Site | 0.0091 | 0.0069 | 0.0032 | 0.0007 | 0.0019 | 0.0011 | 0.0031 | 0.0007 | 0.0051 | 0.0007 | 0.0016 | 0.0003 | 0.0081 | 0.0011 | 0.0013 | 0.0002 | 0.0001 | 0.0004 |
Cell Periphery | 0.0009 | 0.0007 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0015 | 0.0018 | 0.0006 | 0 | 0 | 0.0003 |
Cytoplasm | 0.0113 | 0.0565 | 0.0275 | 0.0177 | 0.0082 | 0.0109 | 0.0037 | 0.0055 | 0.0087 | 0.0022 | 0.0105 | 0.0095 | 0.0397 | 0.0024 | 0.0436 | 0.0024 | 0.0015 | 0.0369 |
Cytoplasmic Foci | 0.0783 | 0.0832 | 0.0914 | 0.1214 | 0.0694 | 0.0749 | 0.0846 | 0.0811 | 0.0948 | 0.1136 | 0.0934 | 0.0885 | 0.0676 | 0.0456 | 0.0657 | 0.0684 | 0.1378 | 0.0614 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0007 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0076 | 0.0051 | 0.0067 | 0.0004 | 0.0029 | 0.0014 | 0.001 | 0.0015 | 0.0052 | 0.0003 | 0.0012 | 0.0011 | 0.0095 | 0.0006 | 0.0101 | 0.0004 | 0.0004 | 0.002 |
Endosome | 0.481 | 0.4693 | 0.5556 | 0.616 | 0.5616 | 0.5709 | 0.5752 | 0.6151 | 0.585 | 0.6442 | 0.5726 | 0.6399 | 0.1964 | 0.545 | 0.4848 | 0.6226 | 0.6808 | 0.5031 |
Golgi | 0.035 | 0.0156 | 0.0273 | 0.0236 | 0.0549 | 0.0281 | 0.0449 | 0.0237 | 0.0511 | 0.0452 | 0.0382 | 0.0375 | 0.0112 | 0.0119 | 0.0348 | 0.0266 | 0.0236 | 0.0277 |
Lipid Particles | 0.0551 | 0.0743 | 0.0433 | 0.0804 | 0.0955 | 0.0757 | 0.0579 | 0.0352 | 0.0708 | 0.0637 | 0.0528 | 0.0806 | 0.1987 | 0.1333 | 0.0376 | 0.0455 | 0.0893 | 0.1815 |
Mitochondria | 0.0259 | 0.005 | 0.0376 | 0.0791 | 0.0713 | 0.0051 | 0.0175 | 0.0155 | 0.0266 | 0.0071 | 0.0274 | 0.0065 | 0.003 | 0.0014 | 0.0096 | 0.014 | 0.0014 | 0.0017 |
None | 0.0496 | 0.036 | 0.0231 | 0.001 | 0.0015 | 0.0019 | 0.0018 | 0.0295 | 0.0017 | 0.0002 | 0.089 | 0.01 | 0.0861 | 0.0008 | 0.0544 | 0.0008 | 0.0002 | 0.0044 |
Nuclear Periphery | 0.0348 | 0.0138 | 0.0059 | 0.0004 | 0.0342 | 0.0084 | 0.0028 | 0.0016 | 0.0052 | 0.0005 | 0.0012 | 0.0017 | 0.0294 | 0.0006 | 0.0255 | 0.0008 | 0.0007 | 0.0512 |
Nucleolus | 0.0032 | 0.003 | 0.0033 | 0.0005 | 0.002 | 0.0035 | 0.0008 | 0.0005 | 0.0008 | 0.0012 | 0.0023 | 0.0009 | 0.0164 | 0.0072 | 0.0032 | 0.0004 | 0.0002 | 0.001 |
Nucleus | 0.0088 | 0.0048 | 0.0072 | 0.0004 | 0.0053 | 0.0116 | 0.0008 | 0.0007 | 0.0008 | 0.0007 | 0.0011 | 0.0007 | 0.0195 | 0.001 | 0.0044 | 0.0003 | 0.0001 | 0.0034 |
Peroxisomes | 0.0105 | 0.0074 | 0.0109 | 0.0072 | 0.0184 | 0.0161 | 0.0188 | 0.012 | 0.027 | 0.0225 | 0.0142 | 0.0144 | 0.0069 | 0.0076 | 0.0047 | 0.0059 | 0.0062 | 0.0114 |
Punctate Nuclear | 0.0054 | 0.0066 | 0.0014 | 0.0005 | 0.0056 | 0.0029 | 0.0008 | 0.0005 | 0.0008 | 0.0004 | 0.0041 | 0.0011 | 0.1635 | 0.0005 | 0.0017 | 0.0004 | 0.0004 | 0.0013 |
Vacuole | 0.1016 | 0.162 | 0.1165 | 0.0389 | 0.0331 | 0.147 | 0.1429 | 0.1343 | 0.0705 | 0.0837 | 0.0712 | 0.0797 | 0.1261 | 0.2164 | 0.1622 | 0.1688 | 0.046 | 0.0976 |
Vacuole Periphery | 0.0301 | 0.0316 | 0.0264 | 0.0096 | 0.0133 | 0.036 | 0.0394 | 0.0414 | 0.0222 | 0.0121 | 0.0123 | 0.0245 | 0.0122 | 0.0219 | 0.0545 | 0.0422 | 0.0111 | 0.0137 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.3843 | 10.9597 | 10.6886 | 17.8279 | 8.1749 | 11.203 | 9.5775 | 14.531 | 13.1623 | 15.1591 |
Translational Efficiency | 0.4618 | 0.4106 | 0.444 | 0.379 | 0.5234 | 0.4548 | 0.5532 | 0.2961 | 0.4128 | 0.3235 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1662 | 477 | 536 | 1356 | 1997 | 1185 | 2916 | 1739 | 3659 | 1662 | 3452 | 3095 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 807.75 | 811.85 | 998.84 | 934.90 | 681.17 | 804.17 | 893.00 | 870.70 | 738.67 | 806.37 | 909.43 | 898.83 |
Standard Deviation | 89.22 | 125.29 | 119.54 | 119.58 | 262.77 | 105.80 | 102.58 | 109.32 | 212.77 | 111.79 | 112.15 | 118.30 |
Intensity Change Log 2 | — | 0.007304 | 0.306345 | 0.210903 | — | 0.239486 | 0.390645 | 0.354161 | — | 0.118179 | 0.345524 | 0.278212 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000085 | 0.000197 | 0.000129 | 0.000184 | 0.000140 | 0.000164 | 0.000023 | 0.000095 | 0.000115 | 0.000174 | 0.000039 | 0.000134 |
Bud Neck | 0.015294 | 0.013118 | 0.007223 | 0.011403 | 0.029943 | 0.013395 | 0.022779 | 0.010321 | 0.023289 | 0.013315 | 0.020364 | 0.010795 |
Bud Site | 0.009764 | 0.016758 | 0.014340 | 0.026276 | 0.015515 | 0.005382 | 0.008025 | 0.016515 | 0.012903 | 0.008647 | 0.009005 | 0.020792 |
Cell Periphery | 0.000136 | 0.000081 | 0.000046 | 0.000064 | 0.000370 | 0.000151 | 0.000041 | 0.000175 | 0.000264 | 0.000131 | 0.000041 | 0.000126 |
Cytoplasm | 0.006964 | 0.036194 | 0.013092 | 0.019928 | 0.053723 | 0.010848 | 0.004196 | 0.011040 | 0.032484 | 0.018122 | 0.005577 | 0.014934 |
Cytoplasmic Foci | 0.018923 | 0.112968 | 0.112911 | 0.048774 | 0.134091 | 0.051875 | 0.014469 | 0.048377 | 0.081779 | 0.069409 | 0.029755 | 0.048551 |
Eisosomes | 0.000032 | 0.000031 | 0.000018 | 0.000013 | 0.000165 | 0.000037 | 0.000024 | 0.000016 | 0.000104 | 0.000036 | 0.000023 | 0.000015 |
Endoplasmic Reticulum | 0.001933 | 0.000780 | 0.000671 | 0.000780 | 0.005300 | 0.002788 | 0.001217 | 0.000611 | 0.003771 | 0.002212 | 0.001132 | 0.000685 |
Endosome | 0.455600* | 0.505361* | 0.424204* | 0.343116* | 0.399031* | 0.489498* | 0.394276* | 0.491690* | 0.424726* | 0.494051* | 0.398923* | 0.426596* |
Golgi | 0.006944 | 0.014835 | 0.036634 | 0.013468 | 0.042505 | 0.013964 | 0.006349 | 0.019666 | 0.026353 | 0.014214 | 0.011052 | 0.016950 |
Lipid Particles | 0.003383 | 0.006321 | 0.001979 | 0.000999 | 0.016264 | 0.013717 | 0.000765 | 0.001744 | 0.010413 | 0.011594 | 0.000954 | 0.001417 |
Mitochondria | 0.011990 | 0.009341 | 0.002145 | 0.005208 | 0.058105 | 0.021223 | 0.006975 | 0.007821 | 0.037159 | 0.017813 | 0.006225 | 0.006676 |
Mitotic Spindle | 0.008012 | 0.020308 | 0.052025 | 0.019791 | 0.000515 | 0.005840 | 0.008905 | 0.014295 | 0.003920 | 0.009993 | 0.015600 | 0.016703 |
None | 0.000251 | 0.000538 | 0.000095 | 0.000193 | 0.002417 | 0.000508 | 0.000231 | 0.000136 | 0.001433 | 0.000517 | 0.000210 | 0.000161 |
Nuclear Periphery | 0.003260 | 0.000596 | 0.001760 | 0.000924 | 0.000274 | 0.001677 | 0.001998 | 0.000739 | 0.001631 | 0.001367 | 0.001961 | 0.000820 |
Nuclear Periphery Foci | 0.002988 | 0.004755 | 0.014641 | 0.001855 | 0.000310 | 0.005713 | 0.002876 | 0.001053 | 0.001526 | 0.005438 | 0.004702 | 0.001405 |
Nucleolus | 0.013925 | 0.002907 | 0.004252 | 0.002363 | 0.001114 | 0.005092 | 0.020958 | 0.003087 | 0.006933 | 0.004465 | 0.018364 | 0.002770 |
Nucleus | 0.019819 | 0.004471 | 0.003983 | 0.004940 | 0.006119 | 0.009579 | 0.021212 | 0.003137 | 0.012342 | 0.008113 | 0.018537 | 0.003927 |
Peroxisomes | 0.006634 | 0.011264 | 0.017108 | 0.006646 | 0.004544 | 0.009271 | 0.002392 | 0.006280 | 0.005493 | 0.009843 | 0.004677 | 0.006440 |
Vacuole | 0.364036* | 0.196088 | 0.240234* | 0.442230* | 0.161563 | 0.267330* | 0.404984* | 0.313115* | 0.253531* | 0.246884* | 0.379402* | 0.369684* |
Vacuole Periphery | 0.050028 | 0.043089 | 0.052509 | 0.050845 | 0.067990 | 0.071946 | 0.077303 | 0.050088 | 0.059831 | 0.063664 | 0.073453 | 0.050420 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.26 | -2.26 | -2.62 | 0.13 | -0.82 | -0.19 | 3.09 | 0.92 | 0.56 | -2.20 | -0.65 | 3.21 | -0.65 | 0.42 | -3.74 |
Bud Neck | 0.91 | 6.25 | 2.34 | 0.29 | -4.58 | 6.81 | 2.74 | 9.04 | 1.72 | 10.61 | 5.95 | 1.42 | 9.09 | 1.43 | 9.74 |
Bud Site | -2.62 | -2.74 | -6.62 | -2.93 | -4.36 | 6.78 | 5.19 | -0.68 | -6.88 | -5.43 | 3.30 | 3.47 | -5.38 | -7.79 | -8.34 |
Cell Periphery | 1.23 | 2.07 | 1.61 | 1.03 | -2.08 | 4.53 | 8.60 | 1.62 | -0.17 | -1.12 | 3.73 | 7.80 | 1.93 | 0.08 | -1.27 |
Cytoplasm | -5.53 | -3.01 | -7.93 | 1.99 | -3.40 | 14.49 | 18.73 | 14.41 | -0.87 | -5.99 | 5.92 | 16.36 | 7.47 | 0.05 | -9.43 |
Cytoplasmic Foci | -15.09 | -17.45 | -12.92 | 10.11 | 11.31 | 19.70 | 36.57 | 23.57 | 1.37 | -19.15 | 3.39 | 22.50 | 13.84 | 7.46 | -11.02 |
Eisosomes | -0.15 | 5.42 | 10.43 | 3.23 | 3.29 | 11.45 | 13.25 | 14.05 | 6.32 | 7.01 | 10.29 | 13.55 | 15.08 | 7.21 | 9.43 |
Endoplasmic Reticulum | 5.33 | 6.26 | 5.73 | -0.16 | -0.71 | 3.17 | 7.11 | 8.19 | 3.77 | 6.84 | 3.06 | 8.02 | 9.44 | 3.66 | 5.99 |
Endosome | -3.09 | 2.83 | 12.09 | 11.60 | 6.96 | -7.83 | 1.19 | -9.09 | 0.33 | -10.76 | -7.39 | 4.38 | 1.31 | 8.27 | -2.94 |
Golgi | -4.73 | -10.60 | -4.23 | 1.07 | 7.90 | 12.16 | 18.76 | 10.62 | -3.13 | -12.19 | 7.90 | 12.73 | 7.28 | -1.66 | -5.92 |
Lipid Particles | -1.77 | 2.58 | 5.03 | 3.21 | 3.80 | 1.11 | 14.21 | 12.36 | 7.50 | -2.52 | -1.09 | 14.76 | 13.09 | 8.32 | -2.19 |
Mitochondria | 1.20 | 7.18 | 4.37 | 2.06 | -3.22 | 12.76 | 21.04 | 20.04 | 7.34 | -0.82 | 9.85 | 20.09 | 19.16 | 7.88 | -0.42 |
Mitotic Spindle | -2.59 | -7.31 | -5.02 | -0.50 | 4.64 | -4.21 | -9.32 | -8.66 | -4.23 | -2.84 | -3.59 | -8.77 | -9.06 | -3.85 | -1.11 |
None | -1.02 | 3.51 | 0.94 | 1.21 | -1.93 | 2.33 | 2.71 | 2.84 | 3.52 | 4.09 | 1.99 | 2.77 | 2.89 | 3.23 | 2.06 |
Nuclear Periphery | 3.93 | 1.77 | 3.28 | -2.51 | 1.37 | -4.54 | -7.73 | -3.60 | 2.35 | 4.57 | 0.73 | -1.25 | 2.10 | 1.67 | 4.84 |
Nuclear Periphery Foci | -1.12 | -3.62 | 0.79 | 1.44 | 3.82 | -4.19 | -5.12 | -4.52 | 3.55 | 3.19 | -3.73 | -4.48 | -0.51 | 3.61 | 4.32 |
Nucleolus | 7.09 | 5.74 | 8.06 | 0.64 | 1.94 | -4.71 | -16.84 | -4.39 | 2.40 | 14.70 | 1.99 | -10.00 | 5.57 | 2.69 | 15.30 |
Nucleus | 6.83 | 7.16 | 6.26 | -1.17 | -1.37 | -2.05 | -10.04 | 2.41 | 4.40 | 14.27 | 2.98 | -4.74 | 6.64 | 3.12 | 12.66 |
Peroxisomes | -3.03 | -5.41 | 0.33 | 3.13 | 5.45 | -4.55 | 5.85 | -3.04 | 2.85 | -7.86 | -4.99 | 2.00 | -1.54 | 3.98 | -3.31 |
Vacuole | 13.80 | 10.22 | -8.51 | -20.49 | -17.26 | -11.66 | -38.92 | -21.12 | -5.19 | 11.45 | 1.02 | -20.92 | -18.93 | -16.28 | 0.57 |
Vacuole Periphery | 1.71 | -0.37 | 0.63 | -1.28 | 0.77 | -0.91 | -2.93 | 6.24 | 6.01 | 10.32 | -1.19 | -5.73 | 5.28 | 5.02 | 11.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|