Standard name
Human Ortholog
Description Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0 – 0.05 0.08 0.12 0.23 – 0.12 0.14 0.14 0.15 0.05 0.16 0.24 0.14 0.13 0.18 0 0 0 0.09 0.06 0.06
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 – 0.05 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 – 0.05 0 0.07 0.12 – 0.24 0.23 0.48 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.79 0.88 – 0.84 0.84 0.72 0.61 – 0.6 0.6 0.51 0.55 0.85 0.79 0.71 0.67 0.71 0.57 0.75 0.86 0.59 0.65 0.59 0.6
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0.16 0 0 0 0.05 0.05 0 0 0 0 0.05 0.05
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07
Vac/Vac Membrane 0.06 0.12 – 0 0.07 0.12 0.12 – 0.1 0.08 0.12 0.12 0 0.05 0.08 0.16 0.11 0.14 0.08 0.06 0.19 0.09 0.12 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 1 1 2 2 0 0 0 0 1 0 2 4 2 6 10 9
Bud Neck 0 0 – 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 – 0 0 0 3 – 3 2 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 10 – 1 9 16 34 – 15 18 19 22 5 35 42 29 25 30 1 4 2 11 12 15
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 2 0 0 0 0 0 10 4 5 0 0 0 0 1 0
Endosome 0 3 – 1 2 4 7 – 4 1 0 2 0 2 3 4 2 7 2 0 0 0 4 3
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 5 3 5 0 1 1 1 0 0
Mitochondria 10 1 – 1 2 9 18 – 29 30 64 51 0 0 0 2 1 2 0 0 0 0 3 0
Nucleus 42 200 – 16 99 98 92 – 72 78 68 81 87 172 123 135 137 96 54 209 30 85 121 139
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 6 – 0 2 2 4 – 4 1 2 1 16 4 1 3 9 8 3 5 1 3 9 12
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 4 2 1 0 0 0 0 0 0 1 1 2 1 7 12 15
Vac/Vac Membrane 3 27 – 0 8 17 18 – 12 11 16 17 2 10 13 33 22 24 6 13 9 11 23 29
Unique Cell Count 53 227 19 118 137 150 121 131 133 147 102 218 173 203 194 167 73 245 52 131 205 232
Labelled Cell Count 62 247 19 122 146 176 144 147 174 176 110 223 182 221 204 179 73 245 52 131 205 232


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 8.2 – 8.6 8.7 6.8 6.6 – 6.0 5.8 5.2 5.3 8.2 8.9 7.7 14.0 15.2 13.8 6.3 6.5 7.1
Std Deviation (1e-4) 0.9 1.4 – 4.4 2.3 1.9 1.6 – 2.1 2.0 1.4 1.6 1.8 2.4 1.7 4.7 4.3 3.9 0.8 1.2 1.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.838 4.9451 4.6461 4.7246 3.3285 4.9212 4.7789 6.0958 5.2145 4.9003 3.612 5.4086 4.7578 6.3582 5.6687 4.4324 4.8895 5.6805
Actin 0.0186 0.0002 0.002 0.0001 0.0522 0.0011 0.0085 0.0067 0 0.003 0.0098 0.0004 0.0425 0.0005 0.0042 0.0126 0.0462 0.003
Bud 0.0005 0 0.0003 0 0.0013 0.0001 0.0009 0.0001 0.0001 0.0001 0.0012 0.0001 0.0009 0.0007 0.0002 0.0004 0.0039 0.0002
Bud Neck 0.0047 0.0001 0.0004 0.0001 0.0027 0.0018 0.0013 0.0006 0.0001 0.0001 0.0022 0.0012 0.0015 0.0004 0.0004 0.0012 0.0051 0.0013
Bud Periphery 0.0009 0 0.0007 0 0.0015 0.0001 0.0024 0.0001 0.0001 0.0001 0.006 0.0001 0.0027 0.0008 0.0003 0.0005 0.0068 0.0003
Bud Site 0.0042 0.0001 0.0016 0 0.0133 0.0001 0.0027 0.0101 0.0002 0.0001 0.0027 0.0001 0.0019 0.0026 0.0014 0.0011 0.0088 0.0002
Cell Periphery 0.0006 0 0.0001 0 0.0004 0 0.0005 0.0005 0 0 0.0027 0 0.001 0.0002 0.0001 0.0001 0.0017 0.0001
Cytoplasm 0.0084 0.0001 0.0015 0.0005 0.0015 0.0001 0.0099 0.0008 0.0003 0.0015 0.0017 0.0003 0.01 0.0021 0.002 0.0027 0.0076 0.0005
Cytoplasmic Foci 0.0279 0.001 0.0042 0 0.0187 0.0007 0.0085 0.0002 0.0029 0.011 0.0412 0.0003 0.0098 0.0005 0.0051 0.0313 0.0149 0.0035
Eisosomes 0.0002 0 0.0001 0 0.0003 0 0.0001 0.0003 0 0 0.0004 0 0.0002 0 0.0001 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.002 0 0.0013 0 0.0005 0.0002 0.0018 0.0003 0.0001 0.0033 0.0039 0.0001 0.0034 0.0003 0.0059 0.0005 0.0054 0.0007
Endosome 0.017 0.0019 0.0086 0 0.0172 0.0046 0.0059 0.0002 0.0058 0.0159 0.0172 0.0033 0.0201 0.0012 0.0107 0.0253 0.0317 0.0106
Golgi 0.0105 0.0033 0.0039 0 0.0208 0.002 0.0023 0.0003 0.0012 0.003 0.0109 0.0062 0.0141 0.0004 0.0025 0.0532 0.0328 0.0086
Lipid Particles 0.0264 0.0006 0.0061 0 0.0292 0.003 0.0034 0.0001 0.0005 0.0166 0.0485 0.0001 0.013 0.0001 0.0065 0.0504 0.0068 0.0039
Mitochondria 0.0084 0.007 0.0042 0 0.0095 0.0008 0.0065 0.0004 0.0002 0.0009 0.0857 0.0014 0.0281 0.0025 0.0008 0.0245 0.025 0.0134
None 0.0045 0 0.0005 0 0.0006 0.0001 0.002 0.0001 0.0001 0.0007 0.0048 0.0002 0.0029 0.0003 0.0012 0.0003 0.0011 0.0014
Nuclear Periphery 0.0077 0.0007 0.0026 0.0054 0.0028 0.0018 0.0105 0.0007 0.0006 0.0511 0.0047 0.0007 0.0116 0.0021 0.0019 0.0042 0.0131 0.0015
Nucleolus 0.0156 0.0068 0.0092 0.0032 0.0486 0.0247 0.0154 0.0069 0.0097 0.0024 0.0387 0.0195 0.0306 0.0132 0.004 0.0036 0.0356 0.0205
Nucleus 0.8015 0.9767 0.9484 0.9728 0.7474 0.9573 0.8901 0.971 0.9753 0.8696 0.6772 0.9604 0.7778 0.9683 0.9456 0.7471 0.7279 0.9236
Peroxisomes 0.0155 0.0009 0.002 0 0.0231 0.0004 0.0046 0.0001 0.0003 0.0028 0.0229 0 0.0125 0.0001 0.0044 0.0264 0.0053 0.0014
Punctate Nuclear 0.012 0.0002 0.0014 0.0177 0.0042 0.0008 0.0129 0.0003 0.0019 0.0136 0.0087 0.0055 0.005 0.0008 0.0019 0.0129 0.0028 0.0039
Vacuole 0.0098 0.0001 0.0007 0 0.0032 0.0003 0.0071 0.0001 0.0006 0.0016 0.0059 0.0002 0.0072 0.0022 0.0006 0.0009 0.0135 0.0005
Vacuole Periphery 0.0034 0.0001 0.0004 0 0.0012 0.0002 0.0028 0 0.0001 0.0025 0.0029 0.0001 0.0032 0.0006 0.0003 0.0007 0.0035 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.1983 60.2014 53.0719 46.7214 53.3588 54.6507 65.1616 65.4867 55.5368 63.3445
Translational Efficiency 1.1466 0.8629 0.7494 0.7356 0.8259 1.1391 0.8176 0.7067 0.7662 0.6969

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2116 1860 368 1319 1600 2136 245 12 3716 3996 613 1331

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.47 853.85 1428.91 1093.84 817.21 855.51 1320.96 338.02 722.83 854.74 1385.77 1087.03
Standard Deviation 81.65 130.33 205.14 186.57 95.93 127.17 188.01 4.34 120.39 128.65 205.40 198.99
Intensity Change Log 2 — 0.390284 1.133144 0.747631 — 0.066078 0.692808 -1.273598 — 0.218936 0.904843 -0.036630

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000124 0.000484 0.001755 0.001013 0.000281 0.000426 0.001531 0.000076 0.000191 0.000453 0.001665 0.001005
Bud Neck 0.005965 0.010582 0.026348 0.030416 0.003838 0.013325 0.012866 0.000004 0.005049 0.012048 0.020960 0.030141
Bud Site 0.003862 0.001676 0.002585 0.008973 0.000788 0.002362 0.001693 0.000018 0.002538 0.002043 0.002229 0.008892
Cell Periphery 0.000363 0.000139 0.000099 0.000266 0.000140 0.000112 0.000088 0.000223 0.000267 0.000124 0.000095 0.000266
Cytoplasm 0.045529 0.031935 0.000840 0.026515 0.016358 0.009187 0.000846 0.000027 0.032969 0.019775 0.000842 0.026276
Cytoplasmic Foci 0.006351 0.002048 0.000105 0.002380 0.002675 0.001251 0.000089 0.000007 0.004768 0.001622 0.000099 0.002359
Eisosomes 0.000030 0.000021 0.000064 0.000029 0.000026 0.000022 0.000067 0.000402 0.000028 0.000021 0.000065 0.000033
Endoplasmic Reticulum 0.001764 0.004492 0.003614 0.003051 0.002877 0.003805 0.004992 0.000001 0.002243 0.004125 0.004165 0.003023
Endosome 0.000789 0.000848 0.000183 0.003284 0.000749 0.000763 0.000149 0.000000 0.000772 0.000803 0.000169 0.003254
Golgi 0.000175 0.000229 0.000153 0.004345 0.000259 0.000429 0.000117 0.000003 0.000211 0.000336 0.000139 0.004306
Lipid Particles 0.001203 0.000672 0.000157 0.000178 0.001348 0.000624 0.000128 0.000011 0.001265 0.000647 0.000145 0.000176
Mitochondria 0.001532 0.001840 0.003671 0.007104 0.001583 0.004164 0.001889 0.000010 0.001554 0.003082 0.002959 0.007040
Mitotic Spindle 0.000515 0.003399 0.000357 0.047423 0.001916 0.005064 0.001459 0.000124 0.001119 0.004289 0.000797 0.046997
None 0.027005 0.007344 0.001116 0.014160 0.006415 0.006646 0.002414 0.998933* 0.018140 0.006971 0.001635 0.023039
Nuclear Periphery 0.001198 0.002011 0.001018 0.001989 0.000821 0.001783 0.000345 0.000000 0.001036 0.001889 0.000749 0.001971
Nuclear Periphery Foci 0.000530 0.000727 0.000284 0.001402 0.000600 0.001014 0.000075 0.000001 0.000560 0.000881 0.000200 0.001389
Nucleolus 0.025976 0.012444 0.009556 0.007999 0.016521 0.025641 0.013435 0.000001 0.021905 0.019498 0.011106 0.007927
Nucleus 0.873937* 0.915199* 0.943760* 0.826786* 0.940672* 0.919160* 0.954324* 0.000005 0.902671* 0.917316* 0.947982* 0.819332*
Peroxisomes 0.001066 0.000553 0.000867 0.000386 0.000572 0.000859 0.000846 0.000153 0.000854 0.000717 0.000859 0.000384
Vacuole 0.001759 0.002601 0.003002 0.009206 0.001328 0.002554 0.002510 0.000000 0.001573 0.002576 0.002805 0.009123
Vacuole Periphery 0.000327 0.000755 0.000466 0.003095 0.000232 0.000810 0.000138 0.000000 0.000286 0.000784 0.000335 0.003067

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.32 -15.14 -18.41 -11.04 4.01 NaN NaN NaN NaN NaN -10.67 -20.50 -17.10 -12.65 5.03
Bud Neck -5.43 -8.53 -14.73 -11.41 -0.62 NaN NaN NaN NaN NaN -11.35 -10.49 -15.85 -10.91 -2.94
Bud Site 2.35 0.22 -3.13 -6.57 -4.12 NaN NaN NaN NaN NaN 0.61 -1.11 -5.16 -6.03 -4.97
Cell Periphery 3.19 3.59 2.48 -2.17 -3.79 NaN NaN NaN NaN NaN 3.32 3.99 1.84 -2.70 -4.49
Cytoplasm 4.11 18.29 7.98 3.97 -9.98 NaN NaN NaN NaN NaN 6.60 20.59 5.13 -0.04 -9.97
Cytoplasmic Foci 4.90 7.92 6.69 3.14 -2.18 NaN NaN NaN NaN NaN 6.02 9.69 7.24 2.66 -2.25
Eisosomes 3.13 -9.43 -4.52 -11.30 7.39 NaN NaN NaN NaN NaN 4.22 -13.68 -6.76 -11.16 7.93
Endoplasmic Reticulum -9.87 -6.37 -11.08 0.59 -1.58 NaN NaN NaN NaN NaN -10.03 -8.35 -9.36 -0.74 0.36
Endosome -0.66 4.13 -1.73 -1.37 -4.29 NaN NaN NaN NaN NaN -0.28 5.95 -1.73 -1.64 -4.33
Golgi -0.57 -0.40 -1.81 -1.72 -1.79 NaN NaN NaN NaN NaN -1.24 0.83 -1.74 -1.55 -1.81
Lipid Particles 2.67 6.15 5.68 5.17 -2.62 NaN NaN NaN NaN NaN 3.57 7.86 7.24 4.02 -3.36
Mitochondria -0.94 -4.42 -3.15 -2.76 -0.44 NaN NaN NaN NaN NaN -2.96 -4.46 -3.10 -1.08 -1.13
Mitotic Spindle -3.44 0.18 -8.88 -7.72 -8.90 NaN NaN NaN NaN NaN -4.35 -0.11 -8.65 -7.48 -8.50
None 10.37 14.42 8.23 -1.86 -7.66 NaN NaN NaN NaN NaN 9.16 14.98 -0.10 -4.11 -6.12
Nuclear Periphery -2.50 1.00 -2.23 0.69 -3.75 NaN NaN NaN NaN NaN -3.81 1.56 -3.77 0.46 -5.67
Nuclear Periphery Foci -1.56 1.89 -0.46 1.10 -2.51 NaN NaN NaN NaN NaN -1.49 3.93 -0.09 1.31 -3.10
Nucleolus 8.16 8.32 12.00 4.32 2.14 NaN NaN NaN NaN NaN 2.15 6.62 12.30 10.68 3.34
Nucleus -6.63 -9.95 1.97 7.56 10.41 NaN NaN NaN NaN NaN -3.33 -9.01 7.20 9.29 12.64
Peroxisomes 1.66 0.37 3.14 2.38 6.08 NaN NaN NaN NaN NaN 0.72 -0.02 3.98 2.71 7.78
Vacuole -2.52 -3.24 -7.89 -7.04 -6.01 NaN NaN NaN NaN NaN -3.28 -4.65 -8.10 -7.19 -6.47
Vacuole Periphery -1.19 -0.14 -2.05 -0.58 -1.89 NaN NaN NaN NaN NaN -2.46 0.18 -2.19 -0.61 -2.20
Standard name
Human Ortholog
Description Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Bdf2

Bdf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bdf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available